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Faculty of Medicine School of Medicine Department of Anatomy, Histochemistry and Cell Biology |
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HISHIKAWA Yoshitaka
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Degree 【 display / non-display 】
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博士（医学） ( 1997.5 島根医科大学 )
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医学士 ( 1989.3 島根医科大学 )
Research Areas 【 display / non-display 】
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Life Science / Cell biology
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Life Science / Anatomy
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Life Science / Digestive surgery
Papers 【 display / non-display 】
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Fidya, Ishizuka Takumi, Kubota Toshiki, Kai Kengo, Hishikawa Yoshitaka
ACTA HISTOCHEMICA ET CYTOCHEMICA 59 ( 2 ) 89 - 99 2026.4
Authorship：Last author Language：English Publishing type：Research paper (scientific journal) Publisher：JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY
Periodontitis is a chronic inflammatory disease characterized by collagen degradation and alveolar bone loss. Although estrogen contributes to periodontal homeostasis, the role of the membrane-bound G protein–coupled estrogen receptor 30 (GPR30) in periodontitis remains unclear. This study investigated the temporal involvement of GPR30 in ligature-induced periodontitis, focusing on alveolar bone changes, collagen integrity, fibroblast activation, and epigenetic regulation. Twenty male Wistar rats were allocated to control and periodontitis groups and evaluated at days 7, 14, and 21 following placements of a stainless-steel ligature around the maxillary first molars. Alveolar bone loss was assessed radiographically, while inflammatory changes and collagen organization were examined using hematoxylin–eosin and Masson’s trichrome staining. Immunohistochemistry was performed to evaluate GPR30, collagen I/III, α-smooth muscle actin (α-SMA), and histone modifications. Statistical analysis was conducted using one-way ANOVA with Tukey’s post hoc test. Alveolar bone loss and collagen degradation were detectable on day 7 and peaked at day 14. GPR30 expression increased during the active inflammatory phase, accompanied by elevated α-SMA and histone H4 lysine 8 crotonylation (H4K8cr), and declined by day 21. Fibroblast transiently adopted a myofibroblast phenotype during inflammation and regressed during early tissue repair. Temporal changes in H4K8cr closely paralleled GPR30 expression. These findings indicate that GPR30 is dynamically associated with inflammatory and remodeling phases of periodontitis and may contribute to epigenetic regulation during periodontal tissue remodeling.
DOI： 10.1267/ahc.26-00002
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Non-Cytotoxic Photodynamic Therapy with Talaporfin Sodium Reduces the Expression of CXCR4 and Enhances Chemotherapeutic Efficacy in Undifferentiated Gastric Cancer Cell Line HGC27. Reviewed
Kengo Kai, Takumi Ishizuka, Jin Matsumoto, Koki Shimamawari, Ryoma Mori, Fidya, Baljinnyam Lkham-Erdene, Toshiki Kubota, Makoto Ikenoue, Kazuhiro Higuchi, Atsushi Nanashima, Yoshitaka Hishikawa
Acta Histochem. Cytochem 2025.4
Authorship：Corresponding author Publishing type：Research paper (scientific journal)
DOI： 10.1267/ahc.25-00002
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Higuchi Kazuhiro, Ikenoue Makoto, Ishizuka Takumi, Kai Kengo, Takahashi Nobuyasu, Kubota Toshiki, Shirouzu Shinichiro, Lkham-Erdene Baljinnyam, Aung Kham Mo, Nakai Michikazu, Sawaguchi Akira, Nanashima Atsushi, Hishikawa Yoshitaka
ACTA HISTOCHEMICA ET CYTOCHEMICA 58 ( 1 ) 9 - 18 2025.2
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY
SET domain bifurcated 1 (SETDB1), a histone H3K9-specific methyltransferase, is crucial for heterochromatin formation and intestinal homeostasis, but its role in intestinal ischemia-reperfusion injury (IRI) remains unclear. This study investigated changes in SETDB1-mediated nuclear chromatin regulation in intestinal epithelial cells (IECs) using an IRI mouse model. Jejunal samples were collected after 75 min of ischemia followed by 24 hr of reperfusion. Sinefungin was administered as a histone methyltransferase inhibitor. Morphologic changes were evaluated using hematoxylin-eosin staining and electron microscopy, and cell-adhesion molecule expression, including ZO-1, E-cadherin, integrin-β4, and laminin, was evaluated using immunohistochemistry. Super-resolution microscopy analyzed intranuclear SETDB1 localization and heterochromatin formation in IECs. IRI-affected jejunum exhibited massive IEC detachment, dilated intercellular spaces, basement membrane damage, and decreased expression of E-cadherin and integrin-β4. Sinefungin prevented these changes, however. The proportion of IECs expressing nuclear SETDB1 throughout the euchromatin was significantly higher in IRI-affected jejunum (77.8%) than sham-treated (3.0%) or sinefungin-treated, IRI-affected jejunum (2.7%). The proportion of IECs with decreased heterochromatin was significantly higher in sinefungin-treated, IRI-affected jejunum (84.3%) than untreated IRI-affected jejunum (15.6%). These findings suggest that SETDB1-mediated chromatin regulation is pivotal in intestinal IRI and represents a potential therapeutic target.
