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医学部 医学科 病理学講座構造機能病態学分野 |
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Gi T, Kuwahara A, Yamashita A, Matsuda S, Maekawa K, Moriguchi-Goto S, Sato Y, Asada Y
Arteriosclerosis, thrombosis, and vascular biology 43 ( 1 ) 146 - 159 2022年11月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Arteriosclerosis, Thrombosis, and Vascular Biology
Background: Cancer-associated venous thromboembolism (VTE) is a critical complication in patients with cancer. However, the pathological findings of VTE are limited. Here, we investigated the histopathological features of cancer-associated VTE in human autopsy cases. Methods: We clinically examined the autopsy cases of VTE with (n=114) and without cancer (n=66) and immunohistochemically analyzed the expression of prothrombotic factors in intrathrombus cancer cells, the thrombus contents of erythrocytes, fibrin, platelets, citrullinated histone H3, and degree of organization. Results: Vascular wall invasion or small cell clusters of cancer cells was observed in thrombi in 27.5% of deep vein thrombosis and 25.9% of pulmonary embolism cases. The majority of the cancer cells in deep vein thrombi appeared to be invading the vessel wall, whereas the majority of pulmonary thrombi had cancer cell clusters, consistent with embolization via blood flow. These cancer cells were immunohistochemically positive for TF (tissue factors) or podoplanin in up to 88% of VTE cases. The frequency of TF-positive monocyte/macrophages in thrombi was higher in cancer-associated VTE than that in VTE without cancer. Citrullinated histone H3 was predominantly observed in the early stages of organizing thrombi. There was no significant difference in thrombus components between VTE with cancer and without cancer groups. Conclusions: Vascular wall invasion or cancer cell clusters in thrombi might influence thrombogenesis of cancer-associated VTE. TF and podoplanin in cancer cells and in monocyte/macrophages may induce coagulation reactions and platelet aggregation. Neutrophil extracellular traps may play a role in the early stages of VTE, regardless of cancer status.
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Maekawa K., Tsuji A.B., Yamashita A., Sugyo A., Katoh C., Tang M., Nishihira K., Shibata Y., Koshimoto C., Zhang M.R., Nishii R., Yoshinaga K., Asada Y.
Atherosclerosis 337 7 - 17 2021年11月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Atherosclerosis
Background and aims: This study aimed to investigate whether N-benzyl-N-methyl-2-[7,8-dihydro-7-(2-[18F]fluoroethyl)-8-oxo-2-phenyl-9H-purin-9-yl]acetamide (18F-FEDAC), a probe for translocator protein (TSPO), can visualize atherosclerotic lesions in rabbits and whether TSPO is localized in human coronary plaques. Methods: 18F-FEDAC-PET of a rabbit model of atherosclerosis induced by a 0.5% cholesterol diet and balloon injury of the left carotid artery (n = 7) was performed eight weeks after the injury. The autoradiography intensity of 18F-FEDAC in carotid artery tissue sections was measured, and TSPO expression was evaluated immunohistochemically. TSPO expression was examined in human coronary arteries obtained from autopsy cases (n = 16), and in human coronary plaques (n = 12) aspirated from patients with acute myocardial infarction (AMI). Results: 18F-FEDAC-PET visualized the atherosclerotic lesions in rabbits as high-uptake areas, and the standard uptake value was higher in injured arteries (0.574 ± 0.24) than in uninjured arteries (0.277 ± 0.13, p < 0.05) or myocardium (0.189 ± 0.07, p < 0.05). Immunostaining showed more macrophages and more TSPO expression in atherosclerotic lesions than in uninjured arteries. TSPO was localized in macrophages, and arterial autoradiography intensity was positively correlated with macrophage concentration (r = 0.64) and TSPO (r = 0.67). TSPO expression in human coronary arteries was higher in AMI cases than in non-cardiac death, or in the vulnerable plaques than in early or stable lesions, respectively. TSPO was localized in macrophages in all aspirated coronary plaques with thrombi. Conclusions: 18F-FEDAC-PET can visualize atherosclerotic lesions, and TSPO-expression may be a marker of high-risk coronary plaques.
