|
Affiliation |
Faculty of Agriculture Region of Animal and Plant Biosciences |
|
Title |
Professor |
|
External Link |
|
|
Related SDGs |
|
SAEKI Yuichi
|
|
|
Degree 【 display / non-display 】
-
Doctor (Agriculture) ( 1996.3 Kyushu University )
Research Areas 【 display / non-display 】
-
Life Science / Plant nutrition and soil science
-
Life Science / Applied microbiology
Papers 【 display / non-display 】
-
Impact of Inoculations with Indigenous Bradyrhizobium diazoefficiens Isolates on Productivity and Competition with Indigenous Bradyrhizobia in Adzuki Bean (Vigna angularis) Reviewed
Sokichi Shiro, Ryu Makihara, Shota Nakao, Masayuki Kadowaki, Yuichi Saeki
Microbes and Environments 40 ( 1 ) ME24069 2025.3
Authorship：Last author Language：English Publishing type：Research paper (scientific journal)
-
Soybean gene expression correlated with symbiotic rhizobial nitrogen fixation activity Reviewed
Yano Shunichi, Takushima Teruya, Ezawa Tatsuhiro, Sugimura Yusaku, Yamamoto Akihiro, Saeki Yuichi
Plant Root 18 35 - 47 2024.12
Authorship：Last author, Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：根研究学会
Soybean (<i>Glycine max</i> L.) can establish a symbiotic relationship with rhizobia. However, little is known about the host plant genes that correlate with the nitrogen fixation activity of symbiotic rhizobia. In this study, we investigated soybean genes that correlate with bacterial symbiotic nitrogen fixation in two experiments. In the first experiment, soybean seeds were inoculated with two strains of soybean rhizobia exhibiting different nitrogen fixation activities and were grown in the field until the R1 stage. Subsequently, the roots and nodules were subjected to RNA-seq analysis to identify the expression of soybean genes related to bacterial nitrogen fixation. In the second experiment, to confirm host gene expressions dependent on bacterial nitrogen fixation activity, nod<sup><sup>+</sup></sup>/fix<sup><sup>－</sup></sup> (Δ<i>nif</i> gene) mutants and the wild type were inoculated into soybean, and gene expressions were evaluated by qRT-PCR. <i>GmNRT2.5</i>, a high-affinity nitrate transporter gene, was correlated with nitrogen fixation activity. qPCR analysis of roots and nodules inoculated with a nod<sup><sup>+</sup></sup>/fix<sup><sup>－</sup></sup> mutant revealed that <i>GmNRT2.5</i> on chromosome 8 (Glyma.08G284000: <i>GmNRT2.5</i> Chr8) was particularly correlated with nitrogen fixation activity in the root nodule section. Based on the results, it is suggested that <i>GmNRT2.5</i> Chr8 may function as a regulatory gene bridging nitrogen fixation and nitrate absorption from the soil. Therefore, <i>GmNRT2.5</i> Chr8 might be a useful host gene for estimating symbiotic nitrogen fixation activity.
-
Effect of harvest time of day on the isoflavone synthesis pathway in immature soybeans Reviewed International coauthorship
Ismayawati, A.D., Yokoyama, D., Nishiguchi, Y., Saeki, Y., Sakakibara, H.
Food Research 8 ( 6 ) 411 - 417 2024.12
Language：English Publishing type：Research paper (scientific journal)
-
Effect of Drought Stress and Short-term Recovery on Proline and Polyamine Metabolism in Lotus japonicus Reviewed International coauthorship
Lee Y., Yamamoto A., Ragaey M., Hashiguchi M., Akashi R., Saeki Y.
Agricultural Research 2024.12
Authorship：Last author Language：English Publishing type：Research paper (scientific journal) Publisher：Agricultural Research
Environmental issues, particularly drought and soil salinity, severely limit plant productivity. This study aimed to examine the general reaction of legumes, specifically Lotus japonicus, against drought stress and their recovery following rehydration. After a 5-week culturing period, drought stress was simulated by withholding irrigation for 7 days. Stress response and recovery were evaluated through bioassays, and measurements of chlorophyll, proline and polyamine (stress reactants), and hydrogen peroxide and malondialdehyde (oxidative stress indices). Drought stress inhibited overall growth and decreased relative water content, but a recovery to control levels was observed after 8 h of re-watering. Hydrogen peroxide and malondialdehyde levels increased under drought stress, but showed no significant difference from control levels upon recovery. The accumulation of proline and polyamines was also observed, suggesting that they are effective responses to drought stress in plants. Furthermore, gene expression of key enzymes involved in proline and polyamine metabolism was regulated by stress conditions. By a short 8-h recovery period, the expression levels of metabolism-related genes of proline and polyamine returned to the pre-stress level, but the accumulation of functional substances was not induced. This study provides insight into the physiological and biochemical changes that occur in plants under drought stress and highlights the importance of proline and polyamine metabolism in plant adaptation to drought stress.
