研究者詳細 - 石塚　匠

論文 - 石塚　匠

分割表示 44 件中 1 - 20 件目／全件表示 >>

SETDB1-Mediated Chromatin Regulation in Intestinal Epithelial Cells During Intestinal Ischemia-Reperfusion Injury 査読あり

Higuchi K, Ikenoue M, Ishizuka T, Kai K, Takahashi N, Kubota T, Shirouzu S, Lkham-Erdene B, Mo Aung K, Nakai M, Sawaguchi A, Nanashima A, Hishikawa Y

Acta Histochemica et Cytochemica 58 ( 1 ) 9 - 18 2025年2月

記述言語：英語掲載種別：研究論文（学術雑誌）

DOI： 10.1267/ahc.24-00061

Effect of Hepatic Lipid Overload on Accelerated Hepatocyte Proliferation Promoted by HGF Expression via the SphK1/S1PR2 Pathway in MCD-diet Mouse Partial Hepatectomy 査読あり

Lkham-Erdene B., Choijookhuu N., Kubota T., Uto T., Mitoma S., Shirouzu S., Ishizuka T., Kai K., Higuchi K., Aung K.M., Batmunkh J.E., Sato K., Hishikawa Y.

Acta Histochemica et Cytochemica 57 ( 5 ) 175 - 188 2024年10月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Acta Histochemica et Cytochemica

Metabolic dysfunction-associated steatotic liver disease (MASLD) is becoming a major health problem worldwide. Liver regeneration is crucial for restoring liver function, and is regulated by extraordinary complex process, involving numerous factors under both physiologic and pathologic conditions. Sphingosine-1-phosphate (S1P), a bioactive sphingolipid synthesized by sphingosine kinase 1 (SphK1), plays an important role in liver function through S1P receptors (S1PRs)-expressing cells. In this study, we investigated the effect of lipid overload on hepatocyte proliferation in a mouse hepatic steatosis model induced by feeding a methionine-and choline-deficient (MCD) diet. After 50% partial hepatectomy (PHx), liver tissues were sampled at various timepoints and then analyzed by immunohistochemistry, oil Red-O staining, quantitative-polymerase chain reaction (qPCR), and flow cytometry. In mice fed the MCD-diet, significantly exacerbated hepatic steatosis and accelerated liver regeneration were observed. After PHx, hepatocyte proliferation peaked at 48 and 36 hr in the liver of chow-and MCD-diet fed mice, respectively. By contrast, increased expression of S1PR2 was observed in hepatic neutrophils and macrophages of MCD-diet fed mice. Flow cytome-try and qPCR experiments demonstrated that levels of HGF and FGF2 released by neutrophils and macrophages were significantly higher in MCD-diet fed mice. In conclusion, hepatic lipid overload recruits Kupffer cells and neutrophils that release HGF and FGF2 via SphK1/S1PR2 activation to accelerate hepatocyte proliferation.

DOI： 10.1267/ahc.24-00046

Scopus

CiNii Research

The crucial role of SETDB1 in structural and functional transformation of epithelial cells during regeneration after intestinal ischemia reperfusion injury 査読あり

Ikenoue M., Choijookhuu N., Yano K., Fidya , Takahashi N., Ishizuka T., Shirouzu S., Yamaguma Y., Kai K., Higuchi K., Sawaguchi A., Nanashima A., Hishikawa Y.

Histochemistry and Cell Biology 161 ( 4 ) 325 - 336 2024年4月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Histochemistry and Cell Biology

Su (var) 3–9, enhancer of seste, trithorax (SET)-domain bifurcated histone lysine methyltransferase (SETDB1) plays a crucial role in maintaining intestinal stem cell homeostasis; however, its physiological function in epithelial injury is largely unknown. In this study, we investigated the role of SETDB1 in epithelial regeneration using an intestinal ischemia/reperfusion injury (IRI) mouse model. Jejunum tissues were sampled after 75 min of ischemia followed by 3, 24, and 48 h of reperfusion. Morphological evaluations were performed using light microscopy and electron microscopy, and the involvement of SETDB1 in epithelial remodeling was investigated by immunohistochemistry. Expression of SETDB1 was increased following 24 h of reperfusion and localized in not only the crypt bottom but also in the transit amplifying zone and part of the villi. Changes in cell lineage, repression of cell adhesion molecule expression, and decreased histone H3 methylation status were detected in the crypts at the same time. Electron microscopy also revealed aberrant alignment of crypt nuclei and fusion of adjacent villi. Furthermore, increased SETDB1 expression and epithelial remodeling were confirmed with loss of stem cells, suggesting SETDB1 affects epithelial cell plasticity. In addition, crypt elongation and increased numbers of Ki-67 positive cells indicated active cell proliferation after IRI; however, the expression of PCNA was decreased compared to sham mouse jejunum. These morphological changes and the aberrant expression of proliferation markers were prevented by sinefungin, a histone methyltransferase inhibitor. In summary, SETDB1 plays a crucial role in changes in the epithelial structure after IRI-induced stem cell loss.

