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Affiliation |
Faculty of Agriculture Region of Veterinary Science |
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Associate Professor |
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Related SDGs |
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WAKITANI Shoichi
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Papers 【 display / non-display 】
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Tsuji E., Sumi K., Tsuzuki N., Kondoh D., Tsujio M., Hosotani M., Namba T., Wakitani S., Ichii O., Nakanishi K., Nakamura T.
Microscopy and Microanalysis 31 ( 4 ) 2025.8
Language：English Publishing type：Research paper (scientific journal) Publisher：Microscopy and Microanalysis
Histological staining is essential for understanding bone structure and pathology; however, variations in decalcification agents can compromise reproducibility. We have previously developed a novel osteochondral staining method, Join of the Five dyes Revealing coLlagenous tissue (JFRL) staining, that is independent of the decalcification method. To promote its widespread adoption, this study confirms the robustness of JFRL staining through intra- and inter-laboratory validation. JFRL staining demonstrated consistent patterns across different manufacturers and facilities, with proper dehydration steps being crucial for optimal results. We applied JFRL staining to diverse vertebrate species prepared under various fixation and decalcification conditions to effectively visualize species-specific bone structures, including distinct osteoid and mineralized bone features from fish to large mammals. Furthermore, JFRL staining proved useful in evaluating bone biomaterials within defect models and clearly depicts the complex architecture of bone-healing processes and material integration. The staining qualitatively distinguished osteoid, mineralized bone, hyaline cartilage, and bone cells of different colors across all applications. These findings establish JFRL staining as a robust and versatile method for bone histology. Future studies focusing on quantitative assessment and pathological applications will prove that JFRL staining presents a reliable tool for both basic research and clinical diagnostics of bone disorders.
DOI： 10.1093/mam/ozaf058
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WAKITANI Shoichi, KAWABATA Risako, SHIRATAKI Sora, YASUDA Masahiro
Journal of Veterinary Medical Science 87 ( 2 ) 131 - 134 2025
Authorship：Lead author, Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：JAPANESE SOCIETY OF VETERINARY SCIENCE
Immunohistochemistry for keratins 5, 8, 14, and 18 was performed on Japanese Black calf thymuses at various stages of acute thymic involution. Keratins 5 and 14 were predominantly localized in the thymic medulla, while keratins 8 and 18 were broadly distributed throughout the parenchyma. Despite thymic involution, the distribution patterns of these keratins remained consistent. The cortical area, assessed by keratin 5 staining, progressively decreased with involution but retained approximately 40% of the total parenchyma even at the most severe stage. These results suggest that the thymic cortex shrinks but does not completely disappear during acute thymic involution in calves.
DOI： 10.1292/jvms.24-0413
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Furukawa R., Wakitani S., Kawabata R., Yasuda M.
Veterinary Journal 307 106225 2024.10
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：Veterinary Journal
Transcriptome analysis was performed on the thymus of Japanese Black calves that were necropsied due to poor prognosis, to characterize changes associated with acute thymic involution. Gene expression profiles obtained by DNA microarray analysis of eight calf thymuses were classified into three patterns that correlated with the histopathological stage of acute thymic involution. Using principal component analysis, the first principal component of the global gene expression levels in the calf thymus was associated with the stage of acute thymic involution, suggesting that histopathological changes greatly influence the gene expression profile. Gene ontology enrichment analysis revealed that genes related to cell proliferation, wound healing, and inflammatory responses were the main contributors to the first principal component. Real-time RT-PCR showed that the thymus had lower expression of PCNA, KIFC1, and HES6, and higher expression of SYNPO2, PDGFRB, and TWIST1 during acute thymic involution. Immunohistochemistry demonstrated a decrease in the rate of Ki67-positive cells in the thymic cortex during the late stage of acute thymic involution. The rate of cleaved caspase-1-positive cells increased in the thymic cortex at an earlier stage than the increase in the rate of cleaved caspase-3-positive cells. Vimentin, which was almost absent in the non-involuted thymic cortex, appeared in the thymic cortex during acute thymic involution. These results suggest that in farmed calves with a poor prognosis, inflammatory responses and impaired thymocyte proliferation are primarily involved in acute thymic involution.
