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Affiliation |
Faculty of Agriculture Region of Animal and Plant Biosciences |
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Professor |
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Laboratory Address |
農学部南棟6階 S617 |
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Laboratory Phone number |
0985-58-7507 |
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Homepage |
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External Link |
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Related SDGs |
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IGUCHI Atsushi
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Education 【 display / non-display 】
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Kobe University Graduate School, Division of National Science and Technology
- 2006.3
Country：Japan
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Kobe University Graduate School, Division of National Science and Technology
- 2003.3
Country：Japan
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Shiga University Faculty of Education
- 2000.3
Country：Japan
Campus Career 【 display / non-display 】
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University of Miyazaki Faculty of Agriculture Region of Animal and Plant Biosciences Professor
2025.04 - Now
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University of Miyazaki Faculty of Agriculture Department of Animal and Grassland Sciences Associate Professor
2014.04 - 2025.03
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University of Miyazaki Interdisciplinary Research Organization Assistant Professor
2010.03 - 2014.03
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University of Miyazaki Frontier Science Research Center Researcher
2006.04 - 2009.03
External Career 【 display / non-display 】
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Kobe University Assistant Professor
2009.4 - 2010.2
Professional Memberships 【 display / non-display 】
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日本獣医学会
2017.5
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日本臨床微生物学会
2011.10
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日本食品微生物学会
2010.4
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日本ゲノム微生物学会
2008.1
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日本細菌学会
2002.4
Papers 【 display / non-display 】
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Iguchi A., Ueno Y., Hoshinoo K., Okuno M., Uemura R., You G., Ogura Y., Takamatsu D.
Scientific Reports 15 ( 1 ) 2025.4
Authorship：Lead author, Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：Scientific Reports
The bovine respiratory disease complex (BRDC) is a global issue affecting dairy and beef farms and is of major concern due to the high morbidity and mortality rates in calves, as well as decreased production it causes, resulting in significant economic losses. Mannheimia haemolytica is one of the secondary pathogens associated with BRDC. M. haemolytica is classified into 12 serotypes based on capsular antigens. In addition to the prevalent serotypes A1, A2, and A6, strains belonging to other serotypes also cause respiratory diseases in cattle and other ruminants, necessitating a method for their rapid and easy identification. In this study, we organized the capsule biosynthesis genes based on genome information from all serotype strains and designed 11 PCR primer pairs targeting serotype-specific genes, which could individually identify serotypes A14/A16, which possess homologous genes, as well as all other serotypes. Additionally, we developed two multiplex PCR kits that include these serotype-specific and M. haemolytica species-specific primers. Specificity testing using reference strains confirmed that these kits can simultaneously and clearly identify both the species and their serotypes. The PCR-based system described here could be a valuable tool for subtyping M. haemolytica strains in epidemiological studies and surveillance efforts in cattle and other reservoir animals. This study also carefully compared and discussed the differences between the capsule synthesis genes of A8 and A14 from previously published and those obtained in this study.
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Infectious endophthalmitis associated with umbilical infection in Japanese black calf: a case report Reviewed International coauthorship
Sato R., Iguchi A., Uemura R., Tsujita H., Steiner A.
Frontiers in Veterinary Science 12 2025.4
Language：English Publishing type：Research paper (scientific journal) Publisher：Frontiers in Veterinary Science
A 3-day-old Japanese black calf presented with a swollen and tender umbilical cord and diffusely cloudy and keratoconus eyes. Abdominal ultrasonography confirmed mild enlargement of both umbilical arteries and the urachus with a hyperechoic lumen. Additionally, a hyperechogenic structure suggestive of pus was noted near the abdominal wall. Fluorescein staining revealed corneal epithelial injury, whereas slit lamp examination identified corneal edema, increased corneal thickness, and keratitis with vascularization of the corneal stroma. Based on these findings, diagnoses of omphaloarteritis, omphalourachitis, and bullous keratitis were made. Both umbilical arteries and the urachus were surgically removed; both ocular globes were covered with a third eyelid flap, which was released 30 days postoperatively. On the follow-up, ocular ultrasonography indicated bleeding or fibrin deposits in the vitreous body of the right ocular globe. Because intraocular inflammation was suspected, anterior aqueous humor was collected from the right ocular globe, and bacterial examination was performed with the umbilical artery abscess, urachal abscess, and intraabdominal pus collected intraoperatively. Escherichia coli was isolated from the umbilical artery abscess, urachal abscess, intraabdominal pus, and aqueous humor, and all isolates exhibited identical genotypes. These findings suggest that endophthalmitis occurred as a result of the hematogenous spread of bacteria originating from septic umbilical cord remnants and that ocular ultrasonography is useful for assessing intraocular pathologies.
