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Affiliation |
Faculty of Agriculture Department of Biochemistry and Applied Biosciences |
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Title |
Professor |
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External Link |
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HIKIMA Junichi
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Research Areas 【 display / non-display 】
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Life Science / Aquatic bioproduction science
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Life Science / Aquatic life science
Papers 【 display / non-display 】
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Comprehensive analysis of diel rhythmic expression of the medaka toll-like receptor gene family Reviewed
Hata T., Shimawaki H., Setoguchi S., Morimoto N., Hikima J., Sakai M., Kono T.
Developmental and Comparative Immunology 154 105143 2024.5
Language：English Publishing type：Research paper (scientific journal) Publisher：Developmental and Comparative Immunology
Several immune-related genes, including Toll-like receptors (TLR), are associated with circadian rhythms in mammals. However, information on the circadian rhythmic expression of TLRs in fish is limited. In this study, we aimed to analyze the regulation of diel oscillations in the expression of TLR genes in Japanese medaka (Oryzias latipes). The expression analysis revealed diel expression patterns of tlr1, tlr5m, tlr21, and clock genes (bmal1 and clock1) under a 12 h light:12 h dark cycle. The clock gene response element (E-box) was identified in the transcriptional regulatory regions of tlr1, tlr5m, and tlr21. Moreover, overexpressed bmal1 and clock1 enhanced expression levels of tlr1, tlr5m, and tlr21 in medaka embryo (OLHdrR-e3) cells. The expression of tlr1, tlr5m, and tlr21 was significantly decreased in OLHdrR-e3 after generating a bmal1 knockdown using a morpholino oligo. These results indicate the regulation of the diel rhythmic expression of several fish TLRs by clock genes.
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Nishihara Aki, Sumiyoshi Takechiyo, Arakawa Koushiro, Morimoto Natsuki, Yasumoto Shinya, Kondo Masakazu, Kono Tomoya, Sakai Masahiro, Hikima Jun-ichi
Fish Pathology 58 ( 4 ) 175 - 179 2023.12
Authorship：Last author, Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：The Japanese Society of Fish Pathology
<i>Edwardsiella</i> bacteria infect Japanese medaka <i>Oryzias latipes</i>; however, the difference in susceptibility between <i>E. piscicida</i> and <i>E. anguillarum</i> is unknown. In this study, we performed challenge tests with medaka using four <i>Edwardsiella</i> strains, which were difficult to be identified by specific primers. The four strains were reclassified as three <i>E. piscicida</i> strains and one <i>E. anguillarum</i> strain using phylogenetic analysis. The immersion challenge demonstrated that medaka were more susceptible to <i>E. piscicida</i> than to <i>E. anguillarum</i>. Increases in the bacterial copy number in the infected medaka kidneys were correlated with the worsening of the external symptoms. Medaka is considered a valuable model organism for understanding the etiology of edwardsiellosis.
DOI： 10.3147/JSFP.58.175
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Interleukin-22 induces immune-related gene expression in the gills of Japanese medaka Oryzias latipes Reviewed International coauthorship
Watanabe M., Okamura Y., Kono T., Sakai M., Hikima J.i.
Developmental and Comparative Immunology 148 104916 2023.11
Authorship：Last author, Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：Developmental and Comparative Immunology
The cytokine interleukin (IL)-22 has been identified in several fish species; however, its functional significance in the gills of these fish species remains unclear. In this study, we analyzed the expression of proinflammatory cytokines, antimicrobial peptides, and IL-22 binding protein in the gills of wild-type and IL-22-knockout (IL-22 KO) medaka under dextran sulfate sodium-induced inflammation. We also produced medaka recombinant IL-22 (rIL-22) and analyzed the expression of immune-related genes in rIL-22-stimulated primary cell cultures from gills. The il1b, il6, tnfa, and hamp genes were significantly upregulated in wild-type gills upon dextran sulfate sodium stimulation compared with the naïve state but not in IL-22 KO gills. il22bp transcripts were barely detectable in the IL-22 KO medaka gills. However, the expression of il1b, il6, hamp, and il22bp was upregulated in rIL-22-stimulated gill cell culture. These results suggest IL-22 could be involved in immune responses through inflammatory cytokine and antimicrobial peptide production in fish gills.
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Transcriptomic Analysis of Cultured Kuruma Shrimp Primary Lymphoid Organ Cells for Identifying Key Cascades Hindering Sustainable Culture Reviewed International coauthorship
Tsuchiya A, Okamura Y, Kono T, Hikima J, Sakai M.
Fisheries Science 89 449 - 462 2023.5
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)
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Evolutional perspective and functional characteristics of interleukin-17 in teleosts
Okamura Y., Kono T., Sakai M., Hikima J.i.
