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Faculty of Medicine School of Medicine Department of Medical Sciences, Structural Biology |
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Professor |
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Related SDGs |
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WADA Kei
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Research Areas 【 display / non-display 】
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Life Science / Structural biochemistry
Papers 【 display / non-display 】
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Single Hydrogen Atom Controls Redox Behavior of Iron–Sulfur Clusters in Ferredoxin Invited Reviewed
M. Unno, K. Kobayashi, K. Kusaka, N. Yano, Y. Kitagawa and K. Wada
MLF Annual Report 2024 Research and Development Highlights 2024 1 - 2 2025.12
Authorship：Last author, Corresponding author Language：English Publishing type：Research paper (bulletin of university, research institution)
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Combining X-Ray and Neutron Crystallography with in Silico DFT Analyses of Low-Potential [4Fe-4S] Ferredoxin Invited Reviewed
M. Unno, Y. Kitagawa and K. Wada
Highlight Photon Factoty 2025 ( 1 ) 30 - 31 2025.8
Authorship：Corresponding author Language：English Publishing type：Research paper (bulletin of university, research institution)
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Protonation/deprotonation-driven switch for the redox stability of low-potential [4Fe-4S] ferredoxin Reviewed
Kei Wada*, Kenji Kobayashi, Iori Era, Yusuke Isobe, Taigo Kamimura, Masaki Marukawa , Takayuki Nagae , Kazuki Honjo , Noriko Kaseda , Yumiko Motoyama , Kengo Inoue, Masakazu Sugishima, Katsuhiro Kusaka , Naomine Yano, Keiichi Fukuyama, Masaki Mishima, Yasutaka Kitagawa, Masaki Unno
eLife 2024.12
Authorship：Lead author, Corresponding author Language：English Publishing type：Research paper (scientific journal)
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The minimal SUF system can substitute for the canonical iron-sulfur cluster biosynthesis systems by using inorganic sulfide as the sulfur source
Maya Murata, Taichi Murakami, Eiki Yuda, Nanami Mukai, Xintong Zheng, Natsumi Kurachi, Sachiko Mori, Shoko Ogawa, Kouhei Kunichika, Takashi Fujishiro, Kei Wada, Yasuhiro Takahashi
bioRxiv 2024.3
Language：English Publishing type：Research paper (scientific journal)
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Shinkawa N., Marukawa M., Wada K., Yukawa N.
Legal Medicine 64 2023.9
Language：English Publishing type：Research paper (scientific journal) Publisher：Legal Medicine
The Acetaminophen Detection Kit® (Kanto Chemical Company Co. Inc., Tokyo, Japan) is a colorimetric test based on an indophenol reaction. The test involves three reactions: deproteination of the sample, hydrolysis of acetaminophen to yield p-aminophenol, and coupling p-aminophenol with a derivative of phenol in alkali conditions to form a blue-colored indophenol dye. The kit was devised to accomplish these three reactions with only two reagents, allowing the prompt diagnosis of acetaminophen overdose in emergency medicine. In the user instructions included with the kit and in reports introducing the kit, the chemical composition of the two reagents was not disclosed. Details about the composition can be found in the Safety Data Sheet from the manufacturer; however, there is little explanation about the principle (mechanism) of the coupling reaction. This lack of information appears to have hampered the use of this kit in forensic medicine. In this report, we conducted the coupling reaction by successively adding the two reagents to a p-aminophenol (intermediate molecule) solution with the reaction vessel open to the air and under an anaerobic condition. Development of the blue color was inhibited in the absence of air but gradually developed when the reaction vessel was opened to air. Thus, the coupling reaction is an oxidation–reduction (redox) reaction that requires molecular oxygen (O2) dissolved from the air to act as an oxidant. This finding corroborates statements in previous reports and will hopefully facilitate the use of the kit for forensic purposes.
Books 【 display / non-display 】
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Iron in Biology: Molecular Structures, Cellular Processes and Living Systems
M. Sugishima; K. Wada; J. Taira（ Role： Joint author , Chapter 9: Proteins Associated with Haem Catabolism for Iron Utilisation and Other Functions in Mammals and Photosynthetic Organisms）
2025.6
Language：English Book type：Scholarly book
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極限環境の生命化学
和田啓・鈴木秀之（ Role： Joint author , 塩耐性タンパク質）
日本化学会 2014.11
Language：Japanese Book type：Scholarly book
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Structure-function relationships of ferredoxin-dependent bilin reductases in "An integrating approach to photo functional hybrid materials for energy and environment"
Unno, M., Sugishima, M., Wada, K. and Fukuyama, K. （ Role： Joint author）
Nova Science Publishers 2013.6
Language：English Book type：Scholarly book
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γ-Glutamyltranspeptidase and Its Precursor in "Handbook of Proteolytic Enzymes 3"
Hiratake,J., Suzuki,H., Fukuyama,K.,Wada, K. and Kumagai H. （ Role： Joint author）
Academic Press 2012.12
Language：English Book type：Scholarly book
MISC 【 display / non-display 】
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基礎医学研究への蛋白質立体構造情報の活用 Invited
和田啓、元山祐美子
宮崎県医師会医学会誌 49 ( 1 ) 1 - 7 2025.4
Authorship：Lead author, Corresponding author Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (scientific journal)
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ビリン還元酵素PcyAの二つの変異体の吸収スペクトルおよび反応の変化とプロトン化状態との相関 Reviewed International coauthorship
海野昌喜 , 城塚達也 , 杉島正一 , 和田啓 , 萩原義徳 , 矢野直峰, Andreas Ostermann , 日下勝弘
波紋 (日本中性子科学会) 33 ( 4 ) 151 - 156 2023.11
Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (scientific journal)
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新刊紹介: 生体高分子結晶の X 線構造解析 実践手法と基礎理論 Invited Reviewed
和田 啓・福山 恵一
日本結晶学会誌 64 ( 2 ) 182 - 182 2022.5
Authorship：Lead author, Corresponding author Language：Japanese Publishing type：Book review, literature introduction, etc.