DOI： 10.1267/ahc.24-00061
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Lkham-Erdene Baljinnyam, Choijookhuu Narantsog, Kubota Toshiki, Uto Tomofumi, Mitoma Shuya, Ishizuka Takumi, Kai Kengo, Shirouzu Shinichiro, Higuchi Kazuhiro, Mo Aung Kham, Batmunkh Jargal-Erdene, Sato Katsuaki, Hishikawa Yoshitaka
ACTA HISTOCHEMICA ET CYTOCHEMICA 57 ( 5 ) 175 - 188 2024.10
Authorship：Last author, Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY
Metabolic dysfunction-associated steatotic liver disease (MASLD) is becoming a major health problem worldwide. Liver regeneration is crucial for restoring liver function, and is regulated by extraordinary complex process, involving numerous factors under both physiologic and pathologic conditions. Sphingosine-1-phosphate (S1P), a bioactive sphingolipid synthesized by sphingosine kinase 1 (SphK1), plays an important role in liver function through S1P receptors (S1PRs)-expressing cells. In this study, we investigated the effect of lipid overload on hepatocyte proliferation in a mouse hepatic steatosis model induced by feeding a methionine- and choline-deficient (MCD) diet. After 50% partial hepatectomy (PHx), liver tissues were sampled at various timepoints and then analyzed by immunohistochemistry, oil Red-O staining, quantitative-polymerase chain reaction (qPCR), and flow cytometry. In mice fed the MCD-diet, significantly exacerbated hepatic steatosis and accelerated liver regeneration were observed. After PHx, hepatocyte proliferation peaked at 48 and 36 hr in the liver of chow- and MCD-diet fed mice, respectively. By contrast, increased expression of S1PR2 was observed in hepatic neutrophils and macrophages of MCD-diet fed mice. Flow cytometry and qPCR experiments demonstrated that levels of HGF and FGF2 released by neutrophils and macrophages were significantly higher in MCD-diet fed mice. In conclusion, hepatic lipid overload recruits Kupffer cells and neutrophils that release HGF and FGF2 via SphK1/S1PR2 activation to accelerate hepatocyte proliferation.
