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Affiliation |
Faculty of Agriculture Region of Applied Biochemistry and Biotechnology |
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Title |
Assistant Professor |
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External Link |
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Related SDGs |
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KIYOSHI Keiji
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Degree 【 display / non-display 】
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醸造学 ( 2016.3 東京農業大学 )
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Fermentation technology ( 2012.3 Tokyo University of Agriculture )
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Fermentation technology ( 2010.3 Tokyo University of Agriculture )
Research Areas 【 display / non-display 】
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Life Science / Applied microbiology
Papers 【 display / non-display 】
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Physiological role of the <i>EHL</i> gene in sake yeast and its effects on quality of sake Reviewed
Seibutsu-kogaku Kaishi 104 ( 2 ) 54 - 54 2026.2
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：The Society for Biotechnology, Japan
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Mochammad W., Fukunaga T., Norsingha P., Nagamine M., Kiyoshi K., Inoue K., Okuyama Y.
ACS Sustainable Resource Management 2 ( 8 ) 1373 - 1378 2025.8
Publishing type：Research paper (scientific journal) Publisher：ACS Sustainable Resource Management
A hydrogen sensor utilizing proton-conducting ceramics was investigated for real-time monitoring of biohydrogen produced from soybean food wastewater using Clostridium neuense strain SDL48. Biohydrogen production began approximately 42 h after inoculation with strain SDL48, eventually reaching a concentration where hydrogen accounted for 43% of the gas phase. Additionally, the biohydrogen was purified using proton-conducting ceramics, successfully yielding hydrogen of high purity, qualifying it as pure hydrogen.
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Tofu whey treatment and electricity generation by stacked microbial fuel cells Reviewed
Mochammad W., Fujimura S., Shichida M., Nagamine M., Kiyoshi K., Inoue K.
Journal of Environmental Chemical Engineering 13 ( 3 ) 2025.6
Publishing type：Research paper (scientific journal) Publisher：Journal of Environmental Chemical Engineering
Tofu whey, a sugar- and protein-rich liquid waste generated during tofu production, was evaluated as a substrate for stacked microbial fuel cells (SMFCs) in this study. Two types of tofu whey were used: Japanese tofu whey, with a near-neutral pH, and Indonesian tofu whey, which is acidic. The SMFCs were operated in two different modes: circulation mode and flow-through mode. In circulation mode, the highest power densities achieved were 3.03 Wm-3 for Japanese tofu whey and 2.75 Wm-3 for Indonesian tofu whey at an external resistance of 1000 ω. In flow-through mode, power densities reached 13.1 Wm-3 at 51 ω for Japanese tofu whey and 4.88 Wm-3 at 100 ω for Indonesian tofu whey. Across all SMFCs, chemical oxygen demand, sugar, and protein content decreased during the operation, while pH, nitrate concentration, and electrolyte conductivity increased. Microbial community analysis revealed that Lactobacillales, Flavobacteriales, and Enterobacteriales became predominant in SMFCs using Japanese tofu whey after the operation, while Burkholderiales and Flavobacteriales were predominant in SMFCs using Indonesian tofu whey. This study set new benchmarks for power density in SMFCs and demonstrated the effectiveness of treating Japanese and Indonesian tofu whey, with superior performance in flow-through mode.
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Tofu whey treatment and electricity generation by stacked microbial fuel cells Reviewed
Wachid Mochammad, Soichiro Fujimura, Misaki Shichida, Miyuki Nagamine, Keiji Kiyoshi, Kengo Inoue
Journal of Environmental Chemical Engineering 13 ( 3 ) 2025.3
Language：English Publishing type：Research paper (scientific journal)
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Physiological role of the EHL gene in sake yeast and its effects on quality of sake. Reviewed
Tomonaga K, Tanaka J, Kiyoshi K, Akao T, Watanabe K, Kadokura T, Nakayama S
Journal of bioscience and bioengineering 137 ( 3 ) 195 - 203 2024.3
Language：English Publishing type：Research paper (scientific journal) Publisher：Journal of Bioscience and Bioengineering
The EHL1/2/3 genes were identified by whole-genome sequencing of Kyokai No. 7 (K7), which is a well-known representative Japanese sake yeast Saccharomyces cerevisiae. The genes are present in K7, but not in laboratory strain S288C. Although the genes were presumed to encode epoxide hydrolase based on homology analysis, their effect on cellular metabolism in sake yeast has not yet been clarified. We constructed ehl1/2/3 mutants harboring a stop codon in each gene using the haploid yeast strain H3 as the parental strain, which was derived from K701, and investigated the physiological role and effects of the EHL1/2/3 genes on sake quality. Metabolome analysis and vitamin requirement testing revealed that the EHL1/2/3 genes are partly responsible for the synthesis of pantothenate. For fermentation profiles, ethanol production by the ehl1/2/3 mutant was comparable with that of strain H3, but succinate production was decreased in the ehl1/2/3 mutant compared to strain H3 when cultured in yeast malt (YM) medium containing 10% glucose and during sake brewing. Ethyl hexanoate and isoamyl acetate levels in the ehl1/2/3 mutant strain were decreased compared to those of strain H3 during sake brewing. Thus, the EHL1/2/3 genes did not affect ethanol production but did affect the production of organic acids and aromatic components during sake brewing.
