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Affiliation |
Faculty of Agriculture Region of Marine Life Science |
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Title |
Associate Professor |
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External Link |
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Related SDGs |
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KONO Tomoya
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Research Areas 【 display / non-display 】
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Life Science / Aquatic bioproduction science
Papers 【 display / non-display 】
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Functional divergence of IL-17A/F and differential regulation by IL-17RC isoforms in Japanese medaka, Oryzias latipes Reviewed
Mizanur Rahman Washim, Chikara Irishika, Kosei Kawaguchi, Nanaki Harada, Yo Okamura, Tomoya Kono, Jun-ichi Hikima
Comparative Immunology Reports 11 200322 2026.12
Language：English Publishing type：Research paper (scientific journal)
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Expression dynamics of immune-related genes in circadian regulator Bmal1-deficient medaka (Oryzias latipes) Reviewed
Takahiko Hata, Kazutada Tsuda, Miyu Hirayoshi, Hiroshi Miyanishi, Jun-ichi Hikima, Tomoya Kono
Fish and Shellfish Immunology 176 111416 2026.9
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)
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Time-of-day-dependent host defense against Edwardsiella infection in medaka Reviewed
Takahiko Hata, Miyu Hirayoshi, Hiyori Iwai, Yuiko Kuroyanagi, Minato Funakura, Jun-ichi Hikima, Tomoya Kono
Journal of Fish Diseases 0:e70226 2026.7
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)
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Edwardsiella virulence protein P modulates immune response and cell death in Japanese medaka, Oryzias latipes Reviewed International coauthorship
Takechiyo Sumiyoshi, Aki Nishihara, Chikara Irishika, Shinya Yasumoto, Masakazu Kondo, Chan-Il Park, Tomoya Kono and Jun-ichi Hikima
Comparative Immunology Reports 10 200289 2026.6
Language：English Publishing type：Research paper (scientific journal)
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Washim M.R., Morimoto N., Sumiyoshi T., Nishihara A., Nallaperumal S., Kono T., Hikima J.i.
Fish and Shellfish Immunology 171 2026.4
Language：English Publishing type：Research paper (scientific journal) Publisher：Fish and Shellfish Immunology
Interleukin-1β is a pro-inflammatory cytokine with pluripotent roles in mediating immune response. In fish, IL-1β plays a major role in inflammatory responses to pathogenic infections, though its immunological functions through direct stimulation have not been studied in detail. In this study, two IL-1β genes (il1b1 and il1b2) from the Japanese medaka (Oryzias latipes) were identified and characterized. The broad immunostimulatory effects of recombinant IL-1β (OlrIL-1β) from Japanese medaka were explored using embryonic fibroblast-like cell line (OLHdrR-e3) and primary cultured kidney cells. Recombinant proteins were produced via prokaryotic expression based on predicted mature isoforms IL-1β1_Asp<sup>95</sup> (type II) and IL-1β2_Asp<sup>121</sup> (type I) to investigate their distinct functional characteristics. Then, their immune-related gene expression, phagocytosis, and reactive oxygen species (ROS) production ability were evaluated in vitro and ex vivo. rIL-1β triggers the expression of not only pro-inflammatory cytokines such as il1b, tnfa, il6, il8, mcp1b, and csf1b, but also the antimicrobial peptide, def1b. Additionally, they upregulate the expression of cell specific markers, including macrophage- and granulocyte-specific genes mpeg1.1, mpx2, and epx, and nitric oxide synthase (NO) gene nos1. Moreover, medaka OlrIL-1β proteins exhibited robust phagocytosis and superoxide anion production ability than those in unstimulated control. In all evaluations, the OlrIL-1β proteins corresponding to the predicted cleavage sites exhibited strong immune activation, with type I OlIL-1β2_Asp<sup>121</sup> demonstrating more potent response than type II. To our knowledge, this is the study to reveal the immunomodulatory functions of Japanese medaka IL-1β, highlighting its potential in immunotherapy for disease prevention and control in aquaculture.
Books 【 display / non-display 】
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月刊「養殖」サイトカインを用いた魚類免疫賦活剤の効果判定
酒井正博・高山博章・河野智哉（ Role： Joint author）
緑書房 2012.2
Responsible for pages：54-57 Language：Japanese
MISC 【 display / non-display 】
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Nishihara Aki, Sumiyoshi Takechiyo, Arakawa Koushiro, Morimoto Natsuki, Yasumoto Shinya, Kondo Masakazu, Kono Tomoya, Sakai Masahiro, Hikima Jun-ichi
Fish Pathology 58 ( 4 ) 175 - 179 2023
Language：English Publishing type：Rapid communication, short report, research note, etc. (scientific journal) Publisher：The Japanese Society of Fish Pathology
<i>Edwardsiella</i> bacteria infect Japanese medaka <i>Oryzias latipes</i>; however, the difference in susceptibility between <i>E. piscicida</i> and <i>E. anguillarum</i> is unknown. In this study, we performed challenge tests with medaka using four <i>Edwardsiella</i> strains, which were difficult to be identified by specific primers. The four strains were reclassified as three <i>E. piscicida</i> strains and one <i>E. anguillarum</i> strain using phylogenetic analysis. The immersion challenge demonstrated that medaka were more susceptible to <i>E. piscicida</i> than to <i>E. anguillarum</i>. Increases in the bacterial copy number in the infected medaka kidneys were correlated with the worsening of the external symptoms. Medaka is considered a valuable model organism for understanding the etiology of edwardsiellosis.
