HIRANO Tomonari

写真a

Affiliation

Faculty of Agriculture Department of Biochemistry and Applied Biosciences

Title

Associate Professor

External Link

Degree 【 display / non-display

  • Doctor (Agriculture) ( 2006.3   Chiba University )

  • 修士(農学) ( 2003.3   千葉大学 )

  • 学士(農学) ( 2001.3   千葉大学 )

Research Interests 【 display / non-display

  • Mutation

  • DNA double strand break

  • Flower development

  • Double fertilization

  • Male gamete

Research Areas 【 display / non-display

  • Environmental Science/Agriculture Science / Horticultural science

  • Environmental Science/Agriculture Science / Science in plant genetics and breeding

 

Papers 【 display / non-display

  • Distinctive development of embryo and endosperm caused by male gametes irradiated with carbon-ion beam Reviewed

    Tomonari Hirano, Muneaki Murata, Yurie Watarikawa, Yoichiro Hoshino, Tomoko Abe, Hisato Kunitake

    Plant Reproduction   2024.4

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    Authorship:Lead author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:Plant Reproduction  

    Key message: In Cyrtanthus mackenii, development of embryo and endosperm were differentially affected by fertilization of male gametes with DNA damage and mutations. Abstract: Pollen irradiation with ionizing radiations has been applied in plant breeding and genetic research, and haploid plant induction has mainly been performed by male inactivation with high-dose irradiation. However, the fertilization process of irradiated male gametes and the early development of embryo and endosperm have not received much attention. Heavy-ion beams, a type of radiation, have been widely applied as effective mutagens for plants and show a high mutation rate even at low-dose irradiation. In this study, we analyzed the effects of male gametes of Cyrtanthus mackenii irradiated with a carbon-ion beam at low doses on fertilization. In immature seeds derived from the pollination of irradiated pollen grains, two types of embryo sacs were observed: embryo sac with a normally developed embryo and endosperm and embryo sac with an egg cell or an undivided zygote and an endosperm. Abnormalities in chromosome segregation, such as chromosomal bridges, were observed only in the endosperm nuclei, irrespective of the presence or absence of embryogenesis. Therefore, in Cyrtanthus, embryogenesis is strongly affected by DNA damage or mutations in male gametes. Moreover, various DNA contents were detected in the embryo and endosperm nuclei, and endoreduplication may have occurred in the endosperm nuclei. As carbon-ion irradiation causes chromosomal rearrangements even at low doses, pollen irradiation can be an interesting tool for studying double fertilization and mutation heritability.

    DOI: 10.1007/s00497-024-00496-9

    Scopus

    PubMed

  • Characterization of a novel mutant with inhibition of storage root formation in sweet potato Reviewed

    Hyungjun Park, Tomoko Abe, Hisato Kunitake, Tomonari Hirano

    Breeding Science   73 ( 2 )   212 - 218   2023.6

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    Authorship:Last author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:日本育種学会  

    Sweet potato is a widely cultivated crop with storage roots. Although many studies have been conducted on the mechanism of its storage root formation, the details have not been fully elucidated. We screened mutant lines with inhibition of storage root formation to clarify parts of the mechanism. In this study, the process of storage root formation in one of the mutant lines, C20-8-1, was investigated. The inhibition of storage root formation was observed during the early stages of growth. The roots in C20-8-1 did not show histological differences compared to those in wild type. The transition from fibrous roots to pencil roots, which are the developmental stages prior to mature storage root formation, was delayed or inhibited in C20-8-1. The upregulation of starch biosynthesis-related genes and downregulation of lignin biosynthesis genes with storage root swelling were not confirmed in the root of C20-8-1 during the developmental transition stage, suggesting that most of the roots in C20-8-1 are in the pre-transition state toward the storage root swelling. C20-8-1 showed a mutant phenotype during the critical period of storage root swelling initiation, and further clarification of this mutation is expected to provide new insights into storage root formation.

    DOI: 10.1270/jsbbs.22090

    CiNii Research

  • Effect of Growth Stages on Anthocyanins and Polyphenols in the Root System of Sweet Potato. Reviewed

    Setoguchi Y, Nakagawa S, Ohmura R, Toshima S, Park H, Narasako Y, Hirano T, Otani M, Kunitake H

    Plants (Basel, Switzerland)   12 ( 9 )   2023.5

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.3390/plants12091907

    PubMed

  • Amphidiploid production of hybrid between black raspberry and Rubus parvifolius L., a wild Asian species, using colchicine treatment Reviewed

    Saki Toshima, Itsuki Katsumi, Arisa Kai, Masaki Yahata, Tomonari Hirano, Hisato Kunitake

    Scientia Horticulturae   312   111863   2023.3

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: https://doi.org/10.1016/j.scienta.2023.111863.

