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Affiliation |
Faculty of Agriculture Region of Veterinary Science |
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Associate Professor |
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Related SDGs |
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IRIE Takao
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Papers 【 display / non-display 】
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A Case of Fasciolosis Reinfection in Japan Reviewed
Akiyoshi Kumiko, Kokubo-Tanaka Mio, Yamauchi Shuichiro, Yonezu Keisuke, Abe Ichitaro, Tawara Katsunori, Saito Shotaro, Kondo Hidekazu, Fukuda Tomoko, Fukui Akira, Akioka Hidefumi, Shinohara Tetsuji, Teshima Yasushi, Yufu Kunio, Shimono Nobuyuki, Mizuno Yusuke, Irie Takao, Yoshida Ayako, Takahashi Naohiko, Maruyama Haruhiko
Internal Medicine advpub ( 0 ) 2025.8
Language：English Publishing type：Research paper (scientific journal) Publisher：The Japanese Society of Internal Medicine
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Strongyle nematode fauna in three ruminants in upper northern Thailand Reviewed International coauthorship International journal
Thanakorn Rompo, Naoki Hayashi, Ernest Teo, Tawatchai Singhla, Chakorn Kunkaew, Duanghatai Sripakdee, Boondarika Nambooppha, Saruda Wanganurakkul, Kanthanis Limwibulpong, Kanyatip Sangarun, Napatsorn Suwongsaksri, Saravalee Suphakarn, Chanakan Chotiphutthikul, Yuto Matsui, Takao Irie, Ayako Yoshida, Lerdchai Chintapitaksakul, Naoaki Misawa, Nariaki Nonaka, Ryo Nakao, Saruda Tiwananthagorn
Parasitology International 108 103057 - 103057 2025.3
Language：English Publishing type：Research paper (scientific journal) Publisher：Elsevier BV
Gastrointestinal parasites, particularly strongyle nematodes, pose a significant threat to the health of ruminants. Due to the technical limitations of microscopic and conventional PCR-based methods, the strongyle parasite fauna has not been well studied even in common livestock animals. The present study aimed to investigate the prevalence and diversity of strongyle nematodes in three ruminant species in northern Thailand using a nemabiome approach. Fecal samples were collected from buffaloes, cattle, and goats that were raised for meat and for dairy in four provinces in northern Thailand. Strongyle infections were determined using egg flotation and McMaster techniques followed by DNA metabarcoding for species identification. The results showed high prevalence of strongyles especially in goats raised for meat (88 %), and in goats raised for dairy (72 %). Significantly more goats and cattle raised for meat were strongyle egg-positive compared to their dairy counterparts. Notably, deworming frequency was not significantly associated with strongyle egg-positivity in all ruminant groups apart from dairy goats. Nemabiome analysis identified 11 strongyle species across seven genera. Among the ruminant hosts, beef cattle exhibited the highest strongyle richness. Additionally, the dominance of specific strongyle species influenced the differences observed in diversity indices. This research is the first to apply the nemabiome approach to assess strongyle nematode diversity in northern Thailand, providing valuable insights into nematode community compositions. These findings emphasize the importance of molecular techniques for parasite monitoring and the development of targeted control strategies.
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Narrowing the region of candidate genes that control the development of protoscoleces of Echinococcus multilocularis in the mouse liver. Reviewed International journal
Sato Keisuke, Hikosaka Naritaka, Kouguchi Hirokazu, Irie Takao, Morimatsu Masami, Agui Takashi
Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases 127 105704 - 105704 2025.1
Language：English Publishing type：Research paper (scientific journal)
Alveolar echinococcosis is a zoonosis caused by the larval stage of Echinococcus multilocularis. In previous studies, QTL analysis using C57BL/6 N (B6) and DBA/2 (D2) which differ in susceptibility suggested the presence of genes on chromosome 1 that control protoscolex development. In this study, we constructed several congenic mice with different chromosome 1 regions substituted to confirm the presence of responsible genes and to narrow down the regions where the responsible genes exist. Five lines of third-generation congenic strain were constructed and infection experiments were conducted. The results showed that the development of protoscolex was seen in the two lines, resulting to narrow-down the responsible region between 69.4 cM and 70.67 cM. There were 18 genes having different SNPs and 10 genes having amino acid substitutions between B6 and D2 within this region. Infection experiments with third-generation of congenic mice succeeded in narrowing down the chromosomal region determining protoscolex development, resulting to reduce the number of candidate genes. The identification of the gene responsible for protoscolex development will contribute to the control of E. multilocularis infection.
