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Faculty of Agriculture Region of Marine Life Science |
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Professor |
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Related SDGs |
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HIKIMA Junichi
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Research Areas 【 display / non-display 】
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Life Science / Aquatic bioproduction science
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Life Science / Aquatic life science
Papers 【 display / non-display 】
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Nishihara A., Sumiyoshi T., Washim M.R., Yasumoto S., Kondo M., Kono T., Hikima J.i.
Aquaculture 608 742707 2025.9
Authorship：Last author, Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：Aquaculture
Edwardsiella piscicida (Enterobacteriaceae) is an important fish pathogen that causes edwardsiellosis in many species, including Japanese flounder (Paralichthys olivaceus), leading to economic losses in aquaculture. We investigated the direct inhibitory activity of IvyEp (Inhibitors of vertebrate lysozyme derived from E. piscicida) against host C-type lysozyme, and its role in host immune evasion. The results of functional assays confirmed that IvyEp significantly inhibited the activity of hen egg white lysozyme (HEWL), and kinetic analysis demonstrated that IvyEp acts as a competitive inhibitor. The ivy-deleted strain of E. piscicida exhibited reduced virulence in Japanese medaka (Oryzias latipes) and flounder infection models, highlighting its role in immune evasion and pathogenicity. IvyEp significantly suppressed lysozyme-like activity in the serum of Japanese flounder, yellowtail (Seriola quinqueradiata), and Nile tilapia (Oreochromis niloticus), suggesting broad-spectrum inhibition of C-type lysozymes across these species. Further assays confirmed that IvyEp significantly inhibited the lytic activity of purified flounder C-type lysozyme (rLyzC), supporting the hypothesis that IvyEp directly interferes with host lysozyme activity. Structural analysis predicted that IvyEp blocks the active site of rLyzC through electrostatic interactions, preventing substrate binding, and thus neutralizing rLyzC function. Co-immunoprecipitation revealed that IvyEp directly binds to HEWL and rLyzC. These findings provide new insights into the potential role of IvyEp in protecting E. piscicida from host immune responses and suggest its probable function as a universal C-type lysozyme inhibitor across different fish species.
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First report of Peroxiredoxin-5 (PRDX5) in Starry Flounder (Platichthys stellatus): Molecular features and expression analysis. Reviewed International coauthorship
Sohn MY, Choi KM, Kang G, Woo WS, Kim KH, Son HJ, Hikima J, Park CI.
Developmental & Comparative Immunology 19 105291 2024.11
Language：English Publishing type：Research paper (scientific journal)
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Accumulation and Phagocytosis of Fluorescently Visualized Macrophages Against Edwardsiella piscicida Infection in Established mpeg1.1‑Transgenic Japanese Medaka Oryzias latipes. Reviewed
Yamamoto J, Deguchi H, Sumiyoshi T, Nakagami K, Saito A, Miyanishi H, Kondo M, Kono T, Sakai M, Kinoshita M, Hikima J
Marine Biotechnology 26 ( 4 ) 658 - 671 2024.6
Authorship：Last author, Corresponding author Language：English Publishing type：Research paper (scientific journal)
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令和5年度日本水産学会九州支部の活動報告 Invited
酒井 正博, 引間 順一
日本水産学会誌 90 ( 3 ) 281 - 282 2024.5
Authorship：Last author, Corresponding author Language：Japanese Publishing type：Research paper (scientific journal) Publisher：公益社団法人 日本水産学会
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Comprehensive analysis of diel rhythmic expression of the medaka toll-like receptor gene family Reviewed
Hata T., Shimawaki H., Setoguchi S., Morimoto N., Hikima J., Sakai M., Kono T.
Developmental and Comparative Immunology 154 105143 2024.5
Language：English Publishing type：Research paper (scientific journal) Publisher：Developmental and Comparative Immunology
Several immune-related genes, including Toll-like receptors (TLR), are associated with circadian rhythms in mammals. However, information on the circadian rhythmic expression of TLRs in fish is limited. In this study, we aimed to analyze the regulation of diel oscillations in the expression of TLR genes in Japanese medaka (Oryzias latipes). The expression analysis revealed diel expression patterns of tlr1, tlr5m, tlr21, and clock genes (bmal1 and clock1) under a 12 h light:12 h dark cycle. The clock gene response element (E-box) was identified in the transcriptional regulatory regions of tlr1, tlr5m, and tlr21. Moreover, overexpressed bmal1 and clock1 enhanced expression levels of tlr1, tlr5m, and tlr21 in medaka embryo (OLHdrR-e3) cells. The expression of tlr1, tlr5m, and tlr21 was significantly decreased in OLHdrR-e3 after generating a bmal1 knockdown using a morpholino oligo. These results indicate the regulation of the diel rhythmic expression of several fish TLRs by clock genes.
