宮西 弘 (ミヤニシ ヒロシ)

MIYANISHI Hiroshi

写真a

職名

助教

研究室住所

宮崎県宮崎市学園木花台西1-1 S405

研究分野・キーワード

魚類生理学

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メールアドレス

研究室電話

0985-58-7223

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  • 東京大学 -  博士(理学)

  • 横浜国立大学 -  修士(教育学)

  • 横浜国立大学 -  学士(教育)

 

論文 【 表示 / 非表示

  • The activin signaling transcription factor Smox is an essential regulator of appendage size during regeneration after autotomy in the crayfish

    Shinji J., Gotoh H., Miyanishi H., Lavine M., Lavine L.

    Evolution and Development   21 ( 1 ) 44 - 55   2019年01月

    共著

     概要を見る

    © 2018 Wiley Periodicals, Inc. Members of the phylum Arthropoda, comprising over 80% of total animal species, have evolved regenerative abilities, but little is known about the molecular mechanisms mediating this process. Transforming growth factor β (TGF-β) signaling mediates a diverse set of essential processes in animals and is a good candidate pathway for regulation of regeneration in arthropods. In this study we investigated the role of activin signaling, a TGF-β superfamily pathway, in limb regeneration in the crayfish. We identified and cloned a downstream transcription factor in the activin pathway, Smox, and characterized its function with regard to other elements of the activin signaling pathway. Gene knockdown of Smox by RNAi induced regeneration of complete but smaller pereopods after autotomy. This indicates that activin signaling via Smox functions in regulation of pereopod growth and size. The expression levels of both Smox and the activin receptor babo were closely correlated with molting. The expression level of Smox increased when babo was knocked down by RNAi, indicating that Smox and babo transcription are linked. Our study suggests that the Babo-Smox system in activin signaling is conserved in decapods, and supports an evolutionary conservation of this aspect of molecular signaling during regeneration between protostomes and deuterostomes.

    DOI PubMed

  • Functional classification of gill ionocytes and spatiotemporal changes in their distribution after transfer from seawater to freshwater in Japanese seabass

    Inokuchi M., Nakamura M., Miyanishi H., Hiroi J., Kaneko T.

    Journal of Experimental Biology   220 ( 24 ) 4720 - 4732   2017年12月

    共著

     概要を見る

    © 2017. Published by The Company of Biologists Ltd . Spatiotemporal changes in branchial ionocyte distribution were investigated following transfer from seawater (SW) to freshwater (FW) in Japanese seabass. The mRNA expression levels of cystic fibrosis transmembrane conductance regulator (CFTR) and Na+//K+// 2Cl- cotransporter 1a (NKCC1a) in the gills rapidly decreased after transfer to FW, whereas Na+/H+ exchanger 3 (NHE3) and Na+/Cl- cotransporter 2 (NCC2) expression were upregulated following the transfer. Using quadruple-color whole-mount immunofluorescence staining with anti-Na+/K+/-ATPase, anti-NHE3, anti-CFTR and T4 (anti-NKCC1a/NCC2) antibodies, we classified ionocytes into one SW type and two FW types: NHE3 cell and NCC2 cell. Time course observation after transfer revealed an intermediate type between SWtype and FW-Type NHE3 ionocytes, suggesting functional plasticity of ionocytes. Finally, on the basis of the ionocyte classification of Japanese seabass, we observed the location of ionocyte subtypes on frozen sections of the gill filaments stained by triple-color immunofluorescence staining. Our observation indicated that SWtype ionocytes transformed into FW-Type NHE3 ionocytes and at the same time shifted their distribution from filaments to lamellae. However, FW-specific NCC2 ionocytes appeared mainly in the filaments. Taken together, these findings indicate that ionocytes originated from undifferentiated cells in the filaments and expanded their distribution to the lamellae during FW acclimation.

    DOI PubMed

  • 広塩性魚におけるin vivoコンディショナルノックダウンを用いた塩類細胞分化機構の解明

    宮西 弘, 高屋敷 早紀, 金子 豊二

    月刊海洋   49   202 - 207   2017年03月  [招待有り]

    共著

  • 総論 : 海洋生物の適応戦略-新規技術・現象からの新展開-

    御輿 真穂, 宮西弘, 兵藤 晋

    月刊海洋   49 ( 4 ) 183 - 186   2017年03月  [招待有り]

    共著

  • Past seawater experience enhances seawater adaptability in medaka, Oryzias latipes.

    Miyanishi H, Inokuchi M, Nobata S, Kaneko T

    Zoological letters   2   12   2016年

    共著

    DOI PubMed

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科研費(文科省・学振)獲得実績 【 表示 / 非表示

  • 魚類塩類細胞分化誘導シグナリングの解明から迫る広塩性決定因子の同定と応用

    若手研究

    研究期間:  2019年04月  -  2022年03月  代表者:  宮西弘

  • 新規海水適応遺伝子の探索および海水型塩類細胞分化誘導因子の同定

    若手研究(B)

    研究期間:  2014年04月  -  2017年03月  代表者:  宮西弘

その他競争的資金獲得実績 【 表示 / 非表示

  • 魚類の高塩分味覚受容機構の解明と回遊現象との関連

    提供機関:  民間財団等  稲盛研究助成

    研究期間: 2019年04月  -  2020年03月  代表者:  宮西弘

  • 浸透圧調節を担う塩類細胞における分子基盤の構築

    提供機関:  民間財団等  ノバルティス研究奨励金

    研究期間: 2018年04月  -  2019年03月  代表者:  宮西弘