Associate Professor

Research Fields, Keywords

Veterinary Pharmacology

Field of expertise (Grants-in-aid for Scientific Research classification) 【 display / non-display

  • Veterinary medical science


Papers 【 display / non-display

  • Release of urinary aquaporin-2-bearing extracellular vesicles is decreased in pregnant Japanese black cattle

    Sinlapadeelerdkul T., Sonoda H., Uchida K., Kitahara G., Ikeda M.

    Journal of Veterinary Medical Science   81   1609 - 1615   2019.11  [Refereed]

    Joint Work

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    © 2019 The Japanese Society of Veterinary Science. Aquaporin-2 (AQP2), a vasopressin-regulated water channel, plays an important role in renal water homeostasis. It has been reported that the level of AQP2 in human urine is altered during pregnancy. However, little is known about the level of urinary AQP2 in pregnant cattle. In this study, we examined the level of AQP2-bearing extracellular vesicles (uEV-AQP2), which account for most urinary AQP2, in both heifers and cows during the gestational and postpartum periods. The level of uEV-AQP2 was significantly decreased during gestation in comparison with the other cattle examined. Similarly, the levels of EV marker proteins in uEVs, including tumor susceptibility gene 101 (TSG101) protein and apoptosis-linked gene 2-interacting protein X (ALIX), were significantly decreased during gestation. There were significant correlations between the levels of uEV-AQP2 and uEV-TSG101, or uEV-ALIX. Immunohistochemistry data from pregnant and non-pregnant cattle supported the notion that the level of uEV-AQP2 was decreased during gestation. These data indicate that the level of uEV-AQP2 is decreased in pregnant cattle, possibly through a decrease in both the number of EVs released into the urine and renal AQP2 expression.

    DOI PubMed CiNii

  • Urinary extracellular vesicular release of aquaporins in patients with renal transplantation

    Oshikawa-Hori S., Yokota-Ikeda N., Sonoda H., Ikeda M.

    BMC Nephrology   20   216   2019.06  [Refereed]

    Joint Work

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    © 2019 The Author(s). Background: Diuresis has been observed within a week following renal transplantation, suggesting that the procedure causes acute disturbance of renal water homeostasis. Aquaporin (AQP) 1 and AQP2, important proteins for renal water reabsorption, have been identified in urinary extracellular vesicles (uEV-AQP1 and -AQP2), and experimental studies have shown that the presence of uEV-AQP1 and -AQP2 may be an indicator of their levels of expression in the kidney. However, the release patterns of uEV-AQP1 and -AQP2 during the acute phase following renal transplantation are largely unknown. Methods: In this study, we examined the release of uEV-AQP1 and -AQP2 in recipients until 6 days (day 6) after renal transplantation. At Miyazaki prefectural Miyazaki Hospital, Japan, uEVs were obtained from 7 recipients, all of whom had received renal allografts from living donors. uEVs were isolated by differential centrifugation. Results: Immunoblotting analysis showed that the release of uEV-AQP2 was significantly decreased on day 1 in comparison with a control sample (from 3 healthy volunteers), accompanied by high urine output and low urine osmolality. Thereafter, the level increased gradually to the control level by day 6. The release pattern of uEV-AQP1 was similar to that of uEV-AQP2, but the levels did not reach statistical significance in comparison with the control level at any of the time points examined. Evaluation of the relationship between urinary osmolality and uEV-AQPs revealed a significant correlation for uEV-AQP2, but not for uEV-AQP1. Conclusion: These results indicate that acute diuresis after renal transplantation might be due to a decrease in the renal expression of AQP2, whose level can be estimated from the amount released in uEVs.

    DOI PubMed

  • A bell-shaped pattern of urinary aquaporin-2-bearing extracellular vesicle release in an experimental model of nephronophthisis

    Mikoda N., Sonoda H., Oshikawa S., Hoshino Y., Matsuzaki T., Ikeda M.

