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Affiliation |
Faculty of Regional Innovation |
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External Link |
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HASHIGUCHI Takuyu
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Research Areas 【 display / non-display 】
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Life Science / Bioorganic chemistry
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Life Science / Food sciences
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Environmental Science/Agriculture Science / Science in plant genetics and breeding
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Life Science / Applied biochemistry
Education 【 display / non-display 】
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University of Miyazaki Graduate School, Division of Agricultural Science
2011.4 - 2014.3
Country：Japan
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University of Miyazaki Graduate School, Division of Agriculture
2009.4 - 2011.3
Country：Japan
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University of Miyazaki Faculty of Agriculture
2005.4 - 2009.3
Country：Japan
Campus Career 【 display / non-display 】
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University of Miyazaki Faculty of Regional Innovation
2022.07 - Now
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University of Miyazaki Faculty of Regional Innovation Researcher
2022.04 - 2022.06
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University of Miyazaki Faculty of Agriculture Department of Animal and Grassland Sciences Researcher
2018.04 - 2022.03
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University of Miyazaki Faculty of Agriculture Department of Biochemistry and Applied Biosciences Researcher
2014.04 - 2015.03
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University of Miyazaki Interdisciplinary Graduate school of Agriculture and Engineering Applied Biological Sciences Special researcher of the Japan Society for the Promotion of Science
2012.04 - 2014.03
External Career 【 display / non-display 】
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宮崎県総合農業試験場 研究員
2017.11 - 2018.3
Country：Japan
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エディットフォース株式会社 主任研究員
2017.2 - 2017.10
Country：Japan
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National Institute of Environmental Health Sciences (NIEHS/NIH) Visiting Fellow
2015.4 - 2017.1
Country：Japan
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National Institute of Environmental Health Sciences (NIEHS/NIH) Reproductive and Developmental Biology Laboratory Special Volunteer
2014.4 - 2015.3
Country：Japan
Professional Memberships 【 display / non-display 】
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日本育種学会
2019.6 - 2022.1
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内外環境応答・代謝酵素研究会(旧P450,UGT,SULT研究会)
2013.6
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日本フードファクター学会
2010.6 - 2013.12
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日本農芸化学会
2009.12
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日本プロテオーム学会
2009.12 - 2014.1
Qualification acquired 【 display / non-display 】
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Chief Person of Radiation Handling （first and second kind）
Papers 【 display / non-display 】
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Kurogi K., Sakakibara Y., Hashiguchi T., Kakuta Y., Kanekiyo M., Teramoto T., Fukushima T., Bamba T., Matsumoto J., Fukusaki E., Kataoka H., Suiko M.
PNAS Nexus 3 ( 3 ) pgae097 2024.3
Language：English Publishing type：Research paper (scientific journal) Publisher：PNAS Nexus
Cytosolic sulfotransferases (SULTs) are cytosolic enzymes that catalyze the transfer of sulfonate group to key endogenous compounds, altering the physiological functions of their substrates. SULT enzymes catalyze the O-sulfonation of hydroxy groups or N-sulfonation of amino groups of substrate compounds. In this study, we report the discovery of C-sulfonation of α,β-unsaturated carbonyl groups mediated by a new SULT enzyme, SULT7A1, and human SULT1C4. Enzymatic assays revealed that SULT7A1 is capable of transferring the sulfonate group from 3′-phosphoadenosine 5′-phosphosulfate to the α-carbon of α,β-unsaturated carbonyl-containing compounds, including cyclopentenone prostaglandins as representative endogenous substrates. Structural analyses of SULT7A1 suggest that the C-sulfonation reaction is catalyzed by a novel mechanism mediated by His and Cys residues in the active site. Ligand-activity assays demonstrated that sulfonated 15-deoxy prostaglandin J2 exhibits antagonist activity against the prostaglandin receptor EP2 and the prostacyclin receptor IP. Modification of α,β-unsaturated carbonyl groups via the new prostaglandin-sulfonating enzyme, SULT7A1, may regulate the physiological function of prostaglandins in the gut. Discovery of C-sulfonation of α,β-unsaturated carbonyl groups will broaden the spectrum of potential substrates and physiological functions of SULTs.
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Development of a digital phenotyping system using 3D model reconstruction for zoysiagrass Reviewed
Pongpiyapaiboon S., Tanaka H., Hashiguchi M., Hashiguchi T., Hayashi A., Tanabata T., Isobe S., Akashi R.