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Elbastawesy A.M., Awasthi S.P., Hatanaka N., Hinenoya A., Iguchi A., Ombarak R.A., Deeb A.M.M., Yamasaki S.
International Dairy Journal 162 2025.3
Publishing type：Research paper (scientific journal) Publisher：International Dairy Journal
Milk and dairy products are popular in Egyptian diets, but their contamination with Escherichia coli, poses health risks. This study investigated the prevalence of potentially pathogenic and antimicrobial-resistant E. coli in raw milk and dairy products from Kafrelsheikh and Algarbia Governorates, Egypt. Two hundred ten samples including raw buffalo milk, goat milk, Domiati cheese, Domiati cheese with pepper, rayeb, and yogurt were analyzed. The prevalence of E. coli was 26.2%, with the highest occurrence in buffalo milk (68.0%) and the lowest in rayeb (7.5%). Based on ERIC-PCR, eighty-four non-clonal E. coli strains were selected and further characterized. Among tested virulence genes, adhesion genes such as lpfAO113 and ehaA, were the most prevalent. Toxin-encoding genes such as astA, cdt, cnf, and hlyA were also detected. The cytotoxic and hemolytic activity of cdt, cnf, and hylA carrying E. coli were confirmed on CHO cells and sheep blood agar, respectively. Twenty-three (27.4%) strains showed resistance to one or more antimicrobials, and 10 (11.9%) strains exhibited multidrug resistance (MDR). Among 12 antimicrobials tested resistance against ampicillin, streptomycin and tetracycline was the highest. Phylogenetic analysis and O-genotyping indicated clinically significant strains such as Og103, Og157 and OgGp9. Notably, two OgGp9 strains were OgGp9:Hg18 and phylogenetic group D, like those associated with a large diarrheal outbreak caused by milk consumption in Japan, in 2021. Interestingly, these two strains harbored a complete type 3 secretion system 2 locus (ETT2) and one of these strains was MDR. These findings indicate that these dairy products were contaminated with potentially pathogenic and multidrug-resistant E. coli. This is the first report to analyze E. coli contamination in Domiati cheese with pepper and detect OgGp9:Hg18 outbreak-associated strains with ETT2 and MDR in Egypt.
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<i>Escherichia coli</i> O-genotyping PCR and H-genotypingPCR Methods on Food Poisoning of Diarrheagenic <i>E. coli</i> Reviewed
Odagiri Masaaki, Kato Naoki, Sone Miki, Tsuchiya Akihiko, Kondo Takahide, Iguchi Atsushi
Japanese Journal of Food Microbiology 41 ( 3 ) 119 - 123 2024.9
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Japanese Society of Food Microbiology
DOI： 10.5803/jsfm.41.119
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Lee K., Iguchi A., Terano C., Hataya H., Isobe J., Seto K., Ishijima N., Akeda Y., Ohnishi M., Iyoda S.
Microbiology spectrum 12 ( 1 ) 2024.1
Publishing type：Research paper (scientific journal) Publisher：Microbiology spectrum
IMPORTANCE: Hemolytic uremic syndrome (HUS) is a life-threatening disease caused by Shiga toxin-producing Escherichia coli (STEC) infection. The treatment approaches for STEC-mediated typical HUS and atypical HUS differ, underscoring the importance of rapid and accurate diagnosis. However, specific detection methods for STECs other than major serogroups, such as O157, O26, and O111, are limited. This study focuses on the utility of PCR-based O-serotyping, serum agglutination tests utilizing antibodies against the identified Og type, and isolation techniques employing antibody-conjugated immunomagnetic beads for STEC isolation. By employing these methods, we successfully isolated a STEC strain of a minor serotype, O76:H7, from a HUS patient.