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Anatomical View of the Internal Carotid Artery Occlusion in Japanese Black Cattle Reviewed
Jadi Arvendi Rachma, Fujisaki Hinako, Ramah Amany, Baakhtari Mahmoud, Imatake Shoichiro, Wakitani Shoichi, Yasuda Masahiro
Animals 14 ( 3 ) 365 - 365 2024.2
Language：English Publishing type：Research paper (scientific journal)
The internal carotid artery (ICA) is a branch of the common carotid artery (CCA), along with the external carotid artery (ECA), which together provide the blood supply for the brain. The description of the ICA in cattle is vague, including denial of its existence or degeneration at an early stage after birth. This anatomical study investigated the internal carotid artery in Japanese black cattle. Sixty-five heads of Japanese black cattle aged from newborn to 13 years were dissected and injected with colored latex from the CCA after separating the head and body. Diameter measurements of the artery branches from the CCA on its bifurcation were conducted. Furthermore, a histological examination of the ICA wall’s structures, which consist of the tunica intima, tunica media, and tunica externa, was performed. The ICA of Japanese black cattle is closed on the left side after age 3 years, except for a small lumen at 13 years, whereas the right ICA remains open at all ages. The location of occlusion of the left internal carotid artery (LICA) shows thickness of the tunica intima and an increased connective tissue layer area. The diameter of the ICA does not differ between the left and right sides, and there is no correlation with age. Therefore, further studies are needed, especially of ICA occlusion related to Japanese black cattle’s physiology or cerebrospinal disease.
DOI： 10.3390/ani14030365
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Nakanishi Tomonori, Suga Keisuke, Wakitani Shoichi, Yamaguchi Kohta, Sugamoto Kazuhiro, Erickson Laurie, Kawahara Satoshi
European Journal of Lipid Science and Technology 126 ( 4 ) 2024
Language：English Publishing type：Research paper (scientific journal)
Phytanic acid is one of the branched-chain fatty acids in the human diet. Humans incorporate it into their bodies through the ingestion of ruminant meat and milk since these animal products contain the 3RS, 7R, 11R-isomer, as naturally occurring phytanic acid (nPA). Although phytanic acid has been reported to have health-promoting effects such as immunomodulatory effects, these findings were obtained mostly from in vitro cell-based experiments. In this study, the in vivo anti-inflammatory effects of nPA were investigated using a mouse model of dextran sulfate sodium (DSS)-induced colitis. Mice were fed diets containing 0%, 0.05%, or 0.1% nPA for 4 weeks. During the fourth week, acute colitis was induced by administration of a 3% DSS solution. The intake of 0.05% and 0.1% nPA inhibited DSS-induced body weight loss in mice, and showed improvement of histopathological changes in colon epithelium. nPA also inhibited the production of proinflammatory cytokines, especially macrophage-related cytokines such as interleukin-1β in the colon. These results suggest that nPA exerts anti-inflammatory effects under in vivo physiological conditions.
Practical application: There is a growing demand for fatty acids possessing distinct nutritional and physical characteristics in the realms of health, nutrition, and food-related applications. The present study demonstrated that a branched-chain fatty acid, phytanic acid, exerts anti-inflammatory activity under not only in vitro but also in vivo conditions, using a commonly used mouse model of human inflammatory bowel disease. Our findings encourage further studies to determine whether phytanic acid elicits beneficial properties in humans, and suggest that phytanic acid enrichment can be a novel product strategy in the food market.
