論文 - 井口 純
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Arimizu Y, Kirino Y, Sato MP, Uno K, Sato T, Gotoh Y, Auvray F, Brugere H, Oswald E, Mainil JG, Anklam KS, Döpfer D, Yoshino S, Ooka T, Tanizawa Y, Nakamura Y, Iguchi A, Morita-Ishihara T, Ohnishi M, Akashi K, Hayashi T, Ogura Y.
Genome Research 29 ( 9 ) 1495 - 1505 2019年9月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Escherichia coli H-Genotyping PCR: a Complete and Practical Platform for Molecular H Typing 査読あり
Banjo M, Iguchi A, Seto K, Kikuchi T, Harada T, Scheutz F, Iyoda S
Journal of Clinical Microbiology 56 ( 6 ) 2018年5月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Thiry D., De Rauw K., Takaki S., Duprez J., Iguchi A., Piérard D., Korsak N., Mainil J.
Journal of Applied Microbiology 124 ( 3 ) 867 - 873 2018年3月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Journal of Applied Microbiology
© 2017 The Society for Applied Microbiology Aims: The purpose of this survey was to estimate the respective prevalence of the ‘gang of seven’ and ‘non-gang of seven’ serotypes of Shigatoxigenic and enteropathogenic Escherichia coli and to identify the O80:H2 serotype in 245 intestinal contents collected at two slaughterhouses in Belgium in 2014. Methods and Results: After overnight enrichment growth, the 69 intestinal contents testing positive with PCR targeting the eae, stx1 and stx2 genes were inoculated onto four agar media. Of the 2542 colonies picked up, 677 from 59 samples were PCR confirmed. The most frequent virulotypes were eae+ in 47 (80%) samples, stx2+ in 20 (34%) samples and eae+ stx1+ in 16 (27%) samples. PCR-positive colonies belonged to different virulotypes in 36 samples. No colony was O80-positive, whereas two eae+ colonies from two samples were O26:H11, 50 eae+ stx1+ and eae+ from eight samples were O103:H2 and two eae+ stx1+ stx2+ colonies from one sample were O157:H7. Conclusions: The ‘non-gang of seven’ serotypes are more frequent than the ‘gang of seven’ serotypes and the O80:H2 serotype was not detected among Shigatoxigenic and enteropathogenic Escherichia coli in the intestines of cattle at these two slaughterhouses. Significance and Impact of the Study: Although the identification protocols of Shigatoxigenic Escherichia coli focus on the ‘gang of seven’ serotypes, several other serotypes can be present with possible importance in public health. Innovative selective identification procedures should be designed.
DOI： 10.1111/jam.13677
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Bacterial flora analysis of coliforms in sewage, river water, and ground water using MALDI-TOF mass spectrometry 査読あり
Suzuki Y, Niina K, Matsuwaki T, Nukazawa K, Iguchi A
Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering 53 ( 2 ) 160 - 173 2018年1月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Comparison of Three Molecular Subtyping Methods among O157 and Non-O157 Shiga Toxin-Producing Escherichia coli Isolates from Japanese Cattle 査読あり
Nakamura H, Iguchi A, Maehara T, Fujiwara K, Fujiwara A, Ogasawara J
Japanese Journal of Infectious Diseases 7 ( 1 ) 45 - 50 2018年1月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Enteropathogenic Escherichia coli O80:H2 in Young Calves with Diarrhea, Belgium 査読あり
Thiry D, Saulmont M, Takaki S, De Rauw K, Duprez JN, Iguchi A, Piérard D, Mainil JG
Emerging Infectious Diseases 23 ( 12 ) 2093 - 2095 2017年12月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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An untypeable enterotoxigenic Escherichia coli represents one of the dominant types causing human disease 査読あり
Iguchi A, von Mentzer A, Kikuchi T, Thomson NR
Microbial Genomics 3 ( 9 ) 2017年9月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Shiga Toxin Subtypes and Virulence Genes in Escherichia coli Isolated from Cattle 査読あり
Akiyama Yumi, Futai Hiroko, Saito Etsuko, Ogita Kenichi, Sakae Hiroshi, Fukunaga Masaharu, Tsuji Hidetaka, Chikahira Masatsugu, Iguchi Atsushi
Japanese Journal of Infectious Diseases 70 ( 2 ) 181 - 185 2017年5月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：国立感染症研究所 Japanese Journal of Infectious Diseases 編集委員会
Subtypes of <i>stx1</i> and <i>stx2</i> in 45 Shiga toxin-producing <i>Escherichia coli</i> (STEC) strains isolated from cattle were investigated by PCR. Only subtype <i>stx1a</i> was detected among all the <i>stx1</i>-positive strains. The major <i>stx2</i> subtype was <i>stx2a</i> followed by <i>stx2d</i>, <i>stx2c</i>, <i>stx2b</i>, and <i>stx2g</i> in decreasing order of frequency. <i>stx2c</i> was found in strains of serotypes O157 and O174. <i>stx2d</i> was found in 11 strains. These strains were confirmed by DNA sequencing to carry both the activatable tail and the END motif; all were <i>eae</i>-negative, and 3 contained <i>stx2d</i> as the only <i>stx</i>. <i>stx2g</i> was found in 2 strains in association with <i>stx2a</i>, <i>estA1</i>, and <i>astA</i>. In addition, 7 hybrid strains of shigatoxigenic and enterotoxigenic <i>E. coli</i> (STEC/ETEC) were found to harbor one or both of <i>stx1a</i> and <i>stx2a</i> (<i>stx1a</i>/<i>stx2a</i>) and <i>estA1</i>. Among 27 serotypes of STEC strains isolated from cattle, O157:H7 and O109:H– strains were <i>eae</i>-positive. Other putative adhesin genes, such as <i>saa</i>, <i>iha</i>, <i>espP</i>, and <i>lpfA</i><sub>O113</sub> were detected in more than 12 serotypes.