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Study of Structural Basis for Molecular Mechanisms of Cellular Redox Control Reviewed
63 ( 2 ) 105 - 112 2021.5
Authorship：Lead author, Last author, Corresponding author Publishing type：Article, review, commentary, editorial, etc. (scientific journal)
DOI： 10.5940/jcrsj.63.105
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グルタチオンを分解する酵素の立体構造とその産業応用 Invited
和田啓、渡辺文太、鈴木秀之、福山恵一
バイオサイエンスとバイオインダストリー 78 ( 3 ) 209 - 212 2020.5
Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (scientific journal)
Presentations 【 display / non-display 】
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Structural and functional analyses of iron-sulfur cluster biogenesis involving supersulfide Invited
2026.3.11
Event date： 2026.3.10 - 2026.3.12
Language：Japanese Presentation type：Oral presentation (invited, special)
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鉄硫黄クラスター生合成を担うSufBC複合体の超硫黄分子認識の構造基盤
内田丈聖、辻実怜、元山祐美子、綛田紀子、横山武司、田中良和、和田啓
第98回日本生化学会 2025.11.4
Event date： 2025.11.3 - 2025.11.5
Language：Japanese Presentation type：Oral presentation (general)
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Mechanism of Redox Potential Control in the Electron Transfer Protein Ferredoxin Revealed Using Combined X-ray and Neutron Crystallography
2025.10.30
Event date： 2025.10.30 - 2025.10.31
Language：Japanese Presentation type：Oral presentation (general)
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Cryo-EM structure of SufBC2D, Fe-S cluster biosynthesis scaffold protein International conference
Misato Tsuji, Josei Uchida, Yumiko Motoyama, Takeshi Yokoyama, Kei Wada, Yoshikazu Tanaka
Tohoku Univ. and Case Western Reserve University Symposium 2025.8.5
Event date： 2025.8.5
Language：English Presentation type：Poster presentation
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森林落葉によるルミノール発光の原因因子の探索
元山祐美子、橘未羽、内田丈聖、野間貫太、丸川正貴、水元幹太、綛田紀子、新川慶明、柿崎英二、園田愛、湯川修弘、和田啓
第25回日本蛋白質科学会年会 2025.6.18
Event date： 2025.6.18 - 2025.6.20
Presentation type：Poster presentation
Awards 【 display / non-display 】
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日本結晶学会 学術賞
2020.11 日本結晶学会
和田啓
Award type：Award from Japanese society, conference, symposium, etc. Country：Japan
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日本結晶学会進歩賞
2009.12 日本結晶学会
和田啓
Award type：International academic award (Japan or overseas) Country：Japan
Grant-in-Aid for Scientific Research 【 display / non-display 】
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タンパク質構造生物化学に立脚した超硫黄分子の可視化
Grant number：21H05260 2021.10 - 2026.03
独立行政法人日本学術振興会 科学研究費補助金 学術変革領域研究(A) [計画研究代表]
Authorship：Principal investigator
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高効率細胞外電子伝達の最小単位
Grant number：25K08883 2025.04 - 2028.03
独立行政法人日本学術振興会 科学研究費基金 基盤研究(C)
Authorship：Coinvestigator(s)
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高効率細胞外電子伝達の最小単位
Grant number：25K08883 2025.04 - 2028.03
基盤研究(C)
Authorship：Coinvestigator(s)
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正２０面体球状ウイルス粒子の階層的な形成機序とpH依存的崩壊の制御
Grant number：23K17981 2023.04 - 2026.03
独立行政法人日本学術振興会 科学研究費基金 挑戦的研究(萌芽)
Authorship：Principal investigator
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正２０面体球状ウイルス粒子の階層的な形成機序とpH依存的崩壊の制御
Grant number：23K17981 2023.04 - 2026.03
挑戦的研究（萌芽）
Authorship：Principal investigator
Available Technology 【 display / non-display 】
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タンパク質構造解析を利用した創薬基盤の確立
タンパク質の原子構造に基づく作動機構の解明
酸素に脆弱な生体補因子の同定および構造決定Home Page： 医学部 機能制御学講座 蛋白質機能学分野
Related fields where technical consultation is available：・タンパク質の立体構造解析（X線結晶構造解析、結晶化スクリーニング）
・タンパク質の大量発現・精製Message：・共同研究の希望テーマ：タンパク質の構造解析