SONODA Hiroko

写真a

Affiliation

Faculty of Agriculture Department of Veterinary Science

Title

Associate Professor

External Link

Degree 【 display / non-display

  • 博士(獣医学) ( 2010.3   山口大学 )

Research Areas 【 display / non-display

  • Life Science / Veterinary medical science

 

Papers 【 display / non-display

  • Upregulation of NADPH Oxidase 2 Contributes to Renal Fibrosis in pcy Mice: An Experimental Model of Nephronophthisis Reviewed

    Hoshino Y., Sonoda H., Mikoda N., Ikeda M.

    Nephron   1 - 11   2021.12

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Nephron  

    Background: DBA/2FG-pcy (pcy) mice harbor a homozygous Nphp3 missense mutation and develop nephronophthisis with renal interstitial fibrosis. Previous studies have shown that aberrant oxygen homeostasis contributes to the renal pathology in pcy mice, but the underlying molecular mechanism remains largely unknown. Methods: pcy mice and a control strain, DBA/2N (DBA) mice, were used. Renal levels of 62 mRNAs involved in oxygen homeostasis were investigated by real-Time PCR, and the resulting data were used for extraction of pathological pathways. On the basis of the genes found to be upregulated and pathway analysis, further studies were performed using immunoblotting, immunohistochemistry, and pharmacological intervention. Results: In comparison with DBA mice, the levels of 18 mRNAs were altered by >2-fold in pcy mice. Pathway analysis extracted molecular pathways related to oxidative stress, inflammation, and cell adhesion. As the levels of mRNAs relevant to the NADPH oxidase 2 (NOX2) pathway were prominently (4 genes >5-fold) increased in pcy mice, we further analyzed the molecules related to this pathway. A time course study suggested that the pathway was gradually activated in pcy mice from at least 5 weeks of age. Immunohistochemistry study revealed that NOX2 protein was colocalized with a macrophage marker protein in the renal interstitium. Moreover, treatment of pcy mice with apocynin, an inhibitor of the NOX2 pathway, ameliorated the renal fibrosis. Conclusion: Our findings suggest that the activation of the NOX2 pathway, possibly mediated by macrophage infiltration, plays a pivotal role in progressive renal fibrosis in pcy mice.

    DOI: 10.1159/000520697

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  • Reduced urinary release of AQP1- and AQP2-bearing extracellular vesicles in patients with advanced chronic kidney disease Reviewed

    Oshikawa-Hori S., Yokota-Ikeda N., Sonoda H., Sasaki Y., Ikeda M.

    Physiological Reports   9 ( 17 )   e15005   2021.9

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Physiological Reports  

    Although several studies have shown that release of water channel proteins, aquaporin 1 (AQP1) and AQP2 in urinary extracellular vesicles (uEV-AQP1 and -AQP2), were altered in experimental kidney injury models, their release in human chronic kidney disease (CKD) has been largely unexplored. The aim of the present study was to clarify whether the release of uEV-AQP1 and -AQP2 is altered in patients with CKD. Urine samples were collected from 15 healthy volunteers (normal group) and 62 CKD patients who were categorized into six glomerular filtration rate (GFR) categories (G1, G2, G3a, G3b, G4, and G5) in between 2005 and 2016 at Miyazaki Prefectural Miyazaki Hospital, Japan. uEV-proteins were evaluated by immunoblot analysis. The release of AQP1 and AQP2 were significantly decreased in patients with both CKD G4 and G5, in comparison with the normal group. The area under the receiver operating characteristic (ROC) curve (AUC) values for AQP1 and AQP2 in patients with CKD G4 and G5 were 0.926 and 0.881, respectively. On the other hand, the AUC values in patients with CKD G1-G3 were 0.512 for AQP1 and 0.680 for AQP2. Multiple logistic regression analysis showed that AQP1 and AQP2 in combination were useful for detecting CKD G4 and G5, with a higher AUC value of 0.945. These results suggest that the release of uEV-AQP1 and -AQP2 was decreased in patients with CKD G4 and G5, and these proteins might be helpful to detect advanced CKD.

    DOI: 10.14814/phy2.15005

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  • Onion (Allium cepa l.)-derived nanoparticles inhibited lps-induced nitrate production, however, their intracellular incorporation by endocytosis was not involved in this effect on raw264 cells Reviewed

    Yamasaki M., Yamasaki Y., Furusho R., Kimura H., Kamei I., Sonoda H., Ikeda M., Oshima T., Ogawa K., Nishiyama K.

