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Faculty of Agriculture Region of Veterinary Science |
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Associate Professor |
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Related SDGs |
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IRIE Takao
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Papers 【 display / non-display 】
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Three-dimensional structure and epithelial changes of the piriform sinus fistula in cotton rats Reviewed
Teppei Nakamura, Marina Hosotani, Osamu Ichii, Takao Irie, Akio Shinohara, Hirokazu Kouguchi, Yasuhiro Kon
Cell and Tissue Research 403 ( 3 ) 2026.3
Language：English Publishing type：Research paper (scientific journal) Publisher：Springer Science and Business Media LLC
DOI： 10.1007/s00441-026-04056-7
Other Link： https://link.springer.com/article/10.1007/s00441-026-04056-7
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Three-dimensional structure and epithelial changes of the piriform sinus fistula in cotton rats Reviewed
入江 隆夫, 篠原 明男
Cell and tissue research 403 ( 3 ) 27 2026.2
Language：English Publishing type：Research paper (scientific journal) Publisher：Springer Science and Business Media LLC
Piriform sinus fistula (PSF) is a rare congenital anomaly derived from the third or fourth pharyngeal pouch. However, its developmental mechanisms remain poorly understood owing to the absence of suitable animal models. Cotton rats (Sigmodon hispidus) spontaneously develop PSF, providing a unique opportunity to investigate its anatomical origin and postnatal remodeling. In this study, we performed a three-dimensional reconstruction of serial histological sections from two inbred strains, HIS/Mz with third-pouch–derived PSF and HIS/Hiph with fourth-pouch–derived PSF. The third-pouch PSF opened dorsally at the base of the piriform sinus and terminated outside the thyroid gland, whereas the fourth-pouch PSF opened ventrally at the apex and extended into the thyroid parenchyma. Focusing on the fourth-pouch PSF in the HIS/Hiph, we further identified progressive epithelial stratification and age-dependent inflammation leading to suppurative thyroiditis. This remodeling was accompanied by marked alterations in epithelial marker expression, including a shift from Nkx2-1+/CK8+/UEA-I− to p63+/CK5+/UEA-I+ cell populations. Together, these features resemble the known characteristics of ultimobranchial body remnants and highlight the dynamic inflammation-associated transformation of the PSF epithelium. Collectively, our findings establish the cotton rat as a biologically informative experimental animal model for elucidating the developmental origin and pathological progression of PSF. This study provides new insights that may improve understanding, diagnosis, and clinical management of congenital cervical anomalies in humans.
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Ishida M, Irie T, Tanaka R, Maruyama H, Yoshida A
Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases 135 105839 2025.10
Language：English Publishing type：Research paper (scientific journal) Publisher：Infection Genetics and Evolution
Strongyloides ratti is an intestinal nematode commonly found in rats. Unlike other Strongyloides species, the tissue-migrating third-stage larvae in S. ratti follow a unique route of invasion via the nasofrontal region before reaching the gut. Despite its importance in host invasion, the transcriptomic profile of this larval stage has not been characterized. In this study, we performed RNA sequencing (RNA-seq) to examine gene expression in head-derived tissue-migrating third-stage larvae (hL3) and infective third-stage larvae (iL3) of the S. ratti Tokyo strain. hL3 were collected from rat heads at 30 h post-infection. Differential expression analysis revealed 664 upregulated genes in hL3. Functional annotation showed enrichment of genes encoding astacin metalloproteases and sperm-coating protein/Tpx-1/Ag5/PR-1/Sc7 (SCP/TAPS) protein families—both associated with tissue invasion and immune modulation. Quantitative RT-PCR was used to validate selected differentially expressed genes. Seven hL3-specific astacin genes were identified, of which six belonged to the M12A group. One hL3-specific astacin gene showed domain similarity to strongylastacin, a known tissue-penetration protein. Two SCP/TAPS genes were unique to hL3 and were absent from parasitic females, suggesting distinct roles in larval migration. By contrast, G protein-coupled receptor genes, particularly those related to chemosensory functions, were not upregulated in hL3, indicating that these pathways may be less important during this stage. These results provide the first transcriptomic profile of hL3 in S. ratti, and identify potential molecular mechanisms driving larval migration and immune evasion during host infection.
