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Affiliation |
Faculty of Regional Innovation |
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Title |
Associate Professor |
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Contact information |
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External Link |
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HASHIGUCHI Masatsugu
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Degree 【 display / non-display 】
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博士（農学） ( 2007.3 鹿児島大学 )
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修士（農学） ( 2001.3 宮崎大学 )
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学士（農学） ( 1999.3 宮崎大学 )
Research Areas 【 display / non-display 】
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Environmental Science/Agriculture Science / Crop production science / 栽培学
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Environmental Science/Agriculture Science / Science in plant genetics and breeding / 生物遺伝資源
Education 【 display / non-display 】
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Kagoshima University
2001.4 - 2004.3
Country：Japan
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University of Miyazaki Graduate School, Division of Agriculture
1999.4 - 2001.3
Country：Japan
Campus Career 【 display / non-display 】
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University of Miyazaki Faculty of Regional Innovation Associate Professor
2021.10 - Now
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University of Miyazaki Faculty of Agriculture Department of Animal and Grassland Sciences Assistant Professor
2013.04 - 2021.09
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University of Miyazaki Frontier Science Research Center Assistant Professor
2012.05 - 2013.03
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University of Miyazaki Frontier Science Research Center Part-time researcher for university or other academic organization
2006.04 - 2012.03
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University of Miyazaki Faculty of Agriculture Department of Biological Production and Environmental Sciences Meadow environmental science course Part-time researcher for university or other academic organization
2004.04 - 2006.03
Papers 【 display / non-display 】
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Assessing genetic diversity and geographical differentiation in a global collection of wild soybean (Glycine soja Sieb. et Zucc.) and assigning a mini-core collection. Reviewed
Li F, Sayama T, Yokota Y, Hiraga S, Hashiguchi M, Tanaka H, Akashi R, Ishimoto M
DNA research : an international journal for rapid publication of reports on genes and genomes 2024.3
Language：English Publishing type：Research paper (scientific journal)
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Development of a digital phenotyping system using 3D model reconstruction for zoysiagrass Reviewed
Pongpiyapaiboon S., Tanaka H., Hashiguchi M., Hashiguchi T., Hayashi A., Tanabata T., Isobe S., Akashi R.
Plant Phenome Journal 6 ( 1 ) 2023.12
Language：English Publishing type：Research paper (scientific journal) Publisher：Plant Phenome Journal
Digital phenotyping, particularly the use of plant 3D models, is a promising method for high-throughput plant evaluation. Although many recent studies on the topic have been published, further research is needed to apply it to breeding research and other related fields. In this study, using a 3D model phenotyping system we developed, we reconstructed and analyzed 20 accessions of zoysiagrass (Zoysia spp.), including three species and their hybrid, over a period of 1 year. Artificial neural network with three hidden layers was able to effectively remove nonplant parts while retaining plant parts that were incorrectly removed using the cropping method, offering a robust and flexible approach for post-processing of 3D models. The system also demonstrated its ability to accurately evaluate a range of traits, including height, area, and color using red green blue (RGB)-based vegetation indices. The results showed a high correlation between the estimated volume obtained from voxel 3D model and dry weight, enabling its use as a non-destructive method for measuring plant volume. In addition, we found that the green red normalized difference index from RGB-based indices was similar to the commonly used normalized difference vegetation index in controlled illumination conditions. These results demonstrate the potential for three-dimensional model phenotyping to facilitate plant breeding, particularly in the field of turfgrass and feed crops.
DOI： 10.1002/ppj2.20076
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Ng H.M., Gondo T., Ushiyama M., Cho S., Maemura S., Hashiguchi M., Tanaka H., Akashi R.
