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Faculty of Medicine School of Medicine Department of Medical Sciences, Structural Biology |
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WADA Kei
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Research Areas 【 display / non-display 】
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Life Science / Structural biochemistry
Papers 【 display / non-display 】
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Protonation/deprotonation-driven switch for the redox stability of low-potential [4Fe-4S] ferredoxin Reviewed
Kei Wada*, Kenji Kobayashi, Iori Era, Yusuke Isobe, Taigo Kamimura, Masaki MarukawaTakayuki NagaeKazuki HonjoNoriko KasedaYumiko MotoyamaKengo InoueMasakazu Sugishima, Katsuhiro, KusakaNaomine Yano, Keiichi Fukuyama, Masaki Mishima, Yasutaka Kitagawa, Masaki Unno
eLife 2024.12
Authorship：Lead author, Corresponding author Language：English Publishing type：Research paper (scientific journal)
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The minimal SUF system can substitute for the canonical iron-sulfur cluster biosynthesis systems by using inorganic sulfide as the sulfur source
Maya Murata, Taichi Murakami, Eiki Yuda, Nanami Mukai, Xintong Zheng, Natsumi Kurachi, Sachiko Mori, Shoko Ogawa, Kouhei Kunichika, Takashi Fujishiro, Kei Wada, Yasuhiro Takahashi
bioRxiv 2024.3
Language：English Publishing type：Research paper (scientific journal)
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Shinkawa N., Marukawa M., Wada K., Yukawa N.
Legal Medicine 64 2023.9
Language：English Publishing type：Research paper (scientific journal) Publisher：Legal Medicine
The Acetaminophen Detection Kit® (Kanto Chemical Company Co. Inc., Tokyo, Japan) is a colorimetric test based on an indophenol reaction. The test involves three reactions: deproteination of the sample, hydrolysis of acetaminophen to yield p-aminophenol, and coupling p-aminophenol with a derivative of phenol in alkali conditions to form a blue-colored indophenol dye. The kit was devised to accomplish these three reactions with only two reagents, allowing the prompt diagnosis of acetaminophen overdose in emergency medicine. In the user instructions included with the kit and in reports introducing the kit, the chemical composition of the two reagents was not disclosed. Details about the composition can be found in the Safety Data Sheet from the manufacturer; however, there is little explanation about the principle (mechanism) of the coupling reaction. This lack of information appears to have hampered the use of this kit in forensic medicine. In this report, we conducted the coupling reaction by successively adding the two reagents to a p-aminophenol (intermediate molecule) solution with the reaction vessel open to the air and under an anaerobic condition. Development of the blue color was inhibited in the absence of air but gradually developed when the reaction vessel was opened to air. Thus, the coupling reaction is an oxidation–reduction (redox) reaction that requires molecular oxygen (O2) dissolved from the air to act as an oxidant. This finding corroborates statements in previous reports and will hopefully facilitate the use of the kit for forensic purposes.
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Diversity in Infection Specificity between the Bloom-forming Microalga Heterosigma akashiwo and Its dsDNA Virus, Heterosigma akashiwo Virus Reviewed
Yusaku Funaoka, Haruna Hiromoto, Daichi Morimoto, Michiko Takahashi, Kei Wada, and Keizo Nagasaki
Microbes Environ. 38 ( 2 ) ME23036 - ME23036 2023.6
Language：English Publishing type：Research paper (scientific journal)
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Neutron crystallography and quantum chemical analysis of bilin reductase PcyA mutants reveal substrate and catalytic residue protonation states Reviewed International coauthorship
Tatsuya Joutsuka, Ryota Nanasawa, Keisuke Igarashi, Kazuki Horie, Masakazu Sugishima, Yoshinori Hagiwara, Kei Wada, Keiichi Fukuyama, Naomine Yano, Seiji Mori, Andreas Ostermann, Katsuhiro Kusaka, and Masaki Unno
J. Biol. Chem. 299 ( 1 ) 102763 2023.1
Language：English Publishing type：Research paper (scientific journal)
Books 【 display / non-display 】
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極限環境の生命化学
和田啓・鈴木秀之（ Role： Joint author , 塩耐性タンパク質）
日本化学会 2014.11
Language：Japanese Book type：Scholarly book
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Structure-function relationships of ferredoxin-dependent bilin reductases in "An integrating approach to photo functional hybrid materials for energy and environment"
Unno, M., Sugishima, M., Wada, K. and Fukuyama, K. （ Role： Joint author）
Nova Science Publishers 2013.6
Language：English Book type：Scholarly book
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γ-Glutamyltranspeptidase and Its Precursor in "Handbook of Proteolytic Enzymes 3"
Hiratake,J., Suzuki,H., Fukuyama,K.,Wada, K. and Kumagai H. （ Role： Joint author）
Academic Press 2012.12
Language：English Book type：Scholarly book
MISC 【 display / non-display 】
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基礎医学研究への蛋白質立体構造情報の活用 Invited
和田啓、元山祐美子
宮崎県医師会医学会誌 49 ( 1 ) 1 - 7 2025.4
Authorship：Lead author, Corresponding author Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (scientific journal)
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ビリン還元酵素PcyAの二つの変異体の吸収スペクトルおよび反応の変化とプロトン化状態との相関 Reviewed International coauthorship
海野昌喜 , 城塚達也 , 杉島正一 , 和田啓 , 萩原義徳 , 矢野直峰, Andreas Ostermann , 日下勝弘
波紋 (日本中性子科学会) 33 ( 4 ) 151 - 156 2023.11
Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (scientific journal)
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新刊紹介: 生体高分子結晶の X 線構造解析 実践手法と基礎理論 Invited Reviewed
和田 啓・福山 恵一
日本結晶学会誌 64 ( 2 ) 182 - 182 2022.5
Authorship：Lead author, Corresponding author Language：Japanese Publishing type：Book review, literature introduction, etc.
