論文 - 和田 啓
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Protonation/deprotonation-driven switch for the redox stability of low-potential [4Fe-4S] ferredoxin 査読あり
Kei Wada*, Kenji Kobayashi, Iori Era, Yusuke Isobe, Taigo Kamimura, Masaki MarukawaTakayuki NagaeKazuki HonjoNoriko KasedaYumiko MotoyamaKengo InoueMasakazu Sugishima, Katsuhiro, KusakaNaomine Yano, Keiichi Fukuyama, Masaki Mishima, Yasutaka Kitagawa, Masaki Unno
eLife 2024年12月
担当区分：筆頭著者, 責任著者 記述言語：英語 掲載種別：研究論文（学術雑誌）
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The minimal SUF system can substitute for the canonical iron-sulfur cluster biosynthesis systems by using inorganic sulfide as the sulfur source
Maya Murata, Taichi Murakami, Eiki Yuda, Nanami Mukai, Xintong Zheng, Natsumi Kurachi, Sachiko Mori, Shoko Ogawa, Kouhei Kunichika, Takashi Fujishiro, Kei Wada, Yasuhiro Takahashi
bioRxiv 2024年3月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Shinkawa N., Marukawa M., Wada K., Yukawa N.
Legal Medicine 64 2023年9月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Legal Medicine
The Acetaminophen Detection Kit® (Kanto Chemical Company Co. Inc., Tokyo, Japan) is a colorimetric test based on an indophenol reaction. The test involves three reactions: deproteination of the sample, hydrolysis of acetaminophen to yield p-aminophenol, and coupling p-aminophenol with a derivative of phenol in alkali conditions to form a blue-colored indophenol dye. The kit was devised to accomplish these three reactions with only two reagents, allowing the prompt diagnosis of acetaminophen overdose in emergency medicine. In the user instructions included with the kit and in reports introducing the kit, the chemical composition of the two reagents was not disclosed. Details about the composition can be found in the Safety Data Sheet from the manufacturer; however, there is little explanation about the principle (mechanism) of the coupling reaction. This lack of information appears to have hampered the use of this kit in forensic medicine. In this report, we conducted the coupling reaction by successively adding the two reagents to a p-aminophenol (intermediate molecule) solution with the reaction vessel open to the air and under an anaerobic condition. Development of the blue color was inhibited in the absence of air but gradually developed when the reaction vessel was opened to air. Thus, the coupling reaction is an oxidation–reduction (redox) reaction that requires molecular oxygen (O2) dissolved from the air to act as an oxidant. This finding corroborates statements in previous reports and will hopefully facilitate the use of the kit for forensic purposes.
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Diversity in Infection Specificity between the Bloom-forming Microalga Heterosigma akashiwo and Its dsDNA Virus, Heterosigma akashiwo Virus 査読あり
Yusaku Funaoka, Haruna Hiromoto, Daichi Morimoto, Michiko Takahashi, Kei Wada, and Keizo Nagasaki
Microbes Environ. 38 ( 2 ) ME23036 - ME23036 2023年6月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Neutron crystallography and quantum chemical analysis of bilin reductase PcyA mutants reveal substrate and catalytic residue protonation states 査読あり 国際共著
Tatsuya Joutsuka, Ryota Nanasawa, Keisuke Igarashi, Kazuki Horie, Masakazu Sugishima, Yoshinori Hagiwara, Kei Wada, Keiichi Fukuyama, Naomine Yano, Seiji Mori, Andreas Ostermann, Katsuhiro Kusaka, and Masaki Unno
J. Biol. Chem. 299 ( 1 ) 102763 2023年1月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Degenerate PCR Targeting the Major Capsid Protein Gene of HcRNAV and Related Viruses 査読あり
Michiko Takahashi, Kei Wada, Syun-ichi Urayama, Yuichi Masuda, Keizo Nagasaki
Microbes and Environments 37 ( 5 ) ME21075 - ME21075 2022年4月
担当区分：筆頭著者 記述言語：英語 掲載種別：研究論文（学術雑誌）
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Domain movements of NADPH–cytochrome P450 oxidoreductase (CPR) are required for the smooth electron transfer from CPR to heme–heme oxygenase-1 (HO-1) complex 査読あり
M. Sugishima, J. Taira, M. Iijima, H. Sato, K. Wada, M. Noguchi, K. Fukuyama, M. Takano, H. Sakamoto and K. Yamamoto
Acta Crystallographica Section A: Foundations and Advances A77 C1130 - C1130 2021年8月
記述言語：英語 掲載種別：研究論文（国際会議プロシーディングス）
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Structural basis of the protochromic green/red photocycle of the chromatic acclimation sensor RcaE 査読あり
Takayuki Nagae, Masashi Unno, Taiki Koizumi, Yohei Miyanoiri, Tomotsumi Fujisawa, Kento Masui, Takanari Kamo, Kei Wada, Toshihiko Eki, Yutaka Itoc, Yuu Hirosee,, and Masaki Mishima
Proceedings of the National Academy of Sciences of the United States of America ( 118 ) 2021年5月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Crystal structures of hydroxymethylbilane synthase complexed with a substrate analog: a single substrate-binding site for four consecutive condensation step 査読あり
Sato H., Sugishima M., Tsukaguchi M., Masuko T., Iijima M., Takano M., Omata Y., Hirabayashi K., Wada K., Hisaeda Y., Yamamoto K.
