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Affiliation |
Faculty of Agriculture Region of Marine Life Science |
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Associate Professor |
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Related SDGs |
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KONO Tomoya
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Research Areas 【 display / non-display 】
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Life Science / Aquatic bioproduction science
Papers 【 display / non-display 】
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Yamamoto J., Deguchi H., Sumiyoshi T., Nakagami K., Saito A., Miyanishi H., Kondo M., Kono T., Sakai M., Kinoshita M., Hikima J.I.
Marine Biotechnology 26 ( 4 ) 658 - 671 2024.8
Publishing type：Research paper (scientific journal) Publisher：Marine Biotechnology
Intracellular bacteria such as those belonging to the genus Edwardsiella can survive and proliferate within macrophages. However, the detailed mechanisms underlying the host macrophage immune response and pathogen evasion strategies remain unknown. To advance the field of host macrophage research, we successfully established transgenic (Tg) Japanese medaka Oryzias latipes that possesses fluorescently visualized macrophages. As a macrophage marker, the macrophage-expressed gene 1.1 (mpeg1.1) was selected because of its predominant expression across various tissues in medaka. To validate the macrophage characteristics of the fluorescently labeled cells, May-Grünwald Giemsa staining and peroxidase staining were conducted. The labeled cells exhibited morphological features consistent with those of monocyte/macrophage-like cells and tested negative for peroxidase activity. Through co-localization studies, the fluorescently labeled cells co-localized with E. piscicida in the intestines and kidneys of infected medaka larvae, confirming the ingestion of bacteria through phagocytosis. In addition, the labeled cells expressed macrophage markers but lacked a neutrophil marker. These results suggested that the fluorescently labeled cells of Tg[mpeg1.1:mCherry/mAG] medaka were monocytes/macrophages, which will be useful for future studies aimed at understanding the mechanisms of macrophage-mediated bacterial infections.
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Accumulation and Phagocytosis of Fluorescently Visualized Macrophages Against Edwardsiella piscicida Infection in Established mpeg1.1‑Transgenic Japanese Medaka Oryzias latipes Reviewed
Juna Yamamoto, Hana Deguchi, Takechiyo Sumiyoshi, Kentaro Nakagami, Akatsuki Saito, Hiroshi Miyanishi, Masakazu Kondo, Tomoya Kono, Masahiro Sakai1, Masato Kinoshita, Jun‑ichi Hikima
Marine Biotechnology 26 658 - 671 2024.6
Language：English Publishing type：Research paper (scientific journal)
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Comprehensive analysis of diel rhythmic expression of the medaka tlr gene family Reviewed
Takahiko Hata, Suzuka Setoguchi, Natuki Morimoto, Hidetoshi Shimawaki, Jun-ichi Hikima, Masahiro Sakai, Tomoya Kono
Developmental and Comparative Immunology in press 2024.5
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)
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Comprehensive analysis of diel rhythmic expression of the medaka toll-like receptor gene family Reviewed
Hata T., Shimawaki H., Setoguchi S., Morimoto N., Hikima J.i., Sakai M., Kono T.
Developmental and Comparative Immunology 154 2024.5
Publishing type：Research paper (scientific journal) Publisher：Developmental and Comparative Immunology
Several immune-related genes, including Toll-like receptors (TLR), are associated with circadian rhythms in mammals. However, information on the circadian rhythmic expression of TLRs in fish is limited. In this study, we aimed to analyze the regulation of diel oscillations in the expression of TLR genes in Japanese medaka (Oryzias latipes). The expression analysis revealed diel expression patterns of tlr1, tlr5m, tlr21, and clock genes (bmal1 and clock1) under a 12 h light:12 h dark cycle. The clock gene response element (E-box) was identified in the transcriptional regulatory regions of tlr1, tlr5m, and tlr21. Moreover, overexpressed bmal1 and clock1 enhanced expression levels of tlr1, tlr5m, and tlr21 in medaka embryo (OLHdrR-e3) cells. The expression of tlr1, tlr5m, and tlr21 was significantly decreased in OLHdrR-e3 after generating a bmal1 knockdown using a morpholino oligo. These results indicate the regulation of the diel rhythmic expression of several fish TLRs by clock genes.
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Susceptibility f Japanese Medaka to Edwardsiella piscicida and E. anguillarum isolated from aquacultured fish. Reviewed
Aki Nishihara, Takechiyo Sumiyoshi, Koushirou Arakawa, Natsuki Morimoto, Shinya Yasumoto, Masakazu Kondo, Tomoya Kono, Masahiro Sakai, Jun-ichi Hikima
Fish Pathology 58 ( 4 ) 175 - 179 2023.12
Language：English Publishing type：Research paper (scientific journal)
Books 【 display / non-display 】
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月刊「養殖」サイトカインを用いた魚類免疫賦活剤の効果判定
酒井正博・高山博章・河野智哉（ Role： Joint author）
緑書房 2012.2
Responsible for pages：54-57 Language：Japanese
MISC 【 display / non-display 】
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Nishihara Aki, Sumiyoshi Takechiyo, Arakawa Koushiro, Morimoto Natsuki, Yasumoto Shinya, Kondo Masakazu, Kono Tomoya, Sakai Masahiro, Hikima Jun-ichi
Fish Pathology 58 ( 4 ) 175 - 179 2023
Language：English Publishing type：Rapid communication, short report, research note, etc. (scientific journal) Publisher：The Japanese Society of Fish Pathology
<i>Edwardsiella</i> bacteria infect Japanese medaka <i>Oryzias latipes</i>; however, the difference in susceptibility between <i>E. piscicida</i> and <i>E. anguillarum</i> is unknown. In this study, we performed challenge tests with medaka using four <i>Edwardsiella</i> strains, which were difficult to be identified by specific primers. The four strains were reclassified as three <i>E. piscicida</i> strains and one <i>E. anguillarum</i> strain using phylogenetic analysis. The immersion challenge demonstrated that medaka were more susceptible to <i>E. piscicida</i> than to <i>E. anguillarum</i>. Increases in the bacterial copy number in the infected medaka kidneys were correlated with the worsening of the external symptoms. Medaka is considered a valuable model organism for understanding the etiology of edwardsiellosis.