Books 【 display / non-display 】
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Inflammasome Biology
Jun-ichi Hikima, Natsuki Morimoto（ Role： Contributor , Chapter 15. Evolutive aspects of inflammasomes）
Elsevier 2022.12
Language：English Book type：Scholarly book
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Advances in Comparative Immunology
Teruyuki Nakanishi, Jun-ichi Hikima, Takashi Yada（ Role： Joint author , Innate Immunity in teleosts）
Springer 2018.6
Total pages：1107 Language：English Book type：Scholarly book
Teleosts and elasmobranchs are the lowest vertebrates that possess adaptive immunity akin to mammalian ones. However, the fish immune system is different from that of mammals, that is, a lack of bone marrow, lymph nodes and germinal center. Their immune system is also characterized by the fact that their surface is covered by live cells with mucus in direct contact with environmental water and the fact that the immune response is greatly affected by temperature. In this review we will describe the current understanding of the teleost immune system from a comparative viewpoint. We will also discuss the regulation of immune response by internal and external factors, since fish aquaculture is growing enormously worldwide, and understanding of the immune system is important for the development of immunological control of fish diseases.
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Evolutive aspects of inflammasomes
Hikima J., Morimoto N.（ Role： Sole author）
Inflammasome Biology Fundamentals Role in Disease States and Therapeutic Opportunities 2022.1 （ ISBN:9780323972062, 9780323918022 ）
Language：English Book type：Scholarly book
Inflammasome is a cytosolic multiprotein complex, consisting of a NOD-like receptor as a sensor molecule, apoptosis-associated speck-like protein containing a caspase recruitment domain as an adaptor protein, and pro-caspase-1. Inflammasome activation induces caspase-1, which causes the processing of proinflammatory cytokines and induction of inflammatory cell death called pyroptosis in mammals. Pyroptosis also plays an important role in protecting the host from infection via cell death and extracellular release of cytokines. Recently, several inflammasome-related genes in teleosts have been identified, and the evolutionary conservation and/or differentiation of their functions between mammals and teleosts have also been elucidated. In this section, we summarize the current knowledge on the molecular structure and functional mechanism of inflammasome-related molecules in teleosts compared to mammals, and also discusses their evolutionary differences and extent of conservation.
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Prevention And Treatment Of Diseases, Section 2.2, Diagnosis – PCR Detection. In: Fish Diseases (Ed. Takashi Aoki), Food and Agricultural Sciences, Engineering and Technology Resources
Aoki T, Hikima J（ Role： Joint author）
Eolss Publishers/UNESCO, Co. Ltd., UK 2016.2
Language：English Book type：Textbook, survey, introduction
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Fish And Shellfish Bio-Defense, Section 1. Innate Immunity In Fish. In: Fish Diseases (Ed. Takashi Aoki), Food and Agricultural Sciences, Engineering and Technology Resources
Aoki T, Hikima J（ Role： Joint author）
Eolss Publishers/UNESCO, Co. Ltd., UK 2016.2
Language：English Book type：Textbook, survey, introduction
Presentations 【 display / non-display 】
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Edwardsiella piscicida由来病原因子EvpPによる魚類パイロトーシスの阻害
住吉竹千代、西原 輝、河野智哉、酒井正博、引間順一
令和7年日本水産学会春季大会 2025.3.27
Event date： 2025.3.26 - 2025.3.29
Language：Japanese Presentation type：Oral presentation (general)
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ヒラメC型リゾチーム溶菌活性を阻害するEdwardsiella piscicida由来Ivy
西原 輝、住吉竹千代、安本信哉、近藤昌和、河野智哉、引間順一
令和7年日本水産学会春季大会 2025.3.27
Event date： 2025.3.26 - 2025.3.29
Language：Japanese Presentation type：Oral presentation (general)
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時計遺伝子Bmal1変異メダカにおける免疫応答
羽田宗彦、平吉美優、津田和忠、宮西 弘、引間順一、河野智哉
令和6年日本水産学会秋季大会 2024.9.26
Event date： 2024.9.24 - 2024.9.26
Language：Japanese Presentation type：Oral presentation (general)
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ヒラメC型リゾチーム溶菌活性に対するEdwardsiella piscicida由来Ivyの阻害能
西原 輝、住吉竹千代、安本信哉、近藤昌和、河野智哉、引間順一
令和6年日本水産学会秋季大会 2024.9.26
Event date： 2024.9.24 - 2024.9.26
Language：Japanese Presentation type：Oral presentation (general)
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メダカ組換えTNFαの作製および制御性細胞死の誘導
中岡侑莉乃、石原怜於、西原 輝、河野智哉、引間順一
令和6年日本水産学会秋季大会 2024.9.26
Event date： 2024.9.24 - 2024.9.26
Language：Japanese Presentation type：Oral presentation (general)
Awards 【 display / non-display 】
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令和5年度日本水産学会九州支部会・学生優秀発表賞
2023.12 日本水産学会九州支部 Edwardsiella piscicida由来Ivyの病原性因子としての特性
西原 輝（指導者：引間順一）
Award type：Award from Japanese society, conference, symposium, etc.
