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Affiliation |
Faculty of Agriculture Region of Animal and Plant Biosciences |
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Associate Professor |
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Related SDGs |
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HIRANO Tomonari
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Degree 【 display / non-display 】
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Doctor (Agriculture) ( 2006.3 Chiba University )
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修士（農学） ( 2003.3 千葉大学 )
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学士（農学） ( 2001.3 千葉大学 )
Research Interests 【 display / non-display 】
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Mutation
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DNA double strand break
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Flower development
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Double fertilization
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Male gamete
Research Areas 【 display / non-display 】
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Environmental Science/Agriculture Science / Horticultural science
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Environmental Science/Agriculture Science / Science in plant genetics and breeding
Papers 【 display / non-display 】
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Nagalla A.D., Ishii K., Hirano T., Ohbu S., Shirakawa Y., Kazama Y., Abe T.
Journal of Plant Research 139 ( 4 ) 629 - 644 2026.7
Language：English Publishing type：Research paper (scientific journal) Publisher：Journal of Plant Research
Heavy-ion beam irradiation is recognized as a powerful mutagenesis technique because it induces high mutation frequencies, causes minimal damage to other traits, and has the potential to produce large DNA-fragmentation mutants. Aneuploid cells may have an additional or fewer number of chromosomes compared to their wild-type. The mutant line of flower late pltioleless1 (flpl1) is an ion beam-generated, Arabidopsis thaliana Col-0 based, aneuploid mutant line confirmed by whole genome sequence analysis, DNA quantity measurement using flow cytometry analysis and microscopic observations of an additional chromosome. The mutant shows abnormal leaf shapes, petioleless rosette leaves, elevated trichome bases and later-flowering phenotypes. Segregation analysis confirmed that the amount of additional DNA correlates with the observed phenotype as mutants with intermediate phenotypes possess moderate amounts of additional DNA. A set of candidate causal genes was identified using up and downregulated differentially expressed genes in transcriptome analysis. Gene ontology analysis further supports the mutant’s phenotypic characteristics and provides insights into its functional priorities, including the promotion of cellular structure and energy production. Meanwhile, the downregulation of senescence-related gene ontology terms validates its extended vegetative phase. The whole genome sequence-based chromosome rearrangement prediction and sequence coverage map analysis established the expected chromosomal model for flpl1. Ion beam mutagenesis generates aneuploidy and segmental aneuploidy mutants that may possess fused DNA-fragmented chromosomes.
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Evaluation of viable regions for successful cryopreservation of orchid protocorms Reviewed
Tomonari Hirano, Ayaka Yamamoto, Hiroka Miyagi, Soichiro Tsuda, Tomohisa Yukawa, Hisato Kunitake
Plant Biotechnology 43 ( 2 ) 175 - 180 2026.6
Authorship：Lead author, Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：日本植物バイオテクノロジー学会
In Orchidaceae, the protocorm, which forms after seed germination, serves as a crucial genetic resource for plant preservation and breeding. To establish a long-term conservation method for the protocorm, in this study, we developed cryopreservation methods. Protocorms of <i>Bletilla striata</i>, a terrestrial orchid species, were precultured for 3 days in a medium containing 0.3 M sucrose and then cryopreserved using vitrification. Although viable cell staining indicated the presence of many surviving cells in the protocorms, the regrowth rate was extremely low. We categorized the stained regions based on the presence or absence of staining in the shoot apical meristem (SAM). The staining rate in the SAM region was low after cryopreservation, which was considered the cause of the low regrowth rate. To enable cryopreservation of the protocorms, the V-Cryo-plate method was adopted, and the treatment times for the solutions used in dehydration were investigated. Treatment times of 45 min and 2 h for the loading and vitrification solutions were found to be appropriate, respectively. Furthermore, an investigation of the effect of adding plant growth regulators to the preculture medium showed that adding 0.1 μM 1-naphthalene acetic acid resulted in a high SAM survival rate of 88% and regrowth rate of 87%, comparable to that observed with survival cell staining. Therefore, survival in the SAM region is important for regrowth in cryopreserved protocorms. Confirmation of survival regions is an important indicator for bridging the gap between the survival and regrowth rates.
