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Faculty of Agriculture Region of Animal and Plant Biosciences |
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Professor |
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Related SDGs |
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ZUSHI Kazufumi
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Research Areas 【 display / non-display 】
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Environmental Science/Agriculture Science / Horticultural science
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Environmental Science/Agriculture Science / Agricultural environmental engineering and agricultural information engineering
Papers 【 display / non-display 】
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Zushi K., Yamamoto M., Matsuura M., Tsutsuki K., Yonehana A., Imamura R., Takahashi H., Kirimura M.
Journal of the Science of Food and Agriculture 105 ( 2 ) 1159 - 1169 2024.9
Authorship：Corresponding author Publishing type：Research paper (scientific journal) Publisher：Journal of the Science of Food and Agriculture
BACKGROUND: Strawberry is a rich source of antioxidants, including ascorbic acid (ASA) and polyphenols, which have numerous health benefits. Antioxidant content and activity are often determined manually using laboratory equipment, which is destructive and time-consuming. This study constructs a prediction model for antioxidant compounds utilizing machine learning (ML) and multiple linear regression based on environmental, plant growth and agronomic fruit quality-related parameters as well as antioxidant levels. These were studied in three farms at two-week intervals during two years of cultivation. RESULTS: During the ML model screening, artificial neural network (ANN)-boosted models displayed a moderate coefficient of determination (R2) at 0.68–0.78 and relative root mean square error (RRMSE) at 3.8–4.8% in polyphenols and total ASA levels, as well as a high R2 of 0.96 and low RRMSE at <3.0% in antioxidant activity. Additionally, we developed variable selection models regarding the antioxidant activity, and variables two and five (environmental parameters and leaf length, respectively) with high accuracy were selected. The linear regression analysis between the actual and predicted data of antioxidants in the ANN-boosted models revealed high fitness with all parameters in almost all training, validation and test sets. Furthermore, environmental parameters are essential in developing such reliable models. CONCLUSION: We conclude that ANN-boosted, stepwise and double-Lasso regression models can predict antioxidant compounds with enhanced accuracy, and the relevant parameters can be easily acquired on-site without the need for any specific equipment. © 2024 Society of Chemical Industry.
DOI： 10.1002/jsfa.13906
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Postharvest changes in texture profiles and pectin metabolism in salt-stressed tomato Reviewed
Zushi K., Hojima M., Higashijima M.
Scientia Horticulturae 336 2024.10
Authorship：Corresponding author Publishing type：Research paper (scientific journal) Publisher：Scientia Horticulturae
In tomato cultivation, applying salt stress has been found to enhance organoleptic qualities. The texture of the tomato fruit is a crucial factor in determining quality and shelf life. However, postharvest changes in fruit texture of tomato grown under salt stress have not been studied. The study aimed to explore these changes and their relationship with pectin content and the activity of pectin-metabolizing enzymes. Salt stress was induced through addition of 100 mM NaCl to the nutrient solution, and ripe fruits were stored at 5 °C for 14 days. The loss of fruit weight after storage was consistent in both control and salt-stressed fruits, salt stress reduced the loss of fruit firmness by 36 % compared to control fruit. Salt-stressed fruits exhibited minor postharvest changes in several texture profiles compared to control fruits. Moreover, the mitigation of postharvest texture softening in salt-stressed fruits varied across different fruit tissues. Salt stress 40 % to 100 % increased the pectin content, which remained high during storage. Additionally, pectin-metabolizing enzymes like pectin methylesterase (PME) and polygalacturonase (PG) activities were 140 % and 80 % increased by salt stress, respectively; however, PG activity decreased during storage in salt-stressed fruits. The adhesiveness of the pericarp tissue was correlated with water-soluble pectin, PME, and PG. Therefore, we conclude that salt stress may mitigate postharvest texture softening, and the higher pectin content and pectin-metabolizing enzymes may contribute to this reduction in texture softening during storage in salt-stressed fruits. These findings suggest that salt stress could be a viable method for enhancing the texture and shelf life of tomatoes.
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Nakamura T., Zushi K.
