論文 - 関口 敏
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Wu X., Notsu K., Matsuura Y., Mitoma S., El Daous H., Norimine J., Sekiguchi S.
Journal of Virological Methods 315 114706 2023年5月
担当区分：最終著者, 責任著者 記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Journal of Virological Methods
Bovine leukemia virus (BLV) is the causative agent of a B-cell tumor called enzootic bovine leukosis. Preventing BLV spreading is required to reduce economic loss related to BLV infection of livestock. To quantify proviral load (PVL) more easily and rapidly, we developed a quantification system of PVL using droplet digital PCR (ddPCR). This method uses a multiplex TaqMan assay of the BLV provirus and housekeeping gene RPP30 for the quantification of BLV in BLV-infected cells. Furthermore, we combined ddPCR with DNA purification-free sample preparation (unpurified genomic DNA). The percentage of BLV-infected cells based on unpurified genomic DNA was highly correlated with that based on purified genomic DNA (correlation coefficient: 0.906). Thus, this new technique is a suitable method to quantify PVL of BLV-infected cattle in a large sample number.
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Identifying Pathogen and Allele Type Simultaneously in a Single Well Using Droplet Digital PCR. 査読あり 国際共著
Notsu K, El Daous H, Mitoma S, Wu X, Norimine J, Sekiguchi S
mSphere 8 ( 1 ) e0049322 2023年1月
担当区分：最終著者, 責任著者 記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：mSphere
In the transmission control of chronic and untreatable livestock diseases such as bovine leukemia virus (BLV) infection, the removal of viral superspreaders is a fundamental approach. On the other hand, selective breeding of cattle with BLV-resistant capacity is also critical for reducing the viral damage to productivity by keeping infected cattle. To provide a way of measuring BLV proviral load (PVL) and identifying susceptible/resistant cattle simply and rapidly, we developed a fourplex droplet digital PCR method targeting the BLV pol gene, BLV-susceptible bovine major histocompatibility complex (BoLA)-DRB3*016:01 allele, resistant DRB3*009:02 allele, and housekeeping RPP30 gene (IPATS-BLV). IPATS-BLV successfully measured the percentage of BLV-infected cells and determined allele types precisely. Furthermore, it discriminated homozygous from heterozygous carriers. Using this method to determine the impact of carrying these alleles on the BLV PVL, we found DRB3*009:02-carrying cattle could suppress the PVL to a low or undetectable level, even with the presence of a susceptible heterozygous allele. Although the population of DRB3*016:01-carrying cattle showed significantly higher PVLs compared with cattle carrying other alleles, their individual PVLs were highly variable. Because of the simplicity and speed of this single-well assay, our method has the potential of being a suitable platform for the combined diagnosis of pathogen level and host biomarkers in other infectious diseases satisfying the two following characteristics of disease outcomes: (i) pathogen level acts as a critical maker of disease progression; and (ii) impactful disease-related host genetic biomarkers are already identified. IMPORTANCE While pathogen-level quantification is an important diagnostic of disease severity and transmissibility, disease-related host biomarkers are also useful in predicting outcomes in infectious diseases. In this study, we demonstrate that combined proviral load (PVL) and host biomarker diagnostics can be used to detect bovine leukemia virus (BLV) infection, which has a negative economic impact on the cattle industry. We developed a fourplex droplet digital PCR assay for PVL of BLV and susceptible and resistant host genes named IPATS-BLV. IPATS-BLV has inherent merits in measuring PVL and identifying susceptible and resistant cattle with superior simplicity and speed because of a single-well assay. Our new laboratory technique contributes to strengthening risk-based herd management used to control within-herd BLV transmission. Furthermore, this assay design potentially improves the diagnostics of other infectious diseases by combining the pathogen level and disease-related host genetic biomarker to predict disease outcomes.