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Miniaturization responses of shallot bulbs from true seed to planting density and paclobutrazol treatments Reviewed International coauthorship
SYAM'UN E., ULFA F., HARING F., SJAHRIL R., MANTJA K., DERMAWAN R., FARIED M., HIRANO T., TAMBUNG A., APRILIA R.N.
Notulae Botanicae Horti Agrobotanici Cluj Napoca 54 ( 1 ) 14914 - 14914 2026.4
Language：English Publishing type：Research paper (scientific journal) Publisher：Notulae Botanicae Horti Agrobotanici Cluj Napoca
The production of mini shallot bulbs from true seed offers potential advantages for efficient propagation and high-quality seed systems. This study evaluated the responses of shallot growth and bulb miniaturization to planting density and paclobutrazol root-dipping under greenhouse conditions. The experiment was arranged in a split-plot design with three planting densities (100, 200, and 300 plants m⁻B) as main plots and four paclobutrazol concentrations (0, 150, 300, and 450 mg L⁻C) as subplots. Growth traits, yield components, biochemical attributes, and geometric characteristics were analysed using ANOVA, regression, and correlation analyses. Paclobutrazol temporarily reduced plant height and leaf number during early growth but did not significantly affect bulb size, bulb weight, or pigment composition at harvest. In contrast, planting density markedly influenced plant morphology and yield distribution. Increasing density significantly decreased bulb diameter, fresh weight, and biomass per plant, while increasing the proportion of mini bulbs and single-bulb formations. Leaf pigment stability across treatments indicated no evidence of physiological stress associated with either factor. Regression analysis further showed that planting density exhibited stronger predictive relationships with yield parameters than paclobutrazol concentration. Overall, the findings indicate that bulb miniaturization in true-seed shallot is primarily associated with planting density rather than paclobutrazol application, highlighting high-density planting as a practical approach for mini-bulb production systems.
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Toshima Saki, Egashira Kodai, Mimura Makiko, Saeki Yuichi, Hirano Tomonari, Kunitake Hisato
Plant Molecular Biology Reporter 44 56 2026.2
Language：English Publishing type：Research paper (scientific journal) Publisher：Springer Science and Business Media LLC
Widely distributed from East Asia to Australia, Rubus parvifolius is the only known wild Rubus species that naturally grows throughout the Japanese Archipelago, including small southern islands. R. parvifolius has been attracting attention as a genetic resource in Asia for breeding raspberries with enhanced tolerance to environmental stresses. Amami Oshima and Kakeroma, two of Japan’s Amami Islands, exhibit high plant diversity due to significant species endemism, reflecting the islands' isolated biogeographical history. This provides a rationale for focusing on Amami Oshima and Kakeroma Islands as a suitable system for studying wild Rubus species. In this study, we conducted ploidy assessments and analyzed Simple Sequence Repeat (SSR) markers in 24 accessions of R. parvifolius and other wild Rubus species (R. grayanus and R. croceacanthus) from those islands. Ploidy evaluation using flow cytometry and chromosome observation revealed that 11 of the 24 accessions (45.8%) were triploid. In addition, 52 SSR markers were employed, of which 20 were selected for analysis. Principal coordinate analysis (PCoA) using these SSR markers showed that diploid and triploid individuals did not form distinct clusters. Although this study was preliminary and based on a limited sample size, a relatively frequent occurrence of polyploid individuals was observed among the R. parvifolius accessions examined from Amami Oshima and Kakeroma. Our results provide baseline information on the occurrence of polyploid individuals in wild species in island systems.
