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Affiliation |
Faculty of Medicine School of Medicine Department of Anatomy, Ultrastructural Cell Biology |
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Associate Professor |
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External Link |
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TAKAHASHI Nobuyasu
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Papers 【 display / non-display 】
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Imazato H., Takahashi N., Hirakawa Y., Yamaguchi Y., Hiyoshi M., Tajima T., Chosa E., Sawaguchi A.
Scientific Reports 13 ( 1 ) 6352 2023.12
Language：English Publishing type：Research paper (scientific journal) Publisher：Scientific Reports
Recent physiological studies have shown that the deep fascia has received much attention concerning clinical medicine; however, histological examination of the deep fascia has not been well established. In this study, we aimed to clarify and visualize the structure of the deep fascia by taking advantage of cryofixation techniques and low-vacuum scanning electron microscopy. As a result, the ultrastructural observations revealed three-dimensional stratification of the deep fascia composed of three layers: the first superficial layer consisting of collagen fibers extending in various directions with blood vessels and peripheral nerves; the second intermediate layer formed by single straight and thick collagen fibers with flexibility; and the third deepest layer, consisting of relatively straight and thin collagen fibers. We explored the use of two hooks to hold a piece of deep fascia in place through the course of cryo-fixation. A comparative observation with or without the hook-holding procedure would indicate the morphological adaptation to physiological stretch and contraction of the deep fascia. The present morphological approach paves the way to visualize three-dimensional ultrastructures for future biomedical studies including clinical pathophysiology.
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Insertion sites of the muscles attached to the clavicle: a cadaveric study of the clavicle Reviewed
Imazato H., Takahashi N., Sawaguchi A., Hirakawa Y., Yamaguchi Y., Hiyoshi M., Tajima T., Chosa E.
BMC Musculoskeletal Disorders 24 ( 1 ) 160 2023.12
Language：English Publishing type：Research paper (scientific journal) Publisher：BMC Musculoskeletal Disorders
Background: Clavicle fractures are common injuries, especially in young, active individuals. Operative treatment is recommended for completely displaced clavicle shaft fractures, and plate fixation is stronger than the use of intramedullary nails. Few studies have reported on iatrogenic injuries to the muscle attached to the clavicle during fracture surgery. The aim of this study was to clarify the area of the insertion sites of muscles attached to the clavicle in Japanese cadavers using gross anatomy and three-dimensional (3D) analysis. We also aimed to compare the effects of anterior plate templating and superior plate templating on clavicle shaft fractures using 3D images. Methods: Thirty-eight clavicles from Japanese cadavers were analyzed. We removed all clavicles to identify the insertion sites and measured the size of the insertion area of each muscle. Three-dimensional templating was performed on both the superior and anterior plates of the clavicle using data obtained from computed tomography. The areas covered by these plates on the muscles attached to the clavicle were compared. Histological examination was performed on four randomly selected specimens. Results: The sternocleidomastoid muscle was attached proximally and superiorly; the trapezius muscle was attached posteriorly and partly superiorly; and the pectoralis major muscle and deltoid muscles were attached anteriorly and partially superiorly. The non-attachment area was located mainly in the posterosuperior part of the clavicle. It was difficult to distinguish the borders of the periosteum and pectoralis major muscles. The anterior plate covered a significantly broader area (mean 6.94 ± 1.36 cm2) of the muscles attached to the clavicle than did the superior plate (mean 4.11 ± 1.52 cm2) (p < 0.0001). On microscopy, these muscles were inserted directly into the periosteum. Conclusion: Most of the pectoralis major and deltoid muscles were attached anteriorly. The non-attachment area was located mainly from the superior to posterior part of the clavicle midshaft. Both macroscopically and microscopically, the boundaries between the periosteum and these muscles were difficult to demarcate. The anterior plate covered a significantly broader area of the muscles attached to the clavicle than that by the superior plate.
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A Suggested Mechanism for Green Discoloration of the Postmortem Brain Reviewed
Shinkawa N., Takahashi N., Yano K., Sawaguchi A., Sonoda A., Kakizaki E., Yukawa N.