Acta Horticulturae 3 ( 1404 ) 841 - 848 2024.9
Authorship：Last author Publishing type：Research paper (scientific journal) Publisher：Acta Horticulturae
Lutein is an antioxidant known to promote good health, and it is found in high concentrations in spinach leaves. Spinach is grown specifically for frozen processing in certain regions of Japan. However, there is a limited understanding of variation factors that affect the lutein content of spinach for frozen processing. Therefore, to gain a better understanding of these factors, we analyzed the relationship between the lutein content of spinach, environmental conditions, growth traits (flower bud differentiation, leaf numbers, plant height and weight, SPAD value, and sugar content), and chlorophyll a fluorescence OJIP transients to identify the factors that influence the lutein content. During the cultivation period (October 13, 2020 to April 1, 2021), lutein content, growth traits, and OJIP parameters were measured every month until flowering. Air temperature, solar radiation, and photoperiod increased from February until the end of the measurement period. Lutein content was approximately 6.6 mg 100 g-1 from November to January, but it decreased significantly to 3.5 mg 100 g-1 in February and March. Although the lutein content did not correlate with all of the growth traits, the period during which the lutein content decreased was coincident with the period from the flower bud differentiation of an apical meristem to bolting. Furthermore, the maximum fluorescence intensity in the OJIP was the highest in February, and the energy dissipation flux of the OJIP parameters was the highest in February. Therefore, the development of flower buds and the high energy dissipation in the photosynthetic apparatus with increased solar radiation may decrease in lutein content in February and March. In conclusion, we found that the development of flower buds may contribute to the decrease in lutein content and that the OJIP parameter may serve as an indicator of variation in the lutein content of spinach for frozen processing.
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ZUSHI Kazufumi, SUEHARA Chisato, SHIRAI Minori
Environment Control in Biology 62 ( 2 ) 49 - 56 2024.4
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：Japanese Society of Agricultural, Biological and Environmental Engineers and Scientists
Ascorbic acid (AsA) is an essential phytonutrient, and tomatoes are an important dietary source. Tomato fruit AsA content is regulated by light conditions and is cultivar-dependent, but their regulations is unknown about the role of source-sink relationships. This study was designed to compare the light/dark-controlled regulation of AsA between intact (plant-attached fruits with source-sink relationships) and <i>in vitro</i> culture (plant-detached fruits without source-sink relationships) tomato fruits of five cultivars. Compared with fruits grown under light conditions, AsA content was lower in both intact and <i>in vitro</i> fruits grown in the dark; however, the decrease in AsA in the dark treatment was markedly lower in intact fruits (17-29%) than <i>in vitro</i> fruits (44-74%) and was dependent on the cultivar. Antioxidant enzyme activities and lipid peroxidation were lower under the dark condition in <i>in vitro</i> fruits (30-90%) but not intact fruits. Therefore, the reduction of AsA content under darkness occurred due to decreased <i>in situ</i> biosynthesis and antioxidant system activity in fruit without source-sink relationships. Furthermore, the reduction of AsA content induced by darkness was mitigated by source-sink relationships, as indicated by the intact fruit results, and their effects depend on cultivars.
DOI： 10.2525/ecb.62.49
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Nakamura Takeshi, Zushi Kazufumi
Horticultural Research (Japan) 23 ( 4 ) 261 - 269 2024
Authorship：Last author Language：Japanese Publishing type：Research paper (scientific journal) Publisher：THE JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE
Lutein, known for its health benefits, is abundant in spinach (<i>Spinacia oleracea</i>); however, factors influencing its concentration remain unclear in spinach for processing. Therefore, in this study, the relationships between the variation in lutein concentrations with the season and developmental stages, and agronomic parameters, such as weather conditions, growth traits, and chlorophyll a fluorescence OJIP transients (OJIP transients), were investigated. Spinach was cultivated until flowering in autumn and winter sowing. Seasonal variation in the lutein concentration was categorized into increasing and decreasing phases. The concentration increased at low temperatures and decreased at the optimal growth temperature, indicating a negative correlation with atmospheric temperature. During the increasing phases, OJIP transient levels (Fv/Fo) decreased due to chilling stress–induced growth inhibition. During the decreasing phases, prolonged chilling stress over 2 months led to reductions in OJIP transient levels, such as the performance index and Fv/Fm. Notably, initiation of the decreasing phase coincided with the flower stalk elongation period. This suggests a direct effect of atmospheric temperature on the lutein concentration in spinach, with flower stalk elongation potentially contributing to lower concentrations. Furthermore, OJIP transients emerged as potential indicators for monitoring the lutein concentration in spinach for processing.
