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Affiliation |
Faculty of Medicine School of Medicine Department of Medical Sciences, Chemistry |
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Professor |
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External Link |
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XU Yan
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Degree 【 display / non-display 】
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Doctor(Medicine) ( 2004.3 Tokyo Medical and Dental University )
Research Areas 【 display / non-display 】
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Nanotechnology/Materials / Chemical biology
Papers 【 display / non-display 】
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RNA structure promotes liquid-to-solid phase transition of short RNAs in neuronal dysfunction Reviewed
S. Y. Wang, Y. Xu
Commun. Biol. 2024.1
Authorship：Corresponding author Publishing type：Research paper (scientific journal)
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G-Quadruplexes in Human Telomere: Structures, Properties, and Applications Reviewed
Y. Xu, M. Komiyama
Molecules 2023.12
Authorship：Lead author, Corresponding author Publishing type：Research paper (scientific journal)
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A Unique G-Quadruplex Aptamer: A Novel Approach for Cancer Cell Recognition, Cell Membrane Visualization, and RSV Infection Detection Reviewed International coauthorship
C. D. Xiao, M. Q. Zhong, Y. Gao, Z. L. Yang, M. H. Jia, X. H. Hu, Y. Xu, X. C. Shen
Int. J. Mol. Sci. 2023.8
Publishing type：Research paper (scientific journal)
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Unusual topological RNA G-quadruplex formed by an RNA duplex: implications for the dimerization of SARS-CoV-2 RNA Reviewed International coauthorship
S. Y. Wang, Y. Song, Z. Y. He, H. Saneyoshi, R. Iwakiri, P. Y. Xu, C. Q. Zhao, X. G. Qu, Y. Xu
Chem. Commun. 2023.8
Authorship：Corresponding author Publishing type：Research paper (scientific journal)
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Unveiling the role of G-quadruplex structure in promoter region: Regulation of ABCA1 expression in macrophages possibly via NONO protein recruitment Reviewed International coauthorship International journal
Xiao C.D., Jia M.H., Zhong M.Q., Xu Y., Yu Z.T., He Z.Y., Lu X., Zhang Y., Zhou X., Fu L.Y., Shen X.C.
International Journal of Biological Macromolecules 245 ( 5 ) 119 - 128 2023.8
Language：English Publishing type：Research paper (scientific journal) Publisher：International Journal of Biological Macromolecules
ABCA1 has been found to be critical for cholesterol efflux in macrophages. Understanding the mechanism regulating ABCA1 expression is important for the prevention and treatment of atherosclerosis. In the present study, a G-quadruplex (G4) structure was identified in the ABCA1 promoter region. This G4 was shown to be essential for ABCA1 transcription. Stabilizing the G4 by ligands surprisingly upregulated ABCA1 expression in macrophages. Knocking out the G4 remarkably reduced ABCA1 expression, and abolished the increase of ABCA1 expression induced by the G4 ligand. By pull-down assays, the protein NONO was identified as an ABCA1 G4 binder. Overexpression or repression of NONO significantly induced upregulation and downregulation of ABCA1 expression, respectively. ChIP and EMSA experiments showed that the G4 ligand promoted the binding between the ABCA1 G4 and NONO, which led to more recruitment of NONO to the promoter region and enhanced ABCA1 transcription. Finally, the G4 ligand was shown to significantly reduce the accumulation of cholesterol in macrophages. This study showed a new insight into the regulation of gene expression by G4, and provided a new molecular mechanism regulating ABCA1 expression in macrophages. Furthermore, the study showed a possible novel application of the G4 ligand: preventing and treating atherosclerosis.
Books 【 display / non-display 】
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Oligonucleotide Containing 8-Trifluoromethyl-2′-Deoxyguanosine as a Z-DNA Probe (Z-DNA) International journal
Hong-Liang Bao & Yan Xu（ Role： Contributor）
Methods in Molecular Biology 2023.3 （ ISBN:978-4065207864 ）
Language：English Book type：Scholarly book
Z-DNA structure is a noncanonical left-handed alternative form of DNA, which has been suggested to be biologically important and is related to several genetic diseases and cancer. Therefore, investigation of Z-DNA structure associated with biological events is of great importance to understanding the functions of these molecules. Here, we described the development of a trifluoromethyl labeled deoxyguanosine derivative and employed it as a 19F NMR probe to study Z-form DNA structure in vitro and in living cells.