DOI： 10.1267/ahc.24-00046
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Acceptance of Virtual Reality Simulation Training for Stoma Care by Healthcare Providers: A Pilot Questionnaire Study After Viewing Prototype Imagings. Reviewed
Kai K, Shinoda H, Takeiri E, Hamada T, Chikubu M, Kodama Y, Higuchi K, Nanashima A
Cureus 2024.7
Authorship：Corresponding author Publishing type：Research paper (scientific journal)
DOI： 10.7759/cureus.65465
Books 【 display / non-display 】
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組織細胞化学2023「In situ hybridization法」
菱川 善隆, チョウジョウフ ナランツオツク（ Role： Sole author）
日本組織細胞化学会(編), 中西印刷 2023
Total pages：11 Book type：Scholarly book
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In situ hybridization 法
菱川善隆, Narantsog Choijookhuu, 石塚匠, 柴田恭明, 小路武彦（ Role： Joint author）
日本組織細胞化学会（編），中西印刷． 2022.7
Language：Japanese Book type：Scholarly book
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病理と臨床 2022 vol.40 臨時増刊号
菱川善隆, Narantsog Choijookhuu, 石塚匠, 柴田恭明, 小路武彦（ Role： Joint author , 第1部 がんの分子病理学（序論）- B 解析法 - 1 FISH・ISH）
文光堂 2022.4
Responsible for pages：1139-1144 Language：Japanese Book type：Scholarly book
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In focus in HCB: new histochemical insights into mammalian gametogenesis
Hishikawa Y, Takizawa T, Koji T（ Role： Joint author）
Springer 2022.3
Language：English Book type：Scholarly book
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In situ hybridization法
菱川 善隆, チョウジョウフ ナランツオツク（ Role： Sole author）
中西印刷 2022
Total pages：258 Book type：Scholarly book
MISC 【 display / non-display 】
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In focus in HCB: new histochemical insights into mammalian gametogenesis
Hishikawa Y., Takizawa T., Koji T.
Histochemistry and Cell Biology 157 ( 3 ) 269 - 271 2022.3
Language：English Publishing type：Article, review, commentary, editorial, etc. (scientific journal) Publisher：Histochemistry and Cell Biology
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Choijookhuu N., Shibata Y., Ishizuka T., Xu Y., Koji T., Hishikawa Y.
Acta Histochemica et Cytochemica 55 ( 6 ) 2022
Publishing type：Rapid communication, short report, research note, etc. (scientific journal) Publisher：Acta Histochemica et Cytochemica
The authors would like to make the following correction: In Figure 1 on page 122, the loop part of sequence was incorrectly shown. The corrected figure is as follows. This correction to Figure 1 does not change the scientific conclusions of the article in any way.
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Lee D., Taniguchi N., Sato K., Choijookhuu N., Hishikawa Y., Kataoka H., Morinaga H., Lotz M., Chosa E.
Scientific Reports 10 ( 1 ) 4647 2020.12
Language：Japanese Publishing type：Rapid communication, short report, research note, etc. (scientific journal) Publisher：Scientific Reports
The original version of this Article contained an error in Affiliation 6, which was incorrectly given as ‘Department of Internal Medicine and Bioregulatory Science, Graduate School of Medical Sciences, 3-1-1 Maidashi, Higashi, Fukuoka, 812-8582, Japan’. The correct affiliation is listed below: Department of Internal Medicine and Bioregulatory Science, Graduate School of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi, Fukuoka, 812-8582, Japan. Additionally, in the Supplementary Information file originally published with this Article, Affiliation 2 was incorrectly given as ‘Department of Medical Science, Tokyo Medical University, 6-1-1 Shinjuku, Shinjuku, Tokyo 160- 8402, Japan’. The correct affiliation is listed below: Institute of Medical Science, Tokyo Medical University, 6-1-1 Shinjuku, Shinjuku-ku, Tokyo, 160-8402, Japan These errors have now been corrected in the HTML and PDF versions of the article, alongside the Supplementary information file.
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Fukui T., Fukaya T., Uto T., Takagi H., Nasu J., Miyanaga N., Nishikawa Y., Koseki H., Choijookhuu N., Hishikawa Y., Yamashita Y., Sato K.
