三苫 修也 (ミトマ シュウヤ)

MITOMA Shuya

写真a

所属

医学部 医学科 感染症学講座免疫学分野

職名

助教

外部リンク

学位 【 表示 / 非表示

  • 博士(獣医学) ( 2021年3月   宮崎大学 )

  • 学士(獣医学) ( 2017年3月   宮崎大学 )

研究分野 【 表示 / 非表示

  • ライフサイエンス / 獣医学  / 免疫

学歴 【 表示 / 非表示

  • 宮崎大学   大学院医学獣医学総合研究科(博士課程・修士課程)   医学獣医学専攻(博士課程)

    2017年4月 - 2021年3月

      詳細を見る

    国名:日本国

 

論文 【 表示 / 非表示

  • The detection of long-lasting memory foot-and-mouth disease (FMD) virus serotype O-specific CD4<sup>+</sup> T cells from FMD-vaccinated cattle by bovine major histocompatibility complex class II tetramer 査読あり 国際共著 国際誌

    Mitoma S., Carr B.V., Harvey Y., Moffat K., Sekiguchi S., Charleston B., Norimine J., Seago J.

    Immunology   164 ( 2 )   266 - 278   2021年10月

     詳細を見る

    担当区分:筆頭著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Immunology  

    Foot-and-mouth disease (FMD) is a highly contagious, economically devastating disease of cloven-hooved animals. The development of long-lasting effective FMD vaccines would greatly benefit the global FMD control programme. Deep analysis of adaptive immunity in cattle vaccinated against FMD is technically challenging due to the lack of species-specific tools. In this study, we aimed to identify CD4+ T-cell epitopes in the FMD virus (FMDV) capsid and to phenotype the CD4+ T cells that recognize them using bovine major histocompatibility complex (BoLA) class II tetramer. A BoLA class II tetramer based on the DRA/DRB3*020:02 allele and FMDV antigen-stimulated PBMCs from bovine vaccinates were used to successfully identify four epitopes in the FMDV capsid, three of which have not been previously reported; two epitopes were identified in the structural protein VP1, one in VP3 and one in VP4. Specificity of the three novel epitopes was confirmed by proliferation assay. All epitope-expanded T-cell populations produced IFN-γ in vitro, indicating a long-lasting Th1 cell phenotype after FMD vaccination. VP3-specific CD4+ T cells exhibited the highest frequency amongst the identified epitopes, comprising >0·004% of the CD4+ T-cell population. CD45RO+CCR7+ defined central memory CD4+ T-cell subpopulations were present in higher frequency in FMDV-specific CD4+ T-cell populations from FMD-vaccinated cattle ex vivo. This indicates an important role in maintaining cell adaptive immunity after FMD vaccination. Notably, FMDV epitope-loaded tetramers detected the presence of FMDV-specific CD4+ T cells in bovine PBMC more than four years after vaccination. This work contributes to our understanding of vaccine efficacy.

    DOI: 10.1111/imm.13367

    Scopus

    PubMed

  • Identifying Pathogen and Allele Type Simultaneously in a Single Well Using Droplet Digital PCR. 査読あり 国際誌

    Kosuke Notsu, Hala El Daous, Shuya Mitoma, Xinyue Wu, Junzo Norimine, Satoshi Sekiguchi

