論文 - 岡林 環樹
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Nosocomial Severe Fever with Thrombocytopenia Syndrome in Companion Animals, Japan, 2022 査読あり
Mekata H., Umeki K., Yamada K., Umekita K., Okabayashi T.
Emerging Infectious Diseases 29 ( 3 ) 614 - 617 2023年3月
担当区分：責任著者 記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Emerging Infectious Diseases
In Japan, 2 cats that underwent surgery in a room where a sick dog had been euthanized became ill within 9 days of surgery. Severe fever with thrombocytopenia syndrome virus was detected in all 3 animals; nucleotide sequence identity was 100%. Suspected cause was an uncleaned pulse oximeter probe used for all patients.
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Editorial: Zoonotic diseases among pigs 査読あり
Lin C.N., Okabayashi T., Tummaruk P., Ooi P.T.
Frontiers in Veterinary Science 9 2023年1月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Frontiers in Veterinary Science
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宮崎ワンヘルス研究会の取り組み 招待あり
岡林 環樹
日本獣医師会雑誌 2022年9月
担当区分：筆頭著者, 責任著者 記述言語：日本語 掲載種別：研究論文（学術雑誌）
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Retrospective study on the possibility of an SFTS outbreak associated with undiagnosed febrile illness in veterinary professionals and a family with sick dogs in 2003. 査読あり
Kirino Y, Yamanaka Y, Ishijima K, Tatemae K, Maeda K, Okabayashi T.
Journal of Infection and Chemotherapy 2022年3月
担当区分：責任著者 掲載種別：研究論文（学術雑誌）
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Rapid inactivation of Dabie bandavirus (SFTSV) by irradiation with deep-ultraviolet light-emitting diode. 査読あり
Kaneko C, Saito A, Inagaki H, Sugiyama H, Mazimpaka E, Fujimoto S, Okabayashi T.
Journal of Medical Virology 2022年3月
担当区分：責任著者 掲載種別：研究論文（学術雑誌）
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Serological and molecular survey of tick borne pathogens, severe fever with thrombocytopenia syndrome (SFTS) virus, spotted fever group rickettsiae, and Orientia tsutsugamushi, in wild boars (Sus scrofa) in Miyazaki prefecture, Japan. 査読あり
Kirino Y, Yamamoto S, Nomachi Ngan MY, Sato Y, Sudaryatma PE, Junzo Norimine J, Fujii Y, Ando S, Okabayashi T.
Veterinary Medicine and Science 2022年3月
担当区分：責任著者 掲載種別：研究論文（学術雑誌）
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Seroprevalence of severe fever with thrombocytopenia syndrome virus in small-animal veterinarians and nurses in the Japanese prefecture with the highest case load. 査読あり
Kirino Y, Ishijima K, Miura M, Nomachi T, Mazimpaka E, Sudaryatma PE, Yamanaka A, Maeda K, Sugimoto T, Saito A, Mekata H, Okabayashi T.
Viruses 2021年2月
担当区分：責任著者 掲載種別：研究論文（学術雑誌）
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Direct TaqMan assay for the detection and genotyping of bovine viral diarrhea virus types 1 and 2
Ullah S., Notsu K., Saito A., Okabayashi T., Mekata H., Isoda N., Sekiguchi S.
Archives of Virology 170 ( 1 ) 15 2025年1月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Archives of Virology
Bovine viral diarrhea (BVD), caused by bovine viral diarrhea virus (BVDV), has a significant economic impact on affected farms worldwide. For effective disease control, it is crucial to select an appropriate vaccine based on the specific genotype of BVDV. Therefore, developing a rapid and reliable assay to detect and genotype BVDV is imperative for controlling the spread of disease. In this study, we developed a TaqMan assay to detect and genotype BVDV types 1 and 2 directly in bovine serum without extraction of RNA. The direct BVDV TaqMan assay effectively detected both BVDV1 and BVDV2 with confirmed specificity and showed no cross-reactivity with any of the other viruses tested, including bovine respiratory syncytial virus, bovine coronavirus, Akabane virus, bovine herpesvirus 1, bovine parainfluenza virus 3, bovine immunodeficiency virus, and bovine leukemia virus. The assay could detect the virus in serum samples with a titer as low as 102 TCID50/mL in two out of three trials for BVDV1 and all three trials for BVDV2, indicating that its sensitivity is equivalent to that of virus isolation. Our findings represent a significant advancement in BVDV detection and typing directly from bovine serum.
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Indonesian Herb Extracts Inhibit the Replication of Bovine Respiratory Syncytial Virus: In Vitro Study 査読あり 国際共著
Bambang Pontjo Priosoeryanto1,3 , Putu Eka Sudaryatma5,6 , Nafrina Lanniari1 , Surachmi Setiyaningsih2 , Eva Harlina1, Retno Wulansari1, Rachmi Ridho4, Riski Rostantinata1, Putu Angga Wiradana8, Tamaki Okabayashi5,6,7*
HAYATI Journal of Biosciences 32 ( 3 ) 2025年
担当区分：最終著者, 責任著者 記述言語：英語 掲載種別：研究論文（学術雑誌）
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Mekata H., Yamada K., Umeki K., Yamamoto M., Ochi A., Umekita K., Kobayashi I., Hirai T., Okabayashi T.
BMC Veterinary Research 20 ( 1 ) 190 2024年12月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：BMC Veterinary Research
Severe fever with thrombocytopenia syndrome (SFTS) is a fatal zoonosis caused by ticks in East Asia. As SFTS virus (SFTSV) is maintained between wildlife and ticks, seroepidemiological studies in wildlife are important to understand the behavior of SFTSV in the environment. Miyazaki Prefecture, Japan, is an SFTS-endemic area, and approximately 100 feral horses, called Misaki horses (Equus caballus), inhabit Cape Toi in Miyazaki Prefecture. While these animals are managed in a wild-like manner, their ages are ascertainable due to individual identification. In the present study, we conducted a seroepidemiological survey of SFTSV in Misaki horses between 2015 and 2023. This study aimed to understand SFTSV infection in horses and its transmission to wildlife. A total of 707 samples from 180 feral horses were used to determine the seroprevalence of SFTSV using enzyme-linked immunosorbent assay (ELISA). Neutralization testing was performed on 118 samples. In addition, SFTS viral RNA was detected in ticks from Cape Toi and feral horses. The overall seroprevalence between 2015 and 2023 was 78.5% (555/707). The lowest seroprevalence was 55% (44/80) in 2016 and the highest was 92% (76/83) in 2018. Seroprevalence was significantly affected by age, with 11% (8/71) in those less than one year of age and 96.7% (435/450) in those four years of age and older (p < 0.0001). The concordance between ELISA and neutralization test results was 88.9% (105/118). SFTS viral RNA was not detected in ticks (n = 516) or feral horses. This study demonstrated that horses can be infected with SFTSV and that age is a significant factor in seroprevalence in wildlife. This study provides insights into SFTSV infection not only in horses but also in wildlife in SFTS-endemic areas.
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Complete genome sequence of the avian paramyxovirus serotype 9 strain duck/Miyazaki/128/2021
Mekata H., Yamamoto M., Matsui Y., Niazi A.M., Yamada K., Okabayashi T., Cha S.Y., Jang H.K.
Microbiology Resource Announcements 13 ( 9 ) e0006024 2024年9月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Microbiology Resource Announcements
Here, we report the complete genome sequence of the avian paramyxovirus serotype 9 strain duck/Miyazaki/128/2021, which was determined using the Illumina MiSeq platform. The position of the hemagglutinin-neuraminidase stop codon differed from that of the only other available completely sequenced prototype strain, duck/New York/22/1977.
DOI： 10.1128/mra.00060-24
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Conserved Antagonization of Type I Interferon Signaling by Arterivirus GP5 Proteins
Ringo R.S., Choonnasard A., Okabayashi T., Saito A.
Viruses 16 ( 8 ) 2024年8月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Viruses
Arteriviruses can establish persistent infections in animals such as equids, pigs, nonhuman primates, rodents, and possums. Some Arteriviruses can even cause overt and severe diseases such as Equine Arteritis in horses and Porcine Reproductive and Respiratory Syndrome in pigs, leading to huge economic losses. Arteriviruses have evolved viral proteins to antagonize the host cell’s innate immune responses by inhibiting type I interferon (IFN) signaling, assisting viral evasion and persistent infection. So far, the role of the Arterivirus glycoprotein 5 (GP5) protein in IFN signaling inhibition remains unclear. Here, we investigated the inhibitory activity of 47 Arterivirus GP5 proteins derived from various hosts. We demonstrated that all GP5 proteins showed conserved activity for antagonizing TIR-domain-containing adapter proteins inducing interferon-β (TRIF)-mediated IFN-β signaling through TRIF degradation. In addition, Arterivirus GP5 proteins showed a conserved inhibitory activity against IFN-β signaling, induced by either pig or human TRIF. Furthermore, certain Arterivirus GP5 proteins could inhibit the induction of IFN-stimulated genes. These findings highlight the role of Arterivirus GP5 proteins in supporting persistent infection.
DOI： 10.3390/v16081240
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Conserved Functions of Orthohepadnavirus X Proteins to Inhibit Type-I Interferon Signaling
Choonnasard A., Shofa M., Okabayashi T., Saito A.
International Journal of Molecular Sciences 25 ( 7 ) 2024年4月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：International Journal of Molecular Sciences
Orthohepadnavirus causes chronic hepatitis in a broad range of mammals, including primates, cats, woodchucks, and bats. Hepatitis B virus (HBV) X protein inhibits type-I interferon (IFN) signaling, thereby promoting HBV escape from the human innate immune system and establishing persistent infection. However, whether X proteins of Orthohepadnavirus viruses in other species display a similar inhibitory activity remains unknown. Here, we investigated the anti-IFN activity of 17 Orthohepadnavirus X proteins derived from various hosts. We observed conserved activity of Orthohepadnavirus X proteins in inhibiting TIR-domain-containing adaptor protein inducing IFN-β (TRIF)-mediated IFN-β signaling pathway through TRIF degradation. X proteins from domestic cat hepadnavirus (DCH), a novel member of Orthohepadnavirus, inhibited mitochondrial antiviral signaling protein (MAVS)-mediated IFNβ signaling pathway comparable with HBV X. These results indicate that inhibition of IFN signaling is conserved in Orthohepadnavirus X proteins.
DOI： 10.3390/ijms25073753
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Hirohisa Mekata 1 , Ikuo Kobayashi 2 , Tamaki Okabayashi 3
Ticks and Tick-borne Diseases 14 ( 6 ) 102237 2023年11月
担当区分：最終著者 記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Ticks and Tick-borne Diseases
New technologies have led to the discovery of novel tick-borne and tick-associated viruses. Dabieshan tick virus (DaTV) and Okutama tick virus (OkTV), which belong to the family Phenuiviridae, were discovered in ticks in China and Japan, respectively, in the 2010s. Although it is unknown whether these viruses cause disease in animals or humans, all tick-associated viruses have the potential to become etiological agents of infectious diseases through gene reassortment. Therefore, it is important to elucidate the ecology of these viruses, regardless of their pathogenicity. In this study, ticks were collected year-round in Cape Toi, Miyazaki Prefecture, Japan, and an epidemiological survey of tick-associated phenuiviruses was performed. A total of 516 ticks collected from the vegetation by dragging flannel sheets were used for analysis. Pan-phenuivirus reverse transcription PCR was performed on the tick samples, and DaTV and OkTV were detected. We found that 37.0% (85/230) and 23% (16/71) of nymphal and adult Haemaphysalis longicornis were infected with DaTV, respectively, and 10% (6/62) and 13% (1/8) of nymphal and adult Haemaphysalis flava were infected with OkTV, respectively. Phylogenetic analysis indicated that the DaTV identified in this study formed a unique clade that was distinct from the strains identified in China. The survey revealed that DaTV is distributed not only in China, but also in Japan. We believe that this study contributes to our understanding of the prevalence of tick-associated viruses.
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Possible Transmission of Severe Fever with the Thrombocytopenia Syndrome Virus to an Individual Who Buried an Infected Cat 査読あり
Hirohisa Mekata 1 , Takeshi Kawaguchi 2 , Kosho Iwao 2 , Kazumi Umeki 3 , Kentaro Yamada 1 4 , Kunihiko Umekita 2 5 , Tamaki Okabayashi 1 4
Jpn J INfect Dis 2023年5月
担当区分：最終著者 掲載種別：研究論文（学術雑誌）
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Heavy metals content in fresh tuna and swordfish caught from Hindian and Pacific Oceans: Health risk assessment of dietary exposure 査読あり 国際共著
Adnorita Fandah Oktariani 1 , Putu Eka Sudaryatma 2 , Yan Ramona 3 4 , I Made Gelgel Wirasuta 5 , Ida Bagus Gede Darmayasa 4 , Putu Angga Wiradana 6 , Tamaki Okabayashi 7 8
Vet World 2023年4月
担当区分：最終著者 掲載種別：研究論文（学術雑誌）
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Oktariani A.F., Sudaryatma P.E., Ramona Y., Wirasuta I.M.G., Darmayasa I.B.G., Wiradana P.A., Okabayashi T.
Veterinary World 16 ( 4 ) 858 - 868 2023年4月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Veterinary World
Background and Aim: Yellowfin tuna and swordfish are seafood commodities commonly caught from deep oceans worldwide. Therefore, this study aimed to assess the levels of three heavy metals, namely, cadmium (Cd), lead (Pb), and mercury (Hg) in yellowfin tuna and swordfish. The results are expected to provide consumers with information on the safety of consuming or exporting these fishes caught in the Hindian and Pacific Oceans. Materials and Methods: Fresh yellowfin and swordfish were obtained from fishermen’s catches in FAO Fishing Zone 57 (Indian Ocean) and 71 (Pacific Ocean) and then collected at Benoa Harbor, Bali Province. The comparative method was to evaluate the levels of heavy metals in each fish. Furthermore, heavy metal concentrations, including Pb, Cd, and Hg, were determined using atomic absorption spectroscopy analysis. These results were then used to assess the safety of these fishes by calculating the estimated daily intake (EDI) and target hazard quotients-total target hazard quotients (THQs-TTHQs). Results: The analysis showed that none of the samples exceeded the threshold levels for the three heavy metals, which was specified by the Indonesian National Standard (SNI) and European Commission Regulation (CR) No. 1881/2006. The EDI and provisional tolerable weekly index (PTWI) obtained in this study were still in the safe range. However, the PTWI values for Pb in yellowfin tuna product from the Indian Ocean were higher (0.0038 mg/kg) compared to the recommended standard for the adult population. The THQ-TTHQ values of fish caught from these oceans were also within the acceptable range specified by the two agencies, indicating that they are safe for consumption by people with various age groups and for export purposes. Conclusion: The average levels of three heavy metals (Cd, Pb, and Hg) in muscle samples of yellowfin tuna and swordfish caught from the Pacific and Hindian Oceans were within the acceptable range as specified by the SNI and CR No. 1881/2006. Furthermore, the EDI and THQs values indicated that fishes caught from the Pacific and Hindian Oceans were safe for consumption. This research is still limited to assessing two capture fisheries commodities. Further research is needed on the assessment of heavy metal levels in other capture fisheries commodities in this capture zone.
