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農学部 獣医学部門獣医学領域 |
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准教授 |
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論文 【 表示 ／ 非表示 】
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Wakitani S., Kawabata R., Shirataki S., Yasuda M.
Journal of Veterinary Medical Science 87 ( 2 ) 131 - 134 2025年
担当区分：筆頭著者, 責任著者 記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Journal of Veterinary Medical Science
Immunohistochemistry for keratins 5, 8, 14, and 18 was performed on Japanese Black calf thymuses at various stages of acute thymic involution. Keratins 5 and 14 were predominantly localized in the thymic medulla, while keratins 8 and 18 were broadly distributed throughout the parenchyma. Despite thymic involution, the distribution patterns of these keratins remained consistent. The cortical area, assessed by keratin 5 staining, progressively decreased with involution but retained approximately 40% of the total parenchyma even at the most severe stage. These results suggest that the thymic cortex shrinks but does not completely disappear during acute thymic involution in calves.
DOI： 10.1292/jvms.24-0413
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Furukawa R., Wakitani S., Kawabata R., Yasuda M.
Veterinary Journal 307 106225 2024年10月
担当区分：責任著者 記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Veterinary Journal
Transcriptome analysis was performed on the thymus of Japanese Black calves that were necropsied due to poor prognosis, to characterize changes associated with acute thymic involution. Gene expression profiles obtained by DNA microarray analysis of eight calf thymuses were classified into three patterns that correlated with the histopathological stage of acute thymic involution. Using principal component analysis, the first principal component of the global gene expression levels in the calf thymus was associated with the stage of acute thymic involution, suggesting that histopathological changes greatly influence the gene expression profile. Gene ontology enrichment analysis revealed that genes related to cell proliferation, wound healing, and inflammatory responses were the main contributors to the first principal component. Real-time RT-PCR showed that the thymus had lower expression of PCNA, KIFC1, and HES6, and higher expression of SYNPO2, PDGFRB, and TWIST1 during acute thymic involution. Immunohistochemistry demonstrated a decrease in the rate of Ki67-positive cells in the thymic cortex during the late stage of acute thymic involution. The rate of cleaved caspase-1-positive cells increased in the thymic cortex at an earlier stage than the increase in the rate of cleaved caspase-3-positive cells. Vimentin, which was almost absent in the non-involuted thymic cortex, appeared in the thymic cortex during acute thymic involution. These results suggest that in farmed calves with a poor prognosis, inflammatory responses and impaired thymocyte proliferation are primarily involved in acute thymic involution.
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Anatomical View of the Internal Carotid Artery Occlusion in Japanese Black Cattle 査読あり
Jadi A.R., Fujisaki H., Ramah A., Baakhtari M., Imatake S., Wakitani S., Yasuda M.
Animals 14 ( 3 ) 2024年2月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Animals
The internal carotid artery (ICA) is a branch of the common carotid artery (CCA), along with the external carotid artery (ECA), which together provide the blood supply for the brain. The description of the ICA in cattle is vague, including denial of its existence or degeneration at an early stage after birth. This anatomical study investigated the internal carotid artery in Japanese black cattle. Sixty-five heads of Japanese black cattle aged from newborn to 13 years were dissected and injected with colored latex from the CCA after separating the head and body. Diameter measurements of the artery branches from the CCA on its bifurcation were conducted. Furthermore, a histological examination of the ICA wall’s structures, which consist of the tunica intima, tunica media, and tunica externa, was performed. The ICA of Japanese black cattle is closed on the left side after age 3 years, except for a small lumen at 13 years, whereas the right ICA remains open at all ages. The location of occlusion of the left internal carotid artery (LICA) shows thickness of the tunica intima and an increased connective tissue layer area. The diameter of the ICA does not differ between the left and right sides, and there is no correlation with age. Therefore, further studies are needed, especially of ICA occlusion related to Japanese black cattle’s physiology or cerebrospinal disease.
DOI： 10.3390/ani14030365
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Nakanishi T., Suga K., Wakitani S., Yamaguchi K., Sugamoto K., Erickson L., Kawahara S.
