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Affiliation |
Faculty of Agriculture Region of Animal and Plant Biosciences |
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Professor |
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Related SDGs |
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TAKESHITA Minoru
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Research Areas 【 display / non-display 】
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Environmental Science/Agriculture Science / Plant protection science
Papers 【 display / non-display 】
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Narasako, Y., Setoguchi, Y., Fukutome, H., Hirano, T., Otani, M., Takeshita, M., Kunitake, H.
Horticulturae 11 ( 12 ) 1439 2025.11
Language：English Publishing type：Research paper (scientific journal) Publisher：American Society for Horticultural Science
Foot rot (Diaporthe destruens) of sweetpotato generally infects the stem of the plant and then spreads throughout the plant. In Japan, foot rot is prevalent in the main area of sweetpotato production, and there is a pressing need for the development of resistant cultivars. It is generally believed that polyphenols are involved in defense against stress. However, little is known about the relationship between polyphenols and the susceptibility to foot rot in sweetpotato. We thus analyzed the polyphenol of the disease-free healthy stems and investigated their relationship between susceptibility to the disease to establish basic techniques for selecting cultivars resistant to foot rot. Polyphenol was analyzed by the Folin–Ciocalteu method and high-performance liquid chromatography. Resistance tests were conducted using the direct inoculation method. We observed a correlation between the total polyphenol content and the degree of foot rot disease severity. Surprisingly, cultivars with a low stem polyphenol content (‘Tamaakane’) had lower susceptibility to foot rot, whereas the cultivars with high stem polyphenol content (‘Kokei No. 14’) had higher susceptibility to foot rot. In particular, high positive correlations were shown with several polyphenols, including 3,4-dicaffeoylquinic acid and chlorogenic acid. Furthermore, the results of in vitro experiments also suggested that foot rot may metabolize trace amounts of chlorogenic acid. This discovery has potential applications in the selection of resistant cultivars, contributing to the streamlining of disease resistance tests in breeding programs. It could ultimately shorten the breeding period and help overcome foot rot disease in sweetpotato.
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平野 智也, 竹下 稔, 國武 久登
Journal of the American Society for Horticultural Science 150 ( 3 ) 159 - 167 2025.5
Language：English Publishing type：Research paper (scientific journal) Publisher：American Society for Horticultural Science
Foot rot (Diaporthe destruens) of sweetpotato generally infects the stem of the plant and then spreads throughout the plant. In Japan, foot rot is prevalent in the main area of sweetpotato production, and there is a pressing need for the development of resistant cultivars. It is generally believed that polyphenols are involved in defense against stress. However, little is known about the relationship between polyphenols and the susceptibility to foot rot in sweetpotato. We thus analyzed the polyphenol of the disease-free healthy stems and investigated their relationship between susceptibility to the disease to establish basic techniques for selecting cultivars resistant to foot rot. Polyphenol was analyzed by the Folin–Ciocalteu method and high-performance liquid chromatography. Resistance tests were conducted using the direct inoculation method. We observed a correlation between the total polyphenol content and the degree of foot rot disease severity. Surprisingly, cultivars with a low stem polyphenol content (‘Tamaakane’) had lower susceptibility to foot rot, whereas the cultivars with high stem polyphenol content (‘Kokei No. 14’) had higher susceptibility to foot rot. In particular, high positive correlations were shown with several polyphenols, including 3,4-dicaffeoylquinic acid and chlorogenic acid. Furthermore, the results of in vitro experiments also suggested that foot rot may metabolize trace amounts of chlorogenic acid. This discovery has potential applications in the selection of resistant cultivars, contributing to the streamlining of disease resistance tests in breeding programs. It could ultimately shorten the breeding period and help overcome foot rot disease in sweetpotato.
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分光学的手法による甘藷基腐病の早期発見への試み Reviewed
Wang Ziyu、 松崎李空、下沖理紗、井上結子、吉岡宏晃、竹下 稔、宮崎真佐也、興 雄司
レーザー学会研究会報告 588 23 - 27 2024.9
Language：Japanese Publishing type：Research paper (conference, symposium, etc.)
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Ota E., Masuta C., Takeshita M.
