脇谷 晶一 (ワキタニ ショウイチ)

WAKITANI Shoichi

写真a

職名

講師

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    -
    2008年03月

    山口大学   農学部   卒業

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    2011年09月

    山口大学  連合獣医学研究科  博士課程  修了

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  • 山口大学 -  博士(獣医学)

 

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  • The differentially DNA-methylated region responsible for expression of runt-related transcription factor 2

    Wakitani S., Wakitani S., Yokoi D., Hidaka Y., Nishino K.

    Journal of Veterinary Medical Science   79 ( 2 ) 230 - 237   2017年02月

    共著

     概要を見る

    © 2017 The Japanese Society of Veterinary Science.Runt-related transcription factor 2 (Runx2) is essential for osteogenesis. This study aimes at identification of the genomic region differentially methylated in DNA for regulation of Runx2 expression. In the proximal promoter of mouse Runx2, DNA methylation was frequent at the region further than 3 kb relative to the transcription start site, in contrast to lower methylation status of the closer locus within 2 kb from the transcription start site. At the intermediate part, we identified a novel differentially methylated region in the Runx2 promoter region (Runx2-DMR): from -2.7 to -2.2 kb relative to the start site of Runx2 transcription in mice. In this region, the DNA methylation rate correlated negatively with Runx2 expression among mouse organs as well as among primary cultures of bone marrow from different dogs. Induction of mouse and dog mesenchymal-like cells into osteoblastic differentiation decreased the methylation rate of Runx2- DMR. Thus, in this study, we identified a novel genomic region in which DNA methylation status is related to Runx2 expression and detected demethylation of Runx2-DMR during osteoblastic differentiation in mouse and dog.

    DOI PubMed CiNii

  • Tetraploid embryonic stem cells maintain pluripotency and differentiation potency into three germ layers

    Imai H., Imai H., Kano K., Kano K., Kano K., Fujii W., Takasawa K., Wakitani S., Hiyama M., Nishino K., Kusakabe K., Kiso Y.

    PLoS ONE   10 ( 6 ) e0130585   2015年06月

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    © 2015 Imai et al.Polyploid amphibians and fishes occur naturally in nature, while polyploid mammals do not. For example, tetraploid mouse embryos normally develop into blastocysts, but exhibit abnormalities and die soon after implantation. Thus, polyploidization is thought to be harmful during early mammalian development. However, the mechanisms through which polyploidization disrupts development are still poorly understood. In this study, we aimed to elucidate how genome duplication affects early mammalian development. To this end, we established tetraploid embryonic stem cells (TESCs) produced from the inner cell masses of tetraploid blastocysts using electrofusion of two-cell embryos in mice and studied the developmental potential of TESCs. We demonstrated that TESCs possessed essential pluripotency and differentiation potency to form teratomas, which differentiated into the three germ layers, including diploid embryonic stem cells. TESCs also contributed to the inner cell masses in aggregated chimeric blastocysts, despite the observation that tetraploid embryos fail in normal development soon after implantation in mice. In TESCs, stability after several passages, colony morphology, and alkaline phosphatase activity were similar to those of diploid ESCs. TESCs also exhibited sufficient expression and localization of pluripotent markers and retained the normal epigenetic status of relevant reprogramming factors. TESCs proliferated at a slower rate than ESCs, indicating that the difference in genomic dosage was responsible for the different growth rates. Thus, our findings suggested that mouse ESCs maintained intrinsic pluripotency and differentiation potential despite tetraploidization, providing insights into our understanding of developmental elimination in polyploid mammals. Copyright:

    DOI PubMed

  • Influence of atopic dermatitis on reproduction and uterine natural killer cells

    Hayashi K., Kusakabe K., Sugimoto S., Wakitani S., Sugi S., Kuniyoshi N., Hiyama M., Takeshita A., Kano K., Kiso Y.