European Journal of Lipid Science and Technology 126 ( 4 ) 2024年
掲載種別：研究論文（学術雑誌） 出版者・発行元：European Journal of Lipid Science and Technology
Phytanic acid is one of the branched-chain fatty acids in the human diet. Humans incorporate it into their bodies through the ingestion of ruminant meat and milk since these animal products contain the 3RS, 7R, 11R-isomer, as naturally occurring phytanic acid (nPA). Although phytanic acid has been reported to have health-promoting effects such as immunomodulatory effects, these findings were obtained mostly from in vitro cell-based experiments. In this study, the in vivo anti-inflammatory effects of nPA were investigated using a mouse model of dextran sulfate sodium (DSS)-induced colitis. Mice were fed diets containing 0%, 0.05%, or 0.1% nPA for 4 weeks. During the fourth week, acute colitis was induced by administration of a 3% DSS solution. The intake of 0.05% and 0.1% nPA inhibited DSS-induced body weight loss in mice, and showed improvement of histopathological changes in colon epithelium. nPA also inhibited the production of proinflammatory cytokines, especially macrophage-related cytokines such as interleukin-1β in the colon. These results suggest that nPA exerts anti-inflammatory effects under in vivo physiological conditions. Practical application: There is a growing demand for fatty acids possessing distinct nutritional and physical characteristics in the realms of health, nutrition, and food-related applications. The present study demonstrated that a branched-chain fatty acid, phytanic acid, exerts anti-inflammatory activity under not only in vitro but also in vivo conditions, using a commonly used mouse model of human inflammatory bowel disease. Our findings encourage further studies to determine whether phytanic acid elicits beneficial properties in humans, and suggest that phytanic acid enrichment can be a novel product strategy in the food market.
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Use of platelet lysate for in-vitro embryo production and treatment of repeat breeding in cows 査読あり
Hassaneen A.S.A., Rawy M.S., Yamanokuchi E., Elgendy O., Kawano T., Wakitani S., Kitahara G., Osawa T.
Theriogenology 210 199 - 206 2023年10月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Theriogenology
Platelet-rich plasma (PRP) is a biological hemocomponent derived from blood after the complete removal of red blood cells and the partial or complete removal of white blood cells to concentrate platelets in an appropriate volume of plasma. Platelets have important growth factors, cytokines, and active metabolites that improve the endometrial environment and positively affect implantation. This study evaluated the effect of the addition of activated PRP (platelets lysate; PL) on in vitro bovine oocyte maturation and embryonic development and the effect of intrauterine (IU) infusion of autologous PL in repeat breeder (RB) cows. Experiment 1 examined the effects of allogeneic PL, fetal calf serum (FCS), mixed PL + FCS, or platelet-poor plasma (PPP) supplementations to in vitro maturation and development media on in vitro oocyte maturation and embryo development in good- and poor-quality oocytes of Japanese Black cows. Experiment 2 examined the IU infusion of autologous PL, 24 h post-insemination, in 21 RB Holstein-Friesian dairy cows. The cleavage rate of good-quality oocytes was higher in the PL group (85.93 ± 2.50%) than in the PPP group (67.16 ± 3.41%) (P < 0.05), while the cleavage rate of the poor-quality oocytes was higher in the PL alone (76.13 ± 4.04%) and mixed PL + FCS treated (73.59 ± 4.22%) groups than in the PPP group (54.64 ± 2.93%) (P < 0.05). The blastocyst rate of the good-quality oocytes was higher in the PL group (40.97 ± 3.03%) than in the FCS (27.97 ± 3.31%) and PPP (25.33 ± 2.15%) groups (P < 0.05). The blastocyst rate of poor-quality oocytes and the hatching rates of both good and poor-quality oocytes showed no significant differences among all groups. The conception rate in the autologous PL-treated group was 41.67% (5/12), while it was 11.11% (1/9) in the control group. The platelets' count in the pregnant PL-treated cows (n = 5; mean ± SEM, 1.07 ± 0.10 × 109/mL) was higher than in the non-pregnant ones (n = 7; 0.67 ± 0.10 × 109/mL) (P < 0.05). In conclusion, allogeneic PL was effective in stimulating the in vitro oocyte maturation and embryonic development in both good and poor-quality bovine oocytes, and post-insemination IU infusion of autologous PL derived from high platelets’ count-PRP would be recommended for the treatment of RB cows.