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Study of Structural Basis for Molecular Mechanisms of Cellular Redox Control Reviewed
63 ( 2 ) 105 - 112 2021.5
Authorship：Lead author, Last author, Corresponding author Publishing type：Article, review, commentary, editorial, etc. (scientific journal)
DOI： 10.5940/jcrsj.63.105
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グルタチオンを分解する酵素の立体構造とその産業応用 Invited
和田啓、渡辺文太、鈴木秀之、福山恵一
バイオサイエンスとバイオインダストリー 78 ( 3 ) 209 - 212 2020.5
Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (scientific journal)
Presentations 【 display / non-display 】
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クライオ電顕単粒子解析により明らかになった鉄-硫黄クラスター生合成タンパク質SufBCDの動的挙動
辻実怜、綛田紀子、元山祐美子、内田丈聖、横山武司、和田啓、田中良和
2024年度生物物理学会北海道支部－東北支部合同例会 2025.2.21
Event date： 2025.2.21
Language：Japanese Presentation type：Oral presentation (general)
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窒素固定シアノバクテリアLeptolyngbya boryanaにおけるCnfRによるnif遺伝子の転写活性化に対するNtcAの関与 International coauthorship
秦武志、馬場真里、上坂一馬、安達円香、Tsend-Ayush Badbold、杉原初奈、辻本良真、元山祐美子、和田啓、山本治樹、藤田祐一
藍藻の分子生物学2024 2024.12.13
Event date： 2024.12.13 - 2024.12.14
Language：Japanese Presentation type：Poster presentation
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「超硫黄分子が関与するFe-Sクラスター生合成複合体の構造機能解析」 Invited
和田 啓
名古屋大学大学院生命農学研究科 ゲノム情報機能学セミナー 2024.11.11
Event date： 2024.11.11
Language：Japanese Presentation type：Public lecture, seminar, tutorial, course, or other speech
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X-ray and Cryo-EM structures of the SUF system involved in de novo iron-sulfur cluster biosynthesis Invited International coauthorship
Kei Wada
第97回日本生化学会大会 2024.11.8
Event date： 2024.11.6 - 2024.11.8
Language：English Presentation type：Symposium, workshop panel (nominated)
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アセトアミノフェン検出反応における分子状酸素の関与
丸川正貴、新川慶明、綛田紀子、元山祐美子、柿崎英二、園田愛、 湯川修弘、和田啓
生物機能研究会2024 2024.7.13
Event date： 2024.7.13
Language：Japanese Presentation type：Poster presentation
Awards 【 display / non-display 】
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日本結晶学会 学術賞
2020.11 日本結晶学会
和田啓
Award type：Award from Japanese society, conference, symposium, etc. Country：Japan
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日本結晶学会進歩賞
2009.12 日本結晶学会
和田啓
Award type：International academic award (Japan or overseas) Country：Japan
Grant-in-Aid for Scientific Research 【 display / non-display 】
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タンパク質構造生物化学に立脚した超硫黄分子の可視化
Grant number：21H05260 2021.10 - 2026.03
独立行政法人日本学術振興会 科学研究費補助金 学術変革領域研究(A) [計画研究代表]
Authorship：Principal investigator
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正２０面体球状ウイルス粒子の階層的な形成機序とpH依存的崩壊の制御
Grant number：23K17981 2023.04 - 2026.03
独立行政法人日本学術振興会 科学研究費基金 挑戦的研究(萌芽)
Authorship：Principal investigator
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ウイルスによる赤潮崩壊現象を裏打ちする分子生態学的メカニズムに関する研究
Grant number：22H00385 2022.04 - 2027.03
独立行政法人日本学術振興会 科学研究費補助金 基盤研究(A)
Authorship：Coinvestigator(s)
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組織レニン・アンジオテンシン系におけるビッグアンジオテンシー25の意義
Grant number：22K08188 2022.04 - 2025.03
独立行政法人日本学術振興会 科学研究費基金 基盤研究(C)
Authorship：Coinvestigator(s)
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生体必須の金属補因子「鉄硫黄クラスター」の生合成機序と無細胞構築系の確立
Grant number：20H03196 2020.04 - 2024.03
独立行政法人日本学術振興会 科学研究費補助金 基盤研究(B)
Authorship：Principal investigator
Available Technology 【 display / non-display 】
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タンパク質構造解析を利用した創薬基盤の確立
タンパク質の原子構造に基づく作動機構の解明
酸素に脆弱な生体補因子の同定および構造決定Home Page： 医学部 機能制御学講座 蛋白質機能学分野
Related fields where technical consultation is available：・タンパク質の立体構造解析（X線結晶構造解析、結晶化スクリーニング）
・タンパク質の大量発現・精製Message：・共同研究の希望テーマ：タンパク質の構造解析