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Faculty of Medicine College Hospital Department of Pathology |
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Assistant Professor |
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Related SDGs |
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MAEKAWA Kazunari
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Papers 【 display / non-display 】
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Gi T, Kuwahara A, Yamashita A, Matsuda S, Maekawa K, Moriguchi-Goto S, Sato Y, Asada Y
Arteriosclerosis, thrombosis, and vascular biology 43 ( 1 ) 146 - 159 2022.11
Language：English Publishing type：Research paper (scientific journal) Publisher：Arteriosclerosis, Thrombosis, and Vascular Biology
Background: Cancer-associated venous thromboembolism (VTE) is a critical complication in patients with cancer. However, the pathological findings of VTE are limited. Here, we investigated the histopathological features of cancer-associated VTE in human autopsy cases. Methods: We clinically examined the autopsy cases of VTE with (n=114) and without cancer (n=66) and immunohistochemically analyzed the expression of prothrombotic factors in intrathrombus cancer cells, the thrombus contents of erythrocytes, fibrin, platelets, citrullinated histone H3, and degree of organization. Results: Vascular wall invasion or small cell clusters of cancer cells was observed in thrombi in 27.5% of deep vein thrombosis and 25.9% of pulmonary embolism cases. The majority of the cancer cells in deep vein thrombi appeared to be invading the vessel wall, whereas the majority of pulmonary thrombi had cancer cell clusters, consistent with embolization via blood flow. These cancer cells were immunohistochemically positive for TF (tissue factors) or podoplanin in up to 88% of VTE cases. The frequency of TF-positive monocyte/macrophages in thrombi was higher in cancer-associated VTE than that in VTE without cancer. Citrullinated histone H3 was predominantly observed in the early stages of organizing thrombi. There was no significant difference in thrombus components between VTE with cancer and without cancer groups. Conclusions: Vascular wall invasion or cancer cell clusters in thrombi might influence thrombogenesis of cancer-associated VTE. TF and podoplanin in cancer cells and in monocyte/macrophages may induce coagulation reactions and platelet aggregation. Neutrophil extracellular traps may play a role in the early stages of VTE, regardless of cancer status.
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Maekawa K., Tsuji A.B., Yamashita A., Sugyo A., Katoh C., Tang M., Nishihira K., Shibata Y., Koshimoto C., Zhang M.R., Nishii R., Yoshinaga K., Asada Y.
Atherosclerosis 337 7 - 17 2021.11
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Atherosclerosis
Background and aims: This study aimed to investigate whether N-benzyl-N-methyl-2-[7,8-dihydro-7-(2-[18F]fluoroethyl)-8-oxo-2-phenyl-9H-purin-9-yl]acetamide (18F-FEDAC), a probe for translocator protein (TSPO), can visualize atherosclerotic lesions in rabbits and whether TSPO is localized in human coronary plaques. Methods: 18F-FEDAC-PET of a rabbit model of atherosclerosis induced by a 0.5% cholesterol diet and balloon injury of the left carotid artery (n = 7) was performed eight weeks after the injury. The autoradiography intensity of 18F-FEDAC in carotid artery tissue sections was measured, and TSPO expression was evaluated immunohistochemically. TSPO expression was examined in human coronary arteries obtained from autopsy cases (n = 16), and in human coronary plaques (n = 12) aspirated from patients with acute myocardial infarction (AMI). Results: 18F-FEDAC-PET visualized the atherosclerotic lesions in rabbits as high-uptake areas, and the standard uptake value was higher in injured arteries (0.574 ± 0.24) than in uninjured arteries (0.277 ± 0.13, p < 0.05) or myocardium (0.189 ± 0.07, p < 0.05). Immunostaining showed more macrophages and more TSPO expression in atherosclerotic lesions than in uninjured arteries. TSPO was localized in macrophages, and arterial autoradiography intensity was positively correlated with macrophage concentration (r = 0.64) and TSPO (r = 0.67). TSPO expression in human coronary arteries was higher in AMI cases than in non-cardiac death, or in the vulnerable plaques than in early or stable lesions, respectively. TSPO was localized in macrophages in all aspirated coronary plaques with thrombi. Conclusions: 18F-FEDAC-PET can visualize atherosclerotic lesions, and TSPO-expression may be a marker of high-risk coronary plaques.
