|
Affiliation |
Faculty of Medicine School of Medicine Department of Anatomy, Histochemistry and Cell Biology |
|
Title |
Assistant Professor |
|
External Link |
|
|
Related SDGs |
|
ISHIZUKA Takumi
|
|
|
Research Areas 【 display / non-display 】
-
Nanotechnology/Materials / Bio chemistry
-
Nanotechnology/Materials / Chemical biology
Education 【 display / non-display 】
-
The University of Tokyo Graduate School, Division of Engineering
- 2011.3
Country：Japan
-
Tokyo Institute of Technology Faculty of Life Science and Engineering
- 2006.3
Country：Japan
Papers 【 display / non-display 】
-
Effects of Fluorine Substitution on the Human Telomeric RNA G-Quadruplex Structure Reviewed
Saneyoshi H., Ishizuka T., Wang S., Iwakiri R., Xu Y.
Chemistry A European Journal 31 ( 39 ) 2025.7
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal) Publisher：Chemistry A European Journal
DNA G-quadruplex structures can adopt different topologies, whereas RNA G4 predominantly forms parallel-type structures. While previous studies have reported the stabilization of DNA G-quadruplex topologies using syn-favored nucleosides, stabilization of parallel-type RNA G-quadruplexes through chemical modification remains unachieved. Here, we demonstrate that substitution with 2′-deoxy-2′-fluoroarabinoguanosine (2′F-araG) at specific positions in the human telomeric RNA sequence stabilizes the parallel-type G-quadruplex structure. This stabilization arises from electrostatic interactions via pseudo hydrogen bonds, induced by the 2′F-araG modification.
-
Kai K, Ishizuka T, Matsumoto J, Shimamawari K, Mori R, Fidya, Lkham-Erdene B, Kubota T, Ikenoue M, Higuchi K, Nanashima A, Hishikawa Y
Acta Histochemica et Cytochemica 58 ( 2 ) 69 - 79 2025.4
Language：English Publishing type：Research paper (scientific journal)
DOI： 10.1267/ahc.25-00002
-
Higuchi K, Ikenoue M, Ishizuka T, Kai K, Takahashi N, Kubota T, Shirouzu S, Lkham-Erdene B, Mo Aung K, Nakai M, Sawaguchi A, Nanashima A, Hishikawa Y
Acta Histochemica et Cytochemica 58 ( 1 ) 9 - 18 2025.2
Language：English Publishing type：Research paper (scientific journal)
DOI： 10.1267/ahc.24-00061
-
Lkham-Erdene B., Choijookhuu N., Kubota T., Uto T., Mitoma S., Shirouzu S., Ishizuka T., Kai K., Higuchi K., Aung K.M., Batmunkh J.E., Sato K., Hishikawa Y.
Acta Histochemica et Cytochemica 57 ( 5 ) 175 - 188 2024.10
Language：English Publishing type：Research paper (scientific journal) Publisher：Acta Histochemica et Cytochemica
Metabolic dysfunction-associated steatotic liver disease (MASLD) is becoming a major health problem worldwide. Liver regeneration is crucial for restoring liver function, and is regulated by extraordinary complex process, involving numerous factors under both physiologic and pathologic conditions. Sphingosine-1-phosphate (S1P), a bioactive sphingolipid synthesized by sphingosine kinase 1 (SphK1), plays an important role in liver function through S1P receptors (S1PRs)-expressing cells. In this study, we investigated the effect of lipid overload on hepatocyte proliferation in a mouse hepatic steatosis model induced by feeding a methionine-and choline-deficient (MCD) diet. After 50% partial hepatectomy (PHx), liver tissues were sampled at various timepoints and then analyzed by immunohistochemistry, oil Red-O staining, quantitative-polymerase chain reaction (qPCR), and flow cytometry. In mice fed the MCD-diet, significantly exacerbated hepatic steatosis and accelerated liver regeneration were observed. After PHx, hepatocyte proliferation peaked at 48 and 36 hr in the liver of chow-and MCD-diet fed mice, respectively. By contrast, increased expression of S1PR2 was observed in hepatic neutrophils and macrophages of MCD-diet fed mice. Flow cytome-try and qPCR experiments demonstrated that levels of HGF and FGF2 released by neutrophils and macrophages were significantly higher in MCD-diet fed mice. In conclusion, hepatic lipid overload recruits Kupffer cells and neutrophils that release HGF and FGF2 via SphK1/S1PR2 activation to accelerate hepatocyte proliferation.
