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Affiliation |
Faculty of Agriculture Region of Animal and Plant Biosciences |
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Title |
Associate Professor |
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Related SDGs |
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HIRANO Tomonari
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Degree 【 display / non-display 】
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Doctor (Agriculture) ( 2006.3 Chiba University )
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修士（農学） ( 2003.3 千葉大学 )
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学士（農学） ( 2001.3 千葉大学 )
Research Interests 【 display / non-display 】
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Male gamete
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Mutation
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DNA double strand break
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Flower development
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Double fertilization
Research Areas 【 display / non-display 】
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Environmental Science/Agriculture Science / Horticultural science
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Environmental Science/Agriculture Science / Science in plant genetics and breeding
Papers 【 display / non-display 】
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Tomonari Hirano, Muneaki Murata, Yurie Watarikawa, Yoichiro Hoshino, Tomoko Abe, Hisato Kunitake
Plant Reproduction 37 ( 3 ) 355 - 363 2024.9
Authorship：Lead author, Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：Plant Reproduction
Key message: In Cyrtanthus mackenii, development of embryo and endosperm were differentially affected by fertilization of male gametes with DNA damage and mutations. Abstract: Pollen irradiation with ionizing radiations has been applied in plant breeding and genetic research, and haploid plant induction has mainly been performed by male inactivation with high-dose irradiation. However, the fertilization process of irradiated male gametes and the early development of embryo and endosperm have not received much attention. Heavy-ion beams, a type of radiation, have been widely applied as effective mutagens for plants and show a high mutation rate even at low-dose irradiation. In this study, we analyzed the effects of male gametes of Cyrtanthus mackenii irradiated with a carbon-ion beam at low doses on fertilization. In immature seeds derived from the pollination of irradiated pollen grains, two types of embryo sacs were observed: embryo sac with a normally developed embryo and endosperm and embryo sac with an egg cell or an undivided zygote and an endosperm. Abnormalities in chromosome segregation, such as chromosomal bridges, were observed only in the endosperm nuclei, irrespective of the presence or absence of embryogenesis. Therefore, in Cyrtanthus, embryogenesis is strongly affected by DNA damage or mutations in male gametes. Moreover, various DNA contents were detected in the embryo and endosperm nuclei, and endoreduplication may have occurred in the endosperm nuclei. As carbon-ion irradiation causes chromosomal rearrangements even at low doses, pollen irradiation can be an interesting tool for studying double fertilization and mutation heritability.
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Koga Y., Setoguchi Y., Sugamoto K., Goto Y., Hirano T., Kunitake H.
Plants 13 1864 2024.7
Language：English Publishing type：Research paper (scientific journal) Publisher：Plants
The leaves and branches of rabbiteye blueberry are rich in proanthocyanidins, which are thought to have different physiological activities depending on their structure and degree of polymerization. In this study, we analyzed the constituents of the leaves and branches of rabbiteye blueberry to determine the seasonal variations in polyphenol and proanthocyanidin (PAC) contents as well as their mean degrees of polymerization (mDP). Total PAC content was determined using two methods: The p-dimethylaminocinnamaldehyde (DMACA) method, which measures monomeric PAC, showed an increase from spring to summer in both leaves and branches. On the other hand, using the butanol/HCl method, which measures only polymerized PAC, the PAC content of leaves increased from spring to summer but those of branches remained low throughout the year, showing no significant increase or decrease. Furthermore, analysis of the mDP of PAC showed increases from spring to summer in the leaves of ‘Kunisato 35 gou’. Although the highest value (8.0) was observed in October, values around 4 remained throughout the year in the branches. Since differences in polymerization degree affect absorption in the body and physiological properties such as antioxidant capacity, selecting the appropriate harvest time and plant organs for each purpose is expected to ensure the quality of processed blueberry foods.