American Journal of Forensic Medicine and Pathology 44 ( 2 ) 132 - 135 2023.6
Language：English Publishing type：Research paper (scientific journal) Publisher：American Journal of Forensic Medicine and Pathology
In the putrefied brain, the cortex and basal ganglia show dark-grayish to green discoloration due to sulfhemoglobin formed from hydrogen sulfide (H2S) produced by endogenous bacteria and hemoglobin. In this study, we propose and demonstrate another mechanism of green discoloration in the brain. The formalin-fixed brain of a cadaver donated for medical education with no putrefaction was used. Half of the brain was immersed in sodium hydrosulfide solution, to imitate the H2S produced by bacteria. This half showed greenish discoloration, mainly in the basal ganglia and cortex. The other half showed positive Perls' Prussian blue staining, mainly in the basal ganglia and cortex. The area of greenish discoloration due to H2S and the region positive for Perls' Prussian blue staining coincided. Tissue treatment with strong oxidizing agents is required to liberate heme iron. The positive Perls' Prussian blue staining in this study thus does not reflect heme iron. In conclusion, we considered that non-heme iron compounds physiologically present in the brain and H2S represent sources of putrefactive greenish discoloration in the brain.
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In situ strategy for biomedical target localization via nanogold nucleation and secondary growth
Sawaguchi A., Kamimura T., Takahashi N., Yamashita A., Asada Y., Imazato H., Aoyama F., Wakui A., Sato T., Choijookhuu N., Hishikawa Y.
Communications Biology 4 ( 1 ) 710 2021.12
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Communications Biology
Immunocytochemistry visualizes the exact spatial location of target molecules. The most common strategy for ultrastructural immunocytochemistry is the conjugation of nanogold particles to antibodies as probes. However, conventional nanogold labelling requires time-consuming nanogold probe preparation and ultrathin sectioning of cell/tissue samples. Here, we introduce an in situ strategy involving nanogold nucleation in immunoenzymatic products on universal paraffin/cryostat sections and provide unique insight into nanogold development under hot-humid air conditions. Nanogold particles were specifically localized on kidney podocytes to target synaptopodin. Transmission electron microscopy revealed secondary growth and self-assembly that could be experimentally controlled by bovine serum albumin stabilization and phosphate-buffered saline acceleration. Valuable retrospective nanogold labelling for gastric H+/K+-ATPase was achieved on vintage immunoenzymatic deposits after a long lapse of 15 years (i.e., 15-year-old deposits). The present in situ nanogold labelling is anticipated to fill the gap between light and electron microscopy to correlate cell/tissue structure and function.
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Takahashi N, Aoyama F, Sawaguchi A
Microscopy (Oxford, England) 70 ( 2 ) 192 - 200 2020.8
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Microscopy
Mini-abstract: Application of a three-dimensional culture system with air exposure facilitates the formation of large cell spheres possessing cribriform glands and producing mucin in the collagen gel. Transmission electron microscopy revealed the formation of microvilli and junctional complexes at the apical side of the cell. This study aimed to reproduce the characteristics of original adenocarcinoma tumors in vitro. The pancreatic cell line, SUIT-58, derived from a moderately differentiated adenocarcinoma of metastatic pancreatic cancer was used. The cells have a sheet structure in conventional cell culture without forming glands or exhibiting mucin production in the lumen. First, the necessity of scaffolds to create an adenocarcinoma-like microenvironment for SUIT-58 pancreatic cancer cells was assessed. Compared with conventional culture plates, the use of type I collagen as a scaffold played an important role in the formation of densely congested microvilli, as observed through scanning electron microscopy. As gland formation is one of the features of adenocarcinoma, we also assessed gland formation. Use of a recently developed three-dimensional culture system with air exposure resulted in the formation of large cell spheres possessing cribriform glands, which released mucin into the lumen. Transmission electron microscopy also revealed the formation of microvilli in the lumen of the glands and junctional complex at the intercellular part, which were similar to those observed in xenografts. These findings indicate that an in vitro three-dimensional culture system with air exposure reflects the intrinsic features of the original tumor, suggesting that this culture system could be useful for preliminary research of certain cancers.
MISC 【 display / non-display 】
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Primary pulmonary angiosarcoma: A case report.
Hiroyuki Tanaka, Kenji Yorita, Nobuyasu Takahashi, Yasuhiro Usuma, Kunihide Nakamura and Hiroaki Kataoka
Pathology international 65 ( 10 ) 554 - 557 2015.8
Language：English Publishing type：Research paper, summary (national, other academic conference) Publisher：WILEY-BLACKWELL
DOI： 10.1111/pin.12341
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Synchronous solid pseudopapillary neoplasm and intraductal papillary mucinous neoplasm of the pancreas: report of a case.