Scientific Reports 10 ( 1 ) 16375 2020.12
Language：Japanese Publishing type：Rapid communication, short report, research note, etc. (scientific journal) Publisher：Scientific Reports
This Article contains errors in the Methods section, under the subheading ‘Generation of Cd103-/- mice’, “The linearized targeting construct was introduced by electroporation into C57BL/6-derived JN/2 recombinant embryonic stem cell (ESC) and neomycin-resistant clones were first screened for homologous recombination by PCR utilizing a pair of the following oligonucleotides: Primer 1 (5'-ATA TGT AGT GTC TGG TCA GGA TAA TAG TTG-3') and Primer 2 (5'-ATA ACC TCC TCT CCT ATG GTA CCT AAA C-3').” should read: “The linearized targeting construct was introduced by electroporation into C57BL/6-derived JN/2 recombinant embryonic stem cell (ESC) and neomycin-resistant clones were first screened for homologous recombination by PCR utilizing a pair of the following oligonucleotides: Primer 1 (5'-ATA TGT AGT GTC TGG TCA GGA TAA TAG TTG-3') and Primer 3 (5'-ATA ACC TCC TCT CCT ATG GTA CCT AAA C-3').” “Transmission of the targeted allele was confirmed by PCR with Primer 1 and Primer 3 (5'-CTT TAT ATT TCA TTT TTG CTC AGG CTT C-3'). The mutant mice were cross-mated for more than nine generations with B6.FLIP mice to excise the flanked FRT sites by Flp-recombinase, and 8- to 12-week-old Cd103+/+ littermates were used as WT mice. Then, Cd103+/- littermates were crossed to obtain homozygotes, and transmission of the targeted allele was confirmed by PCR with Primer 1 and Primer 3.” should read: “Transmission of the targeted allele was confirmed by PCR with Primer 1 and Primer 2 (5'-CTT TAT ATT TCA TTT TTG CTC AGG CTT C-3'). The mutant mice were cross-mated for more than nine generations with B6.FLIP mice to excise the flanked FRT sites by Flp-recombinase, and 8- to 12-week-old Cd103+/+ littermates were used as WT mice. Then, Cd103+/- littermates were crossed to obtain homozygotes, and transmission of the targeted allele was confirmed by PCR with Primer 1 and Primer 2”.
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An S., Soe K., Akamatsu M., Hishikawa Y., Koji T.
Histochemistry and Cell Biology 153 ( 4 ) 287 - 288 2020.4
Language：Japanese Publishing type：Rapid communication, short report, research note, etc. (scientific journal) Publisher：Histochemistry and Cell Biology
The figure shown below is the correct version. We apologize for the mistake.
Presentations 【 display / non-display 】
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マウス肝再生過程におけるDNA四重鎖構造の時空間的形成
石塚 匠, Kham Mo Aung, 久保田 壽樹, Lkham-Erdene Baljinnyam, 甲斐 健吾, Phyu Synn Oo, 菱川 善隆
第131回 日本解剖学会総会・全国学術集会 2026.3.24
Event date： 2026.3.24 - 2026.3.26
Presentation type：Oral presentation (general)
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Mitochondria dynamin-related protein Drp1 and pDrp1-Ser616 as potential biomarkers in ER-positive breast cancer in Malaysian women
Oo Phyu Synn, Leong Wen Kang , Mohammad Hatta Nur Fatehah Binti , Aye Lwin Mie , Shamsudin Noor Hasni , Win Thin Thin , Takumi Ishizuka, Kengo Kai, Yoshitaka Hishikawa
第131回 日本解剖学会総会・全国学術集会 2026.3.25
Event date： 2026.3.24 - 2026.3.26
Presentation type：Oral presentation (general)
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Role of G-quadruplex DNA structures during mouse spermatogenesis
Aung Kham Mo, Takumi Ishizuka, Ryonosuke Kai, Lkham-Erdene Baljinnyam, Toshiki Kubota, Kengo Kai, Oo Phyu Synn, Yoshitaka Hishikawa
第131回 日本解剖学会総会・全国学術集会 2026.3.24
Event date： 2026.3.24 - 2026.3.26
Presentation type：Oral presentation (general)
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分子組織細胞化学の最前線 ～超解像顕微鏡で探るタンパク質・遺伝子発現動態～
菱川 善隆
第58回NPO法人日本口腔科学会九州地方部会 (宮崎) 2025.12.6
Event date： 2025.12.6
Language：Japanese Presentation type：Public lecture, seminar, tutorial, course, or other speech
Venue：宮崎
