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Affiliation |
Faculty of Medicine School of Medicine Department of Anatomy, Histochemistry and Cell Biology |
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Professor |
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External Link |
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HISHIKAWA Yoshitaka
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Degree 【 display / non-display 】
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博士（医学） ( 1997.5 島根医科大学 )
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医学士 ( 1989.3 島根医科大学 )
Research Areas 【 display / non-display 】
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Life Science / Cell biology
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Life Science / Anatomy
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Life Science / Digestive surgery
Papers 【 display / non-display 】
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Choijookhuu Narantsog, Shibata Yasuaki, Ishizuka Takumi, Xu Yan, Koji Takehiko, Hishikawa Yoshitaka
ACTA HISTOCHEMICA ET CYTOCHEMICA 55 ( 5 ) 119 - 128 2022.10
Authorship：Last author, Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY
<p><i>In situ</i> hybridization (ISH) is a powerful method for detecting specific RNAs at the cellular level. Although conventional ISH using hapten-labeled probes are useful for detecting multiple RNAs, the detection procedures are still complex and required longer time. Therefore, we introduced a new application of fluorescence resonance energy transfer (FRET)-based molecular beacon (MB) probes for ISH. MCF-7 cells and C57BL/6J mouse uterus were used for ISH. MB probes for ERα mRNA and 28S rRNA were labeled with Cy3/BHQ-2 and 6-FAM/DABCYL, and conventional probes were labeled with digoxigenin. Fluorescence measurements revealed that of more-rapid hybridization kinetics compared to conventional probes. In MCF-7 cells, 28S rRNA was detected in nucleolus and cytoplasm of all cells, whereas ERα mRNA was detected in some nucleolus. In the uterus, 28S rRNA was clearly detected using complementary MB probe, but there were no signals in control slides. Moreover, 28S rRNA was detected in all cells, whereas ERα mRNA was detected mainly in the epithelium. Fluorescence intensity of 28S rRNA was decreased significantly in 1 or 2 base-mismatched sequences, that indicates highly specific detection of target RNAs. In conclusion, the FRET-based MB probes are very useful for ISH, providing rapid hybridization, high sensitivity and specificity.</p>
DOI： 10.1267/ahc.22-00075
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Crucial role of high-mobility group box 2 in mouse ovarian follicular development through estrogen receptor beta. Reviewed International journal
Yu Yamaguma, Naohiro Sugita, Narantsog Choijookhuu, Koichi Yano, Deokcheol Lee, Makoto Ikenoue, Fidya, Shinichiro Shirouzu, Takumi Ishizuka, Mio Tanaka, Yoshihiro Yamashita, Etsuo Chosa, Noboru Taniguchi, Yoshitaka Hishikawa
Histochemistry and cell biology 157 ( 3 ) 359 - 369 2022.3
Authorship：Last author, Corresponding author Language：English Publishing type：Research paper (scientific journal)
High-mobility group box 2 (HMGB2) is a chromatin-associated protein that is an important regulator of gene transcription, recombination, and repair processes. The functional importance of HMGB2 has been reported in various organs, including the testis, heart, and cartilage. However, its role in the ovary is largely unknown. In this study, ovary tissues from wild-type (WT) and HMGB2-knock-out (KO) mice were examined by histopathological staining and immunohistochemistry. The ovary size and weight were significantly lower in HMGB2-KO mice than in age-matched WT littermates. Histopathological analysis revealed ovarian atrophy and progressive fibrosis in 10-month-old HMGB2-KO mouse ovaries. Compared to age-matched WT mice, the numbers of oocytes and developing follicles were significantly decreased at 2 months of age and were completely depleted at 10 months of age in HMGB2-KO mice. Immunohistochemistry revealed the expression of HMGB2 in the granulosa cells of developing follicles, oocytes, some corpora lutea, and stromal cells. Importantly, HMGB2-positive cells were co-localized with estrogen receptor beta (ERβ), but not ERα. Estrogen response element-binding activity was demonstrated by southwestern histochemistry, and it was decreased in HMGB2-KO mouse ovaries. Cell proliferation activity was also decreased in HMGB2-KO mouse ovaries in parallel with the decreased folliculogenesis. These results indicated that the depletion of HMGB2 induced ovarian atrophy that was characterized by a decreased ovarian size and weight, progressive fibrosis, as well as decreased oocytes and folliculogenesis. In conclusion, we demonstrated the crucial role of HMGB2 in mouse ovarian folliculogenesis through ERβ expression.
