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医学部 医学科 臨床神経科学講座脳神経外科学分野 |
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Accurate classification of ependymomas and medulloblastomas using Raman spectroscopy and pilot transcriptomic profiling. 査読あり 国際誌
Kawamoto Y, Okita Y, Temma K, Kubo T, Kumamoto Y, Fujii Y, Nishimoto K, Utsugi R, Yokota C, Hirayama R, Kijima N, Ming KH, Tani N, Oshino S, Kagawa N, Motooka D, Fujita K, Kishima H
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy 352 127532 - 127532 2026年1月
担当区分：責任著者 記述言語：英語 掲載種別：研究論文（学術雑誌）
Raman spectroscopy enabled accurate discrimination of posterior fossa ependymomas and medulloblastomas in both frozen and formalin-fixed, paraffin-embedded (FFPE) specimens in this retrospective study. We acquired Raman spectra (532 nm excitation) from frozen and FFPE tissues to evaluate a principal component analysis-support vector machine classifier by using fivefold cross-validation. We also performed a pilot spatial transcriptomics analysis on three FFPE sections by using the 10× Genomics Xenium In Situ v2 FFPE workflow. In total, 34 specimens (21 frozen and 13 FFPE) were analyzed, and the classification models achieved >90% accuracy in distinguishing ependymomas from medulloblastomas under spectrum-level fivefold cross-validation, suggesting discriminative biochemical differences, whereas patient-level performance requires further validation in larger cohorts. Ependymomas had higher lipid-associated Raman bands (1084, 1128, and 1654 cm-1), whereas medulloblastomas exhibited higher deoxyhemoglobin-related Raman bands (1356, 1548, and 1604 cm-1). Compared with normal controls, tumor tissues had increased carotenoid-related Raman bands and reduced lipid-associated Raman bands. The observed spectral differences are consistent with differences in lipid-associated composition and the heme/oxygenation-related tissue context, and pilot transcriptomic profiling provided qualitative biological context related to lipid metabolism and angiogenesis. These findings support Raman spectroscopy as a label-free spectroscopic technique that may complement conventional diagnostics and aid surgical and therapeutic decision-making. Larger, prospective studies are warranted to further evaluate the clinical generalizability and intraoperative translation of our results.
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A Rare Case of a Solid Variant Aneurysmal Bone Cyst of the Medial Sphenoid Bone: Clinical Features, Diagnostic Points, and Treatment 査読あり
YAMASHITA Shinji, MATSUMOTO Fumitaka, OKUYAMA Hironobu, OGASAWARA Natsuki, TAMURA Mitsuru, KAWANO Tomoki, YOKOGAMI Kiyotaka, KIWAKI Takumi, FUKUSHIMA Tsuyoshi, SATO Yuichiro, TOMONAGA Takumi, OKITA Yoshiko
NMC Case Report Journal 12 ( 0 ) 369 - 375 2025年12月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：一般社団法人 日本脳神経外科学会
A 5-year-old boy presented to our hospital with ptosis and an abnormal ocular position. Magnetic resonance imaging showed a well-defined mass measuring 20 mm in diameter in the medial sphenoid bone extending to the orbit and compressing the external ocular muscle. The patient underwent total surgical excision and was subsequently diagnosed with a solid variant of aneurysmal bone cyst via molecular integrated diagnosis. Solid variant of aneurysmal bone cyst is an extremely rare subtype of aneurysmal bone cyst, accounting for 0.2% of all primary bone tumors. It is characterized by the absence of a solid cystic component, which is difficult to diagnose via conventional hematoxylin and eosin staining. Molecular analyses revealed that this subtype is also characterized by the rearrangement of <i>USP6</i> and the absence of the H3F3A mutation. This report discusses the clinical features of this extremely rare neoplastic lesion, the importance of an integrated diagnosis, and treatment options.
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Sanada T., Shimizu T., Okita Y., Arita H., Sato H., Saito M., Mitsui N., Hiroshima S., Isohashi K., Tanino M., Kanemura Y., Kishima H., Kinoshita M.
