Presentations -
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モバイルリアルタイムPCR ” PicoGene® PCR1100” を用いた アフリカ豚熱ウイルス検出試薬の検証
Rissar Siringo Ringo, 岡林 環樹
宮崎県養豚研究会 2024.7.23
Event date： 2024.7.23
Presentation type：Oral presentation (general)
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宮崎県におけるSFTS対策の取組 ~小さな繋がりから大きな輪へ~ Invited
岡林 環樹
岐阜大学市民公開講座「ワンヘルスを考える」 2024.3.18
Event date： 2024.3.18
Presentation type：Oral presentation (invited, special)
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身近な致死性人獣共通感染症SFTSについて Invited
岡林 環樹
鹿児島大学感染症制御のためのシンポジウム 2023.12.12
Event date： 2023.12.12
Presentation type：Oral presentation (invited, special)
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SFTS “重症熱性血小板減少症候群”対策に向けた 宮崎ワンヘルスアプローチ Invited
岡林 環樹
新潟県獣医師会「ワンヘルス県民公開講座」 2023.10.28
Event date： 2023.10.28
Presentation type：Oral presentation (invited, special)
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獣医療者に関連する人獣共通感染症 〜SFTS流行地から見えてきた新しい感染リスクとその対策〜
岡林 環樹
富山県獣医師会セミナー 2023.9.14
Event date： 2023.9.14
Presentation type：Oral presentation (invited, special)
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重症熱性血小板減少症候群(SFTS)流行地における宮崎ワンヘルス研究会の取り組み
岡林 環樹
日本獣医学会
Event date： 2023.9.5 - 2023.9.7
Presentation type：Oral presentation (invited, special)
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SFTSってなに？
岡林 環樹
ネコ市ネコ座 2023.8.6
Event date： 2023.8.5 - 2023.8.6
Presentation type：Oral presentation (invited, special)
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SFTS対策における宮崎ワンヘルス研究会の取り組み Invited
岡林 環樹
One World One Health Research Forum 2023.7.1
Event date： 2023.6.30 - 2023.7.1
Presentation type：Oral presentation (invited, special)
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Development of diagnostic system in CADIC Invited International coauthorship International conference
Tamaki Okabayashi
2nd international symposium on Fish Diseases and Seafood Safety 2023.2.10
Event date： 2023.2.10
Language：English Presentation type：Oral presentation (invited, special)
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犬から2頭の猫への重症熱性血小板減少症候群の院内感染事例 Invited International coauthorship International conference
岡林 環樹
令和4年度日本獣医師会獣医学術学会全国大会 2022.11.11
Event date： 2022.11.11 - 2022.11.13
Language：Japanese Presentation type：Oral presentation (invited, special)
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流行地宮崎から見えてきた 新たなSFTSのリスクとその対策
岡林 環樹
SFTSアップデートセミナー2022 迫る危機への準備と対応を学ぶ 〜感染リスクの高い環境下にある自分たちを守るために〜
Event date： 2022.9.28
Language：Japanese Presentation type：Oral presentation (invited, special)
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2010 FMD outbreak in Miyazaki, Japan. Lessons Learned from Miyazaki Invited
Tamaki Okabayashi
FMD: Research, Detection and Control BADAN RISET DAN INOVASI NADIONAL (National Research and Innovation Agency) 2022.5.19
Event date： 2022.5.19
Language：English Presentation type：Oral presentation (invited, special)
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PCV2: PCR検査の再検証
岡林 環樹
ベーリンガーインゲルハイム養豚セミナー2022「PCV2制御のポイント」
Event date： 2022.4.20
Presentation type：Oral presentation (invited, special)
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MOLECULAR MECHANISMS FOR ENHANCEMENT OF BACTERIAL ATTACHMENT TO BOVINE RESPIRATORY EPITHELIAL CELLS BY VIRUS INFECTION International conference
Association of Japan-Indonesia Veterinary Education (Bogor, Indonesia) IPB University
Event date： 2021.2.13
Language：English Presentation type：Oral presentation (general)
Venue：Bogor, Indonesia
Bovine respiratory disease complex (BRDC) is one of the most common and costly diseases in the cattle industry worldwide due to decreased production and increased costs associated with treatment. BRDC known as multiple infections by viruses and bacteria is considered to increase disease severity. Bovine RS virus (BRSV) and bovine coronavirus (BCoV) are the viral-pathogens causing BRDC. Pasteurella multocida (PM) was the most common bacterial agent paired with virus in BRDC.
To investigate the interaction between bovine respiratory viruses and bacteria in bovine respiratory epithelial cells, we generated respiratory epithelial cell lines derived from bovine trachea (bTEC), bronchus (bBEC), and lung (bLEC). These established cells were infected with BRSV or BCoV. After then, we inoculated PM to these viral infected cells. BRSV infection significantly increased PM adherence to bovine lower respiratory tract epithelial cells, but not upper respiratory tract epithelial cells. All the cells infected with BCoV increased adherence of PM. These results indicate that BCoV infection in the upper and lower respiratory tracts enhance adherence of bacteria, and this phenomenon was markedly different from the BRSV infection.
To investigate whether virus infection regulates any cellular adherence receptors on bovine epithelial cells, we performed proteomic and functional analyses. The proteomic analysis showed that BRSV infection decreased expression of intercellular adhesion molecule-1 (ICAM1) on bTECs. However, BRSV infection increased the accumulation of platelet-activating factor receptor (PAFR) in all cell types. Molecular experiments, including specific blockade, knockdown, and overexpression of these receptors, indicated that PM adherence to these cell types depended on PAFR expression. These data suggested that while bTECs possibly captures PM under normal condition, BRSV infection reverses this phenomena.
To examine the interaction between BCoV infection and PM adherence in bovine upper and lower respiratory tract, epithelial cells were infected with BCoV and/or PM. The cells derived from both the upper and lower respiratory tract were susceptible to BCoV infection. These PM adherences are affected by increased expression of ICAM1 and PAFR.
We have obtained evidence suggesting that the upper respiratory tract is responsible for inhibiting PM invasion of the lower respiratory tract. This inhibition is accomplished by trapping PM via ICAM1, an adherence molecule that is displayed on the cellular surface under normal conditions. By capturing microbes, ICAM1 displayed by cells of the upper respiratory tract acts as a “GATEWAY" that impedes bacterial invasion of the lower respiratory tract. BRSV infection causes malfunction of ICAM1 in the bovine upper respiratory tract, allowing bacterial invasion of the lower respiratory tract. BRSV infection increased PAFR expression in epithelial cells derived from the lower respiratory tract. We further demonstrated that PM adherence to cells of the lower respiratory tract was enhanced by upregulation of PAFR. Overall, these findings highlight the importance of the synergistic effect of BRSV infection on severe pneumonia in cattle. BCoV infection enhanced PM adherence and triggered immune responses in cells derived from upper and lower respiratory tracts. BCoV infection up-regulated both ICAM1 and PAFR expressions, enhancing the bacteria adherence to the BCoV-infected cells. The interaction between surface receptors and bacteria adherence in upper respiratory tract may prevent severe pneumonia. In contrast, infection of lower respiratory epithelial cells with BCoV increased PM adherence and stimulated an excess immune response, leading to severe pneumonia. Taken together, the present study presents the evidence for distinct mechanism of BRDC-related infections in response to viral infections in respiratory epithelial cells. Understanding this complex interplay will help us to develop new therapeutic strategies for BRDC.
Thus, we identify a possible molecular mechanism of fundamental difference of pathogenicity in bovine respiratory virus infections in BRDC.