    Physiological Reports   7   e14092   2019.05  [Refereed]

    Joint Work

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    © 2019 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of The Physiological Society and the American Physiological Society. The DBA/2-FG pcy (pcy) mouse is a model of human nephronophthisis, a recessive cystic kidney disease. Renal expression of aquaporin-2 (AQP2), a water channel protein, has been shown to be altered in pcy mice. However, the relationship between the renal expression and its release in urinary extracellular vesicles (uEV-AQP2), which account for most urinary AQP2, in pcy mice has remained largely unknown. In this study, we examined age-related alterations of this relationship in pcy mice. In comparison with control mice, pcy mice after the age of 14 weeks showed defective urinary concentration ability with an increase in urinary volume. Interestingly, the release of uEV-AQP2 increased progressively up to the age of 16 weeks, but at 21 weeks the release did not significantly differ from that in control mice (i.e., a bell-shaped pattern was evident). Similar results were obtained for uEV marker proteins, including tumor susceptibility gene 101 (TSG101) protein and apoptosis-linked gene 2-interacting protein X (Alix). Immunoblot analysis revealed that renal AQP2 expression increased progressively from 11 weeks, and immunohistochemistry showed that this increase was possibly due to an increase in the number of AQP2-positive cells. Analysis of mRNAs for seven types of AQP expressed in the kidney supported this notion. These data suggest that the level of uEV-AQP2 does not simply mirror the renal expression of AQP2 and that the altered release of uEV-AQP2 in pcy mice depends on the numbers of both renal AQP2-positive cells and EVs released into the urine.

    DOI PubMed

  • miRNA profiling of urinary exosomes to assess the progression of acute kidney injury

    Sonoda H., Lee B., Park K., Nihalani D., Yoon J., Ikeda M., Kwon S.

    Scientific Reports   9   4692   2019.03  [Refereed]

    Joint Work

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    © 2019, The Author(s). Because exosomes have gained attention as a source of biomarkers, we investigated if miRNAs in exosomes (exo-miRs) can report the disease progression of organ injury. Using rat renal ischemia-reperfusion injury (IRI) as a model of acute kidney injury (AKI), we determined temporally-released exo-miRs in urine during IRI and found that these exo-miRs could reliably mirror the progression of AKI. From the longitudinal measurements of miRNA expression in kidney and urine, we found that release of exo- miRs was a regulated sorting process. In the injury state, miR-16, miR-24, and miR-200c were increased in the urine. Interestingly, expression of target mRNAs of these exo-miRs was significantly altered in renal medulla. Next, in the early recovery state, exo-miRs (miR-9a, miR-141, miR-200a, miR-200c, miR-429), which share Zeb1/2 as a common target mRNA, were upregulated together, indicating that they reflect TGF-β-associated renal fibrosis. Finally, release of exo-miRs (miR-125a, miR-351) was regulated by TGF-β1 and was able to differentiate the sham and IRI even after the injured kidneys were recovered. Altogether, these data indicate that exo-miRs released in renal IRI are associated with TGF-β signaling. Temporal release of exo-miRs which share targets might be a regulatory mechanism to control the progression of AKI.

    DOI PubMed

  • An Early Decrease in Release of Aquaporin-2 in Urinary Extracellular Vesicles After Cisplatin Treatment in Rats.

    Sonoda H, Oshikawa-Hori S, Ikeda M

    Cells   8   2019.02  [Refereed]

    Joint Work

    DOI PubMed

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Review Papers 【 display / non-display

  • Aquaporins in urinary extracellular vesicles (exosomes).

    Oshikawa S, Sonoda H, Ikeda M

    International journal of molecular sciences ( Molecular Diversity Preservation International Multidisciplinary Digital Publishing Institute )    2016.06

    Introduction and explanation (scientific journal)   Joint Work


Presentations 【 display / non-display

  • Decreased release of aquaporin-2 in urinary extracellular vesicles in rats subjected to allogenic kidney transplantation

    Hiroko Sonoda,Tomonori Nakanishi, Tomoyuki Kabayama, Sayaka Oshikawa, Masahiro

    Kidney Week 2018  2018.10  -  2018.10 

  • Release of urinary exosomal aquaporins in pregnant Japanese Black cattle

    Titaporn Sinlapadeelerkul、Hiroko Sonoda、 Go Kitahara、Masahiro Ikeda

    第13回トランスポーター研究会年会  2018.07  -  2018.07  喜多紗斗美

  • 腎疾患とエクソソーム

    園田紘子  [Invited]

    心血管代謝週間CVMW2017  2017.12  -  2017.12  佐田政隆、楽木宏美、三浦哲嗣

  • Endoplasmic reticulum stress induces aminoaciduria

    Moeko Koga, Ayaka Kato, Hiroko Sonoda, Sayaka Oshikawa, Masahiro Ikeda.

    Kidney Week 2017  2017.10  -  2017.11  Susan M. Wall

  • Extracellular Vesicular Release of Aquaporins in Rats after Kidney Transplantation

    Hiroko Sonoda, Tomonori Nakanishi, Tomoyuki Kabayama, Sayaka Oshikawa, Masahiro Ikeda

    Kidney Week 2016  2016.11  -  2016.11 

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