MIYANISHI Hiroshi

写真a

Affiliation

Faculty of Agriculture Department of Marine Biological and Environmental Science

Title

Assistant Professor

External Link

Degree 【 display / non-display

  • 博士(理学) ( 2011.10   東京大学 )

  • 修士(教育学) ( 2007.3   横浜国立大学 )

  • 学士(教育) ( 2005.3   横浜国立大学 )

Research Areas 【 display / non-display

  • Life Science / Aquatic life science  / 魚類生理学:メダカ、サケ、ウナギ、チョウザメ、浸透圧調節、生殖、成長

 

Papers 【 display / non-display

  • Diel rhythm of the inflammatory cytokine il1b in the Japanese medaka (Oryzias latipes) regulated by core components of the circadian clock Reviewed

    Takeuchi T., Hata T., Miyanishi H., Yuasa T., Setoguchi S., Takeda A., Morimoto N., Hikima J.i., Sakai M., Kono T.

    Fish and Shellfish Immunology   127   238 - 246   2022.8

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Fish and Shellfish Immunology  

    In recent years, studies on circadian control in immunity have been actively conducted in mammals, but little is known about circadian rhythms in the field of fish immunology. In this study, we aimed to analyse the regulation of the diel oscillation of inflammatory cytokine interleukin-1β (il1b) gene expression by core components of the circadian clock in Japanese medaka (Oryzias latipes). The expression of il1b and clock genes (bmal1 and clock1) in medaka acclimated to a 12:12 light (L): dark (D) cycle showed diel rhythm. Additionally, higher expression of il1b was detected in medaka embryo cells (OLHdrR-e3) overexpressing bmal1 and clock1. A significant decrease in il1b expression was observed in OLHdrR-e3 cells after bmal1 knockdown using morpholino oligos. These changes may be mediated by transcriptional regulation via clock proteins, which target the E-box sequence in the cis-element of il1b as identified using luciferase reporter assays. Moreover, LPS stimulation and pathogenic bacterial infection at different zeitgeber time (ZT) under LD12:12 conditions affected the degree of il1b expression, which showed high and low responsiveness to both immuno-stimulations at ZT2 and ZT14, respectively. These results suggested that fish IL-1β exhibited diel oscillation regulated by clock proteins, and its responsiveness to immune-stimulation depends on the time of day.

    DOI: 10.1016/j.fsi.2022.06.020

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  • Reproductive farming technology in Japanese eel and chub mackerel Invited Reviewed

    Hiroshi Miyanishi, Naoki Nagano

    Aquaculture and Fisheries   7 ( 5 )   595 - 600   2022.4

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    Authorship:Lead author, Corresponding author   Language:Japanese   Publishing type:Research paper (scientific journal)  

    Japanese eel (Anguilla japonica) and chub mackerel (Scomber japonicus) are commercially valuable species in Asian aquaculture. The reduction in eel and chub mackerel resources has been a serious problem in recent years that should be addressed by reducing the catch of their natural populations to halt their decline and reach the Sustainable Development Goals. Furthermore, securing sufficient food supply by aquaculture is an important step in addressing the increasing demand for fish products in recent years. Traditionally, juveniles of the Japanese eel and chub mackerel have been captured to be raised in aquaculture. Owing to the extensive research on these species, new technologies have been developed for full-lifecycle aquaculture of Japanese eel in 2011 and chub mackerel in 2014. These technologies are expected to stop the decline of natural resources and provide a stable food supply. Recently, seed production of these species has increased owing to the development of successful broodstock management and larval rearing techniques. Fundamental information on oocyte maturation and ovulation and its application for artificial induction of sexual maturation is needed to produce good quality seeds of the Japanese eel and chub mackerel. Here, hormonal mechanisms and previously and newly developed methods for artificial seed production have been described.