DOI： 10.2503/hrj.23.261
Books 【 display / non-display 】
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機能性成分が秘めるビジネスチャンス 各社事例から学ぶ成分向上手法/事業活用例
圖師一文（ Role： Sole author , 塩ストレスによるトマト内ポリフェノール向上）
株式会社情報機構 2016.3
Language：Japanese Book type：Scholarly book
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総合生活学 女性の視点から見た現代社会
大羽 宏一 編（ Role： Joint author , 時代の変化と食生活の変化）
法律文化社 2007.10
Language：Japanese Book type：Textbook, survey, introduction
MISC 【 display / non-display 】
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養液栽培における根への環境ストレスの応用による野菜の高付加価値化
北野雅治, 日高功太, 圖師一文, 荒木卓哉
植物環境工学 20 210 - 218 2008.12
Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (scientific journal) Publisher：日本生物環境工学会
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高品質トマト生産のための塩利用
圖師一文
日本海水学会誌 62 179 - 186 2008.8
Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (scientific journal) Publisher：日本海水学会
Presentations 【 display / non-display 】
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長期栽培トマトにおけるテクスチャーおよびペクチン代謝の季節変動
圖師一文、西村圭、青木咲樹、山添瑚夏、高橋ひろみ
日本生物環境工学会2024年度九州支部会
Event date： 2024.12.8
Language：Japanese Presentation type：Oral presentation (general)
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塩ストレスがトマト果実のテクスチャーに与える影響の季節間差
宮﨑陽馬、黒木千夜子、小原颯一郎、佐藤美結、三原理子、圖師一文
日本生物環境工学会2024年度九州支部会
Event date： 2024.12.8
Language：Japanese Presentation type：Oral presentation (general)
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水分・塩ストレス処理とトマト栽培 Invited
圖師一文
日本砂丘学会第69回全国大会
Event date： 2024.9.26 - 2024.9.27
Language：Japanese Presentation type：Symposium, workshop panel (nominated)
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塩ストレスが長期栽培したトマトの貯蔵に伴う食味およびテクスチャーの変化に及ぼす影響とネットワーク分析による関連性評価
圖師一文, 黒田仁美, 山添瑚夏, 三原理子
日本生物環境工学会九州支部 2023 年北九州大会シンポジウム
Event date： 2023.12.16
Language：Japanese Presentation type：Poster presentation
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長期多段取り栽培がミニトマト果実の貯蔵中の食味，テクスチャーに及ぼす影響
三原理子, 圖師一文
日本生物環境工学会九州支部 2023 年北九州大会シンポジウム
Event date： 2023.12.16
Language：Japanese Presentation type：Poster presentation
Awards 【 display / non-display 】
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ベストプレゼンテーション賞
2024.12 日本生物環境工学会九州支部
宮﨑陽馬、黒木千夜子、小原颯一郎、佐藤美結、三原理子、圖師一文
Award type：Award from Japanese society, conference, symposium, etc.
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園芸学会九州支部賞学術賞
2019.8 園芸学会九州支部 塩ストレスを利用したトマトの高品質化に関する研究
圖師一文
Award type：Award from Japanese society, conference, symposium, etc. Country：Japan
Grant-in-Aid for Scientific Research 【 display / non-display 】
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栽培履歴が野菜貯蔵中の機能性成分含量に及ぼす影響と変動予測モデルの構築
Grant number：23K21249 2024.04 - 2025.03
独立行政法人日本学術振興会 科学研究費基金 基盤研究(B)
Authorship：Principal investigator
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栽培履歴が野菜貯蔵中の機能性成分含量に及ぼす影響と変動予測モデルの構築
Grant number：21H02319 2021.04 - 2025.03
独立行政法人日本学術振興会 科学研究費補助金 基盤研究(B)
Authorship：Principal investigator
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農業ビッグデータ活用によるトマト機能性成分の高含有化条件の探索と予測モデルの 構築
Grant number：18K05907 2018.04 - 2021.03
科学研究費補助金 基盤研究(C)
Authorship：Principal investigator
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良食味・高ポリフェノール含有トマト作出のための栽培環境と変動機構の解明
Grant number：15K07669 2015.04 - 2018.03
科学研究費補助金 基盤研究(C)
Authorship：Principal investigator
様々な栽培環境下におけるポリフェノール含量・組成の変化と変動メカニズムを明らかにするとともに，食味との関連性も調べ，良食味・高ポリフェノール含有トマトの作出を目指す.
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環境ストレス下のトマトにおける高品質安定生産に向けたネットワーク分析の応用
Grant number：24780245 2012.04 - 2015.03
科学研究費補助金 若手研究(B)
Authorship：Principal investigator
ネットワーク分析は，社会学や生物学等の分野において要因間の関連性を明らかにするために用いられている．本研究は，ネットワーク分析を用いた全く新しいアプローチにより，環境ストレス下におけるトマトの高品質安定生産に向けた環境調節技術の基盤技術を得ることを目的とする．具体的には，さまざまな環境ストレス下のトマトにおけるストレス指標や果実品質構成要素のネットワーク分析を行い，相互作用の可視化とそのメカニズムを明らかにするとともに，高品質安定生産のための品質予測技術を確立する．