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令和5年度日本水産学会九州支部会・学生優秀発表賞
2023.12 日本水産学会九州支部 mpeg1蛍光標識ノックイン・メダカにおける魚病細菌感染後の標識細胞の特徴
山本樹奈（指導者：引間順一）
Award type：Award from Japanese society, conference, symposium, etc.
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日本水産学会進歩賞
2020.3 日本水産学会
引間 順一
Award type：International academic award (Japan or overseas) Country：Japan
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Outstanding Contribution in Reviewing, Fish and Shellfish Immunology
2018.10 ELSEVIER
引間 順一
Award type：Honored in official journal of a scientific society, scientific journal Country：Netherlands
Fish and Shellfish Immunology, Outstanding Contribution in Reviewing, awarded October 2018 to Junichi Hikima in recognition of the contributions made to the quality of the ELSEVIER journal.
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日本魚病学会奨励賞
2018.3 日本魚病学会 魚類における自然免疫機構関連遺伝子に関する研究
引間 順一
Award type：Award from Japanese society, conference, symposium, etc. Country：Japan
Grant-in-Aid for Scientific Research 【 display / non-display 】
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魚類エドワジエラ症原因菌による宿主炎症性細胞死からの回避メカニズムの解明
Grant number：23K23688 2024.04 - 2026.03
独立行政法人日本学術振興会 科学研究費基金 基盤研究(B)
Authorship：Principal investigator
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海水経験技術による回遊性水産有用魚種の成長・成熟促進機構の解明と応用
Grant number：23H02296 2023.04 - 2027.03
独立行政法人日本学術振興会 科学研究費補助金 基盤研究(B)
Authorship：Coinvestigator(s)
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宿主免疫系減弱化に関与するエドワジエラ症原因菌の病原性因子に関する研究
Grant number：23KJ1776 2023.04 - 2025.03
独立行政法人日本学術振興会 科学研究基金 特別研究員奨励費
Authorship：Principal investigator Grant type：Competitive
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魚類エドワジエラ症原因菌による宿主炎症性細胞死からの回避メカニズムの解明
Grant number：22H02423 2022.04 - 2024.03
日本学術振興会 科学研究費補助金 基盤研究(B)
引間 順一、酒井 正博
Authorship：Principal investigator Grant type：Competitive
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エビ類の株化細胞樹立に挑戦する
Grant number：20K20299 2017.04 - 2022.03
日本学術振興会 科学研究費補助金 挑戦的研究(開拓)
酒井 正博、引間順一、稲田 真理
Authorship：Coinvestigator(s)
細胞の機能解析やウイルスの研究を行うにあたり株化細胞の存在は必須である。1980年代中頃からエビ類においても長期培養可能な細胞株の樹立が試みられてきたが、未だ実現していない。本研究は、これまで試みが行われなかったエビ類の細胞の長期培養に最適なサプリメントの作製から、ヒトの株化細胞（ES細胞やiPS細胞）作製過程で使用されている最新技術（フィーダー細胞や遺伝子導入）を取り入れてクルマエビの細胞を株化させるまでを体系立てて行い、長期間培養可能または無限増殖能を持つエビ類の株化細胞を樹立することを目的としている。本研究は、応募者らが得意とする海産動物の細胞培養技術および最先端の遺伝子操作技術を駆使するエビ類初の挑戦的な内容となっており、エビ類の株化細胞樹立に成功すれば、エビ類の免疫・神経・内分泌系に関する基礎／応用研究が飛躍的に進むと思われる。さらに、産業レベルでは、現在、有効な疾病防除法がないエビ養殖におけるウイルス疾病蔓延・大量死といった問題解決への道が開ける可能性を秘めている。また、株化細胞を用いてエビの生体分子の機能解析が可能となり、無脊椎動物の細胞生物学に関する種々の知見を得る足掛かりとなる。
Other research activities 【 display / non-display 】
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2024 Mid-career Researcher Program (Type 1): Development of vaccine adjuvants for Starry Flounder (Platichthys stellatus)-derived protein as an active ingredient
2024.07 - 2029.04
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国際学術雑誌 「Aquaculture」の Associate Editor
2022.04
国際学術誌「Aquaculture」投稿論文(83報)の編集、評価コメントの作成、および審査判定