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Mitogenomic exploration supports the historical hypothesis of anthropogenic diffusion of a zoonotic parasite Echinococcus multilocularis Reviewed International journal
Naoki Hayashi, Ryo Nakao, Yuma Ohari, Takao Irie, Hirokazu Kouguchi, Elisha Chatanga, Wessam Mohamed Ahmed Mohamed, Mohamed Abdallah Mohamed Moustafa, Gohta Kinoshita, Munehiro Okamoto, Kinpei Yagi, Nariaki Nonaka
iScience 26 ( 10 ) 107741 - 107741 2023.10
Language：English Publishing type：Research paper (scientific journal) Publisher：Elsevier BV
Animal movement across regions owing to human activity can lead to the introduction of pathogens, resulting in disease epidemics with medical and socioeconomic significance. Here, we validated the hypothesis that human activity, such as the transportation of infected animals, has played a significant role in introducing the zoonotic parasite Echinococcus multilocularis into Hokkaido, Japan, by synthesizing and evaluating parasite genetic data in light of historical records. Our analysis indicates that a major genetic group in Hokkaido originated from St. Lawrence Island, USA, which is in accordance with the route suggested by historical descriptions. Moreover, we identified a minor genetic group closely related to parasites found in Sichuan, China. This fact implies that parasite invasion in Japan may result from complex and inadvertent animal translocations. These findings emphasize the anthropogenic impacts on zoonotic parasite spread and provide a crucial perspective for preventing future potential epidemics.
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Current Status and Issues of Utilization of Wild Animal Meat for Pet Food in Japan Reviewed
TAKAI Shinji, SUZUKI Yasunori, KABEYA Hidenori, ANDOH Masako, IRIE Takao, YAMAZAKI Akiko, UNE Yumi, SUGIYAMA Hiromu, ASAKURA Hiroshi, MAEDA Ken
Journal of the Japan Veterinary Medical Association 76 ( 9 ) e213 - e225 2023.9
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Japan Veterinary Medical Association
Books 【 display / non-display 】
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獣医公衆衛生学
伊藤直人、上野俊治、壁谷英則、苅和宏明、佐藤至、丸山総一、三澤尚明、三宅眞実、森田幸雄（ Role： Contributor）
文永堂出版 2024.3
Language：Japanese Book type：Textbook, survey, introduction
MISC 【 display / non-display 】
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Echinococcus ortleppi infection in humans: An emerging zoonosis in Asia. Reviewed International coauthorship International journal
Ayako Yoshida, Takao Irie, Lan-Anh Le, Khanh-Linh Bui, Yukifumi Nawa
Parasitology international 103 102949 2024.12
Language：English Publishing type：Article, review, commentary, editorial, etc. (scientific journal)
Echinococcus ortleppi is the genotype G5 of Echinococcus granulosus sensu lato and is a zoonotic canine tapeworm of which larvae causes cystic diseases in domestic animals and also humans. While this species is highly endemic and widely spread in domestic animals, human infection is extremely rare and only sporadic, and thus, entire picture of human cystic echinococcosis due to infection with E. ortleppi is unclear. We have made an extensive literature review on the cases of E. ortleppi infection in humans and found a total of 19 cases from various places in the world with recent emergence in Asian countries.
