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Faculty of Medicine College Hospital Department of Pathology |
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MORIGUCHI Sayaka
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Papers 【 display / non-display 】
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Maekawa Kazunari, Nakamura Eriko, Saito Yoichi, Matsuura Yunosuke, Gi Toshihiro, Nishihira Kensaku, Ooguri Nobuyuki, Moriguchi Sayaka, Sato Yuichiro, Hatakeyama Kinta, Shibata Yoshisato, Komohara Yoshihiro, Kaikita Koichi, Asada Yujiro, Yamashita Atsushi
PLoS ONE 20 ( 3 March ) e0316474 2025.3
Language：English Publishing type：Research paper (scientific journal)
Background
The thrombogenic potential of cells within atherosclerotic plaques is critical in the formation of a coronary thrombus. We hypothesized that a combination of inflammatory and hypoxic stimuli enhances tissue factor (TF) expression and glycolysis in cells in atherosclerotic plaques and contributes to coronary thrombus formation.
Aims
To identify TF- and hexokinase (HK)-II-expressing cells in coronary atherosclerotic plaques and thrombi and determine the effects of combined inflammatory and hypoxic stimuli and glycolysis on TF expression in peripheral blood mononuclear cell-derived macrophages.
Methods
We immunohistochemically assessed TF and HK-II expression in stable (n = 20) and unstable (n = 24) human coronary plaques and aspirated acute coronary thrombi (n = 15). The macrophages were stimulated with tumor necrosis factor-α, interferon-γ, or interleukin-10 under normoxic (21% O2) or hypoxic (1% O2) conditions, and TF expression was assessed.
Results
TF and HK-II expression were increased in unstable plaques compared with stable plaques. The number of CD68- and HK-II-immunopositive cells positively correlated with the number of TF-immunopositive cells. TF- and HK-II-expressing macrophages, which expressed M1- or M2-like markers, were involved in platelet-fibrin thrombus formation in ruptured plaques. The combination of inflammatory and hypoxic conditions additively augmented TF expression and procoagulant activity in the cultured macrophages. Inhibition of glycolysis with 2-deoxyglucose reduced the augmented TF expression and procoagulant activity.
Conclusion
Combined inflammatory and hypoxic conditions in atherosclerotic plaques may markedly enhance procoagulant activity in macrophages and contribute to coronary thrombus formation following plaque disruption. Macrophage TF expression may be associated with glycolysis. -
Expression of fibroblast activation protein-α in human deep vein thrombosis Reviewed
Oguri Nobuyuki, Gi Toshihiro, Nakamura Eriko, Furukoji Eiji, Goto Hiroki, Maekawa Kazunari, Tsuji Atsushi B., Nishii Ryuichi, Aman Murasaki, Moriguchi Sayaka, Sakae Tatefumi, Azuma Minako, Yamashita Atsushi
Thrombosis Research 241 109075 2024.9
Language：English Publishing type：Research paper (scientific journal)
Background
Fibroblast activation protein-α (FAP), a type-II transmembrane serine protease, is associated with wound healing, cancer-associated fibroblasts, and chronic fibrosing diseases. However, its expression in deep vein thrombosis (DVT) remains unclear. Therefore, this study investigated FAP expression and localization in DVT.
Methods
We performed pathological analyses of the aspirated thrombi of patients with DVT (n = 14), classifying thrombotic areas in terms of fresh, cellular lysis, and organizing reaction components. The organizing reaction included endothelialization and fibroblastic reaction. We immunohistochemically examined FAP-expressed areas and cells, and finally analyzed FAP expression in cultured dermal fibroblasts.
Results
All the aspirated thrombi showed a heterogeneous mixture of at least two of the three thrombotic areas. Specifically, 83 % of aspirated thrombi showed fresh and organizing reaction components. Immunohistochemical expression of FAP was restricted to the organizing area. Double immunofluorescence staining showed that FAP in the thrombi was mainly expressed in vimentin-positive or α-smooth muscle actin-positive fibroblasts. Some CD163-positive macrophages expressed FAP. FAP mRNA and protein levels were higher in fibroblasts with low-proliferative activity cultured under 0.1 % fetal bovine serum (FBS) than that under 10 % FBS. Fibroblasts cultured in 10 % FBS showed a significant decrease in FAP mRNA levels following supplementation with hemin, but not with thrombin.