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In Cell 19F NMR for G-quadruplex (Handbook of Chemical Biology of Nucleic Acids) International journal
Yan Xu（ Role： Contributor）
Springer, Singapore 2023.1 （ ISBN:978-981-16-1313-5 ）
Language：English Book type：Scholarly book
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核酸科学ハンドブック(第II部 核酸科学の最前線 3. グアニン四重鎖構造)
徐岩（ Role： Contributor , 第II部 核酸科学の最前線 3. グアニン四重鎖構造）
講談社 2020.12 （ ISBN:978-4065207864 ）
Total pages：576 Language：Japanese Book type：Scholarly book
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T. Ishizuka, H. L. Bao, Y. Xu*（ Role： Joint author）
Springer 2019.8
Total pages：pp 407-433 Language：English Book type：Scholarly book
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Clipping of Telomere from Human Chromosomes Using a Chemistry-Based Artificial Restriction DNA Cutter（共著）
Takumi Ishizuka, Yan Xu, Makoto Komiyama（ Role： Joint author）
John Wiley & Sons, Inc. 2015.6
Language：English
MISC 【 display / non-display 】
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Human telomere DNA / RNA G-quadruplex and cancer therapy development Invited
2020.4
Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (scientific journal)
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Human Telomere RNA Structure and Function Reviewed
Y. Xu
Telomere and Telomerase 3 2016.11
Language：English Publishing type：Article, review, commentary, editorial, etc. (scientific journal)
DOI： 10.14800/tt.1455
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化学の目で、ヒトテロメアの謎を解く(共著)
徐岩
日本化学会生体関連部会ニュースレター 26 ( 1 ) 3 - 6 2011.6
Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (other) Publisher：日本化学会
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G-カルテット安定化分子でテロメラーゼを阻害する
Shinohara,K., Xu,Y., Sugiyama,H
化学 60 ( 7 ) 68 - 69 2005.7
Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (scientific journal) Publisher：化学同人
Presentations 【 display / non-display 】
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New discovery of Z-form DNA-RNA hybrid structure in vitro and in living cells and its function in DNA replication International conference
Shiyu Wang, Yan Xu
第50回国際核酸化学シンポジウム 2023.11.2
Event date： 2023.11.1 - 2023.11.3
Language：English Presentation type：Oral presentation (general)
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Unusual topological RNA G-quadruplex formed by an RNA duplex: implications for the dimerization of SARS-CoV-2 RNA International coauthorship International conference
Shiyu Wang, Yi Song, Zhiyong He, Hisao Saneyoshi , Rie Iwakiri , Pengyu Xu , Chuanqi Zhao , Xiaogang Qu, Yan Xu*
第50回国際核酸化学シンポジウム 2023.11.1
Event date： 2023.11.1 - 2023.11.3
Language：English Presentation type：Poster presentation
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Liquid to solid phase transition of short RNA promoted by RNA structure in neurodegenerative diseases. International conference
Shiyu Wang, Yan Xu
第49回国際核酸化学シンポジウム 2022.11.2
Event date： 2022.11.2 - 2022.11.4
Language：English Presentation type：Oral presentation (general)
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Combination of a near-infrared BODIPY derivative-based turn-on probe and a new norbornene-modified glucosamine derivative for in-cell and in vivo fluorescence imaging. International conference
Zhiyong He, Yan Xu
第49回国際核酸化学シンポジウム 2022.11.2
Event date： 2022.11.2 - 2022.11.4
Language：English Presentation type：Poster presentation
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Unusual topological RNA G-quadruplex formed by an RNA duplex: Implications for the dimerization of COVID-19 RNA. International conference
Yi Song, Shiyu Wang, Yan Xu
第49回国際核酸化学シンポジウム 2022.11.2
Event date： 2022.11.2 - 2022.11.4
Language：English Presentation type：Poster presentation
Awards 【 display / non-display 】
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宮崎大学ハイステップ研究者
2019.2 宮崎大学
徐岩
Award type：International academic award (Japan or overseas) Country：Japan
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光科学技術研究振興財団研究奨励金
2018.2 光科学技術研究振興財団
徐岩
Award type：Award from publisher, newspaper, foundation, etc. Country：Japan
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宮崎大学ハイステップ研究者
2016.12 宮崎大学
徐岩
Country：Japan
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武田科学振興財団研究奨励金
2013.8 武田科学振興財団
徐岩
Award type：Award from publisher, newspaper, foundation, etc. Country：Japan
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内藤記念科学振興財団研究奨励金
2011.12 内藤記念科学振興財団
徐岩
Award type：Award from publisher, newspaper, foundation, etc. Country：Japan
Grant-in-Aid for Scientific Research 【 display / non-display 】
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19F NMR分子技術の開発によるヒト細胞内DNA及びRNA高次構造の解明 International coauthorship
Grant number：21H02081 2021.04 - 2024.03
独立行政法人日本学術振興会 科学研究費補助金 基盤研究(B)
Authorship：Principal investigator
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クリック反応技術と軸配位子糖鎖連結ポリフィリン錯体を融合した革新的PDTの開発
Grant number：22K08806 2022.04 - 2025.03
独立行政法人日本学術振興会 科学研究費補助金 基盤研究(C)
七島 篤志、
Authorship：Coinvestigator(s)
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分子結合技術を用いた新たな造影剤による革新的がんMRI画像化技術の開発
Grant number：20K08000 2020.04 - 2023.03
独立行政法人日本学術振興会 科学研究費補助金 基盤研究(C)
Authorship：Coinvestigator(s)
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胆管癌の局所制御増強と光線過敏軽減による普及を目指した次世代光線力学的療法の開発
Grant number：19K09178 2019.04 - 2022.03
科学研究費補助金 基盤研究(C)
Authorship：Coinvestigator(s)
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テロメアRNA構造と反応性及びそれを活用したテロメアを標的とする新規癌治療の開発
Grant number：17H03091 2017.04 - 2020.03
科学研究費補助金 基盤研究(B)
Authorship：Principal investigator
Other research activities 【 display / non-display 】
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Advisory Board of Sci
2018.01
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Editorial Board of Molecules
2017.04
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"Faculty of 1000"のメンバー
2008.04
"Faculty of 1000" とは、生物学と医学研究の出版物において、最も重要だと思われる論文を認定し評価を与える国際学術組織です。
Available Technology 【 display / non-display 】
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生命分子機構を解明する革新的分子技術の開発
テロメアを標的とする革新的分子技術の開発
化学による革新的がん可視化診断技術の開発Home Page： 医学部機能制御学講座生命分子科学分野
Related fields where technical consultation is available：機能性核酸の創製、イメージング分子合成、細胞・動物分子イメージング、活性小分子の作製、短鎖ペプチドの合成
Message：・共同研究の希望テーマ：細胞・動物分子イメージング、人工核酸医薬、がん診断・治療