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Affiliation |
Center for Animal Disease Control |
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Title |
Associate Professor |
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External Link |
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MEKATA Hirohisa
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Research Areas 【 display / non-display 】
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Life Science / Veterinary medical science
Papers 【 display / non-display 】
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Generation of a Porcine Cell Line Stably Expressing Pig TMPRSS2 for Efficient Isolation of Swine Influenza Virus Reviewed International journal
Yuri L Tanaka, Maya Shofa, Erika P Butlertanaka, Ahmad Massoud Niazi, Takuya Hirai, Hirohisa Mekata, Akatsuki Saito
Pathogens 13 ( 1 ) 18 - 18 2023.12
Language：English Publishing type：Research paper (scientific journal) Publisher：MDPI AG
Pigs are important animals for meat production but can carry several zoonotic diseases, including the Japanese encephalitis virus, Nipah virus, and influenza viruses. Several Orthomyxoviridae and Coronavirinae respiratory viruses require cleavage of envelope proteins to acquire viral infectivity and consequently, need a host protease or the addition of exogenous trypsin for efficient propagation. Host TMPRSS2 is a key protease responsible for viral cleavage. Stable expression of human TMPRSS2 in African green monkey-derived Vero cells can enhance the porcine epidemic diarrhea virus. However, considering the narrow host tropism of viruses, a porcine cell line expressing pig TMPRSS2 could be optimal for replicating pig-derived viruses. Herein, we generated and evaluated a pig-derived PK-15 cell line stably expressing pig TMPRSS2. This cell line markedly (>1000-fold) and specifically enhanced the growth of influenza viruses. Furthermore, we demonstrated the usefulness of a PK-15 cell line lacking the Stat2 gene with a stable expression of pig TMPRSS2 for efficient virus isolation from clinical samples in the presence of type I interferons. Therefore, PK-15 cells expressing pig TMPRSS2 could be a valuable and promising tool for virus isolation, vaccine production, and virological studies of TMPRSS2-dependent viruses.
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Genetic and antigenic analyses of H5N8 and H5N1 subtypes high pathogenicity avian influenza viruses isolated from wild birds and poultry farms in Japan in the winter of 2021-2022. Reviewed International journal
Kosuke Soda, Hirohisa Mekata, Tatsufumi Usui, Hiroshi Ito, Yuto Matsui, Kentaro Yamada, Tsuyoshi Yamaguchi, Toshihiro Ito
The Journal of veterinary medical science 85 ( 11 ) 1180 - 1189 2023.9
Language：English Publishing type：Research paper (scientific journal)
In the winter of 2021-2022, multiple subtypes (H5N8 and H5N1) of high pathogenicity avian influenza viruses (HPAIVs) were confirmed to be circulating simultaneously in Japan. Here, we phylogenetically and antigenically analyzed HPAIVs that were isolated from infected wild birds, an epidemiological investigation of affected poultry farms, and our own active surveillance study. H5 subtype hemagglutinin (HA) genes of 32 representative HPAIV isolates were classified into clade 2.3.4.4b lineage and subsequently divided into three groups (G2a, G2b, and G2d). All H5N8 HPAIVs were isolated in early winter and had HA genes belonging to the G2a group. H5N1 HPAIVs belong to the G2b and G2d groups. Although G2b viruses were widespread throughout the season, G2d viruses endemically circulated in Northeast Japan after January 2022. Deep sequence analysis showed that the four HPAIVs isolated at the beginning of winter had both N8 and N1 subtypes of neuraminidase genes. Environmental water-derived G2a HPAIV, A/water/Tottori/NK1201-2/2021 (H5N8), has unique polymerase basic protein 1 and nucleoprotein genes, similar to those of low pathogenicity avian influenza viruses (LPAIVs). These results indicate that multiple H5 HPAIVs and LPAIVs disseminated to Japan via transboundary winter migration of wild birds, and HPAIVs with novel gene constellations could emerge in these populations. Cross-neutralization test revealed that G2a H5N8 HPAIVs were antigenically distinct from a G2b H5N1 HPAIV, suggesting that antibody pressure in wild birds was involved in the transition of the HPAIV groups during the season.