Plant Pathology Journal 39 ( 6 ) 592 - 599 2023.12
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：Plant Pathology Journal
A defective RNA3 (D3Yα) of strain Y of cucumber mosaic virus (CMV-Y) was examined on host-specific maintenance, experimental conditions, and a viral factor required for its generation in plants. D3Yα was stably maintained in cucumber but not in tomato plants for 28 days post inoculation (dpi). D3Yα was generated in Nicotiana tabacum or N. benthamiana after prolonged infection in the second and the third passages, but not in plants of N. benthamiana grown at low temperature at 28 dpi or infected with CMV-Y mutant that had the 2b gene deleted. Collectively, we suggest that generation and retention of D3Yα depends on potential host plants and experimental conditions, and that the 2b protein has a role for facilitation of generation of D3Yα.
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Host-specific adaptation drove the coevolution of leek yellow stripe virus and Allium plants Reviewed
Kawakubo S., Kim H., Takeshita M., Masuta C.
Microbiology Spectrum 11 ( 5 ) 2023.10
Language：English Publishing type：Research paper (scientific journal) Publisher：Microbiology Spectrum
Host adaptation plays a crucial role in virus evolution and is a consequence of long-term interactions between virus and host in a complex arms race between host RNA silencing and viral RNA silencing suppressor (RSS) as counterdefense. Leek yellow stripe virus (LYSV), a potyvirus causing yield loss of garlic, infects several species of Allium plants. The unexpected discovery of an interspecific hybrid of garlic, leek, and great-headed (GH) garlic motivated us to explore the host-adaptive evolution of LYSV. Here, using Bayesian phylogenetic comparative methods and a functional assay of viral RSS activity, we show that the evolutionary context of LYSV has been shaped by the host adaptation of the virus during its coevolution with Allium plants. Our phylogenetic analysis revealed that LYSV isolates from leek and their taxonomic relatives (Allium ampeloprasum complex; AAC) formed a distinct monophyletic clade separate from garlic isolates and are likely to be uniquely adapted to AAC. Our comparative studies on viral accumulation indicated that LYSV accumulated at a low level in leek, whereas LYSVs were abundant in other Allium species such as garlic and its relatives. When RSS activity of the viral P1 and HC-Pro of leek LYSV isolate was analyzed, significant synergism in RSS activity between the two proteins was observed in leek but not in other species, suggesting that viral RSS activity may be important for the viral host-specific adaptation. We thus consider that LYSV may have undergone host-specific evolution at least in leek, which must be driven by speciation of its Allium hosts.
Books 【 display / non-display 】
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植物ウイルス学 第2版
鈴木信弘、 山次康幸、高橋英樹、竹下 稔外（ Role： Joint author）
朝倉書店 2026.3
Language：Japanese Book type：Textbook, survey, introduction
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紫外線（UV）照射によるイチゴうどんこ病に 対する抵抗性誘導の評価法開発
太田江美、 竹下 稔、西村文宏、森 充隆、神頭武嗣、田中雅也、細川宗孝、刑部正博、佐藤 衛（ Role： Joint author）
日本植物防疫協会 2021.12
Language：Japanese Book type：Scholarly book
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サツマイモ病害診断・観察簡易マニュアル
小倉李来, 竹下 稔（ Role： Joint author）
国立大学法人宮崎大学農学部植物病理学研究室 2021.2
Language：Japanese Book type：Report
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（株）共立電機製作所・（株）共立電照との共同研究報告書
太田江美、松浦昌平、竹下 稔（ Role： Joint author）
宮崎大学 2020.4
Language：Japanese Book type：Report
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Cucumber mosaic virus
Minoru Takeshita（ Role： Joint author）
APS Press 2018.12
Language：English Book type：Scholarly book
MISC 【 display / non-display 】
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植物抵抗性誘導剤の作用機作について Invited
竹下 稔
（一社）九防協創立５５周年記念講話会要旨集 4 - 23 2025.9
Authorship：Lead author, Corresponding author Language：Japanese Publishing type：Lecture material (seminar, tutorial, course, lecture, etc.)
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植物ウイルス病害診断
菅野善明、⽵下 稔
日本植物病理学会 第19回 植物病害診断教育プログラム テキスト 99 - 114 2025.9
Authorship：Corresponding author Language：Japanese Publishing type：Lecture material (seminar, tutorial, course, lecture, etc.)