    DOI: 10.1016/j.aaf.2022.03.003

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  • Thyroid and endostyle development in cyclostomes provides new insights into the evolutionary history of vertebrates Reviewed International coauthorship

    Wataru Takagi, Fumiaki Sugahara, Shinnosuke Higuchi, Rie Kusakabe, Juan Pascual-Anaya, Iori Sato, Yasuhiro Oisi, Nobuhiro Ogawa, Hiroshi Miyanishi, Noritaka Adachi, Susumu Hyodo, Shigeru Kuratani

    BMC Biology   20 ( 1 )   76   2022.4

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    Language:English   Publishing type:Research paper (scientific journal)  

    Background: The endostyle is an epithelial exocrine gland found in non-vertebrate chordates (amphioxi and tunicates) and the larvae of modern lampreys. It is generally considered to be an evolutionary precursor of the thyroid gland of vertebrates. Transformation of the endostyle into the thyroid gland during the metamorphosis of lampreys is thus deemed to be a recapitulation of a past event in vertebrate evolution. In 1906, Stockard reported that the thyroid gland in hagfish, the sister cyclostome group of lampreys, develops through an endostyle-like primordium, strongly supporting the plesiomorphy of the lamprey endostyle. However, the findings in hagfish thyroid development were solely based on this single study, and these have not been confirmed by modern molecular, genetic, and morphological data pertaining to hagfish thyroid development over the last century. Results: Here, we showed that the thyroid gland of hagfish undergoes direct development from the ventrorostral pharyngeal endoderm, where the previously described endostyle-like primordium was not found. The developmental pattern of the hagfish thyroid, including histological features and regulatory gene expression profiles, closely resembles that found in modern jawed vertebrates (gnathostomes). Meanwhile, as opposed to gnathostomes but similar to non-vertebrate chordates, lamprey and hagfish share a broad expression domain of Nkx2-1/2-4, a key regulatory gene, in the pharyngeal epithelium during early developmental stages. Conclusions: Based on the direct development of the thyroid gland both in hagfish and gnathostomes, and the shared expression profile of thyroid-related transcription factors in the cyclostomes, we challenge the plesiomorphic status of the lamprey endostyle and propose an alternative hypothesis where the lamprey endostyle could be obtained secondarily in crown lampreys.

    DOI: 10.1186/s12915-022-01282-7

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  • Interleukin-22 Deficiency Contributes to Dextran Sulfate Sodium-Induced Inflammation in Japanese Medaka, Oryzias latipes Reviewed

    Yoshie Takahashi, Yo Okamura, Nanaki Harada, Mika Watanabe, Hiroshi Miyanishi, Tomoya Kono, Masahiro Sakai, Jun Ichi Hikima

    Frontiers in Immunology   12   688036   2021.10

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    Language:English   Publishing type:Research paper (scientific journal)  

    Mucosal tissue forms the first line of defense against pathogenic microorganisms. Cellular damage in the mucosal epithelium may induce the interleukin (IL)-22-related activation of many immune cells, which are essential for maintaining the mucosal epithelial barrier. A previous study on mucosal immunity elucidated that mammalian IL-22 contributes to mucus and antimicrobial peptides (AMPs) production and anti-apoptotic function. IL-22 has been identified in several teleost species and is also induced in response to bacterial infections. However, the roles of IL-22 in teleost immunity and mucus homeostasis are poorly understood. In this study, Japanese medaka (Oryzias latipes) was used as a model fish. The medaka il22, il22 receptor A1 (il22ra1), and il22 binding protein (il22bp) were cloned and characterized. The expression of medaka il22, il22ra1, and il22bp in various tissues was measured using qPCR. These genes were expressed at high levels in the mucosal tissues of the intestines, gills, and skin. The localization of il22 and il22bp mRNA in the gills and intestines was confirmed by in situ hybridizations. Herein, we established IL-22-knockout (KO) medaka using the CRISPR/Cas9 system. In the IL-22-KO medaka, a 4-bp deletion caused a frameshift in il22. To investigate the genes subject to IL-22-dependent regulation, we compared the transcripts of larval medaka between wild-type (WT) and IL-22-KO medaka using RNA-seq and qPCR analyses. The comparison was performed not only in the naïve state but also in the dextran sulfate sodium (DSS)-exposed state. At the transcriptional level, 368 genes, including immune genes, such as those encoding AMPs and cytokines, were significantly downregulated in IL-22-KO medaka compared that in WT medaka in naïve states. Gene ontology analysis revealed that upon DSS stimulation, genes associated with cell death, acute inflammatory response, cell proliferation, and others were upregulated in WT medaka. Furthermore, in DSS-stimulated IL-22-KO medaka, wound healing was delayed, the number of apoptotic cells increased, and the number of goblet cells in the intestinal epithelium decreased. These results suggested that in medaka, IL-22 is important for maintaining intestinal homeostasis, and the disruption of the IL-22 pathway is associated with the exacerbation of inflammatory pathology, as observed for mammalian IL-22.

