MEKATA Hirohisa

写真a

Affiliation

Center for Animal Disease Control

Title

Associate Professor

External Link

Degree 【 display / non-display

  • 獣医学博士 ( 2012.3   北海道大学 )

Research Areas 【 display / non-display

  • Life Science / Veterinary medical science

 

Papers 【 display / non-display

  • Nosocomial Severe Fever with Thrombocytopenia Syndrome in Companion Animals, Japan, 2022 Reviewed International journal

    Mekata H., Umeki K., Yamada K., Umekita K., Okabayashi T.

    Emerging Infectious Diseases   29 ( 3 )   614 - 617   2023.3

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:Emerging Infectious Diseases  

    In Japan, 2 cats that underwent surgery in a room where a sick dog had been euthanized became ill within 9 days of surgery. Severe fever with thrombocytopenia syndrome virus was detected in all 3 animals; nucleotide sequence identity was 100%. Suspected cause was an uncleaned pulse oximeter probe used for all patients.

    DOI: 10.3201/eid2903.220720

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  • Seroprevalence of severe fever with thrombocytopenia syndrome virus in medium-sized wild mammals in Miyazaki, Japan Reviewed International coauthorship International journal

    Chiho Kaneko, Hirohisa Mekata, Kazumi Umeki, Putu Eka Sudaryatma, Takao Irie, Kentaro Yamada, Naoaki Misawa, Kunihiko Umekita, Tamaki Okabayashi

    Ticks and Tick-borne Diseases   14 ( 2 )   102115 - 102115   2023.3

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Elsevier BV  

    DOI: 10.1016/j.ttbdis.2022.102115

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  • Possible transmission of severe fever with thrombocytopenia syndrome virus (SFTSV) to a person who buried the corpus of a dead cat, which died of SFTSV infection. Reviewed International journal

    Hirohisa Mekata, Takeshi Kawaguchi, Kosho Iwao, Kazumi Umeki, Kentaro Yamada, Kunihiko Umekita, Tamaki Okabayashi

    Japanese journal of infectious diseases   2023.1

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    Severe fever with thrombocytopenia syndrome virus (SFTSV) is a causative agent of SFTS. Although SFTS is a fatal tick-borne zoonosis, the virus can infect humans without tick bite exposure. Recently, direct SFTSV transmission from companion pets to humans has become a significant problem. Herein, we reported a case of SFTSV transmission from a dead community cat to a woman who buried the cat in Miyazaki prefecture, Japan. The community cat died without a diagnosis of SFTS, and the woman buried it without precaution. Nine days later, the woman developed symptoms of SFTS. SFTS viral RNA and anti-SFTSV antibodies were detected in the woman. The animal carcass was exhumed, and tissue samples were collected to confirm viral infection in the cat. High copy numbers of viral RNA were detected. Sequences of the SFTSV M segment in samples from the cat and the woman showed 100% homology. The woman stated that she had touched blood that had leaked from the body when she buried it. On the other hand, the woman could have been infected when she took the cat to the animal hospital. This study highlights the risk of SFTSV infection via contact with sick or dead community cats.

    DOI: 10.7883/yoken.JJID.2022.425

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  • Generation of a bovine cell line for gene engineering using an HIV-1-based lentiviral vector. Reviewed International journal

    Nanami Morizako, Erika P Butlertanaka, Yuri L Tanaka, Honoka Shibata, Tamaki Okabayashi, Hirohisa Mekata, Akatsuki Saito

    Scientific reports   12 ( 1 )   16952 - 16952   2022.10

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    Language:English   Publishing type:Research paper (scientific journal)  

    Human immunodeficiency virus type 1 (HIV-1)-based lentiviral vectors are indispensable tools for gene engineering in mammalian cells. Conversely, lentiviral vector transduction is severely inhibited in bovine cells. Previous studies demonstrated that this inhibition is caused by the anti-lentiviral host factor tripartite motif containing 5 (TRIM5), which targets incoming HIV-1 virions by interacting with the viral capsid. In this study, we investigated several methods for overcoming the limited applicability of lentiviral vectors in bovine cells. First, we demonstrated that the SPRY domain of bovine TRIM5 is the major determinant of anti-viral activity. Second, we found that mutations that allow the capsid to evade rhesus macaque TRIM5α minimally rescued HIV-1 infectivity in bovine-derived MDBK cells. Third, we found that cyclosporine A, which relieves the inhibition of HIV-1 infection in monkey cells, significantly rescued the impaired HIV-1 infectivity in MDBK cells. Lastly, we successfully generated a bovine cell line lacking intact TRIM5 using the CRISPR/Cas9 technique. This TRIM5 knockout cell line displayed significantly higher susceptibility to an HIV-1-based lentiviral vector. In conclusion, our findings provide a promising gene engineering strategy for bovine cells, thereby contributing to innovations in agriculture and improvements in animal health.

