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Affiliation |
Faculty of Agriculture Department of Marine Biological and Environmental Science |
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NAGANO Naoki
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Research Areas 【 display / non-display 】
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Life Science / Aquatic bioproduction science
Papers 【 display / non-display 】
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Ohga H., Shibata K., Sakanoue R., Ogawa T., Kitano H., Kai S., Ohta K., Nagano N., Nagasako T., Uchida S., Sakuma T., Yamamoto T., Kim S., Tashiro K., Kuhara S., Gen K., Fujiwara A., Kazeto Y., Kobayashi T., Matsuyama M.
Scientific Reports 13 ( 1 ) 3190 2023.2
Language：English Publishing type：Research paper (scientific journal) Publisher：Scientific Reports
Genome editing is a technology that can remarkably accelerate crop and animal breeding via artificial induction of desired traits with high accuracy. This study aimed to develop a chub mackerel variety with reduced aggression using an experimental system that enables efficient egg collection and genome editing. Sexual maturation and control of spawning season and time were technologically facilitated by controlling the photoperiod and water temperature of the rearing tank. In addition, appropriate low-temperature treatment conditions for delaying cleavage, shape of the glass capillary, and injection site were examined in detail in order to develop an efficient and robust microinjection system for the study. An arginine vasotocin receptor V1a2 (V1a2) knockout (KO) strain of chub mackerel was developed in order to reduce the frequency of cannibalistic behavior at the fry stage. Video data analysis using bioimage informatics quantified the frequency of aggressive behavior, indicating a significant 46% reduction (P = 0.0229) in the frequency of cannibalistic behavior than in wild type. Furthermore, in the V1a2 KO strain, the frequency of collisions with the wall and oxygen consumption also decreased. Overall, the manageable and calm phenotype reported here can potentially contribute to the development of a stable and sustainable marine product.
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Reproductive farming technology in Japanese eel and chub mackerel Reviewed
Miyanishi H., Nagano N.
Aquaculture and Fisheries 7 ( 5 ) 595 - 600 2022.9
Authorship：Last author Language：English Publishing type：Research paper (scientific journal) Publisher：Aquaculture and Fisheries
Japanese eel (Anguilla japonica) and chub mackerel (Scomber japonicus) are commercially valuable species in Asian aquaculture. The reduction in eel and chub mackerel resources has been a serious problem in recent years that should be addressed by reducing the catch of their natural populations to halt their decline and reach the Sustainable Development Goals. Furthermore, securing sufficient food supply by aquaculture is an important step in addressing the increasing demand for fish products in recent years. Traditionally, juveniles of the Japanese eel and chub mackerel have been captured to be raised in aquaculture. Owing to the extensive research on these species, new technologies have been developed for full-lifecycle aquaculture of Japanese eel in 2011 and chub mackerel in 2014. These technologies are expected to stop the decline of natural resources and provide a stable food supply. Recently, seed production of these species has increased owing to the development of successful broodstock management and larval rearing techniques. Fundamental information on oocyte maturation and ovulation and its application for artificial induction of sexual maturation is needed to produce good quality seeds of the Japanese eel and chub mackerel. Here, hormonal mechanisms and previously and newly developed methods for artificial seed production have been described.
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Diel vertical migration of chub mackerel: preliminary evidence from a biologging study Reviewed
Yasuda T., Nagano N., Kitano H.
Marine Ecology Progress Series 598 147 - 151 2018.6
Language：English Publishing type：Research paper (scientific journal) Publisher：Marine Ecology Progress Series
© Inter-Research 2018. Fishes in the genus Scomber are migratory species specialised for rapid and efficient swimming, and are also of high commercial value. Despite advances in understanding the horizontal distribution and migratory behaviour of Scomber species, knowledge remains limited about their vertical movement patterns. This paper presents a proof of concept showing the potential for using biologging techniques to help understand the vertical movement patterns of a scombrid. The vertical swimming behaviour of 1 chub mackerel S. japonicus was measured at 10 s intervals for 166 consecutive days from November to April with an electronic tag. The tagged fish showed a normal pattern of diel vertical migration (DVM) that involved movement from shallow depths at night to greater depths during the day. However, this pattern of DVM broke down or reversed on a short-term basis during the overall recording period. In addition, the fish modified the depth and amplitude of DVM in response to the vertical gradient of the water temperature. Consequently, even though the tagged individual used a wide range of depths uniformly (0 to 130 m), it remained within potentially physiologically adequate ambient water temperatures in a vertically and seasonally heterogeneous thermal environment.
DOI： 10.3354/meps12636
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Kitano H., Nagano N., Sakaguchi K., Matsuyama M.