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Current Status and Issues of Utilization of Wild Animal Meat for Pet Food in Japan
高井伸二, 鈴木康規, 壁谷英則, 安藤匡子, 入江隆夫, 山崎朗子, 宇根有美, 杉山広, 朝倉宏, 前田健
日本獣医師会雑誌(Web) 76 ( 9 ) 2023
Publishing type：Rapid communication, short report, research note, etc. (scientific journal)
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Considering the assembly methods for the draft genome of Echinococcus multilocularis using the long-read sequencer MinION
今里裕平, 中尾亮, 孝口裕一, 入江隆夫, 八木欣平, 野中成晃
日本寄生虫学会大会プログラム・抄録集 89th 2020
Publishing type：Rapid communication, short report, research note, etc. (scientific journal)
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多包条虫卵の殺卵処理による比重変化とMini-FLOTAC装置を利用した検査法の評価
紀田泉, 孝口裕一, 入江隆夫, 八木欣平, 中尾亮, 野中成晃
日本獣医学会学術集会講演要旨集 163rd 2020
Publishing type：Rapid communication, short report, research note, etc. (scientific journal)
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Comparative analysis on the migration dynamics of Echinococcus multilocularis oncospheres in two mouse strains
林直樹, 中尾亮, 孝口裕一, 今里裕平, 入江隆夫, 八木欣平, 野中成晃
日本寄生虫学会大会プログラム・抄録集 89th 2020
Publishing type：Rapid communication, short report, research note, etc. (scientific journal)
Presentations 【 display / non-display 】
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Toxoplasma gondiiによる食肉汚染検出を目的としたリアルタイムPCR法によるon-site検査の開発
鈴木こころ、Wui Ho Hung、入江隆夫、三谷康正、西澤尚文、吉田彩子
第94回日本寄生虫学会大会
Event date： 2025.3.18 - 2025.3.19
Language：Japanese Presentation type：Oral presentation (general)
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イノシシで成熟できる単為生殖型肝蛭のミトコンドリアゲノム配列による集団遺伝学的解析
水野雄介, 入江隆夫, 水村匡伸, 吉田彩子
第94回日本寄生虫学会大会
Event date： 2025.3.18 - 2025.3.19
Language：Japanese Presentation type：Oral presentation (general)
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多包虫症の新規検査法の標的としての宿主血中循環セルフリーDNAの評価
入江 隆夫、伊東 拓也、後藤 明子、中尾 亮、迫 康仁、水村 匡伸、長野 礼太郎、吉田 彩子、山野 公明
第94回日本寄生虫学会大会
Event date： 2025.3.18 - 2025.3.19
Language：Japanese Presentation type：Oral presentation (general)
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宮崎県内における猫の犬糸状虫感染状況調査
宮﨑 ありさ, 入江 隆夫, 日髙 千佳子, 吉田 彩子
第76回 日本寄生虫学会 南日本支部大会 第73回 日本衛生動物学会 南日本支部大会 合同大会
Event date： 2024.10.12 - 2024.10.13
Language：Japanese Presentation type：Oral presentation (general)
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ネズミ糞線虫およびベネズエラ糞線虫宿主体内移行経路の検討
加藤さらさ、田中美緒、Safira Rosalina、Mari Ishida、丸山治彦、入江隆夫、吉田彩子
第76回 日本寄生虫学会 南日本支部大会 第73回 日本衛生動物学会 南日本支部大会 合同大会
Event date： 2024.10.12 - 2024.10.13
Language：Japanese Presentation type：Oral presentation (general)
Awards 【 display / non-display 】
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第13回 日本獣医寄生虫学奨励賞
2022.9 日本獣医寄生虫学会 エキノコックス症制御のための終宿主動物対策
入江隆夫
Award type：Award from Japanese society, conference, symposium, etc.
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令和４年度獣医学術九州地区学会（獣医公衆衛生学会）九州地区獣医師会連合会会長賞
2022.9 九州地区獣医師会連合会 九州産ジビエの住肉胞子虫の保有状況と流行種
渡部峻也 、中村鉄平 、市居修 、吉田彩子 、入江隆夫
Award type：Award from Japanese society, conference, symposium, etc.