    DOI: 10.1038/s41598-022-20970-6

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  • 黒毛和種牛における牛伝染性リンパ腫ウイルスの垂直感染の発生状況とリスク要因 Reviewed

    楠田 絵梨子, 浅野 康子, 米重 隆一, 目堅 博久

    家畜診療   69 ( 9 )   527 - 532   2022.9

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    Authorship:Last author   Language:Japanese   Publishing type:Research paper (scientific journal)   Publisher:全国農業共済協会  

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Books 【 display / non-display

  • 畜産現場のバイオセキュリティ

    目堅博久(牛白血病のバイオセキュリティ)

    緑書房  2019.7 

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    Language:Japanese

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  • Encouragement of bovine leukemia control measure based on bovine leukemia virus proviral load

    Hirohisa MEKATA

    The Journal of Farm Animal in Infectious Disease   7 ( 4 )   163 - 168   2018.12

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    Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (bulletin of university, research institution)  

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  • Bovine leukemia virus infection and control measures in the field

    Hirohisa MEKATA

    The Yamaguchi journal of veterinary medicine   43   13 - 20   2016.12

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    Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (bulletin of university, research institution)  

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  • Prognosis evaluation of high-viral-load BLV-infected cattle

    Hirohisa MEKATA

    34   231 - 236   2016.11

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    Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (bulletin of university, research institution)  

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  • D型インフルエンザウイルスはわが国のウシ社会に侵淫している

    堀本泰介, 日尾野隆大, 目堅博久, 小田切友葉, 雷志皓, 遠藤麻衣子, 上間亜希子, 小林知也, CHAMBERS James, 内田和幸, 西原真杉, 乗峰潤三, 猪島康雄, 彦野弘一, 村上賢二, 佐藤礼一郎, 村上裕信, 阪口雅弘, 安藤貴朗, 乙丸孝之介, 小澤真, 石井一功, 迫田義博, 村上晋

    日本獣医学会学術集会講演要旨集   159th   386 - 386   2016.8

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    Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (bulletin of university, research institution)   Publisher:(公社)日本獣医学会  

    J-GLOBAL

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  • Bovine leukemia virus infection routes and control measures in the field

    Hirohisa MEKATA

    Japanese Journal of Large Animal Clinics   6 ( 3 )   133 - 140   2015.11

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    Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (bulletin of university, research institution)  

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Presentations 【 display / non-display

  • Single-Nucleotide Polymorphism on Spermatogenesis Associated 16 Gene-Coding Region Affecting Bovine Leukemia Virus Proviral Load International conference

    Hirohisa Mekata, Mari Yamamoto

    21st Federation of Asian Veterinary Associations (FAVA) Congress  2022.11.13 

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    Event date: 2022.11.11 - 2022.11.13

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

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  • 牛のコロナウイルス:わが国の流行状況と呼吸器病における役割 Invited

    目堅 博久, Putu Sudaryatma, 齊藤 暁, 岡林 環樹

    第163回日本獣医学会学術集会 微生物学分科会シンポジウム  (web開催)  2020.9.14  日本獣医学会

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    Event date: 2020.9.14 - 2020.9.30

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:web開催   Country:Japan  

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  • 国内で流行するウシ呼吸器コロナウイルスの分子疫学調査と系統解析

    目堅博久, 浜辺早織, 岡林環樹

    令和元年度 日本獣医師会獣医学術学会年次大会  (東京国際フォーラム)  2020.2.8 

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    Event date: 2020.2.8

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:東京国際フォーラム  

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  • Bovine leukemia virus infection in Japan and the control strategy based on the proviral load Invited International conference

    Hirohisa MEKATA

    Joint Conference on Bovine Medicine of University of Edinburgh and Hokkaido University  2019.8.20 

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    Event date: 2019.8.20

    Language:English   Presentation type:Public lecture, seminar, tutorial, course, or other speech  

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  • フィールドにおけるTheileria orientalisの母子間伝播リスク Invited

    目堅博久

    牛臨床寄生虫研究会  (霧島市)  2019.6.30  牛臨床寄生虫研究会

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    Event date: 2019.6.30

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:霧島市  

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Awards 【 display / non-display

  • 日本獣医学会獣医学奨励賞

    2013.3   日本獣医学会  

    目堅 博久

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    Award type:Award from Japanese society, conference, symposium, etc.  Country:Japan

  • 第155回日本獣医学会大会長賞

    2013.3   日本獣医学会  

    目堅 博久

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    Award type:Award from Japanese society, conference, symposium, etc.  Country:Japan

  • 日本産業動物獣医学会九州地区学会長賞

    2014.10   日本産業動物獣医学会  

    目堅 博久

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    Award type:Award from Japanese society, conference, symposium, etc.  Country:Japan

Grant-in-Aid for Scientific Research 【 display / non-display

  • ベトナムにおけるアフリカ豚熱の実践的な高感度診断法の開発と環境動態の解明

    Grant number:22KK009701  2022.04 - 2027.03

    科学研究費補助金  国際共同研究加速基金(国際共同研究強化(B))

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    Authorship:Coinvestigator(s) 

  • 動物フォーミーウイルスベクターの基盤構築と牛伝染性リンパ腫ワクチンの開発

    Grant number:22H02500  2022.04 - 2026.03

    日本学術振興会  科学研究費助成事業 基盤研究(B)  基盤研究(B)

    目堅 博久

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    Authorship:Principal investigator 

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  • 共生レトロウイルスの抗腫瘍ポテンシャルの解明と有効活用

    Grant number:20H03150  2020.04 - 2024.03

    独立行政法人日本学術振興会  科学研究費補助金  基盤研究(B)

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  • 市町村単位での牛白血病対策プログラムの確立に向けた実証研究

    2017.04 - 2019.03

    科学研究費補助金  若手研究(B)

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    Authorship:Principal investigator 

  • 牛白血病ウイルスの垂直伝播と発症リスクに関する研究

    2014.04 - 2017.03

    科学研究費補助金  若手研究(B)

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    Authorship:Principal investigator 

    牛白血病ウイルスの垂直伝播と発症リスクに関する研究を行う。

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