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Narasako Yosuke, Setoguchi Yuno, Fukutome Haruka, Hirano Tomonari, Otani Motoyasu, Takeshita Minoru, Kunitake Hisato
Horticulturae 11 ( 12 ) 1439 2025.11
Language：English Publishing type：Research paper (scientific journal) Publisher：MDPI AG
Foot rot, a disease caused by the fungal plant pathogen Diaporthe destruens, has been a major problem throughout East Asia. In major sweet-potato-producing regions, developing sweet potato cultivars that are resistant to foot rot has become an urgent priority. The possibility of selecting resistant cultivars by using polyphenols in the stems as markers was recently suggested, but this selection method has not been tested in the crossbreeding of sweet potato cultivars. In this study, we crossed the sweet potato cultivars ‘Konaishin’ and ‘Tamaakane’ (each of which is resistant to foot rot), analyzed the polyphenols in the stems of the resulting hybrid lines, and evaluated the possibility of selecting resistant lines. As a result, KT No. 7 and KT No. 8 showed similar or lower total polyphenol contents (145.9 and 112.9 mg GAE 100 g−1 FW, respectively) compared to ‘Tamaakane’ (142.2 mg GAE 100 g−1 FW). The selected line, KT No. 7, exhibited the highest resistance among the hybrids when it was directly inoculated with the foot rot pathogen using stems as test material, showing a disease severity value of 1.8, which was substantially lower than that of ‘Tamaakane’ (50.0). These results suggest that stem polyphenol content has potential as a marker for identifying promising candidates with foot rot resistance, although its predictive value may vary depending on genetic and environmental factors. This approach may help improve the efficiency of foot rot resistance screening in sweet potato breeding programs.ms.
Books 【 display / non-display 】
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國武 久登 , 執行 正義, 平野 智也, 神戸 敏成, 貴島 祐治 , 三吉 一光, 大谷 基泰, 八幡 昌紀, 星野 洋一郎, 中野 優 , 佐々 英徳, 三柴 啓一郎, 山本 伸一 (農学)（ Role： Joint editor）
講談社 2023.5 （ ISBN:9784065308158 ）
Language：Japanese Book type：Textbook, survey, introduction
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最新農業技術 花卉〈vol.5〉切り花で新境地をひらくアジサイ、ダリア、ラナンキュラス、ユーストマ
阿部 知子， 平野 智也（ Role： Joint author , 突然変異（イオンビーム）育種）
農山漁村文化協会 2013.3
Language：Japanese Book type：Scholarly book
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Capture of male gamete dynamics in pollen tubes
Hirano T., Hoshino Y.（ Role： Sole author）
Pollen Structure Types and Effects 2010.12 （ ISBN:9781616686697 ）
The process of double fertilization involves 2 sperm cells that are delivered into female gametes via pollen tubes. To analyze this process, we utilized the bicellular pollen species Alstroemeria and Cyrtanthus, which produce 2 sperm cells in the pollen tubes. Novel procedures have been developed for the in vitro observation of pollen tubes under precise control conditions, for flow cytometric analysis with single cell manipulation, and for immunocytochemical analysis to detect cytoskeletons. Using these unique methods, male gamete dynamics have been elucidated during pollen tube development. In particular, we have focused on male germ unit (MGU) defining that the generative cells or the pair of sperm cells in pollen tubes or pollen grains are closely associated with the vegetative nucleus. This MGU is proposed to function as a vehicle for the transmission of male gametes to the female gametes and to participate in the fusion with the target female cell during fertilization. In this chapter, recent insights into male gamete dynamics using novel procedures are discussed. © 2010 by Nova Science Publishers, Inc. All rights reserved.
