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Faculty of Medicine School of Medicine Department of Medical Sciences, Pharmacology |
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Assistant Professor |
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MIURA Ayako
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Research Areas 【 display / non-display 】
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Life Science / Respiratory medicine
Papers 【 display / non-display 】
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The NERP-4–SNAT2 axis regulates pancreatic β-cell maintenance and function Reviewed International coauthorship
Weidong Zhang, Ayako Miura, Md Moin Abu Saleh, Koichiro Shimizu, Yuichiro Mita, Ryota Tanida, Satoshi Hirako, Seiji Shioda, ValeryGmyr, Julie Kerr-Conte, Francois Pattou,Chunhuan Jin, Yoshikatsu Kanai, Kazuki Sasaki, Naoto Minamino, Hideyuki Sakoda & Masamitsu Nakazato
Nature Communications 14 ( 8158 ) 2023.12
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
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Reversible neuropathic pain model created by long-term optogenetic nociceptor stimulation using light-responsive pain mice Reviewed
Satoshi Kouroki, Toyoaki Maruta, Kotaro Hidaka, Tomohiro Koshida, Mio Kurogi, Yohko Kage, Ayako Miura, Hikaru Nakagawa, Toshihiko Yanagita, Ryu Takeya, Isao Tsuneyoshi
PLOS ONE 2025.4
Language：English Publishing type：Research paper (scientific journal)
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The expression of the formin Fhod3 in mouse tongue striated muscle Reviewed International coauthorship
Nakagawa Hikaru, Kage Yohko, Miura Ayako, Wahyu Sulistomo Hikmawan, Matsuyama Sho, Yamashita Yoshihiro, Takeya Ryu
Cell Structure and Function advpub ( 0 ) 111 - 122 2024.10
Language：English Publishing type：Research paper (scientific journal) Publisher：Japan Society for Cell Biology
The sarcomere is the contractile unit of striated muscle and is composed of actin and myosin filaments. There is increasing evidence to support that actin assembly mediated by Fhod3, a member of the formin family of proteins, is critical for sarcomere formation and maintenance in cardiac muscle. Fhod3, which is abundantly expressed in the heart, localizes to the center of sarcomeres and contributes to the regulation of the cardiac function, as evidenced by the fact that mutations in Fhod3 cause cardiomyopathy. However, the role of Fhod3 in skeletal muscle, another type of striated muscle, is unclear. We herein show that Fhod3 is expressed in the tongue at both mRNA and protein levels, although in smaller amounts than in the heart. To determine the physiological role of Fhod3 expressed in the tongue, we generated embryos lacking Fhod3 in the tongue. The tongue tissue of the Fhod3-depleted embryos did not show any significant structural defects, suggesting that Fhod3 is dispensable for normal development of the mouse tongue. Unexpectedly, the immunostaining analysis revealed the absence of specific sarcomeric signals for Fhod3 in the wild-type tongue when compared to the Fhod3-depleted tongue as a negative control, despite the use of antibodies that had previously been validated by immunostaining of heart tissues. Taken together, although Fhod3 protein is expressed at a significant level in the tongue, Fhod3 in the tongue does not appear to exhibit the same sarcomeric pattern as observed in the heart, suggesting a different role for Fhod3 in the tongue muscles.Key words: actin, formin, sarcomere, striated muscle
DOI： 10.1247/csf.24044
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Maruta T., Kouroki S., Kurogi M., Hidaka K., Koshida T., Miura A., Nakagawa H., Yanagita T., Takeya R., Tsuneyoshi I.