    Molecules   26 ( 9 )   2021.5

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Molecules  

    The aim of this study was to evaluate the involvement of nanoparticles prepared from Allium cepa L. as anti-inflammatory agents. In the present study, we identified nanoparticles from Allium cepa L. using the ultracentrifugation exosome purification method. The nanoparticles were referred to as 17,000× g and 200,000× g precipitates, and they contained quercetins, proteins, lipids, and small-sized RNA. The nanoparticles inhibited nitric oxide production from lipopolysaccharide (LPS)-stimulated RAW264 cells without cytotoxic properties. Cellular incorporation was confirmed by laser microscopic observation after PKH26 staining. The inhibition of caveolae-dependent endocy-tosis and macropinocytosis significantly prevented the incorporation of the nanoparticles but had no effect on the inhibition of nitric oxide in RAW264 cells. Collectively, the identified nanoparticles were capable of inhibiting the LPS response via extracellular mechanisms. Taken together, the way of consuming Allium cepa L. without collapsing the nanoparticles is expected to provide an efficient anti-inflammatory effect.

    DOI: 10.3390/molecules26092763

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  • Decreased Excretion of Urinary Exosomal Aquaporin-2 in a Puromycin Aminonucleoside-Induced Nephrotic Syndrome Model. Reviewed

    Abdeen A, Sonoda H, Kaito A, Oshikawa-Hori S, Fujimoto N, Ikeda M

    International journal of molecular sciences   21 ( 12 )   2020.6

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.3390/ijms21124288

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  • Release of urinary aquaporin-2-bearing extracellular vesicles is decreased in pregnant Japanese black cattle Reviewed

    Sinlapadeelerdkul T., Sonoda H., Uchida K., Kitahara G., Ikeda M.

    Journal of Veterinary Medical Science   81 ( 11 )   1609 - 1615   2019.11

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Journal of Veterinary Medical Science  

    © 2019 The Japanese Society of Veterinary Science. Aquaporin-2 (AQP2), a vasopressin-regulated water channel, plays an important role in renal water homeostasis. It has been reported that the level of AQP2 in human urine is altered during pregnancy. However, little is known about the level of urinary AQP2 in pregnant cattle. In this study, we examined the level of AQP2-bearing extracellular vesicles (uEV-AQP2), which account for most urinary AQP2, in both heifers and cows during the gestational and postpartum periods. The level of uEV-AQP2 was significantly decreased during gestation in comparison with the other cattle examined. Similarly, the levels of EV marker proteins in uEVs, including tumor susceptibility gene 101 (TSG101) protein and apoptosis-linked gene 2-interacting protein X (ALIX), were significantly decreased during gestation. There were significant correlations between the levels of uEV-AQP2 and uEV-TSG101, or uEV-ALIX. Immunohistochemistry data from pregnant and non-pregnant cattle supported the notion that the level of uEV-AQP2 was decreased during gestation. These data indicate that the level of uEV-AQP2 is decreased in pregnant cattle, possibly through a decrease in both the number of EVs released into the urine and renal AQP2 expression.

    DOI: 10.1292/jvms.19-0276

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Books 【 display / non-display

  • 大・中・小動物実験プロトコール

    池田正浩、園田紘子( Role: Joint author)

    宮日文化情報センター  2016.3 

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    Responsible for pages:80-82   Language:Japanese Book type:Scholarly book

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  • 尿中細胞外小胞(エクソソーム)と腎疾患 Invited

    園田紘子、池田正浩

    生体の科学   67 ( 5 )   406 - 407   2016.10

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    Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (scientific journal)   Publisher:医学書院  

  • Aquaporins in urinary extracellular vesicles (exosomes).

    Oshikawa S, Sonoda H, Ikeda M

    International journal of molecular sciences   2016.6

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    Language:English   Publishing type:Article, review, commentary, editorial, etc. (scientific journal)   Publisher:Molecular Diversity Preservation International Multidisciplinary Digital Publishing Institute  

    DOI: 10.3390/ijms17060957

  • 腎疾患におけるエクソソーム

    池田正浩, 園田紘子

    BIO Clinica   29 ( 6 )   30 - 33   2014.6

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    Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (scientific journal)   Publisher:北隆館  

  • Sepsis/MOFとAKI

    園田紘子, 髙橋早樹, 池田正浩

    ICUとCCU(集中治療医学)   34 ( 4 )   283 - 289   2010.4

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    Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (scientific journal)   Publisher:医学図書出版株式会社  

  • AKIと多臓器不全、敗血症

    園田紘子, 池田正浩, 池田直子

    臨床雑誌「内科」   102 ( 1 )   26 - 31   2008.7

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    Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (scientific journal)   Publisher:南江堂  

