MEKATA Hirohisa

写真a

Affiliation

Center for Animal Disease Control

Title

Associate Professor

External Link

Degree 【 display / non-display

  • 獣医学博士 ( 2012.3   北海道大学 )

Research Areas 【 display / non-display

  • Life Science / Veterinary medical science

 

Papers 【 display / non-display

  • Single-Nucleotide Polymorphism on Spermatogenesis Associated 16 Gene-Coding Region Affecting Bovine Leukemia Virus Proviral Load. Reviewed International journal

    Hirohisa Mekata, Mari Yamamoto

    Veterinary sciences   9 ( 6 )   2022.6

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    Authorship:Lead author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    Bovine leukemia virus (BLV) is an etiological agent of malignant lymphoma in cattle and is endemic in many cattle-breeding countries. Thus, the development of cattle genetically resistant to BLV is desirable. The purpose of this study was to identify novel single-nucleotide polymorphisms (SNPs) related to resistance to BLV. A total of 146 DNA samples from cattle with high BLV proviral loads (PVLs) and 142 samples from cattle with low PVLs were used for a genome-wide association study (GWAS). For the verification of the GWAS results, an additional 1342 and 456 DNA samples from BLV-infected Japanese Black and Holstein cattle, respectively, were used for an SNP genotyping PCR to compare the genotypes for the identified SNPs and PVLs. An SNP located on the spermatogenesis associated 16 (SPATA16)-coding region on bovine chromosome 1 was found to exceed the moderate threshold (p < 1.0 × 10-5) in the Additive and Dominant models of the GWAS. The SNP genotyping PCR revealed that the median values of the PVL were 1278 copies/50 ng of genomic DNA for the major homozygous, 843 for the heterozygous, and 621 for the minor homozygous genotypes in the Japanese Black cattle (p < 0.0001). A similar tendency was also observed in the Holstein cattle. We found that cattle with the minor allele for this SNP showed 20-25% lower PVLs. Although the mechanisms through which this SNP impacts the PVL remain unknown, we found a novel SNP related to BLV resistance located on the SPATA16 gene-coding region on bovine chromosome 1.

    DOI: 10.3390/vetsci9060275

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  • Survival of SARS-CoV-2 and bovine coronavirus on common surfaces of living environments. Reviewed International journal

    Maiko Watanabe, Takahiro Ohnishi, Sakura Arai, Tsuyoshi Kawakami, Katsuhiko Hayashi, Kenji Ohya, Shouhei Hirose, Tomoya Yoshinari, Satoshi Taharaguchi, Hirohisa Mekata, Takahide Taniguchi, Yoshiaki Ikarashi, Masamitsu Honma, Yukihiro Goda, Yukiko Hara-Kudo

    Scientific reports   12 ( 1 )   10624 - 10624   2022.6

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    Language:English   Publishing type:Research paper (scientific journal)  

    Aerosols or saliva containing severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) can contaminate living environments, and viruses can be indirectly transmitted. To understand the survival potential of the virus, the viral titers of bovine coronavirus (BCoV), as a model virus, and SARS-CoV-2 were measured on porous and non-porous surfaces. The amount of infectious BCoV recovered remained relatively high on non-porous substrates. However, it quickly decreased on several non-porous surfaces such as nitrile rubber. The time taken to reach the limit of detection on non-woven masks, as a porous substrate, was longer than that of non-porous substrates. On porous substrates other than non-woven masks, the amount of virus recovered quickly decreased, and then remained at a low level. Representative substrates were tested with SARS-CoV-2. The decrease in the amount of infectious virus recovered was similar to that of BCoV, although that of SARS-CoV-2 was more rapid. RNA derived from SARS-CoV-2 was also detected using real-time PCR, and it remained on surfaces much longer than infectious virus, on all substrates. Therefore, it is important to measure the viral titer to avoid the overestimation of infectious virus contamination in the environments. Our results suggest that the surface structure was not directly related to viral survivability.

    DOI: 10.1038/s41598-022-14552-9

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  • Development of a Direct Polymerase Chain Reaction Method for Detecting Major Sources of Bovine Leukemia Virus Infection Reviewed

    Machiko MIYAMOTO, Kyoko KAWAUCHI, Shinichi SAKAKIBARA, Kazuhiro MIYANE, Hirohisa MEKATA

    Journal of the Japan Veterinary Medical Association   75 ( 3 )   e46 - e50   2022.3

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    Authorship:Last author   Language:Japanese   Publishing type:Research paper (scientific journal)  

    DOI: https://doi.org/10.12935/jvma.75.e46

  • Matrix-Assisted Laser Desorption and Ionization Time-of-Flight Mass Spectrometry Analysis for the Direct Detection of SARS-CoV-2 in Nasopharyngeal Swabs. Reviewed International journal

