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Affiliation |
Faculty of Medicine School of Medicine Department of Anatomy, Histochemistry and Cell Biology |
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Assistant Professor |
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Related SDGs |
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ISHIZUKA Takumi
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Research Areas 【 display / non-display 】
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Nanotechnology/Materials / Bio chemistry
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Nanotechnology/Materials / Chemical biology
Education 【 display / non-display 】
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The University of Tokyo Graduate School, Division of Engineering
- 2011.3
Country：Japan
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Tokyo Institute of Technology Faculty of Life Science and Engineering
- 2006.3
Country：Japan
Papers 【 display / non-display 】
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Fidya, Ishizuka Takumi, Kubota Toshiki, Kai Kengo, Hishikawa Yoshitaka
ACTA HISTOCHEMICA ET CYTOCHEMICA 59 ( 2 ) 89 - 99 2026.4
Language：English Publishing type：Research paper (scientific journal) Publisher：JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY
Periodontitis is a chronic inflammatory disease characterized by collagen degradation and alveolar bone loss. Although estrogen contributes to periodontal homeostasis, the role of the membrane-bound G protein–coupled estrogen receptor 30 (GPR30) in periodontitis remains unclear. This study investigated the temporal involvement of GPR30 in ligature-induced periodontitis, focusing on alveolar bone changes, collagen integrity, fibroblast activation, and epigenetic regulation. Twenty male Wistar rats were allocated to control and periodontitis groups and evaluated at days 7, 14, and 21 following placements of a stainless-steel ligature around the maxillary first molars. Alveolar bone loss was assessed radiographically, while inflammatory changes and collagen organization were examined using hematoxylin–eosin and Masson’s trichrome staining. Immunohistochemistry was performed to evaluate GPR30, collagen I/III, α-smooth muscle actin (α-SMA), and histone modifications. Statistical analysis was conducted using one-way ANOVA with Tukey’s post hoc test. Alveolar bone loss and collagen degradation were detectable on day 7 and peaked at day 14. GPR30 expression increased during the active inflammatory phase, accompanied by elevated α-SMA and histone H4 lysine 8 crotonylation (H4K8cr), and declined by day 21. Fibroblast transiently adopted a myofibroblast phenotype during inflammation and regressed during early tissue repair. Temporal changes in H4K8cr closely paralleled GPR30 expression. These findings indicate that GPR30 is dynamically associated with inflammatory and remodeling phases of periodontitis and may contribute to epigenetic regulation during periodontal tissue remodeling.
DOI： 10.1267/ahc.26-00002
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Effects of Fluorine Substitution on the Human Telomeric RNA G-Quadruplex Structure Reviewed
Saneyoshi H., Ishizuka T., Wang S., Iwakiri R., Xu Y.
Chemistry A European Journal 31 ( 39 ) 2025.7
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal) Publisher：Chemistry A European Journal
DNA G-quadruplex structures can adopt different topologies, whereas RNA G4 predominantly forms parallel-type structures. While previous studies have reported the stabilization of DNA G-quadruplex topologies using syn-favored nucleosides, stabilization of parallel-type RNA G-quadruplexes through chemical modification remains unachieved. Here, we demonstrate that substitution with 2′-deoxy-2′-fluoroarabinoguanosine (2′F-araG) at specific positions in the human telomeric RNA sequence stabilizes the parallel-type G-quadruplex structure. This stabilization arises from electrostatic interactions via pseudo hydrogen bonds, induced by the 2′F-araG modification.
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Kai K, Ishizuka T, Matsumoto J, Shimamawari K, Mori R, Fidya, Lkham-Erdene B, Kubota T, Ikenoue M, Higuchi K, Nanashima A, Hishikawa Y
Acta Histochemica et Cytochemica 58 ( 2 ) 69 - 79 2025.4
Language：English Publishing type：Research paper (scientific journal)
DOI： 10.1267/ahc.25-00002
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Higuchi K, Ikenoue M, Ishizuka T, Kai K, Takahashi N, Kubota T, Shirouzu S, Lkham-Erdene B, Mo Aung K, Nakai M, Sawaguchi A, Nanashima A, Hishikawa Y
Acta Histochemica et Cytochemica 58 ( 1 ) 9 - 18 2025.2
Language：English Publishing type：Research paper (scientific journal)
DOI： 10.1267/ahc.24-00061
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Lkham-Erdene B., Choijookhuu N., Kubota T., Uto T., Mitoma S., Shirouzu S., Ishizuka T., Kai K., Higuchi K., Aung K.M., Batmunkh J.E., Sato K., Hishikawa Y.