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Notsu K, Daous HE, Mitoma S, Norimine J, Sekiguchi S
HLA 99 ( 1 ) 12 - 24 2021年11月
担当区分：最終著者, 責任著者 記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：HLA
As genetically resistant individuals, the “elite controllers” (ECs) of human immunodeficiency virus infection have been focused on as the keys to developing further functional treatments in medicine. In the livestock production field, identifying the ECs of bovine leukemia virus (BLV) infection in cattle is desired to stop BLV transmission chains on farms. Cattle carrying the bovine leukocyte antigen (BoLA)-DRB3*009:02 allele (DRB3*009:02) have a strong possibility of being BLV ECs. Most of cattle carrying this allele maintain undetectable BLV proviral loads and do not shed virus even when infected. BLV ECs can act as transmission barriers when placed between uninfected and infected cattle in a barn. To identify cattle carrying DRB3*009:02 in large populations more easily, we developed a pooled testing system. It employs a highly sensitive, specific real-time PCR assay and TaqMan MGB probes (DRB3*009:02-TaqMan assay). Using this system, we determined the percentage of DRB3*009:02-carrying cattle on Kyushu Island, Japan. Our pooled testing system detected cattle carrying the DRB3*009:02 allele from a DNA pool containing one DRB3*009:02-positive animal and 29 cattle with other alleles. Its capacity is sufficient for herd-level screening for DRB3*009:02-carrying cattle. The DRB3*009:02-TaqMan assay showed high-discriminative sensitivity and specificity toward DRB3*009:02, making it suitable for identifying DRB3*009:02-carrying cattle in post-screening tests on individuals. We determined that the percentage of DRB3*009:02-carrying cattle in Kyushu Island was 10.56%. With its ease of use and reliable detection, this new method strengthens the laboratory typing for DRB3*009:02-carrying cattle. Thus, our findings support the use of BLV ECs in the field.
DOI： 10.1111/tan.14502
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Daous H.E., Mitoma S., Elhanafy E., Huyen N.T., Ngan M.T., Notsu K., Kaneko C., Norimine J., Sekiguchi S.
Animals 11 ( 3 ) 1 - 14 2021年3月
担当区分：最終著者, 責任著者 記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Animals
Enzootic bovine leukosis is a lethal neoplastic disease caused by bovine leukemia virus (BLV), belongs to family Retroviridae. The BLV proviral load (PVL) represents the quantity of BLV genome that has integrated into the host’s genome in BLV-infected cells. Bovine leukocyte antigen (BoLA) class II allelic polymorphisms are associated with PVLs in BLV-infected cattle. We sought to identify relationships between BoLA-DRB3 allelic heterozygosity and BLV PVLs among different cattle breeds. Blood samples from 598 BLV-infected cattle were quantified to determine their PVLs by real-time polymerase chain reaction. The results were confirmed by a BLV-enzyme-linked immunosorbent assay. Restriction fragment length polymorphism-polymerase chain reaction identified 22 BoLA-DRB3 alleles. Multivariate negative binomial regression modeling was used to test for associations between BLV PVLs and BoLA-DRB3 alleles. BoLA-DRB3.2*3, *7, *8, *11, *22, *24, and *28 alleles were significantly associated with low PVLs. BoLA-DRB3.2*10 was significantly associated with high PVLs. Some heterozygous allele combinations were associated with low PVLs (*3/*28, *7/*8, *8/*11, *10/*11, and *11/*16); others were associated with high PVLs (*1/*41, *10/*16, *10/*41, *16/*27, and *22/*27). Interestingly, the BoLA-DRB3.2*11 heterozygous allele was always strongly and independently associated with low PVLs. This is the first reported evidence of an association between heterozygous allelic combinations and BLV PVLs.
DOI： 10.3390/ani11030647
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Mitoma S., Carr B.V., Harvey Y., Moffat K., Sekiguchi S., Charleston B., Norimine J., Seago J.
Immunology 164 ( 2 ) 266 - 278 2021年
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Immunology
Foot-and-mouth disease (FMD) is a highly contagious, economically devastating disease of cloven-hooved animals. The development of long-lasting effective FMD vaccines would greatly benefit the global FMD control programme. Deep analysis of adaptive immunity in cattle vaccinated against FMD is technically challenging due to the lack of species-specific tools. In this study, we aimed to identify CD4+ T-cell epitopes in the FMD virus (FMDV) capsid and to phenotype the CD4+ T cells that recognize them using bovine major histocompatibility complex (BoLA) class II tetramer. A BoLA class II tetramer based on the DRA/DRB3*020:02 allele and FMDV antigen-stimulated PBMCs from bovine vaccinates were used to successfully identify four epitopes in the FMDV capsid, three of which have not been previously reported; two epitopes were identified in the structural protein VP1, one in VP3 and one in VP4. Specificity of the three novel epitopes was confirmed by proliferation assay. All epitope-expanded T-cell populations produced IFN-γ in vitro, indicating a long-lasting Th1 cell phenotype after FMD vaccination. VP3-specific CD4+ T cells exhibited the highest frequency amongst the identified epitopes, comprising >0·004% of the CD4+ T-cell population. CD45RO+CCR7+ defined central memory CD4+ T-cell subpopulations were present in higher frequency in FMDV-specific CD4+ T-cell populations from FMD-vaccinated cattle ex vivo. This indicates an important role in maintaining cell adaptive immunity after FMD vaccination. Notably, FMDV epitope-loaded tetramers detected the presence of FMDV-specific CD4+ T cells in bovine PBMC more than four years after vaccination. This work contributes to our understanding of vaccine efficacy.