Presentations 【 display / non-display 】
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Citrobacter braakii TB-96株における陰極電気発酵を用いた1,3-プロパンジオール生産の強化
柳瀬卓馬，井上謙吾，吉田ナオト，中島（神戸）敏明，清 啓自
第31回日本生物工学会九州支部熊本大会 2026.12.6
Event date： 2026.12.6
Language：Japanese Presentation type：Oral presentation (general)
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アンモニアガス存在下におけるKlebsiella属細菌の生育特性と分離株 (RD株) の窒素固定能の解析
柳瀬卓馬，井上謙吾，吉田ナオト，中島（神戸）敏明，清 啓自
第31回日本生物工学会九州支部熊本大会 2026.12.6
Event date： 2026.12.6
Language：Japanese Presentation type：Oral presentation (general)
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1 3 プロパンジオール生産性 Citrobacter braakii TB-96 の育種による代謝改変
木村 武蔵, 鮫島 礼, 柳瀬 卓馬, 吉田 ナオト, 中島（神戸） 敏明, 清 啓自
第30回 日本生物工学会九州支部宮崎大会 2025.12.1
Event date： 2025.12.1
Language：Japanese Presentation type：Oral presentation (general)
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タンパク質を基質としたアンモニア生成菌のスクリーニング法
川原 朋、吉田 ナオト、清 啓自
第30回 日本生物工学会九州支部宮崎大会 2025.12.1
Event date： 2025.12.1
Language：Japanese Presentation type：Oral presentation (general)
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Klebsiella sp. RD のアンモニアガス中での生育及び窒素固定の可能性
吉道 達海, 清 啓自, 山本 昭洋, 佐伯 雄一, 吉田 ナオト
第77回 日本生物工学会大会 2025.9.11
Event date： 2025.9.10 - 2025.9.12
Language：Japanese Presentation type：Oral presentation (general)
Awards 【 display / non-display 】
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第33回 生物工学論文賞
2025.9 日本生物工学会
友永佳津子, 田中純平, 清啓自, 赤尾健, 渡邉康太, 門倉利守, 中山俊一
Award type：Award from Japanese society, conference, symposium, etc.
Grant-in-Aid for Scientific Research 【 display / non-display 】
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微生物の発酵をコントロールする栄養たち
Grant number：23HT0192 2023.04 - 2024.03
独立行政法人日本学術振興会 科学研究費補助金 ひらめきときめきｻｲｴﾝｽ
Authorship：Principal investigator
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核酸代謝の増強が環境ストレスへの対応に及ぼす影響
Grant number：21K14773 2021.04 - 2024.03
独立行政法人日本学術振興会 科学研究費補助金 若手研究
Authorship：Principal investigator
全ての生物は自身の周辺環境から受けるストレスに適応しながら活動、生存し繁殖していく。環境から受けるストレスとは避けられないものであり、そこへ如何に適応できるのかが結果として生物の
種の強さを決定づけているといえる。これは単細胞生物である微生物においては尚更であり、特に発酵に用いられる微生物では如何にこのストレスによる発酵の低下を避けるかは生産性を左右する極めて重要な課題である。外的ストレスが過剰であると微生物は休眠化あるいは細胞死が生じる。
本研究では発酵性の微生物において申請者がこれまでに発見した培地による核酸供給による
熱ストレス耐性の知見を基に、ストレス環境下における核酸などの主要代謝物の外的な供給および生合成の強化によって、いかなるストレスに対してもストレス耐性を付与することが可能であるかを分子生物学的アプローチによって試みる。 -
バイオ燃料廃棄物系バイオマスからポリマー原料への微生物転換
Grant number：99999999 2017.04 - 2019.03
科学研究費補助金 科学技術振興機構 先端的技術開発
Authorship：Coinvestigator(s)
Available Technology 【 display / non-display 】
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農業廃棄物からのブタノール生産
バイオディーゼル燃料の副産物からのアルコール生産
木質が微生物発酵に及ぼす影響の解明Home Page： 微生物機能開発学研究室：
Related fields where technical consultation is available：酒類や発酵食品の醸造技術、様々な原料の微生物への発酵利用
Message：微生物の発酵技術は、様々な物質生産へと応用できます。食品の産業廃棄物など現在利用されていない資源の有効活用法などで微生物の活用を検討の際にお気軽にご相談ください。(清: kiyoshi.keiji.p8@cc.miyazaki-u.ac.jp)