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Affiliation |
Faculty of Agriculture Region of Veterinary Science |
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Title |
Assistant Professor |
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Contact information |
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Related SDGs |
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FUKE Naoyuki
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Degree 【 display / non-display 】
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Ph.D（Veterinary Medicine） ( 2020.4 University of Miyazaki )
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Veterinary Medicine ( 2016.3 University of Miyazaki )
Research Areas 【 display / non-display 】
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Life Science / Veterinary medical science
Papers 【 display / non-display 】
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KATO Nanami X, DOSAKA Haruka, ADACHI Yukinobu, KANEKO Yasuyuki, HIRAI Takuya, SEKIGUCHI Satoshi, FUKE Naoyuki, SAITO Akatsuki
Journal of Veterinary Medical Science 88 ( 6 ) 838 - 847 2026.6
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：JAPANESE SOCIETY OF VETERINARY SCIENCE
Domestic cat hepatitis B virus (DCHBV) infections are associated with chronic hepatitis and hepatic cancer in cats, similar to human HBV. We previously demonstrated that the positivity rate for DCHBV in Japan is less than 1% among tested samples, suggesting lower viral dissemination in Japan than in other countries. Furthermore, the transmission dynamics of DCHBV remain poorly understood. In this study, we document the first recorded outbreak of DCHBV in a cat shelter in Japan, which had an unprecedentedly high prevalence of 26.59% (46/173 cats). We investigated potential transmission routes via quantitative polymerase chain reaction analysis of matched blood, oral, and rectal swab samples. Blood and oral viral loads were significantly correlated, with viral DNA detected in both oral (40.54%) and rectal (19.44%) cavities of viremic cats. Phylogenetic analysis of the polymerase gene retrieved from deceased cats yielded >99.2% sequence identity, indicating the circulation of a single strain. Pathological assessments, including histopathology, immunohistochemistry, and RNA <i>in situ</i> hybridization, confirmed viral presence in hepatocytes and biliary epithelium, linking the outbreak with severe hepatic lesions, including cholangitis, fibrosis, and cholangiocarcinoma. Our findings indicate that the transmission route of DCHBV may be more complex than initially anticipated, underscoring the importance of understanding the mode of transmission to effectively control DCHBV infection, particularly in multi-cat facilities.
DOI： 10.1292/jvms.26-0138
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ABE Hinata, KATO Nanami X, TANABE Mika, SHIMABUKURO Taichi, HASHIMOTO Saki, HIRAI Takuya, FUKE Naoyuki, SAITO Akatsuki
Journal of Veterinary Medical Science 88 ( 5 ) 710 - 718 2026.6
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：JAPANESE SOCIETY OF VETERINARY SCIENCE
A 12-year-8-month-old neutered male cat presented with multiple nail bed masses and an ulcerative lip lesion. PCR identified <i>Felis catus</i> papillomavirus type 3 (FcaPV3) in the nail masses and type 4 (FcaPV4) in the lip lesion. Histopathology revealed basosquamous cell carcinoma (BSCC) in the digits and Bowenoid <i>in situ</i> carcinoma (BISC) in the lip. Immunohistochemistry showed that tumor cells in both BSCC and BISC were positive for p16 and p63 and weakly positive for pRb; p53 was negative in BSCC but weakly positive in BISC. <i>In situ</i> hybridization confirmed FcaPV3 and FcaPV4 <i>E6</i> genes in digital and lip tumors, respectively. Over 6 months, viral sequencing showed 23 nucleotide changes in FcaPV3, suggesting viral evolution. This is the first report of distinct malignant epithelial tumors independently induced by FcaPV3 and FcaPV4 in a cat.