    Tomoya Yoshinari, Katsuhiko Hayashi, Shouhei Hirose, Kenji Ohya, Takahiro Ohnishi, Maiko Watanabe, Satoshi Taharaguchi, Hirohisa Mekata, Takahide Taniguchi, Takuya Maeda, Yuta Orihara, Rieko Kawamura, Sakura Arai, Yoshiro Saito, Yukihiro Goda, Yukiko Hara-Kudo

    Analytical chemistry   94 ( 10 )   4218 - 4226   2022.3

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    The most common diagnostic method used for coronavirus disease-2019 (COVID-19) is real-time reverse transcription polymerase chain reaction (PCR). However, it requires complex and labor-intensive procedures and involves excessive positive results derived from viral debris. We developed a method for the direct detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in nasopharyngeal swabs, which uses matrix-assisted laser desorption and ionization time-of-flight mass spectrometry (MALDI-ToF MS) to identify specific peptides from the SARS-CoV-2 nucleocapsid phosphoprotein (NP). SARS-CoV-2 viral particles were separated from biological molecules in nasopharyngeal swabs by an ultrafiltration cartridge. Further purification was performed by an anion exchange resin, and purified NP was digested into peptides using trypsin. The peptides from SARS-CoV-2 that were inoculated into nasopharyngeal swabs were detected by MALDI-ToF MS, and the limit of detection was 106.7 viral copies. This value equates to 107.9 viral copies per swab and is approximately equivalent to the viral load of contagious patients. Seven NP-derived peptides were selected as the target molecules for the detection of SARS-CoV-2 in clinical specimens. The method detected between two and seven NP-derived peptides in 19 nasopharyngeal swab specimens from contagious COVID-19 patients. These peptides were not detected in four specimens in which SARS-CoV-2 RNA was not detected by PCR. Mutated NP-derived peptides were found in some specimens, and their patterns of amino acid replacement were estimated by accurate mass. Our results provide evidence that the developed MALDI-ToF MS-based method in a combination of straightforward purification steps and a rapid detection step directly detect SARS-CoV-2-specific peptides in nasopharyngeal swabs and can be a reliable high-throughput diagnostic method for COVID-19.

    DOI: 10.1021/acs.analchem.1c04328

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  • Avoidance of Natural Suckling from Dams with Bovine Leukemia Virus Is a Low Priority Countermeasure against Postnatal Transmission Reviewed International journal

    Hirohisa Mekata, Eriko Kusuda, Chiho Mori

    Veterinary Sciences   8 ( 11 )   255 - 255   2021.10

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    Authorship:Lead author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:MDPI AG  

    Although natural suckling from dams with bovine leukemia virus (BLV) has not been recommended in Japan, the frequency of BLV transmission through natural suckling under natural conditions is still unclear. The purpose of this study was to elucidate the risk of BLV transmission through natural suckling. Dams with BLV were classified into three groups (high, middle, low) based on the proviral loads (PVLs). PCR positivity of their colostrum and the correlations between the ratios of calves with BLV and types of feeding milk were analyzed. In dams with low PVLs, no colostrum or calves were confirmed to have BLV. In dams with middle and high PVLs, 17 out of 25 (68.0%) colostrum were PCR positive, and 10 out of 23 (43.4%) and 13 out of 29 (44.8%) calves with natural suckling and artificial rearing were infected with BLV, respectively. No difference was confirmed between the infection rates of natural-suckled and artificially reared calves. Thus, we concluded that the avoidance of natural suckling from dams with BLV and the introduction of artificial rearing were low priority countermeasures against BLV transmission.

    DOI: 10.3390/vetsci8110255

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Books 【 display / non-display

  • 畜産現場のバイオセキュリティ

    目堅博久(牛白血病のバイオセキュリティ)

    緑書房  2019.7 

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    Language:Japanese

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  • Encouragement of bovine leukemia control measure based on bovine leukemia virus proviral load

    Hirohisa MEKATA

    The Journal of Farm Animal in Infectious Disease   7 ( 4 )   163 - 168   2018.12

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    Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (bulletin of university, research institution)  

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  • Bovine leukemia virus infection and control measures in the field

    Hirohisa MEKATA

    The Yamaguchi journal of veterinary medicine   43   13 - 20   2016.12

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    Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (bulletin of university, research institution)  

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  • Prognosis evaluation of high-viral-load BLV-infected cattle

    Hirohisa MEKATA

    34   231 - 236   2016.11

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  • D型インフルエンザウイルスはわが国のウシ社会に侵淫している