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Glioblastoma mimicking metastatic small cell carcinoma: A case report with ultrastructural findings 査読あり 国際誌
Maekawa K., Tokumitsu T., Noguchi H., Nakamura E., Gi T., Horinouchi S., Yamashita S., Takeshima H., Asada Y., Sato Y.
Diagnostic Cytopathology 49 ( 8 ) E291 - E296 2021年8月
担当区分：筆頭著者 記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Diagnostic Cytopathology
It is often straightforward to distinguish glioblastoma (GBM) from metastatic carcinoma by cytology; however, small cell variants of GBM or GBM with primitive neuronal component (GBMPNC) can mimic metastatic small cell carcinoma (SCC). Herein, we report a case of GBMPNC mimicking metastatic SCC and present cytological and ultrastructural findings. A 65-year-old man with memory disturbance was hospitalized. Magnetic resonance imaging revealed the presence of a 6 cm sized tumor in the right anterior temporal lobe. Intraoperative cytology slides indicated that the tumor consisted of small-sized cells with scant cytoplasm showing high cellularity. The initial intraoperative diagnosis was metastatic SCC; however, any primary visceral tumor was not detected clinically. Immunohistochemical and ultrastructural studies of postoperative histological sections revealed that the lesion was GBMPNC. This case shows that some GBMs may have the potential to closely mimic metastatic SCC, which expands the differential diagnosis and emphasizes the importance of clinical correlation.
DOI： 10.1002/dc.24715
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Characterization of Carotid Plaque Components by Quantitative Susceptibility Mapping 査読あり
Minako Azuma, Kazunari Maekawa, Atsushi Yamashita, Kiyotaka Yokogami, Masahiro Enzaki, Z.A. Khant, Hideo Takeshima, Yujiro Asada, Y. Wang and Toshinori Hirai
American Journal of Neuroradiology 41 ( 2 ) 310 - 317 2019年12月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：American Journal of Neuroradiology
BACKGROUND AND PURPOSE: Intraplaque hemorrhage in the carotid artery is related to an increased risk of cerebrovascular ischemic events. We aimed to investigate whether quantitative susceptibility mapping can characterize carotid artery plaque components and quantify the severity of intraplaque hemorrhage.
MATERIALS AND METHODS: For this ex vivo quantitative susceptibility mapping study, 9 carotid endarterectomy specimens were
imaged on a 3T MR imaging scanner using a 3D multi-echo gradient-echo sequence and a microscopy coil. The samples were examined histologically using immunostains, including glycophorin A and Prussian blue. The areas of erythrocytes, iron deposits, calcification, and fibrous matrices observed on stained sections were compared with quantitative susceptibility mapping findings and their
mean susceptibility values.
RESULTS: Intraplaque hemorrhage and iron deposits were observed only in areas hyperintense on quantitative susceptibility mapping; calcifications and fibrous matrices were prevalent in hypointense areas. The mean susceptibility values for necrotic cores with
intraplaque hemorrhage but no iron deposits, cores with iron deposits but no intraplaque hemorrhage, cores without either intraplaque hemorrhage or iron deposits, and cores with calcification were 188 6 51, 129 6 49, 11 6 17, and 158 6 78 parts per billion, respectively. There was a significant difference in the mean susceptibility values among the 4 histologic components (P , .01).
The mean susceptibility values of the whole plaque positively correlated with the percentage area positive for glycophorin A
(r=0.65, P<0.001) and Prussian blue (r=0.47, P<0.001).