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Sugita N, Choijookhuu N, Yano K, Lee D, Ikenoue M, Fidya, Taniguchi N, Chosa E, Hishikawa Y
Biology of reproduction ;105(6) 105 ( 6 ) 1510 - 1520 2021.10
Authorship：Last author, Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：Biology of Reproduction
High-mobility group box 2, a chromatin-associated protein that interacts with deoxyribonucleic acid, is implicated in multiple biological processes, including gene transcription, replication, and repair. High-mobility group box 2 is expressed in several tissues, including the testis; however, its functional role is largely unknown. Here, we elucidated the role of high-mobility group box 2 in spermatogenesis. Paraffin-embedded testicular tissues were obtained from 8-week-old and 1-year-old wild-type and knock-out mice. Testis weight and number of seminiferous tubules were decreased, whereas atrophic tubules were increased in high-mobility group box 2-depleted mice. Immunohistochemistry revealed that atrophic tubules contained Sertoli cells, but not germ cells. Moreover, decreased cell proliferation and increased apoptosis were demonstrated in high-mobility group box 2-depleted mouse testis. To elucidate the cause of tubule atrophy, we examined the expression of androgen and estrogen receptors, and the results indicated aberrant expression of androgen receptor and estrogen receptor alpha in Sertoli and Leydig cells. Southwestern histochemistry detected decreased estrogen response element–binding sites in high-mobility group box 2-depleted mouse testis. High-mobility group box 1, which has highly similar structure and function as high-mobility group box 2, was examined by immunohistochemistry and western blotting, which indicated increased expression in testis. These findings indicate a compensatory increase in high-mobility group box 1 expression in high-mobility group box 2 knock-out mouse testis. In summary, depletion of high-mobility group box 2 induced aberrant expression of androgen receptor and estrogen receptor alpha, leading to decreased germ cell proliferation and increased apoptosis which resulted in focal seminiferous tubule atrophy.
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Protective role of estrogen through G-protein coupled receptor 30 in a colitis mouse model. Reviewed
Fidya, Choijookhuu N, Ikenoue M, Yano K, Yamaguma Y, Shirouzu S, Kai K, Ishizuka T, Hishikawa Y
Histochem Cell Biol 2023.10
Authorship：Corresponding author Publishing type：Research paper (scientific journal)
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Tmem161a regulates bone formation and bone strength through the P38 MAPK pathway. Reviewed
Nagai T, Sekimoto T, Kurogi S, Ohta T, Miyazaki S, Yamaguchi Y, Tajima T, Chosa E, Imasaka M, Yoshinobu K, Araki K, Araki M, Choijookhuu N, Sato K, Hishikawa Y, Funamoto T
Sci Rep 2023.9
Authorship：Corresponding author Publishing type：Research paper (scientific journal)
Books 【 display / non-display 】
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In situ hybridization 法
菱川善隆, Narantsog Choijookhuu, 石塚匠, 柴田恭明, 小路武彦（ Role： Joint author）
日本組織細胞化学会（編），中西印刷． 2022.7
Language：Japanese Book type：Scholarly book
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病理と臨床 2022 vol.40 臨時増刊号
菱川善隆, Narantsog Choijookhuu, 石塚匠, 柴田恭明, 小路武彦（ Role： Joint author , 第1部 がんの分子病理学（序論）- B 解析法 - 1 FISH・ISH）
文光堂 2022.4
Responsible for pages：1139-1144 Language：Japanese Book type：Scholarly book
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In focus in HCB: new histochemical insights into mammalian gametogenesis
Hishikawa Y, Takizawa T, Koji T（ Role： Joint author）
Springer 2022.3
Language：English Book type：Scholarly book
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In situ hybridization法の原理と応用
菱川善隆, 柴田恭明, 小路武彦（ Role： Joint author）
中西印刷 2021.8
Language：Japanese Book type：Scholarly book
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In situ hybridization法の原理と応用
菱川善隆, 柴田恭明, 小路武彦（ Role： Joint author）
中西印刷 2020.8
Language：Japanese Book type：Scholarly book
MISC 【 display / non-display 】
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In focus in HCB: new histochemical insights into mammalian gametogenesis
Hishikawa Y., Takizawa T., Koji T.
Histochemistry and Cell Biology 157 ( 3 ) 269 - 271 2022.3
Language：English Publishing type：Article, review, commentary, editorial, etc. (scientific journal) Publisher：Histochemistry and Cell Biology
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Lee D., Taniguchi N., Sato K., Choijookhuu N., Hishikawa Y., Kataoka H., Morinaga H., Lotz M., Chosa E.
