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Affiliation |
Organization for Promotion of Research and Industry-Academic Regional Collaboration Division for Intellectual Property and Research Risk Management |
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Title |
Assistant Professor |
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Laboratory Phone number |
0985-58-7592 |
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External Link |
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NAGAHAMA Hideki
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Research Areas 【 display / non-display 】
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Others / Others / Intellectual property、 Technology transfer
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Life Science / Molecular biology / Molecular genetics
External Career 【 display / non-display 】
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Kyushu University
2019.4
Country：Japan
Papers 【 display / non-display 】
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Fine Particle Adsorption Capacity of Volcanic Soil from Southern Kyushu, Japan. Reviewed International journal
Misawa N, Yasui K, Sakai K, Kobayashi T, Nagahama H, Haraguchi T, Sasaki S, Torrung V, Luangtongkum T, Taniguchi T, Yamada K, Minamimagari M, Usami T, Kinoshita H
Nanomaterials (Basel, Switzerland) 13 ( 3 ) 2023.1
Language：English Publishing type：Research paper (scientific journal)
DOI： 10.3390/nano13030568
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A quantitative protocol for DNA metabarcoding of springtails (Collembola). Reviewed
Saitoh S, Aoyama H, Fujii S, Sunagawa H, Nagahama H, Akutsu M, Shinzato N, Kaneko N, Nakamori T
Genome 59 ( 9 ) 705 - 23 2016.9
Language：English Publishing type：Research paper (scientific journal) Publisher：Genome
© 2016 Published by NRC Research Press. We developed a novel protocol with superior quantitative analysis results for DNA metabarcoding of Collembola, a major soil microarthropod order. Degenerate PCR primers were designed for conserved regions in the mitochondrial cytochrome c oxidase subunit I (mtCOI) and 16S ribosomal RNA (mt16S) genes based on published collembolan mitogenomes. The best primer pair was selected based on its ability to amplify each gene, irrespective of the species. DNA was extracted from 10 natural communities sampled in a temperate forest (with typically 25-30 collembolan species per 10 soil samples) and 10 mock communities (with seven cultured collembolan species). The two gene regions were then amplified using the selected primers, ligated with adapters for 454 technology, and sequenced. Examination of the natural community samples showed that 32 and 36 operational taxonomic units (defined at a 90% sequence similarity threshold) were recovered from the mtCOI and mt16S data, respectively, which were comparable to the results of the microscopic identification of 25 morphospecies. Further, sequence abundances for each collembolan species from the mtCOI and mt16S data of the mock communities, after normalization by using a species as the internal control, showed good correlation with the number of individuals in the samples (R = 0.91-0.99), although relative species abundances within a mock community sample estimated from sequences were skewed from community composition in terms of the number of individuals or biomass of the species. Thus, this protocol enables the comparison of collembolan communities in a quantitative manner by metabarcoding.
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A rapid method of non-destructive DNA extraction from individual springtails (Collembola) Reviewed
Aoyama H., Saitoh S., Fujii S., Nagahama H., Shinzato N., Kaneko N., Nakamori T.
Applied Entomology and Zoology 50 ( 3 ) 419 - 425 2015.8
Language：English Publishing type：Research paper (scientific journal) Publisher：Applied Entomology and Zoology
© 2015, The Japanese Society of Applied Entomology and Zoology. In this study, we describe an easy and rapid method for non-destructive DNA extraction from a single Collembola individual, without dissection, lysis of the specimen, or purification of extracted DNA. We demonstrate that, after a single specimen has been heat-treated in Tris-EDTA (TE) buffer using a standard thermocycler, the solution can be used for PCR amplification of the mitochondrial cytochrome c oxidase subunit 1 (COI) gene region, typically used for DNA barcoding. With this method, the morphological features of Collembola commonly used for species identification are well preserved. This DNA extraction method is preferable for DNA barcoding where the sequencing and preservation of a large number of small and fragile specimens are required.