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Kaneko C., Mekata H., Umeki K., Sudaryatma P.E., Irie T., Yamada K., Misawa N., Umekita K., Okabayashi T.
Ticks and Tick-borne Diseases 14 ( 2 ) 102115 2023年3月
担当区分：最終著者 記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Ticks and Tick-borne Diseases
Severe fever with thrombocytopenia syndrome (SFTS) is a fatal emerging tick-borne zoonotic disease caused by the SFTS virus (SFTSV). SFTSV infection in humans and companion animals is a matter of concern in endemic areas. Various wild animals are involved in the transmission cycle of SFTSV with vector ticks. Because the home range of medium-sized wild mammals commonly overlaps with humans’ living spheres, this study aimed to reveal the endemicity of SFTSV in such mammals. This study investigated the prevalence of antibodies against SFTSV and viral RNA in medium-sized wild mammals in Miyazaki Prefecture, Japan where human cases have been most frequently reported in Japan and performed a phylogenetic analysis to compare the detected SFTSV with those previously reported. Forty-three of 63 (68%) Japanese badgers (Meles anakuma) and 12 of 53 (23%) Japanese raccoon dogs (Nyctereutes procyonoides viverrinus) had antibodies against SFTSV. Japanese marten (n = 1), weasels (n = 4), and Japanese red fox (n = 1) were negative. Two of 63 (3%) badgers tested positive for SFTSV RNA, whereas the other species were negative. Phylogenetic analysis of the partial nucleotide sequence of SFTSV revealed that viral RNA detected from badgers exhibited 99.8% to 100% similarity to SFTSV, as previously reported in humans, cat, and ticks in the study area. This study demonstrated high seropositivity of antibodies in medium-sized wild mammals and suggested that SFTSV could be shared among these mammals, humans, and companion animals in endemic areas.
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Jia J., Zhao M., Ma K., Zhang H., Gui L., Sun H., Ren H., Okabayashi T., Zhao J.
Veterinary Sciences 10 ( 3 ) 2023年3月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Veterinary Sciences
OmpH is among the most important virulence factors of Pasteurella multocida, which mediates septicemia in yaks (Bos grunniens I) after infection with the bacteria. In the present study, yaks were infected with wild-type (WT) (P0910) and OmpH-deficient (ΔOmpH) P. multocida strains. The mutant strain was generated through the reverse genetic operation system of pathogens and proteomics technology. The live-cell bacterial count and clinical manifestations of P. multocida infection in Qinghai yak tissues (thymus, lung, spleen, lymph node, liver, kidney, and heart) were analyzed. The expression of differential proteins in the yak spleen under different treatments was analyzed using the marker-free method. We found that compared with the mutant strain, the titer of wild-type strains was significantly higher in tissues. Additionally, compared with other organs, the bacteria titer was significantly higher in the spleen. Compared with the WT p0910 strain, the mutant strain generated milder pathological changes in the tissues of yak. Proteomics analysis revealed that 57 of the 773 proteins expressed in P. multocida were significantly differentially expressed between the ΔOmpH and P0910 groups. Of the 57, 14 were over-expressed, whereas 43 were under-expressed. The differentially expressed proteins in the ΔompH group regulated the ABC transporter (ATP-powered translocation of many substrates across membranes) system, the two-component system, RNA degradation, RNA transcription, glycolysis/gluconeogenesis, biosynthesis of ubiquinone and other terpenoid-quinones, oxidative phosphorylation (citrate cycle) as well as fructose and mannose metabolism. The relationship among 54 significantly regulated proteins was analyzed using STRING. We found that WT P0910 and ΔOmpH of P. multocida infection activated the expression of ropE, HSPBP1, FERH, ATP10A, ABCA13, RRP7A, IL-10, IFN-γ, IL-17A, EGFR, and dnaJ. Overall, deletion of the OmpH gene weakened the virulence but maintained the immunogenicity of P. multocida in yak. The findings of this study provide a strong foundation for the pathogenesis of P. multocida and the management of related septicemia in yaks.
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Development of a direct duplex real-time PCR assay for rapid detection of domestic cat hepadnavirus
Shofa M., Ohkawa A., Okabayashi T., Kaneko Y., Saito A.
Journal of Veterinary Diagnostic Investigation 35 ( 2 ) 139 - 144 2023年3月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Journal of Veterinary Diagnostic Investigation
Domestic cat hepadnavirus (DCH) is a novel hepadnavirus, first identified in 2018. DCH is generally detected using conventional PCR assays, which include time-consuming agarose gel electrophoresis. We developed a rapid, sensitive, and specific real-time PCR (rtPCR) assay for the detection of the DCH genome. To streamline the procedure, our rtPCR assay was carried out using blood samples, without DNA extraction. A consensus primers/probe set was designed based on the nucleotide sequences of the surface/polymerase gene of all DCH strains available in GenBank. To exclude the possibility that the PCR reaction was blocked by anticoagulants, we also used a primers/probe set for amplifying the cat beta-actin gene as a reference gene. Our direct duplex rtPCR assay had high sensitivity, with a limit of detection of 10 copies/μL of blood for DCH. Our direct duplex rtPCR assay should be a useful tool for DCH detection and surveillance.
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Development of a direct duplex real-time PCR assay for rapid detection of domestic cat hepadnaviru 査読あり
Maya Shofa 1 2 , Akiho Ohkawa, Tamaki Okabayashi , Yasuyuki Kaneko , Akatsuki Saito
J.Vet. Diagn. Invest. 2023年2月
掲載種別：研究論文（学術雑誌）
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Development of a direct duplex real-time PCR assay for rapid detection of domestic cat hepadnavirus 査読あり
Maya Shofa 1 2 , Akiho Ohkawa 1 , Tamaki Okabayashi 1 2 3 , Yasuyuki Kaneko 4 , Akatsuki Saito 1 2 3
I. Vet. Diagn. Invest. 2023年2月
掲載種別：研究論文（学術雑誌）
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Mekata Hirohisa, Kawaguchi Takeshi, Iwao Kosho, Umeki Kazumi, Yamada Kentaro, Umekita Kunihiko, Okabayashi Tamaki
Japanese Journal of Medical Science and Biology advpub ( 0 ) 211 - 214 2023年1月
担当区分：責任著者 記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：国立感染症研究所
Severe fever with thrombocytopenia syndrome virus (SFTSV) is a causative agent of SFTS. Although SFTS is a fatal tick-borne zoonosis, the virus can infect humans without tick bite exposure. Recently, direct SFTSV transmission from companion pets to humans has become a significant problem. Herein, we reported a case of SFTSV transmission from a dead community cat to a woman who buried the cat in Miyazaki prefecture, Japan. The community cat died without a diagnosis of SFTS, and the woman buried it without precaution. Nine days later, the woman developed symptoms of SFTS. SFTS viral RNA and anti-SFTSV antibodies were detected in the woman. The animal carcass was exhumed, and tissue samples were collected to confirm viral infection in the cat. High copy numbers of viral RNA were detected. Sequences of the SFTSV M segment in samples from the cat and the woman showed 100% homology. The woman stated that she had touched blood that had leaked from the body when she buried it. On the other hand, the woman could have been infected when she took the cat to the animal hospital. This study highlights the risk of SFTSV infection via contact with sick or dead community cats.
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特集 重症熱性血小板減少症候群(SFTS)研究における最新知見--感染経路,病態,予防,治療 自然界におけるSFTSウイルス伝播様式
岡林 環樹
医学のあゆみ 284 ( 2 ) 129 - 132 2023年1月
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Giangaspero M., Okabayashi T.
Open Veterinary Journal 13 ( 4 ) 400 - 406 2023年
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Open Veterinary Journal
Background: In 2015, an unprecedented epidemic of microcephaly occurred in Brazil. Preliminary observations suggested the involvement of cofactors in the etiopathology of Zika virus-associated microcephaly. Bovine viral diarrhea virus (BVDV) was identified in fetal samples with microcephaly, originating in the state of Paraíba, and two virus sequences, obtained from the amniotic fluid collected from mothers with babies affected by Zika and microcephaly, have been characterized as two different species of BVDV, types 1 and 2. Aim: The involvement of BVDV as a co-factor in the etiopathogenesis of Zika virus-associated microcephaly was explored. Methods: A serological screening using an ELISA test was undertaken to detect antibodies against BVDV among patients referred to the Central Laboratory of Natal, Rio Grande do Norte, encompassing microcephalic babies and their mothers, mothers and pregnants not associated with microcephaly and general patients as a control group. Results: Two samples were positive out of 382 tested (0.52%). No specific relation with birth defects could be established. Conclusions: The study might suggest serological evidence of BVDV in humans. Further studies and the application of improved diagnostic tests adapted to humans are necessary to clarify the epidemiological extent and impact of BVDV.
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Wiradana P.A., Maharani A.Y., Sani M.D., Susilo R.J.K., Riandi M.I., Widhiantara I.G., Sudaryatma P.E., Okabayashi T., Mukti A.T.
IOP Conference Series: Earth and Environmental Science 1273 ( 1 ) 2023年
掲載種別：研究論文（学術雑誌） 出版者・発行元：IOP Conference Series: Earth and Environmental Science
The aim of the research is to monitor the dynamics of microbial number, water quality, and production performance in Pacific white shrimp (Litopenaeus vannamei) cultivation in Bulukumba, South Sulawesi Province, Indonesia, utilizing an intensive system. This research was carried out on seven Pacific white shrimp ponds over the duration of one maintenance cycle (84 days), from November 2019 to February 2020. Interestingly, the results showed that the highest total bacterial counts (TBC) were obtained in pond P3, namely in the range of 0.12-98.0×105 CFU/mL, the highest total Vibrio counts (TVC) were obtained in pond P5, namely in the range of 0.09-2.8×105 CFU/mL, luminous bacterial counts (LBC) were only found in ponds P1, P4, and P5 with the same range values, namely 0-100×103 CFU. The best performance of Pacific white shrimp production was found in pond P2, with a percentage of survival rate (SR) and productivity of 65 percent and 4074.5kg/ha, respectively, and the worst was found in pond P5, with a percentage of survival rate (SR) and productivity of 35 percent and 2583.66 kg/ha, respectively. Finally, the intense culture system used for Pacific white shrimp accumulates dynamics that may alter microbiology, water quality, and production performance.
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Inagaki H., Goto Y., Sugiyama H., Saito A., Okabayashi T., Watanabe K., Fujimoto S.
Frontiers in Public Health 10 1053729 2022年12月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Frontiers in Public Health
Reliable disinfection and sterilization technologies are needed to deal with the various infectious diseases spreading around the world. Furthermore, bacteria that are difficult to eliminate by ordinary disinfection are also a problem in the medical environment. We examined the germicidal effect of a newly developed deep-ultraviolet light-emitting diode (DUV-LED) prototype device (wavelength of 280 ± 5 nm; power of 0.9 to 1.4 mW/cm2) for floor sterilization against Escherichia coli (E. coli), Staphylococcus aureus (S. aureus), Mycobacterium gordonae (M. gordonae), and Bacillus subtilis (B. subtilis). This prototype device is equipped with highly practical DUV-LEDs with a high output efficiency and a long life, and was designed with consideration of the irradiation distance and the angle of the DUV-LEDs to provide a uniform irradiation rate. We found a statistically significant reduction of ≥90% in the infectious titers of both E. coli and S. aureus after irradiation for 2 s. Although acid-fast bacilli and spore-type bacilli are generally thought to be resistant to UV light irradiation compared to general bacteria, the acid-fast bacillus M. gordonae was inactivated after irradiation for 10 s, and spore-type cells of the bacillus B. subtilis were inactivated by ≥90% after irradiation for 30 s. We also found that the effects were cumulative when irradiation was performed at intervals. In the future, the usefulness of this device as an infection control measure will be evaluated in daily medical practice.
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Generation of a bovine cell line for gene engineering using an HIV-1-based lentiviral vector
Morizako N., Butlertanaka E.P., Tanaka Y.L., Shibata H., Okabayashi T., Mekata H., Saito A.
Scientific Reports 12 ( 1 ) 16952 2022年12月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Scientific Reports
Human immunodeficiency virus type 1 (HIV-1)-based lentiviral vectors are indispensable tools for gene engineering in mammalian cells. Conversely, lentiviral vector transduction is severely inhibited in bovine cells. Previous studies demonstrated that this inhibition is caused by the anti-lentiviral host factor tripartite motif containing 5 (TRIM5), which targets incoming HIV-1 virions by interacting with the viral capsid. In this study, we investigated several methods for overcoming the limited applicability of lentiviral vectors in bovine cells. First, we demonstrated that the SPRY domain of bovine TRIM5 is the major determinant of anti-viral activity. Second, we found that mutations that allow the capsid to evade rhesus macaque TRIM5α minimally rescued HIV-1 infectivity in bovine-derived MDBK cells. Third, we found that cyclosporine A, which relieves the inhibition of HIV-1 infection in monkey cells, significantly rescued the impaired HIV-1 infectivity in MDBK cells. Lastly, we successfully generated a bovine cell line lacking intact TRIM5 using the CRISPR/Cas9 technique. This TRIM5 knockout cell line displayed significantly higher susceptibility to an HIV-1-based lentiviral vector. In conclusion, our findings provide a promising gene engineering strategy for bovine cells, thereby contributing to innovations in agriculture and improvements in animal health.
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Virucidal activity and mechanism of action of cetylpyridinium chloride against SARS-CoV-2 査読あり
Okamoto N., Saito A., Okabayashi T., Komine A.