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Pollen: Structure, Types and Effects
Tomonari Hirano, Yoichiro Hoshino（ Role： Joint author , Capture of male gamete dynamics in pollen tubes）
Nova Science Publishers 2010.7
Language：English Book type：Scholarly book
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日本の植物園における生物多様性保全
平野 智也， 神戸 敏成（ Role： Joint author , 日本産希少植物の繁殖および長期保存）
日本植物園協会・国立博物館筑波実験植物園・植物園自然保護国際機構 2007.3
Language：Japanese Book type：Scholarly book
MISC 【 display / non-display 】
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Plant Breeding Accelerated by Heavy-Ion Mutagenesis Reviewed
Tomoko Abe, Ryouhei Morita, Yoriko Hayashi, Tomonari Hirano
Plant Breeding and Genetics Newsletter 55 10 - 14 2025.7
Authorship：Last author Language：English Publishing type：Article, review, commentary, editorial, etc. (scientific journal)
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重イオンビーム育種技術の高度化 Invited
平野智也
調査月報 383 2 - 6 2025.4
Authorship：Lead author Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media)
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Development of breeding technology by using heavy-ion beam Invited Reviewed
Ion-Beam Breeding Research Group, Abe Tomoko, Kazama Yusuke, Hirano Tomonari
Breeding Research 25 ( 2 ) 170 - 176 2023.12
Authorship：Last author Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (scientific journal) Publisher：Japanese Society of Breeding
DOI： 10.1270/jsbbr.23j13
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イオンビームを用いた花き植物の品種改良 Invited Reviewed
平野智也
アグリバイオ 6 ( 12 ) 14 - 18 2022.10
Authorship：Lead author, Corresponding author Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media)
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Mutagenic Effects of Heavy-Ion Beam Irradiation to Plant Genome Reviewed
Tomonari Hirano, Yusuke Kazama, Hisato Kunitake, Tomoko Abe
CYTOLOGIA 87 ( 1 ) 3 - 6 2022.3
Authorship：Lead author, Corresponding author Language：English Publishing type：Article, review, commentary, editorial, etc. (scientific journal)
Presentations 【 display / non-display 】
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重イオンビーム花粉照射を利用したDNA量多様化法の開発
松井空，Numnueng Wattanakasemsakun，椎槙子，阿部知子，國武久登，平野智也
園芸学会令和8年度春季大会 2026.3.22
Event date： 2026.3.21 - 2026.3.22
Language：Japanese Presentation type：Poster presentation
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露地開花性サツマイモにおける倍数性キメラ植物の形態的および遺伝的特徴
福島郁菜，瀬戸口優乃，奈良迫洋介，大谷基泰，平野智也，國武久登
園芸学会令和8年度春季大会 2026.3.22
Event date： 2026.3.21 - 2026.3.22
Language：Japanese Presentation type：Poster presentation
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サツマイモの貯蔵根におけるアントシアニンやカロテノイドに及ぼす生育ステージの影響
廣中佑真，瀬戸口優乃，奈良迫洋介，大谷基泰，平野智也，國武久登
園芸学会令和8年度春季大会 2026.3.22
Event date： 2026.3.21 - 2026.3.22
Language：Japanese Presentation type：Poster presentation
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シロイヌナズナの珠皮アミロプラストにおけるストロミュールの新規機能
藤原誠、吉岡泰、風間裕介、平野智也、丹羽康夫、森山崇、佐藤直樹、阿部知子、吉田茂男、伊藤竜一
日本農芸化学会2026年度大会
Event date： 2026.3.9 - 2026.3.12
Language：Japanese Presentation type：Oral presentation (general)
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奄美群島に生息するナワシロイチゴの高頻度三倍体発生原因の解明
河本大輝，登島早紀，平野智也，國武久登
日本育種学会 第20回九州育種談話会 2025.12.7
Event date： 2025.12.7
Language：Japanese Presentation type：Poster presentation
Awards 【 display / non-display 】
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日本育種学会賞
2023.3 日本育種学会 重イオンビームによる育種技術の開発
重イオン育種技術開発グループ（阿部知子、風間裕介、平野智也）
Award type：International academic award (Japan or overseas)
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CYTOLOGIA奨励賞
2025.6 日本メンデル協会 重イオンビーム照射によるDNA損傷と重複受精の関連性の解明
平野智也
Award type：International academic award (Japan or overseas)
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宮崎銀行ふるさと振興助成事業
2025.3 みやぎん経済研究所
平野智也
Award type：Award from publisher, newspaper, foundation, etc.