  • Comparison of Proanthocyanidin Content in Rabbiteye Blueberry (Vaccinium virgatum Aiton) Leaves and the Promotion of Apoptosis against HL-60 Promyelocytic Leukemia Cells Using ‘Kunisato 35 Gou’ Leaf Extract Reviewed

    Toyama, Yuki, Yoko Fujita, Saki Toshima, Tomonari Hirano, Masao Yamasaki, Hisato Kunitake

    Plants   12 ( 4 )   948   2023.2

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: https://doi.org/10.3390/plants12040948

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Books 【 display / non-display

  • エッセンシャル植物育種学 : 農学系のための基礎

    國武 久登 , 執行 正義, 平野 智也, 神戸 敏成, 貴島 祐治 , 三吉 一光, 大谷 基泰, 八幡 昌紀, 星野 洋一郎, 中野 優 , 佐々 英徳, 三柴 啓一郎, 山本 伸一 (農学)( Role: Joint editor)

    講談社  2023.5  ( ISBN:9784065308158

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    Language:Japanese Book type:Textbook, survey, introduction

    CiNii Books

  • 最新農業技術 花卉〈vol.5〉切り花で新境地をひらくアジサイ、ダリア、ラナンキュラス、ユーストマ

    阿部 知子, 平野 智也( Role: Joint author ,  突然変異(イオンビーム)育種)

    農山漁村文化協会  2013.3 

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    Language:Japanese Book type:Scholarly book

  • Pollen: Structure, Types and Effects

    Tomonari Hirano, Yoichiro Hoshino( Role: Joint author ,  Capture of male gamete dynamics in pollen tubes)

    Nova Science Publishers  2010.7 

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    Language:English Book type:Scholarly book

  • 日本の植物園における生物多様性保全

    平野 智也, 神戸 敏成( Role: Joint author ,  日本産希少植物の繁殖および長期保存)

    日本植物園協会・国立博物館筑波実験植物園・植物園自然保護国際機構  2007.3 

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    Language:Japanese Book type:Scholarly book

  • Floriculture, Ornamental and Plant Biotechnology: advances and topical issues

    Tomonari Hirano, Keiko Ishikawa, Masahiro Mii( Role: Joint author ,  Advances in Orchid Cryopreservation)

    Global Science Book  2006.6 

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    Language:English Book type:Scholarly book

MISC 【 display / non-display

  • Development of breeding technology by using heavy-ion beam Invited Reviewed

    Ion-Beam Breeding Research Group, Abe Tomoko, Kazama Yusuke, Hirano Tomonari

    Breeding Research   25 ( 2 )   170 - 176   2023.12

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    Authorship:Last author   Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (scientific journal)   Publisher:Japanese Society of Breeding  

    DOI: 10.1270/jsbbr.23j13

    CiNii Research

  • イオンビームを用いた花き植物の品種改良 Invited Reviewed

    平野智也

    アグリバイオ   6 ( 12 )   14 - 18   2022.10

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    Authorship:Lead author, Corresponding author   Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media)  

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  • Mutagenic Effects of Heavy-Ion Beam Irradiation to Plant Genome Reviewed

    Tomonari Hirano, Yusuke Kazama, Hisato Kunitake, Tomoko Abe

    CYTOLOGIA   87 ( 1 )   3 - 6   2022.3

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    Authorship:Lead author, Corresponding author   Language:English   Publishing type:Article, review, commentary, editorial, etc. (scientific journal)  

  • Chromosomal rearrangement: From induction by heavy-ion irradiation to in vivo engineering by genome editing Reviewed

    Yusuke Kazama, Tomonari Hirano, Tomoko Abe, Sachihiro Matsunaga

    Cytologia   83 ( 2 )   125 - 128   2018.6

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    Language:English   Publishing type:Article, review, commentary, editorial, etc. (scientific journal)  

  • 重イオンビームで広がる花きの新品種作出 Invited Reviewed

    平野 智也,市田 裕之,阿部 知子

    化学と生物   55 ( 11 )   775 - 782   2017.10

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    Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (scientific journal)  

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Presentations 【 display / non-display

  • サツマイモ基腐病抵抗性品種選抜のためのポリフェノールマーカーの開発

    瀬戸口優乃、福留春香、白石麗依奈、奈良迫洋介、竹下稔、 大谷基泰、平野智也、國武久登

    日本作物学会第257回講演会  2024.3.28 

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    Event date: 2024.3.28 - 2024.3.29

    Language:Japanese   Presentation type:Poster presentation  

  • オオカナダモの異型細胞形成の研究:細胞小器官の構造について

    藤原誠、小林永実、河野杏奈、金澤美加子、平野智也、阿部知子、伊藤竜一

    日本生物教育学会第108回全国大会 

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    Event date: 2024.1.6 - 2024.1.8

    Language:Japanese   Presentation type:Oral presentation (general)  

  • Phylogenetic classification of a Japanese raspberry wild species, Rubus parvifolius International conference

    Saki Toshima, Yuichi Saeki, Tomonari Hirano, Hisato Kunitake

    The 4th Asian Horticultural Congress (AHC2023)  2023.9.29 

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    Event date: 2023.9.29 - 2023.9.30

