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Affiliation |
Faculty of Medicine School of Medicine Department of Infectious Diseases, Immunology |
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Assistant Professor |
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Related SDGs |
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MITOMA Shuya
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Degree 【 display / non-display 】
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Ph.D. (Veterinary Medicine) ( 2021.3 University of Miyazaki )
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Veterinary Medicine ( 2017.3 University of Miyazaki )
Research Areas 【 display / non-display 】
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Life Science / Veterinary medical science / Immunology
Education 【 display / non-display 】
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University of Miyazaki Interdisciplinary Graduate School of Medicine and Veterinary Medicine
2017.4 - 2021.3
Country：Japan
Papers 【 display / non-display 】
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The detection of long-lasting memory foot-and-mouth disease (FMD) virus serotype O-specific CD4+ T cells from FMD-vaccinated cattle by bovine major histocompatibility complex class II tetramer. Reviewed International coauthorship International journal
Shuya Mitoma, Brigid Veronica Carr, Yongjie Harvey, Katy Moffat, Satoshi Sekiguchi, Bryan Charleston, Junzo Norimine, Julian Seago
Immunology 164 ( 2 ) 266 - 278 2021.10
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
Foot-and-mouth disease (FMD) is a highly contagious, economically devastating disease of cloven-hooved animals. The development of long-lasting effective FMD vaccines would greatly benefit the global FMD control programme. Deep analysis of adaptive immunity in cattle vaccinated against FMD is technically challenging due to the lack of species-specific tools. In this study, we aimed to identify CD4+ T-cell epitopes in the FMD virus (FMDV) capsid and to phenotype the CD4+ T cells that recognize them using bovine major histocompatibility complex (BoLA) class II tetramer. A BoLA class II tetramer based on the DRA/DRB3*020:02 allele and FMDV antigen-stimulated PBMCs from bovine vaccinates were used to successfully identify four epitopes in the FMDV capsid, three of which have not been previously reported; two epitopes were identified in the structural protein VP1, one in VP3 and one in VP4. Specificity of the three novel epitopes was confirmed by proliferation assay. All epitope-expanded T-cell populations produced IFN-γ in vitro, indicating a long-lasting Th1 cell phenotype after FMD vaccination. VP3-specific CD4+ T cells exhibited the highest frequency amongst the identified epitopes, comprising >0·004% of the CD4+ T-cell population. CD45RO+ CCR7+ defined central memory CD4+ T-cell subpopulations were present in higher frequency in FMDV-specific CD4+ T-cell populations from FMD-vaccinated cattle ex vivo. This indicates an important role in maintaining cell adaptive immunity after FMD vaccination. Notably, FMDV epitope-loaded tetramers detected the presence of FMDV-specific CD4+ T cells in bovine PBMC more than four years after vaccination. This work contributes to our understanding of vaccine efficacy.
DOI： 10.1111/imm.13367
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Yuki Tahira, Kotaro Shide, Takuro Kameda, Taisuke Uchida, Ayako Kamiunten, Keiichi Akizuki, Yoko Kubuki, Masayoshi Karasawa, Ryoma Ikeda, Kengo Matsumoto, Jie Bai, Minoru Terashima, Koji Kato, Tomofumi Uto, Tomohiro Fukaya, Shuya Mitoma, Katsuaki Sato, Yudai Uehira, Hiroaki Ueno, Goro Sashida, Hideki Yamaguchi, Kazuya Shimoda
Blood Neoplasia 2 ( 3 ) 100087 - 100087 2025.8
Publishing type：Research paper (scientific journal) Publisher：Elsevier BV
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Selectivity of bovine interleukin-2 mutein stimulation on bovine peripheral blood mononuclear cells Reviewed
MITOMA Shuya, UTO Tomofumi, FUKAYA Tomohiro, TOMINAGA Moe, SEKIGUCHI Satoshi, SATO Katsuaki, NORIMINE Junzo
Journal of Veterinary Medical Science 87 ( 7 ) 781 - 790 2025
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal) Publisher：JAPANESE SOCIETY OF VETERINARY SCIENCE
Delivery of engineered interleukin-2 (IL-2) variants (muteins) is thought to be a promising cancer therapy in humans and mice. Our previous study indicated that bovine IL-2 (boIL-2) has a great potential to elicit NK cell activity for which distribution of IL-2 receptors on the target cell surface influences signal transduction. We developed nine boIL-2 muteins and examined the influence of the muteins on bovine peripheral blood mononuclear cells <i>in vitro</i>. On bovine peripheral mononuclear cells, NK cells strongly expressed CD122, followed by CD8<sup>+</sup> T cells, while CD4<sup>+</sup> T cells and γδ T cells did not show significant CD122 expression. All boIL-2 muteins showed decreasing in binding to boIL-2 receptor α, CD25, while maintaining their ability to bind to boIL-2 receptor βγ, CD122/CD132, heterodimer. The mutein F44A and E63A suppressed CD4<sup>+</sup> T cell expansion but maintained the NK cell expansion. These results indicate that boIL-2 muteins can alter immunological outcomes and may be used for clinical intervention for a disease progression.