Journal of Oral and Maxillofacial Surgery, Medicine, and Pathology 34 ( 6 ) 800 - 804 2022年11月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Journal of Oral and Maxillofacial Surgery, Medicine, and Pathology
Objective: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the pathogen causing the coronavirus disease 2019 (COVID-19) global pandemic. Recent studies have shown the importance of the throat and salivary glands as sites of virus replication and transmission. The viral host receptor, angiotensin-converting enzyme 2 (ACE2), is broadly enriched in epithelial cells of the salivary glands and oral mucosae. Oral care products containing cetylpyridinium chloride (CPC) as a bactericidal ingredient are known to exhibit antiviral activity against SARS-CoV-2 in vitro. However, the exact mechanism of action remains unknown. Methods: This study examined the antiviral activity of CPC against SARS-CoV-2 and its inhibitory effect on the interaction between the viral spike (S) protein and ACE2 using an enzyme-linked immunosorbent assay. Results: CPC (0.05%, 0.1% and 0.3%) effectively inactivated SARS-CoV-2 within the contact times (20 and 60 s) in directions for use of oral care products in vitro. The binding ability of both the S protein and ACE2 were reduced by CPC. Conclusions: Our results suggest that CPC inhibits the interaction between S protein and ACE2, and thus, reduces infectivity of SARS-CoV-2 and suppresses viral adsorption.
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Survey of hepatitis E virus in pork products and pig stools in Nakhon Pathom Province, Thailand
Thippornchai N., Leaungwutiwong P., Kosoltanapiwat N., Vuong C., Nguyen K., Okabayashi T., Lee A.
Veterinary Medicine and Science 8 ( 5 ) 1975 - 1981 2022年9月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Veterinary Medicine and Science
Background: Hepatitis E virus (HEV) is an important public health threat resulting in more than 3 million symptomatic cases and 70,000 deaths annually. HEV is classified into at least eight genotypes, and five are associated with human infection. Genotypes 1 and 2 primarily affect humans, whereas genotypes 3 and 4 circulate in both humans and swine and are considered zoonotic viruses. Previous studies in Central Thailand have reported human HEV isolates with high similarity to swine strains and high seroprevalence in pigs, suggesting the potential for pig-to-human transmission. Objectives: This study aimed to detect and analyse HEV in pork products and pig stools collected from local markets and pig farms in Nakhon Pathom Province in Central Thailand. Methods: A total of 177 pig stool and 214 pork product samples were detected for HEV by using RT–PCR amplification. Next, nucleotide sequencing and phylogenetic analysis were performed. Results: We found one sample of pork products (1/214, 0.5%), which was a pig liver sample (1/51, 2.0%), and 49 HEV-positive samples in pig stools (49/177, 27.7%). Phylogenetic analysis showed that all these HEV sequences belonged to genotype 3, with a high correlation between our samples and HEV from humans and swine was previously reported in Thailand. Conclusions: This study suggested that the consumption of poorly sanitized or uncooked animal meat or food and frequent exposure to pig stools may be risk factors for HEV infections in humans.
DOI： 10.1002/vms3.854
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Sugamoto K., Tanaka Y.L., Saito A., Goto Y., Nakayama T., Okabayashi T., Kunitake H., Morishita K.
Biochemical and Biophysical Research Communications 615 ( 615 ) 56 - 62 2022年7月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Biochemical and Biophysical Research Communications
With the current worldwide pandemic of COVID-19, there is an urgent need to develop effective treatment and prevention methods against SARS-CoV-2 infection. We have previously reported that the proanthocyanidin (PAC) fraction in blueberry (BB) leaves has strong antiviral activity against hepatitis C virus (HCV) and human T-lymphocytic leukemia virus type 1 (HTLV-1). In this study, we used Kunisato 35 Gou (K35) derived from the rabbit eye blueberry (Vaccinium virgatum Aiton), which has a high PAC content in the leaves and stems. The mean of polymerization (mDP) of PAC in K35 was the highest of 7.88 in Fraction 8 (Fr8) from the stems and 12.28 of Fraction 7 (Fr7) in the leaves. The composition of BB-PAC in K35 is that most are B-type bonds with a small number of A-type bonds and cinchonain I as extension units. A strong antiviral effect was observed in Fr7, with a high polymerized PAC content in both the leaves and stems. Furthermore, when we examined the difference in the action of BB-PAC before and after SARS-CoV-2 infection, we found a stronger inhibitory effect in the pre-infection period. Moreover, BB-PAC Fr7 inhibited the activity of angiotensin II converting enzyme (ACE2), although no effect was observed in a neutralization test of pseudotyped SARS-CoV-2. The viral chymotrypsin-like cysteine protease (3CLpro) of SARS-CoV-2 was also inhibited by BB-PAC Fr7 in leaves and stems. These results indicate that BB-PAC has at least two different inhibitory effects, and that it is effective in suppressing SARS-CoV-2 infection regardless of the time of infection.
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Kaneko C., Saito A., Inagaki H., Sugiyama H., Mazimpaka E., Fujimoto S., Okabayashi T.
Journal of Medical Virology 94 ( 7 ) 3438 - 3441 2022年7月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Journal of Medical Virology
Severe fever with thrombocytopenia syndrome (SFTS) caused by Dabie bandavirus (SFTSV) is a serious public health concern in endemic areas, particularly in Asian and Southeast Asian countries. SFTSV is transmitted by direct contact with body fluids from infected humans and animals. Therefore, environmental hygiene in hospitals and veterinary clinics in SFTSV-endemic areas is highly important. This study assessed the effects of continuous and intermittent irradiation with deep-ultraviolet light-emitting diode (DUV-LED) on SFTSV. Evaluation was performed by conducting plaque assay in which SFTSV irradiated with deep-ultraviolet (DUV; 280 ± 5 nm) was inoculated onto Vero cells. The results showed that continuous and intermittent irradiation for 5 s, resulting in 18.75 mJ/cm2 of cumulative UV exposure, led to a >2.7 and >2.9 log reduction, respectively, corresponding to a >99.8% reduction in infectivity. These results demonstrate that DUV can be utilized for inactivation of SFTSV to maintain environmental hygiene in hospitals and veterinary clinics in endemic countries.
DOI： 10.1002/jmv.27698
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Global Prevalence of Domestic Cat Hepadnavirus: An Emerging Threat to Cats’ Health? 査読あり
Shofa M., Kaneko Y., Takahashi K., Okabayashi T., Saito A.
Frontiers in Microbiology 13 938154 2022年6月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Frontiers in Microbiology
Hepatitis B is an infectious hepatocellular disease of global concern caused by hepatitis B virus (HBV), which belongs to Hepadnaviridae. Recently, a novel HBV-like virus, domestic cat hepadnavirus (DCH), was detected from an immunocompromised cat with a hepatic disease in Australia. Subsequent molecular investigation by independent research groups revealed that its prevalence rates were 6.5% in Australia, 10.8% in Italy, 12.4% in Thailand, 12.3% in Malaysia, 3.08% in the United Kingdom, and 0.78% in Japan. Although the correlation between DCH infection and hepatic diseases remains to be elucidated, understanding the diversity of circulating DCH will contribute to its prevention and control in domestic cats. Herein, we summarize the current epidemiological data of DCH in these countries.
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Kirino Y., Yamanaka A., Ishijima K., Tatemoto K., Maeda K., Okabayashi T.
Journal of Infection and Chemotherapy 28 ( 6 ) 753 - 756 2022年6月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Journal of Infection and Chemotherapy
Introduction: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging tick-born disease and its animal-to-human transmission has come to attention recently. During our sero-survey of SFTS virus (SFTSV) among veterinary professionals in 2018, a veterinarian and his assistant working in an animal hospital were tested positive by enzyme-linked immunosorbent assay (ELISA). An additional survey implied a cluster of SFTS cases in which four more people, a family who brought two sick dogs to the animal hospital in 2003, were involved. This study aimed at assessing the possibility of animal-to-human transmission of SFTSV in this cluster. Methods: Retrospective interviews were performed with the owner family of the dogs and their clinical records were obtained from each hospital. SFTSV-IgG were tested by ELISA and virus neutralization test using the sera collected from them in 2018. Results: The interviews revealed that a total of six people, the two veterinary professionals and the owner family who took care of the sick dogs, suffered from SFTS-like symptoms in the same period of time in 2003. All patients did not have tick bite before the onset and all suspected causative agents were excluded by laboratory tests. The serological tests in this study revealed the four owner family members were all positive for SFTSV antibodies. Conclusions: Considering the extremely low seroprevalence of SFTSV antibodies among inhabitants of the region, the existence of SFTSV antibodies in all these six people presents a possibility that they were involved in an SFTS outbreak originated in the sick dogs in 2003.
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Identification of domestic cat hepadnavirus from a cat blood sample in Japan 査読あり
Takahashi K., Kaneko Y., Shibanai A., Yamamoto S., Katagiri A., Osuga T., Inoue Y., Kuroda K., Tanabe M., Okabayashi T., Naganobu K., Minobe I., Saito A.
Journal of Veterinary Medical Science 84 ( 5 ) 648 - 652 2022年5月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Journal of Veterinary Medical Science
The hepatitis B virus (Hepadnaviridae) induces chronic hepatitis and hepatic cancer in humans. A novel domestic cat hepadnavirus (DCH) was recently identified in several countries, however, the DCH infection status of cats in Japan is unknown. Therefore, we investigated the DCH infection rate of 139 cat samples collected in Japan. We identified one positive blood sample (0.78%) from a 17-year-old female cat with chronically elevated alanine aminotransferase. Phylogenetic analysis demonstrated that the DCH strain identified in this study is genetically different from strains in other countries. Further investigations are required to elucidate the evolution of DCH and the impact of DCH infection on hepatic diseases in domestic cats.
DOI： 10.1292/jvms.22-0010
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Whole-Genome Sequencing of Pasteurella multocida Strain Pm1, Isolated from a Calf
Gondaira S., Fujiki J., Hirano Y., Murata R., Uchida I., Usui M., Iwasaki T., Okabayashi T., Iwano H., Higuchi H.
Microbiology Resource Announcements 11 ( 4 ) e0004222 2022年4月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Microbiology Resource Announcements
Bovine pneumonia is a disease that causes significant economic losses in livestock industries and is vital for animal welfare. The whole-genome sequence of Pasteurella multocida strain Pm1, isolated from a calf suffering from pneumonia in Japan, is reported here.
DOI： 10.1128/mra.00042-22
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Kotaki T., Kurosu T., Grinyo-Escuer A., Davidson E., Churrotin S., Okabayashi T., Puiprom O., Mulyatno K.C., Sucipto T.H., Doranz B.J., Ono K.i., Soegijanto S., Kameoka M.
Scientific Reports 11 ( 1 ) 12987 2021年12月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Scientific Reports
Dengue virus (DENV), from the genus flavivirus of the family flaviviridae, causes serious health problems globally. Human monoclonal antibodies (HuMAb) can be used to elucidate the mechanisms of neutralization and antibody-dependent enhancement (ADE) of DENV infections, leading to the development of a vaccine or therapeutic antibodies. Here, we generated eight HuMAb clones from an Indonesian patient infected with DENV. These HuMAbs exhibited the typical characteristics of weak neutralizing antibodies including high cross-reactivity with other flaviviruses and targeting of the fusion loop epitope (FLE). However, one of the HuMAbs, 3G9, exhibited strong neutralization (NT50 < 0.1 μg/ml) and possessed a high somatic hyper-mutation rate of the variable region, indicating affinity-maturation. Administration of this antibody significantly prolonged the survival of interferon-α/β/γ receptor knockout C57BL/6 mice after a lethal DENV challenge. Additionally, Fc-modified 3G9 that had lost their in vitro ADE activity showed enhanced therapeutic potency in vivo and competed strongly with an ADE-prone antibody in vitro. Taken together, the affinity-matured FLE-targeting antibody 3G9 exhibits promising features for therapeutic application including a low NT50 value, potential for treatment of various kinds of mosquito-borne flavivirus infection, and suppression of ADE. This study demonstrates the therapeutic potency of affinity-matured FLE-targeting antibodies.
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Izzati U.Z., Kaneko Y., Kaneko C., Yoshida A., Suwanruengsri M., Okabayashi T., Hirai T., Yamaguchi R.
Journal of Comparative Pathology 188 44 - 51 2021年10月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Journal of Comparative Pathology
Following isolation of pseudorabies virus (PRV) from two hunting dogs in Oita prefecture, Japan, we investigated the PRV antigen distribution in the tissues of the infected animals. At necropsy, PRV-associated lesions included facial oedema, tonsillar and meningeal congestion, blotchy haemorrhages on the pericardium and mitral valves, and incomplete splenic contraction in one dog, with less prominent findings in the other dog. Multiple pulmonary nodules were seen in both cases, caused by the diploid form of Paragonimus westermani lung flukes, as confirmed by PCR-restriction fragment length polymorphism and gene sequencing analyses. Histological examination revealed that the PRV infection was associated with lesions of non-suppurative encephalitis in the brainstem. PRV antigen was detected in the cerebrum, cerebellum, brainstem, submucosal and myenteric plexuses, and mononuclear cells, mainly in the bone marrow, lymph nodes, tonsils and spleen tissues. There was evidence of PRV dissemination to the brain via the trigeminal or olfactory routes, in addition to possible spread to lymphoid organs via infected mononuclear cells.
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Oba M., Rongduo W., Saito A., Okabayashi T., Yokota T., Yasuoka J., Sato Y., Nishifuji K., Wake H., Nibu Y., Mizutani T.
Biochemical and Biophysical Research Communications 570 21 - 25 2021年9月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Biochemical and Biophysical Research Communications
Natto, a traditional Japanese fermented soybean food, is well known to be nutritious and beneficial for health. In this study, we examined whether natto impairs infection by viruses, such as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) as well as bovine herpesvirus 1 (BHV-1). Interestingly, our results show that both SARS-CoV-2 and BHV-1 treated with a natto extract were fully inhibited infection to the cells. We also found that the glycoprotein D of BHV-1 was shown to be degraded by Western blot analysis and that a recombinant SARS-CoV-2 receptor-binding domain (RBD) was proteolytically degraded when incubated with the natto extract. In addition, RBD protein carrying a point mutation (UK variant N501Y) was also degraded by the natto extract. When the natto extract was heated at 100 °C for 10 min, the ability of both SARS-CoV-2 and BHV-1 to infect to the cells was restored. Consistent with the results of the heat inactivation, a serine protease inhibitor inhibited anti-BHV-1 activity caused by the natto extract. Thus, our findings provide the first evidence that the natto extract contains a protease(s) that inhibits viral infection through the proteolysis of the viral proteins.
