Affiliation |
Faculty of Medicine School of Medicine Department of Anatomy, Histochemistry and Cell Biology |
Title |
Assistant Professor |
External Link |
ISHIZUKA Takumi
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Research Areas 【 display / non-display 】
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Nanotechnology/Materials / Bio chemistry
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Nanotechnology/Materials / Chemical biology
Education 【 display / non-display 】
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The University of Tokyo Graduate School, Division of Engineering
- 2011.3
Country:Japan
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Tokyo Institute of Technology Faculty of Life Science and Engineering
- 2006.3
Country:Japan
Papers 【 display / non-display 】
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Ikenoue M., Choijookhuu N., Yano K., Fidya , Takahashi N., Ishizuka T., Shirouzu S., Yamaguma Y., Kai K., Higuchi K., Sawaguchi A., Nanashima A., Hishikawa Y.
Histochemistry and Cell Biology 161 ( 4 ) 325 - 336 2024.1
Language:English Publishing type:Research paper (scientific journal) Publisher:Histochemistry and Cell Biology
Su (var) 3–9, enhancer of seste, trithorax (SET)-domain bifurcated histone lysine methyltransferase (SETDB1) plays a crucial role in maintaining intestinal stem cell homeostasis; however, its physiological function in epithelial injury is largely unknown. In this study, we investigated the role of SETDB1 in epithelial regeneration using an intestinal ischemia/reperfusion injury (IRI) mouse model. Jejunum tissues were sampled after 75 min of ischemia followed by 3, 24, and 48 h of reperfusion. Morphological evaluations were performed using light microscopy and electron microscopy, and the involvement of SETDB1 in epithelial remodeling was investigated by immunohistochemistry. Expression of SETDB1 was increased following 24 h of reperfusion and localized in not only the crypt bottom but also in the transit amplifying zone and part of the villi. Changes in cell lineage, repression of cell adhesion molecule expression, and decreased histone H3 methylation status were detected in the crypts at the same time. Electron microscopy also revealed aberrant alignment of crypt nuclei and fusion of adjacent villi. Furthermore, increased SETDB1 expression and epithelial remodeling were confirmed with loss of stem cells, suggesting SETDB1 affects epithelial cell plasticity. In addition, crypt elongation and increased numbers of Ki-67 positive cells indicated active cell proliferation after IRI; however, the expression of PCNA was decreased compared to sham mouse jejunum. These morphological changes and the aberrant expression of proliferation markers were prevented by sinefungin, a histone methyltransferase inhibitor. In summary, SETDB1 plays a crucial role in changes in the epithelial structure after IRI-induced stem cell loss.
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Protective role of estrogen through G-protein coupled receptor 30 in a colitis mouse model Reviewed
Fidya, Narantsog Choijookhuu, Makoto Ikenoue, Koichi Yano, Yu Yamaguma, Shinichiro Shirouzu, Kengo Kai, Takumi Ishizuka, Yoshitaka Hishikawa
Histochemistry and Cell Biology 161 ( 1 ) 81 - 93 2023.10
Language:English Publishing type:Research paper (scientific journal)
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Pivotal role of High-Mobility Group Box 2 in ovarian folliculogenesis and fertility Reviewed
Shinichiro Shirouzu, Naohiro Sugita, Narantsog Choijookhuu, Yu Yamaguma, Kanako Takeguchi, Takumi Ishizuka, Mio Tanaka, Fidya, Kengo Kai, Etsuo Chosa, Yoshihiro Yamashita, Chihiro Koshimoto, Yoshitaka Hishikawa
Journal of Ovarian Research 15 ( 1 ) 133 - 133 2022.12
Language:English Publishing type:Research paper (scientific journal)
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Narantsog Choijookhuu, Yasuaki Shibata, Takumi Ishizuka, Yan Xu, Takehiko Koji, Yoshitaka Hishikawa
Acta Histochemica et Cytochemica 55 ( 5 ) 119 - 128 2022.10
Language:English Publishing type:Research paper (scientific journal)
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Zhiyong He, Takumi Ishizuka, Yoshitaka Hishikawa, Yan Xu
Chemical Communications 58 12479- - 12482 2022.10
Language:English Publishing type:Research paper (scientific journal)
Books 【 display / non-display 】
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蛍光共鳴エネルギー移動(FRET)現象を利用した 高感度in situ hybridization
石塚 匠,Narantsog Choijookhuu,柴田 恭明,小路 武彦,菱川 善隆( Role: Joint author)
日本顕微鏡学会 2023.12
Language:Japanese Book type:Scholarly book