    Language:English   Presentation type:Oral presentation (general)  

  • 大学発サツマイモ育種の試み

    瀬戸口優乃、奈良迫洋介、大谷基泰、平野智也、國武久登

    第23回 分析講演会  2023.9.22 

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    Event date: 2023.9.22

    Language:Japanese   Presentation type:Poster presentation  

  • Effects of fertilization of male gametes with argon-ion irradiation on embryo and endosperm development in Cyrtanthus mackenii International conference

    Makiko Shii, Yuki Kajiya Tomoko Abe, Hisato Kunitake, Tomonari Hirano

    15th JKTC International Student Seminar  2023.9.19 

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    Event date: 2023.9.18 - 2023.9.21

    Language:English   Presentation type:Oral presentation (general)  

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Awards 【 display / non-display

  • 日本育種学会賞

    2023.3   日本育種学会   重イオンビームによる育種技術の開発

    重イオン育種技術開発グループ(阿部知子、風間裕介、平野智也)

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    Award type:Award from Japanese society, conference, symposium, etc. 

  • 平成28年度園芸学会年間優秀論文賞

    2017.3   園芸学会  

    澤田 倫平, 平野 智也, 飯牟禮 和彦, 阿部 知子, 尾崎 行生

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    Award type:Honored in official journal of a scientific society, scientific journal  Country:Japan

  • RIKEN Accelerator Progress Report 2015 Vol. 49 HIGHLIGHTS OF THE YEAR

    2017.3   仁科加速器研究センター加速器年次報告編集委員会  

    A. Hisamura, D. Mine, T. Takeda, T. Abe, Y. Hayashi, T. Hirano

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    Award type:Honored in official journal of a scientific society, scientific journal  Country:Japan

  • RIKEN Accelerator Progress Report 2015 Vol. 49 HIGHLIGHTS OF THE YEAR

    2017.3   仁科加速器研究センター加速器年次報告編集委員会  

    T. Hirano, Y. Kazama, K. Ishii, S. Ohbu, Y. Shirakawa, T. Abe

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    Award type:Honored in official journal of a scientific society, scientific journal  Country:Japan

  • 第125回講演会日本育種学会優秀発表賞 (講演番号P28)

    2014.5   日本育種学会  

    平野 智也, 風間 裕介, 石井 公太郎, 大部 澄江, 白川 侑希, 阿部 知子

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    Award type:Award from Japanese society, conference, symposium, etc.  Country:Japan

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Grant-in-Aid for Scientific Research 【 display / non-display

  • 人為誘発非還元性雄性配偶子を活用した倍数性多様化法の開発

    Grant number:20K06035  2020.04 - 2024.03

    独立行政法人日本学術振興会  科学研究費補助金  基盤研究(C)

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    Authorship:Principal investigator 

  • 染色体微細加工で逆位・転座が植物ゲノムに与える影響を見る

    Grant number:20H03297  2020.04 - 2023.03

    独立行政法人日本学術振興会  科学研究費補助金  基盤研究(B)

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    Authorship:Coinvestigator(s) 

  • Establishment of analysis method for double fertilization process using unreduced male gamete

    2017.04 - 2020.03

    Grant-in-Aid for Scientific Research  Grant-in-Aid for Young Scientists(B)

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    Authorship:Principal investigator 

  • 高等植物における異型精細胞形成機構の解明

    2010.04 - 2013.03

    科学研究費補助金  若手研究(B)

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    Authorship:Principal investigator 

    キルタンサスでは、花粉管伸長過程で一組の精細胞が異型化し、雄原細胞分裂直後の表層微小管構成時に蓄積の差異が生じる事が明らかとなった。精細胞形成の分子基盤情報を得るために遺伝子解析を行い、異型化特異的ステージの前後で発現量が変化する遺伝子の候補リストが得られた。花粉に重イオンビーム照射することで、雄性配偶子の研究に有用な新たな材料を作出するとともに、雄性配偶子のDNA損傷応答機構を明らかにした。

Other research activities 【 display / non-display

  • Chrysanthemum x morifolium Ramat. ‘長崎SYC1’

    2021.03

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    品種登録 出願番号35321

    育成者:久村麻子、諸岡淳司、阿部知子、林依子、平野智也、石井公太郎

  • Chrysanthemum x morifolium Ramat. ‘長崎SWC1’

    2019.03

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    品種登録 登録番号28537

    育成者:久村麻子、諸岡淳司、阿部知子、林依子、平野智也

  • Prunus L. '仁科知花'

    2016.06

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    品種登録 出願番号31199

    育成者:石井重久、阿部知子、林 依子、平野智也

  • Chrysanthemum x morifolium Ramat. ‘白涼’

    2015.03

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    品種登録 登録番号26306

    育成者:峯大樹、岳田司、久村麻子、阿部知子、平野智也、林依子

  • Prunus L. ‘仁科小町’

    2012.09

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    品種登録 出願番号27386

    育成者:石井重久、阿部知子、林依子、福西暢尚、平野智也

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