DOI： 10.1007/s00418-023-02263-9

Scopus

HMGB2 Promotes De Novo Lipogenesis to Accelerate Hepatocyte Proliferation During Liver Regeneration 査読あり

Choijookhuu N., Yano K., Lkham-Erdene B., Shirouzu S., Kubota T., Fidya , Ishizuka T., Kai K., Chosa E., Hishikawa Y.

Journal of Histochemistry and Cytochemistry 72 ( 4 ) 245 - 264 2024年4月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Journal of Histochemistry and Cytochemistry

Liver regeneration is a well-orchestrated compensatory process that is regulated by multiple factors. We recently reported the importance of the chromatin protein, a high-mobility group box 2 (HMGB2) in mouse liver regeneration. However, the molecular mechanism remains unclear. In this study, we aimed to study how HMGB2 regulates hepatocyte proliferation during liver regeneration. Seventy-percent partial hepatectomy (PHx) was performed in wild-type (WT) and HMGB2-knockout (KO) mice, and the liver tissues were used for microarray, immunohistochemistry, quantitative polymerase chain reaction (qPCR), and Western blotting analyses. In the WT mice, HMGB2-positive hepatocytes colocalized with cell proliferation markers. In the HMGB2-KO mice, hepatocyte proliferation was significantly decreased. Oil Red O staining revealed the transient accumulation of lipid droplets at 12–24 hr after PHx in the WT mouse livers. In contrast, decreased amount of lipid droplets were found in HMGB2-KO mouse livers, and it was preserved until 36 hr. The microarray, immunohistochemistry, and qPCR results demonstrated that the expression of lipid metabolism–related genes was significantly decreased in the HMGB2-KO mouse livers. The in vitro experiments demonstrated that a decrease in the amount of lipid droplets correlated with decreased cell proliferation activity in HMGB2-knockdown cells. HMGB2 promotes de novo lipogenesis to accelerate hepatocyte proliferation during liver regeneration:

DOI： 10.1369/00221554241241569

Scopus

CiNii Research

Protective role of estrogen through G-protein coupled receptor 30 in a colitis mouse model 査読あり

Fidya, Narantsog Choijookhuu, Makoto Ikenoue, Koichi Yano, Yu Yamaguma, Shinichiro Shirouzu, Kengo Kai, Takumi Ishizuka, Yoshitaka Hishikawa

Histochemistry and Cell Biology 161 ( 1 ) 81 - 93 2023年10月

記述言語：英語掲載種別：研究論文（学術雑誌）

DOI： 10.1186/s13048-022-01071-4

Pivotal role of High-Mobility Group Box 2 in ovarian folliculogenesis and fertility 査読あり

Shinichiro Shirouzu, Naohiro Sugita, Narantsog Choijookhuu, Yu Yamaguma, Kanako Takeguchi, Takumi Ishizuka, Mio Tanaka, Fidya, Kengo Kai, Etsuo Chosa, Yoshihiro Yamashita, Chihiro Koshimoto, Yoshitaka Hishikawa

Journal of Ovarian Research 15 ( 1 ) 133 - 133 2022年12月

記述言語：英語掲載種別：研究論文（学術雑誌）

DOI： 10.1186/s13048-022-01071-4

An Advanced Detection System for In Situ Hybridization Using a Fluorescence Resonance Energy Transfer-based Molecular Beacon Probe 査読あり

Narantsog Choijookhuu, Yasuaki Shibata, Takumi Ishizuka, Yan Xu, Takehiko Koji, Yoshitaka Hishikawa

Acta Histochemica et Cytochemica 55 ( 5 ) 119 - 128 2022年10月

記述言語：英語掲載種別：研究論文（学術雑誌）

DOI： https://doi.org/10.1267/ahc.22-00075

Click chemistry for fluorescence imaging via combination of a BODIPY-based ‘turn-on’ probe and a norbornene glucosamine 査読あり