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DNA metabarcoding of springtails (Collembola) Reviewed
Saitoh, Seikoh; Aoyama, Hiroaki; Fujii, Saori; Sunagawa, Haruki; Nagahama, Hideki; Akutsu, Masako; Shinzato, Naoya; Kaneko, Nobuhiro; Nakamori, Taizo;
GENOME 58 ( 5 ) 274 - 274 2015
Language：English Publishing type：Research paper (scientific journal)
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Shiba Y, Miyagawa H, Nagahama H, Matsumoto K, Kondo D, Matsuoka S, Matsumoto K, Hara H
Microbiology (Reading, England) 158 ( Pt 5 ) 1238 - 48 2012.5
Language：English Publishing type：Research paper (scientific journal) Publisher：Microbiology
The Rcs phosphorelay signal transduction system controls genes for capsule production and many other envelope-related functions and is implicated in biofilm formation. We investigated the activation of the Rcs system in a pgsA null mutant of Escherichia coli, which completely lacks the major acidic phospholipids phosphatidylglycerol and cardiolipin. We found that the Rcs activation, and consequent thermosensitivity, were suppressed by overexpression of the lgt gene, encoding diacylglyceryltransferase, which catalyses the modification of prolipoproteins that is the first step in the maturation and localization process of lipoproteins, and is a prerequisite for the later steps. The outer-membrane lipoprotein RcsF is an essential component of Rcs signalling. This lipoprotein was poorly localized to the outer membrane in the pgsA null mutant, probably because of the absence of phosphatidylglycerol, the major donor of diacylglycerol in the Lgt reaction. Even in a pgsA + background, the Rcs system was activated when RcsF was mislocalized to the inner membrane by alteration of the residues at positions 2 and 3 of its mature form to inner-membrane retention signals, or when it was mislocalized to the periplasm by fusing the mature form to maltose-binding protein. These results suggest that RcsF functions as a ligand for RcsC in activating Rcs signalling. Mislocalized versions of RcsF still responded to mutations pgsA, mdoH and tolB, further activating the Rcs system, although the rfaP mutation barely caused activation. It seems that RcsF must be localized in the outer membrane to respond effectively to stimuli from outside the cell. © 2012 SGM.
Presentations 【 display / non-display 】
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産学官連携リスクマネジメントの取り組み
長濵秀樹
宮崎大学産学・地域連携センター第２８回技術・研究発表交流会 2021.9.10
Event date： 2021.9.10
Language：Japanese Presentation type：Poster presentation
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研究成果有体物授受の管理について
長濵秀樹
宮崎大学産学・地域連携センター第２８回技術・研究発表交流会 2021.9.10
Event date： 2021.9.10
Language：Japanese Presentation type：Poster presentation
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産学官連携リスクマネジメントの取り組み
長濵秀樹
宮崎大学産学・地域連携センター第２７回技術・研究発表交流会 2020.9.17
Event date： 2020.9.17
Language：Japanese Presentation type：Poster presentation
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研究成果有体物授受の管理について
長濵秀樹
宮崎大学産学・地域連携センター第２７回技術・研究発表交流会 2020.9.17
Event date： 2020.9.17
Language：Japanese Presentation type：Poster presentation
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産学官連携リスクマネジメントモデルについて
狩野幹人,樋口人志,長濵秀樹
RA協議会第4回年次大会 (神戸国際会議場) リサーチ・アドミニストレーター協議会
Event date： 2018.9.19 - 2018.9.20
Language：Japanese Presentation type：Oral presentation (general)
Venue：神戸国際会議場
Industrial property rights 【 display / non-display 】
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微生物吸着剤およびこれを用いた微生物殺菌方法
木之下 広幸,三澤 尚明,安井 賢太郎,小林 太一,長濱 秀樹
Applicant：宮崎大学
Application no：2020-539594 Date applied：2020.12.21
Announcement no：WO2020/045580 Date announced：2020.3.5
Country of applicant：Domestic
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核酸吸着材
木之下 広幸,三澤 尚明,安井 賢太郎,小林 太一,長濱 秀樹
Applicant：宮崎大学
Application no：特願2020-033714 Date applied：2020.2.28
Announcement no：特開2021-133347 Date announced：2021.9.13
Country of applicant：Domestic
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微粒子吸着材及びその製造方法
木之下 広幸,三澤 尚明,安井 賢太郎,小林 太一,長濱 秀樹
Applicant：宮崎大学
Application no：特願2020-033708 Date applied：2020.2.28
Announcement no：特開2021-133344 Date announced：2021.9.13
Country of applicant：Domestic
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微生物吸着剤およびこれを用いた微生物殺菌方法
長濱 秀樹
Applicant：宮崎大学
Application no：PCT/JP2019/033956 Date applied：2019.8.29
Announcement no：WO/2020/045580 Date announced：2020.3.5
Country of applicant：Domestic
Awards 【 display / non-display 】
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Student Travel Grant
2005.7 IUMS（International Union of Microbiological Societies）
Hideki Nagahama