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Srijangwad A., Tripipat T., Saeng-chuto K., Jermsujarit P., Tantituvanont A., Okabayashi T., Nilubol D.
Journal of Immunological Methods 494 113045 2021年7月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Journal of Immunological Methods
The objectives of this study are to develop and optimize indirect ELISA based on three coating antigens of porcine epidemic diarrhea virus (PEDV), recombinant spike (S12), nucleocapsid (N), and whole viral (WV) proteins, for the detection of IgG and IgA antibodies in colostrum and milk and to evaluate the diagnostic sensitivity (DSe) and diagnostic specificity (DSp) of the assay as a diagnostic method. Colostrum (n = 347) and milk (n = 272) samples from sows were employed in this assay. Indirect ELISA based on three coating antigens was assessed by receiver operating characteristic (ROC) curve analysis with a virus neutralization (VN) test as a reference method, and the cutoff value for calculating DSe and DSp was determined. S12-ELISA showed higher DSe and DSp of IgG and IgA detection compared to N- and WV-ELISA in both colostrum and milk samples. Moreover, S12-ELISA showed perfect agreement and a high correlation with the VN test, which was better than the N- and WV-ELISA for both IgG and IgA detection in colostrum and milk. In contrast, N-ELISA showed lower DSe and DSp compared to S12- and WV-ELISA, along with a correlation with VN and substantial agreement with the VN test. Nevertheless, our developed ELISAs have accuracy for repeatability in both inter- and intra-assay variation. Overall, this research demonstrates that S12-ELISA is more suitable than WV- and N-ELISA to detect IgG and IgA antibodies against PEDV from both colostrum and milk samples.
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Inagaki H., Saito A., Kaneko C., Sugiyama H., Okabayashi T., Fujimoto S.
Pathogens 10 ( 6 ) 2021年6月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Pathogens
More than 1 year has passed since social activities have been restricted due to the spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). More recently, novel SARS-CoV-2 variants have been spreading around the world, and there is growing concern that they may have higher transmissibility and that the protective efficacy of vaccines may be weaker against them. Immediate measures are needed to reduce human exposure to the virus. In this study, the antiviral efficacy of deep-ultraviolet light-emitting diode (DUV-LED) irradiation (280 ± 5 nm, 3.75 mW/cm2 ) against three SARS-CoV-2 variants was evaluated. For the B.1.1.7, B.1.351, and P.1 variant strains, irradiation of the virus stocks for 1 s resulted in infectious titer reduction rates of 96.3%, 94.6%, and 91.9%, respectively, and with irradiation for 5 s, the rates increased to 99.9%, 99.9%, and 99.8%, respectively. We also tested the effect of pulsed DUV-LED irradiation (7.5 mW/cm2, duty rate: 50%, frequency: 1 kHz) under the same output conditions as for continuous irradiation and found that the antiviral efficacy of pulsed and continuous irradiation was the same. These findings suggest that by further developing and optimizing the DUV-LED device to increase its output, it may be possible to instantly inactivate SARS-CoV-2 with DUV-LED irradiation.
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Fahkrajang W., Sudaryatma P.E., Mekata H., Hamabe S., Saito A., Okabayashi T.
Veterinary Microbiology 255 109017 2021年4月
担当区分：責任著者 記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Veterinary Microbiology
Bovine coronavirus (BCoV) is one of the agents causing bovine respiratory disease complex (BRDC), with single infection tending to be mild to moderate; the probability of developing pneumonia in BRDC may be affected by viral and bacterial combinations. Previously, we reported that bovine respiratory syncytial virus (BRSV) infection enhances adherence of Pasteurella multocida (PM) to cells derived from the bovine lower respiratory tract but that BRSV infection in cells derived from the upper respiratory tract reduces PM adherence. In this study, we sought to clarify whether the modulation of bacterial adherence to cells derived from the bovine upper and lower respiratory tract is shared by other BRDC-related viruses by infecting bovine epithelial cells from the trachea, bronchus and lung with BCoV and/or PM. The results showed that cells derived from both the upper and lower respiratory tract were susceptible to BCoV infection. Furthermore, all cells infected with BCoV exhibited increased PM adherence via upregulation of two major bacterial adhesion molecules, intercellular adhesion molecule-1 (ICAM-1) and platelet-activating factor receptor (PAF-R), suggesting that compared with BRSV infection, BCoV infection differentially modulates bacterial adherence. In summary, we identified distinct interaction between bovine respiratory viruses and bacterial infections.
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Nagai M, Okabayashi T, Akagami M, Matsuu A, Fujimoto Y, Hashem MA, Mekata H, Nakao R, Matsuno K, Katayama Y, Oba M, Omatsu T, Asai T, Nakagawa K, Ito H, Madarame H, Kawai K, Ito T, Nonaka N, Tsukiyama-Kohara K, Inoshima Y, Mizutani T, Misawa N
Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases 88 104664 2021年3月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Infection, Genetics and Evolution
Recently, hepe-astrovirus-like RNA viruses named bastroviruses (BastVs), have been found in human, pig, bat, and rat fecal samples. In this study, we determined nearly complete genome sequences of four BastVs in the feces of healthy pigs. Genetic characterization revealed that these porcine BastVs (PBastVs) and BastVs from other animals including humans, had the same genome organization, that is, they contained three predicted conserved domains of viral methyltransferase, RNA helicase, and RdRp in the nonstructural ORF1 and the astrovirus capsid domain in the structural ORF2. Phylogenetic analyses using RNA-dependent RNA polymerase and the capsid region revealed that PBastVs branched with bat and rat BastVs; however, the groups formed by each host were distantly related to human BastVs. Pairwise amino acid sequence comparison demonstrated that PBastVs shared 95.2–98.6% and 76.1–95.5% sequence identity among each other in the ORF1 and ORF2 regions, respectively; the sequence identities between PBastVs and BastVs from other animals were 21.4–42.5% and 9.1–20.6% in the ORF1 and ORF2 regions, respectively. This suggested that BastVs were derived from a common ancestor but evolved independently in each host population during a prolonged period. Putative recombination events were identified in the PBastV genome, suggesting that PBastVs gain sequence diversity and flexibility through recombination events. In an analysis of previously obtained metagenomic data, PBastV sequence reads were detected in 7.3% (23/315) of fecal samples from pigs indicating that PBastVs are distributed among pig populations in Japan.
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Kabali E., Pandey G.S., Munyeme M., Kapila P., Mukubesa A.N., Ndebe J., Muma J.B., Mubita C., Muleya W., Muonga E.M., Mitoma S., Hang’ombe B.M., Wiratsudakul A., Ngan M.T., Elhanafy E., El Daous H., Huyen N.T., Yamazaki W., Okabayashi T., Abe M., Norimine J., Sekiguchi S.
Antibiotics 10 ( 3 ) 1 - 17 2021年3月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Antibiotics
A cross-sectional study was used to identify and assess prevalence and phenotypic antimicrobial resistance (AMR) profiles of Escherichia coli and other enterobacteria isolated from healthy wildlife and livestock cohabiting at a 10,000 acres game ranch near Lusaka, Zambia. Purposive sampling was used to select wildlife and livestock based on similarities in behavior, grazing habits and close interactions with humans. Isolates (n = 66) from fecal samples collected between April and August 2018 (n = 84) were examined following modified protocols for bacteria isolation, biochemical identification, molecular detection, phylogenetic analysis, and antimicrobial susceptibility testing by disc diffusion method. Data were analyzed using R software, Genetyx ver.12 and Mega 6. Using Applied Profile Index 20E kit for biochemical identification, polymerase chain reaction assay and sequencing, sixty-six isolates were identified to species level, of which Escherichia coli (72.7%, 48/66), E. fergusonii (1.5%, 1/66), Shigella sonnei (22.7%, 14/66), Sh. flexinerri (1.5%, 1/66) and Enterobacteriaceae bacterium (1.5%, 1/66), and their relationships were illustrated in a phylogenetic tree. Phenotypic antimicrobial resistance or intermediate sensitivity expression to at least one antimicrobial agent was detected in 89.6% of the E. coli, and 73.3% of the Shigella isolates. The E. coli isolates exhibited the highest resistance rates to ampicillin (27%), ceftazidime (14.3%), cefotaxime (9.5%), and kanamycin (9.5%). Multidrug resistance (MDR) was detected in 18.8% of E. coli isolates while only 13.3% Shigella isolates showed MDR. The MDR was detected among isolates from impala and ostrich (wild animals in which no antimicrobial treatment was used), and in isolates from cattle, pigs, and goats (domesticated animals). This study indicates the possible transmission of drug-resistant microorganisms between animals cohabiting at the wildlife-livestock interface. It emphasizes the need for further investigation of the role of wildlife in the development and transmission of AMR, which is an issue of global concern.
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Sato Y, Mekata H, Sudaryatma PE, Kirino Y, Yamamoto S, Ando S, Sugimoto T, Okabayashi T
Vector borne and zoonotic diseases (Larchmont, N.Y.) 21 ( 5 ) 378 - 384 2021年2月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Vector-Borne and Zoonotic Diseases
Severe fever with thrombocytopenia syndrome (SFTS), caused by Dabie bandavirus, generally called SFTS virus (SFTSV), is an emerging zoonosis in East Asia. In Japan, 50-100 cases of SFTS have been reported each year since the first case was reported in 2013. SFTS is a tick-borne infectious disease, and SFTSV has been isolated from ticks in China and South Korea. Haemaphysalis longicornis and Amblyomma testudinarium are considered the primary vectors in Japan. However, the other tick species seldom feeding on humans might also play an important role in maintaining the virus in nature. In this study, we collected ticks on vegetation around the location where two SFTS patients were estimated to have been infected in Miyazaki Prefecture, Japan, isolated live SFTSV, and performed a phylogenetic analysis. A total of 257 ticks were collected, and SFTSV RNA was detected in 19.5% (9/46) of tick pools. A total of 10 infectious SFTSVs were successfully isolated from A. testudinarium, Haemaphysalis flava, Haemaphysalis formosensis, Haemaphysalis hystricis, and Haemaphysalis megaspinosa. Furthermore, the whole viral sequences isolated from ticks were highly homologous to sequences isolated from SFTS patients in the same sampling area in the past. These results suggest that SFTSVs are maintained in these tick species in the sampling area and sporadically transmitted to humans. Surveillance of SFTSV in ticks provides important information about the risk of incidental transmission to humans.
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Kirino Y, Ishijima K, Miura M, Nomachi T, Mazimpaka E, Sudaryatma PE, Yamanaka A, Maeda K, Sugimoto T, Saito A, Mekata H, Okabayashi T
Viruses 13 ( 2 ) 2021年2月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Viruses
Severe fever with thrombocytopenia syndrome virus (SFTSV) is the causative agent of SFTS, an emerging tick-borne disease in East Asia, and is maintained in enzootic cycles involving ticks and a range of wild animal hosts. Direct transmission of SFTSV from cats and dogs to humans has been identified in Japan, suggesting that veterinarians and veterinary nurses involved in smallanimal practice are at occupational risk of SFTSV infection. To characterize this risk, we performed a sero-epidemiological survey in small-animal-practice workers and healthy blood donors in Miyazaki prefecture, which is the prefecture with the highest per capita number of recorded cases of SFTS in Japan. Three small-animal-practice workers were identified as seropositive by ELISA, but one had a negative neutralization-test result and so was finally determined to be seronegative, giving a seropositive rate of 2.2% (2 of 90), which was significantly higher than that in healthy blood donors (0%, 0 of 1000; p < 0.05). The seroprevalence identified here in small-animal-practice workers was slightly higher than that previously reported in other high-risk workers engaged in agriculture and forestry in Japan. Thus, enhancement of small-animal-practice workers’ awareness of biosafety at animal hospitals is necessary for control of SFTSV.
DOI： 10.3390/v13020229
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Ito S, Hirai T, Hamabe S, Subangkit M, Okabayashi T, Goto Y, Nishida S, Kurita T, Yamaguchi R
The Journal of veterinary medical science 83 ( 1 ) 146 - 150 2021年1月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：公益社団法人 日本獣医学会
On a coastline in Miyazaki Prefecture, Japan, a wild subadult female striped dolphin was found dead. Necropsy revealed poor nutritional status and bilateral pneumonia, which was histologically diagnosed as severe suppurative necrotizing bronchopneumonia. Special staining detected numerous intralesional filamentous, branching bacteria, which was identified as <i>Nocardia cyriacigeorgica</i> by sequencing of 16S ribosomal RNA and gyrB genes. Other main histological findings included lymphoid depletion in the spleen and superficial cervical and pulmonary lymph nodes. Suppurative nocardiosis without a granulomatous reaction is uncommon, and it is assumed its pathogenesis was related to the host's immune status. This paper discusses the variable inflammatory response to nocardiosis and describes the first case of <i>N. cyriacigeorgica</i> infection in a wild striped dolphin in Japan.
DOI： 10.1292/jvms.20-0234
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Rapid Inactivation of SARS-CoV-2 with Ozonated Water
Inagaki H., Saito A., Sudaryatma P.E., Sugiyama H., Okabayashi T., Fujimoto S.
Ozone: Science and Engineering 43 ( 3 ) 208 - 212 2021年
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Ozone: Science and Engineering
Although ozonated water is one of the promising candidates for hand hygiene to prevent fomite infection, the detailed effects of ozonated water on SARS-CoV-2 have not been clarified. We evaluated the inactivating effect of ozonated water against SARS-CoV-2 by its concentration and exposure time. The reduction rates of virus titer after 5 sec treatment with ozone concentrations of 1, 4, 7, and 10 mg/L were 81.4%, 93.2%, 96.6%, and 96.6%, respectively. No further decrease in virus titer was observed by the extended exposure time over 5 sec. High-concentration ozonated water, such as more than 7 mg/L, was considered to be effective in promptly inactivating SARS-CoV-2 virus in aqueous solution.
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Kirino Y., Yamamoto S., Nomachi T., Mai T.N., Sato Y., Sudaryatma P.E., Norimine J., Fujii Y., Ando S., Okabayashi T.