Journal of Neuroscience Research 102 ( 10 ) 2024.10
Publishing type：Research paper (scientific journal) Publisher：Journal of Neuroscience Research
Voltage-gated sodium channels, including NaV1.7, NaV1.8, and NaV1.9, play important roles in pain transmission and chronic pain development. However, the specific mechanisms of their action remain unclear, highlighting the need for in vivo stimulation studies of these channels. Optogenetics, a novel technique for targeting the activation or inhibition of specific neural circuits using light, offers a promising solution. In our previous study, we used optogenetics to selectively excite NaV1.7-expressing neurons in the dorsal root ganglion of mice to induce nocifensive behavior. Here, we further characterize the impact of nocifensive behavior by activation of NaV1.7, NaV1.8, or NaV1.9-expressing neurons. Using CRISPR/Cas9-mediated homologous recombination, NaV1.7–iCre, NaV1.8–iCre, or NaV1.9–iCre mice expressing iCre recombinase under the control of the endogenous NaV1.7, NaV1.8, or NaV1.9 gene promoter were produced. These mice were then bred with channelrhodopsin-2 (ChR2) Cre–reporter Ai32 mice to obtain NaV1.7–ChR2, NaV1.8–ChR2, or NaV1.9–ChR2 mice. Blue light exposure triggered paw withdrawal in all mice, with the strongest response in NaV1.8–ChR2 mice. These light sensitivity differences observed across NaV1.x–ChR2 mice may be dependent on ChR2 expression or reflect the inherent disparities in their pain transmission roles. In conclusion, we have generated noninvasive pain models, with optically activated peripheral nociceptors. We believe that studies using optogenetics will further elucidate the role of sodium channel subtypes in pain transmission.
DOI： 10.1002/jnr.25386
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The NERP-4–SNAT2 axis regulates pancreatic β-cell maintenance and function Reviewed
Zhang W., Miura A., Abu Saleh M.M., Shimizu K., Mita Y., Tanida R., Hirako S., Shioda S., Gmyr V., Kerr-Conte J., Pattou F., Jin C., Kanai Y., Sasaki K., Minamino N., Sakoda H., Nakazato M.
Nature Communications 14 ( 1 ) 2023.12
Publishing type：Research paper (scientific journal) Publisher：Nature Communications
Insulin secretion from pancreatic β cells is regulated by multiple stimuli, including nutrients, hormones, neuronal inputs, and local signalling. Amino acids modulate insulin secretion via amino acid transporters expressed on β cells. The granin protein VGF has dual roles in β cells: regulating secretory granule formation and functioning as a multiple peptide precursor. A VGF-derived peptide, neuroendocrine regulatory peptide-4 (NERP-4), increases Ca2+ influx in the pancreata of transgenic mice expressing apoaequorin, a Ca2+-induced bioluminescent protein complex. NERP-4 enhances glucose-stimulated insulin secretion from isolated human and mouse islets and β-cell–derived MIN6-K8 cells. NERP-4 administration reverses the impairment of β-cell maintenance and function in db/db mice by enhancing mitochondrial function and reducing metabolic stress. NERP-4 acts on sodium-coupled neutral amino acid transporter 2 (SNAT2), thereby increasing glutamine, alanine, and proline uptake into β cells and stimulating insulin secretion. SNAT2 deletion and inhibition abolish the protective effects of NERP-4 on β-cell maintenance. These findings demonstrate a novel autocrine mechanism of β-cell maintenance and function that is mediated by the peptide–amino acid transporter axis.