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蛍光共鳴エネルギー移動(FRET)を利用した in situ hybridization 法
甲斐 健吾, 石塚 匠 , 菱川 善隆
第57回日本臨床分子形態学総会・学術集会 (北九州国際会議場) 2025.11.14
Event date： 2025.11.14 - 2025.11.15
Language：Japanese Presentation type：Symposium, workshop panel (public)
Venue：北九州国際会議場
Works 【 display / non-display 】
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Assessment and possible implications in multiple infections and their related cancers in Myanmar
2016.1
ミャンマー国保健省医学研究局と宮崎大学との保健医療関連分野におけるシンポジウムに参加した。
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Kick-Off Symposium on Promoting Environmental Health in Arsenic Contaminated Area in Myanmar
2015.8
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The9th International Conference on Genetic and Evolutionary Computing(ICGEC 2015)
2015.8
最新の技術について情報を得た。
Awards 【 display / non-display 】
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2023 年度（第 25回）日本組織細胞化学会論文賞
2023.10 日本組織細胞化学会 An Advanced Detection System for In Situ Hybridization Using a Fluorescence Resonance Energy Transfer-based Molecular Beacon Probe
Choijookhuu N, Shibata Y, Ishizuka T, Xu Y, Koji T, Hishikawa Y
Award type：Honored in official journal of a scientific society, scientific journal Country：Japan
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2020 年度（第 22 回）日本組織細胞化学会論文賞
2020.12 日本組織細胞化学会 The HDAC Inhibitor, SAHA, Combined with Cisplatin Synergistically Induces Apoptosis in Alpha-fetoprotein-producing Hepatoid Adenocarcinoma Cells
Kyaw MTH, Yamaguchi Y, Choijookhuu N, Yano K, Takagi H, Takahashi N, Oo PS, Sato K, Hishikawa Y
Award type：Honored in official journal of a scientific society, scientific journal Country：Japan
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2020 年度（第 22 回）日本組織細胞化学会論文賞
2020.12 日本組織細胞化学会 Estrogen Regulates Mitochondrial Morphology through Phosphorylation of Dynamin-related Protein 1 in MCF7 Human Breast Cancer Cells
Phyu Synn Oo, Yuya Yamaguchi, Akira Sawaguchi, Myat Tin Htwe Kyaw, Narantsog Choijookhuu, Mohmand Noor Ali, Naparee Srisowanna, Shin-ichiro Hino, Yoshitaka Hishikawa
Award type：Honored in official journal of a scientific society, scientific journal Country：Japan
Grant-in-Aid for Scientific Research 【 display / non-display 】
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MASHマウス肝再生過程でのエストロゲンによる細胞増殖・脂質代謝の分子制御機構の解明
Grant number：25K10146 2025.04 - 2028.03
独立行政法人日本学術振興会 科学研究費基金 基盤研究(C)
Authorship：Principal investigator
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PDP効果に基づく胃癌腹膜播種に対する簡便かつ安全な光治療の開発
Grant number：24K11937 2024.04 - 2027.03
独立行政法人日本学術振興会 科学研究費基金 基盤研究(C)
Authorship：Coinvestigator(s)
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クリック反応技術と軸配位子糖鎖連結ポリフィリン錯体を融合した革新的PDTの開発
Grant number：22K08806 2022.04 - 2025.03
独立行政法人日本学術振興会 科学研究費補助金 基盤研究(C)
七島 篤志、
Authorship：Coinvestigator(s)
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脂肪肝マウスの肝再生過程でのミトコンドリア脂質代謝へのエストロゲンの関与
Grant number：21K06738 2021.04 - 2024.03
独立行政法人日本学術振興会 科学研究費補助金 基盤研究(C)
Authorship：Principal investigator
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ミャンマーヒ素汚染地域での発達、発育に関するコホート調査
Grant number：18KK0267 2018.04 - 2024.03
独立行政法人日本学術振興会 科学研究費補助金 国際共同研究加速基金(国際共同研究強化(B))
黒田 嘉紀
Authorship：Coinvestigator(s)
Other research activities 【 display / non-display 】
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第48回組織細胞化学講習会
2023.08
「In situ hybridization法の基礎」の講演を行った。
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第47回組織細胞化学講習会
2022.08
「In situ hybridization法」の講演を行った。
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第46回組織細胞化学講習会
2021.08
「In situ hybridization法の原理と応用 」講演をオンラインで行った。
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第45回組織細胞化学講習会
2020.08
「In situ hybridization法の原理と応用 」講演をオンラインで行った。
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第43回組織細胞化学講習会(技術）
2019.08
「In situハイブリダイゼーション法の実践 」wet lab を行った。