    mSphere   e0049322   2023年1月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    In the transmission control of chronic and untreatable livestock diseases such as bovine leukemia virus (BLV) infection, the removal of viral superspreaders is a fundamental approach. On the other hand, selective breeding of cattle with BLV-resistant capacity is also critical for reducing the viral damage to productivity by keeping infected cattle. To provide a way of measuring BLV proviral load (PVL) and identifying susceptible/resistant cattle simply and rapidly, we developed a fourplex droplet digital PCR method targeting the BLV pol gene, BLV-susceptible bovine major histocompatibility complex (BoLA)-DRB3*016:01 allele, resistant DRB3*009:02 allele, and housekeeping RPP30 gene (IPATS-BLV). IPATS-BLV successfully measured the percentage of BLV-infected cells and determined allele types precisely. Furthermore, it discriminated homozygous from heterozygous carriers. Using this method to determine the impact of carrying these alleles on the BLV PVL, we found DRB3*009:02-carrying cattle could suppress the PVL to a low or undetectable level, even with the presence of a susceptible heterozygous allele. Although the population of DRB3*016:01-carrying cattle showed significantly higher PVLs compared with cattle carrying other alleles, their individual PVLs were highly variable. Because of the simplicity and speed of this single-well assay, our method has the potential of being a suitable platform for the combined diagnosis of pathogen level and host biomarkers in other infectious diseases satisfying the two following characteristics of disease outcomes: (i) pathogen level acts as a critical maker of disease progression; and (ii) impactful disease-related host genetic biomarkers are already identified. IMPORTANCE While pathogen-level quantification is an important diagnostic of disease severity and transmissibility, disease-related host biomarkers are also useful in predicting outcomes in infectious diseases. In this study, we demonstrate that combined proviral load (PVL) and host biomarker diagnostics can be used to detect bovine leukemia virus (BLV) infection, which has a negative economic impact on the cattle industry. We developed a fourplex droplet digital PCR assay for PVL of BLV and susceptible and resistant host genes named IPATS-BLV. IPATS-BLV has inherent merits in measuring PVL and identifying susceptible and resistant cattle with superior simplicity and speed because of a single-well assay. Our new laboratory technique contributes to strengthening risk-based herd management used to control within-herd BLV transmission. Furthermore, this assay design potentially improves the diagnostics of other infectious diseases by combining the pathogen level and disease-related host genetic biomarker to predict disease outcomes.

    DOI: 10.1128/msphere.00493-22

    PubMed

  • Virological characteristics of the SARS-CoV-2 Omicron BA.2 subvariants, including BA.4 and BA.5 査読あり 国際誌

    Kimura I., Yamasoba D., Tamura T., Nao N., Suzuki T., Oda Y., Mitoma S., Ito J., Nasser H., Zahradnik J., Uriu K., Fujita S., Kosugi Y., Wang L., Tsuda M., Kishimoto M., Ito H., Suzuki R., Shimizu R., Begum M.M., Yoshimatsu K., Kimura K.T., Sasaki J., Sasaki-Tabata K., Yamamoto Y., Nagamoto T., Kanamune J., Kobiyama K., Asakura H., Nagashima M., Sadamasu K., Yoshimura K., Shirakawa K., Takaori-Kondo A., Kuramochi J., Schreiber G., Ishii K.J., Hashiguchi T., Ikeda T., Saito A., Fukuhara T., Tanaka S., Matsuno K., Sato K.

    Cell   185 ( 21 )   3992 - 4007   2022年10月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Cell  

    After the global spread of the SARS-CoV-2 Omicron BA.2, some BA.2 subvariants, including BA.2.9.1, BA.2.11, BA.2.12.1, BA.4, and BA.5, emerged in multiple countries. Our statistical analysis showed that the effective reproduction numbers of these BA.2 subvariants are greater than that of the original BA.2. Neutralization experiments revealed that the immunity induced by BA.1/2 infections is less effective against BA.4/5. Cell culture experiments showed that BA.2.12.1 and BA.4/5 replicate more efficiently in human alveolar epithelial cells than BA.2, and particularly, BA.4/5 is more fusogenic than BA.2. We further provided the structure of the BA.4/5 spike receptor-binding domain that binds to human ACE2 and considered how the substitutions in the BA.4/5 spike play roles in ACE2 binding and immune evasion. Moreover, experiments using hamsters suggested that BA.4/5 is more pathogenic than BA.2. Our multiscale investigations suggest that the risk of BA.2 subvariants, particularly BA.4/5, to global health is greater than that of original BA.2.

    DOI: 10.1016/j.cell.2022.09.018

    Scopus

    PubMed

  • A pooled testing system to rapidly identify cattle carrying the elite controller BoLA-DRB3*009:02 haplotype against bovine leukemia virus infection 査読あり 国際誌

    Notsu K., El Daous H., Mitoma S., Norimine J., Sekiguchi S.