Biochem. J. 478 1023 - 1042 2021年3月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Pathological Features of Ruptured Coronary Plaque and Thrombus Interfaces: Fibrin and von Willebrand Factor as Platelet Scaffolds on Rupture Sites 査読あり
Atsushi Yamashita, Kensaku Nishihira, Toshihiro Gi, Kazunari Maekawa, Kinta Hatakeyama, Saki Horiuchi, Kei Wada, Yoshisato Shibata, Yujiro Asada
Thromb Haemost 121 ( 2 ) 234 - 241 2021年2月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Chronological distribution of dinoflagellate-infecting RNA virus in marine sediment core 査読あり
MichikoTakahashi, Kei Wada, Yoshihito Takano, Kyouhei Matsuno, Yuichi Masuda, Kazuno Arai, Masafumi Murayama, Yuji Tomaru, Kouki Tanaka, Keizo Nagasaki
Science of The Total Environment ( 770 ) 145220 - 145228 2021年1月
担当区分：筆頭著者 記述言語：英語 掲載種別：研究論文（学術雑誌）
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Evidence for dynamic in vivo interconversion of the conformational states of IscU during iron‐sulfur cluster biosynthesis 査読あり
Sakiko Sato Yumeka Matsushima Miaki Kanazawa Naoyuki Tanaka Takashi Fujishiro Kouhei Kunichika Ryosuke Nakamura Hiroaki Tomioka Kei Wada Yasuhiro Takahashi
Molecular Microbiology 1 - 12 2020年11月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Crystal structure of phytochromobilin synthase in complex with biliverdin IXα, a key enzyme in the biosynthesis of phytochrome 査読あり
Masakzu Sugishima, Kei Wada, Keiichi Fukuyama and Ken Yamamoto
Journal of Biological Chemistry 3 ( 295 ) 771 - 782 2020年1月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Bilin-metabolizing enzymes: site-specific reductions catalyzed by two different type of enzymes 招待あり 査読あり
Masakazu Sugishima, KeiWada, Masaki Unno, Keiichi Fukuyama
Current Opinion in Structural Biology ( 59 ) 73 - 80 2019年12月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Super-activator variants of the cyanobacterial transcriptional regulator ChlR essential for tetrapyrrole biosynthesis under low oxygen conditions 査読あり
Yuto Hiraide, Haruki Yamamoto, Yasushi Kawajiri, Hisanori Yamakawa, Kei Wada & Yuichi Fujita
Bioscience, Biotechnology, and Biochemistry ( 84 ) 481 - 490 2019年11月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Identification of IscU residues critical for de novo iron‐sulfur cluster assembly 査読あり
Naoyuki Tanaka, Eiki Yuda, Takashi Fujishiro, Kei Hirabayashi, Kei Wada, Yasuhiro Takahashi
Molecular Microbiology 6 ( 112 ) 2019年9月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Crystal structure of a NADPH‐cytochrome P450 oxidoreductase (CYPOR) and heme oxygenase 1 fusion protein implies a conformational change in CYPOR upon NADPH/NADP+ binding 査読あり
Masakazu Sugishima, Hideki Sato, Kei Wada, Kei Wada, Ken Yamamoto
FEBS Letters 2019年3月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Recent Advances in the Understanding of the Reaction Chemistries of the Heme Catabolizing Enzymes HO and BVR Based on High Resolution Protein Structures 査読あり
Sugishima M, Wada K, Fukuyama K.
Current Medicinal Chemistry 2018年12月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Crystal Growth of a Bilin Reductase PcyA I86D Mutant-Substrate Complex for Neutron Crystallography 査読あり
Keisuke Igarashi, Yoshinori Hagiwara, Masakazu Sugishima, Kei Wada, Keiichi Fukuyama, Atsushi Ikeda,Naomine Yano, Katsuhiro Kusaka, Andreas Ostermann, Masaki Unno
Crystal Growth & Design. 18 ( 9 ) 5181 - 5181 2018年7月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Synthesis and Evaluation of the Inhibitory Activity of the Four Stereoisomers of the Potent and Selective Human γ-Glutamyl Transpeptidase Inhibitor GGsTop 査読あり
Bunta Watanabe, Yukiko Tabucchi, Kei Wada, Jun Hiratake
Bioorganic & Medicinal Chemistry Letters 24 ( 21 ) 5340 - 5352 2017年11月
記述言語：英語 掲載種別：研究論文（学術雑誌）