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Echinococcus infection in domestic and zoo animals Reviewed
The Journal of Farm Animal in Infectious Disease 14 ( 3 ) 39 - 47 2025.9
Authorship：Lead author, Corresponding author Language：Japanese Publishing type：Research paper (scientific journal)
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A Case of Fasciolosis Reinfection in Japan Reviewed
Akiyoshi Kumiko, Kokubo-Tanaka Mio, Yamauchi Shuichiro, Yonezu Keisuke, Abe Ichitaro, Tawara Katsunori, Saito Shotaro, Kondo Hidekazu, Fukuda Tomoko, Fukui Akira, Akioka Hidefumi, Shinohara Tetsuji, Teshima Yasushi, Yufu Kunio, Shimono Nobuyuki, Mizuno Yusuke, Irie Takao, Yoshida Ayako, Takahashi Naohiko, Maruyama Haruhiko
Internal Medicine advpub ( 0 ) 921 - 925 2025.8
Language：English Publishing type：Research paper (scientific journal) Publisher：The Japanese Society of Internal Medicine
Fasciolosis is a major cause of food-borne parasitic zoonosis. It primarily affects ruminants but also infects humans. Clinically, it is characterized by abnormal liver images with prominent eosinophilia. We herein report a 77-year-old Japanese livestock farmer, who experienced fasciolosis reinfection. Environmental investigation of his residence revealed that both intermediate host snails and sika deer, a natural final host, inhabited his house. In addition, cattle were infected with <i>Fasciola</i>. Based on these findings, we concluded that his work place was accidentally contaminated with <i>Fasciola</i> metacercariae.
Books 【 display / non-display 】
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寄生虫の事典
永宗 喜三郎・新倉 保・神川 龍馬・常盤 俊大・彦坂 健児・松崎 素道・脇 司（ Role： Contributor , エキノコックス）
朝倉書店 2026.6
Language：Japanese Book type：Dictionary, encyclopedia
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獣医公衆衛生学
伊藤直人、上野俊治、壁谷英則、苅和宏明、佐藤至、丸山総一、三澤尚明、三宅眞実、森田幸雄（ Role： Contributor）
文永堂出版 2024.3
Language：Japanese Book type：Textbook, survey, introduction
MISC 【 display / non-display 】
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Echinococcus ortleppi infection in humans: An emerging zoonosis in Asia. Reviewed International coauthorship International journal
Ayako Yoshida, Takao Irie, Lan-Anh Le, Khanh-Linh Bui, Yukifumi Nawa
Parasitology international 103 102949 2024.12
Language：English Publishing type：Article, review, commentary, editorial, etc. (scientific journal)
Echinococcus ortleppi is the genotype G5 of Echinococcus granulosus sensu lato and is a zoonotic canine tapeworm of which larvae causes cystic diseases in domestic animals and also humans. While this species is highly endemic and widely spread in domestic animals, human infection is extremely rare and only sporadic, and thus, entire picture of human cystic echinococcosis due to infection with E. ortleppi is unclear. We have made an extensive literature review on the cases of E. ortleppi infection in humans and found a total of 19 cases from various places in the world with recent emergence in Asian countries.