Books 【 display / non-display 】
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獣医組織学 第8版
日本獣医解剖学会（ Role： Contributor , 第14章 心臓血管系）
学窓社 2020.3
Responsible for pages：141-153 Language：Japanese Book type：Textbook, survey, introduction
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平成31年度・令和元年度畜産生産に関する研究調査成果報告書
脇谷 晶一, 保田 昌宏（ Role： Joint author）
一般財団法人 畜産ニューテック協会 2020
Language：Japanese Book type：Report
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獣医発生学 第2版
T. A. McGeady, P. J. Quinn, E. S. Fitzpatrick, M. T. Ryan, D. Kilroy, P. Lonergan（ Role： Joint translator , 第14章 心臓血管系）
学窓社 2019.2
Responsible for pages：141-153 Language：Japanese Book type：Textbook, survey, introduction
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平成29年度食肉に関する助成研究調査成果報告書
脇谷 晶一, 保田 昌宏（ Role： Joint author）
公益財団法人 伊藤記念財団 2018.11
Language：Japanese Book type：Report
MISC 【 display / non-display 】
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TGF-β superfamily signaling in testis formation and early male germline development
Julia C. Young, Shoichi Wakitani, Kate L. Loveland
Seminars in Cell & Developmental Biology 2015.10
Language：English Publishing type：Article, review, commentary, editorial, etc. (scientific journal) Publisher：Elsevier Ltd.
Presentations 【 display / non-display 】
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牛伝染性リンパ腫細胞株に対する抗牛CD20モノクローナル抗体の機能評価
三苫 修也、宇都 倫史、深谷 知宏、山之口 奈緒、脇谷 昌一、北原 豪、関口 敏、 保田 昌宏、佐藤 克明
第168回日本獣医学会学術集会 2025.9.4
Event date： 2025.9.3 - 2025.9.6
Language：Japanese Presentation type：Oral presentation (general)
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黒毛和種廃用子牛における胸腺病的退縮に伴ったマスト細胞密度の増加
白瀧 空、脇谷 晶一、川端 理紗子、奥原 千紗貴、保田 昌宏
第168回日本獣医学会学術集会 2025.9.4
Event date： 2025.9.3 - 2025.9.6
Language：Japanese Presentation type：Oral presentation (general)
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豚の副腎における動脈分布の解剖学的破格 International coauthorship
豊﨑 祐介、Arvendi Jadi、脇谷 晶一、保田 昌宏
第168回日本獣医学会学術集会 2025.9.3
Event date： 2025.9.3 - 2025.9.6
Language：Japanese Presentation type：Oral presentation (general)
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マウス脱落膜形成予定領域におけるニューロフィラメント中鎖の先行発現
久保寺 雪乃、脇谷 晶一、保田 昌宏
第168回日本獣医学会学術集会 2025.9.3
Event date： 2025.9.3 - 2025.9.6
Language：Japanese Presentation type：Oral presentation (general)
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ウシIARS異常症モデルマウス胸腺におけるLPS誘導性アポトーシスの亢進
奥原 千紗貴、脇谷 晶一、渡邉 正輝、岡村 匡史、佐々木 宣哉、保田 昌宏
第167回日本獣医学会学術集会 2024.9.11
Event date： 2024.9.10 - 2024.9.13
Language：Japanese Presentation type：Oral presentation (general)
Grant-in-Aid for Scientific Research 【 display / non-display 】
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虚弱子牛症候群の低免疫状態に関与する胸腺皮質上皮細胞の特性解析
Grant number：22K05988 2022.04 - 2025.03
独立行政法人日本学術振興会 科学研究費基金 基盤研究(C)
Authorship：Principal investigator
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ヒトiPS細胞のFGF依存性機構の解明：Nodalシグナリングの多重制御
Grant number：16K21243 2016.04 - 2018.03
独立行政法人日本学術振興会 科学研究費補助金 若手研究(B)
Authorship：Principal investigator
ヒトiPS細胞のFGF依存性機構の解明：Nodalシグナリングの多重制御