Plant Phenome Journal 6 ( 1 ) 2023.12
Publishing type：Research paper (scientific journal) Publisher：Plant Phenome Journal
Digital phenotyping, particularly the use of plant 3D models, is a promising method for high-throughput plant evaluation. Although many recent studies on the topic have been published, further research is needed to apply it to breeding research and other related fields. In this study, using a 3D model phenotyping system we developed, we reconstructed and analyzed 20 accessions of zoysiagrass (Zoysia spp.), including three species and their hybrid, over a period of 1 year. Artificial neural network with three hidden layers was able to effectively remove nonplant parts while retaining plant parts that were incorrectly removed using the cropping method, offering a robust and flexible approach for post-processing of 3D models. The system also demonstrated its ability to accurately evaluate a range of traits, including height, area, and color using red green blue (RGB)-based vegetation indices. The results showed a high correlation between the estimated volume obtained from voxel 3D model and dry weight, enabling its use as a non-destructive method for measuring plant volume. In addition, we found that the green red normalized difference index from RGB-based indices was similar to the commonly used normalized difference vegetation index in controlled illumination conditions. These results demonstrate the potential for three-dimensional model phenotyping to facilitate plant breeding, particularly in the field of turfgrass and feed crops.
DOI： 10.1002/ppj2.20076
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Pakanan Jiamtae*, Takuyu Hashiguchi*, Sorawich Pongpiyapaiboon, Intouch Lelapiyamit, Hidenori Tanaka, Masatsugu Hashiguchi, Ryo Akashi, *Equally contributed
Journal of Crop Science and Biotechnology 26 ( 4 ) 467 - 488 2023
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
DOI： 10.1007/s12892-023-00192-6
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Construction of prediction models for growth traits of soybean cultivars based on phenotyping in diverse genotype and environment combinations. Reviewed
Manggabarani AM*, Hashiguchi T*, Hashiguchi M, Hayashi A, Kikuchi M, Mustamin Y, Bamba M, Kodama K, Tanabata T, Isobe S, Tanaka H, Akashi R, Nakaya A, Sato S, *Equally contributed.
DNA research 2022.8
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
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Tanabata Takanari, Kodama Kunihiro, Hashiguchi Takuyu, Inomata Daisuke, Tanaka Hidenori, Isobe Sachiko
Breeding Science 72 ( 1 ) 85 - 95 2022
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Japanese Society of Breeding
<p>Plant phenotyping technology has been actively developed in recent years, but the introduction of these technologies into the field of agronomic research has not progressed as expected, in part due to the need for flexibility and low cost. "DIY" (Do It Yourself) methodologies are an efficient way to overcome such obstacles. Devices with modular functionality are critical to DIY experimentation, allowing researchers flexibility of design. In this study, we developed a plant conveyance system using a commercial AGV as a case study of DIY plant phenotyping. The convey module consists of two devices, a running device and a plant-handling device. The running device was developed based on a commercial AGV (Automated Guided Vehicle) Kit. The plant-handling device, plant stands, and pot attachments were originally designed and fabricated by us and our associates. Software was also developed for connecting the devices and operating the system. The run route was set with magnetic tape, which can be easily changed or rerouted. Our plant delivery system was developed with low cost and having high flexibility, as a unit that can contribute to others' DIY' plant research efforts as well as our own. It is expected that the developed devices will contribute to diverse phenotype observations of plants in the greenhouse as well as to other important functions in plant breeding and agricultural production.</p>
DOI： 10.1270/jsbbs.21070
MISC 【 display / non-display 】
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核内受容体のリン酸化を介した遺伝子発現制御機構 Invited
橋口拓勇, 根岸正彦
バイオサイエンスとインダストリー (B&I) Submitted 2023.1
Authorship：Lead author Publishing type：Rapid communication, short report, research note, etc. (scientific journal)