DOI： 10.1111/imm.13367
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Notsu K., Wiratsudakul A., Mitoma S., Daous H.E., Kaneko C., El-Khaiat H.M., Norimine J., Sekiguchi S.
Pathogens 9 ( 11 ) 1 - 12 2020年11月
担当区分：責任著者 記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Pathogens
© 2020 by the authors. Licensee MDPI, Basel, Switzerland. The cattle industry is suffering economic losses caused by bovine leukemia virus (BLV) and enzootic bovine leukosis (EBL), the clinical condition associated with BLV infection. This pathogen spreads easily without detection by farmers and veterinarians due to the lack of obvious clinical signs. Cattle movement strongly contributes to the inter-farm transmission of BLV. This study quantified the farm-level risk of BLV introduction using a cattle movement analysis. A generalized linear mixed model predicting the proportion of BLV-infected cattle was constructed based on weighted in-degree centrality. Our results suggest a positive association between weighted in-degree centrality and the estimated number of introduced BLV-infected cattle. Remarkably, the introduction of approximately six cattle allowed at least one BLV-infected animal to be added to the farm in the worst-case scenario. These data suggest a high risk of BLV infection on farms with a high number of cattle being introduced. Our findings indicate the need to strengthen BLV control strategies, especially along the chain of cattle movement.
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Mai T.N., Bui T.P., Huynh T.M.L., Sasaki Y., Mitoma S., Daous H.E., Fahkrajang W., Norimine J., Sekiguchi S.
Frontiers in Veterinary Science 7 433 2020年7月
担当区分：責任著者 記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Frontiers in Veterinary Science
© Copyright © 2020 Mai, Bui, Huynh, Sasaki, Mitoma, Daous, Fahkrajang, Norimine and Sekiguchi. Porcine epidemic diarrhea virus (PEDV) causes enteritis, vomiting, watery diarrhea, and high mortality in suckling pigs, threatening the swine industry. Porcine epidemic diarrhea (PED) re-emerged globally in 2013 in many important swine-producing countries in Asia and the Americas. Several studies have identified the risk factors for the spread of PEDV in acute outbreaks. However, limited information is available on the risk factors for the transmission of PEDV in endemic regions. We hypothesized that poor biosecurity, location, and some social or cultural practices are the main risk factors for PEDV transmission in the Vietnamese pig population. The aim of this study was to evaluate the potential risk factors for the transmission of PEDV in an endemic area in Vietnam. In this case–control study, questionnaires containing 51 questions were completed for 92 PEDV-positive and 95 PEDV-negative farms. A logistic regression analysis was performed to assess the risk factors associated with PEDV infection. Province and the total number of pigs were included as random effects to determine their influence on the risk of PEDV infection. Twenty-nine variables of interest that have been associated with PEDV status were analyzed in a univariate analysis (P <0.20), with backward stepwise selection. Only three of these 29 variables in four models remained significant PEDV risk factors in the final model: farrow-to-wean production type, distance from the farm to the slaughterhouse (<1,000 m), and the presence of chickens on site (P <0.05). This is the first study to identify the main risk factors for PEDV infection in an endemic area. Our findings suggest that hygiene measures should be strictly implemented on farms for the effective control and prevention of PEDV infection.
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A questionnaire survey of the illegal importation of pork products by air travelers into Japan from China and exploration of causal factors. 査読あり 国際共著
Lei Z, Haga T, Obara H, Sekiyama H, Sekiguchi S, Hombu A, Fujihara M, Lei L, Hsu S, Zhang X, Ishitsuka I, Atagi Y, Sato T, Sugiura K
Preventive veterinary medicine 177 104947 2020年4月
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Establishment of a novel diagnostic test for Bovine leukaemia virus infection using direct filter PCR. 査読あり 国際共著
El Daous H, Mitoma S, Elhanafy E, Thi Nguyen H, Thi Mai N, Hara A, Duangtathip K, Takezaki Y, Kaneko C, Norimine J, Sekiguchi S
Transboundary and emerging diseases 2020年2月
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Investigating BoLA Class II DRB3*009:02 carrying cattle in Japan 査読あり
Fujimori S., Ando T., Sekiguchi S., Notsu K., Ishida S., Daidoji T., Hagiwara K.