Acta Histochemica et Cytochemica 57 ( 5 ) 175 - 188 2024.10
Language：English Publishing type：Research paper (scientific journal) Publisher：Acta Histochemica et Cytochemica
Metabolic dysfunction-associated steatotic liver disease (MASLD) is becoming a major health problem worldwide. Liver regeneration is crucial for restoring liver function, and is regulated by extraordinary complex process, involving numerous factors under both physiologic and pathologic conditions. Sphingosine-1-phosphate (S1P), a bioactive sphingolipid synthesized by sphingosine kinase 1 (SphK1), plays an important role in liver function through S1P receptors (S1PRs)-expressing cells. In this study, we investigated the effect of lipid overload on hepatocyte proliferation in a mouse hepatic steatosis model induced by feeding a methionine-and choline-deficient (MCD) diet. After 50% partial hepatectomy (PHx), liver tissues were sampled at various timepoints and then analyzed by immunohistochemistry, oil Red-O staining, quantitative-polymerase chain reaction (qPCR), and flow cytometry. In mice fed the MCD-diet, significantly exacerbated hepatic steatosis and accelerated liver regeneration were observed. After PHx, hepatocyte proliferation peaked at 48 and 36 hr in the liver of chow-and MCD-diet fed mice, respectively. By contrast, increased expression of S1PR2 was observed in hepatic neutrophils and macrophages of MCD-diet fed mice. Flow cytome-try and qPCR experiments demonstrated that levels of HGF and FGF2 released by neutrophils and macrophages were significantly higher in MCD-diet fed mice. In conclusion, hepatic lipid overload recruits Kupffer cells and neutrophils that release HGF and FGF2 via SphK1/S1PR2 activation to accelerate hepatocyte proliferation.
DOI： 10.1267/ahc.24-00046
Books 【 display / non-display 】
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In situ hybridization法
菱川 善隆, 石塚 匠, 柴田 恭明, 小路 武彦（ Role： Joint author）
日本組織細胞化学会・中西印刷（株） 2025.7
Language：Japanese Book type：Scholarly book
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In situ hybridization法
菱川 善隆, 石塚 匠, 柴田 恭明, 小路 武彦（ Role： Joint author）
日本組織細胞化学会・中西印刷（株） 2024.7
Language：Japanese Book type：Scholarly book
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蛍光共鳴エネルギー移動（FRET）現象を利用した 高感度in situ hybridization
石塚 匠，Narantsog Choijookhuu，柴田 恭明，小路 武彦，菱川 善隆（ Role： Joint author）
日本顕微鏡学会 2023.12
Language：Japanese Book type：Scholarly book
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In situ hybridization法
菱川 善隆, Narantsog Choijookhuu, 石塚 匠, 柴田 恭明, 小路 武彦（ Role： Joint author）
日本組織細胞化学会・中西印刷（株） 2023.7
Language：Japanese Book type：Scholarly book
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In situ hybridization法
菱川 善隆, Narantsog Choijookhuu, 石塚 匠, 柴田 恭明, 小路 武彦（ Role： Joint author）
日本組織細胞化学会・中西印刷（株） 2022.7
Language：Japanese Book type：Scholarly book
Presentations 【 display / non-display 】
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マウス肝再生過程におけるDNA四重鎖構造の時空間的形成
石塚 匠, Kham Mo Aung, 久保田 壽樹, Lkham-Erdene Baljinnyam, 甲斐 健吾, Phyu Synn Oo, 菱川 善隆
第131回 日本解剖学会総会・全国学術集会 (東京慈恵会医科大学) 2026.3.24
Event date： 2026.3.24 - 2026.3.26
Language：Japanese Presentation type：Oral presentation (general)
Venue：東京慈恵会医科大学
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DNA G-quadruplex dynamics during liver regeneration after partial hepatectomy in mice International conference
Takumi Ishizuka, Kham Mo Aung, Toshiki Kubota, Baljinnyam Lkham-Erdene, Kengo Kai, Phyu Synn Oo, Yoshitaka Hishikawa
第52回国際核酸化学シンポジウム/日本核酸化学会第9回年会 (富山国際会議場) 2025.11.12 日本核酸化学会
Event date： 2025.11.12 - 2025.11.14
Language：English Presentation type：Poster presentation
Venue：富山国際会議場