    堀本泰介, 日尾野隆大, 目堅博久, 小田切友葉, 雷志皓, 遠藤麻衣子, 上間亜希子, 小林知也, CHAMBERS James, 内田和幸, 西原真杉, 乗峰潤三, 猪島康雄, 彦野弘一, 村上賢二, 佐藤礼一郎, 村上裕信, 阪口雅弘, 安藤貴朗, 乙丸孝之介, 小澤真, 石井一功, 迫田義博, 村上晋

    日本獣医学会学術集会講演要旨集   159th   386 - 386   2016.8

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    Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (bulletin of university, research institution)   Publisher:(公社)日本獣医学会  

    J-GLOBAL

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  • Bovine leukemia virus infection routes and control measures in the field

    Hirohisa MEKATA

    Japanese Journal of Large Animal Clinics   6 ( 3 )   133 - 140   2015.11

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Presentations 【 display / non-display

  • 牛のコロナウイルス:わが国の流行状況と呼吸器病における役割 Invited

    目堅 博久, Putu Sudaryatma, 齊藤 暁, 岡林 環樹

    第163回日本獣医学会学術集会 微生物学分科会シンポジウム  (web開催)  2020.9.14  日本獣医学会

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    Event date: 2020.9.14 - 2020.9.30

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:web開催   Country:Japan  

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  • 国内で流行するウシ呼吸器コロナウイルスの分子疫学調査と系統解析

    目堅博久, 浜辺早織, 岡林環樹

    令和元年度 日本獣医師会獣医学術学会年次大会  (東京国際フォーラム)  2020.2.8 

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    Event date: 2020.2.8

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:東京国際フォーラム  

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  • Bovine leukemia virus infection in Japan and the control strategy based on the proviral load Invited International conference

    Hirohisa MEKATA

    Joint Conference on Bovine Medicine of University of Edinburgh and Hokkaido University  2019.8.20 

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    Event date: 2019.8.20

    Language:English   Presentation type:Public lecture, seminar, tutorial, course, or other speech  

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  • フィールドにおけるTheileria orientalisの母子間伝播リスク Invited

    目堅博久

    牛臨床寄生虫研究会  (霧島市)  2019.6.30  牛臨床寄生虫研究会

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    Event date: 2019.6.30

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:霧島市  

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  • Bovine leukemia virus infection in Japan and the control strategy based on the proviral load Invited International conference

    Hirohisa MEKATA

    The 2nd Joint Symposium between CADIC in UoM and FACTRC in SNU  (Pyeongchang, Republic of Korea )  2019.3.26  Seoul National University

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    Event date: 2019.3.26

    Language:Japanese   Presentation type:Public lecture, seminar, tutorial, course, or other speech  

    Venue:Pyeongchang, Republic of Korea  

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Awards 【 display / non-display

  • 日本獣医学会獣医学奨励賞

    2013.3   日本獣医学会  

    目堅 博久

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    Award type:Award from Japanese society, conference, symposium, etc.  Country:Japan

  • 第155回日本獣医学会大会長賞

    2013.3   日本獣医学会  

    目堅 博久

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    Award type:Award from Japanese society, conference, symposium, etc.  Country:Japan

  • 日本産業動物獣医学会九州地区学会長賞

    2014.10   日本産業動物獣医学会  

    目堅 博久

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    Award type:Award from Japanese society, conference, symposium, etc.  Country:Japan

Grant-in-Aid for Scientific Research 【 display / non-display

  • 動物フォーミーウイルスベクターの基盤構築と牛伝染性リンパ腫ワクチンの開発

    Grant number:22H02500  2022.04 - 2026.03

    日本学術振興会  科学研究費助成事業 基盤研究(B)  基盤研究(B)

    目堅 博久

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    Authorship:Principal investigator 

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  • 共生レトロウイルスの抗腫瘍ポテンシャルの解明と有効活用

    Grant number:20H03150  2020.04 - 2024.03

    科学研究費補助金  基盤研究(B)

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    Authorship:Coinvestigator(s) 

  • 市町村単位での牛白血病対策プログラムの確立に向けた実証研究

    2017.04 - 2019.03

    科学研究費補助金  若手研究(B)

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    Authorship:Principal investigator 

  • 牛白血病ウイルスの垂直伝播と発症リスクに関する研究

    2014.04 - 2017.03

    科学研究費補助金  若手研究(B)

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    牛白血病ウイルスの垂直伝播と発症リスクに関する研究を行う。

  • トリパノソーマ原虫における薬剤耐性機序および病原性規定遺伝子に関する研究

    2009.04 - 2012.03

    科学研究費補助金  特別研究員推奨費

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    Authorship:Principal investigator 

    フィリピンにおけるTrypanosoma evansiの疫学調査と病原性因子の特定、宿主免疫機構の解析