脇谷 晶一 (ワキタニ ショウイチ)

WAKITANI Shoichi

写真a

所属

農学部 獣医学科

職名

講師

外部リンク

学位 【 表示 / 非表示

  • 博士(獣医学) ( 2011年9月   山口大学 )

 

論文 【 表示 / 非表示

  • The FGF receptor inhibitor PD173074 modulates Lefty expression in human induced pluripotent stem cells differently depending on the culture conditions 査読あり

    Wakitani S.

    Biochimica et Biophysica Acta - Molecular Cell Research   1869 ( 7 )   119260   2022年7月

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    担当区分:筆頭著者, 責任著者   記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Biochimica et Biophysica Acta - Molecular Cell Research  

    DOI: 10.1016/j.bbamcr.2022.119260

    Scopus

    PubMed

  • Insufficiency of CD205-positive cortical thymic epithelial cells in immature Japanese Black cattle with severe thymic abnormalities and poor prognosis 査読あり

    Wakitani S., Kawabata R., Yasuda M.

    Veterinary Immunology and Immunopathology   245   110379   2022年3月

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    担当区分:筆頭著者, 責任著者   記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Veterinary Immunology and Immunopathology  

    To investigate the involvement of thymic function in the development of diseases with poor prognosis in calves, this study conducted a survey for the assessment of thymus cell composition in immature Japanese Black cattle with poor prognosis. Histopathological evaluation of 47 cattle showed signs of acute thymic involution in most cases. Less than half of the cases had a cortex predominant over the medulla in the thymic parenchyma, and a quarter of the cases indicated severe histological condition with an unclear boundary between the cortex and medulla. Correlation analysis revealed a close relationship between the corresponding stages of acute involution, cortical occupancy, and the expression of CD4, CD8B, and CD205. When cases were grouped by cortical occupancy, the expression of CD4 and CD8B expression was lower in the severe group with less than 25 % cortical occupancy, and the expression of CD205 was lower in the group with an unclear cortical-medullary boundary. Meanwhile, there was no difference in the expressions of IL7, CD80, FEZF2, and FOXN1 according to cortical occupancy. Immunohistochemistry has shown that cytokeratin-positive thymic epithelial cells are more densely populated in the severe thymus. UEA-I-binding medullary thymic epithelial cells were also present, but CD205-positive cortical thymic epithelial cells were rare in severe thymus. Moreover, there were significantly fewer Ki-67-positive cells in cattle with severe thymus. Therefore, these results indicate that thymic histological abnormalities frequently occur in immature cattle with a poor prognosis, and the presence of CD205-positive cortical thymic epithelial cells is associated with the severity of the abnormalities.

    DOI: 10.1016/j.vetimm.2021.110379

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    PubMed

  • Reproductive cycle and maturation of Swinhoe's tree lizard (Diploderma swinhonis (Günther, 1864)) in Hyuga City, Miyazaki Prefecture, Japan

    Imatake S., Imaizumi N., Ohashi Y., Matsumura H., Urakawa M., Konaka Y., Kida T., Yanagita T., Fujisaki H., Wakitani S., Yasuda M.

    The Journal of veterinary medical science   82 ( 10 )   1551 - 1557   2020年10月

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:The Journal of veterinary medical science  

    Swinhoe's tree lizard (Diploderma swinhonis) is an arboreal agamid that is native to Taiwan. The species has been introduced to some areas of Japan and is regarded as an invasive alien species. In 2016, a nonnative population of D. swinhonis was discovered in Hyuga City, Miyazaki Prefecture, Japan, but little information was available on the ecology of the population at the time. The main purpose of this study was therefore to investigate the reproductive cycle and maturation of this population. Field research was conducted from 2017 to 2019, and 764 lizards were collected. Euthanized lizards were dissected and the reproductive organs were examined to determine the reproductive period, clutch size, clutch frequency and size at sexual maturity. Females with oviductal eggs or vitellogenic ovarian follicles were observed from May to October. Clutch size ranged from 2 to 8, and clutch frequency was more than twice a year. In males, spermiogenesis started in early May and testicular regression was observed in September. Males with spermatozoa in the epididymides were found from May to November. Minimum snout-vent length at sexual maturity was 50.2 mm in females and 53.0 mm in males. Comparisons of the findings of this study and reports from Taiwan suggest that the nonnative population of D. swinhonis in Hyuga City has a higher fecundity than populations in Taiwan. It is therefore considered necessary to exterminate the population in Hyuga City before this species colonizes other areas.