Bioengineering 13 ( 1 ) 2025年12月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Bioengineering
This study explored radiomic features that help identify non-contrast-enhancing tumors (nCET) by analyzing regions where contrast-enhancing tumors (CET) transformed into nCET after Bevacizumab (BEV) treatment. The BEV cohort included 24 recurrent GBM (rGBM) patients treated with BEV, showing reduced contrast-enhancement on gadolinium-enhanced T1-weighted imaging (T1Gd) imaging. The 11C-methionine positron emission tomography (Met-PET) cohort consisted of 24 newly diagnosed GBM (nGBM) patients with available Met-PET data. VOIs were created from T2WI, FLAIR, T1Gd, and Met-PET to analyze nCET and T2/FLAIR lesions. After significant radiomic features were identified, a prediction model for nCET was developed in the BEV cohort and subsequently evaluated in the Met-PET cohort. A total of 37 and 46 significant radiomic features were found in the BEV and Met-PET cohorts, respectively. The key feature, T2WI_whole_GLCMcorrelation_1, was selected for predictive modeling. The model demonstrated high accuracy (AUC = 0.93, p < 0.0001) in the BEV cohort, with sensitivity and specificity of 0.91, while the Met-PET cohort showed moderate accuracy (AUC = 0.74, p = 0.0053). Image reconstruction using these features also effectively visualized nCET in nGBM. These findings suggest that radiomic features in CET regions transforming to nCET after BEV treatment harbors valuable information for identifying nCET in GBM.
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Arita H., Ikawa T., Kanayama N., Morimoto M., Umehara T., Yoshizawa H., Kodama Y., Okita Y., Kinoshita M., Konishi K.
Acta Neurochirurgica 167 ( 1 ) 163 2025年12月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Acta Neurochirurgica
Background: Recent advances in cancer treatment have prolonged survival after the onset of brain metastasis (BM), increasing the incidence of local progression (LP) following radiotherapy. However, no standard approach exists for managing LP. We aimed to evaluate the outcomes of salvage surgery in a clinical setting. Methods: The clinical data were retrospectively collected from the medical records of 49 patients who underwent their first salvage surgery for LP of BM at a single institution between April 2014 and March 2024. Overall survival (OS) and LP-free survival (LPFS) were evaluated using the Kaplan–Meier method. Results: Most patients (47/49, 96%) had a history of stereotactic radiosurgery (n = 34) and/or stereotactic radiotherapy (n = 14). The histopathological examination of surgical specimens confirmed tumor recurrence in 33 patients and radiation necrosis (RN) in 16 patients. The interval from prior radiotherapy to salvage surgery was longer in patients with RN than in those with recurrence (median: 42.3 vs. 9.3 months, respectively). OS was longer in the RN group compared with the recurrent group (median: 68.5 months and 21.8 months, respectively). In the recurrent group, shorter OS was associated with preoperative poor KPS (< 70), the presence of active extracranial lesions, and RPA classes 2–3. The extent of resection, postoperative chemotherapy, and local irradiation had no significant effect on OS. After salvage surgery, further LP was observed in 20 patients (61%), with a median LPFS of 7.0 months in the recurrent group. No significant association was found between LPFS and the extent of tumor removal, postoperative chemotherapy, and RT. Conclusions: This study highlights a relatively prolonged survival period following salvage surgery for local progression of BM after irradiation. Salvage surgery is a treatment option in patients with good extracranial control and performance status. The high recurrence rate following salvage treatment underscores the need for developing additional treatment approaches.
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Dysembryoplastic neuroepithelial tumor in an atypical location without epilepsy: a case report 査読あり
Masato Hidaka, Shinji Yamashita, Tsuyoshi Fukushima, Natsuki Ogasawara, Mitsuru Tamura, Tomoki Kawano, Fumitaka Matsumoto, Hironobu Okuyama, Nei Fukasawa, Junko Hirato, Yuichiro Sato, Yoshiko Okita
Acta Scientific Neurology 8 ( 5 ) 89 - 94 2025年4月
担当区分：最終著者 記述言語：英語 掲載種別：研究論文（学術雑誌）
科研費（文科省・学振・厚労省）獲得実績 【 表示 ／ 非表示 】
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膠芽腫に対する新規治療法の探索を可能とするデジタルツインの基盤技術開発
研究課題/領域番号：25K22913 2025年04月 - 2027年03月
独立行政法人日本学術振興会 科学研究費基金 挑戦的研究（萌芽）
担当区分：研究分担者
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膠芽腫におけるシングルセルラマン分光法の確立による腫瘍細胞特性の解明
研究課題/領域番号：24K12261 2024年04月 - 2027年03月
独立行政法人日本学術振興会 科学研究費基金 基盤研究(C)
担当区分：研究代表者
寄附金・講座・研究部門 【 表示 ／ 非表示 】
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臨床神経科学講座脳神経外科学分野研究奨学金
2025年01月
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臨床神経科学講座脳神経外科学分野研究奨学金
2024年11月
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臨床神経科学講座脳神経外科学分野研究奨学金（潤和ﾘﾊﾋﾞﾘﾃｰｼｮﾝ振興財団）
2024年09月
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臨床神経科学講座脳神経外科学分野研究奨学金
2024年06月
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臨床神経科学講座脳神経外科学分野研究奨学金（潤和ﾘﾊﾋﾞﾘﾃｰｼｮﾝ振興財団）
2024年05月