Presentations 【 display / non-display

  • A candidate molecule for non-invasive biomarkers in renal Nrf2 activation

    Haruka Takayama, Ayae Tanaka, Hiroko Sonoda, Masahiro Ikeda

    第95回日本薬理学会年会  2022.3.9 

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    Event date: 2022.3.7 - 2022.3.9

    Language:English   Presentation type:Poster presentation  

  • 急性腎不全モデルマウスにおけるポリマー沈殿法で単離した血中細胞外小胞mRNAの解析

    園田紘子、阪口優衣、礒見まり、宮原佑、池田正浩

    第74回日本薬理学会西南部会  2021.11.20 

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    Event date: 2021.11.20

    Language:Japanese   Presentation type:Poster presentation  

  • 伴侶動物の扁平上皮癌の悪性度とaquaporin 3発現との関連性についての検討

    園田紘子、谷口仁基、藤本成希、平井卓哉、松崎利行、伊東輝夫、 内田和幸、池田正浩

    第164回日本獣医学会学術集会  2021.9.7 

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    Event date: 2021.9.7 - 2021.9.13

    Language:Japanese   Presentation type:Oral presentation (general)  

  • イヌ乳腺腫瘍におけるアクアポリン1,3,5発現パターンの解析

    藤本成希、谷口仁基、園田紘子、平井卓哉、松崎利行、伊東輝夫、 内田和幸、池田正浩

    第164回日本獣医学会学術集会  2021.9.7 

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    Event date: 2021.9.7 - 2021.9.13

    Language:Japanese   Presentation type:Oral presentation (general)  

  • 血中細胞外小胞RNAに着目した腎肝クロストークモデルマウスにおけるバイオマーカー探索

    園田紘子、阪口優衣、礒見まり、宮原佑、池田正浩

    第12回トランスポーター研究会九州部会  2021.8.7 

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    Event date: 2021.8.7

    Language:Japanese   Presentation type:Poster presentation  

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Awards 【 display / non-display

  • 第66回日本薬理学会西南部会優秀発表賞

    2013.11   日本薬理学会西南部会  

    加藤綾華,田畑達彦,髙橋早樹,園田紘子,加藤丈司,北村和雄,池田正浩

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    Award type:Award from Japanese society, conference, symposium, etc.  Country:Japan

Grant-in-Aid for Scientific Research 【 display / non-display

  • 分子時計によるアクアポリン1発現調節機構の解明と腎障害への関与についての研究

    Grant number:21K05922  2021.04 - 2024.03

    科学研究費補助金  基盤研究(C)

    園田紘子

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    Authorship:Principal investigator 

  • 尿中exosome RNAsに着目した薬剤性腎障害診断マーカーの探索研究

    Grant number:15K18784   2015.04 - 2018.03

    科学研究費補助金  若手研究(B)

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    Authorship:Principal investigator 

    腎疾患は医療および獣医領域において罹患率および死亡率の高い疾患で、有用な早期診断マーカーがないことが問題となっている。本研究では非侵襲的に得ることのできる尿にexosomeと呼ばれる小胞が含まれることに着目した。このexosomeには由来細胞のタンパク質やmRNA、miRNA(RNAs)が含まれており、これらはその由来細胞の状態を反映したバイオマーカーとなる可能性が考えられる。本研究はタンパク質よりも解析が簡便なRNAsに着目し、薬剤性腎障害ラットモデルを用いて尿中exosome RNAsの腎疾患バイオマーカーとしての有用性を検討することを目的として行う。

  • 尿中エクソソームアクアポリン1およびアクアポリン2排泄の生理的調節機構の基礎研究

    Grant number:24780287  2012.04 - 2015.03

    科学研究費補助金  若手研究(B)

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    Authorship:Principal investigator 

    尿中には尿細管上皮細胞由来の小胞、エクソソームが存在しており、エクソソームにはその由来細胞のタンパク質やmRNAが含まれている。尿中エクソソームタンパク質やmRNAは、その由来する細胞の傷害における非侵襲的診断マーカーとして有用であると期待されている。申請者はこれまでに、尿中エクソソームに含まれる水チャネルアクアポリン1(AQP1)およびアクアポリン2(AQP2)タンパク質が急性腎障害早期診断マーカーとして有用である可能性を見出した。しかしながら、これらのタンパク質の生理的尿中排泄調節機構については明らかにされていない。本研究は、尿中エクソソームAQP1およびAQP2タンパク質、さらにはmRNAの診断マーカーとしての有用性を確立するためのバックグラウンドとしてこれらの生理的尿中排泄調節機構について明らかにすることを目的とする。