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Characterization of Shiga toxin-producing Escherichia coli from feces of sika deer (Cervus nippon) in Japan using PCR binary typing analysis to evaluate their potential human pathogenicity. 査読あり
Kabeya H, Sato S, Oda S, Kawamura M, Nagasaka M, Kuranaga M, Yokoyama E, Hirai S, Iguchi A, Ishihara T, Kuroki T, Morita-Ishihara T, Iyoda, Terajima J, Ohnishi M, Maruyama S
Journal of Veterinary Medical Science 79 ( 5 ) 834 - 841 2017年5月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Identification of Shiga toxin-producing (STEC) and enteropathogenic (EPEC) Escherichia coli in diarrhoeic calves and comparative genomics of O5 bovine and human STEC. 査読あり
Fakih I, Thiry D, Duprez JN, Saulmont M, Iguchi A, Piérard D, Jouant L, Daube G, Ogura Y, Hayashi T, Taminiau B, Mainil JG
Veterinary Microbiology 2017年4月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Identification of a New Virulent Clade in Enterohemorrhagic Escherichia coli O26:H11/H- Sequence Type 29 査読あり
Nozomi Ishijima, Ken-ichi Lee, Tomomi Kuwahara、Haruyuki Nakayama-Imaohji、Saori Yoneda、Atsushi Iguchi、Yoshitoshi Ogura、Tetsuya Hayashi、Makoto Ohnishi、Sunao Iyoda
Scientific Reports 2017年2月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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大腸菌のO抗原合成遺伝子領域の解析と検査法の確立 招待あり 査読あり
井口 純
日本細菌学雑誌 71 ( 4 ) 209 - 215 2016年12月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：日本細菌学会
大腸菌はO血清群を指標として細分類され, 特に病原性大腸菌の調査や研究においては, 分離株の優先すべき特徴としてO血清群の判定が行われる。O血清群の多様性はO抗原合成遺伝子群の多様性と関連しており, それぞれのO抗原合成遺伝子群に特徴的な塩基配列はO血清群の判定に利用できる。我々はこれまでに定められているO1からO187までのすべてのO血清群におけるO抗原合成遺伝子群の配列を決定し, 保存性と特異性の両面における特徴を明らかにした。さらにその解析結果を基に, O血清群を網羅的に判定できる2つの遺伝学的な手法を開発した。一つはPCRを基礎とするマルチプレックスPCR法（<i>E. coli</i> O-genotyping PCR）で, もう一つは<i>wzx/wzy</i>と<i>wzm/wzt</i>の配列セットを用いたBLAST検索による方法（SerotypeFinder）である。いずれの方法で得られる結果も表現型に準じており, 今後, 大腸菌における調査や研究において幅広い利用が期待される。
DOI： 10.3412/jsb.71.209
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Secondary Shiga toxin–producing Escherichia coli infection, Japan, 2010–2012 査読あり
Morita-Ishihara T., Iyoda S., Iguchi A., Ohnishi M.
Emerging Infectious Diseases 22 ( 12 ) 2181 - 2184 2016年12月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Emerging Infectious Diseases
© 2016, Centers for Disease Control and Prevention (CDC). All rights reserved. To evaluate the potential public health risk caused by secondary Shiga toxin–producing Escherichia coli (STEC) infections in Japan, we investigated the prevalence and characteristics of STEC isolated from healthy adults during 2010–2012. Although prevalence among healthy adults was high, most STEC organisms displayed characteristics rarely found in isolates from symptomatic patients.
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Phylogenetic Analysis of Enteroaggregative Escherichia coli (EAEC) Isolates from Japan Reveals Emergence of CTX-M-14-Producing EAEC O25:H4 Clones Related to Sequence Type 131. 査読あり
Naoko Imuta, Tadasuke Ooka, Kazuko Seto, Ryuji Kawahara, Toyoyasu Koriyama, Tsuyoshi Kojyo, Atsushi Iguchi, Koichi Tokuda, Hideki Kawamura, Kiyotaka Yoshiie, Yoshitoshi Ogura, Tetsuya Hayashi, Junichiro Nishia
Journal of Clinical Microbiology 2016年8月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Six novel O genotypes from Shiga toxin-producing Escherichia coli 査読あり
Iguchi A., Iyoda S., Seto K., Nishii H., Ohnishi M., Mekata H., Ogura Y., Hayashi T.