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In situ hybridization法
菱川 善隆, Narantsog Choijookhuu, 石塚 匠, 柴田 恭明, 小路 武彦( Role: Joint author)
日本組織細胞化学会・中西印刷(株) 2022.7
Language:Japanese Book type:Scholarly book
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病理と臨床 2022年臨時増刊号(40巻): がんゲノム医療時代の分子腫瘍学
菱川 善隆, Narantsog Choijookhuu, 石塚 匠, 柴田 恭明, 小路 武彦( Role: Joint author , 第1部 がんの分子病理学(序論):FISH・ISH)
文光堂 2022.4
Language:Japanese Book type:Scholarly book
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G-Quadruplex Nucleic Acids
Takumi Ishizuka, Hong-Liang Bao, Yan Xu( Role: Joint author , Chapter 26: 19F NMR Spectroscopy for the Analysis of DNA G-quadruplex Structures using 19F-labeled Nucleobase)
Springer 2019.8
Language:English Book type:Scholarly book
Presentations 【 display / non-display 】
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マウス肝再生過程におけるDNA四重鎖構造の発現動態
石塚 匠, Narantsog Choijookhuu, 甲斐 健吾, 菱川 善隆
⽇本ケミカルバイオロジー学会 第18回年会 (九州工業大学戸畑キャンパス) 2024.5.28
Event date: 2024.5.27 - 2024.5.29
Language:Japanese Presentation type:Poster presentation
Venue:九州工業大学戸畑キャンパス
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肝再生過程におけるDNA四重鎖構造の肝細胞周期への関与
石塚 匠, Narantsog Choijookhuu, 白水 慎一郎, Fidya, 甲斐 健吾, 菱川 善隆
第129回 日本解剖学会総会・全国学術集会 (九州工業大学戸畑キャンパス) 2024.3.22
Event date: 2024.3.21 - 2024.3.23
Language:Japanese Presentation type:Oral presentation (general)
Venue:九州工業大学戸畑キャンパス
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マウス肝再生過程におけるDNA四重鎖構造の肝細胞周期への関与
石塚 匠, Narantsog Choijookhuu, 白水 慎一郎, Fidya, 甲斐 健吾, 菱川 善隆
第65回日本顕微鏡学会九州支部集会 (九州工業大学戸畑キャンパス) 2023.12.9
Event date: 2023.12.9
Language:Japanese Presentation type:Oral presentation (general)
Venue:九州工業大学戸畑キャンパス
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肝再生過程におけるグアニン四重鎖構造の肝細胞増殖への関与
石塚 匠, Narantsog Choijookhuu, 白水 慎一郎, Fidya, 甲斐 健吾, 菱川 善隆
日本解剖学会 第79回 九州支部学術集会 (産業医科大学) 2023.11.11
Event date: 2023.11.11
Language:Japanese Presentation type:Oral presentation (general)
Venue:産業医科大学
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マウス肝再生過程におけるグアニン四重鎖構造の発現動態研究
石塚 匠, Narantsog Choijookhuu, 白水 慎一郎, Fidya, 甲斐 健吾, 菱川 善隆
第64回日本組織細胞化学会総会・学術集会 (エッサム神田ホール) 2023.10.20
Event date: 2023.10.20 - 2023.10.21
Language:Japanese Presentation type:Oral presentation (general)
Venue:エッサム神田ホール
Awards 【 display / non-display 】
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2023年度 日本組織細胞化学会 論文賞
2023.10 日本組織細胞化学会
Narantsog Choijookhuu, Yasuaki Shibata, Takumi Ishizuka, Yan Xu, Takehiko Koji, Yoshitaka Hishikawa
Award type:International academic award (Japan or overseas)
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FB3 優秀ポスター賞
2012.7 FB3 organizing committee
石塚匠
Award type:Award from international society, conference, symposium, etc. Country:Sweden
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東京大学大学院工学系研究科長賞(修士)
2008.3 東京大学
石塚匠
Country:Japan
Grant-in-Aid for Scientific Research 【 display / non-display 】
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細胞内RNA四重鎖を標的とする構造プローブ法の開発
Grant number:21K05314 2021.04 - 2024.03
独立行政法人日本学術振興会 科学研究費補助金 基盤研究(C)
Authorship:Principal investigator
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分子結合技術を用いた新たな造影剤による革新的がんMRI画像化技術の開発
Grant number:20K08000 2020.04 - 2023.03
独立行政法人日本学術振興会 科学研究費補助金 基盤研究(C)
Authorship:Coinvestigator(s)
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分子結合技術を用いた造影剤による革新的がんCT画像化技術の開発
Grant number:19K08156 2019.04 - 2022.03
科学研究費補助金 基盤研究(C)
Authorship:Coinvestigator(s)
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高感度蛍光in situハイブリダイゼーション法によるmicroRNA発現解析
Grant number:16K08471 2016.04 - 2019.03
科学研究費補助金 基盤研究(C)
Authorship:Coinvestigator(s)
FRETを利用して、組織切片上で特定の細胞での小分子非翻訳RNAであるmicroRNA発現動態を解析する新たな検出法を開発する。
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特殊な四重鎖構造の解析を可能とする新規化学修飾法の開発とその応用
Grant number:16K17938 2016.04 - 2018.03
科学研究費補助金 若手研究(B)
Authorship:Principal investigator
Other research activities 【 display / non-display 】
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第47回組織細胞化学講習会
2022.08
「In situ hybridization法」の技術講習会Wet Labを行った。
Social Activities 【 display / non-display 】
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解剖見学及び標本示説実習(鵬翔高等学校看護専攻科)
Role(s): Lecturer
2023.2.1 - 2023.2.2
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解剖見学及び標本示説実習(九州保健福祉大学総合医療専門学校)
Role(s): Lecturer
2023.1.31
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解剖見学及び標本示説実習(宮崎リハビリテーション学院)
Role(s): Lecturer
2023.1.23
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解剖見学及び標本示説実習(小林看護医療専門学校)
Role(s): Lecturer
2023.1.11
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解剖見学及び標本示説実習(小林看護医療専門学校)
Role(s): Lecturer
2022.9.2