Books 【 display / non-display 】
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PACAP・VIP受容体の構造と機能の多様性 -PACAPの中枢神経機能を中心として-
宮田篤郎, 三浦綾子（ Role： Joint author）
医学のあゆみ. 医歯薬出版. 第五土曜特集. 2010;233(9),928-33. 2010
Language：Japanese Book type：Scholarly book
Presentations 【 display / non-display 】
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新規ペプチドNERP-4はアミノ酸トランスポーターSNAT2を介して膵β細胞機能を改善する International coauthorship
三浦 綾子、張 維東、迫田 秀之、南野 直人、中里 雅光
第77回日本薬理学会西南部会 2024.11.16
Event date： 2024.11.16
Language：Japanese Presentation type：Oral presentation (general)
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Role of ERM proteins in the regulation of actin cytoskeleton in migrating alveolar macrophage
Ayako Miura，Fumiyuki Sanematsu，Ryu Takeya
2023.12
Event date： 2023.12.14 - 2023.12.16
Language：English Presentation type：Poster presentation
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Regulation of directional cell motility in alveolar macrophages by a formin family protein Fhod1
Ayako Miura，Fumiyuki Sanematsu，Ryu Takeya
2022.11
Event date： 2022.11.30 - 2022.12.3
Language：English Presentation type：Poster presentation
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ARDS線維化過程におけるfibroblast/myofibroblastの核内LOXL2阻害の意義
松尾彩子、谷田亮太、柳 重久、坪内拡伸、三浦綾子、重草貴文、松元信弘、中里雅光、宮崎泰可
第62回日本呼吸器学会学術講演会 2022.4
Event date： 2022.4.22 - 2022.4.24
Language：Japanese Presentation type：Oral presentation (general)
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The actin-nucleating protein Fhod1 in alveolar macrophage
Ayako Miura，Fumiyuki Sanamatsu，Ryu Takeya
2022.3
Event date： 2022.3.7 - 2022.3.9
Language：English Presentation type：Poster presentation
Awards 【 display / non-display 】
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松尾壽之賞
2025.3 宮崎大学 ペプチド研究を基盤とした生体機能制御機構の解析
三浦綾子
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肺がん検診への導入を目指した診断技術の開発に関する臨床疫学研究
2018 2018年度がん研究財団シニアリサーチフェロー
三浦綾子
Award type：Award from Japanese society, conference, symposium, etc.
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ERS Young Scientist Sponsorship
2016.9 Europian Respiratory Society The role of Pten in the cell-fate determination of epithelial cells in lung development.
三浦綾子
Award type：Award from international society, conference, symposium, etc. Country：United Kingdom
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Kyushu Diabetes Research Conference 最優秀奨励賞
2016.7 Kyusyu Diabetes Research Conference 糖代謝調節に機能する新規生理活性ペプチドの発見
三浦綾子
Award type：Award from Japanese society, conference, symposium, etc. Country：Japan
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児玉記念基礎医学助成基金 優秀研究論文顕彰
2013.10 児玉記念基礎医学助成基金 Pituitary adenylate cyclase-activating polypeptide type 1 receptor (PAC1) gene is suppressed by transglutaminase 2 activation.
三浦綾子
Award type：Award from publisher, newspaper, foundation, etc. Country：Japan
Grant-in-Aid for Scientific Research 【 display / non-display 】
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異種細胞間の細胞接着装置の恒常性維持機構の解析
Grant number：21K08183 2021.04 - 2024.03
独立行政法人日本学術振興会 科学研究費補助金 基盤研究(C)
Authorship：Principal investigator
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サルコメアは回転トルクを生み出すか？
Grant number：22K19407 2022.04 - 2024.03
独立行政法人日本学術振興会 科学研究費補助金 挑戦的研究(萌芽)
武谷 立、
Authorship：Coinvestigator(s)
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The Role of Epithelial Pten in Epithelial Cell Fate and Programmed Cellular Senescence during Lung Development
Grant number：17K16051 2017.04 - 2021.03
Grant-in-Aid for Scientific Research Grant-in-Aid for Young Scientists(B)
Authorship：Principal investigator
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上皮間葉連関を焦点とした肺発生での上皮Ptenの機能解析
Grant number：26860610 2014.04 - 2016.03
科学研究費補助金 若手研究(B)
Authorship：Principal investigator
Social Activities 【 display / non-display 】
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令和5年度宮崎サイエンスキャンプ「“くすり”は何故効くの？」
Role(s)： Lecturer, Organizing member
2023.8.7 - 2023.8.8
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日南学園高等学校田野看護専攻科「生物学」講義
Role(s)： Lecturer
2020.6.11 - Now
Audience： High school students
Type：Other