長濵 秀樹 (ナガハマ ヒデキ)

NAGAHAMA Hideki

写真a

所属

研究・産学地域連携推進機構

職名

助教

研究室電話番号

0985-58-7592

外部リンク

学位 【 表示 / 非表示

  • 博士(理学) ( 2007年3月   埼玉大学 )

研究分野 【 表示 / 非表示

  • その他 / その他  / 知的財産、技術移転

  • ライフサイエンス / 分子生物学  / 分子遺伝学

学外略歴 【 表示 / 非表示

  • 九州大学   有体物管理センター   学術共同研究者

    2019年4月 - 現在

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    国名:日本国

 

論文 【 表示 / 非表示

  • A quantitative protocol for DNA metabarcoding of springtails (Collembola). 査読あり

    Saitoh S, Aoyama H, Fujii S, Sunagawa H, Nagahama H, Akutsu M, Shinzato N, Kaneko N, Nakamori T

    Genome   59 ( 9 )   705 - 23   2016年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Genome  

    © 2016 Published by NRC Research Press. We developed a novel protocol with superior quantitative analysis results for DNA metabarcoding of Collembola, a major soil microarthropod order. Degenerate PCR primers were designed for conserved regions in the mitochondrial cytochrome c oxidase subunit I (mtCOI) and 16S ribosomal RNA (mt16S) genes based on published collembolan mitogenomes. The best primer pair was selected based on its ability to amplify each gene, irrespective of the species. DNA was extracted from 10 natural communities sampled in a temperate forest (with typically 25-30 collembolan species per 10 soil samples) and 10 mock communities (with seven cultured collembolan species). The two gene regions were then amplified using the selected primers, ligated with adapters for 454 technology, and sequenced. Examination of the natural community samples showed that 32 and 36 operational taxonomic units (defined at a 90% sequence similarity threshold) were recovered from the mtCOI and mt16S data, respectively, which were comparable to the results of the microscopic identification of 25 morphospecies. Further, sequence abundances for each collembolan species from the mtCOI and mt16S data of the mock communities, after normalization by using a species as the internal control, showed good correlation with the number of individuals in the samples (R = 0.91-0.99), although relative species abundances within a mock community sample estimated from sequences were skewed from community composition in terms of the number of individuals or biomass of the species. Thus, this protocol enables the comparison of collembolan communities in a quantitative manner by metabarcoding.

    DOI: 10.1139/gen-2015-0228

    Scopus

    PubMed

  • A rapid method of non-destructive DNA extraction from individual springtails (Collembola) 査読あり

    Aoyama H., Saitoh S., Fujii S., Nagahama H., Shinzato N., Kaneko N., Nakamori T.

    Applied Entomology and Zoology   50 ( 3 )   419 - 425   2015年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Applied Entomology and Zoology  

    © 2015, The Japanese Society of Applied Entomology and Zoology. In this study, we describe an easy and rapid method for non-destructive DNA extraction from a single Collembola individual, without dissection, lysis of the specimen, or purification of extracted DNA. We demonstrate that, after a single specimen has been heat-treated in Tris-EDTA (TE) buffer using a standard thermocycler, the solution can be used for PCR amplification of the mitochondrial cytochrome c oxidase subunit 1 (COI) gene region, typically used for DNA barcoding. With this method, the morphological features of Collembola commonly used for species identification are well preserved. This DNA extraction method is preferable for DNA barcoding where the sequencing and preservation of a large number of small and fragile specimens are required.

    DOI: 10.1007/s13355-015-0340-0

    Scopus

  • DNA metabarcoding of springtails (Collembola) 査読あり

    Saitoh, Seikoh; Aoyama, Hiroaki; Fujii, Saori; Sunagawa, Haruki; Nagahama, Hideki; Akutsu, Masako; Shinzato, Naoya; Kaneko, Nobuhiro; Nakamori, Taizo;

    GENOME   58 ( 5 )   274 - 274   2015年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Exploring the relationship between lipoprotein mislocalization and activation of the Rcs signal transduction system in Escherichia coli. 査読あり

    Shiba Y, Miyagawa H, Nagahama H, Matsumoto K, Kondo D, Matsuoka S, Matsumoto K, Hara H

    Microbiology (Reading, England)   158 ( Pt 5 )   1238 - 48   2012年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Microbiology  

    The Rcs phosphorelay signal transduction system controls genes for capsule production and many other envelope-related functions and is implicated in biofilm formation. We investigated the activation of the Rcs system in a pgsA null mutant of Escherichia coli, which completely lacks the major acidic phospholipids phosphatidylglycerol and cardiolipin. We found that the Rcs activation, and consequent thermosensitivity, were suppressed by overexpression of the lgt gene, encoding diacylglyceryltransferase, which catalyses the modification of prolipoproteins that is the first step in the maturation and localization process of lipoproteins, and is a prerequisite for the later steps. The outer-membrane lipoprotein RcsF is an essential component of Rcs signalling. This lipoprotein was poorly localized to the outer membrane in the pgsA null mutant, probably because of the absence of phosphatidylglycerol, the major donor of diacylglycerol in the Lgt reaction. Even in a pgsA + background, the Rcs system was activated when RcsF was mislocalized to the inner membrane by alteration of the residues at positions 2 and 3 of its mature form to inner-membrane retention signals, or when it was mislocalized to the periplasm by fusing the mature form to maltose-binding protein. These results suggest that RcsF functions as a ligand for RcsC in activating Rcs signalling. Mislocalized versions of RcsF still responded to mutations pgsA, mdoH and tolB, further activating the Rcs system, although the rfaP mutation barely caused activation. It seems that RcsF must be localized in the outer membrane to respond effectively to stimuli from outside the cell. © 2012 SGM.

