Presentations -
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Research on Probiotics in Aquaculture Invited International conference
Yousuke Taoka
Meetting 20250111@MFQAA 2025.1.10
Event date： 2025.1.10
Language：English Presentation type：Oral presentation (general)
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Lactococcus lactis subsp. lactis K-C2株による 養魚飼料由来代謝物のメタボローム解析 International coauthorship
早見 祐紀・並木よしの・Phan Trong Binh・八坂友里恵・ 田岡洋介
日本水産学会九州支部大会 2023.12.9
Event date： 2023.12.8 - 2023.12.9
Language：Japanese Presentation type：Oral presentation (general)
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Effect of oral administration of Aurantiochytrium sp. strain mem0039 cells on immune responses of common carp, Cyprinus carpio Invited International conference
Yousuke Taoka, Ms. Shizuki Okamoto, Mungkornpan Santiwijai
The 11th Annual World Congress of Ocean 2023.11.17
Event date： 2023.11.15 - 2023.11.17
Language：English Presentation type：Oral presentation (general)
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チョウザメ魚醤の速醸化の検討
矢野原泰士・田岡洋介・梅津和歩・山本瑞貴＊ 田島良亮・黒田明大・細田蓮也
日本水産学会春季大会 (オンライン開催（大会本部：東京海洋大学内）) 日本水産学会
Event date： 2021.3.26 - 2021.3.30
Language：English Presentation type：Oral presentation (general)
Venue：オンライン開催（大会本部：東京海洋大学内）
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Complete replacement of fishmeal in aquafeeds for carp, Cyprinus carpio with lipid-extracted residue derived from a thraustochytrid Aurantiochytrium sp. mem0039 Invited International conference
Noriyuki Tai, Horii Kenya, Yousuke Taoka
International Conference on Aquaculture and Fisheries in Bangkok (Bangkok, Thailand)
Event date： 2019.11.11 - 2019.11.12
Language：English Presentation type：Oral presentation (invited, special)
Venue：Bangkok, Thailand
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プロバイオティクスを用いた魚病予防法の開発
田岡洋介
動物用ワクチン-バイオ医薬品研究会 シンポジウム
Event date： 2019.9.12
Language：English Presentation type：Oral presentation (general)
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Evaluation of light-emitting diode irradiation on the inhibitory effect against fish pathogens International conference
Phan Trong Binh, Satoshi Hori, Yousuke Taoka
Marine Biotechnology Conference 2019
Event date： 2019.9.9 - 2019.9.13
Language：English Presentation type：Poster presentation
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Enrichment of Lactcoccus lactis strain K-C2 on Artemia and inhibition against Vibrio alginolyticus, V. campbellii and V. harveyi by strain K-C2 International conference
Yuki Hayami, Nguyen Thi Hue Linh, Yousuke Taoka
Marine Biotechnology Conference 2019 (Shizuoka, Japan)
Event date： 2019.9.9 - 2019.9.13
Language：English Presentation type：Poster presentation
Venue：Shizuoka, Japan
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Identification and characterization of pathogenic bacteria isolated from dead masu salmon, Oncorhynchus masou masou and antibacterial activity of probiotic Lactococcus lactis strain K-C2 against isolated pathogens International conference
Yurie Yasaka, Phan Trong Binh, Ryusuke Tanaka, Katsuhisa Uchida, Yousuke Taoka
Marine Biotechnology Conference 2019
Event date： 2019.9.9 - 2019.9.13
Language：English Presentation type：Poster presentation
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Production of Anserine-Rich Fish Sauce from Giant Masu Salmon, Oncorhynchus masou masou for Prevention of Locomotive Syndrome International conference
Yousuke Taoka
SCON FOOD SCIENCE 2019 (Singapore)
Event date： 2019.6.27 - 2019.6.28
