TANAKA Mio

写真a

Affiliation

Faculty of Medicine School of Medicine Department of Anatomy, Histochemistry and Cell Biology

Title

Assistant Professor

External Link

Degree 【 display / non-display

  • Medical Science ( 2021.6   Nagasaki University )

  • Medical Doctor ( 2015.3   Jikei University )

 

Papers 【 display / non-display

  • Crucial role of high-mobility group box 2 in mouse ovarian follicular development through estrogen receptor beta

    Yamaguma Y., Sugita N., Choijookhuu N., Yano K., Lee D., Ikenoue M., Fidya , Shirouzu S., Ishizuka T., Tanaka M., Yamashita Y., Chosa E., Taniguchi N., Hishikawa Y.

    Histochemistry and Cell Biology   157 ( 3 )   359 - 369   2022.3

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    Language:Japanese   Publishing type:Research paper (scientific journal)   Publisher:Histochemistry and Cell Biology  

    High-mobility group box 2 (HMGB2) is a chromatin-associated protein that is an important regulator of gene transcription, recombination, and repair processes. The functional importance of HMGB2 has been reported in various organs, including the testis, heart, and cartilage. However, its role in the ovary is largely unknown. In this study, ovary tissues from wild-type (WT) and HMGB2-knock-out (KO) mice were examined by histopathological staining and immunohistochemistry. The ovary size and weight were significantly lower in HMGB2-KO mice than in age-matched WT littermates. Histopathological analysis revealed ovarian atrophy and progressive fibrosis in 10-month-old HMGB2-KO mouse ovaries. Compared to age-matched WT mice, the numbers of oocytes and developing follicles were significantly decreased at 2 months of age and were completely depleted at 10 months of age in HMGB2-KO mice. Immunohistochemistry revealed the expression of HMGB2 in the granulosa cells of developing follicles, oocytes, some corpora lutea, and stromal cells. Importantly, HMGB2-positive cells were co-localized with estrogen receptor beta (ERβ), but not ERα. Estrogen response element-binding activity was demonstrated by southwestern histochemistry, and it was decreased in HMGB2-KO mouse ovaries. Cell proliferation activity was also decreased in HMGB2-KO mouse ovaries in parallel with the decreased folliculogenesis. These results indicated that the depletion of HMGB2 induced ovarian atrophy that was characterized by a decreased ovarian size and weight, progressive fibrosis, as well as decreased oocytes and folliculogenesis. In conclusion, we demonstrated the crucial role of HMGB2 in mouse ovarian folliculogenesis through ERβ expression.

    DOI: 10.1007/s00418-022-02074-4

    Scopus

    PubMed

  • Correction: Dynamics of serological responses to defined recombinant proteins during Schistosoma mansoni infection in mice before and after the treatment with praziquantel.

    Mohammed ES, Nakamura R, Kalenda YD, Deloer S, Moriyasu T, Tanaka M, Fujii Y, Kaneko S, Hirayama K, Ibrahim AI, El-Seify MA, Metwally AM, Hamano S

    PLoS neglected tropical diseases   16 ( 3 )   e0010309   2022.3

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1371/journal.pntd.0010309

    PubMed

  • Potential of antibody test using Schistosoma mansoni recombinant serpin and RP26 to detect light-intensity infections in endemic areas

    Tanaka M., Kildemoes A.O., Chadeka E.A., Cheruiyot B.N., Sassa M., Moriyasu T., Nakamura R., Kikuchi M., Fujii Y., de Dood C.J., Corstjens P.L.A.M., Kaneko S., Maruyama H., Njenga S.M., de Vrueh R., Hokke C.H., Hamano S.