    DOI: 10.1099/mic.0.056945-0

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    PubMed

  • Accumulation of sigmaS due to enhanced synthesis and decreased degradation in acidic phospholipid-deficient Escherichia coli cells. 査読あり

    Uchiyama J, Sasaki Y, Nagahama H, Itou A, Matsuoka S, Matsumoto K, Hara H

    FEMS microbiology letters   307 ( 2 )   120 - 7   2010年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:FEMS Microbiology Letters  

    The Escherichia coli pgsA3 mutation, which causes deficiency in acidic phospholipids, leads to a significant accumulation of σS. This accumulation is partly accounted for by the higher transcription level of rpoS; however, it has also been suggested that the cells accumulate σS post-transcriptionally. We found that the level of the small regulatory RNA RprA, which is involved in the promotion of rpoS translation, is higher in pgsA3 cells than in pgsA+ cells. Induction of altered rpoS mRNA that does not depend on RprA in pgsA+ cells did not increase the level of σS to the high level observed in pgsA3 cells, suggesting post-translational σS accumulation in the latter. The mRNA levels of clpX and clpP, whose products form a ClpXP protease that degrades σS, were much reduced in pgsA3 cells. Consistent with the reduced mRNA levels, the half-life of σS in pgsA3 cells was much longer than in pgsA+ cells, indicating that downregulation of the degradation is a major cause for the high σS content. We show that the downregulation can be partially attributed to activated CpxAR in the mutant cells, which causes repression of rpoE on whose gene product the expression of clpPX depends. © 2010 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.

    DOI: 10.1111/j.1574-6968.2010.01964.x

    Scopus

    PubMed

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講演・口頭発表等 【 表示 / 非表示

  • 産学官連携リスクマネジメントの取り組み

    長濵秀樹

    宮崎大学産学・地域連携センター第28回技術・研究発表交流会  2021年9月10日 

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    開催年月日: 2021年9月10日

    記述言語:日本語   会議種別:ポスター発表  

  • 研究成果有体物授受の管理について

    長濵秀樹

    宮崎大学産学・地域連携センター第28回技術・研究発表交流会  2021年9月10日 

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    開催年月日: 2021年9月10日

    記述言語:日本語   会議種別:ポスター発表  

  • 産学官連携リスクマネジメントの取り組み

    長濵秀樹

    宮崎大学産学・地域連携センター第27回技術・研究発表交流会  2020年9月17日 

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    開催年月日: 2020年9月17日

    記述言語:日本語   会議種別:ポスター発表  

  • 研究成果有体物授受の管理について

    長濵秀樹

    宮崎大学産学・地域連携センター第27回技術・研究発表交流会  2020年9月17日 

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    開催年月日: 2020年9月17日

    記述言語:日本語   会議種別:ポスター発表  

  • 産学官連携リスクマネジメントモデルについて

    狩野幹人,樋口人志,長濵秀樹

    RA協議会第4回年次大会  (神戸国際会議場)  リサーチ・アドミニストレーター協議会

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    開催年月日: 2018年9月19日 - 2018年9月20日

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:神戸国際会議場  

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産業財産権 【 表示 / 非表示

  • 微生物吸着剤およびこれを用いた微生物殺菌方法

    長濱 秀樹

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    出願番号:2020-539594  出願日:2020年12月21日

    公開番号:WO2020/045580  公開日:2020年3月5日

    出願国:国内  

  • 核酸吸着材

    核酸吸着材

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    出願番号:特願2020-033714  出願日:2020年2月28日

    公開番号:特開2021-133347  公開日:2021年9月13日

    出願国:国内  

  • 微粒子吸着材及びその製造方法

    微粒子吸着材及びその製造方法

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    出願番号:特願2020-033708  出願日:2020年2月28日

    公開番号:特開2021-133344  公開日:2021年9月13日

    出願国:国内  

受賞 【 表示 / 非表示

  • Student Travel Grant

    2005年7月   IUMS(International Union of Microbiological Societies)  

    Hideki Nagahama

その他競争的資金獲得実績 【 表示 / 非表示

  • 沖縄に固有な微生物資源の利用拡大に向けた休眠覚醒因子の利用研究

    2013年04月 - 2014年03月

    公益財団法人琉球大学後援財団  公益財団法人琉球大学後援財団 教育研究奨励事業 

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    資金種別:競争的資金