Language：English Presentation type：Oral presentation (general)
Venue：Singapore
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宮崎県日向市沿岸における養殖牡蠣の成長、グリコーゲン、ミネラル 及びアミノ酸組成の季節的変動
小原有紀,濵㟢菜々子,松本明子,境健太郎, 黒木信介,田岡洋介
平成31年度日本水産学会春季大会 (東京海洋大学) 日本水産学会
Event date： 2019.3.27 - 2019.3.31
Language：English Presentation type：Poster presentation
Venue：東京海洋大学
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Comprehensive Production and Application Technology of both Lipid and Protein Derived from Eukaryotic microbes, Thraustochytrids Invited International conference
Yousuke Taoka, Noriyuki Tai
[IMT-GT 2018 (Penang, Malaysia) IMT-GT
Event date： 2018.12.11 - 2018.12.12
Language：English Presentation type：Oral presentation (invited, special)
Venue：Penang, Malaysia
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Development of Fishmeal-Free Aquafeeds with Thraustochytrids-Protein Invited International conference
Noriyuki Tai, Kenya Horii and Yousuke Taoka
BIT’s 8th Annual World Congress of Aquaculture and Fisheries-2018 (Weihai, China) BIT'S
Event date： 2018.10.29 - 2018.10.31
Language：English Presentation type：Oral presentation (general)
Venue：Weihai, China
油糧生産微生物ラビリンチュラの培養細胞からオイル抽出した残渣をタンパク源として、養魚用の配合飼料を作成した。配合飼料で羽生されている魚粉を用いた試料も同様に作成し、コイに対する飼育試験を実施した。その結果、ラビリンチュラ抽出残渣は魚粉で作成した飼料と同等の成長を示した。
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Production of Anserine-Rich Fish Sauce from Giant Masu Salmon, Oncorhynchus masou masou International conference
Yousuke Taoka
BIT’s 6th Annual World Congress of Aquaculture and Fisheries-2017 (Shenzhen, China)
Event date： 2017.11.3 - 2017.11.5
Language：English Presentation type：Oral presentation (general)
Venue：Shenzhen, China
Recently, Dr. Katsuhisa Uchida who belong to University of Miyazaki, Japan, succeeded in establishing a production technique for giant masu salmon by transferring landlocked yamame to the sea for rearing during the winter season. The production rate and yield of the fish-mass significantly increased by culturing in seawater. The purpose of this study was to produce a fish sauce from newly developed fishery source, giant mass salmon by rapid fermentation under no-salt conditions. The whole body of giant masu salmon except for eggs was minced well, and the activity of protease contained in the mixtures was analyzed at 55oC and 60oC according to the adequate temperature indicated by our previous study. The protease activity was comparable to our previous study, and the activity at 55 and 60oC was 33 and 49 Unit/ g-protein, respectively. Giant masu salmon mixtures were incubated to induce autolysis at 55 or 60oC for 72 hrs. During incubation, to evaluate the degree of autolysis, the amino acid composition was analyzed with liquid chromatograph-mass spectrometry (LC-MS). The distribution of protein molecular size was analyzed by SDS-PAGE. The content of total amino acids was increased as time passed, and the low molecular weight bands appeared by incubation. Giant masu salmon mixtures were incubated at 60oC for 24 h, and the centrifugal supernatant was heated in boiling water for 20 min. The supernatant of the heated solution was used as giant masu salmon fish sauce (GMSFS). The ratio of anserine which well known as a functional amino acid, in GMSFS was significantly high (≒48% to total amino acid). Thus, the anserine-rich fish sauce could be produced quickly from giant masu salmon. At present, to add more functionality to GMSFA and modify the taste, we are carrying out the fermentation trial of GMSFS with lactic acid bacteria after autolysis.