Fish and Shellfish Immunology 132 ( 108496 ) 108496 - 108496 2023.1
Authorship：Last author, Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：Fish and Shellfish Immunology
Interleukin (IL)-17 is a proinflammatory cytokine and plays essential roles in adaptive and innate immune responses against bacterial and fungal infections. Especially in mammalian mucosal tissues, it is well known that innate immune responses via IL-17A and IL-17F, such as the production of antimicrobial peptides, are very important for microbiota control. In contrast, interesting insights into the functions of IL-17 have recently been reported in several teleost species, although little research has been conducted on teleost IL-17. In the present review, we focused on current insights on teleost IL-17 and speculated on the different or consensus parts of teleost IL-17 signaling compared to that of mammals. This review focuses on the role of teleost IL-17 in intestinal immunity. We expect that this review will encourage a further understanding of the roles and importance of IL-17 signaling in teleosts.
Books 【 display / non-display 】
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Inflammasome Biology
Jun-ichi Hikima, Natsuki Morimoto（ Role： Contributor , Chapter 15. Evolutive aspects of inflammasomes）
Elsevier 2022.12
Language：English Book type：Scholarly book
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Advances in Comparative Immunology
Teruyuki Nakanishi, Jun-ichi Hikima, Takashi Yada（ Role： Joint author , Innate Immunity in teleosts）
Springer 2018.6
Total pages：1107 Language：English Book type：Scholarly book
Teleosts and elasmobranchs are the lowest vertebrates that possess adaptive immunity akin to mammalian ones. However, the fish immune system is different from that of mammals, that is, a lack of bone marrow, lymph nodes and germinal center. Their immune system is also characterized by the fact that their surface is covered by live cells with mucus in direct contact with environmental water and the fact that the immune response is greatly affected by temperature. In this review we will describe the current understanding of the teleost immune system from a comparative viewpoint. We will also discuss the regulation of immune response by internal and external factors, since fish aquaculture is growing enormously worldwide, and understanding of the immune system is important for the development of immunological control of fish diseases.
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Prevention And Treatment Of Diseases, Section 2.2, Diagnosis – PCR Detection. In: Fish Diseases (Ed. Takashi Aoki), Food and Agricultural Sciences, Engineering and Technology Resources
Aoki T, Hikima J（ Role： Joint author）
Eolss Publishers/UNESCO, Co. Ltd., UK 2016.2
Language：English Book type：Textbook, survey, introduction
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Diseases Caused By Bacterial Pathogens In Saltwater, Section 5. Vibriosis. In: Fish Diseases (Ed. Takashi Aoki), Food and Agricultural Sciences, Engineering and Technology Resources
Takahashi Y, Hikima J（ Role： Joint author）
Eolss Publishers/UNESCO, Co. Ltd., UK 2016.2
Language：English Book type：Textbook, survey, introduction
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Fish And Shellfish Bio-Defense, Section 1. Innate Immunity In Fish. In: Fish Diseases (Ed. Takashi Aoki), Food and Agricultural Sciences, Engineering and Technology Resources
Aoki T, Hikima J（ Role： Joint author）
Eolss Publishers/UNESCO, Co. Ltd., UK 2016.2
Language：English Book type：Textbook, survey, introduction
Presentations 【 display / non-display 】
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エドワジエラ症原因菌の魚類感染へのパイロプトーシスの関与
引間順一
「集え、多分野研究者！」感染症キャンプin宮崎 2024.1.27
Event date： 2024.1.26 - 2024.1.28
Language：Japanese Presentation type：Oral presentation (general)
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Edwardsiella piscicida由来T&SS関連病原因子EvpPによる組織内増殖能及び宿主免疫応答の阻害
住吉竹千代・山本樹奈・出口 華・西原 輝・河野智哉・酒井正博・引間順一
「集え、多分野研究者！」感染症キャンプin宮崎 2024.1.27
Event date： 2024.1.26 - 2024.1.28
Language：Japanese Presentation type：Poster presentation
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Edwardsiella piscicida由来Ivyの宿主魚類に対する病原性
西原 輝・住吉竹千代・河野智哉・酒井正博・引間順一
「集え、多分野研究者！」感染症キャンプin宮崎 2024.1.27
Event date： 2024.1.26 - 2024.1.28
Language：Japanese Presentation type：Poster presentation
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メダカ鰭細胞におけるIL-17A/F−IL-17RC経路を介した免疫関連遺伝子の発現誘導
川口航世・河野智哉・酒井正博・引間順一
令和5年日本水産学会九州支部大会 2023.12.10
Event date： 2023.12.9 - 2023.12.10
Language：Japanese Presentation type：Oral presentation (general)
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mpeg1蛍光標識ノックイン・メダカにおける魚病細菌感染後の標識細胞の特徴
山本樹奈・出口 華・中神賢太郎・齊藤 暁・宮西 弘・河野智哉・酒井正博・引間順一・木下政人
令和5年日本水産学会九州支部大会 2023.12.10
Event date： 2023.12.9 - 2023.12.10
Language：Japanese Presentation type：Oral presentation (general)
Awards 【 display / non-display 】
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令和5年度日本水産学会九州支部会・学生優秀発表賞
2023.12 日本水産学会九州支部 Edwardsiella piscicida由来Ivyの病原性因子としての特性
西原 輝（指導者：引間順一）
Award type：Award from Japanese society, conference, symposium, etc.