    DOI: 10.1292/jvms.20-0283

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    PubMed

  • Evidence that activin A directly modulates early human male germline differentiation status

    Moody S.C., Wakitani S., Young J.C., Western P.S., Loveland K.L.

    Reproduction (Cambridge, England)   160 ( 1 )   141 - 154   2020年7月

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Reproduction (Cambridge, England)  

    Disrupted fetal germline development underpins testicular germ cell neoplasia, which is increasing worldwide. The complex signaling milieu during normal testis development includes TGFβ superfamily ligands; this study tests the hypothesis that, activin A, a TGFβ superfamily member, can influence gonocyte development. The human seminoma-derived cell line, TCam-2, a model of fetal gonocytes, was cultured with activin A (1.25-25 ng/mL) for 48 h, or with 5 ng/mL activin A for short- (6, 24, and 48 h) and long-term (13 days) exposures, and downstream targets measured by qRT-PCR. Transcripts that exhibited significant dose-dependent responses to activin A included the early germ cell markers KIT, NODAL, and CRIPTO (NODALl co-receptor and activin inhibitor) which all increased and the differentiation marker DNMT3L which decreased. After 48 h, KIT, NODAL, and CRIPTO levels were significantly higher, while the differentiation marker NANOS2 was significantly lower. Interestingly, activin A exposure also significantly reduced both transcript and protein levels of the PIWI/piRNA pathway component DNMT3L. Because TCam-2 cells produce the activin inhibitor CRIPTO, CRIPTO was reduced using siRNA prior to activin A exposure. This selectively increased KIT in response to activin A. Other ligands present in the fetal testis (BMP4, FGF9, TGFβ1, and TGFβ2) induced distinct effects on germline marker expression. This study showed that activin A can directly modulate germline markers in this human gonocyte-like cell, promoting a less-differentiated phenotype. Additional findings indicate evidence of signaling crosstalk between activin A and NODAL, leading to target-specific effects on gonocyte differentiation.

    DOI: 10.1530/REP-20-0095

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    PubMed

  • The differentially DNA-methylated region responsible for expression of runt-related transcription factor 2

    Wakitani S., Wakitani S., Yokoi D., Hidaka Y., Nishino K.

    Journal of Veterinary Medical Science   79 ( 2 )   230 - 237   2017年2月

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Journal of Veterinary Medical Science  

    © 2017 The Japanese Society of Veterinary Science.Runt-related transcription factor 2 (Runx2) is essential for osteogenesis. This study aimes at identification of the genomic region differentially methylated in DNA for regulation of Runx2 expression. In the proximal promoter of mouse Runx2, DNA methylation was frequent at the region further than 3 kb relative to the transcription start site, in contrast to lower methylation status of the closer locus within 2 kb from the transcription start site. At the intermediate part, we identified a novel differentially methylated region in the Runx2 promoter region (Runx2-DMR): from -2.7 to -2.2 kb relative to the start site of Runx2 transcription in mice. In this region, the DNA methylation rate correlated negatively with Runx2 expression among mouse organs as well as among primary cultures of bone marrow from different dogs. Induction of mouse and dog mesenchymal-like cells into osteoblastic differentiation decreased the methylation rate of Runx2- DMR. Thus, in this study, we identified a novel genomic region in which DNA methylation status is related to Runx2 expression and detected demethylation of Runx2-DMR during osteoblastic differentiation in mouse and dog.