"NAOYA IMAMURA, KAZUO CHIJIIWA, JIRO OHUCHIDA, MASAHIDE HIYOSHI, NOBUYASU TAKAHASHI, KENJI YORITA,
Surgery Today 41 ( 6 ) 865 - 871 2011.6
Language：English Publishing type：Article, review, commentary, editorial, etc. (scientific journal) Publisher：Springer
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Enlarged solitary necrotic nodule of the liver misinterpreted （共著）
Otani K, Chijiiwa K, Kondo K, Tsuchimochi M, Takahashi N, Ikenaga N, Ohuchida J,
Clinical Journal of Gastroenterology 2 ( 5 ) 355 - 360 2009.5
Language：English Publishing type：Article, review, commentary, editorial, etc. (scientific journal) Publisher：Clinical Journal of Gastroenterology
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外傷性肝細胞癌破裂に対し肝動脈塞栓術を施行後切除した1例（共著）
新名一郎, 千々岩一男, 近藤千博, 永野元章, 内山周一郎, 高橋伸育, 片岡寛章
日本消化器外科学会雑誌 2008.9
Language：Japanese Publishing type：Research paper, summary (national, other academic conference) Publisher：日本消化器外科学会雑誌
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Activation of MET receptor tyrosine kinase in ulcer surface epithelial cells undergoing restitution
Nagai M., Takahashi N., Miyazawa K., Kawaguchi M., Chijiiwa K., Kataoka H.
Pathology International 58 ( 7 ) 462 - 464 2008.7
Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (bulletin of university, research institution) Publisher：Pathology International
Presentations 【 display / non-display 】
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グリオキサール系・ホルマリン代替固定液・ALTFixⓇを用いた灌流固定ラットの早期における固定性の評価
豊嶋（青山）典世、高橋伸育、澤口 朗
第125回日本解剖学会総会全国学術集会
Event date： 2020.3.25 - 2020.3.27
Language：Japanese Presentation type：Oral presentation (general)
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光顕用切片の標的物質局在をナノスケールで可視化する新たな免疫電顕解析技術と再生臓器開発・評価への応用
澤口 朗、上村 健、菱川善隆、山下 篤、豊嶋典世、髙橋伸育、今里浩之、浅田祐士郎
第19回日本再生医療学会総会
Event date： 2020.3.12 - 2020.3.14
Language：Japanese Presentation type：Poster presentation
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グリオキサール系・ホルマリン代替固定液・ALTFixRを用いたラット脳を中心とする固定性の評価
豊嶋（青山）典世、高橋伸育、澤口 朗
第75回日本解剖学会九州支部学術集会
Event date： 2019.11.9
Language：Japanese Presentation type：Oral presentation (general)
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気相―液層界面法を用いた膵癌細胞株SUIT-2の三次元培養と電顕観察
高橋伸育、青山典世、澤口 朗
第75回日本顕微鏡学会 学術講演会
Event date： 2019.6.17 - 2019.6.19
Language：Japanese Presentation type：Poster presentation
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再生臓器開発とその臨床応用に資する簡便な電顕立体解析手法の開発と大容量画像共有クラウドシステムの応用
澤口 朗、高橋伸育、豊嶋典世
第18回日本再生医療学会総会
Event date： 2019.3.21 - 2019.3.23
Language：Japanese Presentation type：Oral presentation (general)
Grant-in-Aid for Scientific Research 【 display / non-display 】
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肝様腺癌の病態解明と新規抗体による治療戦略の開発
Grant number：18K08686 2019.04 - 2021.03
科学研究費補助金 基盤研究(C)
胃癌の特殊型である肝様腺癌は血清AFPの上昇を伴い、通常の胃癌と比較して高率に脈管侵襲や肝転移を起こし、薬剤への抵抗性を示す予後不良の疾患である。その報告は年々増加する一方、生物学的特性については不明な点が多く、研究に有用なツールも不十分である。研究代表者は十二指腸乳頭部を由来とする新規肝様腺癌細胞株の樹立に成功しており、これを用いて浸潤、転移に関わる因子を同定し、有効な治療戦略を確立することが本研究の目的である。Glypican-3 (GPC3)は肝細胞癌に特徴的なマーカーであり、予後不良因子として報告されている。この遺伝子に対する新規抗体gc33を用いた実験では抗体依存性細胞性細胞障害作用を賦活させることにより抗腫瘍効果があることが報告されており、抗体 gc33を肝細胞癌と類似した性質を持つ肝様腺癌に応用することで、予後不良な肝様腺癌の治療法にブレークスルーをもたらすことが期待される。
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肝様腺癌に対する新規抗体を用いた治療戦略
2014.04 - 2017.03
科学研究費補助金 基盤研究(C)
Authorship：Principal investigator
肝様腺癌に対する新規抗体を用いた治療戦略
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膵癌の浸潤、転移に関与するＷｎｔ関連遺伝子の機能解析
2011.04 - 2013.03
科学研究費補助金 若手研究(B)
Authorship：Principal investigator
膵癌の浸潤、転移に関与するＷｎｔ関連遺伝子の機能解析