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Faculty of Agriculture Department of Marine Biological and Environmental Science |
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Professor |
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TAOKA Yosuke
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Degree 【 display / non-display 】
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Doctor (Fishery) ( 2007.3 Kagoshima University )
Research Areas 【 display / non-display 】
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Life Science / Applied microbiology
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Life Science / Aquatic bioproduction science
Papers 【 display / non-display 】
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Yasaka Y., Binh P.T., Hayami Y., Tanaka R., Uchida K., Taoka Y.
Fisheries Science 89 ( 1 ) 41 - 51 2022.12
Authorship：Corresponding author Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Fisheries Science
We focused on developing an epidemic prevention method for circulating masu salmon aquaculture using the probiotic Lactococcus lactis strain K-C2. This study aimed to evaluate the antibacterial activities of strain K-C2 against pathogens isolated from dead Yamame and masu salmon. First, we identified pathogenic bacteria that were isolated from dead masu salmon based on phylogenetic analysis of the 16S rRNA gene sequence and biological and biochemical characterization. The antibacterial activity of strain K-C2 against seven pathogenic strains isolated from dead masu salmon in this study and two strains of Aeromonas salmonicida previously isolated from dead Yamame was tested using a double agar plate method. The results of a BLAST search using 16S rRNA partial sequence data (1200–1300 bp) revealed that six of the former strains and one of the latter strains showed high similarity to Vibrio anguillarum and Tenacibaculum maritimum, respectively. Strain K-C2 showed antibacterial activity against all pathogenic bacteria. In this study, pathogenic bacteria were newly isolated from dead seawater-acclimated masu salmon, and strain K-C2 was found to have antibacterial effects against these pathogens.
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Effect of the probiotic Lactococcus lactis subsp. lactis strain K-C2 on the release of amino acids from aquafeeds, and on the intestinal microflora of amberhack, Seriola dumerili and common carp, Cyprinus carpio following administration as a feed additive Invited Reviewed International coauthorship
Yousuke Taoka, Yuki Hayami, Phan Trong Binh, Yurie Yasaka
2022.12
Authorship：Lead author, Corresponding author Language：English Publishing type：Research paper (scientific journal)
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Yanohara T., Taoka Y., Yamamoto M.
Fermentation 8 ( 5 ) 2022.5
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Fermentation
The internal organs of white sturgeon in Miyazaki Prefecture are discarded during processing. Therefore, we tried to produce fish sauce using a short-term manufacturing method. The minced internal organs were autolyzed by endogenous proteases at 50 °C. During autolysis, the protein contents of the supernatant and precipitate after centrifugation were analyzed by the Kjeldahl method, and the protein size was monitored by SDS-PAGE. This analysis showed that the extraction rate was about 60% after treatment at 50 °C for 24 h. The major bands at 200 kDa, 43 kDa, and 40 kDa detected before the start of the treatment gradually disappeared over time. Fifteen components were detected as the main volatile components. These components increased sharply and then decreased during incubation at 50 °C for 24 h. The fish sauce produced had a good aroma after incubation at 50 °C for 72 h.
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Evaluation of Rate of Adhesion of Lactobacillus namurensis Strain GYP-74 to Porous Fine Ceramics Invited Reviewed
Yousuke Taoka, Kentaro Sakai, Hiroyuki Kinoshita, Hanaka Fukuyama, Taichi Kobayashi, Atsushi Hirano, Noriaki Kuroki, Akihiko Kimura
Processes 9 ( 4 ) 658 2021.4
Language：English Publishing type：Research paper (scientific journal)
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Evaluation of rate of adhesion of Lactobacillus namurensis strain GYP-74 to porous fine ceramics
Taoka Y., Sakai K., Kinoshita H., Fukuyama H., Kobayashi T., Hirano A., Kuroki N., Kuroki M., Kimura A.
Processes 9 ( 4 ) 2021.4
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Processes
This study aimed to evaluate the ratio of adherence of lactic acid bacteria (LAB) to porous fine ceramics in order to develop a novel LAB-rich pickle container for the production of functional fermented vegetables. Some LAB were isolated from the salted rice bran used for pickling (Nukadoko in Japanese). These isolates were classified in Lactobacillus namurensis by phylogenetic analysis. Some pottery-shard (PS) samples were prepared by varying the mixing rate of polyacetal (POM) resin to clay (0-30% (v/v)) and the burning temperature (1000 °C or 1100 °C). A test of the adherence of strain GYP-74 to the PSs was performed. The results showed that the adherence rate was significantly higher in the PSs burned at 1100 °C as compared with those burned at 1000 °C. A pore distribution analysis showed that pore sizes of less than a few µm and pore sizes in the range of a few µm to a few hundred µm were mainly distributed in the PSs without and with POM, respectively. X-ray diffraction analysis showed that both PSs with and without POM contained quartz and hematite. The PSs burned at 1000 °C and 1100 °C specifically contained microcline and mullite, respectively. This study revealed the basal information regarding what makes PSs adequate for LAB adhesion.
DOI： 10.3390/pr9040658
Books 【 display / non-display 】
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水産増養殖におけるプロバイオティクスの可能性と物質代謝から見た作用機序の解明
田岡洋介・Nguyen Thi Hue Linh（ Role： Joint author）
北隆館 2020.10
Language：Japanese Book type：Scholarly book
MISC 【 display / non-display 】
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水産増養殖におけるプロバイオティクスの可能性と物質代謝から見た作用機序の解明 Invited International coauthorship
田岡洋介・Nguyen Thi Hue Linh
アグリバイオ 2021.5
Authorship：Lead author Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media)
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Taoka Y., Nagano N., Okita Y., Izumida H., Sugimoto S., Hayashi M.
