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A Mini-Fluid Challenge to Predict Fluid Responsiveness in Swine 査読あり
Yoshida K.
Journal of the American Association for Laboratory Animal Science 64 ( 1 ) 106 - 110 2025年1月
担当区分：筆頭著者, 最終著者, 責任著者 記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Journal of the American Association for Laboratory Animal Science
Unnecessary and excessive fluid therapy increases the risk of adverse effects such as pulmonary edema. To prevent this, a mini-fluid challenge (MFC) has been utilized to predict whether fluid therapy will improve circulatory dynamics in human intensive care medicine. The study described here investigated whether MFC is also efficacious in pigs. Thirty-two domestic pigs anesthetized and maintained under mechanical ventilation were treated with successive IV fluid administrations of 2, 1, 1, and 2 mL/kg over a 10-min period for a total dose of 6 mL/kg of Ringer lactate. The percentage increase in mean arterial pressure (MAP) at 2, 3, and 4 mL/kg of cumulative fluid administration was examined to determine whether responders could be identified that would benefit hemodynamically from higher doses of fluids. For the purposes of this study, a 10% increase or more in MAP after 6 mL/kg of fluid administration defined responders, and an increase of less than 10% in MAP was used to define nonresponders. The percentage increase in MAP at 2, 3, and 4 mL/kg fluid administration was evaluated to determine whether this could predict responder status. Eleven of the 32 animals were determined to be responders. Responder status was predicted with high accuracy by the administration of 3 mL/kg (AUC = 0.98) and was moderately predicted with administration of 2 mL/kg (AUC = 0.80), as well as pulse pressure variation (AUC = 0.75). Thus, MFC may be helpful to maintain tissue perfusion in pigs through the use of managed fluid therapy.
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Yoshida K.
Journal of the American Association for Laboratory Animal Science 62 ( 2 ) 185 - 188 2023年3月
担当区分：筆頭著者, 最終著者, 責任著者 掲載種別：研究論文（学術雑誌） 出版者・発行元：Journal of the American Association for Laboratory Animal Science
Barbiturate overdose is a common method for euthanizing pigs. However, barbiturates can cause tissue damage and may affect experimental results, so the minimal dose should be used. The minimal dose of barbiturate for euthanasia in pigs under isoflurane anesthesia has not yet been determined. In this study, we compared the effect of low and high doses of 2 barbiturates (pentobarbital, 30 or 60 mg/kg; thiopental, 20 and 40 mg/kg) on hemodynamic parameters and time to cardiac arrest in female pigs maintained on isoflurane. Acute decreases in blood pressure and end-tidal CO<inf>2</inf> occurred in all pigs shortly after administration of the barbiturate. However, these changes were not different between either of the high- and low dose groups. Cardiac arrest occurred significantly faster for high dose as compared with low dose thiopental groups, but this parameter was different between the 2 pentobarbital groups. The bispectral index fell immediately after dosing, in all pigs, but no significant differences were observed in the time needed to achieve 0 for the high or low-doses of either drug. In pigs maintained on isoflurane, a low dose of barbiturates is adequate for euthanasia and may result in less tissue damage.
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Anaphylactoid reaction to dextran 40 during general anesthesia in two domestic pigs 査読あり
Yoshida K.
Laboratory Animals 55 ( 6 ) 568 - 572 2021年12月
担当区分：筆頭著者, 最終著者, 責任著者 記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Laboratory Animals
This report describes two cases of dextran-induced anaphylactoid reaction (DIAR), with and without cutaneous signs that appeared after administration of dextran 40 during general anesthesia in domestic pigs. Dextran was administered intravenously to 59 pigs that underwent general anesthesia for routine medical device training, and two pigs developed sudden, severe hypotension and decreased end-tidal CO<inf>2</inf> level and arterial oxygen saturation within a few minutes after the start of dextran 40 infusion. A systemic cutaneous lesion presenting as flushing and urticaria was observed in case 1, whereas no skin lesions were found despite persistent observation in case 2. Cases 1 and 2 recovered with thoracic wall compression and an intravenous injection of epinephrine (0.02 mg) and hydrocortisone sodium succinate (250 mg). After DIAR treatment, no complications were observed, and the pigs were euthanized with an intravenous injection of thiopental sodium (1.0 g) when the training program was completed. This case report discusses the appearance of DIAR after dextran 40 administration during general anesthesia in domestic pigs, which is similar to DIAR in humans. However, the incidence of DIAR in this study was much higher than that in humans, suggesting that dextran 40 should not be used in domestic pigs.
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Choisunirachon N., Jaroensong T., Yoshida K., Saeki K., Mochizuki M., Nishimura R., Sasaki N., Nakagawa T.
Veterinary and Comparative Oncology 13 ( 4 ) 424 - 432 2015年12月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Veterinary and Comparative Oncology
Low-dose cyclophosphamide (CyLD) has shown promise in the treatment of several cancers; however, the effect of CyLD on canine oral malignant melanoma has never been explored. In this study, we investigated the effects of CyLD with or without piroxicam (Px) on tumour neovascularization and vascular normalization in a canine oral malignant melanoma-xenografted mice model. After treatment with CyLD, Px or a combination of both (CyPx), the growth of the tumour in the treatment groups was significantly suppressed compared to the control group at 30days of treatment. Proliferation index was also significantly reduced by all treatments, only CyPx significantly lowered microvessel density and vascular endothelial growth factor (VEGF) levels. Additionally, CyLD significantly reduced the proportion of normal vessels and caused an imbalance between VEGF and thrombospondin-1. These results suggested that CyPx has potent anti-angiogenic effects in terms of both the number and quality of blood vessels in xenografted canine oral malignant melanoma.
DOI： 10.1111/vco.12059
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460. Very Low Prevalence of Circulating Antibodies Against Common AAV Serotypes in a Population of Cats 査読あり
Kota Yoshida ∙ Kei Adachi ∙ Gregory A. Dissen ∙ Alejandro Lomniczi ∙ Sergio R. Ojeda ∙ Hiroyuki Nakai
Molecular Therapy 23 ( 1 ) S182 - S183 2015年5月
担当区分：筆頭著者 記述言語：英語 掲載種別：研究論文（国際会議プロシーディングス）