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Effects of fertilization of male gametes with heavy-ion beam irradiation on embryo and endosperm development in Cyrtanthus mackenii Reviewed International journal
Makiko Shii, Yuki Kajiya, Muneaki Murata, Tomoko Abe, Hisato Kunitake, Tomonari Hirano
CYTOLOGIA 89 ( 2 ) 133 - 139 2024.6
Authorship：Last author, Corresponding author Language：English Publishing type：Research paper (scientific journal)
DOI： https://doi.org/10.1508/cytologia.89.133
Other Link： https://www.jstage.jst.go.jp/article/cytologia/89/2/89_D-24-00011/_article/-char/en
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Changes in Carotenoids and Polyphenols during the Growth Stages of Orange-Fleshed Sweet Potato (Ipomoea batatas (L.) Lam.) Reviewed
Yuno Setoguchi, Yosuke Narasako, Tomonari Hirano, Motoyasu Otani, Hisato Kunitake
Horticulturae 10 ( 6 ) 629 2024.6
Publishing type：Research paper (scientific journal)
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Principles of amyloplast replication in the ovule integuments of Arabidopsis thaliana. Reviewed
Fujiwara MT, Yoshioka Y, Kazama Y, Hirano T, Niwa Y, Moriyama T, Sato N, Abe T, Yoshida S, Itoh RD
Plant physiology 196 ( 1 ) 137 - 152 2024.6
Language：English Publishing type：Research paper (scientific journal)
Books 【 display / non-display 】
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國武 久登 , 執行 正義, 平野 智也, 神戸 敏成, 貴島 祐治 , 三吉 一光, 大谷 基泰, 八幡 昌紀, 星野 洋一郎, 中野 優 , 佐々 英徳, 三柴 啓一郎, 山本 伸一 (農学)（ Role： Joint editor）
講談社 2023.5 （ ISBN:9784065308158 ）
Language：Japanese Book type：Textbook, survey, introduction
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最新農業技術 花卉〈vol.5〉切り花で新境地をひらくアジサイ、ダリア、ラナンキュラス、ユーストマ
阿部 知子， 平野 智也（ Role： Joint author , 突然変異（イオンビーム）育種）
農山漁村文化協会 2013.3
Language：Japanese Book type：Scholarly book
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Pollen: Structure, Types and Effects
Tomonari Hirano, Yoichiro Hoshino（ Role： Joint author , Capture of male gamete dynamics in pollen tubes）
Nova Science Publishers 2010.7
Language：English Book type：Scholarly book
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日本の植物園における生物多様性保全
平野 智也， 神戸 敏成（ Role： Joint author , 日本産希少植物の繁殖および長期保存）
日本植物園協会・国立博物館筑波実験植物園・植物園自然保護国際機構 2007.3
Language：Japanese Book type：Scholarly book
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Floriculture, Ornamental and Plant Biotechnology: advances and topical issues
Tomonari Hirano, Keiko Ishikawa, Masahiro Mii（ Role： Joint author , Advances in Orchid Cryopreservation）
Global Science Book 2006.6
Language：English Book type：Scholarly book
MISC 【 display / non-display 】
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Development of breeding technology by using heavy-ion beam Invited Reviewed
Ion-Beam Breeding Research Group, Abe Tomoko, Kazama Yusuke, Hirano Tomonari
Breeding Research 25 ( 2 ) 170 - 176 2023.12
Authorship：Last author Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (scientific journal) Publisher：Japanese Society of Breeding
DOI： 10.1270/jsbbr.23j13
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イオンビームを用いた花き植物の品種改良 Invited Reviewed
平野智也
アグリバイオ 6 ( 12 ) 14 - 18 2022.10
Authorship：Lead author, Corresponding author Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media)
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Mutagenic Effects of Heavy-Ion Beam Irradiation to Plant Genome Reviewed
Tomonari Hirano, Yusuke Kazama, Hisato Kunitake, Tomoko Abe
CYTOLOGIA 87 ( 1 ) 3 - 6 2022.3
Authorship：Lead author, Corresponding author Language：English Publishing type：Article, review, commentary, editorial, etc. (scientific journal)
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Chromosomal rearrangement: From induction by heavy-ion irradiation to in vivo engineering by genome editing Reviewed
Yusuke Kazama, Tomonari Hirano, Tomoko Abe, Sachihiro Matsunaga
Cytologia 83 ( 2 ) 125 - 128 2018.6
Language：English Publishing type：Article, review, commentary, editorial, etc. (scientific journal)
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重イオンビームで広がる花きの新品種作出 Invited Reviewed
平野 智也，市田 裕之，阿部 知子
化学と生物 55 ( 11 ) 775 - 782 2017.10
Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (scientific journal)
Presentations 【 display / non-display 】
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新たな花粉照射利用に向けた受精解析 Invited
平野智也, 阿部知子, 國武久登
理研シンポジウム「重イオンビームによる環境変化に対応する品種の育成」 2025.1.23
Event date： 2025.1.23 - 2025.1.24
Language：Japanese Presentation type：Oral presentation (invited, special)
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ランの遺伝資源保全に向けた種子保存 Invited
平野 智也
第３回 種子・胞子・組織培養を使った保全フォーラム 2024.12.20
Event date： 2024.12.20
Language：Japanese Presentation type：Oral presentation (general)
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花器官サイズ調節遺伝子による花弁形態制御
植村亮介、Vuong Quoc Nhat、阿部知子、國武久登、平野智也
日本育種学会 第19回九州育種談話会 2024.12.9
Event date： 2024.12.9
Language：Japanese Presentation type：Poster presentation
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サツマイモ‘べにはるか’の十二倍体の育成とその特徴
瀬戸口優乃、大村諒、奈良迫祐介、大谷基泰、平野智也、國武久登
日本育種学会 第19回九州育種談話会 2024.12.9
Event date： 2024.12.9
Language：Japanese Presentation type：Poster presentation
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サツマイモ茎部のポリフェノール含量は基腐病の発病度に影響する
瀬戸口優乃、福留春香、井上遼太郎、 廣中佑真、山村実央、竹下稔、大谷基泰、平野智也、國武久登
日本育種学会 第19回九州育種談話会 2024.12.9
Event date： 2024.12.9
Language：Japanese Presentation type：Poster presentation
Awards 【 display / non-display 】
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日本育種学会賞
2023.3 日本育種学会 重イオンビームによる育種技術の開発
重イオン育種技術開発グループ（阿部知子、風間裕介、平野智也）
Award type：Award from Japanese society, conference, symposium, etc.