Grant-in-Aid for Scientific Research 【 display / non-display 】
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野生鳥獣の食肉利用に関わるリスク分析に資する研究
Grant number：24KA1004 2024.04 - 2027.03
厚生労働省 令和6年度厚生労働科学研究費補助金（食品の安全確保推進研究） 応用研究
Authorship：Coinvestigator(s)
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寄生虫由来の宿主コミュニケーション因子 血中循環核酸の網羅的同定と診断利用
Grant number：21K16325 2021.04 - 2025.03
日本学術振興会 科学研究費基金 若手研究
入江 隆夫
Authorship：Principal investigator
第一年度は特にエキノコックスを対象として研究を実施した。多包条虫卵を感染させたコットンラットの、感染後２か月（5検体）および５か月以降（４検体）の血液からccfDNAを抽出し、濃度を確認後、次世代シークエンス解析に供した。得られたccfDNA量は血漿１ mlから0.6 ng～11.5 ng と微量かつばらつきがあったが、すべての検体でショットガンシークエンスが可能であった。各検体について約500万リードを得て、多包条虫のゲノム配列にマッピングを行ったところ、0.5%ほどの配列が相同性を示した。このうち、多包条虫特異的であり、かつリード数が多いものを選別し、リアルタイムPCRでの検出系を検討している。現時点では診断系の候補配列の決定には至っていないが、検討したうちの１配列では、多包虫が十分に成熟して原頭節の形成のみられる感染５か月以降の検体において、極めて高感度に検出可能となるリアルタイムPCR系が得られている。引き続き他の配列についても評価を行い、感染初期を含む全期間にわたり検出可能となる検出系を構築を目指す。
肺吸虫については、実験感染に用いる肺吸虫種を、当初予定していた大平肺吸虫から、人獣共通種である宮崎肺吸虫に変更した。疫学調査により、当該種のメタセルカリアを保有する淡水産カニの分布地を特定したので、第二年度にはラットへの実験感染、血液検体の採取とccfDNAの抽出、そしてショットガンシークエンスを実施する。
野生動物の疫学調査の過程で、タヌキのウエステルマン肺吸虫３倍体、アナグマの宮崎肺吸虫の感染例を見出した。加えて、当該タヌキの取得地点に近接する川で、中間宿主であるモクズガニを捕獲してメタセルカリアの保有状況を調査したところ、高率かつ濃厚なウエステルマン肺吸虫３倍体メタセルカリアの感染を認め、同地点で確かに生活環が維持されていることを示した。 -
野生鳥獣由来食肉の食中毒発生防止と衛生管理ガイドラインの改良に資する研究
Grant number：21KA0401 2021.04 - 2024.03
厚生労働省 食品の安全確保推進研究事業 応用研究
Authorship：Collaborating Investigator(s) (not designated on Grant-in-Aid)
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Disease control of Echinococcus multilocularis by development of anti-echinococcus drug and vaccine-bait for both definitive and intermediate hosts
Grant number：17K08815 2017.04 - 2020.03
Japan Society for the Promotion of Science Grant-in-Aid for Scientific Research Grant-in-Aid for Scientific Research(C)
Kouguchi Hirokazu
Authorship：Coinvestigator(s)
In this research, we showed that atovaquone (ATV), a commercially available drug, inhibited mitochondrial complex III activities of E. multilocurlaris. The culture experiments revealed the ability of ATV to kill protoscoleces. Additionally, we demonstrated that oral administration of ATV significantly reduced primary hydatid cyst development in the mouse liver, indicating that complex III is one of a promising target for development of anti-echinococal drug. To understand mechanisms of adult worm exclusion in dogs infected with E. multilocularis, we tried to induce host protective immune responses against adult worm by long-term oral administration of adult worm antigens. As a result, around half of dogs examined in this study showed adult worm exclusion largely, however the other half of dogs did not showed significant adult worm exclusion. To reinforce this exclusion, it was considered to be a strong immunization technique was needed for induction of host protection immunity in dogs.
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An epidemiological study on echinococcosis in Qinghai, China, and development of a chemotherapeutic drug for humans and domestic animals
Grant number：26304041 2014.04 - 2017.03
Grant-in-Aid for Scientific Research Grant-in-Aid for Scientific Research(B)
Nonaka Nariaki, HORII Yoichiro, KOUGUCHI Hirokazu, IRIE Takao, LEE Wei, GUO ZhiHong, NI Ma, ENKAI Shigehiro, KAI Feng
In order to contribute to the echinococcosis control in Qinghai province, China, an epidemiological survey and a deworming trial were conducted. Since the feces of foxes, one of the most important definitive hosts of Echinococcus multilocularis, were deposited away from the residence area of nomad people, the risk of E. multilocularis transmission from foxes to humans was assumed to be low. Although a deworming trial was conducted to evaluate the effect of education on deworiming conductivity, a national deworming trial of dogs was started in the last year at our study site, thus, the effect of education could not be elucidated. In parallel to this, a new chemotherapeutic drug for echinococcosis was developed against mitochondrial respiratory chain of the parasite and an effective compound that reduced the cyst development of E. multilocularis was discovered.