    HLA   99 ( 1 )   12 - 24   2022年1月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:HLA  

    As genetically resistant individuals, the “elite controllers” (ECs) of human immunodeficiency virus infection have been focused on as the keys to developing further functional treatments in medicine. In the livestock production field, identifying the ECs of bovine leukemia virus (BLV) infection in cattle is desired to stop BLV transmission chains on farms. Cattle carrying the bovine leukocyte antigen (BoLA)-DRB3*009:02 allele (DRB3*009:02) have a strong possibility of being BLV ECs. Most of cattle carrying this allele maintain undetectable BLV proviral loads and do not shed virus even when infected. BLV ECs can act as transmission barriers when placed between uninfected and infected cattle in a barn. To identify cattle carrying DRB3*009:02 in large populations more easily, we developed a pooled testing system. It employs a highly sensitive, specific real-time PCR assay and TaqMan MGB probes (DRB3*009:02-TaqMan assay). Using this system, we determined the percentage of DRB3*009:02-carrying cattle on Kyushu Island, Japan. Our pooled testing system detected cattle carrying the DRB3*009:02 allele from a DNA pool containing one DRB3*009:02-positive animal and 29 cattle with other alleles. Its capacity is sufficient for herd-level screening for DRB3*009:02-carrying cattle. The DRB3*009:02-TaqMan assay showed high-discriminative sensitivity and specificity toward DRB3*009:02, making it suitable for identifying DRB3*009:02-carrying cattle in post-screening tests on individuals. We determined that the percentage of DRB3*009:02-carrying cattle in Kyushu Island was 10.56%. With its ease of use and reliable detection, this new method strengthens the laboratory typing for DRB3*009:02-carrying cattle. Thus, our findings support the use of BLV ECs in the field.

    DOI: 10.1111/tan.14502

    Scopus

    PubMed

  • A survey of bovine leukemia virus resistant bovine leukocyte antigen (BoLA)-DRB3*009:02 allele-carrying Japanese Black cattle in two prefectures in Japan 査読あり

    Notsu K., Inoue Y., Masuda T., Inoue M., Mitoma S., Wu X., Norimine J., Sekiguchi S.

    The Journal of Veterinary Medical Science   84 ( 11 )   1457 - 1460   2022年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:公益社団法人 日本獣医学会  

    The bovine leukocyte antigen (BoLA) DRB3*009:02 allele is strongly associated with a low/undetectable bovine leukemia virus (BLV) proviral load. Understanding the status of cattle possessing DRB3*009:02 allele is key for BLV control by breeding. We performed a survey of DRB3*009:02-carrying cattle in two prefectures in Japan using a TaqMan assay developed previously. The allele was found in 3.8% (confidence interval (CI): 3.3–4.3) of 6020 Japanese Black female cattle. A prefecture-level difference was found: the allele was observed in 8.6% CI: 7.5–9.9) of 2242 cattle of the birth prefecture B in Kyushu/Okinawa region, and this percentage was significantly higher than those of prefecture C in Kyushu/Okinawa region (1.3% (CI: 0.4–3.4) of 319) and prefecture A in Chugoku region (0.9% (CI: 0.6–1.4) of 2741), respectively. Consideration on the difference in possession of DRB3*009:02 allele is needed to establish the more efficient control strategy of BLV infection in Japanese Black cattle.

    DOI: 10.1292/jvms.22-0285

    Scopus

    PubMed

    CiNii Research

全件表示 >>

MISC 【 表示 / 非表示

  • 決定論的手法を用いた肉用牛の外部導入における牛伝染性リンパ腫ウイルス感染のリスク評価

    藤原未歩, 牛谷雄一, 野津昂亮, EL-DAOUS Hala, 芹田光玲, 三苫修也, 乗峰潤三, 乗峰潤三, 関口敏, 関口敏

    獣医疫学雑誌   25 ( 1 )   2021年

     詳細を見る

    掲載種別:速報,短報,研究ノート等(学術雑誌)  

    J-GLOBAL

科研費(文科省・学振・厚労省)獲得実績 【 表示 / 非表示

  • 牛伝染性リンパ腫ウイルス感染症に対するNK細胞選択的活性による防御免疫効果

    研究課題/領域番号:22K15013  2022年04月 - 2025年03月

    日本学術振興会  科学研究費助成事業 若手研究  若手研究

    三苫 修也

     詳細を見る

    担当区分:研究代表者