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Frontier Science Research Center Department of Promotion of advanced Research Division of Young & Excellent Researcher Development |
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Associate Professor |
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SAITO Akatsuki
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Research Interests 【 display / non-display 】
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応用獣医学
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宿主因子
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動物モデル
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フラビウイルス
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エイズ
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ウイルス
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霊長類
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獣医微生物学
Research Areas 【 display / non-display 】
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Life Science / Veterinary medical science
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Life Science / Laboratory animal science
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Life Science / Molecular biology
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Life Science / Immunology
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Life Science / Virology
Education 【 display / non-display 】
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The University of Tokyo
- 2011.3
Country：Japan
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University of Miyazaki Faculty of Agriculture
- 2008.3
Country：Japan
Professional Memberships 【 display / non-display 】
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日本エイズ学会
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日本獣医学会
Papers 【 display / non-display 】
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Enhanced fusogenicity and pathogenicity of SARS-CoV-2 Delta P681R mutation Reviewed
Akatsuki Saito, Takashi Irie, Rigel Suzuki, Tadashi Maemura, Hesham Nasser, Keiya Uriu, Yusuke Kosugi, Kotaro Shirakawa, Kenji Sadamasu, Izumi Kimura, Jumpei Ito, Jiaqi Wu, Kiyoko Iwatsuki-Horimoto, Mutsumi Ito, Seiya Yamayoshi, Samantha Loeber, Masumi Tsuda, Lei Wang, Seiya Ozono, Erika P. Butlertanaka, Yuri L. Tanaka, Ryo Shimizu, Kenta Shimizu, Kumiko Yoshimatsu, Ryoko Kawabata, Takemasa Sakaguchi, Kenzo Tokunaga, Isao Yoshida, Hiroyuki Asakura, Mami Nagashima, Yasuhiro Kazuma, Ryosuke Nomura, Yoshihito Horisawa, Kazuhisa Yoshimura, Akifumi Takaori-Kondo, Masaki Imai, Mika Chiba, Hirotake Furihata, Haruyo Hasebe, Kazuko Kitazato, Haruko Kubo, Naoko Misawa, Nanami Morizako, Kohei Noda, Akiko Oide, Mai Suganami, Miyoko Takahashi, Kana Tsushima, Miyabishara Yokoyama, Yue Yuan, Shinya Tanaka, So Nakagawa, Terumasa Ikeda, Takasuke Fukuhara, Yoshihiro Kawaoka, Kei Sato
Nature 602 ( 7896 ) 300 - 306 2021.11
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
DOI： 10.1038/s41586-021-04266-9
Other Link： https://www.nature.com/articles/s41586-021-04266-9
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Naturally Occurring ZCCHC3 Variants Modulate Antiretroviral Activity in Cynomolgus Macaques Reviewed
Jacob Fadipe, Tomotaka Okamura, Shige H. Yoshimura, Akatsuki Saito
Microbiology and Immunology 2026.9
Authorship：Last author, Corresponding author Publishing type：Research paper (scientific journal) Publisher：Wiley
ABSTRACT