Grassland Science 69 ( 2 ) 152 - 161 2023.4
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Grassland Science
Zoysia matrella (L.) Merrill is a perennial C4 warm-season turfgrass grown for landscapes, golf courses, sports fields and recreation parks. To create a new breeding method by genome editing, an efficient genetic transformation system is essential. In this study, we report the efficient protocol of Agrobacterium-mediated transformation through the establishment of a stable tissue culture system for Z. matrella ‘Wakaba’. The embryogenic callus was induced from shoot apices of nodal segments incubated in Murashige and Skoog (MS) medium containing 2 mg/l 2,4-dichlorophnoxyacetic acid (2,4-D), 0.1 mg/l 6-benzylaminopurine (BAP) and 5 μM CuSO4 (MS-DBC). Repeated subculture of compact high-quality callus in MS-DBC medium produced a highly regenerative callus with dense pre-embryogenic clusters, and it was used as a transformation target. Agrobacterium strain EHA105 harboring pANIC8B vector containing the β-glucuronidase gene (GUS) and hygromycin B phosphotransferase gene was used. Agrobacterium-infected calli were cocultured for 5 days with 100 μM acetosyringone and then subjected to selection pressure of 50 mg/l hygromycin. This optimized protocol yielded transformation efficiencies of up to 6.6%. Southern blot analysis verified one to three copies of the GUS gene in different independent transgenic plants. All transgenic plants were morphologically normal, and the GUS expressions were stable. Our optimized in vitro and transgenic system will facilitate the new breeding technology of genome editing in zoysiagrass.
DOI： 10.1111/grs.12396
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異分野連携研究と地域を総合的に研究する試み―世界農業遺産（GIAHS）高千穂郷・椎葉山地域研究会の取組を事例として
井上果子・ 西和盛・ 松岡崇暢・ 鈴木良幸・橋口正嗣
宮崎大学地域資源創成学部研究紀要 ( 6 ) 9 - 15 2023.3
Publishing type：Research paper (bulletin of university, research institution)
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Oota M., Toyoda S., Kotake T., Wada N., Hashiguchi M., Akashi R., Ishikawa H., Favery B., Tsai A.Y.L., Sawa S.
Frontiers in Plant Science 13 2023.1
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Frontiers in Plant Science
Introduction: The soil houses a tremendous amount of micro-organisms, many of which are plant parasites and pathogens by feeding off plant roots for sustenance. Such root pathogens and parasites often rely on plant-secreted signaling molecules in the rhizosphere as host guidance cues. Here we describe the isolation and characterization of a chemoattractant of plant-parasitic root-knot nematodes (Meloidogyne incognita, RKN). Methods: The Super-growing Root (SR) culture, consisting of excised roots from the legume species Lotus corniculatus L., was found to strongly attract infective RKN juveniles and actively secrete chemoattractants into the liquid culture media. The chemo-attractant in the culture media supernatant was purified using hydrophobicity and anion exchange chromatography, and found to be enriched in carbohydrates. Results: Monosaccharide analyses suggest the chemo-attractant contains a wide array of sugars, but is enriched in arabinose, galactose and galacturonic acid. This purified chemoattractant was shown to contain pectin, specifically anti-rhamnogalacturonan-I and anti-arabinogalactan protein epitopes but not anti-homogalacturonan epitopes. More importantly, the arabinose and galactose sidechain groups were found to be essential for RKN-attracting activities. This chemo-attractant appears to be specific to M. incognita, as it wasn’t effective in attracting other Meloidogyne species nor Caenorhabditis elegans. Discussion: This is the first report to identify the nematode attractant purified from root exudate of L corniculatus L. Our findings re-enforce pectic carbohydrates as important chemicals mediating micro-organism chemotaxis in the soil, and also highlight the unexpected utilities of the SR culture system in root pathogen research.