CONCLUSIONS: Our findings suggest that quantitative susceptibility mapping can characterize the composition of carotid plaques
and quantify the degree of intraplaque hemorrhage and iron deposits.DOI： 10.3174/ajnr.A6374
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Nakamura E, Sato Y, Iwakiri T, Yamashita A, Moriguchi-Goto S, Maekawa K, Gi T, Asada Y
Journal of atherosclerosis and thrombosis 2017年9月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：一般社団法人 日本動脈硬化学会
<b><i>Aim</i></b>: Patients with peripheral artery disease (PAD) have a high prevalence of cardiovascular morbidity and mortality; however, majority of patients with PAD are asymptomatic. This study aimed to histologically evaluate whether asymptomatic, lower extremity artery plaques are associated with systemic atherosclerosis and the onset of cardiovascular disease (CVD) events using autopsy cases.<b><i>Methods</i></b>: We histologically investigated the atherosclerotic plaques of the common iliac, common carotid, coronary, and renal arteries from 121 autopsy cases without symptoms of PAD (mean age:67.6 years; 63% men; 83% non-CVD death). We evaluated the relationship between the degree of iliac artery atherosclerosis and that of other arteries, and also the presence of any CVD, myocardial infarction, stroke, and renal failure.<b><i>Results</i></b>: Advanced atherosclerotic plaques (American Heart Association ≥4) were present in 86 (72%) common iliac arteries in these cases. These arteries also showed high frequencies of calcification (66%), intraplaque hemorrhage (42%), and plaque disruption (24%). These advanced lesions were associated with age (≥60 years), sex (male), hypertension, diabetes, and smoking habit (all <i>P</i><b>＜</b>0.05). Additionally, it was significantly associated with CVD (odds ratio, 95% confidence interval; 6.2, 2.2–22), myocardial infarction (6.4, 1.2–19), stroke (8.7, 1.7–16), and renal failure/hemodialysis (5.8, 1.1–11). Cases with advanced iliac artery plaques had advanced coronary and carotid atherosclerosis.<b><i>Conclusion</i></b>: These results indicate that asymptomatic advanced plaques are frequently observed in common iliac arteries, and are associated with generalized atherosclerosis and CVD events.
DOI： 10.5551/jat.39669
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新臨床静脈学
前川和也，浅田祐士郎（ 担当： 共著 , 範囲: A. 静脈の発生と解剖 ④特殊な静脈（腸管静脈や門脈など））
メジカルビュー社 2019年10月
総ページ数：503 担当ページ：12-13 記述言語：日本語 著書種別：学術書
静脈疾患を極める！
講演・口頭発表等 【 表示 ／ 非表示 】
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Translocator proteinは冠動脈不安定プラーク内マクロファージに局在し、18F-FEDAC-PETは家兎動脈硬化病変を描出する
前川和也1、辻 厚至2、山下 篤1、須堯 綾2、西平賢作3、柴田剛徳3、張 明栄4、西井龍一2、浅田祐士郎1
第54回日本動脈硬化学会学術集会
開催年月日： 2022年7月23日 - 2022年7月24日
記述言語：日本語 会議種別：ポスター発表
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炎症と低酸素環境は冠動脈プラークの血栓形成能を相加的に亢進させる
前川和也1、山下 篤1、松浦祐之介2、西平賢作3、中村恵理子1、西東洋一4、菰原義弘5、畠山金太6、海北幸一2、柴田剛徳3、浅田祐士郎1
第44回日本血栓止血学会学術集会
開催年月日： 2022年6月23日 - 2022年6月25日
記述言語：日本語 会議種別：口頭発表（一般）
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ハイリスク冠動脈のTranslocator protein発現と18F-FEDAC-PETによる検出
前川和也1、辻 厚至2、山下 篤1、須堯 綾2、西平賢作3、柴田剛徳3、張 明栄4、西井龍一2、浅田祐士郎1
第111回日本病理学会総会
開催年月日： 2022年4月14日 - 2022年4月16日
記述言語：英語 会議種別：ポスター発表
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グルタミンは血管平滑筋細胞の凝固活性に関与する
山下 篤 1), 小山彰平 2), 松浦祐之介 2), 前川和也 1), 海北幸一 2), 浅田祐士郎 1)
第26回日本血管病理研究会
開催年月日： 2021年11月27日
記述言語：日本語 会議種別：口頭発表（一般）
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Translocator proteinを標的とした、ハイリスク冠動脈プラークの検出
前川和也1、辻 厚2、山下 篤1、須堯 綾2、加藤千恵次3、Minghui Tang3、西平賢作4、柴田剛徳4、越本知大5、Ming-Rong Zhang2、西井龍一2、吉永恵一郎2、浅田祐士郎1
第43回日本血栓止血学会学術集会
開催年月日： 2021年5月27日 - 2021年5月29日
記述言語：日本語 会議種別：口頭発表（一般）