Many mammalian cells restrict viral replication by utilizing various host restriction factors. We recently demonstrated that CCHC‐type zinc‐finger‐containing protein 3 (ZCCHC3) suppresses human immunodeficiency virus type 1 (HIV‐1) replication through multiple mechanisms. We also revealed that single‐nucleotide polymorphisms in human ZCCHC3 affect its antiviral function; however, whether similar genetic and functional diversity is present in other species remains unknown. In this study, we investigated the genetic and functional diversity of ZCCHC3 in cynomolgus macaques, a critical animal model for HIV‐1‐related research. Sequencing analysis of eight independent ZCCHC3 clones per animal revealed substantial amino acid diversity among cynomolgus macaques. We selected 12 representative variants and examined their antiviral activity against several retroviral vectors derived from HIV‐1, simian immunodeficiency virus, feline immunodeficiency virus, and murine leukemia virus. Moreover, using replication‐competent HIV‐1, we showed that selected cynomolgus macaque ZCCHC3 variants can affect both viral production and viral infectivity. These results suggest that the genetic and functional diversity of ZCCHC3 is not limited to humans and underscore the importance of considering ZCCHC3 variation in cynomolgus macaques when using them as animal models for HIV‐1‐related research.Other Link： https://onlinelibrary.wiley.com/doi/full-xml/10.1111/1348-0421.70088
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Wright Andrews Ofotsu Amesimeku, Yoshihiro Nakata, Nami Monde, Hiromi Terasawa, Hirotaka Ode, Hiroyuki Sasaki, Consolata Elias Rukondo, Takeshi Matsui, Perpetual Nyame, Md. Jakir Hossain, Akatsuki Saito, Tomohiro Sawa, Terumasa Ikeda, Yosuke Maeda, Yasumasa Iwatani, Kazuaki Monde
Proceedings of the National Academy of Sciences 123 ( 36 ) 2026.9
Publishing type：Research paper (scientific journal) Publisher：National Academy of Sciences
Lenacapavir (LEN), a potent capsid inhibitor, suppresses reverse transcription and nuclear import by disrupting capsid core formation in HIV type 1 (HIV-1). However, its effects on late-stage viral assembly remain unclear. Although p24 ELISA suggested that LEN substantially inhibited HIV-1, both RT-dPCR and Vpr-HiBiT assays indicated that suppression of viral release required relatively high LEN concentrations. This discrepancy was attributed to LEN-induced reduction in p24 solubility, leading to an overestimation of its inhibitory effect on viral release. In addition, excessive intracellular Gag processing by the viral protease was observed in the presence of LEN, which contributed to impaired viral release. Furthermore, LEN induced numerous abnormal, discrete clusters of Gag at the plasma membrane. Using mass photometry, which was applied to analyze HIV-1 particle sizes, and transmission electron microscopy, we identified heterogeneous LEN-induced viral-like particles (LENiVLPs; diameter >200 nm) containing both Gag and Env proteins. Although LENiVLPs retained membrane fusion capability, they were noninfectious owing to postentry defects that prevented viral replication. Thus, LEN binds to the precursor Gag and promotes aberrant particle formation, offering insights that could guide the development of next-generation LEN-based therapeutics targeting the late stages of the viral life cycle.
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Multi-organ expression of viral entry receptor-related genes in feline fetal tissues Reviewed
Anon H. Kosaka, Zoë K. K. Mia, Nanami X. Kato, Asami Oguro-Ando, Akatsuki Saito
Scientific Reports 2026.8
Authorship：Last author, Corresponding author Publishing type：Research paper (scientific journal) Publisher：Springer Science and Business Media LLC
Abstract
