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農学部 海洋生物環境学科 |
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Diel vertical migration of chub mackerel: preliminary evidence from a biologging study 査読あり
Yasuda T., Nagano N., Kitano H.
Marine Ecology Progress Series 598 147 - 151 2018年6月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Marine Ecology Progress Series
© Inter-Research 2018. Fishes in the genus Scomber are migratory species specialised for rapid and efficient swimming, and are also of high commercial value. Despite advances in understanding the horizontal distribution and migratory behaviour of Scomber species, knowledge remains limited about their vertical movement patterns. This paper presents a proof of concept showing the potential for using biologging techniques to help understand the vertical movement patterns of a scombrid. The vertical swimming behaviour of 1 chub mackerel S. japonicus was measured at 10 s intervals for 166 consecutive days from November to April with an electronic tag. The tagged fish showed a normal pattern of diel vertical migration (DVM) that involved movement from shallow depths at night to greater depths during the day. However, this pattern of DVM broke down or reversed on a short-term basis during the overall recording period. In addition, the fish modified the depth and amplitude of DVM in response to the vertical gradient of the water temperature. Consequently, even though the tagged individual used a wide range of depths uniformly (0 to 130 m), it remained within potentially physiologically adequate ambient water temperatures in a vertically and seasonally heterogeneous thermal environment.
DOI： 10.3354/meps12636
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Kitano H., Nagano N., Sakaguchi K., Matsuyama M.
Aquaculture Research 49 ( 1 ) 352 - 358 2018年1月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Aquaculture Research
© 2017 John Wiley & Sons Ltd Chub mackerel (Scomber japonicus Houttuyn) is one of the most commercially important scombroid fish used as a food resource. Recently, there has been a demand for efficient rearing methods of this fish for a full-life cycle aquaculture. In the present study, we evaluated the physiological responses in the juvenile S. japonicus to different ambient salinities. A significantly higher gain of the body mass was observed in the juveniles reared in 24 g/L and 13 g/L seawater than in those reared in natural seawater (34 g/L) within 40 days of the experimental period without affecting mortality. A principal enzyme for osmoregulation, Na + /K + -ATPase, was expressed in the ionocytes located in the gill filaments of the juveniles. The number and the cell size of ionocytes and the enzymatic activity of Na + /K + -ATPase in the gills decreased within 10 days after the low-salinity challenge, which implies the reduction of the energy-consuming active ion secretion under the low-salinity environment. The physiological capacity for adaptation to low-salinity seawater in chub mackerel could be basic knowledge to carry out culturing of these fish in coastal sea pens where ambient salinity fluctuates. The improvement of the growth performance by rearing in low-salinity seawater will contribute to the efficient production of the seed juveniles for aquaculture.
DOI： 10.1111/are.13465
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Kitano H., Nagano N., Sakaguchi K., Matsuyama M.
Molecular Reproduction and Development 84 ( 5 ) 363 - 375 2017年5月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Molecular Reproduction and Development
© 2017 Wiley Periodicals, Inc. Vitellogenenesis is a physiological process common in oviparous animals. The molecular profile, modifications, and utilization of vitellogenin (VTG), a precursor of yolk protein, have been characterized in various taxa to understand oogenesis within different modes of reproduction. Hormonal regulation of VTGs has been investigated in invertebrates, such as insects and crustaceans; conversely, little is known for cephalopods. In this study, we isolated two VTG genes (ue-VTG1 and ue-VTG2) from the loliginid swordtip squid, Uroteuthis edulis, via a comprehensive survey of a transcriptome database and subsequent cDNA cloning. Structural analysis of the two ue-VTGs revealed their unique features, namely the absence of two domains usually found in VTGs from other organisms: the von Willebrand factor D domain (vWD) and the domain of unknown function 1943 (DUF1943). Levels of ue-VTG1 and ue-VTG2 transcripts in the ovary, specifically in follicular cells, increased during the late-vitellogenic phase, suggesting that yolk accumulation progresses via paracrine interactions involving follicular cells and oocytes. N-terminal amino acid sequencing of biochemically purified yolk protein revealed its origins from these two VTGs, indicating that both are functional precursors of yolk protein. These results provide information that is essential to understanding the physiological pathway of yolk synthesis, accumulation, and storage in loliginid squids.
DOI： 10.1002/mrd.22786
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Ohga H., Hirata D., Matsumori K., Kitano H., Nagano N., Yamaguchi A., Matsuyama M.
