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Affiliation |
Faculty of Agriculture Region of Marine Life Science |
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Title |
Associate Professor |
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External Link |
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Related SDGs |
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MIYANISHI Hiroshi
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Degree 【 display / non-display 】
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博士（理学） ( 2011.10 東京大学 )
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修士（教育学） ( 2007.3 横浜国立大学 )
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学士（教育） ( 2005.3 横浜国立大学 )
Research Areas 【 display / non-display 】
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Life Science / Aquatic life science / 魚類生理学：メダカ、サケ、ウナギ、チョウザメ、浸透圧調節、生殖、成長
Papers 【 display / non-display 】
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Konno N., Togashi A., Miyanishi H., Azuma M., Nakamachi T., Matsuda K.
Journal of Experimental Zoology Part A: Ecological and Integrative Physiology in press 2024.12
Language：English Publishing type：Research paper (scientific journal) Publisher：Journal of Experimental Zoology Part A: Ecological and Integrative Physiology
In euryhaline teleosts, the cystic fibrosis transmembrane conductance regulator (CFTR) in seawater (SW)-type chloride cells facilitates apical Cl− secretion for SW adaptation, while alternative Cl− excretion pathways remain understudied. This study investigates the role of the calcium-activated chloride channel, Anoctamin 1 (ANO1), in the gills of the euryhaline Japanese medaka (Oryzias latipes) under hyperosmolality and cortisol (CORT) influence. Acclimation to artificial SW, NaCl, mannitol, or glucose significantly upregulated ANO1 and CFTR mRNA expression in gills, unlike urea treatment. In situ hybridization revealed ANO1 mRNA in chloride cells co-expressing CFTR and Na+, K+-ATPase under hyperosmotic conditions. ANO1 inhibition elevated plasma Cl− concentration, indicating impaired Cl− excretion. CORT or dexamethasone administration in freshwater (FW) fish significantly increased branchial ANO1 and CFTR mRNA expression, an effect attenuated by the glucocorticoid receptor (GR) antagonist RU486. Hyperosmotic treatment of isolated gill tissues rapidly induced ANO1 mRNA expression independent of CFTR mRNA changes, and this induction was unaffected by RU486. These findings highlight the dual regulation of ANO1 expression via hyperosmolality-induced cellular response and the CORT–GR system. Thus, branchial ANO1 may likely complement CFTR in Cl⁻ excretion, playing a key role in the hyperosmotic adaptation of euryhaline teleosts.
DOI： 10.1002/jez.2894
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Yamamoto J, Deguchi H, Sumiyoshi T, Nakagami K, Saito A, Miyanishi H, Kondo M, Kono T, Sakai M, Kinoshita M, Hikima JI
Marine biotechnology (New York, N.Y.) 26 ( 4 ) 658 - 671 2024.6
Language：English Publishing type：Research paper (scientific journal) Publisher：Marine Biotechnology
Intracellular bacteria such as those belonging to the genus Edwardsiella can survive and proliferate within macrophages. However, the detailed mechanisms underlying the host macrophage immune response and pathogen evasion strategies remain unknown. To advance the field of host macrophage research, we successfully established transgenic (Tg) Japanese medaka Oryzias latipes that possesses fluorescently visualized macrophages. As a macrophage marker, the macrophage-expressed gene 1.1 (mpeg1.1) was selected because of its predominant expression across various tissues in medaka. To validate the macrophage characteristics of the fluorescently labeled cells, May-Grünwald Giemsa staining and peroxidase staining were conducted. The labeled cells exhibited morphological features consistent with those of monocyte/macrophage-like cells and tested negative for peroxidase activity. Through co-localization studies, the fluorescently labeled cells co-localized with E. piscicida in the intestines and kidneys of infected medaka larvae, confirming the ingestion of bacteria through phagocytosis. In addition, the labeled cells expressed macrophage markers but lacked a neutrophil marker. These results suggested that the fluorescently labeled cells of Tg[mpeg1.1:mCherry/mAG] medaka were monocytes/macrophages, which will be useful for future studies aimed at understanding the mechanisms of macrophage-mediated bacterial infections.
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Fujishiro K., Miyanishi H.