    Parasitology International   83   102346   2021.8

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    Language:Japanese   Publishing type:Research paper (scientific journal)   Publisher:Parasitology International  

    Schistosomiasis remains a worldwide public health problem, especially in sub-Saharan Africa. The World Health Organization targets the goal for its elimination as a public health problem in the 2030 Neglected Tropical Diseases (NTDs) Roadmap. Concerted action and agile responses to challenges will be necessary to achieve the targets. Better diagnostic tests can accelerate progress towards the elimination by monitoring disease trends and evaluating the effectiveness of interventions; however, current examinations such as Kato–Katz technique are of limited power to detect light-intensity infections. The point-of-care circulating cathodic antigen (POC-CCA) test shows a higher sensitivity compared to the reference standard, Kato-Katz technique, but it still lacks sufficient sensitivity with low infection intensity. In this study, we examined antibody reactions against recombinant protein antigens; Schistosoma mansoni serine protease-inhibitor (SmSerpin) and RP26, by enzyme-linked immunosorbent assay (ELISA) in plasma samples with light-intensity infection. The sensitivity using the cocktail antigen of recombinant SmSerpin and RP26 showed 83.7%. The sensitivity using S. mansoni soluble egg antigen (SmSEA) was 90.8%, but it showed poor specificity (29.7%), while the cocktail antigen presented improved specificity (61.4%). We conclude that antibody detection to the SmSerpin and RP26 protein antigens is effective to detect S. mansoni light-intensity infections. Our study indicates the potential of detecting antibody against recombinant protein antigens to monitor the transmission of schistosomiasis in low endemicity contexts.

    DOI: 10.1016/j.parint.2021.102346

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    PubMed

  • Enzyme-linked immunosorbent assay (ELISA) using recombinant Fasciola cathepsin L1 for the diagnosis of human fasciolosis caused by Fasciola hepatica/gigantica hybrid type

    Sugiyama T., Ichikawa-Seki M., Sato H., Kounosu A., Tanaka M., Maruyama H.

    Parasitology International   82   102311   2021.6

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    Language:Japanese   Publishing type:Research paper (scientific journal)   Publisher:Parasitology International  

    © 2021 The Authors Recombinant Fasciola cathepsin L-1 (rCatL1) was evaluated in enzyme-linked immunosorbent assay (ELISA) for the serodiagnosis of human fasciolosis in Japan. Quality characteristics of the test were accessed by receiver operating characteristic (ROC) analysis, with sera from fasciolosis patients (n = 10), patients with no evidence of parasitic infections (n = 29), and patients with other helminth infections (n = 119). Both the sensitivity and specificity of the test achieved 100% with the control samples. To test the performance of the assay in an authentic situation, 311 serum samples, which had been sent to our laboratory for the diagnosis of parasitic infections from January 2018 to February 2019, were re-assessed using the rCatL1 ELISA. In this case, the sensitivity of the rCatL1 ELISA was 100%, giving positive results to all fasciolosis sera (n = 7), and the specificity was 99.0%, in which three of the 304 non-fasciolosis samples were judged positive. Careful re-examination of the laboratory data and medical imaging of these three patients revealed that one of the patients, who had been diagnosed as having larva migrans syndrome, was judged to be infected with Fasciola, in addition to ascarid nematodes. Thus the true specificity of the assay in the authentic reached 99.3% (302/304). As the rCatL1 ELISA exhibited a highly significant positive likelihood ratio (152.0) and negative likelihood ratio (0.0), calculated from the 311 sample data, this rCatL1 ELISA can be used for routine screening and definitive diagnosis test for fasciolosis in reference laboratories.

    DOI: 10.1016/j.parint.2021.102311

    Scopus

    PubMed

  • Dynamics of serological responses to defined recombinant proteins during Schistosoma mansoni infection in mice before and after the treatment with praziquantel.

    Mohammed ES, Nakamura R, Kalenda YD, Deloer S, Moriyasu T, Tanaka M, Fujii Y, Kaneko S, Hirayama K, Ibrahim AI, El-Seify MA, Metwally AM, Hamano S

    PLoS neglected tropical diseases   14 ( 9 )   e0008518   2020.9

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1371/journal.pntd.0008518

    PubMed

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Grant-in-Aid for Scientific Research 【 display / non-display

  • 寄生虫感染モデルを用いたマウス小腸上皮における細胞間接着制御機構の解析

    Grant number:21K20743  2021.04 - 2024.03

    独立行政法人日本学術振興会  研究活動スタート支援

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    Authorship:Principal investigator