Comparative Biochemistry and Physiology -Part A : Molecular and Integrative Physiology 203 159 - 166 2017年1月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Comparative Biochemistry and Physiology -Part A : Molecular and Integrative Physiology
© 2016 Elsevier Inc. Leptin directly regulates kisspeptin neurons in the hypothalamus and gonadotropin secretion from the pituitary, making it a central player in the onset of mammalian puberty. Recently, we identified two leptin genes (lepa and lepb) and a single leptin receptor (lepr) in the marine perciform fish chub mackerel; however, the expression of these genes did not correlate with the expression of important reproductive genes or ovarian stage during female puberty. Here, we expand upon these initial observations by evaluating the expression of lepa, lepb, and lepr during pubertal transition and under differential feeding conditions in the male chub mackerel. Reverse transcription-polymerase chain reaction (RT-PCR) showed that lepa was primarily expressed in the liver of pubertal and gonadal recrudescence adults, as well as in the brain of adult fish; lepb was primarily expressed in the brain of all fish tested; and lepr was widely expressed in a variety of tissues. qRT-PCR analyses revealed significant increases in the hepatic expression of lepa in accordance with testicular stage, whereas pituitary follicle-stimulating hormone (fshβ) expression increased in unison with hepatic lepa. In contrast, expression of both brain lepa and lepb dramatically decreased during pubertal transition, with brain kisspeptin 1 (kiss1) expression strongly correlating with leptin expression patterns. In pre-pubertal males, lepa, lepb, and lper gene expression in the brain, pituitary gland, and liver decreased in fish given a high feed diet, relative to the controlled feeding group. Taken together, these results indicate high sexual specificity of leptin expression, suggesting a possible role for leptin signaling in endocrine and neuroendocrine functions during spermatogenesis in the pubertal male chub mackerel.
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マサバ養殖の急増から見る市場規模拡大と生産・流通の特徴 招待あり
長野 直樹
月刊養殖ビジネス 56 ( 10 ) 4 - 7 2019年9月
記述言語：日本語 掲載種別：記事・総説・解説・論説等（商業誌、新聞、ウェブメディア） 出版者・発行元：緑書房
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佐賀玄海漁協の「唐津Qサバ」(佐賀県) 招待あり
長野 直樹
月刊養殖ビジネス 56 ( 10 ) 32 - 33 2019年9月
記述言語：日本語 掲載種別：記事・総説・解説・論説等（商業誌、新聞、ウェブメディア） 出版者・発行元：緑書房
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北浦養殖マサバ協業体の「ひむか本サバ」(宮崎県) 招待あり
長野 直樹
月刊養殖ビジネス 56 ( 10 ) 38 - 39 2019年9月
記述言語：日本語 掲載種別：記事・総説・解説・論説等（商業誌、新聞、ウェブメディア） 出版者・発行元：緑書房
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マサバ養殖における人工種苗の強みと課題． 招待あり
長野 直樹
月刊養殖ビジネス 56 ( 1 ) 36 - 38 2019年1月
記述言語：日本語 掲載種別：記事・総説・解説・論説等（商業誌、新聞、ウェブメディア）
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佐賀県唐津市におけるマサバの完全養殖とブランド化． 招待あり
長野 直樹
月刊養殖ビジネス 54 ( 1 ) 37 - 39 2017年1月
記述言語：日本語 掲載種別：記事・総説・解説・論説等（商業誌、新聞、ウェブメディア）
講演・口頭発表等 【 表示 ／ 非表示 】
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産学官連携による完全養殖マサバ（唐津Qサバ）の地域ブランド化 招待あり
長野直樹
和3年度日本水産学会九州支部例会 (福岡) 日本水産学会
開催年月日： 2021年9月4日
記述言語：日本語 会議種別：口頭発表（招待・特別）
開催地：福岡
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インライン近赤外分析によるマアジの自動脂質選別
那須春香, 河合慎吾, 田口智也, 古川三記子, 木宮隆, 長野直樹
令和2年度 日本水産学会春季大会 (東京) 日本水産学会
開催年月日： 2020年3月26日 - 2020年3月30日
記述言語：日本語 会議種別：ポスター発表
開催地：東京
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宮崎県日南市目井津港に水揚げされたマアジの脂質含量
漆間美季, 田口智也, 古川三記子, 長野直樹
令和2年度 日本水産学会春季大会 (東京) 日本水産学会
開催年月日： 2020年3月26日 - 2020年3月30日
記述言語：日本語 会議種別：ポスター発表
開催地：東京
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マサバの養殖技術開発とその課題 招待あり
長野直樹
全国サバ養殖フォーラム (大阪市) 水産研究・教育機構
開催年月日： 2019年11月16日
記述言語：日本語 会議種別：口頭発表（招待・特別）
開催地：大阪市
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Production of AVTR-V1a2 knockout strain in chub mackerel, Scomber japonicus, by genome editing with TALEN 国際会議
Sakanoue R, Ohga H, Shibata K, Nagano N, Kitano H, Sakaguchi K, Kuhara S, Tashiro K, Kim S, Nagasako T, Uchida S, Sakuma , Yamamoto T, Gen K, Fujiwara A, Kazeto Y, Kobayashi T, Ohta K, Matsuyama
Marine Biotechnology Conference 2019 (Japan) Marine Biotechnology Conference
開催年月日： 2019年9月9日 - 2019年9月13日
記述言語：英語 会議種別：ポスター発表
開催地：Japan
科研費（文科省・学振）獲得実績 【 表示 ／ 非表示 】
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完全養殖系とゲノム編集技術を用いた海産魚における新規育種基盤技術の開発
2015年04月 - 2019年03月
科学研究費補助金 基盤研究(A)
担当区分：研究分担者