    Journal of Veterinary Medical Science   76 ( 6 ) 913 - 916   2014年

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    The causal relationship between severe allergic conditions and successful pregnancy remains unclear. We aimed to evaluate reproductive performance in an experimental mouse model of atopic disease (AD), and the appearance of uterine natural killer (uNK) cells that have crucial roles in placental formation was examined. In the NC/Nga pregnant mice with moderate skin allergic lesions and an 8.6-fold elevation of plasma IgE, significant differences were not detected in the reproductive indices of the number of normal fetuses, abortion rate and placental size. There were few uNK cells in the placenta of AD mice, and they showed a significant decrease regarding the immature subtype as compared with controls. These findings revealed that AD disturbs uNK cell differentiation and provides disadvantageous effects on placental formation, although it does not arrest the pregnancy process. It may be possible that specific immunological conditions behind AD operate favorably to recover the reproductive performance. © 2014 The Japanese Society of Veterinary Science.

    DOI

  • Multiple Acyl-CoA Dehydrogenation Deficiency (Glutaric Aciduria Type II) with a Novel Mutation of Electron Transfer Flavoprotein-Dehydrogenase in a Cat.

    Wakitani S, Torisu S, Yoshino T, Hattanda K, Yamato O, Tasaki R, Fujita H, Nishino K

    JIMD reports   13   43 - 51   2014年

    共著

    DOI PubMed

  • Expression and localization of NO synthase isoenzymes (iNOS and eNOS) in development of the rabbit placenta.

    Khan H, Kusakabe KT, Wakitani S, Hiyama M, Takeshita A, Kiso Y

    The Journal of reproduction and development   58 ( 2 ) 231 - 6   2012年

    共著

    PubMed

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  • 獣医発生学 第2版

    T. A. McGeady, P. J. Quinn, E. S. Fitzpatrick, M. T. Ryan, D. Kilroy, P. Lonergan (担当: 共訳 , 担当範囲: 第14章 心臓血管系 )

    学窓社  2019年02月 ISBN: 978-4-87362-763-2

  • 平成29年度食肉に関する助成研究調査成果報告書

    脇谷 晶一, 保田 昌宏 (担当: 共著 )

    公益財団法人 伊藤記念財団  2018年11月

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  • TGF-β superfamily signaling in testis formation and early male germline development

    Julia C. Young, Shoichi Wakitani, Kate L. Loveland

    Seminars in Cell & Developmental Biology ( Elsevier Ltd. )    2015年10月

    総説・解説(学術雑誌)   共著

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  • ヒトiPS細胞のFGF依存性機構の解明:Nodalシグナリングの多重制御

    若手研究(B)

    研究期間:  2016年04月  -  2018年03月 

     概要を見る

    ヒトiPS細胞のFGF依存性機構の解明:Nodalシグナリングの多重制御

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  • ウシ胸腺断片の器官培養中に起こる組織変化

    湖中雄悟, 脇谷晶一, 保田昌宏

    第162回日本獣医学会学術集会  2019年09月  -  2019年09月   

  • 宮崎県日向市におけるスウィンホーキノボリトカゲの繁殖生態

    今竹翔一朗, 保田昌宏, 松村宙輝, 脇谷晶一, 岩本俊孝

    第162回日本獣医学会学術集会  2019年09月  -  2019年09月   

  • パントエアおよび乳酸菌の経口投与による子豚への免疫賦活作用

    浦川 真李夢, 保田 昌宏, 弓戸 猛, 大橋 勇希, Mahmoud Baakhtari, 脇谷 晶一, 志賀 明, 野津手 麻貴子, 阪上 健人

    第1 6 1 回日本獣医学会学術集会  2018年09月  -  2018年09月   

  • Effect of Branched Chain Amino Acid on immune status of young racing horses during training

    Mahmoud Baakhtari, 保田 昌宏, 浦川 真李夢, 弓戸 猛, 竹部 直矢, 都築 直, 岩本 洋平, 脇谷 晶一, 頃末 憲治

    第1 6 1 回日本獣医学会学術集会  2018年09月  -  2018年09月   

  • Primed型多能性幹細胞におけるNodal反応性遺伝子のFGF作用阻害によるアクチビン依存性発現変動

    脇谷晶一, 高澤建, 新井良和, 西野光一郎, 保田昌宏

    第17回日本再生医療学会総会  2018年03月  -  2018年03月   

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