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蛍光共鳴エネルギー移動（FRET）を応用したモレキュラービーコンプローブによるin situ hybridization Invited
Narantsog Choijookhuu，柴田 恭明，石塚 匠，徐 岩，小路 武彦，菱川 善隆
第66回日本組織細胞化学会総会・学術集会 (大阪公立大学 I-siteなんば) 2025.10.25
Event date： 2025.10.25 - 2025.10.26
Language：Japanese Presentation type：Oral presentation (invited, special)
Venue：大阪公立大学 I-siteなんば
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超解像顕微鏡による生体分子蛍光イメージング解析 Invited
石塚 匠
第66回日本組織細胞化学会総会・学術集会 (大阪公立大学 I-siteなんば) 2025.10.25
Event date： 2025.10.25 - 2025.10.26
Language：Japanese Presentation type：Symposium, workshop panel (nominated)
Venue：大阪公立大学 I-siteなんば
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Biological role of DNA G-quadruplex during liver regeneration after partial hepatectomy International conference
Takumi Ishizuka, Kham Mo Aung, Toshiki Kubota, Baljinnyam Lkham-Erdene, Kengo Kai, Yoshitaka Hishikawa
17th international congress of histochemistry and cytochemistry (Rimini, Italy)
Event date： 2025.8.27 - 2025.8.30
Language：English Presentation type：Poster presentation
Venue：Rimini, Italy Country：Italy
Awards 【 display / non-display 】
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日本組織細胞化学会 Paul K. Nakane AHC Award (Gold medal)
2025.10 日本組織細胞化学会
Narantsog Choijookhuu, Yasuaki Shibata, Takumi Ishizuka, Yan Xu, Takehiko Koji, Yoshitaka Hishikawa
Award type：International academic award (Japan or overseas)
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日本組織細胞化学会 論文賞
2023.10 日本組織細胞化学会
Narantsog Choijookhuu, Yasuaki Shibata, Takumi Ishizuka, Yan Xu, Takehiko Koji, Yoshitaka Hishikawa
Award type：International academic award (Japan or overseas)
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FB3 優秀ポスター賞
2012.7 FB3 organizing committee
石塚匠
Award type：Award from international society, conference, symposium, etc. Country：Sweden
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東京大学大学院工学系研究科長賞（修士）
2008.3 東京大学
石塚匠
Country：Japan
Grant-in-Aid for Scientific Research 【 display / non-display 】
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精子形成過程から解明するグアニン四重鎖構造の生物学的機能
Grant number：24K08641 2024.04 - 2027.03
独立行政法人日本学術振興会 科学研究費基金 基盤研究(C)
Authorship：Principal investigator
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PDP効果に基づく胃癌腹膜播種に対する簡便かつ安全な光治療の開発
Grant number：24K11937 2024.04 - 2027.03
独立行政法人日本学術振興会 科学研究費基金 基盤研究(C)
Authorship：Coinvestigator(s)
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細胞内RNA四重鎖を標的とする構造プローブ法の開発
Grant number：21K05314 2021.04 - 2024.03
独立行政法人日本学術振興会 科学研究費補助金 基盤研究(C)
Authorship：Principal investigator
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分子結合技術を用いた新たな造影剤による革新的がんMRI画像化技術の開発
Grant number：20K08000 2020.04 - 2023.03
独立行政法人日本学術振興会 科学研究費補助金 基盤研究(C)
Authorship：Coinvestigator(s)
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分子結合技術を用いた造影剤による革新的がんCT画像化技術の開発
Grant number：19K08156 2019.04 - 2022.03
科学研究費補助金 基盤研究(C)
Authorship：Coinvestigator(s)
Other research activities 【 display / non-display 】
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第47回組織細胞化学講習会
2022.08
「In situ hybridization法」の技術講習会Wet Labを行った。
Social Activities 【 display / non-display 】
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解剖見学及び標本示説実習（鵬翔高等学校看護専攻科）
Role(s)： Lecturer
2023.2.1 - 2023.2.2
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解剖見学及び標本示説実習（九州保健福祉大学総合医療専門学校）
Role(s)： Lecturer
2023.1.31
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解剖見学及び標本示説実習（宮崎リハビリテーション学院）
Role(s)： Lecturer
2023.1.23
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解剖見学及び標本示説実習（小林看護医療専門学校）
Role(s)： Lecturer
2023.1.11
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解剖見学及び標本示説実習（宮崎看護専門学校）
Role(s)： Lecturer
2022.9.2