Scientific Reports 10 ( 1 ) 4647 2020.12
Language：Japanese Publishing type：Rapid communication, short report, research note, etc. (scientific journal) Publisher：Scientific Reports
The original version of this Article contained an error in Affiliation 6, which was incorrectly given as ‘Department of Internal Medicine and Bioregulatory Science, Graduate School of Medical Sciences, 3-1-1 Maidashi, Higashi, Fukuoka, 812-8582, Japan’. The correct affiliation is listed below: Department of Internal Medicine and Bioregulatory Science, Graduate School of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi, Fukuoka, 812-8582, Japan. Additionally, in the Supplementary Information file originally published with this Article, Affiliation 2 was incorrectly given as ‘Department of Medical Science, Tokyo Medical University, 6-1-1 Shinjuku, Shinjuku, Tokyo 160- 8402, Japan’. The correct affiliation is listed below: Institute of Medical Science, Tokyo Medical University, 6-1-1 Shinjuku, Shinjuku-ku, Tokyo, 160-8402, Japan These errors have now been corrected in the HTML and PDF versions of the article, alongside the Supplementary information file.
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Fukui T., Fukaya T., Uto T., Takagi H., Nasu J., Miyanaga N., Nishikawa Y., Koseki H., Choijookhuu N., Hishikawa Y., Yamashita Y., Sato K.
Scientific Reports 10 ( 1 ) 16375 2020.12
Language：Japanese Publishing type：Rapid communication, short report, research note, etc. (scientific journal) Publisher：Scientific Reports
This Article contains errors in the Methods section, under the subheading ‘Generation of Cd103-/- mice’, “The linearized targeting construct was introduced by electroporation into C57BL/6-derived JN/2 recombinant embryonic stem cell (ESC) and neomycin-resistant clones were first screened for homologous recombination by PCR utilizing a pair of the following oligonucleotides: Primer 1 (5'-ATA TGT AGT GTC TGG TCA GGA TAA TAG TTG-3') and Primer 2 (5'-ATA ACC TCC TCT CCT ATG GTA CCT AAA C-3').” should read: “The linearized targeting construct was introduced by electroporation into C57BL/6-derived JN/2 recombinant embryonic stem cell (ESC) and neomycin-resistant clones were first screened for homologous recombination by PCR utilizing a pair of the following oligonucleotides: Primer 1 (5'-ATA TGT AGT GTC TGG TCA GGA TAA TAG TTG-3') and Primer 3 (5'-ATA ACC TCC TCT CCT ATG GTA CCT AAA C-3').” “Transmission of the targeted allele was confirmed by PCR with Primer 1 and Primer 3 (5'-CTT TAT ATT TCA TTT TTG CTC AGG CTT C-3'). The mutant mice were cross-mated for more than nine generations with B6.FLIP mice to excise the flanked FRT sites by Flp-recombinase, and 8- to 12-week-old Cd103+/+ littermates were used as WT mice. Then, Cd103+/- littermates were crossed to obtain homozygotes, and transmission of the targeted allele was confirmed by PCR with Primer 1 and Primer 3.” should read: “Transmission of the targeted allele was confirmed by PCR with Primer 1 and Primer 2 (5'-CTT TAT ATT TCA TTT TTG CTC AGG CTT C-3'). The mutant mice were cross-mated for more than nine generations with B6.FLIP mice to excise the flanked FRT sites by Flp-recombinase, and 8- to 12-week-old Cd103+/+ littermates were used as WT mice. Then, Cd103+/- littermates were crossed to obtain homozygotes, and transmission of the targeted allele was confirmed by PCR with Primer 1 and Primer 2”.
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An S., Soe K., Akamatsu M., Hishikawa Y., Koji T.
Histochemistry and Cell Biology 153 ( 4 ) 287 - 288 2020.4
Language：Japanese Publishing type：Rapid communication, short report, research note, etc. (scientific journal) Publisher：Histochemistry and Cell Biology
The figure shown below is the correct version. We apologize for the mistake.
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in situ hybridizationの最前線 －技術の最前線の状況と研究・診断への応用－ Reviewed
菱川善隆, 柴田恭明, 小路武彦
病理と臨床 37 ( 11 ) 1139 - 1144 2019.11
Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (scientific journal) Publisher：文光堂
Presentations 【 display / non-display 】
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地方大学医学部における 外国人大学院生の確保と質の担保について
菱川善隆
第16回(令和5年度実施) 大学院医学研究科教育懇談会 2023.12.18
Event date： 2023.12.18
Presentation type：Public lecture, seminar, tutorial, course, or other speech
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The loss of HMGB2 causes seminiferous tubule atrophy via aberration of androgen and estrogen receptors in mouse testis.
2021.10.22
Event date： 2021.10.22 - 2021.10.24
Language：English Presentation type：Oral presentation (general)
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Depletion of HMGB2 induces aberrant expression of sex steroid hormone receptors that causes seminiferous tubule atrophy in mouse testis.