DOI： 10.1267/ahc.24-00046
-
Ikenoue M., Choijookhuu N., Yano K., Fidya , Takahashi N., Ishizuka T., Shirouzu S., Yamaguma Y., Kai K., Higuchi K., Sawaguchi A., Nanashima A., Hishikawa Y.
Histochemistry and Cell Biology 161 ( 4 ) 325 - 336 2024.4
Language：English Publishing type：Research paper (scientific journal) Publisher：Histochemistry and Cell Biology
Su (var) 3–9, enhancer of seste, trithorax (SET)-domain bifurcated histone lysine methyltransferase (SETDB1) plays a crucial role in maintaining intestinal stem cell homeostasis; however, its physiological function in epithelial injury is largely unknown. In this study, we investigated the role of SETDB1 in epithelial regeneration using an intestinal ischemia/reperfusion injury (IRI) mouse model. Jejunum tissues were sampled after 75 min of ischemia followed by 3, 24, and 48 h of reperfusion. Morphological evaluations were performed using light microscopy and electron microscopy, and the involvement of SETDB1 in epithelial remodeling was investigated by immunohistochemistry. Expression of SETDB1 was increased following 24 h of reperfusion and localized in not only the crypt bottom but also in the transit amplifying zone and part of the villi. Changes in cell lineage, repression of cell adhesion molecule expression, and decreased histone H3 methylation status were detected in the crypts at the same time. Electron microscopy also revealed aberrant alignment of crypt nuclei and fusion of adjacent villi. Furthermore, increased SETDB1 expression and epithelial remodeling were confirmed with loss of stem cells, suggesting SETDB1 affects epithelial cell plasticity. In addition, crypt elongation and increased numbers of Ki-67 positive cells indicated active cell proliferation after IRI; however, the expression of PCNA was decreased compared to sham mouse jejunum. These morphological changes and the aberrant expression of proliferation markers were prevented by sinefungin, a histone methyltransferase inhibitor. In summary, SETDB1 plays a crucial role in changes in the epithelial structure after IRI-induced stem cell loss.
Books 【 display / non-display 】
-
In situ hybridization法
菱川 善隆, 石塚 匠, 柴田 恭明, 小路 武彦（ Role： Joint author）
日本組織細胞化学会・中西印刷（株） 2025.7
Language：Japanese Book type：Scholarly book
-
In situ hybridization法
菱川 善隆, 石塚 匠, 柴田 恭明, 小路 武彦（ Role： Joint author）
日本組織細胞化学会・中西印刷（株） 2024.7
Language：Japanese Book type：Scholarly book
-
蛍光共鳴エネルギー移動（FRET）現象を利用した 高感度in situ hybridization
石塚 匠，Narantsog Choijookhuu，柴田 恭明，小路 武彦，菱川 善隆（ Role： Joint author）
日本顕微鏡学会 2023.12
Language：Japanese Book type：Scholarly book
-
In situ hybridization法
菱川 善隆, Narantsog Choijookhuu, 石塚 匠, 柴田 恭明, 小路 武彦（ Role： Joint author）
日本組織細胞化学会・中西印刷（株） 2023.7
Language：Japanese Book type：Scholarly book
-
In situ hybridization法
菱川 善隆, Narantsog Choijookhuu, 石塚 匠, 柴田 恭明, 小路 武彦（ Role： Joint author）
日本組織細胞化学会・中西印刷（株） 2022.7
Language：Japanese Book type：Scholarly book
Presentations 【 display / non-display 】
-
DNA G-quadruplex dynamics during liver regeneration after partial hepatectomy in mice International conference
Takumi Ishizuka, Kham Mo Aung, Toshiki Kubota, Baljinnyam Lkham-Erdene, Kengo Kai, Phyu Synn Oo, Yoshitaka Hishikawa