Veterinary and Animal Science 27 100425 2025年3月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Veterinary and Animal Science
Enzootic bovine leukosis (EBL) is a malignant lymphoma of cattle that is mainly caused by bovine leukemia virus (BLV) infection. In this study, PCR-RFLP was used to investigate the frequency of the DRB3*009:02 allele in several farms with different herd management practices in Japan. A total of 742 Holsteins (384) and Japanese Blacks (230) were used as the sample size for the study, which was larger than the number of cattle in the study area with a confidence level of 95 % and a margin of error of 8. PBMCs isolated from whole blood from clinically healthy cattle were used for examination. The presence of BLV provirus infection was determined by qPCR targeting the env region. BLV antibodies were detected using a commercial ELISA kit. The results showed that 35 cattle were heterozygous for DRB3*009:02. The frequency on each farm varied between farms, and PCR analysis showed that the prevalence of BLV also varied between farms. The incidence rate (4.7 %) was lower than in previous studies. The BLV seroprevalence (14.4 %) in this study was lower than the BLV infection rate (35 %) in the study. Holstein dairy cows had low levels of BL resistance genes, confirming the spread of the virus within the farm's herd. Cattle on farms with low BLV-positive rates had a resistance gene-carrying rate of 16.9 %, meanwhile, those who did not carry the resistance gene had a rate of 91.4 %. BLV provirus levels vary between farmers, with herds carrying low BL-resistance genes tending to have higher levels of BLV provirus. In light of the current BLV epidemic, herd composition reform, along with aggressive breeding of BL-resistant sires, is a required component to increase the herd of BL-resistant sires and improve livestock productivity.
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Isoda N, Sekiguchi S, Ryu C, Notsu K, Kobayashi M, Hamaguchi K, Hiono T, Ushitani Y, Sakoda Y
Viruses 17 ( 1 ) 2025年1月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Viruses
Bovine viral diarrhea (BVD) is caused by the BVD virus (BVDV) and has been reported worldwide in cattle. To estimate BVDV circulation among cattle where few BVD cases were reported in southern Japan, 1910 serum samples collected from 35 cattle farms without a BVD outbreak were investigated to detect antibodies against BVDV-1 and BVDV-2 using an indicator virus with a cytopathogenic effect and the luciferase gene, respectively. Neutralizing antibodies against BVDV-1 and BVDV-2 were detected more frequently in 18 vaccinated farms than in 17 nonvaccinated farms. In the nonvaccinated farms, 9.6%, 1.8%, and 13.8% of the cattle were estimated to have a history of infection with BVDV-1, BVDV-2, and both, respectively. The median rate of within-herd anti-BVDV-1 seropositivity among cattle in the nonvaccinated farms was 22.0%; however, a high within-herd seropositivity (>50%) was confirmed in the two farms. The force of infection, basic reproduction number, and annual probability of BVDV-1 infection were estimated as 0.072 (95% confidence interval [CI]: 0.062–0.084), 0.36 (95% CI: 0.31–0.42), and 0.73% (95% CI: 0.61–0.87%), respectively, using the age-specific positive rate of anti-BVDV-1 antibodies. These parameters should be further applicable for developing epidemiological models which illustrate the BVDV dynamics in the field.
DOI： 10.3390/v17010061
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Direct TaqMan assay for the detection and genotyping of bovine viral diarrhea virus types 1 and 2 査読あり
Ullah S., Notsu K., Saito A., Okabayashi T., Mekata H., Isoda N., Sekiguchi S.
Archives of Virology 170 ( 1 ) 15 2025年1月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Archives of Virology
Bovine viral diarrhea (BVD), caused by bovine viral diarrhea virus (BVDV), has a significant economic impact on affected farms worldwide. For effective disease control, it is crucial to select an appropriate vaccine based on the specific genotype of BVDV. Therefore, developing a rapid and reliable assay to detect and genotype BVDV is imperative for controlling the spread of disease. In this study, we developed a TaqMan assay to detect and genotype BVDV types 1 and 2 directly in bovine serum without extraction of RNA. The direct BVDV TaqMan assay effectively detected both BVDV1 and BVDV2 with confirmed specificity and showed no cross-reactivity with any of the other viruses tested, including bovine respiratory syncytial virus, bovine coronavirus, Akabane virus, bovine herpesvirus 1, bovine parainfluenza virus 3, bovine immunodeficiency virus, and bovine leukemia virus. The assay could detect the virus in serum samples with a titer as low as 102 TCID50/mL in two out of three trials for BVDV1 and all three trials for BVDV2, indicating that its sensitivity is equivalent to that of virus isolation. Our findings represent a significant advancement in BVDV detection and typing directly from bovine serum.
