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Affiliation |
Faculty of Medicine College Hospital Orthopedics department |
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Title |
Assistant Professor |
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External Link |
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OHTA Tomomi
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Papers 【 display / non-display 】
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Tmem161a regulates bone formation and bone strength through the P38 MAPK pathway Reviewed
Nagai T., Sekimoto T., Kurogi S., Ohta T., Miyazaki S., Yamaguchi Y., Tajima T., Chosa E., Imasaka M., Yoshinobu K., Araki K., Araki M., Choijookhuu N., Sato K., Hishikawa Y., Funamoto T.
Scientific Reports 13 ( 1 ) 14639 2023.12
Language：English Publishing type：Research paper (scientific journal) Publisher：Scientific Reports
Bone remodeling is an extraordinarily complex process involving a variety of factors, such as genetic, metabolic, and environmental components. Although genetic factors play a particularly important role, many have not been identified. In this study, we investigated the role of transmembrane 161a (Tmem161a) in bone structure and function using wild-type (WT) and Tmem161a-depleted (Tmem161aGT/GT) mice. Mice femurs were examined by histological, morphological, and bone strength analyses. Osteoblast differentiation and mineral deposition were examined in Tmem161a-overexpressed, -knockdown and -knockout MC3T3-e1 cells. In WT mice, Tmem161a was expressed in osteoblasts of femurs; however, it was depleted in Tmem161aGT/GT mice. Cortical bone mineral density, thickness, and bone strength were significantly increased in Tmem161aGT/GT mice femurs. In MC3T3-e1 cells, decreased expression of alkaline phosphatase (ALP) and Osterix were found in Tmem161a overexpression, and these findings were reversed in Tmem161a-knockdown or -knockout cells. Microarray and western blot analyses revealed upregulation of the P38 MAPK pathway in Tmem161a-knockout cells, which referred as stress-activated protein kinases. ALP and flow cytometry analyses revealed that Tmem161a-knockout cells were resistant to oxidative stress. In summary, Tmem161a is an important regulator of P38 MAPK signaling, and depletion of Tmem161a induces thicker and stronger bones in mice.
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第2趾DIP関節脱臼に対する観血的整復術後の早期スポーツ復帰の一例
川越 亮, 田島 卓也, 山口 奈美, 大田 智美, 長澤 誠, 森田 雄大, 横江 琢示, 帖佐 悦男
整形外科と災害外科 72 ( 3 ) 485 - 487 2023.9
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：西日本整形・災害外科学会
【はじめに】観血的整復術を必要とした第2趾のDIP関節脱臼の1例を経験した．【症例】16歳男性．空手中に受傷．第2趾DIP関節脱臼で徒手整復を行ったが整復不能であったため，観血的整復術を行った．整復阻害因子は長趾屈筋腱であり，蹠側板の嵌頓は認めなかった．術後早期の競技復帰希望があったため母趾とのbuddy tapingを指示し復帰とした．術後6か月が経過し特に問題なく競技継続している．【考察】DIP関節脱臼の整復阻害因子は蹠側板や長趾屈筋腱の報告があり，本症例では長趾屈筋腱であった．また，術後のスポーツ復帰について，競技によっては症状次第で早期復帰が見込めると考えられた．
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A case of anterior translation of the radial head after Sauvé-Kapandji procedure Reviewed
Kai Itono, Ota Tomomi, Chosa Etsuo
Journal of Japan Elbow Society 30 ( 2 ) 392 - 397 2023
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Japan Elbow Society
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足部びまん性色素性絨毛結節性滑膜炎に対し，関節鏡下腫瘍切除術を施行した一例 Reviewed
外山 宗樹, 横江 琢示, 田島 卓也, 山口 奈美, 大田 智美, 長澤 誠, 森田 雄大, 川越 秀一, 帖佐 悦男
整形外科と災害外科 71 ( 4 ) 725 - 729 2022.9
Language：Japanese Publishing type：Case report Publisher：西日本整形・災害外科学会