DOI： 10.1292/jvms.24-0470
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Lkham-Erdene Baljinnyam, Choijookhuu Narantsog, Kubota Toshiki, Uto Tomofumi, Mitoma Shuya, Shirouzu Shinichiro, Ishizuka Takumi, Kai Kengo, Higuchi Kazuhiro, Mo Aung Kham, Batmunkh Jargal-Erdene, Sato Katsuaki, Hishikawa Yoshitaka
ACTA HISTOCHEMICA ET CYTOCHEMICA 57 ( 5 ) 175 - 188 2024.10
Language：English Publishing type：Research paper (scientific journal) Publisher：JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY
Metabolic dysfunction-associated steatotic liver disease (MASLD) is becoming a major health problem worldwide. Liver regeneration is crucial for restoring liver function, and is regulated by extraordinary complex process, involving numerous factors under both physiologic and pathologic conditions. Sphingosine-1-phosphate (S1P), a bioactive sphingolipid synthesized by sphingosine kinase 1 (SphK1), plays an important role in liver function through S1P receptors (S1PRs)-expressing cells. In this study, we investigated the effect of lipid overload on hepatocyte proliferation in a mouse hepatic steatosis model induced by feeding a methionine- and choline-deficient (MCD) diet. After 50% partial hepatectomy (PHx), liver tissues were sampled at various timepoints and then analyzed by immunohistochemistry, oil Red-O staining, quantitative-polymerase chain reaction (qPCR), and flow cytometry. In mice fed the MCD-diet, significantly exacerbated hepatic steatosis and accelerated liver regeneration were observed. After PHx, hepatocyte proliferation peaked at 48 and 36 hr in the liver of chow- and MCD-diet fed mice, respectively. By contrast, increased expression of S1PR2 was observed in hepatic neutrophils and macrophages of MCD-diet fed mice. Flow cytometry and qPCR experiments demonstrated that levels of HGF and FGF2 released by neutrophils and macrophages were significantly higher in MCD-diet fed mice. In conclusion, hepatic lipid overload recruits Kupffer cells and neutrophils that release HGF and FGF2 via SphK1/S1PR2 activation to accelerate hepatocyte proliferation.
DOI： 10.1267/ahc.24-00046
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Crucial role of dendritic cells in the generation of anti-tumor T-cell responses and immunogenic tumor microenvironment to suppress tumor development. Reviewed International journal
Moe Tominaga, Tomofumi Uto, Tomohiro Fukaya, Shuya Mitoma, Dieter Riethmacher, Kunihiko Umekita, Yoshihiro Yamashita, Katsuaki Sato
Frontiers in immunology 15 1200461 - 1200461 2024
Language：English Publishing type：Research paper (scientific journal)
Dendritic cells (DCs) are known as unique professional antigen (Ag)-presenting cells (APCs) to prime naïve T cells for the initiation of adaptive immunity. While DCs are believed to play a pivotal role in generating anti-tumor T-cell responses, the importance of DCs in the protection from the progression of tumors remains elusive. Here, we show how the constitutive deficiency of CD11chi DCs influences the progression of tumors with the use of binary transgenic mice with constitutive loss of CD11chi DCs. Constitutive loss of CD11chi DCs not only enhances the progression of tumors but also reduces the responses of Ag-specific T cells. Furthermore, the congenital deficiency of CD11chi DCs generates the immunosuppressive tumor microenvironment (TME) that correlates with the marked accumulation of myeloid-derived suppressor cells (MDSCs) and the prominent productions of immunosuppressive mediators. Thus, our findings suggest that CD11chi DCs are crucial for generating anti-tumor T-cell responses and immunogenic TME to suppress the development of tumors.