DOI： 10.1292/jvms.25-0517
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Distribution of domestic cat hepatitis B virus in cholangiocarcinoma and non-neoplastic liver tissue
DOSAKA Haruka, KATO Nanami X, TAGA Kei, YAMAMOTO Soshin, KONDO Kaito, RINGO Rissar Siringo, KANEKO Yasuyuki, HIRAI Takuya, SAITO Akatsuki, FUKE Naoyuki
Journal of Veterinary Medical Science 88 ( 3 ) 458 - 462 2026.3
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：JAPANESE SOCIETY OF VETERINARY SCIENCE
Domestic cat hepatitis B virus (DCHBV), shares similarities with human hepatitis B virus (HBV) which is associated with liver disease. We report the first case of cholangiocarcinoma in a 17-year-old spayed female cat infected with DCHBV and positive for feline immunodeficiency virus. The patient presented with vomiting, anorexia, and an elevated globulin level. Ultrasonography revealed multiple hypoechoic hepatic lesions, and histopathology confirmed cholangiocarcinoma. The tumor exhibited CK7 positivity and HepPar-1 negativity, confirming biliary origin. Quantitative PCR detected DCHBV in the spleen and ascitic fluid, while immunohistochemistry and RNA <i>in situ</i> hybridization revealed viral antigen and mRNA in both tumor and non-tumor liver. The presence of a viral antigen and mRNA in neoplastic tissue suggests a potential role for DCHBV in hepatobiliary carcinogenesis.
DOI： 10.1292/jvms.25-0372
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Generation of anti-bovine CD20 monoclonal antibody with cytotoxic activity for the depletion of bovine B-cell lymphoma cells. Reviewed
Mitoma S, Uto T, Fukaya T, Yamanokuchi N, Sato Y, Nishikawa Y, Wakitani S, Fuke N, Kitahara G, Sekiguchi S, Yasuda M, Sato K
Scientific reports 2026.6
Language：English Publishing type：Research paper (scientific journal)
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Yamashita T., Hegazy A.A., Yamagishi A., Saito E., Kakiya M., Fuke N., Hirai T.
Journal of Comparative Pathology 227 24 - 30 2026.6
Language：English Publishing type：Research paper (scientific journal) Publisher：Journal of Comparative Pathology
Porcine circovirus-associated disease (PCVAD) and porcine respiratory disease complex (PRDC) significantly impact the pig industry worldwide. Porcine circovirus type 2 (PCV2) is the primary pathogen associated with PCVAD, whereas PRDC is caused by a combination of pathogens including porcine reproductive and respiratory syndrome virus (PRRSV) and pathogenic bacteria. Despite the availability of a PCV2 vaccine that effectively controls and prevents PCVAD, subclinical PCV2 infection (PCV2-SI) still occurs under field conditions. Various porcine pathogens enter via the nasal cavity, where nasopharynx-associated lymphoid tissue (NALT) plays an important role in nasal mucosal immunity. This study examined the pathogenesis of PRDC in pigs with PCV2-SI and the distribution of PCV2 and PRRSV in NALT using in-situ hybridization (ISH) analyses in PCV2-SI pigs. All six pigs had suppurative nasopharyngitis and suppurative pneumonia. Lymphoid tissue contained no cytoplasmic inclusions or giant cells. ISH showed positive signals for PCV2 in the NALT and lungs of all six pigs and PRRSV in the NALT of three pigs and lungs of five. PCV2-positive macrophages were primarily identified in the follicles of NALT, whereas PRRSV-positive macrophages were found primarily around crypts. This is the first study to suggest that replication of both viruses in NALT may cause suppression of local immunity and activate important risk factors of PRDC in pigs with PCV2-SI.