DOI： 10.3147/JSFP.58.175
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サイトカイン遺伝子を用いた魚類免疫賦活剤の効果判定
酒井正博、高山博章、河野智哉
養殖 2012.2
Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media) Publisher：緑書房
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Genomics of fish IL-17 ligand and receptors: A review.
T. Kono, H. Korenaga and M. Sakai
Fish and Shellfish Immunology ( 31 ) 635 - 643 2010.12
Language：English Publishing type：Article, review, commentary, editorial, etc. (scientific journal) Publisher：Elsevier
Presentations 【 display / non-display 】
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Edwardsiella piscicida由来Ivyの宿主リゾチームに対する阻害能
西原輝、住吉竹千代、安本信哉、近藤昌和、河野智哉、引間順一
令和6年度日本水産学会秋季大会 2024.9.26
Event date： 2024.9.24 - 2024.9.27
Presentation type：Oral presentation (general)
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時計遺伝子Bmal1変異メダカにおける免疫応答
羽田崇彦、平吉美憂、津田和忠、宮西弘、引間順一、河野智哉
令和6年度日本水産学会秋季大会 2024.9.26
Event date： 2024.9.24 - 2024.9.27
Presentation type：Oral presentation (general)
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メダカ組換えTNFの作製および制御性細胞死の誘導
中岡侑莉乃、石原怜於、西原輝、河野智哉、引間順一
令和6年度日本水産学会秋季大会 2024.9.26
Event date： 2024.9.24 - 2024.9.27
Presentation type：Oral presentation (general)
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魚類と病原体が織りなす感染の概日リズム
羽田崇彦、津田和忠、舩藏源、湯浅拓、引間順一、河野智哉
令和6年度日本魚病学会秋季大会
Event date： 2024.9.8 - 2024.9.9
Presentation type：Oral presentation (general)
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Edwardsiella piscicida由来病原因子EvpPによる宿主免疫応答の阻害
住吉竹千代、西原輝、安本信哉、近藤昌和、河野智哉、酒井正博、引間順一
令和6年度日本魚病学会秋季大会
Event date： 2024.9.8 - 2024.9.9
Presentation type：Oral presentation (general)
Awards 【 display / non-display 】
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農学特別賞 (研究部門)
2025.2 宮崎大学農学部
羽田崇彦、河野智哉
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2022年 宮崎大学学生農学特別賞表
2023.2 宮崎大学農学部 Diel rhythm of the inflammatory cytokine il1b in the Japanese medaka (Oryzias latipes) regulated by core components of the circadian clock
Tomoya Takeuchi, Takahiko Hata, Hiroshi Miyanishi, Takumi Yuasa, Suzuka Setoguchi, Ayaka Takeda, Natsuki Morimoto, Jun-ichi Hikima, Masahiro Sakai and Tomoya Kono
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日本水産学会 水産学進歩賞
2021.3 日本水産学会 魚類サイトカインによる自然免疫応答の概日リズム制御に関する研究
河野智哉
Award type：Award from Japanese society, conference, symposium, etc. Country：Japan
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宮崎大学農学部論文表彰
2018.1 宮崎大学農学部 Identification and immunoregulatory function of neuromedin U (Nmu) in the Japanese pufferfish Takifugu rubripes
河野智哉
Country：Japan
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北里大学同窓会研究奨励賞
2012.9 北里大学 魚類生理活性ペプチドのクロストークによる免疫制御機構の解析
河野智哉
Grant-in-Aid for Scientific Research 【 display / non-display 】
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魚類と病原体が織りなす感染の概日リズムの究明 ～感染症の防除を目指して～
Grant number：24K01837 2024.04 - 2028.03
独立行政法人日本学術振興会 科学研究費基金 基盤研究(B)
Authorship：Principal investigator
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生物時計が調節する魚類の免疫リズムの解明 ～感染症の防除を目指して～
Grant number：20H03064 2020.04 - 2024.03
独立行政法人日本学術振興会 科学研究費補助金 基盤研究(B)
Authorship：Principal investigator
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フグはなぜ疾病に強いのか？フグの耐病性のメカニズムを探る
Grant number：17H01486 2017.04 - 2021.03
科学研究費補助金 基盤研究(A)
Authorship：Coinvestigator(s)
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魚類の免疫応答における概日リズムの解明～免疫増強剤の効果向上を目指して～
Grant number：17K07915 2017.04 - 2020.03
科学研究費補助金 基盤研究(C)
Authorship：Principal investigator
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魚類免疫細胞をマーカーとした疾病診断法の確立
Grant number：26450264 2014.04 - 2017.03
科学研究費補助金 基盤研究(C)
Authorship：Principal investigator
魚類におけるヘルパーT(Th)細胞のサブセットを同定し、病原体感染時にこれらのThサブセットがどのような挙動を示すのかを解析し、新たな疾病診断技術の構築を目指すものである。
Available Technology 【 display / non-display 】
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魚類の免疫リズムの解明
魚病の防除を目的とした時間治療法の確立Home Page： 免疫生物学研究室
Related fields where technical consultation is available：・魚類免疫賦活剤の評価
・魚類の健康診断Message：・共同研究の希望テーマ：魚類の免疫力向上に関する研究