-
Effects of NaCl Treatment on Root Nodule Formation, Isoflavone Secretion in Soybean, and Nodulation Gene Expression in Rhizobia Reviewed International journal
Yoshikazu Nitawaki, Takaaki Yasukochi, Shinya Naono, Akihiro Yamamoto, and Yuichi Saeki
Microbes and Environments 39 ( 4 ) 2024.12
Authorship：Last author, Corresponding author Language：English Publishing type：Research paper (scientific journal)
We herein investigated the effects of salt (NaCl) stress on soybean nodulation by rhizobial strains. We specifically examined: (1) the effects of NaCl on nodule maturity and positioning by inoculating three rhizobial strains (Bradyrhizobium diazoefficiens USDA110T, Bradyrhizobium elkanii USDA31, and Sinorhizobium fredii USDA191) onto soybean variety CNS, (2) the effects of the NaCl treatment on isoflavones (daidzein and genistein) secretion by CNS, (3) the effects of the NaCl treatment on gene expression induced by daidzein and genistein in rhizobia, and (4) the effects of the NaCl treatment on rhizobial growth. The results obtained were as follows: (1) the NaCl treatment delayed nodule development and reduced nodulation on the primary root following the USDA110T inoculation, minimal sensitivity regarding nodule formation in the USDA 31 inoculation, and significantly increased the mature nodule number and nodules on the primary root following the USDA 191 inoculation. (2) The NaCl treatment significantly reduced the secretion of daidzein from soybean roots, but did not significantly affect that of genistein. (3) NaCl treatment induced a significant decrease in genistein-induced nodC expression in USDA110T, but not in USDA31, and also caused a significant reduction in daidzein-induced nodC expression, but not genistein-induced expression, in USDA191. (4) NaCl treatment reduced survivability under acidic conditions, but increased survivability under saline-alkaline conditions for USDA191 than bradyrhizobia. These results indicate that saline conditions give S. fredii a competitive advantage over Bradyrhizobium during soybean infection.
Books 【 display / non-display 】
-
日本の土壌事典
佐伯雄一 他（ Role： Joint author , 転換畑と大豆生産）
朝倉書店 2023.9
Language：Japanese Book type：Dictionary, encyclopedia
-
Soybean - Recent Advances in Research and Applications "jointly worked"
（ Role： Joint author）
InTech 2022.3
Language：English Book type：Scholarly book
-
Arakawa Y, Kusaba T, Kubotera H, Uezono I, Miyamaru N, Saeki Y, Niimi H, Nakano K, Koga N, Hara Y, Yamada Y, Inoue K, Ihara H, Nagatomo M, Yoshida K, Yamaguchi N, Hayashi H, Kondo T, Yamane T, Akagi I, Kinjo K, Shima T, Mitsugi K（ Role： Joint author , 10.2.3 Soybean-Rhizobium Management）
Springer 2021.2
Total pages：372 Responsible for pages：337-339 Language：English Book type：Scholarly book
Most soybean production in Saga and Fukuoka Prefec- tures is conducted on upland paddy fields. This agricultural system involves the management of soybean Bradyrhizobia, both to increase the soybean yield and to mitigate the emission of N2O gas from soybean fields. If soybean ger- mination damage caused by excess soil water can be avoided by the construction of ridges in the field, the rotational crop production of paddy rice and soybean will allow good agricultural performance for Asian monsoon regions.
-
Maria Luisa Tabing Mason, Yuichi Saeki（ Role： Joint author , Distribution and Characterization of the Indigenous Soybean- Nodulating Bradyrhizobia in the Philippines）
InTech 2019.4
Language：English Book type：Scholarly book
10.5772/intechopen.85709
-
Soil Microbiology