【背景】色素性絨毛結節性滑膜炎（Pigmented villonodular Synovitis, PVS）は関節滑膜や腱鞘に発生する比較的稀な良性腫瘍である．多くは膝関節周囲に発生し，足関節発生症例は稀である．今回，足部びまん性PVSに対して関節鏡下腫瘍切除術を行い，良好な短期成績を得た一例を経験したので報告する．【症例】23歳女性．数年前から左足部外側に腫瘤を自覚していたが，放置していた．しかしながら，腫瘤の消退を認めず前医を受診し，PVSの疑いで当科紹介となった．当科では関節鏡下腫瘍切除術を施行した．足関節前方および後足部鏡視下併用で足関節，足関節周囲，距骨下に病変を認め，関節鏡視下に腫瘍を切除した．術後病理診断ではびまん性PVSの診断であった．術後26ヶ月経過し，再発なく経過している．【結語】足部びまん性PVSに対し，足関節前方および後足部鏡視下併用での関節鏡下腫瘍切除術を施行し，良好な短期成績を得た一例を経験した．
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Miyazaki Shihoko, Funamoto Taro, Sekimoto Tomohisa, Kurogi Syuji, Ohta Tomomi, Nagai Takuya, Tajima Takuya, Imasaka Mai, Yoshinobu Kumiko, Araki Kimi, Araki Masatake, Choijookhuu Narantsog, Hishikawa Yoshitaka, Chosa Etsuo
ACTA HISTOCHEMICA ET CYTOCHEMICA 55 ( 3 ) 99 - 110 2022
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY
Epithelial protein lost in neoplasm (EPLIN) is an actin-associated cytoskeletal protein that plays an important role in epithelial cell adhesion. EPLIN has two isoforms: EPLINα and EPLINβ. In this study, we investigated the role of EPLINβ in osteoblasts using EPLINβ-deficient (<i>EPLINβ<sup>GT/GT</sup></i>) mice. The skeletal phenotype of <i>EPLINβ<sup>GT/GT</sup></i> mice is indistinguishable from the wildtype (WT), but bone properties and strength were significantly decreased compared with WT littermates. Histomorphological analysis revealed altered organization of bone spicules and osteoblast cell arrangement, and decreased alkaline phosphatase activity in <i>EPLINβ<sup>GT/GT</sup></i> mouse bones. Transmission electron microscopy revealed wider intercellular spaces between osteoblasts in <i>EPLINβ<sup>GT/GT</sup></i> mice, suggesting aberrant cell adhesion. In <i>EPLINβ<sup>GT/GT</sup></i> osteoblasts, α- and β-catenins and F-actin were observed at the cell membrane, but OB-cadherin was localized at the perinuclear region, indicating that cadherin-catenin complexes were not formed. EPLINβ knockdown in MC3T3-e1 osteoblast cells showed similar results as in calvaria cell cultures. Bone formation markers, such as <i>RUNX2</i>, <i>Osterix</i>, <i>ALP</i>, and <i>Col1a1</i> mRNA were reduced in EPLINβ knockdown cells, suggesting an important role for EPLINβ in osteoblast formation. In conclusion, we propose that EPLINβ is involved in the assembly of cadherin-catenin complexes in osteoblasts and affects bone formation.
DOI： 10.1267/ahc.22-00027
Presentations 【 display / non-display 】
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足関節に生じた色素性絨毛結節性滑膜炎の一例
外山 宗樹, 横江 琢示, 田島 卓也, 山口 奈美, 泉 俊彦, 大田 智美, 長澤 誠, 森田 雄大, 川越 秀一, 帖佐 悦男
第142回西日本整形・災害外科学会学術集会
Event date： 2021.12.11 - 2021.12.12
Presentation type：Oral presentation (general)
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上肢に発生した非結核性抗酸菌症－難治例の検討－
喜多 恒允, 大田 智美, 田島 卓也, 山口 奈美, 泉 俊彦, 長澤 誠, 森田 雄大, 横江 琢示, 川越 秀一, 森田 恭史, 帖佐 悦男
第142回西日本整形・災害外科学会学術集会
Event date： 2021.12.11 - 2021.12.12
Presentation type：Oral presentation (general)
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足関節に生じた色素性絨毛結節性滑膜炎の一例
外山 宗樹, 横江 琢示, 田島 卓也, 山口 奈美, 泉 俊彦, 大田 智美, 長澤 誠, 森田 雄大, 川越 秀一, 帖佐 悦男
第142回西日本整形・災害外科学会学術集会
Event date： 2021.12.11 - 2021.12.12
Presentation type：Oral presentation (general)
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上肢に発生した非結核性抗酸菌症－難治例の検討－
喜多 恒允, 大田 智美, 田島 卓也, 山口 奈美, 泉 俊彦, 長澤 誠, 森田 雄大, 横江 琢示, 川越 秀一, 森田 恭史, 帖佐 悦男
第142回西日本整形・災害外科学会学術集会
Event date： 2021.12.11 - 2021.12.12
Presentation type：Oral presentation (general)
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化膿性肩関節炎後にリバース型人工肩関節全置換術を施工した1例
泉 俊彦, 長澤 誠, 大田 智美, 川越 秀一, 帖佐 悦男
第48回日本肩関節学会学術集会
Event date： 2021.10.29 - 2021.10.30
Presentation type：Oral presentation (general)