Viral receptors play a key role in determining viral organ tropism and host susceptibility to infection. However, distribution of viral receptor expression across feline organs, particularly during the fetal stage, remains poorly understood. In this study, we examined the expression patterns of multiple viral receptor–related genes across fetal cat organs using quantitative polymerase chain reaction. Gene expression levels were quantified as delta cycle threshold values and analyzed using a linear mixed-effects model to account for repeated measurements from the same individual. Initial data visualization showed considerable variation in delta cycle threshold values across different organs and receptors, along with interindividual variability. Statistical analysis using mixed-effects modeling confirmed significant differences in expression patterns among organs and receptors. Notably, several receptors exhibited pronounced organ-specific expression patterns, whereas others showed broader distributions across tissues. In addition, variability among individuals was observed across organ–receptor combinations, highlighting heterogeneity in receptor expression among fetal samples. These findings indicate that viral receptor expression in feline fetal tissues is both organ-specific and subject to interindividual variation. Overall, these results provide a foundational dataset for understanding routes of viral entry and organ tropism in cats and may support future research into feline viral pathogenesis and vertical transmission. -
Yuika Y HOURI, Nanami X KATO, Yukinobu ADACHI, Miki X KAWANO, Haruka DOSAKA, Takuya HIRAI, Naoyuki FUKE, Yasuyuki KANEKO, Akatsuki SAITO
Journal of Veterinary Medical Science 2026.8
Authorship：Last author, Corresponding author Publishing type：Research paper (scientific journal) Publisher：Japanese Society of Veterinary Science
DOI： 10.1292/jvms.26-0218
Books 【 display / non-display 】
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実験医学
齊藤暁、入江崇.（ Role： Joint author , SARS-CoV-2デルタ株の高病原性メカニズムの解明）
羊土社 2022.5
Language：Japanese Book type：General book, introductory book for general audience
MISC 【 display / non-display 】
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カニクイザルAPOBEC3遺伝子の遺伝的多様性と抗ウイルス活性の解析
麦田友佳, 浦野恵美子, 齊藤暁, 大出裕高, 岩谷靖雅, 保富康宏, 池田輝政
日本エイズ学会誌 26 ( 4 ) 2024
Publishing type：Rapid communication, short report, research note, etc. (scientific journal)
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Genetic and functional diversity of APOBEC3 genes in Cynomolgus monkey (Macaca fascicularis)
麦田友佳, 浦野恵美子, 齊藤暁, 大出裕高, 岩谷靖雅, 保富康宏, 池田輝政
日本ウイルス学会学術集会プログラム・予稿集(Web) 71st 2024
Publishing type：Rapid communication, short report, research note, etc. (scientific journal)
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APOBEC3 proteins are the primary target of HIV-1 Vif for infectious virus production in THP-1, even with type I interferon treatment
清水凌, 清水凌, JONATHAN Michael, JONATHAN Michael, 齊藤暁, 池田輝政
日本ウイルス学会学術集会プログラム・予稿集(Web) 71st 2024
Publishing type：Rapid communication, short report, research note, etc. (scientific journal)
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I型インターフェロンで処理した骨髄性白血病細胞株THP-1において,APOBEC3タンパク質は感染性ウイルス産生のためのHIV-1 Vifの主要な標的である
清水凌, JONATHAN Michael, JONATHAN Michael, 齊藤暁, 池田輝政
日本エイズ学会誌 26 ( 4 ) 2024
Publishing type：Rapid communication, short report, research note, etc. (scientific journal)
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Immune resistance of newly emerging SARS-CoV-2 Omicron variants
瓜生慧也, 伊東潤平, SHOFA Maya, 清水凌, BEGUM MST Monira, 山岨大智, PLIANCHAISUK Arnon, 藤田滋, 小杉優介, 齊藤暁, 池田輝政, 佐藤佳
日本ウイルス学会学術集会プログラム・予稿集(Web) 70th 2023
Publishing type：Rapid communication, short report, research note, etc. (scientific journal)
Presentations 【 display / non-display 】
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Species tropism of Orthohepadnavirus Invited International conference
Akatsuki Saito
The 3rd France-Japan symposium on HIV/AIDS and infectious disease basic research
Event date： 2024.10.28 - 2024.10.30
Presentation type：Symposium, workshop panel (nominated)
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霊長類感染病態モデルを用いたウイルス学研究
齊藤暁
第167回日本獣医学会学術集会
Event date： 2024.9.10 - 2024.9.13
Presentation type：Symposium, workshop panel (nominated)
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抗レトロウイルス宿主因子である哺乳類TRIM5の遺伝的、機能的多様性 International conference
齊藤暁
日本遺伝学会第96回高知大会
