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Affiliation |
Faculty of Regional Innovation |
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External Link |
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HASHIGUCHI Takuyu
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Research Areas 【 display / non-display 】
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Life Science / Bioorganic chemistry
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Life Science / Food sciences
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Environmental Science/Agriculture Science / Science in plant genetics and breeding
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Life Science / Applied biochemistry
Education 【 display / non-display 】
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University of Miyazaki Graduate School, Division of Agricultural Science
2011.4 - 2014.3
Country：Japan
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University of Miyazaki Graduate School, Division of Agriculture
2009.4 - 2011.3
Country：Japan
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University of Miyazaki Faculty of Agriculture
2005.4 - 2009.3
Country：Japan
Campus Career 【 display / non-display 】
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University of Miyazaki Faculty of Regional Innovation
2022.07 - Now
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University of Miyazaki Faculty of Regional Innovation Researcher
2022.04 - 2022.06
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University of Miyazaki Faculty of Agriculture Department of Animal and Grassland Sciences Researcher
2018.04 - 2022.03
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University of Miyazaki Faculty of Agriculture Department of Biochemistry and Applied Biosciences Researcher
2014.04 - 2015.03
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University of Miyazaki Interdisciplinary Graduate school of Agriculture and Engineering Applied Biological Sciences Special researcher of the Japan Society for the Promotion of Science
2012.04 - 2014.03
External Career 【 display / non-display 】
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宮崎県総合農業試験場 研究員
2017.11 - 2018.3
Country：Japan
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エディットフォース株式会社 主任研究員
2017.2 - 2017.10
Country：Japan
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National Institute of Environmental Health Sciences (NIEHS/NIH) Visiting Fellow
2015.4 - 2017.1
Country：Japan
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National Institute of Environmental Health Sciences (NIEHS/NIH) Reproductive and Developmental Biology Laboratory Special Volunteer
2014.4 - 2015.3
Country：Japan
Qualification acquired 【 display / non-display 】
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Chief Person of Radiation Handling （first and second kind）
Papers 【 display / non-display 】
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Biochemical changes in recombinant inbred lines of soybean (Glycine max L. Merr.) under storage-related stresses Reviewed
Pakanan Jiamtae*, Takuyu Hashiguchi*, Sorawich Pongpiyapaiboon, Intouch Lelapiyamit, Hidenori Tanaka, Masatsugu Hashiguchi, Ryo Akashi, *Equally contributed
Journal of Crop Science and Biotechnology 2023
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
DOI： 10.1007/s12892-023-00192-6
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Construction of prediction models for growth traits of soybean cultivars based on phenotyping in diverse genotype and environment combinations. Reviewed
Manggabarani AM*, Hashiguchi T*, Hashiguchi M, Hayashi A, Kikuchi M, Mustamin Y, Bamba M, Kodama K, Tanabata T, Isobe S, Tanaka H, Akashi R, Nakaya A, Sato S, *Equally contributed.
DNA research 2022.8
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
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Tanabata Takanari, Kodama Kunihiro, Hashiguchi Takuyu, Inomata Daisuke, Tanaka Hidenori, Isobe Sachiko
Breeding Science 72 ( 1 ) 85 - 95 2022
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Japanese Society of Breeding
<p>Plant phenotyping technology has been actively developed in recent years, but the introduction of these technologies into the field of agronomic research has not progressed as expected, in part due to the need for flexibility and low cost. "DIY" (Do It Yourself) methodologies are an efficient way to overcome such obstacles. Devices with modular functionality are critical to DIY experimentation, allowing researchers flexibility of design. In this study, we developed a plant conveyance system using a commercial AGV as a case study of DIY plant phenotyping. The convey module consists of two devices, a running device and a plant-handling device. The running device was developed based on a commercial AGV (Automated Guided Vehicle) Kit. The plant-handling device, plant stands, and pot attachments were originally designed and fabricated by us and our associates. Software was also developed for connecting the devices and operating the system. The run route was set with magnetic tape, which can be easily changed or rerouted. Our plant delivery system was developed with low cost and having high flexibility, as a unit that can contribute to others' DIY' plant research efforts as well as our own. It is expected that the developed devices will contribute to diverse phenotype observations of plants in the greenhouse as well as to other important functions in plant breeding and agricultural production.</p>
DOI： 10.1270/jsbbs.21070
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Varietal differences in flavonoid and antioxidant activity in Japanese soybean accessions Reviewed
Arifin H.A.*, Hashiguchi T.*, Nagahama K., Hashiguchi M., Muguerza M., Sakakibara Y., Tanaka H., Akashi R. *Equally contributed.
Bioscience, biotechnology, and biochemistry 85 ( 4 ) 916 - 922 2021.3
Language：English Publishing type：Research paper (scientific journal) Publisher：Bioscience, biotechnology, and biochemistry
Soybean seeds contain many antioxidants, including flavonoids and other phytochemicals. Isoflavone is a phytoestrogen that mimics estrogenic effects on target tissues and also exerts antioxidant activity by sequestering free radicals. Despite many cultivars developed to date, varietal differences in flavonoid content and antioxidant activity in Japanese soybean accessions remain less well characterized. Here, we evaluated the seed content of isoflavones, total flavonoids, and total phenolics in 26 soybean accessions. Next, the antioxidant activity of each accession was examined using antioxidant responsive element (ARE) linked to a luciferase reporter in human HepG2 stable cells. The relative ARE luciferase activity rate of all soybean accessions varied up to 4-fold which ranged from 1.00 to 4.02; and 22 accessions exhibited significant antioxidant activities. Correlation analysis indicated that the level of total isoflavone moderately correlated with antioxidant activity.