Journal of Oleo Science 60 ( 5 ) 217 - 220 2011
Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (bulletin of university, research institution) Publisher：Journal of Oleo Science
To examine the effect of cold shock treatment on the fatty acid composition of Aurantiochytrium limacinum strain mh0186, a marine thraustochytrid, we cultivated this strain at 28°C for 72 h with shaking and stored the obtained biomass at 10°C for 72 h. A growth experiment was carried out for comparison, wherein strain mh0186 was grown at 10 and 15°C for 72 h with shaking, and it was found that the unsaturation of fatty acids was accelerated relative to that at 28°C. In the cold shock experiment, the total lipid content signifi cantly increased during storage at 10°C for 72 h. Overall, the percentage of unsaturated fatty acids such as docosahexaenoic acid was almost stable while that of n-6 docosapentaenoic acid decreased slightly, but signifi cantly, relative to that in the growth experiment. © 2011 by Japan Oil Chemists' Society.
DOI： 10.5650/jos.60.217
Presentations 【 display / non-display 】
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チョウザメ魚醤の速醸化の検討
矢野原泰士・田岡洋介・梅津和歩・山本瑞貴＊ 田島良亮・黒田明大・細田蓮也
日本水産学会春季大会 (オンライン開催（大会本部：東京海洋大学内）) 日本水産学会
Event date： 2021.3.26 - 2021.3.30
Language：English Presentation type：Oral presentation (general)
Venue：オンライン開催（大会本部：東京海洋大学内）
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Complete replacement of fishmeal in aquafeeds for carp, Cyprinus carpio with lipid-extracted residue derived from a thraustochytrid Aurantiochytrium sp. mem0039 Invited International conference
Noriyuki Tai, Horii Kenya, Yousuke Taoka
International Conference on Aquaculture and Fisheries in Bangkok (Bangkok, Thailand)
Event date： 2019.11.11 - 2019.11.12
Language：English Presentation type：Oral presentation (invited, special)
Venue：Bangkok, Thailand
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プロバイオティクスを用いた魚病予防法の開発
田岡洋介
動物用ワクチン-バイオ医薬品研究会 シンポジウム
Event date： 2019.9.12
Language：English Presentation type：Oral presentation (general)
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Evaluation of light-emitting diode irradiation on the inhibitory effect against fish pathogens International conference
Phan Trong Binh, Satoshi Hori, Yousuke Taoka
Marine Biotechnology Conference 2019
Event date： 2019.9.9 - 2019.9.13
Language：English Presentation type：Poster presentation
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Enrichment of Lactcoccus lactis strain K-C2 on Artemia and inhibition against Vibrio alginolyticus, V. campbellii and V. harveyi by strain K-C2 International conference
Yuki Hayami, Nguyen Thi Hue Linh, Yousuke Taoka
Marine Biotechnology Conference 2019 (Shizuoka, Japan)
Event date： 2019.9.9 - 2019.9.13
Language：English Presentation type：Poster presentation
Venue：Shizuoka, Japan
Awards 【 display / non-display 】
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平成23年度日本水産学会論文賞
2012.3 公益社団法人日本水産学会
田岡洋介、前田広人、Jae-Yoon JO、Min-Jee JEON、Sungchul C. BAI、Won-Jae LEE、弓削寿哉、越塩俊介
Award type：International academic award (Japan or overseas) Country：Japan
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平成18年度日本防菌防黴学会論文賞
2006.5 鹿児島大学
塩崎靖志、前田広人、田岡洋介、越塩俊介、吉川毅、坂田泰造、C. Saclauso
Award type：Award from Japanese society, conference, symposium, etc. Country：Japan
Other research activities 【 display / non-display 】
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宮崎県西都産養殖鯉を用いた機能性魚醤油の開発
2021.04 - 2022.03
全国3位を誇る西都産養殖鯉の高度利用化を図るため、本養殖鯉を用いた機能性魚醤油並びにエキスの開発に関する研究を実施した。
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平成30年度拡充（ソフト）事業"細島いわがき"成分検査事業
2018.04 - 2019.03
日向市との共同研究「平成30年度拡充（ソフト）事業"細島いわがき"成分検査事業」を実施、1年間を通して養殖牡蠣の成分評価を行なった。
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TECH PLANTER 2018 宮崎大会
2017.12
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宮崎県日向市沿岸の水質調査とブランド化に関わる研究
2015.04
COC地域貢献研究事業のもと、日向市と協同して特産水産物であるハマグリのブランド化（DHA強化ハマグリ作出）に関する研究を行った。またハマグリ資源管理のため、ハマグリ生息域である沿岸部の海水質調査を行った。
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宮崎県高鍋町沿岸海水質調査
2015.04
宮崎コンソーシアム公募型卒論テーマとして、高鍋町との協同の元、高鍋沿岸域の海水質調査を行った。地場水産有用種であるカキの生息環境としての高鍋沿岸域を評価するとともに、資源保持のための施策を行政と連動して検討する。なお本研究テーマを担当した学生が同年度のコンソーシアム報告会にて口頭発表し、最優秀賞を受賞した。
Available Technology 【 display / non-display 】
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乳酸菌を用いた機能性成分強化発酵食品の開発
宮崎県産水産物を用いた魚醤の開発Home Page： 研究室ホームページ
Related fields where technical consultation is available：・発酵微生物の単離・同定や最適培養条件の探索
・水産物・農作物の発酵試験、成分分析（アミノ酸分析など）Message：・共同研究の希望テーマ：微生物を用いた新規発酵食品の開発
・「素材の発酵食品化」というニーズがあれば、ぜひ教えてください。
・食品加工残渣（未利用資源）を利用した食品の開発ニーズがあれば、ぜひご連絡ください。