Yuichi Saeki（ Role： Joint author）
2018.8
Total pages：194 Responsible for pages：51-74 Language：Japanese Book type：Scholarly book
MISC 【 display / non-display 】
-
シバ属の耐塩性機構の解明と塩類集積土壌への適応の評価 Invited
山本昭洋，佐伯雄一
アグリバイオ 7 ( 1 ) 94 - 98 2023.1
Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media) Publisher：北隆館
-
根粒菌生態機能を活用した環境保全型ダイズ栽培技術の開発 Invited
佐伯雄一
作物生産と土づくり 55 ( 572 ) 21 - 26 2023.1
Authorship：Lead author Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media)
-
環境傾度によるダイズ根粒菌の群集生態に関する研究 Invited Reviewed
佐伯雄一
土と微生物 76 ( 2 ) 43 - 48 2022.10
Authorship：Lead author, Corresponding author Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (scientific journal)
-
シバ属遺伝資源を用いた耐塩性機構の解明と持続可能な地球環境への活用 Invited
山本昭洋，佐伯雄一
アグリバイオ 6 ( 5 ) 474 - 478 2022.5
Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media) Publisher：北隆館
-
ダイズ根粒菌の生態研究を環境傾度対応型ダイズ生産技術へ Invited
佐伯雄一，山本昭洋
アグリバイオ 3 ( 12 ) 1159 - 1162 2019.11
Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media) Publisher：北隆館
Presentations 【 display / non-display 】
-
塩ストレス条件下におけるS. frediiダイズ根粒菌の根粒着生優占化機構に関する研究
仁田脇義和・安河内孝晃・直野晋也・山本昭洋・佐伯雄一
日本土壌肥料学会 2024年度九州支部例会 2024.12.13
Event date： 2024.12.12 - 2024.12.13
Language：Japanese Presentation type：Oral presentation (general)
-
窒素と亜鉛の異なる施肥量がチャ葉の成分におよぼす影響
永野美希・河野美加・中嶋周作・児﨑章憲・山本昭洋・佐伯雄一
日本土壌肥料学会 2024年度九州支部例会 2024.12.12
Event date： 2024.12.12 - 2024.12.13
Language：Japanese Presentation type：Oral presentation (general)
-
ダイズ根粒菌混合接種による窒素固定量のコントロール －宿主の遺伝子発現による窒素固定活性評価の試み－
矢野俊一・山本昭洋・佐伯雄一
日本土壌肥料学会 2024年度九州支部例会 2024.12.12
Event date： 2024.12.12 - 2024.12.13
Language：Japanese Presentation type：Oral presentation (general)
-
塩ストレス条件下におけるシバ属の有用性評価
藤 康樹・山本昭洋・橋口正嗣・佐伯雄一・明石 良
日本土壌肥料学会 2024年度九州支部例会 2024.12.12
Event date： 2024.12.12 - 2024.12.13
Language：Japanese Presentation type：Oral presentation (general)
-
土地利用によるダイズ感染根粒菌群集構造の比較解析 International coauthorship
矢野俊一・長友由希・ Lee Yong Jae・Maria Luisa T. Mason・川口文隆・山本昭洋・佐伯雄一
日本土壌肥料学会 2024年度福岡大会 2024.9.4
Event date： 2024.9.3 - 2024.9.5
Language：Japanese Presentation type：Poster presentation
Awards 【 display / non-display 】
-
日本土壌肥料学会九州支部学術賞
2017.9 日本土壌肥料学会九州支部 根粒菌のゲノム生態学的研究
佐伯雄一
Award type：Award from Japanese society, conference, symposium, etc. Country：Japan
-
日本土壌肥料学会2012年度鳥取大会 ポスター賞
2012.9 日本土壌肥料学会
城惣吉, 松浦祥太, 山本昭洋, Gilbert C. Sigua, 佐伯雄一
Award type：Award from Japanese society, conference, symposium, etc. Country：Japan
-
日本土壌肥料学会奨励賞
2008.4 日本土壌肥料学会
佐伯雄一
Award type：International academic award (Japan or overseas) Country：Japan
Grant-in-Aid for Scientific Research 【 display / non-display 】
-
土壌のダイズ根粒菌群集構造と根粒占有率の優占化に関する研究
Grant number：22K05369 2022.04 - 2025.03
日本学術振興会 科学研究費基金 基盤研究(C)
佐伯雄一
Authorship：Principal investigator
-
環境傾度に基づくフィリピンと日本の根粒菌ゲ ノム多様性と群集構造の比較解析 International coauthorship
Grant number：21KK0103 2021.10 - 2026.03
日本学術振興会 科学研究費基金 国際共同研究加速基金（国際共同研究強化(B))
佐伯雄一・山本昭洋・城惣吉
Authorship：Principal investigator
-
微生物共生制御のロバスト性と環境応答モデリング：野外トランスクリプトミクスの応用
Grant number：20H02883 2020.04 - 2023.03
独立行政法人日本学術振興会 科学研究費補助金 基盤研究(B)
Authorship：Coinvestigator(s)
-
フィリピンの土着ダイズ根粒菌のゲノム多様性と群集構造解析
Grant number：18K05376 2018.04 - 2021.03
科学研究費補助金 基盤研究(C)
Authorship：Principal investigator
-
安心・多収・良食味を実現するサツマイモの地域適応型エンドファイト利用技術の開発
Grant number：16KT0032 2016.04 - 2019.03
科学研究費補助金 基盤研究(B)
Available Technology 【 display / non-display 】
-
有用ダイズ根粒菌の接種技術開発
ダイズ根粒菌のゲノム多型および環境経度による群集生態研究
宮崎県の資源を活用した代替肥料による作物栽培Home Page： 土壌肥料学研究室
Related fields where technical consultation is available：土壌化学性分析・施肥試験・植物微生物相互作用・群集構造解析
Message：土壌の理化学性や環境因子と土壌微生物の生態に興味を持って研究しています。土壌分析や栽培試験など取り組みたい希望があれば声を掛けてください。