Affiliation |
Faculty of Agriculture Region of Animal and Plant Biosciences |
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Related SDGs |
TANAKA Hidenori
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Degree 【 display / non-display 】
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Doctor (Agriculture) ( 2006.3 Kagoshima University )
Research Interests 【 display / non-display 】
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Plant Genetic Resources and Breedings
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植物デジタル計測
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教学マネジメント
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Institutional Research
Research Areas 【 display / non-display 】
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Environmental Science/Agriculture Science / Science in plant genetics and breeding
Papers 【 display / non-display 】
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Arifin A.G., Shimamoto Y., Tanaka H., Hashiguchi M., Akashi R., Gondo T.
Legume Research 48 ( 1 ) 57 - 63 2025.1
Language:English Publishing type:Research paper (scientific journal) Publisher:Legume Research
Background: Identifying biomass-related genes to meet the increasing demand for food supply is important approach for agricultural and scientific efforts. In this study, we employed the Full-length cDNA Over-eXpressing gene hunting system (FOX hunting system) to identify biomass-related genes through ectopic expression of Arabidopsis thaliana full-length cDNA in bird’s-foot trefoil (Lotus corniculatus). Methods: A total of 66 FOX superroot lines (FSLs) from Lotus corniculatus cv. Viking were transferred in vermiculite after two weeks in half-strength Murashige and Skoog (MS) medium. These FSLs were then grown in a growth chamber for four weeks, during which their plant length and root length were measured. High biomass lines were selected from this group. FSL #83 and #121 were then grown for an additional four months in a greenhouse until they reached maturity, at which point they were evaluated for their agronomic traits. Result: Out of the 66 analyzed FSLs, 15 displayed superior growth characteristics in plant length and total root length. Arabidopsis cDNA, categorized into genes related to various functions such as membrane transport, photosystems, phytohormone synthesis, amino acid synthesis and unknown genes, was introduced into these lines. Two lines were grown to maturity in the greenhouse and their agronomic traits were analyzed in detail. FSL #83 expressed brassinosteroid biosynthetic pathway gene (CPD) led to significant differences in internode length, number of stems, number of flowers and above-ground dry matter weight compared to non-transgenic Super-growing roots (SR). On the other hand, FSL #121 expressed Asparaginyl-tRNA synthetase (SYNC1) gene exhibited increases in plant length and the number of stem nodes.
DOI: 10.18805/LRF-813
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Nakamura K., Gondo T., Chiba S., Akimoto S., Narushima J., Kondo K., Sugano Y., Tanaka H., Hashiguchi M., Shiwa Y., Akashi R.
Agriculture (Switzerland) 14 ( 12 ) 2024.12
Language:English Publishing type:Research paper (scientific journal) Publisher:Agriculture (Switzerland)
Gene expression in individual germinated seeds of four different soybean (Glycine max [L.] Merr.) cultivars, GL3494, OAC Kent, Williams 82, and Jackson, was examined using single seed high-throughput RNA sequencing (ssRNA-seq). The gene expression was similar between two individual seeds of the same cultivar, but different among individual seeds of the four cultivars. Notably, ssRNA-seq identified five genes that were not stably expressed in Williams 82, having either no detectable sequence reads or less than five sequence reads mapped to the exon regions of chromosomes in Williams 82. These findings were validated by reverse transcription polymerase chain reaction analysis. The study’s results demonstrate that gene expression in germinated seeds is unique to an individual seed from each soybean cultivar. This uniqueness may affect composition and content of nutrients in germinated soybeans used as food ingredients.
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Li F., Sayama T., Yokota Y., Hiraga S., Hashiguchi M., Tanaka H., Akashi R., Ishimoto M.
DNA Research 31 ( 2 ) 2024.4
Publishing type:Research paper (scientific journal) Publisher:DNA Research
Wild soybean (Glycine soja), the ancestor of the cultivated soybean (G. max), is a crucial resource for capturing the genetic diversity of soybean species. In this study, we used a set of 78 genome-wide microsatellite markers to analyse the genetic diversity and geographic differentiation patterns in a global collection of 2,050 G. soja accessions and a mini-core collection of G. max stored in two public seed banks. We observed a notable reduction in the genetic diversity of G. max compared with G. soja and identified a close phylogenetic relationship between G. max and a G. soja subpopulation located in central China. Furthermore, we revealed substantial genetic divergence between northern and southern subpopulations, accompanied by diminished genetic diversity in the northern subpopulations. Two clusters were discovered among the accessions from north-eastern China-one genetically close to those from South Korea and Southern Japan, and another close to those from Amur Oblast, Russia. Finally, 192 accessions were assigned to a mini-core collection of G. soja, retaining 73.8% of the alleles detected in the entire collection. This mini-core collection is accessible to those who need it, facilitating efficient evaluation and utilization of G. soja genetic resources in soybean breeding initiatives.
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Ng H.M., Gondo T., Tanaka H., Akashi R.