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Enrichment of probiotic Lactococcus lactis strain K-C2 in Artemia sp. International conference
Yuki Hayami, Nguyen Thi Hue Linh, Yousuke Taoka
International Symposium Fisheries Science for Future Generation (東京海洋大学) 日本水産学会
Event date： 2017.9.22 - 2017.9.24
Language：English Presentation type：Poster presentation
Venue：東京海洋大学
By previous our study, Lactococcus lactis strain K-C2 showed antagonistic activities against various pathogens in aquaculture such as Edwardsiella tarda, Lactococcus garviae and Streptococcus iniae. Therefore, this strain is considered as novel probiotics to prevent disease in aquaculture. In this study, we tried to enrich L. lactis strain K-C2 in the feed organisms, Artemia sp. Commercial eggs of Artemia (Pacific Trading Co. Ltd., Fukuoka, Japan) were hatched in an artificial seawater under the vigorous aeration and light irradiation. The Artemia after 4 days of hatching was introduced to 80 ml of artificial seawater in a flask (26 individuals/ml). Strain K-C2 was added to the seawater containing Artemia at 3.2×108 cfu/ml. As a control group, Artemia was cultured without addition of strain K-C2. After exposure to the cell suspensions of strain K-C2, Artemia was harvested and washed by sterile artificial seawater and homogenized. The homogenates was serially diluted, and each dilutions were inoculated on a GYP agar plate medium containing calcium carbonate. The plates were incubated at 28oC for 4 days and viable colonies were counted to calculate colony forming unit (CFU). The colonies which degraded the calcium carbonate were randomly selected and colony PCR was carried out using Lactococcus specific primer set (Forward primer, LacF 5’ GTACTTGTACCGACTGGAT 3’ and reverse primer, LacreR 5’ GGGATCATCTTTGAGTGAT 3’) to detect the strain K-C2. The selected colonies were estimated as L. lactis by PCR with specific primers. The number of L. lactis in Artemia was calculated to 4.2×103 CFU/ 100 individuals. This study indicated that probiotic L. lactis strain K-C2 could be enriched in Artemia by exposure in seawater.
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Rearing Trial of Eel, Anguilla japonica in a Closed Recirculating System Using Hot Spring Water Welled up in Saito City, Miyazaki, Japan
Seika Okane, Kaho Umezu, Mitsuki Horoiwa, Yukie Kamata, Yuki Hayami1, Setsuko Nagai1, Noriko Nagasaka1, Kenya Horii, Nguyen Thi Hue Linh, Ryusuke Tanaka, Katsuhisa Uchida Yousuke Taoka
International Symposium Fisheries Science for Future Generation (東京海洋大学) 日本水産学会
Event date： 2017.9.22 - 2017.9.24
Language：English Presentation type：Poster presentation
Venue：東京海洋大学
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Development of Probiotics in Aquaculture Invited International conference
Yousuke Taoka
Information regarding bilateral conference between University of Miyazaki and NPUST (NPUST) NPUST
Event date： 2017.5.3
Language：English Presentation type：Oral presentation (general)
Venue：NPUST
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Relationship of Amino Acid Composition, Fatty Acid Composition and Phylogenetic Classification in Marine Eukaryotes, Thraustochytrids International conference
Kenya Horii, Masahiro Hayashi, Yousuke Taoka
The 10th IMT-GT UNINET Conference 2016
Event date： 2016.11.30 - 2016.12.2
Language：English Presentation type：Oral presentation (general)
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Possibility of Marine Eukaryotes, Thraustochytrids as Protein Sources for Non-Fishmeal Aquafeed in Aquaculture Invited International conference
Yousuke Taoka, Kenya Horii, Masahiro Hayashi
BIT’s 5th Annual International Congress of Aquaculture and Fisheries-2016
Event date： 2016.11.5
Language：English Presentation type：Oral presentation (general)
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みやざきサクラマスを用いた機能性魚醤油の開発
深谷一斗, 中村美穂，田中竜介，内田勝久，田岡洋介
CRC第23回技術・研究発表交流会 宮崎大学産学・地域連携センター
Event date： 2016.9.27 - 2017.9.27
Language：Japanese Presentation type：Oral presentation (general)