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ゲノミクスが解き明かす日本のエキノコックスの正体:エキノコックス症コントロールを目指した集団遺伝学的アプローチ
林直樹, 中尾亮, 尾針由真, 水谷雄基, SANGARUN Kanyatip, 入江隆夫, 孝口裕一, 日高正人, 松山紘之, 木下豪太, 岡本宗裕, 八木欣平, 野中成晃
日本寄生虫学会大会プログラム・抄録集 93rd 2024
Publishing type：Rapid communication, short report, research note, etc. (scientific journal)
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北海道における多包条虫Echinococcus multilocularisの集団遺伝構造
林直樹, 中尾亮, 尾針由真, 水谷雄基, SANGARUN Kanyatip, 入江隆夫, 孝口裕一, 日高正人, 松山紘之, 木下豪太, 岡本宗裕, 八木欣平, 野中成晃
日本獣医学会学術集会講演要旨集 167th 2024
Publishing type：Rapid communication, short report, research note, etc. (scientific journal)
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Current Status and Issues of Utilization of Wild Animal Meat for Pet Food in Japan
高井伸二, 鈴木康規, 壁谷英則, 安藤匡子, 入江隆夫, 山崎朗子, 宇根有美, 杉山広, 朝倉宏, 前田健
日本獣医師会雑誌(Web) 76 ( 9 ) 2023
Publishing type：Rapid communication, short report, research note, etc. (scientific journal)
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Considering the assembly methods for the draft genome of Echinococcus multilocularis using the long-read sequencer MinION
今里裕平, 中尾亮, 孝口裕一, 入江隆夫, 八木欣平, 野中成晃
日本寄生虫学会大会プログラム・抄録集 89th 2020
Publishing type：Rapid communication, short report, research note, etc. (scientific journal)
Presentations 【 display / non-display 】
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食肉からのトリヒナ属線虫の検出を目的としたリアルタイムPCR法の確立
三浦帆夏、吉田彩子、入江隆夫
第169回日本獣医学会学術集会 2026.9.8
Event date： 2026.9.8 - 2026.9.11
Language：Japanese Presentation type：Oral presentation (general)
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千葉県における野生動物感染肝蛭の分子系統解析
合田花爽、関まどか、陳雪、坂本寛和、中村ふくみ、筏井宏実、小泉慎一郎、吉田彩子、入江隆夫、彦坂健児
第95回日本寄生虫学会大会
Event date： 2026.3.21 - 2026.3.22
Language：Japanese Presentation type：Poster presentation
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Transcriptome analysis reveals unique gene expression profiles in tissue-migrating Strongyloides ratti larvae
Mari Ishida, Takao Irie, Ryusei Tanaka, Haruhiko Maruyama, Ayako Yoshid
第95回日本寄生虫学会大会
Event date： 2026.3.21 - 2026.3.22
Language：English Presentation type：Oral presentation (general)
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患者および動物由来肝蛭の集団遺伝構造
入江隆夫, 水野雄介, 水村匡伸, 小久保（田中）美緒, 丸山治彦, 吉田彩子
第77回 日本寄生虫学会 南日本支部大会 第74回 日本衛生動物学会 南日本支部大会 合同大会
Event date： 2025.10.10 - 2025.10.11
Language：Japanese Presentation type：Oral presentation (general)
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外来生物スウィンホーキノボリトカゲ（Diploderma swinhonis）に寄生するStrongyluris calotisの分子系統解析による侵入経路の検討
林歩花, 水村匡伸, 入江隆夫, Chen Hsuawien, 保田昌宏, 今竹翔一朗, 槐真史, 岩下歩叶, 吉田彩子
第77回 日本寄生虫学会 南日本支部大会 第74回 日本衛生動物学会 南日本支部大会 合同大会
Event date： 2025.10.10 - 2025.10.11
Language：Japanese Presentation type：Oral presentation (general)
Awards 【 display / non-display 】
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第13回 日本獣医寄生虫学奨励賞
2022.9 日本獣医寄生虫学会 エキノコックス症制御のための終宿主動物対策
入江隆夫
Award type：Award from Japanese society, conference, symposium, etc.
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令和４年度獣医学術九州地区学会（獣医公衆衛生学会）九州地区獣医師会連合会会長賞
2022.9 九州地区獣医師会連合会 九州産ジビエの住肉胞子虫の保有状況と流行種
渡部峻也 、中村鉄平 、市居修 、吉田彩子 、入江隆夫
Award type：Award from Japanese society, conference, symposium, etc.