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Wongnak P., Yano T., Sekiguchi S., Chalvet-Monfray K., Premashthira S., Thanapongtharm W., Wiratsudakul A.
Preventive Veterinary Medicine 230 106282 2024年9月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Preventive Veterinary Medicine
Foot-and-mouth disease (FMD) is an important endemic disease in livestock in Southeast Asia. Transboundary movement of animals may result in the transnational disease spread. A major cattle market is located at the Thailand-Myanmar border, where most cattle imported from Myanmar are traded. In this study, we built a stochastic susceptible-exposed-infectious-recovered (SEIR) model to investigate the effectiveness of a private animal quarantine service center in preventing FMDV from entering the major cattle market. We computed with different parameters and found that, with 50 % vaccine effectiveness, the risk of releasing infected cattle to the market per batch was generally low during the quarantine period of 21 and 28 days, with the risk ranging from 0.071 to 0.078 and 0.032 to 0.036, respectively. Despite the best scenario, the zero-risk state is difficult to attain. The sensitivity analysis highlights that the percentage of immune animals before entering the quarantine centers and the vaccine effectiveness are important factors. In conclusion, the 21-day quarantine period mitigates the risk of FMDV introduction into the cattle market. This control measure should be rigorously maintained to sustainably prevent FMDV outbreaks through transboundary animal movements, especially among countries in FMD-endemic regions.
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F<sub>1</sub>母豚およびバークシャー種母豚における産次・季節・群構成間の生産性の評価 査読あり
廣川 万莉, 関口 敏, 佐々木 羊介
獣医疫学雑誌 28 ( 1 ) 40 - 49 2024年7月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：獣医疫学会
本研究では，バークシャー種母豚およびランドレース種と大ヨークシャー種のF<sub>1</sub>母豚を対象として，産次および季節間の生産性を評価することを目的とした。本研究は，南九州において，F<sub>1</sub>母豚を飼養する1農場と，バークシャー種母豚を飼養する1農場を対象とした。本研究では2018-2020年における分娩記録および初回交配記録を分析に用いた。F<sub>1</sub>母豚の分娩率を除き，両品種において初産母豚の個体生産成績が最も低かった（P<0.05）。一方で，産子数のピークは，F<sub>1</sub>母豚では2-4産であったのに対し，バークシャー種では3-6産であった。F<sub>1</sub>母豚およびバークシャー種ともに夏季分娩において低い産子数および分娩率がみられた（P<0.05）。F<sub>1</sub>母豚を飼養する農場における週間生産性と群構成との関連性について，週当たりの母豚数が増加するにつれて週当たりの離乳後初回交配日数7日以内の母豚割合および週当たりの平均分娩率が減少した（P<0.05）。バークシャー種母豚を飼養する農場における週間生産性と群構成との関連性について，週当たりの初産母豚割合が増加するにつれて週当たりの離乳後初回交配日数7日以内の母豚割合および週当たりの平均分娩率が減少した（P<0.05）。F<sub>1</sub>母豚とバークシャー種で産次間の生産性の傾向が異なることが示唆されたため，飼養管理や廃用計画などは品種によって変えることが推奨される。
DOI： 10.2743/jve.28.40
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Narita T., Kubo M., Nagakura Y., Sekiguchi S.