書籍等出版物 【 表示 ／ 非表示 】
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獣医組織学 第8版
日本獣医解剖学会（ 担当： 分担執筆 , 範囲: 第14章 心臓血管系）
学窓社 2020年3月
担当ページ：141-153 記述言語：日本語 著書種別：教科書・概説・概論
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平成31年度・令和元年度畜産生産に関する研究調査成果報告書
脇谷 晶一, 保田 昌宏（ 担当： 共著）
一般財団法人 畜産ニューテック協会 2020年
記述言語：日本語 著書種別：調査報告書
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獣医発生学 第2版
T. A. McGeady, P. J. Quinn, E. S. Fitzpatrick, M. T. Ryan, D. Kilroy, P. Lonergan（ 担当： 共訳 , 範囲: 第14章 心臓血管系）
学窓社 2019年2月
担当ページ：141-153 記述言語：日本語 著書種別：教科書・概説・概論
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平成29年度食肉に関する助成研究調査成果報告書
脇谷 晶一, 保田 昌宏（ 担当： 共著）
公益財団法人 伊藤記念財団 2018年11月
記述言語：日本語 著書種別：調査報告書
MISC 【 表示 ／ 非表示 】
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TGF-β superfamily signaling in testis formation and early male germline development
Julia C. Young, Shoichi Wakitani, Kate L. Loveland
Seminars in Cell & Developmental Biology 2015年10月
記述言語：英語 掲載種別：記事・総説・解説・論説等（学術雑誌） 出版者・発行元：Elsevier Ltd.
講演・口頭発表等 【 表示 ／ 非表示 】
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ウシIARS異常症モデルマウス胸腺におけるLPS誘導性アポトーシスの亢進
奥原 千紗貴、脇谷 晶一、渡邉 正輝、岡村 匡史、佐々木 宣哉、保田 昌宏
第167回日本獣医学会学術集会 2024年9月11日
開催年月日： 2024年9月10日 - 2024年9月13日
記述言語：日本語 会議種別：口頭発表（一般）
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スウィンホーキノボリトカゲの性決定様式および孵卵温度による性比の変化
今竹翔一朗，佐藤暢，脇谷晶一，保田昌宏
第29回日本野生動物医学会大会
開催年月日： 2023年9月22日 - 2023年9月24日
記述言語：日本語 会議種別：口頭発表（一般）
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黒毛和種廃用子牛の胸腺の病理組織学的異常度と胸腺トランスクリプトームの関連
古川 涼悟、脇谷 晶一、川端 理紗子、保田 昌宏
第166回日本獣医学会学術集会
開催年月日： 2023年9月5日 - 2023年9月8日
記述言語：日本語 会議種別：口頭発表（一般）
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野鳥における前翼膜靭帯の比較解剖
深沢 英恵、杉山 真言、進藤 順治、脇谷 晶一、吉岡 一機
第166回日本獣医学会学術集会
開催年月日： 2023年9月5日 - 2023年9月8日
記述言語：日本語 会議種別：口頭発表（一般）
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新規骨軟骨染色法(JFRL 染色)の医療機器生物学的安全性試験への応用
辻絵里香，鷲見嘉奈子，中村鉄平，近藤大輔，辻尾祐志，細谷実里奈，難波貴志， 脇谷晶一，市居 修，西野光一郎，昆 泰寛，三嶋 隆，吉安友二，勝田真一
第44回日本バイオマテリアル学会大会 2022年11月22日
開催年月日： 2022年11月21日 - 2022年11月22日
記述言語：日本語 会議種別：ポスター発表
科研費（文科省・学振・厚労省）獲得実績 【 表示 ／ 非表示 】
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虚弱子牛症候群の低免疫状態に関与する胸腺皮質上皮細胞の特性解析
研究課題/領域番号：22K05988 2022年04月 - 2025年03月
独立行政法人日本学術振興会 科学研究費基金 基盤研究(C)
担当区分：研究代表者
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ヒトiPS細胞のFGF依存性機構の解明：Nodalシグナリングの多重制御
研究課題/領域番号：16K21243 2016年04月 - 2018年03月
独立行政法人日本学術振興会 科学研究費補助金 若手研究(B)
担当区分：研究代表者
ヒトiPS細胞のFGF依存性機構の解明：Nodalシグナリングの多重制御