第52回国際核酸化学シンポジウム/日本核酸化学会第9回年会 (富山国際会議場) 2025.11.12 日本核酸化学会
Event date： 2025.11.12 - 2025.11.14
Language：English Presentation type：Poster presentation
Venue：富山国際会議場
-
蛍光共鳴エネルギー移動（FRET）を応用したモレキュラービーコンプローブによるin situ hybridization Invited
Narantsog Choijookhuu，柴田 恭明，石塚 匠，徐 岩，小路 武彦，菱川 善隆
第66回日本組織細胞化学会総会・学術集会 (大阪公立大学 I-siteなんば) 2025.10.25
Event date： 2025.10.25 - 2025.10.26
Language：Japanese Presentation type：Oral presentation (invited, special)
Venue：大阪公立大学 I-siteなんば
-
超解像顕微鏡による生体分子蛍光イメージング解析 Invited
石塚 匠
第66回日本組織細胞化学会総会・学術集会 (大阪公立大学 I-siteなんば) 2025.10.25
Event date： 2025.10.25 - 2025.10.26
Language：Japanese Presentation type：Symposium, workshop panel (nominated)
Venue：大阪公立大学 I-siteなんば
-
Biological role of DNA G-quadruplex during liver regeneration after partial hepatectomy International conference
Takumi Ishizuka, Kham Mo Aung, Toshiki Kubota, Baljinnyam Lkham-Erdene, Kengo Kai, Yoshitaka Hishikawa
17th international congress of histochemistry and cytochemistry (Rimini, Italy)
Event date： 2025.8.27 - 2025.8.30
Language：English Presentation type：Poster presentation
Venue：Rimini, Italy
-
マウス精子形成過程におけるDNA四重鎖構造の発現動態
石塚 匠, Kham Mo Aung, Baljinnyam Lkham-Erdene, 久保田 壽樹, 甲斐 健吾, 菱川 善隆
第65回日本組織細胞化学会総会・学術集会 (群馬大学医学部) 2024.10.27
Event date： 2024.10.26 - 2024.10.27
Language：Japanese Presentation type：Oral presentation (general)
Venue：群馬大学医学部
Awards 【 display / non-display 】
-
日本組織細胞化学会 Paul K. Nakane AHC Award (Gold medal)
2025.10 日本組織細胞化学会
Narantsog Choijookhuu, Yasuaki Shibata, Takumi Ishizuka, Yan Xu, Takehiko Koji, Yoshitaka Hishikawa
Award type：International academic award (Japan or overseas)
-
日本組織細胞化学会 論文賞
2023.10 日本組織細胞化学会
Narantsog Choijookhuu, Yasuaki Shibata, Takumi Ishizuka, Yan Xu, Takehiko Koji, Yoshitaka Hishikawa
Award type：International academic award (Japan or overseas)
-
FB3 優秀ポスター賞
2012.7 FB3 organizing committee
石塚匠
Award type：Award from international society, conference, symposium, etc. Country：Sweden
-
東京大学大学院工学系研究科長賞（修士）
2008.3 東京大学
石塚匠
Country：Japan
Grant-in-Aid for Scientific Research 【 display / non-display 】
-
精子形成過程から解明するグアニン四重鎖構造の生物学的機能
Grant number：24K08641 2024.04 - 2027.03
独立行政法人日本学術振興会 科学研究費基金 基盤研究(C)
Authorship：Principal investigator
-
PDP効果に基づく胃癌腹膜播種に対する簡便かつ安全な光治療の開発
Grant number：24K11937 2024.04 - 2027.03
独立行政法人日本学術振興会 科学研究費基金 基盤研究(C)
Authorship：Coinvestigator(s)
-
細胞内RNA四重鎖を標的とする構造プローブ法の開発
Grant number：21K05314 2021.04 - 2024.03
独立行政法人日本学術振興会 科学研究費補助金 基盤研究(C)
Authorship：Principal investigator
-
分子結合技術を用いた新たな造影剤による革新的がんMRI画像化技術の開発
Grant number：20K08000 2020.04 - 2023.03
独立行政法人日本学術振興会 科学研究費補助金 基盤研究(C)
Authorship：Coinvestigator(s)
-
分子結合技術を用いた造影剤による革新的がんCT画像化技術の開発
Grant number：19K08156 2019.04 - 2022.03
科学研究費補助金 基盤研究(C)
Authorship：Coinvestigator(s)
Other research activities 【 display / non-display 】
-
第47回組織細胞化学講習会
2022.08
「In situ hybridization法」の技術講習会Wet Labを行った。
Social Activities 【 display / non-display 】
-
解剖見学及び標本示説実習（鵬翔高等学校看護専攻科）
Role(s)： Lecturer
2023.2.1 - 2023.2.2
-
解剖見学及び標本示説実習（九州保健福祉大学総合医療専門学校）
Role(s)： Lecturer
2023.1.31
-
解剖見学及び標本示説実習（宮崎リハビリテーション学院）
Role(s)： Lecturer
2023.1.23
-
解剖見学及び標本示説実習（小林看護医療専門学校）
Role(s)： Lecturer
2023.1.11
-
解剖見学及び標本示説実習（小林看護医療専門学校）
Role(s)： Lecturer
2022.9.2