Conclusions
The heterogeneous composition of venous thrombi suggests a multistep thrombus formation process in human DVT. Further, fibroblasts or myofibroblasts may express FAP during the organizing process. FAP expression may be higher in fibroblasts with low proliferative activity. -
Otani T., Moriguchi-Goto S., Nishihira K., Oguri N., Shibata Y., Matsuura Y., Kodama T., Asada Y., Hatakeyama K., Yamashita A.
Thrombosis Research 234 134 - 141 2024.2
Publishing type：Research paper (scientific journal) Publisher：Thrombosis Research
Aim: To investigate the role of pentraxin 3 (PTX3) in atherosclerotic disease progression and plaque destabilization, as well as in coronary restenosis after directional coronary atherectomy (DCA). Materials and methods: PTX3 contents of early and advanced atherosclerotic lesions of the aorta obtained at autopsy were determined by ELISA and Western blot. Also, coronary plaques of patients with acute coronary syndrome (ACS) or stable angina pectoris (SAP) obtained by DCA were analyzed by immunohistochemistry for PTX3. The effects of PTX3 on smooth muscle cells (SMCs) and thrombogenesis were investigated with cultured human coronary artery SMCs and a flow chamber system, respectively. Results: Advanced atherosclerotic lesions contained a significantly larger amount of PTX3 than early lesions (ELISA: 9.96 ± 2.77 ng/100 mg tissue, n = 8 vs 0.24 ± 0.18 ng/100 mg tissue, n = 6, P = 0.0097). Also, ACS plaques contained a significantly larger amount of PTX3 than SAP plaques (PTX3 immunohistochemistry–positive area percentage: 2.88 ± 0.53 %, n = 22 vs 0.67 ± 0.27 %, n = 23, P = 0.0009). Curiously, the patients who would remain free of post-DCA restenosis (n = 19) had plaques with a significantly higher PTX3 immunohistochemistry–positive area percentage than those who would develop restenosis (n = 12) (2.32 ± 0.49 % vs 0.49 ± 0.17 %, P = 0.002). In the mechanistic part of the study, PTX3 inhibited SMC proliferation and migration. PTX3 also inhibited platelet thrombus formation in the condition simulating arterial blood flow. Conclusions: PTX3 is increased in advanced (vs early) atherosclerotic lesions and unstable (vs stable) coronary plaques. The inhibitory effects of PTX3 on SMCs and thrombogenesis suggest that intraplaque PTX3 might have atheroprotective effects.
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Gi T., Kuwahara A., Yamashita A., Matsuda S., Maekawa K., Moriguchi-Goto S., Sato Y., Asada Y.
Arteriosclerosis, Thrombosis, and Vascular Biology 43 ( 1 ) 146 - 159 2023.1
Language：English Publishing type：Research paper (scientific journal) Publisher：Arteriosclerosis, Thrombosis, and Vascular Biology
Background: Cancer-associated venous thromboembolism (VTE) is a critical complication in patients with cancer. However, the pathological findings of VTE are limited. Here, we investigated the histopathological features of cancer-associated VTE in human autopsy cases. Methods: We clinically examined the autopsy cases of VTE with (n=114) and without cancer (n=66) and immunohistochemically analyzed the expression of prothrombotic factors in intrathrombus cancer cells, the thrombus contents of erythrocytes, fibrin, platelets, citrullinated histone H3, and degree of organization. Results: Vascular wall invasion or small cell clusters of cancer cells was observed in thrombi in 27.5% of deep vein thrombosis and 25.9% of pulmonary embolism cases. The majority of the cancer cells in deep vein thrombi appeared to be invading the vessel wall, whereas the majority of pulmonary thrombi had cancer cell clusters, consistent with embolization via blood flow. These cancer cells were immunohistochemically positive for TF (tissue factors) or podoplanin in up to 88% of VTE cases. The frequency of TF-positive monocyte/macrophages in thrombi was higher in cancer-associated VTE than that in VTE without cancer. Citrullinated histone H3 was predominantly observed in the early stages of organizing thrombi. There was no significant difference in thrombus components between VTE with cancer and without cancer groups. Conclusions: Vascular wall invasion or cancer cell clusters in thrombi might influence thrombogenesis of cancer-associated VTE. TF and podoplanin in cancer cells and in monocyte/macrophages may induce coagulation reactions and platelet aggregation. Neutrophil extracellular traps may play a role in the early stages of VTE, regardless of cancer status.