    DOI: 10.1292/jvms.16-0321

    Scopus

    PubMed

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書籍等出版物 【 表示 / 非表示

  • 獣医組織学 第8版

    日本獣医解剖学会( 担当: 分担執筆 ,  範囲: 第14章 心臓血管系)

    学窓社  2020年3月 

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    担当ページ:141-153   記述言語:日本語 著書種別:教科書・概説・概論

  • 平成31年度・令和元年度畜産生産に関する研究調査成果報告書

    脇谷 晶一, 保田 昌宏( 担当: 共著)

    一般財団法人 畜産ニューテック協会  2020年 

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    記述言語:日本語 著書種別:調査報告書

  • 獣医発生学 第2版

    T. A. McGeady, P. J. Quinn, E. S. Fitzpatrick, M. T. Ryan, D. Kilroy, P. Lonergan( 担当: 共訳 ,  範囲: 第14章 心臓血管系)

    学窓社  2019年2月 

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    担当ページ:141-153   記述言語:日本語 著書種別:教科書・概説・概論

  • 平成29年度食肉に関する助成研究調査成果報告書

    脇谷 晶一, 保田 昌宏( 担当: 共著)

    公益財団法人 伊藤記念財団  2018年11月 

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    記述言語:日本語 著書種別:調査報告書

MISC 【 表示 / 非表示

  • TGF-β superfamily signaling in testis formation and early male germline development

    Julia C. Young, Shoichi Wakitani, Kate L. Loveland

    Seminars in Cell & Developmental Biology   2015年10月

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    記述言語:英語   掲載種別:記事・総説・解説・論説等(学術雑誌)   出版者・発行元:Elsevier Ltd.  

講演・口頭発表等 【 表示 / 非表示

  • オキナワキノボリトカゲ背部皮膚における黒色素胞の分布

    岡 亮太朗、脇谷晶一、今竹翔一朗、保田昌宏

    九州両生爬虫類研究会 第12回大会 

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    開催年月日: 2022年2月19日

    会議種別:口頭発表(一般)  

  • スウィンホーキノボリトカゲ幼体の生殖器の形態的特徴

    今竹翔一朗、岡 亮太朗、脇谷晶一、保田昌宏

    九州両生爬虫類研究会 第12回大会 

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    開催年月日: 2022年2月19日

    会議種別:口頭発表(一般)  

  • 若齢黒毛和種廃用牛群の胸腺における細胞増殖と胸腺上皮細胞の分布

    川端 理紗子, 脇谷 晶一, 保田 昌宏

    第164回日本獣医学会学術集会 

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    開催年月日: 2021年9月7日 - 2021年9月13日

    記述言語:日本語   会議種別:口頭発表(一般)  

  • 若齢黒毛和種廃用牛群の胸腺における組織構造と遺伝子発現の多様性

    川端 理紗子, 脇谷 晶一, 久保田 綾音, 保田 昌宏

    第163回日本獣医学会学術集会 

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    開催年月日: 2020年9月14日 - 2020年9月30日

    記述言語:日本語   会議種別:口頭発表(一般)  

  • ウシ胸腺断片の器官培養中に起こる組織変化

    湖中雄悟, 脇谷晶一, 保田昌宏

    第162回日本獣医学会学術集会 

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    開催年月日: 2019年9月10日 - 2019年9月12日

    記述言語:日本語   会議種別:口頭発表(一般)  

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科研費(文科省・学振・厚労省)獲得実績 【 表示 / 非表示

  • ヒトiPS細胞のFGF依存性機構の解明:Nodalシグナリングの多重制御

    2016年04月 - 2018年03月

    科学研究費補助金  若手研究(B)

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    担当区分:研究代表者 

    ヒトiPS細胞のFGF依存性機構の解明:Nodalシグナリングの多重制御