Event date： 2024.9.4 - 2024.9.6
Presentation type：Symposium, workshop panel (nominated)
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Species tropism of Orthohepadnavirus Invited International conference
Akatsuki Saito
Japan-UK International Research project
Event date： 2024.7.29
Presentation type：Symposium, workshop panel (nominated)
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Host ZCCHC3 blocks HIV-1 infection and production by a dual mechanism International conference
Akatsuki Saito
United States - Japan Cooperative Medical Sciences Program, 22nd International Conference on Emerging Infectious Diseases in the Pacific Rim, AIDS Panel
Event date： 2024.3.8 - 2024.3.9
Presentation type：Symposium, workshop panel (nominated)
Industrial property rights 【 display / non-display 】
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ブタ由来培養細胞、及びそれを用いたウイルス増殖方法及びウイルス分離方法
齊藤 暁、田中友理
Applicant：宮崎大学
Application no：2024-178528 Date applied：2024.10.11
Announcement no：2025-067879 Date announced：2025.4.24
Country of applicant：Domestic
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臨床検体からのウイルスの分離方法
齊籐 暁、Maya Shofa
Applicant：宮崎大学
Application no：2023-051037 Date applied：2023.3.28
Announcement no：2024-140050 Date announced：2024.10.10
Country of applicant：Domestic
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ＳＡＲＳ－ＣｏＶ－２を含む広範なウイルス感染症に対する治療薬
齊藤 暁, 岡本 徹, 宮本 洋一, 岡 正啓
Applicant：国立大学法人 宮崎大学, 国立大学法人 大阪大学, 国立研究開発法人医薬基盤・健康・栄養研究所
Application no：2023-511333 Date applied：2022.3.29
Announcement no：WO2022/210644 Date announced：2023.9.28
Country of applicant：Domestic , International (PCT) application
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ＳＡＲＳ－ＣｏＶ－２を含む広範なウイルス感染症に対する治療薬
齊藤 暁、岡本 徹、宮本 洋一、岡 正啓
Applicant：宮崎大学、大阪大学、医薬基盤・健康・栄養研究所
Application no：PCT/JP2022/015292 Date applied：2022.3.29
Announcement no：WO2022/210644 A1 Date announced：2022.10.6
Awards 【 display / non-display 】
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ECC山口メモリアルエイズ研究奨励賞
2025.12 日本エイズ学会
齊藤暁
Award type：International academic award (Japan or overseas)
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最優秀発表賞＜症例報告部門＞
2025.11 JSFM第12回猫の集会 保護猫施設内でアウトブレイクが認められた猫ヘパドナウイルスの ウイルス学的、病理学的解析
加藤七海, 銅坂悠, 足立行庸, 金子泰之, 平井卓哉, 福家直幸, 齊藤暁
Award type：International academic award (Japan or overseas)
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最優秀発表賞＜キャットフレンドリー部門＞
2025.11 JSFM第12回猫の集会 皮脂を用いたネコ RNA ウイルス感染症診断方法の確立
福嶋優莉, 齊藤波子, 目堅博久, 齊藤暁
Award type：International academic award (Japan or overseas)
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CADIC最優秀論文賞
2024.7 宮崎大学産業動物防疫リサーチセンター（CADIC）
齊藤暁
Award type：International academic award (Japan or overseas)
Grant-in-Aid for Scientific Research 【 display / non-display 】
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マダニが保有するＭ分節欠損フェヌイウイルスの生物学的意義の解明
Grant number：25K22364 2025.04 - 2027.03
独立行政法人日本学術振興会 科学研究費基金 挑戦的研究（萌芽）
Authorship：Coinvestigator(s)
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Elucidation of species specificity of Jembrana disease virus
Grant number：24K09227 2024.04 - 2027.03
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
Authorship：Principal investigator
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動物フォーミーウイルスベクターの基盤構築と牛伝染性リンパ腫ワクチンの開発
Grant number：23K23765 2024.04 - 2026.03
独立行政法人日本学術振興会 科学研究費基金 基盤研究(B)
Authorship：Coinvestigator(s)
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ゲノムワイドスクリーニングに基づくSFTS発症小動物モデルの樹立と治療開発展開
Grant number：23K21273 2024.04 - 2026.03
独立行政法人日本学術振興会 科学研究費基金 基盤研究(B)
Authorship：Coinvestigator(s)
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Promotion of comprehensive interdisciplinary virology for the post-COVID era
Grant number：23K20041 2023.04 - 2030.03
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Fund for the Promotion of Joint International Research (International Leading Research )
Authorship：Coinvestigator(s)