Grant-in-Aid for Scientific Research 【 display / non-display 】
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血液1滴で寄生虫検査 寄生虫由来宿主血中セルフリーDNAを標的とした新規検査法
Grant number：26K18618 2026.04 - 2029.03
日本学術振興会 科学研究費基金 若手研究
入江 隆夫
Authorship：Principal investigator
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野生鳥獣の食肉利用に関わるリスク分析に資する研究
Grant number：24KA1004 2024.04 - 2027.03
厚生労働省 令和6年度厚生労働科学研究費補助金（食品の安全確保推進研究） 応用研究
Authorship：Coinvestigator(s)
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寄生虫由来の宿主コミュニケーション因子 血中循環核酸の網羅的同定と診断利用
Grant number：21K16325 2021.04 - 2025.03
日本学術振興会 科学研究費基金 若手研究
入江 隆夫
Authorship：Principal investigator
第一年度は特にエキノコックスを対象として研究を実施した。多包条虫卵を感染させたコットンラットの、感染後２か月（5検体）および５か月以降（４検体）の血液からccfDNAを抽出し、濃度を確認後、次世代シークエンス解析に供した。得られたccfDNA量は血漿１ mlから0.6 ng～11.5 ng と微量かつばらつきがあったが、すべての検体でショットガンシークエンスが可能であった。各検体について約500万リードを得て、多包条虫のゲノム配列にマッピングを行ったところ、0.5%ほどの配列が相同性を示した。このうち、多包条虫特異的であり、かつリード数が多いものを選別し、リアルタイムPCRでの検出系を検討している。現時点では診断系の候補配列の決定には至っていないが、検討したうちの１配列では、多包虫が十分に成熟して原頭節の形成のみられる感染５か月以降の検体において、極めて高感度に検出可能となるリアルタイムPCR系が得られている。引き続き他の配列についても評価を行い、感染初期を含む全期間にわたり検出可能となる検出系を構築を目指す。
肺吸虫については、実験感染に用いる肺吸虫種を、当初予定していた大平肺吸虫から、人獣共通種である宮崎肺吸虫に変更した。疫学調査により、当該種のメタセルカリアを保有する淡水産カニの分布地を特定したので、第二年度にはラットへの実験感染、血液検体の採取とccfDNAの抽出、そしてショットガンシークエンスを実施する。
野生動物の疫学調査の過程で、タヌキのウエステルマン肺吸虫３倍体、アナグマの宮崎肺吸虫の感染例を見出した。加えて、当該タヌキの取得地点に近接する川で、中間宿主であるモクズガニを捕獲してメタセルカリアの保有状況を調査したところ、高率かつ濃厚なウエステルマン肺吸虫３倍体メタセルカリアの感染を認め、同地点で確かに生活環が維持されていることを示した。 -
野生鳥獣由来食肉の食中毒発生防止と衛生管理ガイドラインの改良に資する研究
Grant number：21KA0401 2021.04 - 2024.03
厚生労働省 食品の安全確保推進研究事業 応用研究
Authorship：Collaborating Investigator(s) (not designated on Grant-in-Aid)
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Disease control of Echinococcus multilocularis by development of anti-echinococcus drug and vaccine-bait for both definitive and intermediate hosts
Grant number：17K08815 2017.04 - 2020.03
Japan Society for the Promotion of Science Grant-in-Aid for Scientific Research Grant-in-Aid for Scientific Research(C)
Kouguchi Hirokazu
Authorship：Coinvestigator(s)
In this research, we showed that atovaquone (ATV), a commercially available drug, inhibited mitochondrial complex III activities of E. multilocurlaris. The culture experiments revealed the ability of ATV to kill protoscoleces. Additionally, we demonstrated that oral administration of ATV significantly reduced primary hydatid cyst development in the mouse liver, indicating that complex III is one of a promising target for development of anti-echinococal drug. To understand mechanisms of adult worm exclusion in dogs infected with E. multilocularis, we tried to induce host protective immune responses against adult worm by long-term oral administration of adult worm antigens. As a result, around half of dogs examined in this study showed adult worm exclusion largely, however the other half of dogs did not showed significant adult worm exclusion. To reinforce this exclusion, it was considered to be a strong immunization technique was needed for induction of host protection immunity in dogs.
Available Technology 【 display / non-display 】
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野生獣肉「ジビエ」の保有する寄生虫の調査
寄生虫由来の核酸を標的とした検査方法の開発Message：お気軽にお問合せください。