Zoological Science 40 ( 5 ) 404 - 413 2023.7
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：Zoological Science
High stocking densities have negative effects on fish. However, the mechanism mediating density perception and growth inhibition is still unknown. This study was conducted to confirm the occurrence of growth inhibition and evaluate changes in growth-related factors in fish reared under high-stocking-density conditions and to determine the role of vision in density perception of medaka. In the graduated-stocking experiment, growth inhibition was clearly observed in fish reared at higher densities, although environmental factors, such as water quality, dissolved oxygen, and feeding conditions, were the same in each experimental group. Differences in growth were observed between the 6-fish and 8-fish groups, indicating that medaka have a superior sense that allows them to accurately perceive the number of individuals in their surroundings. In the pseudo-high stocking experiment, the inner 2-L tank in both groups contained six fish; however, the outer 3-L tank in the pseudo group contained several fish, while that of the control group contained only water. Growth inhibition was observed among the fish in the inner tank of the pseudo group despite having similar spatial density with the control group. These findings suggest that vision is important for density perception. The gene expression of growth-related and metabolic-regulatory hormones decreased in the high-density group. Furthermore, neuropeptide Y expression increased, while pro-opiomelanocortin expression decreased in the high-density group. This study is the first to report that fish can visually perceive density and the resulting growth inhibition, and concluded that medaka is a suitable model for studying density effects and perception in fish. .
DOI： 10.2108/zs230018
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キャビアサイクルの短縮化
宮西 弘
調査月報 361 2 - 6 2023
Publishing type：Research paper (scientific journal)
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Takeuchi T., Hata T., Miyanishi H., Yuasa T., Setoguchi S., Takeda A., Morimoto N., Hikima J.i., Sakai M., Kono T.
Fish and Shellfish Immunology 127 238 - 246 2022.8
Language：English Publishing type：Research paper (scientific journal) Publisher：Fish and Shellfish Immunology
In recent years, studies on circadian control in immunity have been actively conducted in mammals, but little is known about circadian rhythms in the field of fish immunology. In this study, we aimed to analyse the regulation of the diel oscillation of inflammatory cytokine interleukin-1β (il1b) gene expression by core components of the circadian clock in Japanese medaka (Oryzias latipes). The expression of il1b and clock genes (bmal1 and clock1) in medaka acclimated to a 12:12 light (L): dark (D) cycle showed diel rhythm. Additionally, higher expression of il1b was detected in medaka embryo cells (OLHdrR-e3) overexpressing bmal1 and clock1. A significant decrease in il1b expression was observed in OLHdrR-e3 cells after bmal1 knockdown using morpholino oligos. These changes may be mediated by transcriptional regulation via clock proteins, which target the E-box sequence in the cis-element of il1b as identified using luciferase reporter assays. Moreover, LPS stimulation and pathogenic bacterial infection at different zeitgeber time (ZT) under LD12:12 conditions affected the degree of il1b expression, which showed high and low responsiveness to both immuno-stimulations at ZT2 and ZT14, respectively. These results suggested that fish IL-1β exhibited diel oscillation regulated by clock proteins, and its responsiveness to immune-stimulation depends on the time of day.
MISC 【 display / non-display 】
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サケ・マス類における海水経験法による成長促進 Invited
宮西 弘
月刊養殖ビジネス 61 ( 3 ) 42 - 46 2024.3
Authorship：Lead author Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media)
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キャビア生産サイクルの短縮化 Invited
宮西 弘
調査月報 361 2 - 6 2023.5
Authorship：Lead author Language：Japanese Publishing type：Internal/External technical report, pre-print, etc.