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Usefulness of the Sydney system for lymph node imprint smear cytology Reviewed
MEIRI Misato, NOGUCHI Hiroshi, MORIGUCHI Sayaka, SATO Yuichiro
The Journal of the Japanese Society of Clinical Cytology 64 ( 3 ) 121 - 129 2025.5
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：The Japanese Society of Clinical Cytology
<i><b>Objective</b></i> : The Sydney system was recently proposed for classifying and reporting the findings of fine-needle aspiration cytology in lymphadenopathy. We investigated the usefulness of the Sydney system in reporting the findings of imprint smear cytology.<i><b>Study Design</b></i> : A total of 118 samples were re-evaluated and classified according to the Sydney system. At the first diagnostic level, we classified the samples into five categories (inadequate/insufficient, benign, atypical cells/atypical of lymphoid cells, suspicious, and malignant), based only on the cytomorphological findings. Samples were then reclassified using ancillary techniques at the second level. The percentages in each category at the two levels were calculated and compared between the two diagnostic levels. In addition, we sub-classified the lymphoma samples and evaluated the diagnostic agreement rates for each level.<i><b>Results</b></i> : At the first diagnostic level, 57.6％ of the samples were classified as malignant. At the second diagnostic level, 85.1％ of the samples were classified as malignant. The percentage of samples diagnosed as malignant was significantly higher at the second level than at the first level (p＜0.01). The diagnostic agreement was 74.6％ for the first level and 83.6％ for the second level.<i><b>Conclusions</b></i> : Imprint smear cytology is suitable for lymphoma diagnosis under the guidance of the Sydney system, and the diagnostic accuracy is high.
DOI： 10.5795/jjscc.64.121
MISC 【 display / non-display 】
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An autopsy case of elderly-onset acute necrotizing encephalopathy secondary to influenza.
Ishii N, Mochizuki H, Moriguchi-Goto S, Shintaku M, Asada Y, Taniguchi A, Shiomi K, Nakazato M.
Journal of neurological sciences 2015.7
Language：English Publishing type：Article, review, commentary, editorial, etc. (scientific journal) Publisher：Elsevier.
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病理学の立場からみたDICの病態
盛口清香、丸塚浩助
血栓と循環 2015.6
Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media) Publisher：メディカルレビュー社
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A case of myxoid smooth muscle tumor of the uterus.
Sayaka Moriguchi-Goto, Kazuko Fukushima, Hiroshi Samesima, Yujiro Asada, Yuichiro Sato
5 ( 2 ) 61 - 63 2014.5
Language：Japanese Publishing type：Research paper, summary (national, other academic conference)
Presentations 【 display / non-display 】
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A case of trisomy 8-positive myelodysplastic syndrome with intestinal Behcet-like disease
Event date： 2023.11.9 - 2023.11.10
Language：Japanese Presentation type：Poster presentation
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真性癒着胎盤の臨床病理組織学的検討
佐藤勇一郎、盛口清香、山下 篤、浅田 祐士郎、鮫島 浩
第24回日本胎盤学会学術集会
Event date： 2016.11.25 - 2016.11.26
Language：Japanese Presentation type：Poster presentation
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神経内分泌細胞癌成分を伴った胃腺癌の1切除例
中尾大伸、河野文彰、田代耕盛、森浩貴、緒方祥吾、伊藤歌織、市成直樹、武野慎祐、池田拓人、七島篤志、中村都英、盛口淸香、佐藤勇一郎
第78回日本臨床外科学会総会
Event date： 2016.11.24 - 2016.11.26
Language：Japanese Presentation type：Poster presentation
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Atypical teratoid/rhabdoid tumorの2例
徳満貴子、野口裕史、大野招伸、峰松映子、森田勝代、黒木栄輝、福島剛、盛口清香、佐藤勇一郎
第55回日本臨床細胞学会
Event date： 2016.11.18 - 2016.11.19
Language：Japanese Presentation type：Poster presentation
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眼付属器に発生した低異型度B細胞性リンパ腫の4症例
野口裕史、徳満貴子、大野招伸、峰松映子、森田勝代、黒木栄輝、盛口清香、佐藤勇一郎
第56回日本臨床細胞学会
Event date： 2016.11.18 - 2016.11.19
Language：Japanese Presentation type：Poster presentation
Grant-in-Aid for Scientific Research 【 display / non-display 】
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重症熱性血小板減少症候群における血小板減少と血液凝固異常の病態解明
Grant number：23K06425 2023.04 - 2026.03
独立行政法人日本学術振興会 科学研究費基金 基盤研究(C)
Authorship：Principal investigator