DOI： 10.1093/bbb/zbaa104
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Quantitative analysis of seven plant hormones in Lotus japonicus using standard addition method Reviewed
Hashiguchi, T, Hashiguchi, M, Tanaka, H, Fukushima, K, Gondo, T, Akashi, R
PLoS One 16 ( 2 ) e0247276 2021.2
Language：English Publishing type：Research paper (scientific journal)
MISC 【 display / non-display 】
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リン酸化を介した核内受容体研究の新展開 Invited
橋口拓勇, 根岸正彦
バイオサイエンスとインダストリー (B&I) Submitted 2023
Authorship：Lead author Publishing type：Rapid communication, short report, research note, etc. (scientific journal)
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環境汚染とヘモグロビン～植物による組換えヘモグロビン研究の最前線～ Invited
Takuyu Hashiguchi
Farumashia 58 ( 6 ) 608 - 608 2022.6
Authorship：Lead author Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (scientific journal) Publisher：The Pharmaceutical Society of Japan
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Recent progresses on the phylogenetic analysis and the chemical profiling of wild soybeans (Glycine soja): An attempt for smart breeding. Invited
Japan Association for Techno-innovation in Agriculture, Forestry and Fisheries Journal Submitted 2022
Authorship：Lead author Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (bulletin of university, research institution)
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NBRPミヤコグサ・ダイズにおけるバイオリソース整備 Invited
橋口 正嗣，田中 秀典，橋口 拓勇，明石 良
農業および園芸 96 ( 2 ) 153 - 162 2021.2
Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (scientific journal)
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ヒト cytosolic sulfotransferase発現大腸菌を用いた硫酸化ポリフェノール類の部位選択的合成とその応用
下平武彦, 黒木勝久, 橋口拓勇, 水光正仁, 榊原陽一
硫酸と工業 73 ( 11 ) 127 - 133 2020.11
Publishing type：Article, review, commentary, editorial, etc. (scientific journal)
Presentations 【 display / non-display 】
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シバ属における新たな形態計測法の検討 – 全周囲画像による3Dモデルの構築と活用–
Sorawich Pongpiyapaiboon, 田中 秀典, 橋口 拓勇, 橋口 正嗣, 林 篤司, 七夕 高也, 兒玉 晋洋, 磯部 祥子, 明石 良
日本育種学会第141回講演会 2022.3.20
Event date： 2022.3.20 - 2022.3.22
Language：English Presentation type：Oral presentation (general)
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安定同位体を用いない標準添加法による植物ホルモンの定量
福島公貴, 橋口拓勇, 橋口正嗣, 明石良
植物微生物研究会 2021.9.9
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蛍光灯およびLED照明下におけるミヤコグサ実験系統の生育特性
橋口正嗣, 橋口拓勇, 田中秀典, 明石良
植物微生物研究会 2021.9.9
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標準添加法を用いたミヤコグサにおける植物ホルモン定量法の確立
橋口 拓勇, 福島 公貴, 橋口 正嗣, 田中 秀典, 権藤 崇裕, 明石 良
日本農芸化学会 2021.3
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ダイズおよびツルマメのプロテオーム解析
橋口 拓勇,橋口 正嗣,田中 秀典,権藤 崇裕,明石 良
日本農芸化学会 2020年度大会 (福岡)
Event date： 2020.3.25 - 2020.3.28
Language：Japanese Presentation type：Oral presentation (general)
Venue：福岡
Awards 【 display / non-display 】
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Featured home page article
2016.9 Molecular Endocrinology
Hashiguchi, T., Arakawa, S., Takahashi, S., Gonzalez J.F., Sueyoshi, T., Negishi, M.
Award type：Honored in official journal of a scientific society, scientific journal Country：Japan
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優秀ポスター賞
2013.6 P450, UGT, SULT研究会 (現:内外環境応答・代謝酵素研究会)
橋口拓勇
Award type：Award from Japanese society, conference, symposium, etc. Country：Japan
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Young Investigator Award
2011.8 日本フードファクター学会(JSoFF)
橋口拓勇
Award type：Award from Japanese society, conference, symposium, etc. Country：Japan
Grant-in-Aid for Scientific Research 【 display / non-display 】
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植物ー微生物間の情報伝達分子としてのフラボノイド硫酸体に関する研究
Grant number：23K05027 2023.04 - 2026.03
独立行政法人日本学術振興会 科学研究費基金 基盤研究（C）
Authorship：Principal investigator
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翻訳後修飾としてのチロシン硫酸化の空間および時間的強制機構の理解と機能解明
Grant number：22H02262 2022.04 - 2026.03
独立行政法人日本学術振興会 科学研究費補助金 基盤研究(B)
榊原 陽一、
Authorship：Coinvestigator(s)
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植物における硫酸転移酵素の生理機能に関する研究
Grant number：12J01404 2012.04 - 2014.03
科学研究費補助金 特別研究員奨励費
Authorship：Principal investigator
Other research activities 【 display / non-display 】
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日本分子生物学会
2019.12
ミヤコグサ・ダイズバイオリソース(NBRP)の展示
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日本プロテオーム学会
2019.07
ミヤコグサ・ダイズバイオリソース(NBRP)の展示
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日本農芸化学会
2019.03
ミヤコグサ・ダイズバイオリソース(NBRP)の展示
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日本分子生物学会
2018.11
ミヤコグサ・ダイズバイオリソース(NBRP)の展示
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日本植物学会
2018.09
ミヤコグサ・ダイズバイオリソース(NBRP)の展示