Porcine Health Management 10 ( 1 ) 6 2024年1月
担当区分：最終著者, 責任著者 記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Porcine Health Management
Background: Data on abnormal health conditions in animals obtained from slaughter inspection are important for identifying problems in fattening management. However, methods to objectively evaluate diseases on farms using inspection data has not yet been well established. It is important to assess fattening management on farms using data obtained from slaughter inspection. In this study, we developed the state-space model to evaluate swine morbidity using slaughter inspection data. Results: The most appropriate model for each disease was constructed using the state-space model. Data on 11 diseases in slaughterhouses over the past 4 years were used to build the model. The model was validated using data from 14 farms. The local-level model (the simplest model) was the best model for all diseases. We found that the analysis of slaughter data using the state-space model could construct a model with greater accuracy and flexibility than the ARIMA model. In this study, no seasonality or trend model was selected for any disease. It is thought that models with seasonality were not selected because diseases in swine shipped to slaughterhouses were the result of illness at some point during the 6-month fattening period between birth and shipment. Conclusion: Evaluation of previous diseases helps with the objective understanding of problems in fattening management. We believe that clarifying how farms manage fattening of their pigs will lead to improved farm profits. In that respect, it is important to use slaughterhouse data for fattening evaluation, and it is extremely useful to use mathematical models for slaughterhouse data. However, in this research, the model was constructed on the assumption of normality and linearity. In the future, we believe that we can build a more accurate model by considering models that assume non-normality and non-linearity.
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Notsu K., Hala E.D., Mitoma S., Wu X., Norimine J., Sekiguchi S.
mSphere 8 ( 4 ) 2023年8月
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Ngan M.T., Thi My Le H., Ngoc Dang V., Thi Bich Ngoc T., Phan L.V., Thi Hoa N., Quang Lam T., Thi Lan N., Notsu K., Sekiguchi S., Yamazaki Y., Yamazaki W.
Veterinary Medicine and Science 9 ( 3 ) 1226 - 1233 2023年5月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Veterinary Medicine and Science
Background: While early detection and early containment are key to controlling the African swine fever (ASF) pandemic, the lack of practical testing methods for use in the field are a major barrier to achieving this feat. Objectives: To describe the development of a rapid and sensitive point-of-care test (POCT) for ASF, and its evaluation using swine whole blood samples for field settings. Methods: In total, 89 swine whole blood samples were collected from Vietnamese swine farms and were performed the POCT using a combination of crude DNA extraction and LAMP (loop-mediated isothermal amplification) amplification. Results: The POCT enabled crude DNA to be extracted from swine whole blood samples within 10 min at extremely low cost and with relative ease. The entire POCT required a maximum of 50 min from the beginning of DNA extraction to final judgment. Compared to a conventional real-time PCR detection, the POCT showed a 1 log reduction in detection sensitivity, but comparable diagnostic sensitivity of 100% (56/56) and diagnostic specificity of 100% (33/33). The POCT was quicker and easier to perform and did not require special equipment. Conclusions: This POCT is expected to facilitate early diagnosis and containment of ASF invasion into both regions in which it is endemic and eradicated.
DOI： 10.1002/vms3.1124
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牛伝染性リンパ腫の新しい検査法とその利用 査読あり
関口 敏
畜産技術 2023 ( 816-May ) 36 - 40 2023年5月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：公益社団法人 畜産技術協会
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Corrigendum to "Development of droplet digital PCR for quantification of bovine leukemia virus proviral load using unpurified genomic DNA" [J. Virol. Methods, 315 (2023) 114706]. 査読あり
Wu X, Notsu K, Matsuura Y, Mitoma S, El Daous H, Norimine J, Sekiguchi S
Journal of virological methods 315 114708 2023年3月
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Dural Changes Induced by an Ultrasonic Bone Curette in an Excised Porcine Spinal Cord. 査読あり 国際共著
Ota R, Iwaki E, Sakai K, Haraguchi T, Kaneko Y, Sekiguchi S, Yamaguchi R, Naganobu K
Veterinary sciences 9 ( 11 ) 601 2022年10月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Veterinary Sciences
In spinal surgery, ultrasonic bone curettes are considered unlikely to cause mechanical injury to the dura; however, there is little evidence to support this claim. We investigated the effect of direct contact with an ultrasonic bone curette on the dura and the protective effect of covering the dura with a cotton pattie using an excised porcine spinal cord. The ultrasonic bone curette was pressed against the porcine spinal cord with constant force and activated for 1 s, with or without covering the dura with a cotton pattie. The dural surface and cross-section were observed using electron and light microscopy. When the ultrasonic bone curette was applied directly against the dura, most specimens showed non-perforating dural injuries. However, none of the specimens showed dural perforation. Histological changes were also observed. The use of a cotton pattie reduced the occurrence of these changes, although it did not prevent them when ultrasonic vibration was applied with a large force. We considered ultrasonic bone curettes to have a low risk of dural perforation and, thus, to be a safe surgical device as long as they did not accidentally make strong contact with the dura.