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RIKEN Accelerator Progress Report 2023 Vol. 57 HIGHLIGHTS OF THE YEAR
2024.12 理化学研究所仁科加速器研究センター加速器年次報告編集委員会 Characterization of a novel mutant with inhibition of storage root formation in sweet potato
H. Park, T. Abe, H. Kunitake, T. Hirano
Award type：Honored in official journal of a scientific society, scientific journal Country：Japan
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平成28年度園芸学会年間優秀論文賞
2017.3 園芸学会
澤田 倫平, 平野 智也, 飯牟禮 和彦, 阿部 知子, 尾崎 行生
Award type：Honored in official journal of a scientific society, scientific journal Country：Japan
Grant-in-Aid for Scientific Research 【 display / non-display 】
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種子発達を制御する重複受精過程のDNA損傷応答に関する研究
Grant number：25K09094 2025.04 - 2028.03
独立行政法人日本学術振興会 科学研究費基金 基盤研究(C)
Authorship：Principal investigator
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人為誘発非還元性雄性配偶子を活用した倍数性多様化法の開発
Grant number：20K06035 2020.04 - 2024.03
独立行政法人日本学術振興会 科学研究費補助金 基盤研究(C)
Authorship：Principal investigator
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染色体微細加工で逆位・転座が植物ゲノムに与える影響を見る
Grant number：20H03297 2020.04 - 2023.03
独立行政法人日本学術振興会 科学研究費補助金 基盤研究(B)
Authorship：Coinvestigator(s)
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Establishment of analysis method for double fertilization process using unreduced male gamete
Grant number：17K15223 2017.04 - 2020.03
Grant-in-Aid for Scientific Research Grant-in-Aid for Young Scientists(B)
Authorship：Principal investigator
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高等植物における異型精細胞形成機構の解明
Grant number：22780034 2010.04 - 2013.03
科学研究費補助金 若手研究(B)
Authorship：Principal investigator
キルタンサスでは、花粉管伸長過程で一組の精細胞が異型化し、雄原細胞分裂直後の表層微小管構成時に蓄積の差異が生じる事が明らかとなった。精細胞形成の分子基盤情報を得るために遺伝子解析を行い、異型化特異的ステージの前後で発現量が変化する遺伝子の候補リストが得られた。花粉に重イオンビーム照射することで、雄性配偶子の研究に有用な新たな材料を作出するとともに、雄性配偶子のDNA損傷応答機構を明らかにした。
Other research activities 【 display / non-display 】
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Chrysanthemum x morifolium Ramat. ‘長崎SYC1’
2021.03
品種登録 出願番号35321
育成者：久村麻子、諸岡淳司、阿部知子、林依子、平野智也、石井公太郎 -
Chrysanthemum x morifolium Ramat. ‘長崎SWC1’
2019.03
品種登録 登録番号28537
育成者：久村麻子、諸岡淳司、阿部知子、林依子、平野智也 -
Prunus L. '仁科知花'
2016.06
品種登録 出願番号31199
育成者：石井重久、阿部知子、林 依子、平野智也 -
Chrysanthemum x morifolium Ramat. ‘白涼’
2015.03
品種登録 登録番号26306
育成者：峯大樹、岳田司、久村麻子、阿部知子、平野智也、林依子 -
Prunus L. ‘仁科小町’
2012.09 - 2022.06
品種登録 出願番号27386
育成者：石井重久、阿部知子、林依子、福西暢尚、平野智也
Available Technology 【 display / non-display 】
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微細操作技術を活用した植物の受精機構に関する研究
突然変異育種法の効率化に関する研究
ラン科植物の長期遺伝資源保存法の開発Home Page： 植物遺伝育種学研究室
Related fields where technical consultation is available：・花き植物を中心とした突然変異育種
・植物遺伝資源の保存、植物組織培養による増殖
・突然変異遺伝子の同定、単離Message：・共同研究の希望テーマ：花き園芸植物の品種改良
・少数細胞解析技術の開発ニーズがあれば、ぜひご連絡ください。