Veterinary Medicine and Science 8 ( 2 ) 877 - 885 2021年
担当区分：責任著者 記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Veterinary Medicine and Science
Background: Miyazaki Prefecture is one of the hotspots of severe fever with thrombocytopenia syndrome (SFTS) cases and related deaths in Japan since 2013 and other pathogens of tick-borne diseases (TBDs). Japanese spotted fever and scrub typhus are also endemic in this region. Objectives: A total of 105 wild boars, hunted in 2009, were serologically examined as sentinels for TBDs to indirectly demonstrate the potential hazard of ticks transmitting pathogens to humans in the studied area. Methods: The collected blood and spleens of the wild boars underwent serological and molecular tests for SFTSV, Rickettsia japonica (Rj) [antibody to spotted fever group rickettsiae (SFGR) were tested by using species-common antigen], and Orientia tsutsugamushi (Ot). Results: Seroprevalences of SFTSV, SFGR, and Ot were 41.9%, 29.5%, and 33.3%, respectively. SFTS viral RNA was identified in 7.6% of the sera, whereas DNA of Rj or Ot was not detected in any sample. In total, 43.8% of the boars possessed an infection history with SFTSV (viral gene and/or antibody). Of these, 23.8% had multiple-infection history with SFGR and/or Ot. Conclusions: The high prevalence of SFTSV in wild boars might reflect the high risk of exposure to the virus in the studied areas. In addition, SFTSV infection was significantly correlated with Ot infection, and so were SFGR infection and Ot infection, indicating that these pathogens have common factors for infection or transmission. These data caution of the higher risk of SFTSV infection in areas with reported cases of other TBDs.
DOI： 10.1002/vms3.696
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岡林 環樹
産学官連携ジャーナル 17 ( 9 ) 25 - 28 2021年
担当区分：筆頭著者 記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：国立研究開発法人 科学技術振興機構
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Rapid inactivation of SARS-CoV-2 with deep-UV LED irradiation. 査読あり
Saito A2,
Emerging Microbes and Infections 2020年12月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Sekiguchi Y., Nagata A., Sunaga F., Oi T., Imai R., Madarame H., Katayama Y., Oba M., Okabayashi T., Misawa N., Oka T., Mizutani T., Nagai M.
Archives of Virology 165 ( 12 ) 2909 - 2914 2020年12月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Archives of Virology
© 2020, Springer-Verlag GmbH Austria, part of Springer Nature. Two and three genotypes of enterovirus G (EV-G) carrying a papain-like cysteine protease (PL-CP) sequence were detected on two pig farms and classified into genotypes G1 and G10, and G1, G8, and G17, respectively, based on VP1 sequences. A G10 EV-G virus bearing a PL-CP sequence was detected for the first time. Phylogenetic analysis of the P2 and P3 regions grouped the viruses by farm with high sequence similarity. Furthermore, clear recombination break points were detected in the 2A region, suggesting that PL-CP EV-G-containing strains gained sequence diversity through recombination events among the multiple circulating EV-G genotypes on the farms.
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Yamanaka A., Kirino Y., Fujimoto S., Ueda N., Himeji D., Miura M., Sudaryatma P.E., Sato Y., Tanaka H., Mekata H., Okabayashi T.
Emerging Infectious Diseases 26 ( 12 ) 2994 - 2998 2020年12月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Emerging Infectious Diseases
© 2020 Centers for Disease Control and Prevention (CDC). All rights reserved. Two veterinary personnel in Japan were infected with severe fever with thrombocytopenia syndrome virus (SFTSV) while handling a sick cat. Whole-genome sequences of SFTSV isolated from the personnel and the cat were 100% identical. These results identified a nosocomial outbreak of SFTSV infection in an animal hospital without a tick as a vector.
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Seroprevalence of Flavivirus Neutralizing Antibodies in Thailand by High-Throughput Neutralization Assay: Endemic Circulation of Zika Virus before 2012 査読あり
Atsushi Yamanaka, Mami Matsuda , Tamaki Okabayashi, Pannamthip Pitaksajjakul , Pongrama Ramasoota , Kyoko Saito, Masayoshi Fukasawa, Kentaro Hanada, Tomokazu Matsuura, Masamichi Muramatsu, Tatsuo Shioda, Ryosuke Suzuki
mSphere 6 ( 4 ) 2020年8月
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Heavy Metals in Tuna Species Meat and Potential Consumer Health Risk: A Review
Norhazirah A.A., Shazili N.A.M., Kamaruzzaman Y., Sim S.F., Ahmad A., Ong M.C.
IOP Conference Series: Earth and Environmental Science 494 ( 1 ) 2020年8月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：IOP Conference Series: Earth and Environmental Science
Marine fishes are one of the protein sources and they form a significant part of human diet around the world. In particular, tuna fisheries are considered as the largest and most specialized commercially important group of species among all commercial fishes. The preparing tuna methods such as cooking and canning might alter the level of heavy metals inside the meat. The heat that applied to the meat might be decreased the moisture content in tuna meat, thus give some effects to the heavy metal level. Other than that, the different composition and function of different parts of tuna can contribute to the various level of heavy metals analysed. The metal contamination in tuna species meat has put serious question to the safety level of fish intake to the community. It represent an abiding threat to human health as it has been linked to some adverse health effects such as mental retardation, kidney damage, and various types of cancer and even worse, death could occur.The following review articles presents the findings of the work carried out by the various researchers in the past on the heavy metal pollution in samples of Tuna species around the world.
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Bovine respiratory syncytial virus enhances the adherence of Pasteurella multocida to bovine respiratory tract epithelial cells by upregulating the Platelet-acting factor receptor. 査読あり
Sudaryatma P., Saito A., Mekara H., Kubo M., Fahkrajang W., Mazimpaka E., Okabayashi T.
Frontiers in Microbiology 2020年7月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Molecular epidemiological survey and phylogenetic analysis of bovine respiratory coronavirus in Japan from 2016 to 2018 査読あり
Hirohisa MEKATA, Saori HAMABE, Putu Eka SUDARYATMA, Ikuo KOBAYASHI, Toru KANNO, Tamaki OKABAYASHI
2020年6月
記述言語：英語 掲載種別：研究論文（学術雑誌）
DOI： 10.1292/jvms.19-0587
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Bovine respiratpry Syncytiak Virus decreased Pasteurella multocida adherence by downregulation the expression of intercellurar adhesion molecule-1 on the surface of upper respiratory epitheliacl cells. 査読あり
Sudaryatma PE,Saito A,Mekata H,Kubo M,Fahkrajang W,Tamaki Okabayashi T
Veterinary Microbiology 2020年6月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Nagata A., Sekiguchi Y., Oi T., Sunaga F., Madarame H., Imai R., Sano K., Katayama Y., Omatsu T., Oba M., Furuya T., Shirai J., Okabayashi T., Misawa N., Oka T., Mizutani T., Nagai M.
Journal of General Virology 101 ( 8 ) 840 - 852 2020年
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Journal of General Virology
© 2020 Microbiology Society. All rights reserved. The genetic diversity of enterovirus G (EV-G) was investigated in the wild-boar population in Japan. EV-G-specific reverse transcription PCR demonstrated 30 (37.5%) positives out of 80 faecal samples. Of these, viral protein 1 (VP1) fragments of 20 samples were classified into G1 (3 samples), G4 (1 sample), G6 (2 samples), G8 (4 samples), G11 (1 sample), G12 (7 samples), G14 (1 sample) and G17 (1 sample), among which 11 samples had a papain-like cysteine protease (PL-CP) sequence, believed to be the first discoveries in G1 (2 samples) or G17 (1 sample) wild-boar EV-Gs, and in G8 (2 samples) or G12 (6 samples) EV-Gs from any animals. Sequences of the non-structural protein regions were similar among EV-Gs possessing the PL-CP sequence (PL-CP EV-Gs) regardless of genotype or origin, suggesting the existence of a common ancestor for these strains. Interestingly, for the two G8 and two G12 samples, the genome sequences contained two versions, with or without the PL-CP sequence, together with the homologous 2C/PL-CP and PL-CP/3A junction sequences, which may explain how the recombination and deletion of the PL-CP sequences occured in the PL-CP EV-G genomes. These findings shed light on the genetic plasticity and evolution of EV-G.
DOI： 10.1099/jgv.0.001446
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Slaughterhouse survey for detection of bovine viral diarrhea infection among beef cattle in Kyushu, Japan. 査読あり
Agah MA, Notsu K, El-Khaiat HM, Arikawa G, Kubo M, Mitoma S, Okabayashi T, Mekata H, Elhanafy E, El Daous H, Mai TN, Nguyen TH, Isoda N, Sakoda Y, Norimine J, Sekiguchi S
Journal of Veterinary Medical Science 2019年8月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Sudaryatma P., Mekata H., Kubo M., Subangkit M., Goto Y., Okabayashi T.
Veterinary Microbiology 235 80 - 85 2019年8月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Veterinary Microbiology
© 2019 Bovine respiratory disease complex is a major disease affecting the global cattle industry. Multiple infections by viruses and bacteria increase disease severity. Previously, we reported that bovine respiratory syncytial virus (BRSV) infection increases adherence of Pasteurella multocida to human respiratory and bovine kidney epithelial cells. To examine the interaction between the virus and bacteria in bovine respiratory cells, we generated respiratory epithelial cell lines from bovine trachea (bTEC), bronchus (bBEC), and lung (bLEC). Although all established cell lines were infected by BRSV and P. multocida susceptibility differed according to site of origin. The cells derived from the lower respiratory tract (bBEC and bLEC) were significantly more susceptible to BRSV than those derived from the upper respiratory tract (bTEC). Pre-infection of bBEC and bLEC with BRSV increased adherence of P. multocida; this was not the case for bTEC. These results indicate that BRSV may reproduce better in the lower respiratory tract and encourage adherence of bacteria. Thus, we identify one possible mechanism underlying severe pneumonia.
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Development of a LAMP assay for rapid and sensitive detection and differentiation of Mycobacterium avium subsp. avium and subsp. hominissuis. 査読あり
Yashiki N, Yamazaki Y, Subangkit M, Okabayashi T, Yamazaki W, Goto Y
Letters Appl. Microbiol. 2019年6月
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Genotyping of swine Mycobacterium avium subsp. hominissuis isolates from Kyushu, Japan. 査読あり
Subangkit M, Yamamoto T, Ishida M, Nomura A, Yasiki N, Sudaryatma PE, Goto Y, Okabayashi T
The Journal of veterinary medical science 2019年6月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：公益社団法人 日本獣医学会
The incidence of diseases caused by nontuberculous mycobacteria (NTM) is increasing annually worldwide, including Japan.<i> Mycobacterium avium </i>subsp.<i> hoiminissuis </i>(MAH) is one of the most common NTM species responsible for chronic lung diseases in animals and humans. In the current study, mycobacterial interspersed repetitive unit-variable number tandem repeat (MIRU-VNTR) typing was employed to characterize the genetic diversity of swine MAH isolates from Kyushu, Japan. In total, 309 isolates were obtained from the lymph nodes of 107 pigs not displaying any clinical signs of disease, of which 307 were identified as MAH, comprising 173 strains. Based on eight established MIRU-VNTR loci, the MAH strains represented 50 genotypes constituting three lineages, and 29 had not been described in the Mac French National Institute for Agricultural Research Nouzilly MIRU-VNTR (Mac-INVM) database. MAH was the dominant <i>M. avium</i> complex (MAC) in pigs from Kyushu, and there was high genetic diversity among genotype profiles of MAH from Kyushu. We identified three predominant genotype profiles in the tested area sharing high relatedness with genotype profiles of strains isolated in European countries. MAH was the most common NTM in pigs from Kyushu and exhibited high diversity, with new strain-derived genotypes.
DOI： 10.1292/jvms.19-0048
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Kosoltanapiwat N., Reamtong O., Okabayashi T., Ampawong S., Rungruengkitkun A., Thiangtrongjit T., Thippornchai N., Leaungwutiwong P., Mahittikorn A., Mori H., Yoohanngoa T., Yamwong P.
BMC Microbiology 18 ( 1 ) 135 2018年10月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：BMC Microbiology
© 2018 The Author(s). Background: The pteropine orthoreovirus (PRV) was isolated from monkey (Macaca fascicularis) faecal samples collected from human-inhabited areas in Lopburi Province, Thailand. These samples were initially obtained to survey for the presence of hepatitis E virus (HEV). Results: Two virus isolates were retrieved by virus culture of 55 monkey faecal samples. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was successfully used to identify the viruses as the segmented dsRNA orthoreovirus. Phylogenetic analysis of the Lopburi orthoreovirus whole-genomes revealed relationships with the well-characterised PRVs Pulau (segment L1), Cangyuan (segments L2, M3 and S3), Melaka (segments L3 and M2), Kampar (segments M1 and S2) and Sikamat (segments S1 and S4) of Southeast Asia and China with nucleotide sequence identities of 93.5-98.9%. RT-PCR showed that PRV was detected in 10.9% (6/55) and HEV was detected in 25.5% (14/55) of the monkey faecal samples. Conclusions: PRV was isolated from monkey faeces for the first time in Thailand via viral culture and LC-MS/MS. The genetic diversity of the virus genome segments suggested a re-assortment within the PRV species group. The overall findings emphasise that monkey faeces can be sources of zoonotic viruses, including PRV and HEV, and suggest the need for active virus surveillance in areas of human and monkey co-habitation to prevent and control emerging zoonotic diseases in the future.
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Koike N., Mai TN., Shirai M., Kubo M., Hata K., Marumoto N., Watanabe S., Sasaki Y., Mitoma S., Notsu K., Okabayashi T., Wiratsubaki A. ,Kabali E., Norimine J., Sekiguchi S.
Journal of Veterinary Medical Science 80 ( 11 ) 1782 - 1786 2018年10月
記述言語：英語 掲載種別：研究論文（学術雑誌）
The purpose of this study was to detect porcine epidemic diarrhea virus (PEDV) subclinically infected pigs shipped from non-case farms to slaughterhouses. Systematic sampling was conducted at two slaughterhouses. A total of 1,556 blood samples were collected from 80 case and non-case farms from pigs over 6 months old. Blood samples were centrifuged to obtain sera. Serial serum dilutions were subjected to serological examination for PEDV presence using Neutralization test (NT). The cut-off titer was set at titer of 1:2 dilution and farms with at least one positive sample in duplicate were classified as PED-positive farms. Several non-case farms (9.4%, 6/64) and 100% (16/16) of the case farms were indeed positive for PEDV. The proportion of seropositive animals from case farms was 63.7%, significantly different from that of non-case farms (4.3%, <i>P</i><0.05). In both case and non-case farms, the proportion of seropositive animals in farrow-to-finish farms was significantly higher than in wean-to-finish farms (<i>P</i><0.05). Seropositive animals in non-case farms were detected by NT in a sero-survey by sampling at slaughterhouses. Therefore, subclinically infected pigs should be considered prior to shipment.