Plant Cell Reports 43 ( 2 ) 2024.2
Language:English Publishing type:Research paper (scientific journal) Publisher:Plant Cell Reports
Key Message: Genome editing by CRISPR/Cas9 can be applied to Z. matrella ‘Wakaba’, and knockout mutants of ZmNYC1 gene exhibited stay-green phenotype and reduced tillering. Abstract: Zoysia matrella is a widely used C4 warm-season turfgrass for landscaping, golf courses, and sports fields. Here, we used the CRISPR/Cas9 system to target the Non-Yellow Coloring1 (ZmNYC1) gene in the highly heterozygous allotetraploid Z. matrella ‘Wakaba’, aiming to generate a novel stay-green variety. Of 441 Agrobacterium-infected calli, 22 (5.0%) were transformed, and 14 of these (63.6%) showed targeted mutations through cleaved amplified polymorphic sequences analysis. Sequencing analysis revealed mutations mostly consisting of 1 or 2 bp indels, occurring 2 to 4 bp upstream of the PAM sequence. Regenerated plants exhibited five ZmNYC1 target locus genotypes, including homozygous mutants with a complete knockout of all four alleles in the T0 generation. Under dark treatment, ZmNYC1-mutated plants displayed suppressed chlorophyll b (Chl b) degradation, leading to higher chlorophyll content and Chl b, with a lower chlorophyll a/chlorophyll b ratio compared to the wild type (WT). However, the ZmNYC1 mutation also inhibited plant growth in homozygous mutant genotypes, exhibiting reduced tillering compared to WT. Additionally, during winter simulation, mutant with a complete knockout retained greenness longer than the WT. This is the first successful use of CRISPR/Cas9 genome editing in zoysiagrass. The mutants of the ZmNYC1 gene would serve as valuable breeding material for developing improved zoysiagrass varieties that can maintain their green color for longer periods, even during winter dormancy.
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Bungsrisawat P., Tumwasorn S., Loongyai W., Nakthong S., Nitthaisong P., Tanaka H., Akashi R., Sopannarath P.
Animal Science Journal 95 ( 1 ) 2024.1
Publishing type:Research paper (scientific journal) Publisher:Animal Science Journal
Betong chicken (KU line) is a slow-growing Thai native chicken used for meat production. The objectives of this study were to identify polymorphisms of the calpain1 (CAPN1) and calpain3 (CAPN3) genes and to investigate their effects on growth, carcass, and meat quality traits in Betong chickens (KU line). A sample of 252 Betong chickens (KU line) was screened for CAPN1 and CAPN3 polymorphisms. The polymorphisms of CAPN1 were detected using gel electrophoresis and DNA sequencing, whereas the polymorphisms of CAPN3 were identified using restriction fragment length polymorphism. Polymorphisms were detected in both CAPN1 (AA, AB, and BB genotypes) and CAPN3 (CC, CT, and TT genotypes). The frequency of the B allele was higher than for the A allele (0.78 and 0.22, respectively) in CAPN1, while the C allelic frequency was higher than for the T allele (0.54 and 0.46, respectively) in CAPN3. The CAPN1 genotype and the combination of the CAPN1 and CAPN3 genotypes could be used as genetic markers for meat lightness. The CAPN3 could be useful for increasing body weight, live weight, and breast meat weight in Betong chickens (KU line).
DOI: 10.1111/asj.13986
Books 【 display / non-display 】
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形質転換プロトコール《植物編》
田部井 豊 編( Role: Joint author , セイヨウミヤコグサ)
化学同人 2012.9
Language:Japanese Book type:Scholarly book
MISC 【 display / non-display 】
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Actual Status of Research Equipment Usage Inside and Outside the University and Analysis of Research Papers Published by on-Campus Use of Research Equipment at the University of Miyazaki
SAKAI Kentaro, TANAKA Hidenori
The Journal of Science Policy and Research Management 39 ( 1 ) 26 - 34 2024.5
Language:Japanese Publishing type:Rapid communication, short report, research note, etc. (scientific journal) Publisher:Japan Society for Research Policy and Innovation Management
In order to improve research capabilities of Japan, it is necessary to strategically maintain, operate, and share research facilities and equipment based on university management strategies. In this situation, it is important to establish institutional research (IR) of research infrastructure that quantitatively analyzes and evaluates how the use of research equipment contributes to the research capabilities of universities, but this is still in the development stage. Therefore, it is necessary to improve its functions and establish a methodology. In this paper, we report the usage of research equipment inside and outside the university obtained from the equipment management system developed at the University of Miyazaki, and analyze the research fields, analysis contents, and regional characteristics of analysis request commissioned from outside the university. Moreover, we will analyze the number and quality of papers published through the use of research facilities, and describe an example of research infrastructure IR at our university, which is a local/regional university.