MISC 【 display / non-display 】
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大腸菌血清型別の"これまで"と"これから" Invited Reviewed
井口純
家畜感染症学会誌 12 ( 3 ) 73 - 84 2023.7
Authorship：Lead author, Corresponding author Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (scientific journal)
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腸管出血性大腸菌（EHEC）検査・診断マニュアル 2022年10月改訂 Invited
原田哲也、井口純、勢戸和子、伊豫田淳
国立感染症研究所 病原体検出マニュアル 2022.10
Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (other) Publisher：国立感染症研究所
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Le P.Q., Awasthi S.P., Hatanaka N., Hinenoya A., Hassan J., Ombarak R.A., Iguchi A., Tran N.T.T., Dao K.V.T., Vien M.Q., Le H.X., Do H.T., Yamamoto Y., Yamasaki S.
International Journal of Food Microbiology 370 2022.6
Publishing type：Rapid communication, short report, research note, etc. (scientific journal) Publisher：International Journal of Food Microbiology
The authors regret that the second affiliation of the sixth author was incorrectly written. The correct affiliation is Faculty of Veterinary Medicine, University of Sadat City, Sadat City, Egypt, as provided above. The authors would like to apologise for any inconvenience caused.
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腸管出血性大腸菌（EHEC）検査・診断マニュアル 2021年9月改訂 Invited
原田哲也、井口純、勢戸和子、伊豫田淳
国立感染症研究所 病原体検出マニュアル 2021.9
Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (other) Publisher：国立感染症研究所
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大腸菌の血清型別PCR法・続報（非定型O血清群の分類） Invited Reviewed
井口純
国立感染症研究所 病原微生物検出情報 42 94 - 95 2021.5
Authorship：Lead author, Corresponding author Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (bulletin of university, research institution) Publisher：国立感染症研究所
Presentations 【 display / non-display 】
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ベトナムにおける下痢症起因細菌のフィールド研究
井口純
長崎大学熱帯医学研究共同拠点成果報告 2024.11.28
Event date： 2024.11.28
Language：Japanese Presentation type：Oral presentation (general)
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Mannheimia haemolyticaの全血清型別PCR法の開発と実用性評価
井口純、福守調実、上村涼子、奥野未来、小椋義俊、星野尾歌織、上野勇一 、高松大輔
第167回日本獣医学会学術集会 2024.9.10
Event date： 2024.9.10 - 2024.9.11
Presentation type：Oral presentation (general)
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ウシ消化管内における大腸菌の空間的分布と多様性
井口純
第 20 回 21 世紀大腸菌研究会 2024.6.17
Event date： 2024.6.17 - 2024.6.18
Presentation type：Poster presentation
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E. coli Og-typing PCRによる便培養液中の大腸菌構成解析
岩田 佳音、伊豫田 淳、井口 純
第44回日本食品微生物学会学術総会 2023.9.21
Event date： 2023.9.21 - 2023.9.22
Presentation type：Oral presentation (general)
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ニホンジカやキョンなどの野生動物が保有する病原性大腸菌の調査
中塚 瑞涼、加藤 結子、伊豫田 淳、井口 純
第44回日本食品微生物学会学術総会 2023.9.21
Event date： 2023.9.21 - 2023.9.22
Presentation type：Oral presentation (general)
Industrial property rights 【 display / non-display 】
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大腸菌非定型O血清群のO抗原コード遺伝子を標的としたPCR分類法
井口 純,加藤 結子
Applicant：宮崎大学
Application no：2021-106157 Date applied：2021.6.25
Announcement no：特開2023-004478 Date announced：2023.1.17
Country of applicant：Domestic
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PCR法を用いた赤痢菌の広域O血清群判定方法
井口 純, 西井 啓修
Applicant：宮崎大学
Application no：2021-501840 Date applied：2020.2.5
Announcement no：WO2020/170823 Date announced：2020.8.27
Patent/Registration no：7479640 Date registered：2024.4.26
Country of applicant：Domestic , International (PCT) application Country of acquisition：Domestic
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PCR法を用いた大腸菌の広域鞭毛抗原型判定方法
井口純, 番上将也
Applicant：国立大学法人宮崎大学
Application no：PCT/JP2017/25817 Date applied：2017.7.14
Announcement no：WO2018/016451 Date announced：2018.1.25
Country of applicant：Domestic