DOI： 10.1292/jvms.18-0132
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Sudaryatma P., Nakamura K., Mekata H., Sekiguchi S., Kubo M., Kobayashi I., Subangkit M., Goto Y., Okabayashi T.
Veterinary Microbiology 220 33 - 38 2018年7月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Veterinary Microbiology
© 2018 Elsevier B.V. Primary infection with bovine respiratory syncytial virus (BRSV) predisposes cattle to secondary infection with bacteria that cause bovine respiratory disease complex (BRDC). However, the interaction between BRSV and bacteria is unclear. This in vitro study examined the adherence of Pasteurella multocida (PM) to BRSV-infected cells was assessed in colony forming unit assays, by flow cytometry analysis, and by indirect immunofluorescence analysis (IFA) of epithelial cells (A549, HEp-2, and MDBK). An in vitro model based on infection of BRSV-infected epithelial cells revealed that PM adherence to BRSV-infected cells was 2- to 8-fold higher than uninfected cells. This was confirmed by flow cytometry analysis and IFA. Epithelial cell expression of mRNA encoding cytokines and chemokines increased after exposure to PM, but increased further after co-infection with BRSV and PM. BRSV-mediated adherence of PM to epithelial cells may underlie the serious symptoms of BRDC.
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Mai T., Nguyen V., Yamazaki W., Okabayashi T., Mitoma S., Notsu K., Sakai Y., Yamaguchi R., Norimine J., Sekiguchi S.
BMC Veterinary Research 14 ( 1 ) 172 2018年5月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：BMC Veterinary Research
© 2018 The Author(s). Background: Porcine epidemic diarrhoea (PED) is an emerging disease in pigs that causes massive economic losses in the swine industry, with high mortality in suckling piglets. Early identification of PED virus (PEDV)-infected herd through surveillance or monitoring strategies is necessary for mass control of PED. However, a common working diagnosis system involves identifying PEDV-infected animals individually, which is a costly and time-consuming approach. Given the above information, the thrusts of this study were to develop a real-time fluorescent reverse transcription loop-mediated isothermal amplification (RtF-RT-LAMP) assay and establish a pooled testing system using faecal sample to identify PEDV-infected herd. Results: In this study, we developed an accurate, rapid, cost-effective, and simple RtF- RT-LAMP assay for detecting the PEDV genome targeting M gene. The pooled testing system using the RtF-RT-LAMP assay was optimized such that a pool of at least 15 individual faecal samples could be analysed. Conclusions: The developed RtF-RT-LAMP assay in our study could support the design and implementation of large-scaled epidemiological surveys as well as active surveillance and monitoring programs for effective control of PED.
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Jain J., Okabayashi T., Kaur N., Nakayama E., Shioda T., Gaind R., Kurosu T., Sunil S.
Virology Journal 15 ( 1 ) 84 2018年5月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Virology Journal
© 2018 The Author(s). Background: Chikungunya virus (CHIKV) and dengue virus (DENV) are arboviruses that share the same Aedes mosquito vector, and there is much overlap in endemic areas. In India, co-infection with both viruses is often reported. Clinical manifestations of Chikungunya fever is often confused with dengue fever because clinical symptoms of both infections are similar. It is, therefore, difficult to differentiate from those of other febrile illnesses, especially dengue fever. We previously developed a CHIKV antigen detection immunochromatography (IC) rapid diagnosis kit [1]. The current study examined the efficacy of previously mentioned IC kit in India, a dengue-endemic country. Methods: Sera from 104 CHIKV-positive (by qRT-PCR) and/or IgM-positive (ELISA) subjects collected in 2016, were examined. Fifteen samples from individuals with CHIKV-negative/DENV-positive and 4 samples from healthy individuals were also examined. Of the 104 CHIKV-positive sera, 20 were co-infected with DENV. Results: The sensitivity, specificity and overall agreement of the IC assay were 93.7, 95.5 and 94.3%, respectively, using qRT-PCR as a gold standard. Also, there was a strong, statistically significant positive correlation between the IC kit device score and the CHIKV RNA copy number. The IC kit detected CHIKV antigen even in DENV-co-infected patient sera and did not cross-react with DENV NS1-positive/CHIKV-negative samples. Conclusions: The results suggest that the IC kit is useful for rapid diagnosis of CHIKV in endemic areas in which both CHIKV and DENV are circulating.
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Molecular epidemiological survey and phylogenetic analysis of bovine influenza D virus in Japan 査読あり
Mekata H., Yamamoto M., Hamabe S., Tanaka H., Omatsu T., Mizutani T., Hause B., Okabayashi T.
Transboundary and Emerging Diseases 65 ( 2 ) e355 - e360 2018年4月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Transboundary and Emerging Diseases
© 2017 Blackwell Verlag GmbH The influenza D virus, a new member of the Orthomyxoviridae family, is predominantly found in cattle. Although viral pathology and clinical disease in cattle appear mild, this virus plays an important role as a trigger of bovine respiratory disease (BRD). BRD is a costly illness worldwide. Thus, epidemiological surveys of the influenza D virus are necessary. Here, we conducted a molecular epidemiological survey for the influenza D virus in healthy and respiratory-diseased cattle in Japan. We found that 2.1% (8/377) of the cattle were infected with influenza D. The cattle with and without respiratory symptoms had approximately equal amounts of the virus. A full-genome sequence analysis revealed that the influenza D virus that was isolated in Japan formed an individual cluster that was distinct from the strains found in other countries. These results suggest that this virus might have evolved uniquely in Japan over a long period of time and that the viral pathology of Japanese strains might be different from the strains found in other countries. Continuous surveillance is required to determine the importance of this virus and to characterize its evolution.
DOI： 10.1111/tbed.12765
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KOIKE Naoki, NOTSU Kosuke, OKABAYASHI Tamaki, WIRATSUDAKUL Anuwat, KABALI Emmanuel, NORIMINE Junzo, SEKIGUCHI Satoshi, MAI Thi Ngan, SHIRAI Mamoru, KUBO Meiko, HATA Kazuhiro, MARUMOTO Nobuyuki, WATANABE Shinji, SASAKI Yosuke, MITOMA Shuya
Ｔｈｅ Ｊｏｕｒｎａｌ ｏｆ Ｖｅｔｅｒｉｎａｒｙ Ｍｅｄｉｃａｌ Ｓｃｉｅｎｃｅ 80 ( 11 ) 1782 - 1786 2018年
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：公益社団法人 日本獣医学会
The purpose of this study was to detect porcine epidemic diarrhea virus (PEDV) subclinically infected pigs shipped from non-case farms to slaughterhouses. Systematic sampling was conducted at two slaughterhouses. A total of 1,556 blood samples were collected from 80 case and non-case farms from pigs over 6 months old. Blood samples were centrifuged to obtain sera. Serial serum dilutions were subjected to serological examination for PEDV presence using Neutralization test (NT). The cut-off titer was set at titer of 1:2 dilution and farms with at least one positive sample in duplicate were classified as PED-positive farms. Several non-case farms (9.4%, 6/64) and 100% (16/16) of the case farms were indeed positive for PEDV. The proportion of seropositive animals from case farms was 63.7%, significantly different from that of non-case farms (4.3%, <i>P</i><0.05). In both case and non-case farms, the proportion of seropositive animals in farrow-to-finish farms was significantly higher than in wean-to-finish farms (<i>P</i><0.05). Seropositive animals in non-case farms were detected by NT in a sero-survey by sampling at slaughterhouses. Therefore, subclinically infected pigs should be considered prior to shipment.
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Rahpaya S., Tsuchiaka S., Kishimoto M., Oba M., Katayama Y., Nunomura Y., Kokawa S., Kimura T., Kobayashi A., Kirino Y., Okabayashi T., Nonaka N., Mekata H., Aoki H., Shiokawa M., Umetsu M., Morita T., Hasebe A., Otsu K., Asai T., Yamaguchi T., Makino S., Murata Y., Abi A., Omatsu T., Mizutani T.
Journal of Veterinary Science 19 ( 3 ) 350 - 357 2018年
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Journal of Veterinary Science
© 2018 The Korean Society of Veterinary Science. Bovine abortion, diarrhea, and respiratory disease complexes, caused by infectious agents, result in high and significant economic losses for the cattle industry. These pathogens are likely transmitted by various vectors and reservoirs including insects, birds, and rodents. However, experimental data supporting this possibility are scarce. We collected 117 samples and screened them for 44 bovine abortive, diarrheal, and respiratory disease complex pathogens by using Dembo polymerase chain reaction (PCR), which is based on TaqMan real-time PCR. Fifty-seven samples were positive for at least one pathogen, including bovine viral diarrhea virus, bovine enterovirus, Salmonella enterica ser. Dublin, Salmonella enterica ser. Typhimurium, and Neospora caninum; some samples were positive for multiple pathogens. Bovine viral diarrhea virus and bovine enterovirus were the most frequently detected pathogens, especially in flies, suggesting an important role of flies in the transmission of these viruses. Additionally, we detected the N. caninum genome from a cockroach sample for the first time. Our data suggest that insects (particularly flies), birds, and rodents are potential vectors and reservoirs of abortion, diarrhea, and respiratory infectious agents, and that they may transmit more than one pathogen at the same time.
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Dengue Virus-Induced Reactive Oxygen Species Production in Rat Microglial Cells. 査読あり
Suwanprinya L, Phumala Morales N, Sanvarinda P, Dieng H, Okabayashi T, Enrique Morales Vargas R
Japanese journal of infectious diseases 70 ( 4 ) 383 - 387 2017年7月
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Diagnostic accuracy of a rapid E1-antigen test for chikungunya virus infection in a reference setting. 査読あり
Huits R, Okabayashi T, Cnops L, Barbé B, Van Den Berg R, Bartholomeeusen K, Ariën KK, Jacobs J, Bottieau E, Nakayama EE, Shioda T, Van Esbroeck M
Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases 2017年6月
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Sabike, I., Uemura, R., Kirino, Y., Mekata, H., Sekiguchi, S., Okabayashi, T., Goto, Y., Yamazaki, W.
Frontiers in Microbiology 7 ( SEP ) 1582 2016年9月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Frontiers in Microbiology
© 2016 Sabike, Uemura, Kirino, Mekata, Sekiguchi, Okabayashi, Goto and Yamazaki. Rapid identification of Campylobacter-positive flocks before slaughter, following freezing and heat treatment for the Campylobacter-positive carcasses at the slaughterhouses is an effective control strategy against foodborne campylobacteriosis. We evaluated a loop-mediated isothermal amplification (LAMP) assay for the direct screening of naturally contaminated chicken cloacal swabs for C. jejuni/C. coli to compare this assay with conventional qu antitative culture methods. In a comparison study of 165 broilers, the LAMP assay showed 82.8% (48/58 by conventional culture) sensitivity, 100% (107/107) specificity, 100% (48/48) positive predictive value (PPV), and 91.5% (107/117) negative predictive value (NPV). In a comparison of 55 flocks, LAMP showed 90.5% (19/21) sensitivity, 100% (34/34) specificity, 100% (19/19) PPV, and 94.4% (34/36) NPV. In the cumulative total of 28 farm-level comparisons, LAMP showed 100% (12/12) sensitivity, 100% (16/16) specificity, 100% (12/12) PPV, and 100% (16/16) NPV. The LAMP assay required less than 90 min from the arrival of the fecal samples to final results in the laboratory. This suggests that the LAMP assay will facilitate the identification of C. jejuni/C. coli-positive broiler flocks at the farm level or in slaughterhouses before slaughtering, which would make it an effective tool in preventing the spread of Campylobacter contamination.
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Dengue virus infection-enhancing antibody activities against Indonesian strains in inhabitants of central Thailand.
Yamanaka, A., Oddgun, D., Chantawat, N., Okabayashi, T., Ramasoota, P., Churrotin, S., Kotaki, T., Kameoka, M., Soegijanto, S., Konishi, E.
Microbes and infection 18 ( 4 ) 277 - 84 2016年4月
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Correction for Okabayashi et al., Detection of Chikungunya Virus Antigen by a Novel Rapid Immunochromatographic Test.
Okabayash,i T., Sasaki, T., Masrinoul, P., Chantawat, N., Yoksan, S., Nitatpattana, N., Chusri, S., Morales, Vargas. RE, Grandadam, M., Brey, PT., Soegijanto, S., Mulyantno, KC, Churrotin S, Kotaki, T., Faye, O., Faye, O., Sow, A., Sall, AA., Puiprom, O., Chaichana P., Kurosu, T., Kato, S., Kosaka, M., Ramasoota, P., Ikuta, K.
Journal of clinical microbiology 54 ( 4 ) 1173 - 4 2016年4月
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Detection of chikungunya virus antigen by a novel rapid immunochromatographic test.
Okabayashi T, Sasaki T, Masrinoul P, Chantawat N, Yoksan S, Nitatpattana N, Chusri S, Morales Vargas RE, Grandadam M, Brey PT, Soegijanto S, Mulyantno KC, Churrotin S, Kotaki T, Faye O, Faye O, Sow A, Sall AA, Puiprom O, Chaichana P, Kurosu T, Kato S, Kosaka M, Ramasoota P, Ikuta K
Journal of clinical microbiology 53 ( 2 ) 382 - 8 2015年2月
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Chikungunya virus was isolated in Thailand, 2010.
Sasayama M, Benjathummarak S, Kawashita N, Rukmanee P, Sangmukdanun S, Masrinoul P, Pitaksajjakul P, Puiprom O, Wuthisen P, Kurosu T, Chaichana P, Maneekan P, Ikuta K, Ramasoota P, Okabayashi T, Singhasivanon P, Luplertlop N
Virus genes 49 ( 3 ) 485 - 9 2014年12月
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Monoclonal antibody targeting chikungunya virus envelope 1 protein inhibits virus release.