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Glioblastoma mimicking metastatic small cell carcinoma: A case report with ultrastructural findings. Reviewed International journal
Kazunari Maekawa, Takako Tokumitsu, Hiroshi Noguchi, Eriko Nakamura, Toshihiro Gi, Shoichi Horinouchi, Shinji Yamashita, Hideo Takeshima, Yujiro Asada, Yuichiro Sato
Diagnostic cytopathology 49 ( 8 ) E291 - E296 2021.8
Authorship：Lead author Language：Japanese Publishing type：Research paper (scientific journal)
It is often straightforward to distinguish glioblastoma (GBM) from metastatic carcinoma by cytology; however, small cell variants of GBM or GBM with primitive neuronal component (GBMPNC) can mimic metastatic small cell carcinoma (SCC). Herein, we report a case of GBMPNC mimicking metastatic SCC and present cytological and ultrastructural findings. A 65-year-old man with memory disturbance was hospitalized. Magnetic resonance imaging revealed the presence of a 6 cm sized tumor in the right anterior temporal lobe. Intraoperative cytology slides indicated that the tumor consisted of small-sized cells with scant cytoplasm showing high cellularity. The initial intraoperative diagnosis was metastatic SCC; however, any primary visceral tumor was not detected clinically. Immunohistochemical and ultrastructural studies of postoperative histological sections revealed that the lesion was GBMPNC. This case shows that some GBMs may have the potential to closely mimic metastatic SCC, which expands the differential diagnosis and emphasizes the importance of clinical correlation.
DOI： 10.1002/dc.24715
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Characterization of Carotid Plaque Components by Quantitative Susceptibility Mapping Reviewed
Minako Azuma, Kazunari Maekawa, Atsushi Yamashita, Kiyotaka Yokogami, Masahiro Enzaki, Z.A. Khant, Hideo Takeshima, Yujiro Asada, Y. Wang and Toshinori Hirai
American Journal of Neuroradiology 41 ( 2 ) 310 - 317 2019.12
Language：English Publishing type：Research paper (scientific journal) Publisher：American Journal of Neuroradiology
BACKGROUND AND PURPOSE: Intraplaque hemorrhage in the carotid artery is related to an increased risk of cerebrovascular ischemic events. We aimed to investigate whether quantitative susceptibility mapping can characterize carotid artery plaque components and quantify the severity of intraplaque hemorrhage.
MATERIALS AND METHODS: For this ex vivo quantitative susceptibility mapping study, 9 carotid endarterectomy specimens were
imaged on a 3T MR imaging scanner using a 3D multi-echo gradient-echo sequence and a microscopy coil. The samples were examined histologically using immunostains, including glycophorin A and Prussian blue. The areas of erythrocytes, iron deposits, calcification, and fibrous matrices observed on stained sections were compared with quantitative susceptibility mapping findings and their
mean susceptibility values.
RESULTS: Intraplaque hemorrhage and iron deposits were observed only in areas hyperintense on quantitative susceptibility mapping; calcifications and fibrous matrices were prevalent in hypointense areas. The mean susceptibility values for necrotic cores with
intraplaque hemorrhage but no iron deposits, cores with iron deposits but no intraplaque hemorrhage, cores without either intraplaque hemorrhage or iron deposits, and cores with calcification were 188 6 51, 129 6 49, 11 6 17, and 158 6 78 parts per billion, respectively. There was a significant difference in the mean susceptibility values among the 4 histologic components (P , .01).
The mean susceptibility values of the whole plaque positively correlated with the percentage area positive for glycophorin A
(r=0.65, P<0.001) and Prussian blue (r=0.47, P<0.001).
CONCLUSIONS: Our findings suggest that quantitative susceptibility mapping can characterize the composition of carotid plaques
and quantify the degree of intraplaque hemorrhage and iron deposits.DOI： 10.3174/ajnr.A6374
Other Link： https://www.ncbi.nlm.nih.gov/pubmed/31879331
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Nakamura E, Sato Y, Iwakiri T, Yamashita A, Moriguchi-Goto S, Maekawa K, Gi T, Asada Y
Journal of atherosclerosis and thrombosis 2017.9
Language：English Publishing type：Research paper (scientific journal) Publisher：一般社団法人 日本動脈硬化学会
<b><i>Aim</i></b>: Patients with peripheral artery disease (PAD) have a high prevalence of cardiovascular morbidity and mortality; however, majority of patients with PAD are asymptomatic. This study aimed to histologically evaluate whether asymptomatic, lower extremity artery plaques are associated with systemic atherosclerosis and the onset of cardiovascular disease (CVD) events using autopsy cases.<b><i>Methods</i></b>: We histologically investigated the atherosclerotic plaques of the common iliac, common carotid, coronary, and renal arteries from 121 autopsy cases without symptoms of PAD (mean age:67.6 years; 63% men; 83% non-CVD death). We evaluated the relationship between the degree of iliac artery atherosclerosis and that of other arteries, and also the presence of any CVD, myocardial infarction, stroke, and renal failure.<b><i>Results</i></b>: Advanced atherosclerotic plaques (American Heart Association ≥4) were present in 86 (72%) common iliac arteries in these cases. These arteries also showed high frequencies of calcification (66%), intraplaque hemorrhage (42%), and plaque disruption (24%). These advanced lesions were associated with age (≥60 years), sex (male), hypertension, diabetes, and smoking habit (all <i>P</i><b>＜</b>0.05). Additionally, it was significantly associated with CVD (odds ratio, 95% confidence interval; 6.2, 2.2–22), myocardial infarction (6.4, 1.2–19), stroke (8.7, 1.7–16), and renal failure/hemodialysis (5.8, 1.1–11). Cases with advanced iliac artery plaques had advanced coronary and carotid atherosclerosis.<b><i>Conclusion</i></b>: These results indicate that asymptomatic advanced plaques are frequently observed in common iliac arteries, and are associated with generalized atherosclerosis and CVD events.