Frontiers in Microbiology 7 ( MAY ) 2016年5月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Frontiers in Microbiology
© 2016 Iguchi, Iyoda, Seto, Nishii, Ohnishi, Mekata, Ogura and Hayashi. Serotyping is one of the typing techniques used to classify strains within the same species. O-serogroup diversification shows a strong association with the genetic diversity of O-antigen biosynthesis genes. In a previous study, based on the O-antigen biosynthesis gene cluster (O-AGC) sequences of 184 known Escherichia coli O serogroups (from O1 to O187), we developed a comprehensive and practical molecular O serogrouping (O genotyping) platform using a polymerase chain reaction (PCR) method, named E. coli O-genotyping PCR. Although, the validation assay using the PCR system showed that most of the tested strains were successfully classified into one of the O genotypes, it was impossible to classify 6.1% (35/575) of the strains, suggesting the presence of novel O genotypes. In this study, we conducted sequence analysis of O-AGCs from O-genotype untypeable Shiga toxin-producing E. coli (STEC) strains and identified six novel O genotypes; OgN1, OgN8, OgN9, OgN10, OgN12 and OgN31, with unique wzx and/or wzy O-antigen processing gene sequences. Additionally, to identify these novel O-genotypes, we designed specific PCR primers. A screen of O genotypes using O-genotype untypeable strains showed 13 STEC strains were classified into five novel O genotypes. The O genotyping at the molecular level of the O-AGC would aid in the characterization of E. coli isolates and will assist future studies in STEC epidemiology and phylogeny.
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Prevalence and pathogenic potential of Escherichia coli isolates from raw milk and raw milk cheese in Egypt 査読あり
Ombarak RA, Hinenoya A, Awasthi SP, Iguchi A, Shima A, Elbagory AR, Yamasaki S
International Journal of Food Microbiology 2016年3月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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さまざまなO血清群に属する志賀毒素産生性大腸菌の市販選択培地上での生育特性 査読あり
秋吉充子、中村寛海、伊豫田淳、石原朋子、加藤結子、井口純
日本食品微生物学会雑誌 2015年12月
記述言語：日本語 掲載種別：研究論文（学術雑誌）
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腸管出血性大腸菌の主要なO血清群と病原性遺伝子を 判定するOne-shotマルチプレックスPCR法の開発と評価 査読あり
井口 純、秋 吉 充 子、伊豫田淳、大 西真
日本食品微生物学会雑誌 2015年12月
記述言語：日本語 掲載種別：研究論文（学術雑誌）
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Multiplex Real-Time PCR Assays for Screening of Shiga Toxin 1 and 2 Genes, Including All Known Subtypes, and Escherichia coli O26-, O111-, and O157-Specific Genes in Beef and Sprout Enrichment Cultures 査読あり
Harada T, Iguchi A, Iyoda S, Seto K, Taguchi M, Kumeda Y
Journal of Food Protection 2015年10月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Joensen K., Tetzschner A., Iguchi A., Aarestrup F., Scheutz F.
Journal of Clinical Microbiology 53 ( 8 ) 2410 - 2426 2015年8月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Journal of Clinical Microbiology
Copyright © 2015, American Society for Microbiology. All Rights Reserved. Accurate and rapid typing of pathogens is essential for effective surveillance and outbreak detection. Conventional serotyping of Escherichia coli is a delicate, laborious, time-consuming, and expensive procedure. With whole-genome sequencing (WGS) becoming cheaper, it has vast potential in routine typing and surveillance. The aim of this study was to establish a valid and publicly available tool for WGS-based in silico serotyping of E. coli applicable for routine typing and surveillance. A FASTA database of specific O-antigen processing system genes for O typing and flagellin genes for H typing was created as a component of the publicly available Web tools hosted by the Center for Genomic Epidemiology (CGE) (www.genomicepidemiology.org). All E. coli isolates available with WGS data and conventional serotype information were subjected to WGS-based serotyping employing this specific SerotypeFinder CGE tool. SerotypeFinder was evaluated on 682 E. coli genomes, 108 of which were sequenced for this study, where both the whole genome and the serotype were available. In total, 601 and 509 isolates were included for O and H typing, respectively. The O-antigen genes wzx, wzy, wzm, and wzt and the flagellin genes fliC, flkA, fllA, flmA, and flnA were detected in 569 and 508 genome sequences, respectively. SerotypeFinder for WGS-based O and H typing predicted 560 of 569 O types and 504 of 508 H types, consistent with conventional serotyping. In combination with other available WGS typing tools, E. coli serotyping can be performed solely from WGS data, providing faster and cheaper typing than current routine procedures and making WGS typing a superior alternative to conventional typing strategies.
DOI： 10.1128/JCM.00008-15