Masrinoul P, Puiprom O, Tanaka A, Kuwahara M, Chaichana P, Ikuta K, Ramasoota P, Okabayashi T
Virology 464-465 111 - 7 2014年9月
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Antibody germline characterization of cross-neutralizing human IgGs against 4 serotypes of dengue virus.
Pitaksajjakul P, Benjathummarak S, Pipattanaboon C, Wongwit W, Okabayashi T, Kuhara M, Misaki R, Fujiyama K, Ramasoota P
Biochemical and biophysical research communications 446 ( 2 ) 475 - 80 2014年4月
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Low levels of antibody-dependent enhancement in vitro using viruses and plasma from dengue patients.
Chaichana P, Okabayashi T, Puiprom O, Sasayama M, Sasaki T, Yamashita A, Ramasoota P, Kurosu T, Ikuta K
PloS one 9 ( 3 ) e92173 2014年
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Marked induction of matrix metalloproteinase-10 by respiratory syncytial virus infection in human nasal epithelial cells.
Hirakawa S, Kojima T, Obata K, Okabayashi T, Yokota S, Nomura K, Obonai T, Fuchimoto J, Himi T, Tsutsumi H, Sawada N
Journal of medical virology 85 ( 12 ) 2141 - 50 2013年12月
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Humulone suppresses replication of respiratory syncytial virus and release of IL-8 and RANTES in normal human nasal epithelial cells.
Fuchimoto J, Kojima T, Okabayashi T, Masaki T, Ogasawara N, Obata K, Nomura K, Hirakawa S, Kobayashi N, Shigyo T, Yokota S, Fujii N, Tsutsumi H, Himi T, Sawada N
Medical molecular morphology 46 ( 4 ) 203 - 9 2013年12月
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Detection and characterization of enteric viruses in flood water from the 2011 Thai flood 査読あり
Ngaosuwankul N., Thippornchai N., Yamashita A., Vargas R., Tunyong W., Mahakunkijchareon Y., Ikuta K., Singhasivanon P., Okabayashi T., Leaungwutiwong P.
Japanese Journal of Infectious Diseases 66 ( 5 ) 398 - 403 2013年9月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Japanese Journal of Infectious Diseases
Severe flooding, which is associated with numerous outbreaks of a wide range of infectious diseases, particularly those caused by enteric viruses, occurred in all areas of Thailand in 2011. To determine the prevalence of five human enteric viruses, namely enterovirus, rotavirus (RV), norovirus (NV), hepatitis A virus (HAV), and hepatitis E virus, in the flood water, 100 water samples were collected from flood-damaged areas in central Thailand. Viral RNA was extracted from concentrated samples and analyzed by RT-PCR and sequencing. NV was the most commonly detected pathogen in the tested samples (14%). RV and HAV were detected in 9% and 7% of samples, respectively. This study is the first to detect enteric viral genes in flood water in Thailand. Furthermore, it is the first to detect an NV gene in any type of environmental water in Thailand. These results provide useful information for estimating the risk of flood waterborne viral infection.
DOI： 10.7883/yoken.66.398
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Characterization of chikungunya virus infection of a human keratinocyte cell line: role of mosquito salivary gland protein in suppressing the host immune response.
Puiprom O, Morales Vargas RE, Potiwat R, Chaichana P, Ikuta K, Ramasoota P, Okabayashi T
Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases 17 210 - 5 2013年7月
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Dengue virus neutralization and antibody-dependent enhancement activities of human monoclonal antibodies derived from dengue patients at acute phase of secondary infection.
Sasaki T, Setthapramote C, Kurosu T, Nishimura M, Asai A, Omokoko MD, Pipattanaboon C, Pitaksajjakul P, Limkittikul K, Subchareon A, Chaichana P, Okabayashi T, Hirai I, Leaungwutiwong P, Misaki R, Fujiyama K, Ono K, Okuno Y, Ramasoota P, Ikuta K
Antiviral research 98 ( 3 ) 423 - 31 2013年6月
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Detection and characterization of enteric viruses in flood water from the 2011 thai flood.
Ngaosuwankul N, Thippornchai N, Yamashita A, Vargas RE, Tunyong W, Mahakunkijchareon Y, Ikuta K, Singhasivanon P, Okabayashi T, Leaungwutiwong P
Japanese journal of infectious diseases 66 ( 5 ) 398 - 403 2013年
記述言語：日本語 掲載種別：研究論文（学術雑誌）
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Curcumin prevents replication of respiratory syncytial virus and the epithelial responses to it in human nasal epithelial cells.
Obata K, Kojima T, Masaki T, Okabayashi T, Yokota S, Hirakawa S, Nomura K, Takasawa A, Murata M, Tanaka S, Fuchimoto J, Fujii N, Tsutsumi H, Himi T, Sawada N
PloS one 8 ( 9 ) e70225 2013年
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Cross-reactivity of human monoclonal antibodies generated with peripheral blood lymphocytes from dengue patients with Japanese encephalitis virus.
Pipattanaboon C, Sasaki T, Nishimura M, Setthapramote C, Pitaksajjakul P, Leaungwutiwong P, Limkittikul K, Puiprom O, Sasayama M, Chaichana P, Okabayashi T, Kurosu T, Ono K, Ramasoota P, Ikuta K
Biologics : targets & therapy 7 175 - 87 2013年
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Imiquimod suppresses propagation of herpes simplex virus 1 by upregulation of cystatin A via the adenosine receptor A1 pathway.
Kan Y, Okabayashi T, Yokota S, Yamamoto S, Fujii N, Yamashita T
Journal of virology 86 ( 19 ) 10338 - 46 2012年10月
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Prevalence of fluoroquinolone-resistant Escherichia coli O25:H4-ST131 (CTX-M-15-nonproducing) strains isolated in Japan.
Yokota S, Sato T, Okubo T, Ohkoshi Y, Okabayashi T, Kuwahara O, Tamura Y, Fujii N
Chemotherapy 58 ( 1 ) 52 - 9 2012年
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Limited cross-reactivity of mouse monoclonal antibodies against Dengue virus capsid protein among four serotypes.
Noda M, Masrinoul P, Punkum C, Pipattanaboon C, Ramasoota P, Setthapramote C, Sasaki T, Sasayama M, Yamashita A, Kurosu T, Ikuta K, Okabayashi T
Biologics : targets & therapy 6 409 - 16 2012年
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Clarithromycin suppresses human respiratory syncytial virus infection-induced Streptococcus pneumoniae adhesion and cytokine production in a pulmonary epithelial cell line.
Yokota S, Okabayashi T, Hirakawa S, Tsutsumi H, Himi T, Fujii N
Mediators of inflammation 2012 528568 2012年
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Type-III interferon, not type-I, is the predominant interferon induced by respiratory viruses in nasal epithelial cells.
Okabayashi T, Kojima T, Masaki T, Yokota S, Imaizumi T, Tsutsumi H, Himi T, Fujii N, Sawada N
Virus research 160 ( 1-2 ) 360 - 6 2011年9月
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A nuclear factor-κB signaling pathway via protein kinase C δ regulates replication of respiratory syncytial virus in polarized normal human nasal epithelial cells.
Masaki T, Kojima T, Okabayashi T, Ogasawara N, Ohkuni T, Obata K, Takasawa A, Murata M, Tanaka S, Hirakawa S, Fuchimoto J, Ninomiya T, Fujii N, Tsutsumi H, Himi T, Sawada N
Molecular biology of the cell 22 ( 13 ) 2144 - 56 2011年7月
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Measles virus C protein suppresses gamma-activated factor formation and virus-induced cell growth arrest.
Yokota S, Okabayashi T, Fujii N
Virology 414 ( 1 ) 74 - 82 2011年5月
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Respiratory syncytial virus infection and the tight junctions of nasal epithelial cells.
Tsutsumi H, Kojima T, Hirakawa S, Masaki T, Okabayashi T, Yokota S, Fujii N, Himi T, Sawada N
Advances in oto-rhino-laryngology 72 153 - 6 2011年
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Fosfomycin suppresses RS-virus-induced Streptococcus pneumoniae and Haemophilus influenzae adhesion to respiratory epithelial cells via the platelet-activating factor receptor.
Yokota S, Okabayashi T, Yoto Y, Hori T, Tsutsumi H, Fujii N
FEMS microbiology letters 310 ( 1 ) 84 - 90 2010年9月
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Increased caspase-2 activity is associated with induction of apoptosis in IFN-beta sensitive melanoma cell lines.
Kamiya T, Okabayashi T, Yokota S, Kan Y, Ogino J, Yamashita T, Fujii N, Jimbow K
Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research 30 ( 5 ) 349 - 57 2010年5月
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Helicobacter pylori lipopolysaccharides upregulate toll-like receptor 4 expression and proliferation of gastric epithelial cells via the MEK1/2-ERK1/2 mitogen-activated protein kinase pathway.
Yokota S, Okabayashi T, Rehli M, Fujii N, Amano K
Infection and immunity 78 ( 1 ) 468 - 76 2010年1月
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The battle between virus and host: modulation of Toll-like receptor signaling pathways by virus infection.
Yokota S, Okabayashi T, Fujii N
Mediators of inflammation 2010 184328 2010年
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Efficacy of polarized hydroxyapatite and silk fibroin composite dressing gel on epidermal recovery from full-thickness skin wounds.
Okabayashi R, Nakamura M, Okabayashi T, Tanaka Y, Nagai A, Yamashita K
Journal of biomedical materials research. Part B, Applied biomaterials 90 ( 2 ) 641 - 6 2009年8月
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Fosfomycin suppresses chemokine induction in airway epithelial cells infected with respiratory syncytial virus.
Okabayashi T, Yokota S, Yoto Y, Tsutsumi H, Fujii N
Clinical and vaccine immunology : CVI 16 ( 6 ) 859 - 65 2009年6月
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Okabayashi R., Nakamura M., Okabayashi T., Tanaka Y., Nagai A., Yamashita K.
8th World Biomaterials Congress 2008 4 2008年12月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：8th World Biomaterials Congress 2008
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Occurrence of norovirus infections unrelated to norovirus outbreaks in an asymptomatic food handler population.
Okabayashi T, Yokota S, Ohkoshi Y, Ohuchi H, Yoshida Y, Kikuchi M, Yano K, Fujii N
Journal of clinical microbiology 46 ( 6 ) 1985 - 8 2008年6月
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[Molecular mechanisms for suppression of TLR signaling pathway by virus infection].
Fujii N, Yokota S, Yokosawa N, Okabayashi T
Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme 53 ( 6 ) 710 - 9 2008年5月
記述言語：日本語 掲載種別：研究論文（学術雑誌）
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Molecular mechanisms for suppression of TLR signaling pathway by virus infection
Fujii N., Yokota S., Yokosawa N., Okabayashi T.
Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme 53 ( 6 ) 710 - 719 2008年5月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme
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Measles virus P protein suppresses Toll-like receptor signal through up-regulation of ubiquitin-modifying enzyme A20.
Yokota S, Okabayashi T, Yokosawa N, Fujii N
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 22 ( 1 ) 74 - 83 2008年1月
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Suppression of NF-kappaB and AP-1 activation in monocytic cells persistently infected with measles virus.
Indoh T, Yokota S, Okabayashi T, Yokosawa N, Fujii N
Virology 361 ( 2 ) 294 - 303 2007年5月
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Single nucleotide polymorphisms and functional analysis of MxA promoter region in multiple sclerosis.
Furuyama H, Chiba S, Okabayashi T, Yokota S, Nonaka M, Imai T, Fujii N, Matsumoto H
Journal of the neurological sciences 249 ( 2 ) 153 - 7 2006年11月
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Cytokine regulation in SARS coronavirus infection compared to other respiratory virus infections.
Okabayashi T, Kariwa H, Yokota S, Iki S, Indoh T, Yokosawa N, Takashima I, Tsutsumi H, Fujii N
Journal of medical virology 78 ( 4 ) 417 - 24 2006年4月
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[Molecular mechanisms for suppression of interferon system by HSV-1 infection].
Fujii N, Yokota S, Yokosawa N, Okabayashi T
Nihon rinsho. Japanese journal of clinical medicine 64 Suppl 3 171 - 7 2006年3月
記述言語：日本語 掲載種別：研究論文（学術雑誌）
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Molecular mechanisms for suppression of interferon system by HSV-1 infection
Fujii N., Yokota S., Yokosawa N., Okabayashi T.
Nippon rinsho. Japanese journal of clinical medicine 64 Suppl 3 171 - 177 2006年3月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Nippon rinsho. Japanese journal of clinical medicine
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Serum-dependent expression of promyelocytic leukemia protein suppresses propagation of influenza virus.
Iki S, Yokota S, Okabayashi T, Yokosawa N, Nagata K, Fujii N
Virology 343 ( 1 ) 106 - 15 2005年12月
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Viral strategies for escape from host innate immune system
Yokota S., Yokosawa N., Okabayashi T., Fujii N.
Sapporo Medical Journal 74 ( 3-4 ) 17 - 22 2005年8月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Sapporo Medical Journal
In order to establish infection to host cells, viruses suppress or escape from the host immune response against microorganisms by various strategies. Interferon (IFN) system is an important contributor of innate immunity. IFN is induced by viral infection, and it promotes antiviral state through induction and/or activation of the effector molecules. Many viruses possess the suppression mechanisms for the anti-viral effector molecules, whereas they also perform inhibition of IFN signaling pathway, JAK/STAT pathway. We consider that latter is a most effective strategy counteracting IFN function, because the signaling pathway is an entrance of the system. The strategies counteracting JAK/STAT pathway are varied among virus species. Viruses perform (i) degradation of JAK/STAT components, (ii) suppression of activation (phosphorylation) of the components, (iii) inhibition of nuclear translocation of activated transcription factor, and (iv) induction of host JAK/STAT negative regulator. In this review, we describe details of these strategies, especially our recent results of HSV-1, measles virus and mumps virus.
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Induction of suppressor of cytokine signaling-3 by herpes simplex virus type 1 confers efficient viral replication.
Yokota S, Yokosawa N, Okabayashi T, Suzutani T, Fujii N
Virology 338 ( 1 ) 173 - 81 2005年7月
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Nuclear localization of Japanese encephalitis virus core protein enhances viral replication.
Mori Y, Okabayashi T, Yamashita T, Zhao Z, Wakita T, Yasui K, Hasebe F, Tadano M, Konishi E, Moriishi K, Matsuura Y
Journal of virology 79 ( 6 ) 3448 - 58 2005年3月
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[Molecular mechanisms for suppression of interferon signal transduction pathways caused by viral infections].