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令和5年度日本水産学会九州支部会・学生優秀発表賞
2023.12 日本水産学会九州支部 mpeg1蛍光標識ノックイン・メダカにおける魚病細菌感染後の標識細胞の特徴
山本樹奈（指導者：引間順一）
Award type：Award from Japanese society, conference, symposium, etc.
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日本水産学会進歩賞
2020.3 日本水産学会
引間 順一
Award type：International academic award (Japan or overseas) Country：Japan
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Outstanding Contribution in Reviewing, Fish and Shellfish Immunology
2018.10 ELSEVIER
引間 順一
Award type：Honored in official journal of a scientific society, scientific journal Country：Netherlands
Fish and Shellfish Immunology, Outstanding Contribution in Reviewing, awarded October 2018 to Junichi Hikima in recognition of the contributions made to the quality of the ELSEVIER journal.
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日本魚病学会奨励賞
2018.3 日本魚病学会 魚類における自然免疫機構関連遺伝子に関する研究
引間 順一
Award type：Award from Japanese society, conference, symposium, etc. Country：Japan
Grant-in-Aid for Scientific Research 【 display / non-display 】
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魚類エドワジエラ症原因菌による宿主炎症性細胞死からの回避メカニズムの解明
Grant number：22H02423 2022.04 - 2026.03
日本学術振興会 科学研究費補助金 基盤研究(B)
引間 順一、酒井 正博
Authorship：Principal investigator Grant type：Competitive
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海水経験技術による回遊性水産有用魚種の成長・成熟促進機構の解明と応用
Grant number：23H02296 2023.04 - 2027.03
独立行政法人日本学術振興会 科学研究費補助金 基盤研究(B)
Authorship：Coinvestigator(s)
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宿主免疫系減弱化に関与するエドワジエラ症原因菌の病原性因子に関する研究
Grant number：23KJ1776 2023.04 - 2025.03
独立行政法人日本学術振興会 科学研究基金 特別研究員奨励費
Authorship：Principal investigator
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エビ類の株化細胞樹立に挑戦する
Grant number：20K20299 2017.04 - 2022.03
日本学術振興会 科学研究費補助金 挑戦的研究(開拓)
酒井 正博、引間順一、稲田 真理
Authorship：Coinvestigator(s)
細胞の機能解析やウイルスの研究を行うにあたり株化細胞の存在は必須である。1980年代中頃からエビ類においても長期培養可能な細胞株の樹立が試みられてきたが、未だ実現していない。本研究は、これまで試みが行われなかったエビ類の細胞の長期培養に最適なサプリメントの作製から、ヒトの株化細胞（ES細胞やiPS細胞）作製過程で使用されている最新技術（フィーダー細胞や遺伝子導入）を取り入れてクルマエビの細胞を株化させるまでを体系立てて行い、長期間培養可能または無限増殖能を持つエビ類の株化細胞を樹立することを目的としている。本研究は、応募者らが得意とする海産動物の細胞培養技術および最先端の遺伝子操作技術を駆使するエビ類初の挑戦的な内容となっており、エビ類の株化細胞樹立に成功すれば、エビ類の免疫・神経・内分泌系に関する基礎／応用研究が飛躍的に進むと思われる。さらに、産業レベルでは、現在、有効な疾病防除法がないエビ養殖におけるウイルス疾病蔓延・大量死といった問題解決への道が開ける可能性を秘めている。また、株化細胞を用いてエビの生体分子の機能解析が可能となり、無脊椎動物の細胞生物学に関する種々の知見を得る足掛かりとなる。
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魚類病原細菌由来の多糖類認識メカニズムを利用した魚病細菌感染防除技術の開発
Grant number：17H03863 2017.04 - 2021.03
日本学術振興会 科学研究費補助金 基盤研究(B)
引間 順一、木下 政人
Authorship：Principal investigator
養殖場重要魚種において魚病細菌による疾病発生状況は毎年甚大であり、その対策が急務である。これまで、魚病細菌感染症に対してリポ多糖(LPS)の免疫賦活効果が高いにもかかわらず、魚類における分子メカニズムは殆ど理解されてこなかった。そこで申請者は、LPSを認識する機構を明らかにすることで、細菌感染を防御する重要な遺伝子を特定することができると考えた。また、ノックアウト(KO)メダカを使用することでLPS認識メカニズムをより分かりやすく解明し、有用遺伝子を特定する。さらに、当該遺伝子を遺伝子アジュバントとして利用することで、これまでにないワクチン効果を発揮させる新規アジュバントの構築を試みる。
Other research activities 【 display / non-display 】
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国際学術雑誌 「Aquaculture」の Associate Editor
2022.04
国際学術誌「Aquaculture」投稿論文(83報)の編集、評価コメントの作成、および審査判定