2021.9.26
Event date： 2021.9.25 - 2021.9.26
Language：English Presentation type：Oral presentation (general)
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マウス肝再生過程におけるHMGB２の肝細胞増殖制御への関与
矢野 公一, Narantsog Choijookhuu, 池ノ上 実, Fidya, 七島 篤志, 菱川 善隆
第62回日本組織細胞化学会総会・学術集会 (web開催) 2021.9.26 日本組織細胞化学会
Event date： 2021.9.25 - 2021.9.26
Language：Japanese Presentation type：Oral presentation (general)
Venue：web開催
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蛍光共鳴エネルギー移動 （FRET)を利用したin situ hybridizationの実際
菱川 善隆, Narantsog Choijookhuu, 石塚 匠, 柴田 恭明, 小路 武彦
第62回日本組織細胞化学会総会・学術集会 (web開催) 2021.9.25 日本組織細胞化学会
Event date： 2021.9.25 - 2021.9.26
Language：Japanese Presentation type：Oral presentation (general)
Venue：web開催
Works 【 display / non-display 】
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Assessment and possible implications in multiple infections and their related cancers in Myanmar
2016.1
ミャンマー国保健省医学研究局と宮崎大学との保健医療関連分野におけるシンポジウムに参加した。
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Kick-Off Symposium on Promoting Environmental Health in Arsenic Contaminated Area in Myanmar
2015.8
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The9th International Conference on Genetic and Evolutionary Computing(ICGEC 2015)
2015.8
最新の技術について情報を得た。
Awards 【 display / non-display 】
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2020 年度（第 22 回）日本組織細胞化学会論文賞
2020.12 日本組織細胞化学会 The HDAC Inhibitor, SAHA, Combined with Cisplatin Synergistically Induces Apoptosis in Alpha-fetoprotein-producing Hepatoid Adenocarcinoma Cells
Kyaw MTH, Yamaguchi Y, Choijookhuu N, Yano K, Takagi H, Takahashi N, Oo PS, Sato K, Hishikawa Y
Award type：Honored in official journal of a scientific society, scientific journal Country：Japan
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2020 年度（第 22 回）日本組織細胞化学会論文賞
2020.12 日本組織細胞化学会 Estrogen Regulates Mitochondrial Morphology through Phosphorylation of Dynamin-related Protein 1 in MCF7 Human Breast Cancer Cells
Phyu Synn Oo, Yuya Yamaguchi, Akira Sawaguchi, Myat Tin Htwe Kyaw, Narantsog Choijookhuu, Mohmand Noor Ali, Naparee Srisowanna, Shin-ichiro Hino, Yoshitaka Hishikawa
Award type：Honored in official journal of a scientific society, scientific journal Country：Japan
Grant-in-Aid for Scientific Research 【 display / non-display 】
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クリック反応技術と軸配位子糖鎖連結ポリフィリン錯体を融合した革新的PDTの開発
Grant number：22K08806 2022.04 - 2025.03
独立行政法人日本学術振興会 科学研究費補助金 基盤研究(C)
七島 篤志、
Authorship：Coinvestigator(s)
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脂肪肝マウスの肝再生過程でのミトコンドリア脂質代謝へのエストロゲンの関与
Grant number：21K06738 2021.04 - 2024.03
独立行政法人日本学術振興会 科学研究費補助金 基盤研究(C)
Authorship：Principal investigator
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ミャンマーヒ素汚染地域での発達、発育に関するコホート調査
Grant number：18KK0267 2018.04 - 2024.03
独立行政法人日本学術振興会 科学研究費補助金 国際共同研究加速基金(国際共同研究強化(B))
黒田 嘉紀
Authorship：Coinvestigator(s)
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高感度蛍光in situハイブリダイゼーション法によるmicroRNA発現解析
2016.04 - 2019.03
科学研究費補助金 基盤研究(C)
Authorship：Principal investigator
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肝再生過程でのエストロゲン受容体によるミトコンドリア形態変化と機能制御の解明
2012.04 - 2015.03
科学研究費補助金 基盤研究(C)
Authorship：Principal investigator
肝再生過程でのエストロゲン受容体によるミトコンドリア形態変化と機能制御の解明
Other research activities 【 display / non-display 】
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第46回組織細胞化学講習会
2021.08
「In situ hybridization法の原理と応用 」講演をオンラインで行った。
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第45回組織細胞化学講習会
2020.08
「In situ hybridization法の原理と応用 」講演をオンラインで行った。
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第44回組織細胞化学講習会
2019.08
「In situ hybridization (RNA)の基礎と応用 」講演を行った。
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第43回組織細胞化学講習会(技術）
2019.08
「In situハイブリダイゼーション法の実践 」wet lab を行った。
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第124回日本解剖学会総会・全国学術集会
2019.03
解剖学の最新の研究成果について情報交換を行った。