DOI： 10.5551/jat.39669
Books 【 display / non-display 】
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New Textbook of Clinical Phlebology
Kazunari Maekawa, Yujiro Asada（ Role： Joint author , Anatomy of Vein, Special Vein (intestinal vein and portal vein)）
MEDICAL VIEW 2019.10
Total pages：503 Responsible for pages：12-13 Language：Japanese Book type：Scholarly book
Presentations 【 display / non-display 】
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Translocator proteinは冠動脈不安定プラーク内マクロファージに局在し、18F-FEDAC-PETは家兎動脈硬化病変を描出する
前川和也1、辻 厚至2、山下 篤1、須堯 綾2、西平賢作3、柴田剛徳3、張 明栄4、西井龍一2、浅田祐士郎1
第54回日本動脈硬化学会学術集会
Event date： 2022.7.23 - 2022.7.24
Language：Japanese Presentation type：Poster presentation
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炎症と低酸素環境は冠動脈プラークの血栓形成能を相加的に亢進させる
前川和也1、山下 篤1、松浦祐之介2、西平賢作3、中村恵理子1、西東洋一4、菰原義弘5、畠山金太6、海北幸一2、柴田剛徳3、浅田祐士郎1
第44回日本血栓止血学会学術集会
Event date： 2022.6.23 - 2022.6.25
Language：Japanese Presentation type：Oral presentation (general)
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ハイリスク冠動脈のTranslocator protein発現と18F-FEDAC-PETによる検出
前川和也1、辻 厚至2、山下 篤1、須堯 綾2、西平賢作3、柴田剛徳3、張 明栄4、西井龍一2、浅田祐士郎1
第111回日本病理学会総会
Event date： 2022.4.14 - 2022.4.16
Language：English Presentation type：Poster presentation
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グルタミンは血管平滑筋細胞の凝固活性に関与する
山下 篤 1), 小山彰平 2), 松浦祐之介 2), 前川和也 1), 海北幸一 2), 浅田祐士郎 1)
第26回日本血管病理研究会
Event date： 2021.11.27
Language：Japanese Presentation type：Oral presentation (general)
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Translocator proteinを標的とした、ハイリスク冠動脈プラークの検出
前川和也1、辻 厚2、山下 篤1、須堯 綾2、加藤千恵次3、Minghui Tang3、西平賢作4、柴田剛徳4、越本知大5、Ming-Rong Zhang2、西井龍一2、吉永恵一郎2、浅田祐士郎1
第43回日本血栓止血学会学術集会
Event date： 2021.5.27 - 2021.5.29
Language：Japanese Presentation type：Oral presentation (general)
Awards 【 display / non-display 】
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優秀ポスター賞
2022.6 日本血栓止血学会
前川和也
Award type：Award from Japanese society, conference, symposium, etc.
Grant-in-Aid for Scientific Research 【 display / non-display 】
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Synthetic MRIによる脳腫瘍の定量的評価：真の性状や拡がりを捉える手法の確立
Grant number：24K10787 2024.04 - 2027.03
独立行政法人日本学術振興会 科学研究費基金 基盤研究(C)
Authorship：Coinvestigator(s)
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冠動脈プラークの不安定化におけるビリルビンの関与と非侵襲的検出
Grant number：24K18404 2024.04 - 2027.03
独立行政法人日本学術振興会 科学研究費基金 若手研究
Authorship：Principal investigator
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肺血栓塞栓症における非塞栓部肺動脈の変化と静脈血栓由来因子の解明
Grant number：23K06467 2023.04 - 2026.03
独立行政法人日本学術振興会 科学研究費基金 基盤研究（C）
山下 篤
Authorship：Coinvestigator(s)