Books 【 display / non-display 】
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The National BioRecource Project in Japan. In: Tabata S and Stugaard J(Eds) The Lotus japonicus genome
1. Hashiguchi M and Akashi R（ Role： Contributor）
Springer Berlin 2014.10
Language：English Book type：Scholarly book
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形質転換プロトコール植物編 田部井豊 編 2章 牧草2-5バーズフットトレフォイルの形質転換プロトコール
田中秀典・橋口正嗣・明石 良（ Role： Contributor）
化学同人 2012.9
Language：Japanese Book type：Scholarly book
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ミヤコグサ・ダイズ モデル植物から作物への展開
橋口正嗣、権藤崇裕、明石 良（ Role： Joint author , 橋口正嗣、権藤崇裕、明石 良）
秀潤社 2009.9
Language：Japanese Book type：Scholarly book
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Super roots in Lotus corniculatus: A unique tissue culture and regeneration system in a legume species) International journal
Akashi,R., Kawano,T., Hashiguchi,M., Kutsuna,Y., Hoffmann-Tsay,S.-S., Hoffmann,F.（ Role： Sole author）
Springer 2003.1
Language：English Book type：Scholarly book
MISC 【 display / non-display 】
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野生ダイズのゲノム情報および特性解析からみえてきた 遺伝的多様性とダイズのスマート育種への活用に向けて Invited
橋口 拓勇, 佐藤 修正, 田中 秀典, 橋口 正嗣
JATAFFジャーナル 2022.12
Authorship：Corresponding author Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (scientific journal)
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NBRPミヤコグサ・ダイズにおけるバイオリソース整備 Invited
橋口 正嗣，田中 秀典，橋口 拓勇，明石 良
農業および園芸 96 ( 2 ) 153 - 162 2021.2
Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (scientific journal)
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マメ科植物研究のためのミヤコグサバイオリソース Invited Reviewed
橋口正嗣，佐藤修正，橋口拓勇，田中秀典，明石 良
植物科学の最前線（BSJ-Review） 10 179 - 189 2019.7
Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (scientific journal) Publisher：日本植物学会
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Perspectives on the use of bioresources in breeding sciences: Lessons from successful studies Invited
Nasuda Shuhei, Ito Emi, Sato Yutaka, Hisano Hiroshi, Sato Kazuhiro, Komatsuda Takao, Ishikawa Ryo, Hashiguchi Masatsugu, Suzuki Akihiro, Hoshikawa Ken
Breeding Research 21 ( 1 ) 81 - 85 2019.6
Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (scientific journal) Publisher：Japanese Society of Breeding
DOI： 10.1270/jsbbr.21.W06
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大学における遺伝資源保存と教育（特集：最近の農業生物資源ジーンバンク事業の活動と遺伝資源の利用） Invited
田中秀典・橋口正嗣・明石 良
JATAFFジャーナル 2016.5
Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (bulletin of university, research institution)
Presentations 【 display / non-display 】
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RNAseq and physiological analysis in wild soybean lines with different low phosphorus tolerance
Kiriyama Yuto, Arun Daspute Abhijit, Ohmori Yoshihiro, Kanai Go, Obayashi Kan, Li Feng, Ishimoto Masao, Hashiguchi Masatsugu, Takano Junpei, Yanase Masanori
Abstracts of Meeting of the CSSJ 2024.3.28 CROP SCIENCE SOCIETY OF JAPAN
Event date： 2024.3.28 - 2024.3.29
Language：Japanese Presentation type：Poster presentation
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Genetic Analysis of Phosphorus Utilization Efficiency Using Wild Soybean Core Collection
Obayashi Kan, Kanai Go, Li Feng, Ishimoto Masao, Hashiguchi Masatsugu, Takano Junpei, Yanase Masanori
Abstracts of Meeting of the CSSJ 2024.3.28 CROP SCIENCE SOCIETY OF JAPAN
Event date： 2024.3.28 - 2024.3.29
Language：Japanese Presentation type：Poster presentation
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塩ストレス条件下のシバ属における被度変化
山本昭洋，齊藤七海，藤康樹，橋口正嗣，佐伯雄一，明石 良
第65回日本植物生理学会年会 2024.3.17
Event date： 2024.3.17 - 2024.3.19
Presentation type：Poster presentation
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Updated information on legume experimental resources in NBRP Lotus/Glycine International coauthorship International conference
Shusei Sato, Shun Hashimoto, Masaru Bamba, Yusdar Mustamin, Turgut Akyol, Stig Andersen, Takuyu Hashiguchi, Masatsugu Hashiguchi
The 6th Asian-Pacific Conference on Plant-Microbe Symbiosis and Nitrogen Fixation
Event date： 2024.1.7 - 2024.1.9
Presentation type：Oral presentation (invited, special)
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The National BioResource Project (NBRP) for Lotus and Glycine in Japan: QTL analysis for seed storability in soybean under different temperature International conference
Pakanan Jiamtae, Takuyu Hashiguchi, Shusei Sato, Hidenori Tanaka, Masatsugu Hashiguchi
The 6th Asian-Pacific Conference on Plant-Microbe Symbiosis and Nitrogen Fixation
Event date： 2024.1.7 - 2024.1.9
Presentation type：Poster presentation
Grant-in-Aid for Scientific Research 【 display / non-display 】
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植物ー微生物間の情報伝達分子としてのフラボノイド硫酸体に関する研究
Grant number：23K05027 2023 - 2026.03
独立行政法人日本学術振興会 科学研究費基金 基盤研究（C）
橋口 拓勇
Authorship：Coinvestigator(s)