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環境汚染とヘモグロビン～植物による組換えヘモグロビン研究の最前線～ Invited
Takuyu Hashiguchi
Farumashia 58 ( 6 ) 608 - 608 2022.6
Authorship：Lead author Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (scientific journal) Publisher：The Pharmaceutical Society of Japan
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Recent progresses on the phylogenetic analysis and the chemical profiling of wild soybeans (Glycine soja): An attempt for smart breeding. Invited
Japan Association for Techno-innovation in Agriculture, Forestry and Fisheries Journal Submitted 2022
Authorship：Lead author Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (bulletin of university, research institution)
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NBRPミヤコグサ・ダイズにおけるバイオリソース整備 Invited
橋口 正嗣，田中 秀典，橋口 拓勇，明石 良
農業および園芸 96 ( 2 ) 153 - 162 2021.2
Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (scientific journal)
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ヒト cytosolic sulfotransferase発現大腸菌を用いた硫酸化ポリフェノール類の部位選択的合成とその応用
下平武彦, 黒木勝久, 橋口拓勇, 水光正仁, 榊原陽一
硫酸と工業 73 ( 11 ) 127 - 133 2020.11
Publishing type：Article, review, commentary, editorial, etc. (scientific journal)
Presentations 【 display / non-display 】
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シバ属における新たな形態計測法の検討 – 全周囲画像による3Dモデルの構築と活用–
Sorawich Pongpiyapaiboon, 田中 秀典, 橋口 拓勇, 橋口 正嗣, 林 篤司, 七夕 高也, 兒玉 晋洋, 磯部 祥子, 明石 良
日本育種学会第141回講演会 2022.3.20
Event date： 2022.3.20 - 2022.3.22
Language：English Presentation type：Oral presentation (general)
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安定同位体を用いない標準添加法による植物ホルモンの定量
福島公貴, 橋口拓勇, 橋口正嗣, 明石良
植物微生物研究会 2021.9.9
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蛍光灯およびLED照明下におけるミヤコグサ実験系統の生育特性
橋口正嗣, 橋口拓勇, 田中秀典, 明石良
植物微生物研究会 2021.9.9
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圃場栽培したミヤコグサの表現型を制御するゲノム領域の解析: 画像解析 を利用したアプローチ
高橋 隼, 番場 大, 三岡, 周子,Yusdar, Mustamin,Madiha, Manggabarani, 磯村 芳, 子,林 篤司, 七夕 高也, 橋口 拓勇, 橋口 正嗣, 藤 修正
日本植物学会
Event date： 2021.9
Presentation type：Oral presentation (general)
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標準添加法を用いたミヤコグサにおける植物ホルモン定量法の確立
橋口 拓勇, 福島 公貴, 橋口 正嗣, 田中 秀典, 権藤 崇裕, 明石 良
日本農芸化学会 2021.3
Awards 【 display / non-display 】
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卓越研究員候補者
2021.10 日本学術振興会
橋口拓勇
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Featured home page article
2016.9 Molecular Endocrinology
Hashiguchi, T., Arakawa, S., Takahashi, S., Gonzalez J.F., Sueyoshi, T., Negishi, M.
Award type：Honored in official journal of a scientific society, scientific journal Country：Japan
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優秀ポスター賞
2013.6 P450, UGT, SULT研究会 (現:内外環境応答・代謝酵素研究会)
橋口拓勇
Award type：Award from Japanese society, conference, symposium, etc. Country：Japan
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Young Investigator Award
2011.8 日本フードファクター学会(JSoFF)
橋口拓勇
Award type：Award from Japanese society, conference, symposium, etc. Country：Japan
Grant-in-Aid for Scientific Research 【 display / non-display 】
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植物ー微生物間の情報伝達分子としてのフラボノイド硫酸体に関する研究
Grant number：23K05027 2023.04 - 2026.03
独立行政法人日本学術振興会 科学研究費基金 基盤研究(C)
Authorship：Principal investigator
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翻訳後修飾としてのチロシン硫酸化の空間および時間的強制機構の理解と機能解明
Grant number：22H02262 2022.04 - 2026.03
独立行政法人日本学術振興会 科学研究費補助金 基盤研究(B)
榊原 陽一、
Authorship：Coinvestigator(s)
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植物における硫酸転移酵素の生理機能に関する研究
Grant number：12J01404 2012.04 - 2014.03
科学研究費補助金 特別研究員奨励費
Authorship：Principal investigator
Other research activities 【 display / non-display 】
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日本農芸化学会2024年度大会
2024.03
ミヤコグサ・ダイズバイオリソース(NBRP)の展示
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第46回日本分子生物学会
2023.12
ミヤコグサ・ダイズバイオリソース(NBRP)の展示
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日本植物生理学会
2023.03
ミヤコグサ・ダイズバイオリソース(NBRP)の展示
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第45回日本分子生物学会
2022.11
ミヤコグサ・ダイズバイオリソース(NBRP)の展示
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日本植物生理学会
2022.03
ミヤコグサ・ダイズバイオリソース(NBRP)の展示
Committee Memberships 【 display / non-display 】
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日本フードファクタ―学会・日本農芸化学会西日本支部会合同大会 実行委員
2020.11
Committee type：学協会
西日本支部大会開催予定
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日本プロテオーム学会 実行委員
2019.7
Committee type：学協会