    DOI: 10.3389/fimmu.2021.688036

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  • Identification and expression of phospholipase A2 genes related to transcriptional control in the interleukin-17A/F1 pathway in the intestines of Japanese medaka Oryzias latipes Reviewed

    Okamura Y, Miyanishi H, Kono T, Sakai M, Hikima J.

    Fish and Shellfish Immunology Reports   in press   2021.9

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    Language:English   Publishing type:Research paper (scientific journal)  

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MISC 【 display / non-display

  • 川が好きなメダカと海が好きな?メダカ Invited

    宮西 弘

    月刊アクアライフ   9月号増刊   270 - 271   2022.9

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    Authorship:Corresponding author   Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media)   Publisher:エムビージェー  

  • 川が好きなメダカと海が好きな?メダカ Invited

    宮西 弘

    月刊アクアライフ   43 ( 5 )   80 - 81   2021.4

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    Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media)   Publisher:エムビージェー  

Presentations 【 display / non-display

  • メダカにおける密度認識と高密度飼育による成長阻害

    藤城耕陽、宮西弘

    日本水産学会 秋季大会  2022.9.5 

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    Event date: 2022.9.5 - 2022.9.7

    Language:Japanese   Presentation type:Poster presentation  

  • 温暖海域に対応した温度耐性ヤマメ種苗の作出に資する基礎研究

    山徳知夏、上野賢、宮西弘、内田勝久

    日本水産学会 秋季大会  2022.9.6 

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    Event date: 2022.9.5 - 2022.9.7

    Language:Japanese   Presentation type:Oral presentation (general)  

  • 2系統ヤマメの銀化発動および海水適応の比較

    南祐人、上野賢、宮西弘、内田勝久

    日本水産学会 秋季大会  2022.9.6 

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    Event date: 2022.9.5 - 2022.9.7

    Language:Japanese   Presentation type:Oral presentation (general)  

  • 軟骨魚類トラザメの塩類細胞における転写因子forkhead box I3の発現 Invited

    宮西 弘・西 真理奈・高木 亙・井ノ口 繭・兵藤 晋・内田 勝久

    板鰓類シンポジウム2021  2021.12.17 

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    Event date: 2021.12.17

    Language:Japanese   Presentation type:Oral presentation (general)  

  • メダカの高浸透圧処理によるカルシウム活性化クロライドチャネルAnoctamin1の発現と局在

    今野紀文、富樫彩音、宮西弘、中町智哉、松田恒平

    日本比較内分泌学会  2021.11 

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    Event date: 2021.12.12 - 2021.12.14

    Language:Japanese   Presentation type:Poster presentation  

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Awards 【 display / non-display

  • 第24回バゾプレシン研究会 研究奨励賞

    2014.1   バゾプレシン研究会  

    宮西弘

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    Award type:Award from Japanese society, conference, symposium, etc.  Country:Japan

  • 第4回 東京大学大気海洋研究所 博士課程修了者 所長賞

    2012.3   東京大学 大気海洋研究所  

    宮西弘

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    Country:Japan

  • 第35回日本比較内分泌学会大会 若手研究者最優秀発表賞

    2010.11   日本比較内分泌学会  

    宮西弘 他

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    Award type:Award from Japanese society, conference, symposium, etc.  Country:Japan

  • The 16th International Congress of Comparative Endocrinology Travel award

    2009.6   International Federation of Comparative Endocrinological Societies (IFCES)  

    宮西弘 他

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    Award type:Award from international society, conference, symposium, etc.  Country:Japan

Grant-in-Aid for Scientific Research 【 display / non-display

  • 魚類塩類細胞分化誘導シグナリングの解明から迫る広塩性決定因子の同定と応用

    Grant number:19K15912  2019.04 - 2022.03

    科学研究費補助金  若手研究

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    Authorship:Principal investigator 

  • 新規海水適応遺伝子の探索および海水型塩類細胞分化誘導因子の同定

    2014.04 - 2017.03

    科学研究費補助金  若手研究(B)

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    Authorship:Principal investigator