MISC 【 display / non-display 】
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樹状細胞のはたらき⑧樹状細胞を標的とした免疫チェックポイント阻害療法
宇都倫史, 深谷知宏, 三苫修也, 佐藤克明
Veterinary Immunology for Practitioners ( 37 ) 26 - 31 2025.1
Publishing type：Rapid communication, short report, research note, etc. (scientific journal)
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樹状細胞のはたらき⑦がん免疫応答における通常型樹状細胞の役割
宇都倫史, 深谷知宏, 三苫修也, 佐藤克明
Veterinary Immunology for Practitioners ( 36 ) 46 - 47 2024.10
Publishing type：Rapid communication, short report, research note, etc. (scientific journal)
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宮崎県における牛ウイルス性下痢の積極的疫学調査と分離株の遺伝子系統解析
野津 昂亮, 龍 千香, Ul Hakim Bin Rahmat Luqman, Ullah Shakir, 植木 萌葉, 三苫 修也, 乗峰 潤三, 青木 博史, 亀山 健一郎, 迫田 義博, 関口 敏
獣医疫学雑誌 28 ( 1 ) 20 - 21 2024.7
Language：Japanese Publishing type：Rapid communication, short report, research note, etc. (scientific journal) Publisher：獣医疫学会
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樹状細胞のはたらき⑥アレルギー疾患に対する舌下免疫療法の防御効果における樹状細胞の役割.
深谷知宏, 宇都倫史, 三苫修也, 佐藤克明
Veterinary Immunology for Practitioners ( 35 ) 2024
Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media)
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牛伝染性リンパ腫の抵抗性判別 Invited
関口敏, 三苫修也, 野津昴亮
臨床獣医 42 ( 6 ) 2024
Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media)
Presentations 【 display / non-display 】
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高度免疫不全マウスを用いた牛伝染性リンパ腫担がんマウスモデル及び牛免疫細胞移植マウスモデルの作製と有用性の検討
三苫 修也, 宇都 倫史, 深谷 知宏, 冨永 萌, 関口 敏, 佐藤 克明
第167回日本獣医学会学術集会 2024.9.10
Event date： 2024.9.10 - 2024.9.13
Presentation type：Oral presentation (general)
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ルシフェラーゼ融合蛋白結合抗体捕捉法を用いた抗牛伝染性リンパ腫ウイルス抗体検出法の開発
内田 大紀, 三苫 修也, 野津 昂亮, 関口 敏
第167回日本獣医学会学術集会 2024.9.10
Event date： 2024.9.10 - 2024.9.13
Presentation type：Oral presentation (general)
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Selective cell stimulation with bovine interleukin-2 mutein and the distribution of bovine CD122 on the peripheral lymphocytes
The 52nd Annual Meeting of the Immunology Society of Japan 2024.1.17
Event date： 2024.1.17 - 2024.1.19
Language：English Presentation type：Poster presentation
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外部導入牛における牛白血病ウイルス感染の定量的リスク分析
野津昂亮, WIRATSUDAKUL Anuwat, 三苫修也, 乗峰潤三, 関口敏
日本産業動物獣医学会(九州)・日本小動物獣医学会(九州)・日本獣医公衆衛生学会(九州) 2017
Event date： 2017
Presentation type：Oral presentation (general)
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宮崎県における肉用牛生産者を対象とした牛白血病対策に関する意識調査
森山楓栞, 耕野拓一, 窪田さと子, 三苫修也, 目堅博久, 乗峰潤三, 関口敏
日本産業動物獣医学会(九州)・日本小動物獣医学会(九州)・日本獣医公衆衛生学会(九州) 2017
Event date： 2017
Presentation type：Oral presentation (general)
Grant-in-Aid for Scientific Research 【 display / non-display 】
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The protective immune effect on bovine leukemia virus infection by selective activating the NK cell
Grant number：22K15013 2022.04 - 2025.03
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Early-Career Scientists Grant-in-Aid for Early-Career Scientists
Authorship：Principal investigator