Fujii N, Yokota S, Yokosawa N, Okabayashi T
Uirusu 54 ( 2 ) 169 - 78 2004年12月
記述言語：日本語 掲載種別：研究論文（学術雑誌）
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Fujii N., Yokota S.I., Yokosawa N., Okabayashi T.
Uirusu. Journal of virology 54 ( 2 ) 169 - 178 2004年12月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Uirusu. Journal of virology
In order to establish infection to host cells, viruses suppress or escape from the host immune response against microorganisms by various strategies. Interferon (IFN) system is an important contributor of innate immunity. IFN is induced by viral infection, and it promotes antiviral state through induction and/or activation of the effector molecules. Many viruses possess the suppression or inhibition mechanisms for the anti-viral effector molecules, whereas they also perform inhibition of IFN signaling pathway, JAK/STAT pathway. We consider that latter is a most effective strategy counteracting IFN function, because the signaling pathway is an entrance of the system. The strategies counteracting JAK/STAT pathway are varied among virus species. Viruses perform (i) production of IFN-binding protein, (ii) degradation of JAK/STAT components, (iii) suppression of activation (phosphorylation) of the components, (iv) inhibition of nuclear translocation of activated transcription factor, and (v) induction of host JAK/STAT negative regulator. Here, we present these strategies, especially our recent resulta of HSV1, mumps virus, and measles virus. For example, HSV1 induces a host JAK/STAT negative regulator SOCS3 (suppressor of cytokine signaling-3). Mumps virus V protein promotes degradation of both STAT-1 and STAT-3. Measles virus freezes the flexibility of IFN-alpha receptor complex by the action of viral proteins, C and V.
DOI： 10.2222/jsv.54.169
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Induction of suppressor of cytokine signaling-3 by herpes simplex virus type 1 contributes to inhibition of the interferon signaling pathway.
Yokota S, Yokosawa N, Okabayashi T, Suzutani T, Miura S, Jimbow K, Fujii N
Journal of virology 78 ( 12 ) 6282 - 6 2004年6月
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Growth arrest of epithelial cells during measles virus infection is caused by upregulation of interferon regulatory factor 1.
Yokota S, Okabayashi T, Yokosawa N, Fujii N
Journal of virology 78 ( 9 ) 4591 - 8 2004年5月
記述言語：日本語 掲載種別：研究論文（学術雑誌）
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[Diversity of suppression mechanisms of JAK/STAT pathway by virus infection].
Yokota S, Yokosawa N, Okabayashi T, Fujii N
Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme 49 ( 4 ) 517 - 25 2004年3月
記述言語：日本語 掲載種別：研究論文（学術雑誌）
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Diversity of suppression mechanisms of JAK/STAT pathway by virus infection
Yokota S., Yokosawa N., Okabayashi T., Fujii N.
Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme 49 ( 4 ) 517 - 525 2004年3月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme
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Suppression of thermotolerance in mumps virus-infected cells is caused by lack of HSP27 induction contributed by STAT-1.
Yokota S, Yokosawa N, Kubota T, Okabayashi T, Arata S, Fujii N
The Journal of biological chemistry 278 ( 43 ) 41654 - 60 2003年10月
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Proteasome activator PA28gamma-dependent nuclear retention and degradation of hepatitis C virus core protein.
Moriishi K, Okabayashi T, Nakai K, Moriya K, Koike K, Murata S, Chiba T, Tanaka K, Suzuki R, Suzuki T, Miyamura T, Matsuura Y
Journal of virology 77 ( 19 ) 10237 - 49 2003年10月
記述言語：日本語 掲載種別：研究論文（学術雑誌）
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Moriishi K., Okabayashi T., Nakai K., Moriya K., Koike K., Murata S., Chiba T., Tanaka K., Suzuki R., Suzuki T., Miyamura T., Matsuura Y.
Journal of Virology 77 ( 19 ) 10237 - 10249 2003年10月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Journal of Virology
Hepatitis C virus (HCV) core protein plays an important role in the formation of the viral nucleocapsid and a regulatory protein involved in hepatocarcinogenesis. In this study, we have identified proteasome activator PA28γ (11S regulator γ) as an HCV core binding protein by using yeast two-hybrid system. This interaction was demonstrated not only in cell culture but also in the livers of HCV core transgenic mice. These findings are extended to human HCV infection by the observation of this interaction in liver specimens from a patient with chronic HCV infection. Neither the interaction of HCV core protein with other PA28 subtypes nor that of PA28γ with other Flavivirus core proteins was detected. Deletion of the PA28γ-binding region from the HCV core protein or knockout of the PA28γ gene led to the export of the HCV core protein from the nucleus to the cytoplasm. Overexpression of PA28γ enhanced the proteolysis of the HCV core protein. Thus, the nuclear retention and stability of the HCV core protein is regulated via a PA28γ-dependent pathway through which HCV pathogenesis may be exerted.
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Detection of Babesia microti-like parasite in filter paper-absorbed blood of wild rodents.
Okabayashi T, Hagiya J, Tsuji M, Ishihara C, Satoh H, Morita C
The Journal of veterinary medical science 64 ( 2 ) 145 - 7 2002年2月
記述言語：日本語 掲載種別：研究論文（学術雑誌）
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Satoh H., Tsuneki A., Inokuma H., Kumazawa N., Jahana Y., Kiyuuna T., Okabayashi T., Muramatsu Y., Ueno H., Morita C.
Microbiology and Immunology 45 ( 1 ) 85 - 87 2001年
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Microbiology and Immunology
We evaluated serum antibodies against Rickettsia japonica in 517 dogs (430 stray dogs and 87 pet dogs) and 164 humans in Okinawa, Japan, by indirect immunofluorescence assay. The seropositive rate in stray dogs was significantly higher than that in pet dogs (30.7 versus 4.6%, P<0.01). This high prevalence rate is attributed to the understandably frequent environmental exposure of stray dogs to tick infestation. Human samples obtained from Okinawa and Sapporo also showed a significant difference in seropositive antibody percentages (45.1 and 12.0%, respectively, P<0.01). This result suggests that there has been pre-exposure to spotted fever group rickettsia in humans in Okinawa.
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Prevalence of spotted fever Rickettsial antibodies in dogs and rodents in the Philippines
Camer G.A., Masangkay J., Satoh H., Okabayashi T., Norizuki S., Motoi Y., Ueno H., Morita C.
Japanese Journal of Infectious Diseases 53 ( 4 ) 162 - 163 2000年12月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Japanese Journal of Infectious Diseases
Antibodies against spotted fever group rickettsiae have been detected in blood samples of dogs and rodents obtained from selected areas in the Philippines. In this serosurvey, the positive percentage rates are 8.3% (11/132) in dogs and 12.2% (6/49) in rats. Positive results were read from samples tested with Rickettsia japonica antigen. No positive result was obtained in blood samples of rats and house mice using R. akari antigen. The findings of this study are the first to confirm the detection of spotted fever group rickettsial antibodies in the Philippines.
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Okabayashi T., Hasebe F., Samui K., Mweene A., Pandey S., Yanase T., Muramatsu Y., Ueno H., Morita C.
American Journal of Tropical Medicine and Hygiene 61 ( 1 ) 70 - 72 1999年7月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：American Journal of Tropical Medicine and Hygiene
The causative agents of rickettsial diseases (Rickettsia conorii, R. typhi, and Coxiella burnetii) have been reported throughout the African continent. However, there have been no reports on epidemiologic surveys of these infections in Zambia. This study was designed to clarify the prevalence of three rickettsioses in 377 humans in Zambia. The seroprevalence of antibodies against R. conorii, R. typhi, and C. burnetii was 16.7%, 5.0%, and 8.2%, respectively. The rates of antibody positivity against R. conorii and C. burnetii were higher in the eastern (23.1% and 11.8%) and western (16.8% and 7.4%) areas of Zambia than in the northern (3.0% and 3.0%) area of this country. There was little difference among the three areas in the distribution of antibodies against R. typhi. Since cattle breeding is more extensive in the western and eastern areas than in the northern area, it is thought that cattle-breeding areas are foci of R. conorii and C. burnetii infections in Zambia.
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Ibrahim I., Okabayashi T., Ristiyanto, Lestari E., Yanase T., Muramatsu Y., Ueno H., Morita C.
European Journal of Epidemiology 15 ( 1 ) 89 - 93 1999年3月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：European Journal of Epidemiology
The prevalence of antibodies against spotted fever group rickettsia (SFGR), murine typhus and Q fever were investigated in wild rats captured in Indonesia. Sera of 327 rats were collected from Jakarta and Boyolali on Java Island. The prevalences of antibodies against SFGR and murine typhus were 128 (39.1%) and 48 (14.7%), respectively. Antibodies against Q fever were not detected in these serum samples. Antibodies against SFGR were found in all species of rats (20.8-51.9%). The antibody positive rate against murine typhus in Rattus norvegicus (38.0%) was significantly higher than that in other rat species (0-4.8%,p < 0.01). The antibody positive rates against SFGR and murine typhus in rats captured in Jakarta were significantly higher than those in rats captured in Boyolali (p < 0.01). In this survey, all species of rats had antibodies against SFGR, indicating that the 4 species of tested rats (R. norvegicus, R. rattus, R. exulans, R. tiomanicus) were infected with SFGR and that SFGR may infest the whole of Java Island. Most of the rats that were antibody-positive against murine typhus were captured in Jakarta. Therefore, R. norvegicus and R. rattus are likely to be important hosts of murine typhus in Jakarta. The antibody-positive rates against SFGR and murine typhus in rats captured in the dry season were significantly higher than those in rats captured in the rainy season. This may coincide with the active periods of ticks and fleas in Indonesia.
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Detection of Coxiella burnetii from dust in a barn housing dairy cattle 査読あり
Yanase T., Muramatsu Y., Inouye I., Okabayashi T., Ueno H., Morita C.
Microbiology and Immunology 42 ( 1 ) 51 - 53 1998年
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Microbiology and Immunology
We attempted to detect Coxiella burnetii in dust samples collected from a barn housing dairy cattle by the polymerase chain reaction (PCR) method. Ten dust samples (five from ventilation fans and five from crossbeams) were collected from two areas in a barn on a farm near Sapporo, Hokkaido. C. burnetii was detected in 5 of the 10 dust samples. It was believed that aerial contamination by C. burnetii occurred in the barn.
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Muramatsu Y., Yanase T., Okabayashi T., Ueno H., Morita C.
Applied and Environmental Microbiology 63 ( 6 ) 2142 - 2146 1997年6月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Applied and Environmental Microbiology
The use of an adequate concentration of Triton X-100 enhanced immunomagnetic separation of Coxiella burnetii from milk. PCR-enzyme-linked immunosorbent assay (PCR-ELISA) could detect coxiellas more sensitively than could conventional PCR. PCR-ELISA is therefore thought to be suitable for the simultaneous assay of a large number of samples. However, the number of cows from which raw milk tested positive for coxiellas by PCR-ELISA was inconsistent with that found with the antibody to coxiella by indirect immunofluorescence assay. The inconsistency is thought to be associated with the differences in the infectious route, infectious dose, or the timing of yielding the antibody and the period of duration of the antibody.
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Serological Survey of Spotted Fever Group Rickettsia in Wild Rats in Thailand in the 1970s
Okabayashi Tamaki, Tsutiya Kimiyuki, Muramatsu Yasukazu, UENO Hiroshi, MORITA Chiharu
Microbiology and immunology 40 ( 12 ) 895 - 898 1996年12月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Center for Academic Pub. Japan
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Serological survey of spotted fever group rickettsia in wild rats in thailand in the 1970s
Okabayashi T., Tsutiya K., Muramatsu Y., Ueno H., Morita C.
Microbiology and Immunology 40 ( 12 ) 895 - 898 1996年1月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Microbiology and Immunology
In Thailand, the first human cases of spotted fever group rickettsiosis were reported in 1994, but no serosurveys on wild rats have yet been conducted. We investigated the seroepidemiology in wild rats collected in the 1970s from two regions in Thailand, and found a 62.2% positive rate of antibodies for spotted fever group rickettsia (SFGR) by the indirect immunofluorescence antibody test. Of the antibody-positive rats, 82.2% had higher titers of antibodies against TT-118 than those against Rickettsia japonica, which suggests that Thailand is infested mainly with the TT-118 strain or its antigenically related organisms. The prevalence of antibodies in Bandicota indica was significantly higher than that in other species, which suggests that B. indica is important as a reservoir of SFGR in Thailand.
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Serological Survey of Spotted Fever Group Rickettsia in Wild Rats in Thailand in the 1970s
Okabayashi Tamaki, Tsutiya Kimiyuki, Muramatsu Yasukazu, Ueno Hiroshi, Morita Chiharu
MICROBIOLOGY and IMMUNOLOGY 40 ( 12 ) 895 - 898 1996年
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Center For Academic Publications Japan
In Thailand, the first human cases of spotted fever group rickettsiosis were reported in 1994, but no serosurveys on wild rats have yet been conducted. We investigated the seroepidemiology in wild rats collected in the 1970s from two regions in Thailand, and found a 62.2% positive rate of antibodies for spotted fever group rickettsia (SFGR) by the indirect immunofluorescence antibody test. Of the antibody-positive rats, 82.2% had higher titers of antibodies against TT-118 than those against <i>Rickettsia japonica</i>, which suggests that Thailand is infested mainly with the TT-118 strain or its antigenically related organisms. The prevalence of antibodies in <i>Bandicota indica</i> was significantly higher than that in other species, which suggests that <i>B. indica</i> is important as a reservoir of SFGR in Thailand.
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Epidemiological survey of small rodents for spotted fever rickettsial antibody in Hokkaido, Japan
Okabayashi T., Miura N., Miyazaki S., Nigo T., Muramatsu Y., Ueno H., Morita C.
Japanese Journal of Medical Science and Biology 49 ( 2 ) 63 - 68 1996年
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Japanese Journal of Medical Science and Biology
The prevalence of antibody against spotted fever group rickettsia (SFGR) was investigated with Rickettsia japonica antigen in small rodents captured in an area in Hokkaido, where no human cases of SFGR infection had been reported. Antibody against SFGR was found in Apodemus speciosus and Clethrionomys rufocanus but not in Apodemus argenteus. None of these rodents had antibody against R. typhi. This is the first report of the antibody against SFGR in rodents in Hokkaido.