Books 【 display / non-display 】
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牛枝肉の”シミ”発生率に影響する生体、飼育管理、およびと畜処理要因の検証
遠矢宏美、福家直幸、萩平敦朗（ Role： Joint author）
畜産技術 2024.10
Language：Japanese Book type：Report
Presentations 【 display / non-display 】
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ネコにおけるSFTSの病理学的特徴-宮崎県の野外感例を中心に- Invited
福家直幸
第168回日本獣医学会学術集会病理分科会 2025.9.4
Event date： 2025.9.3 - 2025.9.5
Language：Japanese Presentation type：Symposium, workshop panel (nominated)
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猫B型肝炎ウイルス（DCHBV）感染猫10例の肝病変に関する病理組織学的検討
銅坂悠、加藤七海、足立行庸、金子泰之、平井卓哉、齊藤暁、福家直幸
第13回日本獣医病理学専門家協会学術集会 2026.3.26
Event date： 2026.3.25 - 2026.3.27
Language：Japanese Presentation type：Poster presentation
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Histopathological analysis of 11 cats infected with severe fever with thrombocytopenia syndrome virus at the Miyazaki prefecture
Muhammad Haroon Khan, Naoyuki Fuke, Ren Takada, Yasuyuki Kaneko, Hirohisa Mekata, Tamaki Okabayashi, Takuya Hirai
第13回日本獣医病理学専門家協会学術集会 2026.3.26
Event date： 2026.3.25 - 2026.3.27
Language：English Presentation type：Poster presentation
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Severe Fever with Thrombocytopenia Syndrome: An Emerging Tick-borne Threat in Asia Invited International conference
Naoyuki Fuke
International Veterinary Conference 2025 2025.11.25
Event date： 2025.11.24 - 2025.11.25
Language：English Presentation type：Oral presentation (invited, special)
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猫ヘパドナウイルス感染を伴う猫の胆管癌
銅坂 悠、加藤 七海、近藤 海斗、多賀 佳 、山本 宗伸、 Rissar Siringo Ringo、金子 泰之、平井 卓哉、齊藤 暁、福家 直幸
令和7年度獣医学術九州地区学会 日本小動物獣医学会 2025.10.26
Event date： 2025.10.26
Language：Japanese Presentation type：Oral presentation (general)
Awards 【 display / non-display 】
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フレッシャー・アワード
2025.10 九州地区獣医師会連合会 猫ヘパドナウイルス感染を伴う猫の胆管癌
銅坂悠、加藤七海、近藤海斗、多賀佳、山本宗伸、Rissar Siringo Ringo、金子泰之、平井卓哉、齊藤暁、福家直幸
Award type：Award from Japanese society, conference, symposium, etc.
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優秀ポスター賞
2024.3 日本獣医病理学専門家協会 宮崎県内で重症熱性血小板減少症を発症した自然感染猫８例における病理学的検索
高田蓮栞、福家直幸、岡林環樹、Mazimpaka Eugene、平井卓哉
Award type：Award from Japanese society, conference, symposium, etc.
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奨励賞
2025.10 九州地区獣医師会連合会 肉用雄子牛の免疫学的去勢法の検討
西岡快人、三樹孝太郎、福家直幸、大澤健司、北原豪
Award type：Award from Japanese society, conference, symposium, etc.
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九州地区獣医師会連合会長賞
2024.9 九州地区獣医師会連合会 豚呼吸器複合病における扁桃と鼻咽頭関連リンパ組織病変の比較検討
齋藤江連、垣屋美穂、坂本英午、福家直幸、平井卓哉
Award type：Award from Japanese society, conference, symposium, etc.
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優秀ポスター賞
2024.3 日本獣医病理学専門家協会 PCV2不顕性感染豚の鼻咽頭関連リンパ組織におけるISH法によるPCV2およびPRRSVの検出
齋藤江連、山下鉄平、坂本英午、福家直幸、平井卓哉
Award type：Award from Japanese society, conference, symposium, etc.
Available Technology 【 display / non-display 】
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Home Page： 宮崎大学 獣医病理学研究室
Related fields where technical consultation is available：・食肉衛生検査所や家畜保健衛生所の診断の助言
・SFTSの病理診断
・ウイルス性疾患（猫パピローマ、猫ヘパドナ）の病理診断
・伴侶動物・産業動物・動物園動物の死因解析（不審死などを含む）Message：獣医病理に関連する内容であれば、御相談下さい。