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宮崎銀行ふるさと振興助成事業
2025.3 みやぎん経済研究所
平野智也
Award type：Award from publisher, newspaper, foundation, etc.
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RIKEN Accelerator Progress Report 2023 Vol. 57 HIGHLIGHTS OF THE YEAR
2024.12 理化学研究所仁科加速器研究センター加速器年次報告編集委員会 Characterization of a novel mutant with inhibition of storage root formation in sweet potato
H. Park, T. Abe, H. Kunitake, T. Hirano
Award type：Honored in official journal of a scientific society, scientific journal Country：Japan
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平成28年度園芸学会年間優秀論文賞
2017.3 園芸学会
澤田 倫平, 平野 智也, 飯牟禮 和彦, 阿部 知子, 尾崎 行生
Award type：Honored in official journal of a scientific society, scientific journal Country：Japan
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RIKEN Accelerator Progress Report 2015 Vol. 49 HIGHLIGHTS OF THE YEAR
2017.3 仁科加速器研究センター加速器年次報告編集委員会
A. Hisamura, D. Mine, T. Takeda, T. Abe, Y. Hayashi, T. Hirano
Award type：Honored in official journal of a scientific society, scientific journal Country：Japan
Grant-in-Aid for Scientific Research 【 display / non-display 】
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人為誘発非還元性雄性配偶子を活用した倍数性多様化法の開発
Grant number：20K06035 2020.04 - 2024.03
独立行政法人日本学術振興会 科学研究費補助金 基盤研究(C)
Authorship：Principal investigator
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染色体微細加工で逆位・転座が植物ゲノムに与える影響を見る
Grant number：20H03297 2020.04 - 2023.03
独立行政法人日本学術振興会 科学研究費補助金 基盤研究(B)
Authorship：Coinvestigator(s)
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Establishment of analysis method for double fertilization process using unreduced male gamete
Grant number：17K15223 2017.04 - 2020.03
Grant-in-Aid for Scientific Research Grant-in-Aid for Young Scientists(B)
Authorship：Principal investigator
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高等植物における異型精細胞形成機構の解明
Grant number：22780034 2010.04 - 2013.03
科学研究費補助金 若手研究(B)
Authorship：Principal investigator
キルタンサスでは、花粉管伸長過程で一組の精細胞が異型化し、雄原細胞分裂直後の表層微小管構成時に蓄積の差異が生じる事が明らかとなった。精細胞形成の分子基盤情報を得るために遺伝子解析を行い、異型化特異的ステージの前後で発現量が変化する遺伝子の候補リストが得られた。花粉に重イオンビーム照射することで、雄性配偶子の研究に有用な新たな材料を作出するとともに、雄性配偶子のDNA損傷応答機構を明らかにした。
Other research activities 【 display / non-display 】
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Chrysanthemum x morifolium Ramat. ‘長崎SYC1’
2021.03
品種登録 出願番号35321
育成者：久村麻子、諸岡淳司、阿部知子、林依子、平野智也、石井公太郎 -
Chrysanthemum x morifolium Ramat. ‘長崎SWC1’
2019.03
品種登録 登録番号28537
育成者：久村麻子、諸岡淳司、阿部知子、林依子、平野智也 -
Prunus L. '仁科知花'
2016.06
品種登録 出願番号31199
育成者：石井重久、阿部知子、林 依子、平野智也 -
Chrysanthemum x morifolium Ramat. ‘白涼’
2015.03
品種登録 登録番号26306
育成者：峯大樹、岳田司、久村麻子、阿部知子、平野智也、林依子 -
Prunus L. ‘仁科小町’
2012.09 - 2022.06
品種登録 出願番号27386
育成者：石井重久、阿部知子、林依子、福西暢尚、平野智也
Available Technology 【 display / non-display 】
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微細操作技術を活用した植物の受精機構に関する研究
突然変異育種法の効率化に関する研究
ラン科植物の長期遺伝資源保存法の開発Home Page： 植物遺伝育種学研究室
Related fields where technical consultation is available：・花き植物を中心とした突然変異育種
・植物遺伝資源の保存、植物組織培養による増殖
・突然変異遺伝子の同定、単離Message：・共同研究の希望テーマ：花き園芸植物の品種改良
・少数細胞解析技術の開発ニーズがあれば、ぜひご連絡ください。