DOI： 10.3147/JSFP.58.175
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サイトカイン遺伝子を用いた魚類免疫賦活剤の効果判定
酒井正博、高山博章、河野智哉
養殖 2012.2
Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media) Publisher：緑書房
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Genomics of fish IL-17 ligand and receptors: A review.
T. Kono, H. Korenaga and M. Sakai
Fish and Shellfish Immunology ( 31 ) 635 - 643 2010.12
Language：English Publishing type：Article, review, commentary, editorial, etc. (scientific journal) Publisher：Elsevier
Presentations 【 display / non-display 】
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メダカ・ガスダーミンEの構造的特徴およびパイロトーシスの誘導
三柴時哉、岡本俊暁、河野智哉、引間順一
令和8年度日本魚病学会秋季大会 2026.9.18
Event date： 2026.9.17 - 2026.9.18
Language：Japanese Presentation type：Oral presentation (general)
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病原微生物および付着物に対する銅合金製生簀の有用性に関する研究
田林藍、荒川孝志郎、澤田梨久、中島智優、室谷冬香、宮村和良、野田誠、西山雅人、福田穣、阿川泰夫、和田葉子、河野智哉、引間順一
令和8年度日本水産学会秋季大会 2026.9.16
Event date： 2026.9.14 - 2026.9.17
Language：Japanese Presentation type：Oral presentation (general)
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銅合金属製生簀網の防汚・抗菌性能に関する調査
荒川孝志朗、澤田梨久、中島智優、室谷冬香、宮村和良、西山雅人、福田穣、阿川泰夫、白樫正、河野智哉、引間順一
令和8年度日本魚病学会春季大会
Event date： 2026.3.7 - 2026.3.8
Presentation type：Poster presentation
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Circadian regulation of Fish Immunology 魚類における免疫リズムの制御
羽田崇彦、平吉美優、引間順一、河野智哉
第32回日本時間生物学会学術大会
Event date： 2025.10.4 - 2025.10.5
Presentation type：Poster presentation
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Edwardsiella piscicida由来TIR結合因子TcpLの機能解明
入鹿誓良、住吉竹千代、近藤昌和、安本信哉、河野智哉、引間順一
令和7年度日本魚病学会秋季大会
Event date： 2025.9.11 - 2025.9.12
Presentation type：Oral presentation (general)
Awards 【 display / non-display 】
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農学特別賞 (研究部門)
2025.2 宮崎大学農学部
羽田崇彦、河野智哉
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2022年 宮崎大学学生農学特別賞表
2023.2 宮崎大学農学部 Diel rhythm of the inflammatory cytokine il1b in the Japanese medaka (Oryzias latipes) regulated by core components of the circadian clock
Tomoya Takeuchi, Takahiko Hata, Hiroshi Miyanishi, Takumi Yuasa, Suzuka Setoguchi, Ayaka Takeda, Natsuki Morimoto, Jun-ichi Hikima, Masahiro Sakai and Tomoya Kono
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日本水産学会 水産学進歩賞
2021.3 日本水産学会 魚類サイトカインによる自然免疫応答の概日リズム制御に関する研究
河野智哉
Award type：Award from Japanese society, conference, symposium, etc. Country：Japan
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宮崎大学農学部論文表彰
2018.1 宮崎大学農学部 Identification and immunoregulatory function of neuromedin U (Nmu) in the Japanese pufferfish Takifugu rubripes
河野智哉
Country：Japan
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北里大学同窓会研究奨励賞
2012.9 北里大学 魚類生理活性ペプチドのクロストークによる免疫制御機構の解析
河野智哉
Grant-in-Aid for Scientific Research 【 display / non-display 】
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βバレル型膜タンパク質輸送装置が周辺から受ける影響の解析
Grant number：25KF0261 2025.11 - 2028.03
独立行政法人日本学術振興会 科学研究費基金 特別研究員奨励費
Authorship：Principal investigator
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魚類と病原体が織りなす感染の概日リズムの究明 ～感染症の防除を目指して～
Grant number：24K01837 2024.04 - 2028.03
独立行政法人日本学術振興会 科学研究費基金 基盤研究(B)
Authorship：Principal investigator
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生物時計が調節する魚類の免疫リズムの解明 ～感染症の防除を目指して～
Grant number：20H03064 2020.04 - 2024.03
独立行政法人日本学術振興会 科学研究費補助金 基盤研究(B)
Authorship：Principal investigator
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フグはなぜ疾病に強いのか？フグの耐病性のメカニズムを探る
Grant number：17H01486 2017.04 - 2021.03
科学研究費補助金 基盤研究(A)
Authorship：Coinvestigator(s)
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魚類の免疫応答における概日リズムの解明～免疫増強剤の効果向上を目指して～
Grant number：17K07915 2017.04 - 2020.03
科学研究費補助金 基盤研究(C)
Authorship：Principal investigator
Available Technology 【 display / non-display 】
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魚類の免疫リズムの解明
魚病の防除を目的とした時間治療法の確立Home Page： 免疫生物学研究室
Related fields where technical consultation is available：時間免疫学に基づく：
・水産用ワクチンの開発～評価
・魚類免疫賦活剤の評価
・魚類の健康診断Message：・共同研究の希望テーマ：魚類の免疫力向上に関する研究