Aquaculture Research 49 ( 1 ) 352 - 358 2018.1
Language：English Publishing type：Research paper (scientific journal) Publisher：Aquaculture Research
© 2017 John Wiley & Sons Ltd Chub mackerel (Scomber japonicus Houttuyn) is one of the most commercially important scombroid fish used as a food resource. Recently, there has been a demand for efficient rearing methods of this fish for a full-life cycle aquaculture. In the present study, we evaluated the physiological responses in the juvenile S. japonicus to different ambient salinities. A significantly higher gain of the body mass was observed in the juveniles reared in 24 g/L and 13 g/L seawater than in those reared in natural seawater (34 g/L) within 40 days of the experimental period without affecting mortality. A principal enzyme for osmoregulation, Na + /K + -ATPase, was expressed in the ionocytes located in the gill filaments of the juveniles. The number and the cell size of ionocytes and the enzymatic activity of Na + /K + -ATPase in the gills decreased within 10 days after the low-salinity challenge, which implies the reduction of the energy-consuming active ion secretion under the low-salinity environment. The physiological capacity for adaptation to low-salinity seawater in chub mackerel could be basic knowledge to carry out culturing of these fish in coastal sea pens where ambient salinity fluctuates. The improvement of the growth performance by rearing in low-salinity seawater will contribute to the efficient production of the seed juveniles for aquaculture.
DOI： 10.1111/are.13465
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4. Regional activation with full-life cycle aquaculture of chub mackerel Invited
NAGANO NAOKI
NIPPON SUISAN GAKKAISHI 83 ( 5 ) 839 - 839 2017.9
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：The Japanese Society of Fisheries Science
MISC 【 display / non-display 】
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マサバの人工種苗生産の現状 Invited
松山 倫也、長野 直樹
月刊養殖ビジネス 61 ( 4 ) 116 - 119 2024.3
Authorship：Corresponding author Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media) Publisher：緑書房
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マサバ養殖の急増から見る市場規模拡大と生産・流通の特徴 Invited
長野 直樹
月刊養殖ビジネス 56 ( 10 ) 4 - 7 2019.9
Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media) Publisher：緑書房
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北浦養殖マサバ協業体の「ひむか本サバ」(宮崎県) Invited
長野 直樹
月刊養殖ビジネス 56 ( 10 ) 38 - 39 2019.9
Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media) Publisher：緑書房
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佐賀玄海漁協の「唐津Qサバ」(佐賀県) Invited
長野 直樹
月刊養殖ビジネス 56 ( 10 ) 32 - 33 2019.9
Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media) Publisher：緑書房
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マサバ養殖における人工種苗の強みと課題． Invited
長野 直樹
月刊養殖ビジネス 56 ( 1 ) 36 - 38 2019.1
Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media)
Presentations 【 display / non-display 】
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ゲノム編集と組換え濾胞刺激ホルモンによるマサバの生殖統御の試み
佐藤桜雅・Tapas Chakraborty・加藤洋教・ 八尋逸清・入路光雄・伊藤武彦・ 長野直樹・松山倫也・太田耕平
令和6年度日本水産学会春季大会 2024.3.29
Event date： 2024.3.27 - 2024.3.30
Language：Japanese Presentation type：Oral presentation (general)
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Characterization of temperature-sensitive Transient Receptor Potential Cation Channel Subfamily Vanilloid (TRPV) 1 and its modulation of fshb expression in chub mackerel (Scomber japonicus)
Kyle Dominic Barnuevo・Hironori Katoh・Sipra Mohapatra ・ Naoki Nagano・ Takehiko Itoh・ Michiya Matsuyama・ Tapas Chakraborty・Kohei Ohta
令和6年度日本水産学会春季大会 2024.3.30
Event date： 2024.3.27 - 2024.3.30
Language：English Presentation type：Oral presentation (general)
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ゲノム編集により作出したおとなしいマサバのオフターゲット解析
竹内 諒・大野 薫・大賀浩史・ 伊藤武彦・長野直樹・藤原篤志・佐久間哲史・ 山本 卓・Tapas Chakraborty・太田耕平・松山倫也
令和6年度日本水産学会春季大会 2024.3.30
Event date： 2024.3.27 - 2024.3.30
Language：Japanese Presentation type：Oral presentation (general)
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ゲノム編集と組換え濾胞刺激ホルモンによるマサバの⽣殖統御の試み
佐藤桜雅・八尋逸清・Chakraborty Tapas・入路光雄・伊藤武彦・長野直樹・松山倫也・太田耕平
令和５年度 ⽇本⽔産増殖学会第21回⼤会 2023.12.2
Event date： 2023.12.2 - 2023.12.3
Language：Japanese Presentation type：Oral presentation (general)
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ニホンウナギのビリ解決に資する新規成長促進法の確立
宮西弘 ・ 鎌田優功・後藤暁彦・黒木真理・長野直樹
令和5年度日本水産学会秋季大会 2023.9.20
Event date： 2023.9.19 - 2023.9.22
Language：Japanese Presentation type：Oral presentation (general)
Grant-in-Aid for Scientific Research 【 display / non-display 】
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メタボローム解析による養殖魚の卵質評価に有効なバイオマーカーの探索
Grant number：19K06190 2019.04 - 2023.03
科学研究費補助金 基盤研究(C)
Authorship：Principal investigator
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完全養殖系とゲノム編集技術を用いた海産魚における新規育種基盤技術の開発
Grant number：15H02459 2015.04 - 2019.03
科学研究費補助金 基盤研究(A)
Authorship：Coinvestigator(s)
Available Technology 【 display / non-display 】
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海産魚の完全養殖技術開発
牡蠣類養殖に関する基礎的研究
水産物の機能性成分に関する定量測定技術の開発・実用化Related fields where technical consultation is available：魚介類の種苗生産および養殖技術開発
天然魚および養殖魚の一般成分・脂肪酸（EPA・DHA）の分析
高品質・低コスト飼料の開発