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川が好きなメダカと海が好きな？メダカ２ Invited
宮西 弘
月刊アクアライフ 9月号増刊 270 - 271 2022.9
Authorship：Corresponding author Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media) Publisher：エムビージェー
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川が好きなメダカと海が好きな？メダカ Invited
宮西 弘
月刊アクアライフ 43 ( 5 ) 80 - 81 2021.4
Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media) Publisher：エムビージェー
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総論 : 海洋生物の適応戦略－新規技術・現象からの新展開－ Invited
御輿 真穂, 宮西弘, 兵藤 晋
月刊海洋 49 183 - 186 2017.3
Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media)
Presentations 【 display / non-display 】
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時計遺伝子Bmal1変異メダカにおける免疫応答
羽田崇彦 ・平吉美優・津田和忠・宮西弘・引間順一・河野智哉
令和6年度日本水産学会 秋季大会
Event date： 2024.9.24 - 2024.9.27
Presentation type：Oral presentation (general)
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チョウザメにおける海水経験法による成長促進
宮西弘・濵嵜菜々子・松島悠一郎
令和６年度日本水産学会 秋季大会
Event date： 2024.9.24 - 2024.9.27
Presentation type：Oral presentation (general)
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ニジマスの高密度飼育による成長および脳内遺伝子発現への影響
藤城耕陽・西川颯太朗・宮西弘
令和６年度日本水産学会 秋季大会
Event date： 2024.9.24 - 2024.9.27
Presentation type：Oral presentation (general)
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Medaka (Oryzias latipes) is a suitable model for studying density effects and perception in fish International conference
Kouyou Fujishiro, Hiroshi Miyanishi
Joint International Symposium on Fisheries Oceanography with University of Miyazaki and Gyeongsang National University 2023.12
Event date： 2023.12.17 - 2023.12.20
Language：English Presentation type：Oral presentation (general)
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高密度飼育および認識によるメダカ脳内遺伝子発現変化とfos遺伝子の解析
藤城耕陽、宮西弘
令和5年度日本水産学会九州支部大会 2023.12.9
Event date： 2023.12.9
Language：Japanese Presentation type：Oral presentation (general)
Awards 【 display / non-display 】
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地域貢献表彰 最優秀賞
2024.12 宮崎大学農学部 チョウザメにおけるキャビア生産効率向上に資する産学官連携研究
宮西弘
Award type：International academic award (Japan or overseas)
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学生優秀発表賞
2023.12 日本水産学会九州支部 高密度飼育および認識によるメダカ脳内遺伝子発現変化とfos遺伝子の解析
藤城耕陽・宮西弘
Award type：Award from Japanese society, conference, symposium, etc.
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最優秀発表賞
2023.8 日本下垂体研究会 メダカにおける密度認識と高密度飼育下での成長阻害および内分泌学的解析
藤城耕陽・宮西弘
Award type：International academic award (Japan or overseas)
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学生優秀発表賞
2023.1 日本水産学会九州支部 単一細胞レベルトランスクリプトーム解析による魚類の鰓塩類細胞分化因子の探索
谷口雄真・宮西弘
Award type：Award from Japanese society, conference, symposium, etc.
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第24回バゾプレシン研究会 研究奨励賞
2014.1 バゾプレシン研究会
宮西弘
Award type：Award from Japanese society, conference, symposium, etc. Country：Japan
Grant-in-Aid for Scientific Research 【 display / non-display 】
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海水経験技術による回遊性水産有用魚種の成長・成熟促進機構の解明と応用
Grant number：23K26989 2023.04 - 2027.03
日本学術振興会 科学研究費基金 基盤研究(B)
宮西 弘
Authorship：Principal investigator
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海水経験技術による回遊性水産有用魚種の成長・成熟促進機構の解明と応用
Grant number：23H02296 2023.04 - 2027.03
日本学術振興会 科学研究費補助金 基盤研究(B)
宮西 弘
Authorship：Principal investigator
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魚類の高密度飼育による成長阻害因子の同定および高密度耐性魚の作出
Grant number：22K19216 2022.06 - 2025.03
日本学術振興会 科学研究費基金 挑戦的研究(萌芽)
宮西 弘
Authorship：Principal investigator
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魚類塩類細胞分化誘導シグナリングの解明から迫る広塩性決定因子の同定と応用
Grant number：19K15912 2019.04 - 2022.03
科学研究費補助金 若手研究
Authorship：Principal investigator
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新規海水適応遺伝子の探索および海水型塩類細胞分化誘導因子の同定
Grant number：26850130 2014.04 - 2017.03
科学研究費補助金 若手研究(B)
Authorship：Principal investigator
Available Technology 【 display / non-display 】
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魚の水環境における適応機構と飼育密度の研究
海水経験技術による成長の促進と海水への適応能力の向上
光環境操作によるウナギの成熟促進技術の開発Home Page： 海洋生物環境学科・教員紹介（宮西弘）
Related fields where technical consultation is available：・魚に適した環境を整え、健康に飼う方法
・魚を大きくする方法Message：・魚を健康に大きく育てたいなど、様々な相談があればご連絡下さい。
・遺伝子操作などの先端的な研究手法から飼育まで幅広く研究を行っていますので、何でもご相談下さい。