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田中秀典
大学時報 ( 405 ) 78 - 83 2022.7
Authorship:Lead author Language:Japanese Publishing type:Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media)
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NBRPミヤコグサ・ダイズにおけるバイオリソース整備 Invited
橋口 正嗣,田中 秀典,橋口 拓勇,明石 良
農業および園芸 96 ( 2 ) 153 - 162 2021.2
Language:Japanese Publishing type:Article, review, commentary, editorial, etc. (scientific journal)
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マメ科植物研究のためのミヤコグサバイオリソース Invited Reviewed
橋口正嗣,佐藤修正,橋口拓勇,田中秀典,明石 良
植物科学の最前線(BSJ-Review) 10 179 - 189 2019.7
Language:Japanese Publishing type:Article, review, commentary, editorial, etc. (scientific journal) Publisher:日本植物学会
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ダイズ成長過程における形質・環境計測値の相互関係のネットワーク解析
七夕高也, 磯部祥子, 林篤司, 田中秀典, 橋口正嗣, 明石良, 長谷川舞衣, 菊地正隆, 中谷明弘, 佐藤修正, 七夕小百合
育種学研究 20 97 2018.3
Language:Japanese Publishing type:Research paper, summary (national, other academic conference)
Presentations 【 display / non-display 】
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Development of a small-scale imaging system for plant 3D model reconstruction using zoysiagrass International conference
Pongpiyapaiboon Sorawich, Hidenori Tanaka
11th International Conference on Agriculture 2024 (AGRICO 2024)
Event date: 2024.9.23 - 2024.9.24
Language:English Presentation type:Oral presentation (general)
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Effects of dust-coated water-retaining agent on the rooting of soybean (Glycine max) seeds under dry conditions International conference
Rikako Umino, Kanade Kawaguchi, Hidenori Tanaka, Genki Ishigaki
11th International Conference on Agriculture 2024 (AGRICO 2024)
Event date: 2024.9.23 - 2024.9.24
Language:English Presentation type:Poster presentation
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イネのデジタル3Dモデルによる計測法の開発
Sorawich Pongpiyapaiboon, 田中 秀典, 平野 翔也, 貴島 祐治, 明石 良
日本育種学会第144回講演会 2023.9.17
Event date: 2023.9.16 - 2023.9.18
Presentation type:Oral presentation (general)
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デジタル3Dモデルを用いた同一イネ品種の2倍体と4倍体成長パターンの比較
平野 翔也, Sorawich Pongpiyapaiboon, 笹川 静香, 高牟禮 逸朗, 田中 秀典, 明石 良, 貴島 祐治
日本育種学会第144回講演会 2023.9.17
Event date: 2023.9.16 - 2023.9.18
Presentation type:Oral presentation (general)
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シバ属における3Dモデルから抽出した葉色情報とNDVIの相関
Pongpiyapaiboon Sorawich, 田中 秀典, 橋口 拓勇, 橋口 正嗣, 明石 良
日本育種学会第142回講演会 2022.9.23
Event date: 2022.9.23 - 2022.9.24
Language:English Presentation type:Oral presentation (general)
Grant-in-Aid for Scientific Research 【 display / non-display 】
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学生の学業継続および退学予測モデルの構築に関する実証的研究
Grant number:23K22296 2024.04 - 2025.03
独立行政法人日本学術振興会 科学研究費基金 基盤研究(B)
Authorship:Coinvestigator(s)
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ゲノム編集食品が遺伝子組み換え食品と同じ轍を踏まないために
Grant number:23K05404 2023.04 - 2027.03
独立行政法人日本学術振興会 科学研究費補助金 基盤研究(C)
Authorship:Coinvestigator(s)
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学生の学業継続および退学予測モデルの構築に関する実証的研究
Grant number:22H01025 2022.04 - 2025.03
独立行政法人日本学術振興会 科学研究費補助金 基盤研究(B)
Authorship:Coinvestigator(s)
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大学の数量的な「共通知」から分析マインドを涵養する人材育成プラットフォームの開発
Grant number:18K02706 2018.04 - 2022.03
科学研究費補助金 基盤研究(C)
Authorship:Coinvestigator(s)
Available Technology 【 display / non-display 】
Social Activities 【 display / non-display 】
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山形大学・履修証明プログラム(IR担当者向け実践プログラム)担当講師
Role(s): Lecturer
山形大学 2024.5 - 2024.10
Audience: Teachers, Researchesrs, General, Governmental agency
Type:Certification seminar
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山形大学・履修証明プログラム(IR担当者向け実践プログラム)担当講師
Role(s): Lecturer
山形大学 2023.5 - 2023.10
Audience: Teachers, Researchesrs, General, Governmental agency
Type:Certification seminar
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山形大学・履修証明プログラム(IR担当者向け実践プログラム)担当講師
Role(s): Lecturer
山形大学 2022.5 - 2022.9
Audience: Teachers, Researchesrs, General, Governmental agency
Type:Certification seminar
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山形大学・履修証明プログラム(IR担当者向け実践プログラム)担当講師
Role(s): Lecturer
山形大学 2021.5 - 2021.9
Audience: Teachers, Researchesrs, General, Governmental agency
Type:Certification seminar
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山形大学・履修証明プログラム(IR担当者向け実践プログラム)ゲスト講師
Role(s): Lecturer
山形大学 2020.11.28
Audience: Teachers, Researchesrs, General, Governmental agency
Type:Certification seminar