DOI： 10.1292/jvms.23-0121
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Detection and phylogenetic analysis of Dabieshan tick virus and Okutama tick virus in ticks collected from Cape Toi, Japan. Reviewed International journal
Hirohisa Mekata, Ikuo Kobayashi, Tamaki Okabayashi
Ticks and tick-borne diseases 14 ( 6 ) 102237 - 102237 2023.8
Authorship：Lead author, Corresponding author Language：English Publishing type：Research paper (scientific journal)
New technologies have led to the discovery of novel tick-borne and tick-associated viruses. Dabieshan tick virus (DaTV) and Okutama tick virus (OkTV), which belong to the family Phenuiviridae, were discovered in ticks in China and Japan, respectively, in the 2010s. Although it is unknown whether these viruses cause disease in animals or humans, all tick-associated viruses have the potential to become etiological agents of infectious diseases through gene reassortment. Therefore, it is important to elucidate the ecology of these viruses, regardless of their pathogenicity. In this study, ticks were collected year-round in Cape Toi, Miyazaki Prefecture, Japan, and an epidemiological survey of tick-associated phenuiviruses was performed. A total of 516 ticks collected from the vegetation by dragging flannel sheets were used for analysis. Pan-phenuivirus reverse transcription PCR was performed on the tick samples, and DaTV and OkTV were detected. We found that 37.0% (85/230) and 23% (16/71) of nymphal and adult Haemaphysalis longicornis were infected with DaTV, respectively, and 10% (6/62) and 13% (1/8) of nymphal and adult Haemaphysalis flava were infected with OkTV, respectively. Phylogenetic analysis indicated that the DaTV identified in this study formed a unique clade that was distinct from the strains identified in China. The survey revealed that DaTV is distributed not only in China, but also in Japan. We believe that this study contributes to our understanding of the prevalence of tick-associated viruses.
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Nosocomial Severe Fever with Thrombocytopenia Syndrome in Companion Animals, Japan, 2022 Reviewed International journal
Mekata H., Umeki K., Yamada K., Umekita K., Okabayashi T.
Emerging Infectious Diseases 29 ( 3 ) 614 - 617 2023.3
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal) Publisher：Emerging Infectious Diseases
In Japan, 2 cats that underwent surgery in a room where a sick dog had been euthanized became ill within 9 days of surgery. Severe fever with thrombocytopenia syndrome virus was detected in all 3 animals; nucleotide sequence identity was 100%. Suspected cause was an uncleaned pulse oximeter probe used for all patients.
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Seroprevalence of severe fever with thrombocytopenia syndrome virus in medium-sized wild mammals in Miyazaki, Japan Reviewed International coauthorship International journal
Chiho Kaneko, Hirohisa Mekata, Kazumi Umeki, Putu Eka Sudaryatma, Takao Irie, Kentaro Yamada, Naoaki Misawa, Kunihiko Umekita, Tamaki Okabayashi
Ticks and Tick-borne Diseases 14 ( 2 ) 102115 - 102115 2023.3
Language：English Publishing type：Research paper (scientific journal) Publisher：Elsevier BV
Books 【 display / non-display 】
MISC 【 display / non-display 】
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The Development of MALDI-ToF MS-based Analysis for the Direct Detection of SARS-CoV-2 in Nasopharyngeal Swabs
林克彦, 吉成知也, 廣瀬昌平, 大屋賢司, 大西貴弘, 渡辺麻衣子, 田原口智士, 目堅博久, 谷口隆秀, 前田卓哉, 折原悠太, 川村利江子, 新井沙倉, 斎藤嘉朗, 合田幸広, 工藤由起子
日本薬学会年会要旨集(Web) 143rd 2023
Language：Japanese Publishing type：Research paper, summary (national, other academic conference)
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牛伝染性リンパ腫の診断と早期発症予測法の開発
富永みその, 今内覚, 岡川朋弘, 神谷可菜, 齋藤麻矢, 安富一郎, 目堅博久, 前川直也, 村田史郎, 大橋和彦
北海道獣医師会雑誌 67 ( 8 ) 2023
Language：Japanese Publishing type：Research paper, summary (national, other academic conference)
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MALDI-ToF/MSによるSARS-CoV-2の新規検出法の開発
廣瀬昌平, 吉成知也, 林克彦, 大屋賢司, 大西貴弘, 渡辺麻衣子, 田原口智士, 目堅博久, 谷口隆秀, 新井沙倉, 合田幸広, 工藤由起子
日本獣医学会学術集会講演要旨集 165th (CD-ROM) 2022
Language：Japanese Publishing type：Research paper, summary (national, other academic conference)
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Encouragement of bovine leukemia control measure based on bovine leukemia virus proviral load
Hirohisa MEKATA
The Journal of Farm Animal in Infectious Disease 7 ( 4 ) 163 - 168 2018.12
Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (bulletin of university, research institution)
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Bovine leukemia virus infection and control measures in the field
Hirohisa MEKATA
The Yamaguchi journal of veterinary medicine 43 13 - 20 2016.12
Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (bulletin of university, research institution)
Presentations 【 display / non-display 】
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牛伝染性リンパ腫の防疫 Invited
目堅博久
北部九州しゃくなげ会 (熊本市) 2024.1.26
Event date： 2024.1.26
Language：Japanese Presentation type：Public lecture, seminar, tutorial, course, or other speech
Venue：熊本市 Country：Japan
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初乳給与を控えることは牛伝染性リンパ腫対策において重要ではない
目堅博久, 楠田絵梨子, 森千穂
第13回家畜感染症学会学術集会 2023.12.10
Event date： 2023.12.9 - 2023.12.10
Language：Japanese Presentation type：Oral presentation (general)
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初乳給与を控えることは 牛伝染性リンパ腫対策において重要ではない
目堅博久, 楠田絵梨子, 森千穂
第41回日本獣医師会獣医学術学会年次大会 2023.12.2
Event date： 2023.12.1 - 2023.12.3
Language：Japanese Presentation type：Oral presentation (general)
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Single-Nucleotide Polymorphism on Spermatogenesis Associated 16 Gene-Coding Region Affecting Bovine Leukemia Virus Proviral Load International conference
Hirohisa Mekata, Mari Yamamoto
21st Federation of Asian Veterinary Associations (FAVA) Congress 2022.11.13
Event date： 2022.11.11 - 2022.11.13
Language：English Presentation type：Oral presentation (general)
Country：Japan
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牛のコロナウイルス：わが国の流行状況と呼吸器病における役割 Invited
目堅 博久, Putu Sudaryatma, 齊藤 暁, 岡林 環樹
第163回日本獣医学会学術集会 微生物学分科会シンポジウム (web開催) 2020.9.14 日本獣医学会
Event date： 2020.9.14 - 2020.9.30
Language：Japanese Presentation type：Oral presentation (invited, special)
Venue：web開催 Country：Japan
Awards 【 display / non-display 】
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日本獣医学会獣医学奨励賞
2013.3 日本獣医学会
目堅 博久
Award type：Award from Japanese society, conference, symposium, etc. Country：Japan
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第１５５回日本獣医学会大会長賞
2013.3 日本獣医学会
目堅 博久
Award type：Award from Japanese society, conference, symposium, etc. Country：Japan
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日本産業動物獣医学会九州地区学会長賞
2014.10 日本産業動物獣医学会
目堅 博久
Award type：Award from Japanese society, conference, symposium, etc. Country：Japan
Grant-in-Aid for Scientific Research 【 display / non-display 】
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ベトナムにおけるアフリカ豚熱の実践的な高感度診断法の開発と環境動態の解明
Grant number：22KK009701 2022.04 - 2027.03
独立行政法人日本学術振興会 科学研究費補助金 国際共同研究加速基金(国際共同研究強化(B))
Authorship：Coinvestigator(s)
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Construction of animal foamy virus vector and development of enzootic bovine leukosis vaccine
Grant number：22H02500 2022.04 - 2026.03
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
Authorship：Principal investigator
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Studies on anti-tumor potential of symbiotic retroviruses and their therapeutic applications
Grant number：20H03150 2020.04 - 2024.03
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
Authorship：Coinvestigator(s)
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Pathological analysis of nasopharynx and lung caused by respiratory disease virus in pigs
Grant number：18K05974 2018.04 - 2021.03
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
Hirai Takuya
Authorship：Coinvestigator(s)
Swine influenza virus (A/H1N1pdm09) were detected in the piglets of the local pig farm in Miyazaki prefecture. Subclinical infection was considered because these piglets did not show respiratory sign. CK-18 immunohistochemistry demonstrated M cells in the epithelial cells covering nasal-associated lymphoid tissue (NALT). In addition, lectin histochemistry revealed that the number of α2,6-linked sialic acid is more than α2,3-linked sialic acid in the epithelial cells of NALT. Several epithelial cells show double positive for both CK-18 immunohistochemistry and α2,6-linked sialic acid lectin histochemistry. Influenza positive macrophages were also detected in the NALT. These results suggest that M cells express α2,6-linked sialic acid, and influenza virus transported by M cells may be engulfed by macrophages or dendritic cells.
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市町村単位での牛白血病対策プログラムの確立に向けた実証研究
2017.04 - 2019.03
科学研究費補助金 若手研究(B)
Authorship：Principal investigator