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植物成長と防御応答の調節へのサリチル酸配糖化酵素遺伝子の関与 Invited
竹下 稔、小林優大
植物感染生理談話会論文集 ( 56 ) 77 - 86 2022.9
Authorship：Lead author, Corresponding author Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (scientific journal)
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トマトモザイクウイルス感染によるホオズキの生育への影響と乾熱処理種子由来の地下茎導入によるウイルス病害抑制効果 Invited
早日早貴、黒木修一、竹下 稔
植物防疫 76 ( 7 ) 13 - 16 2022.7
Authorship：Last author Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (scientific journal)
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紫外光照射によるイチゴうどんこ病抑制効果と抵抗性遺伝子の解析 Reviewed
太田江美、西村文宏、佐藤 衛、竹下 稔
日本植物病理学会報 2021.1
Language：Japanese Publishing type：Research paper, summary (national, other academic conference)
Presentations 【 display / non-display 】
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植物抵抗性誘導剤の作用機作について Invited
竹下 稔
（一社）九防協創立５５周年記念講話会 2025.9.26
Event date： 2025.9.26
Language：Japanese Presentation type：Public lecture, seminar, tutorial, course, or other speech
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植物ウイルス病害診断 Invited
竹下 稔
日本植物病理学会 第19回 植物病害診断教育プログラム 2025.9.2
Event date： 2025.9.1 - 2025.9.5
Language：Japanese Presentation type：Public lecture, seminar, tutorial, course, or other speech
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生長点培養による主要病原ウイルスフリーサツマイモの作出
⿊⽊美寿、衞藤護⼤、⻘⼭実樹、⼩倉李来、國武久登、⽵下 稔
令和7年度日本植物病理学会九州部会 2025.11.20
Event date： 2025.11.20 - 2025.11.21
Language：Japanese Presentation type：Oral presentation (general)
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分光学的手法を用いたサツマイモ基腐病菌接種塊根における生体反応の可視化
下沖理紗、王 ⼦煜、内⼭桜太、松崎李空、吉岡宏晃、興 雄司、⽵下 稔
令和7年度日本植物病理学会九州部会 2025.11.20
Event date： 2025.11.20 - 2025.11.21
Language：Japanese Presentation type：Oral presentation (general)
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カンキツトリステザウイルス病害の遺伝子診断における試料採取部位の検討
衞藤護⼤、伊⾖元啓介、⼭名宏美、湯地健⼀、⽵下 稔
令和7年度日本植物病理学会九州部会 2025.11.20
Event date： 2025.11.20 - 2025.11.21
Language：Japanese Presentation type：Oral presentation (general)
Awards 【 display / non-display 】
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令和4年度公募型卒業研究テーマ成果発表会ベストポスター賞
2023.3 宮崎県高等教育コンソーシアム 宮崎市内のビロウ並木の天狗巣・黄化・枯死症状の原因究明と対策
青山実樹、安達鉄矢、山本昭洋、佐伯雄一、櫛間義幸、土屋健一、竹下 稔
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平成17年度日本植物病理学会学術奨励賞
2005.3 日本植物病理学会
竹下 稔
Award type：Award from Japanese society, conference, symposium, etc. Country：Japan
Grant-in-Aid for Scientific Research 【 display / non-display 】
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植物の病害抵抗性反応とバイオマス生産の制御に関与する分子機構の解明
Grant number：21J15403 2021.04 - 2023.03
独立行政法人日本学術振興会 科学研究費補助金 特別研究員奨励費
Authorship：Principal investigator
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サリチル酸配糖化酵素を介した植物ウイルス間の病原性相乗作用に関する分子機構の解明
Grant number：18K05655 2020.04 - 2021.03
科学研究費補助金 基盤研究(C)
Authorship：Principal investigator
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サリチル酸配糖化酵素を介した植物ウイルス間の病原性相乗作用に関する分子機構の解明
Grant number：18K05655 2019.04 - 2020.03
科学研究費補助金 基盤研究(C)
Authorship：Principal investigator
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サリチル酸配糖化酵素を介した植物ウイルス間の病原性相乗作用に関する分子機構の解明
Grant number：18K05655 2018.04 - 2019.03
科学研究費補助金 基盤研究(C)
Authorship：Principal investigator
Other research activities 【 display / non-display 】
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Effects of salicylic acid treatment on accumulation level of cucumber mosaic virus and aphids’ settlement on pepper plants
2023.04 - 2024.03
Available Technology 【 display / non-display 】
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植物病害診断
紫外光照射による植物病害低減
抵抗性誘導剤による植物病害低減Message：植物病害診断、紫外光や抵抗性誘導剤を用いた病害軽減に関する共同研究