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PCR法を用いた大腸菌の広域O血清群判定方法
井口 純
Applicant：宮崎大学
Application no：2015-508754 Date applied：2014.3.28
Announcement no：WO2014/157611 Date announced：2014.10.2
Patent/Registration no：6500773 Date registered：2019.3.29
Country of applicant：Domestic
Awards 【 display / non-display 】
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宮崎大学ハイステップ表彰
2025.3 宮崎大学 Genome evolution and plasticity of serratia marcescens, an important multidrugresistant nosocomial pathogen
井口 純
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地域貢献活動表彰・優秀賞
2024.12 宮崎大学農学部 食中毒の予防と制御に資する国内外での検査・研究支援と啓蒙活動
井口 純
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宮崎大学ハイステップ表彰
2024.3 宮崎大学
井口純
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令和3度宮崎大学農学部論文表彰 最優秀論文賞
2021.9 宮崎大学農学部 Additional Og-Typing PCR Techniques Targeting Escherichia coli-Novel and Shigella-Unique O-Antigen Biosynthesis Gene Clusters
井口純
Country：Japan
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平成30年度宮崎大学農学部論文表彰 最優秀論文賞
2018.12 宮崎大学農学部 Escherichia coli H-Genotyping PCR: a Complete and Practical Platform for Molecular H Typing
井口純
Country：Japan
Grant-in-Aid for Scientific Research 【 display / non-display 】
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ウシ消化管内で共生するヒト病原細菌の空間・生態的特徴の解明と新たな防除法の構築
Grant number：23K27059 2023.04 - 2026.03
独立行政法人日本学術振興会 科学研究費基金 基盤研究(B)
Authorship：Principal investigator
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ウシ消化管内で共生するヒト病原細菌の空間・生態的特徴の解明と新たな防除法の構築
Grant number：23H02366 2023.04 - 2026.03
独立行政法人日本学術振興会 科学研究費補助金 基盤研究(B)
Authorship：Principal investigator
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大腸菌抗原変化のメカニズム解明と多様化した糖鎖抗原の機能について
Grant number：16K08780 2016.04 - 2019.03
科学研究費補助金 基盤研究(C)
Authorship：Principal investigator
大腸菌の菌体表層には糖鎖抗原（O抗原）が発現しており、その抗原性の種類は180を超えている。本研究ではO抗原の多様性に注目し、①抗原変化の遺伝学的な組換えメカニズムを解明する。さらに②多様化した糖鎖構造の宿主に対する機能性（生存性や病原性）を検証する。
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カンピロバクター食中毒の発生に寄与する二次汚染要因の探索
Grant number：16K00941 2016.04 - 2019.03
科学研究費補助金 基盤研究(C)
Authorship：Coinvestigator(s)
カンピロバクター食中毒の発生に寄与する二次汚染要因の探索
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新規O血清群に属する志賀毒素産生性大腸菌のゲノム構造と病原因子の解析
Grant number：25460540 2013.04 - 2016.03
科学研究費補助金 基盤研究(C)
Authorship：Principal investigator
大腸菌の新規O血清群に属する志賀毒素産生性大腸菌（Shiga-toxin producing E. coli：STEC）について、ゲノム情報を基盤とした進化系統解析と病原因子の解析を行い、新興STECとしての特徴を理解するとともに、今後の感染症対策に役立てる。
Available Technology 【 display / non-display 】
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家畜・家禽・野生動物から分離される細菌の研究
細菌のゲノム解析とその情報を用いた新規検査法の開発
衛生教育に使用する教材等の作製Related fields where technical consultation is available：細菌学、ゲノム科学
Message：研究内容やこれまでの業績などの詳細は、研究室HPでご確認ください
Committee Memberships 【 display / non-display 】
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腸管出血性大腸菌研究会 運営委員
2024.4
Committee type：学協会
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家畜感染症学会 評議員
2024.4
Committee type：学協会
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日本食品微生物学会 評議員
2020.1
Committee type：学協会
Social Activities 【 display / non-display 】
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CADIC特別セミナー「Treasure every meeting〜アフリカで繋がった感染症研究、そしてベトナムのコウモリ由来感染症フィールド調査〜」
Role(s)： Presenter, Planner
2025.2.28
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第3回下痢原性大腸菌等遺伝子検査法講習会
Role(s)： Lecturer
宮崎大学農学部 2024.8.22 - 2024.8.23
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第2回下痢原性大腸菌等遺伝子検査法講習会
Role(s)： Lecturer
宮崎大学農学部 2024.7.11 - 2024.7.12
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宮崎県立高鍋農業高校・農業クラブ活動の指導（菌床焼却灰の殺菌効果の検証）
Role(s)： Advisor
2024.6.5 - 2024.10.10
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CADIC特別セミナー「グローバルヘルスの課題解決に挑む」
Role(s)： Presenter, Planner
2023.11.30