Zhiyong He, Takumi Ishizuka, Yoshitaka Hishikawa, Yan Xu

Chemical Communications 58 12479- - 12482 2022年10月

記述言語：英語掲載種別：研究論文（学術雑誌）

DOI： https://doi.org/10.1039/D2CC05359D

Crucial role of high-mobility group box 2 in mouse ovarian follicular development through estrogen receptor beta 査読あり

Yu Yamaguma, Naohiro Sugita, Narantsog Choijookhuu, Koichi Yano, Deokcheol Lee, Makoto Ikenoue, Fidya, Shinichiro Shirouzu, Takumi Ishizuka, Mio Tanaka, Yoshihiro Yamashita, Etsuo Chosa, Noboru Taniguchi, Yoshitaka Hishikawa

Histochemistry and Cell Biology 157 ( 3 ) 359 - 369 2022年1月

記述言語：英語掲載種別：研究論文（学術雑誌）

DOI： https://doi.org/10.1007/s00418-022-02074-4

Systematic Approach to DNA Aptamer Design Using Amino Acid–Nucleic Acid Hybrids (ANHs) Targeting Thrombin 査読あり

Ji Hye Yum, Takumi Ishizuka, Koyuki Fukumoto, Daisuke Hori, Hong-Liang Bao, Yan Xu, Hiroshi Sugiyama, Soyoung Park

ACS Biomaterials Science and Engineering 7 ( 4 ) 1338 - 1343 2021年3月

記述言語：英語掲載種別：研究論文（学術雑誌）

DOI： 10.1021/acsbiomaterials.1c00060

Improving Thermodynamic Stability and Anticoagulant Activity of a Thrombin Binding Aptamer by Incorporation of 8-trifluoromethyl-2'-deoxyguanosine 査読あり

Hong-Liang Bao, Takumi Ishizuka, Atsushi Yamashita, Eiji Furukoji, Yujiro Asada, Yan Xu

Journal of Medicinal Chemistry 64 ( 1 ) 711 - 718 2020年12月

記述言語：英語掲載種別：研究論文（学術雑誌）

DOI： 10.1021/acs.jmedchem.0c01711

Linear consecutive hexaoxazoles as G4 ligands inducing chair-type anti-parallel topology of a telomeric G-quadruplex 査読あり

Shogo Sasaki, Yue Ma, Takumi Ishizuka, Hong-Liang Bao, Takatsugu Hirokawa, Yan Xu, Masayuki Tera, Kazuo Nagasawa

RSC Advances 10 ( 71 ) 43319 - 43323 2020年12月

記述言語：英語掲載種別：研究論文（学術雑誌）

DOI： 10.1039/D0RA09413G

Influenza Virus Z-RNAs Induce ZBP1-Mediated Necroptosis 査読あり

Ting Zhang, Chaoran Yin, David F. Boyd, Giovanni Quarato, Justin P. Ingram, Maria Shubina, Katherine B. Ragan, Takumi Ishizuka, Jeremy Chase Crawford, Bart Tummers, Diego A. Rodriguez, Jia Xue, Suraj Peri, William J. Kaiser, Carolina B. López, Yan Xu, Jason W. Upton, Paul G. Thomas, Douglas R. Green, Siddharth Balachandran

Cell 180 ( 6 ) 1115 - 1129 2020年3月

記述言語：英語掲載種別：研究論文（学術雑誌）

DOI： 10.1016/j.cell.2020.02.050

Characterization of Structure and Catalytic Activity of a Complex between Heme and an All Parallel-Stranded Tetrameric G-Quadruplex Formed from DNA/RNA Chimera Sequence d(TTA)r(GGG)dT 査読あり

Kosuke Hayasaka, Tomokazu Shibata, Aya Sugahara, Atsuya Momotake, Toru Matsui, Saburo Neya, Takumi Ishizuka, Yan Xu, Yasuhiko Yamamoto

Bulletin of the Chemical Society of Japan 93 ( 5 ) 621 - 629 2020年2月

記述言語：英語掲載種別：研究論文（学術雑誌）

DOI： 10.1246/bcsj.20200013

A Nucleoside Derivative 5-Vinyluridine (VrU) for Imaging RNA in Cells and Animals 査読あり

Hong-Shan Liu, Takumi Ishizuka, Makiko Kawaguchi, Ryuichi Nishii, Hiroaki Kataoka, Yan Xu

Bioconjugate Chemistry 30 ( 11 ) 2958 - 2966 2019年10月

記述言語：英語掲載種別：研究論文（学術雑誌）