Papers - SAEKI Yuichi
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Effect of Drought Stress and Short-term Recovery on Proline and Polyamine Metabolism in Lotus japonicus Reviewed International coauthorship
Lee Y., Yamamoto A., Ragaey M., Hashiguchi M., Akashi R., Saeki Y.
Agricultural Research 15 105 - 118 2026.3
Authorship：Last author Language：English Publishing type：Research paper (scientific journal) Publisher：Agricultural Research
Environmental issues, particularly drought and soil salinity, severely limit plant productivity. This study aimed to examine the general reaction of legumes, specifically Lotus japonicus, against drought stress and their recovery following rehydration. After a 5-week culturing period, drought stress was simulated by withholding irrigation for 7 days. Stress response and recovery were evaluated through bioassays, and measurements of chlorophyll, proline and polyamine (stress reactants), and hydrogen peroxide and malondialdehyde (oxidative stress indices). Drought stress inhibited overall growth and decreased relative water content, but a recovery to control levels was observed after 8 h of re-watering. Hydrogen peroxide and malondialdehyde levels increased under drought stress, but showed no significant difference from control levels upon recovery. The accumulation of proline and polyamines was also observed, suggesting that they are effective responses to drought stress in plants. Furthermore, gene expression of key enzymes involved in proline and polyamine metabolism was regulated by stress conditions. By a short 8-h recovery period, the expression levels of metabolism-related genes of proline and polyamine returned to the pre-stress level, but the accumulation of functional substances was not induced. This study provides insight into the physiological and biochemical changes that occur in plants under drought stress and highlights the importance of proline and polyamine metabolism in plant adaptation to drought stress.
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Effects of Soil Moisture Content and Rj Genotype Differences on Soybean Productivity and Soybean-nodulating Bradyrhizobial Occupancy Reviewed
Soto Ito1, Shingo Matsumoto, Masayuki Kadowaki, Hirokazu Sato, Yuichi Saeki, and Sokichi Shiro
Microbes and Environments 41 ( 1 ) 2026.3
Language：English Publishing type：Research paper (scientific journal)
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Unexpected Occurrence of Triploids and Preliminary Assessment of Genetic Relationships Using SSR Markers in Rubus parvifolius on Amami Oshima and Kakeroma, Japan Reviewed
Saki Toshima · Kodai Egashira · Makiko Mimura · Yuichi Saeki · Tomonari Hirano · Hisato Kunitake
Plant Molecular Biology Reporter 44 56 2026.2
Language：English Publishing type：Research paper (scientific journal)
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Differential salt stress responses and the role of polyamine metabolism in Lotus japonicus ecotypes Reviewed International coauthorship
Yongjae Lee, Akihiro Yamamoto, Chika Kuranaga-Kubo, Marwa Ragaey, Masatsugu Hashiguchi, Ryo Akashi, Yuichi Saeki
Discover Plants 2 252 2025.8
Authorship：Last author Language：English Publishing type：Research paper (scientific journal)
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Impact of Inoculations with Indigenous Bradyrhizobium diazoefficiens Isolates on Productivity and Competition with Indigenous Bradyrhizobia in Adzuki Bean (Vigna angularis) Reviewed
Sokichi Shiro, Ryu Makihara, Shota Nakao, Masayuki Kadowaki, Yuichi Saeki
Microbes and Environments 40 ( 1 ) ME24069 2025.3
Authorship：Last author Language：English Publishing type：Research paper (scientific journal)
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Soybean gene expression correlated with symbiotic rhizobial nitrogen fixation activity Reviewed
Yano Shunichi, Takushima Teruya, Ezawa Tatsuhiro, Sugimura Yusaku, Yamamoto Akihiro, Saeki Yuichi
Plant Root 18 35 - 47 2024.12
Authorship：Last author, Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：根研究学会
Soybean (<i>Glycine max</i> L.) can establish a symbiotic relationship with rhizobia. However, little is known about the host plant genes that correlate with the nitrogen fixation activity of symbiotic rhizobia. In this study, we investigated soybean genes that correlate with bacterial symbiotic nitrogen fixation in two experiments. In the first experiment, soybean seeds were inoculated with two strains of soybean rhizobia exhibiting different nitrogen fixation activities and were grown in the field until the R1 stage. Subsequently, the roots and nodules were subjected to RNA-seq analysis to identify the expression of soybean genes related to bacterial nitrogen fixation. In the second experiment, to confirm host gene expressions dependent on bacterial nitrogen fixation activity, nod<sup><sup>+</sup></sup>/fix<sup><sup>－</sup></sup> (Δ<i>nif</i> gene) mutants and the wild type were inoculated into soybean, and gene expressions were evaluated by qRT-PCR. <i>GmNRT2.5</i>, a high-affinity nitrate transporter gene, was correlated with nitrogen fixation activity. qPCR analysis of roots and nodules inoculated with a nod<sup><sup>+</sup></sup>/fix<sup><sup>－</sup></sup> mutant revealed that <i>GmNRT2.5</i> on chromosome 8 (Glyma.08G284000: <i>GmNRT2.5</i> Chr8) was particularly correlated with nitrogen fixation activity in the root nodule section. Based on the results, it is suggested that <i>GmNRT2.5</i> Chr8 may function as a regulatory gene bridging nitrogen fixation and nitrate absorption from the soil. Therefore, <i>GmNRT2.5</i> Chr8 might be a useful host gene for estimating symbiotic nitrogen fixation activity.
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Effect of harvest time of day on the isoflavone synthesis pathway in immature soybeans Reviewed International coauthorship
Ismayawati, A.D., Yokoyama, D., Nishiguchi, Y., Saeki, Y., Sakakibara, H.
Food Research 8 ( 6 ) 411 - 417 2024.12
Language：English Publishing type：Research paper (scientific journal)
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Effects of NaCl Treatment on Root Nodule Formation, Isoflavone Secretion in Soybean, and Nodulation Gene Expression in Rhizobia Reviewed International journal
Yoshikazu Nitawaki, Takaaki Yasukochi, Shinya Naono, Akihiro Yamamoto, and Yuichi Saeki
Microbes and Environments 39 ( 4 ) 2024.12
Authorship：Last author, Corresponding author Language：English Publishing type：Research paper (scientific journal)
We herein investigated the effects of salt (NaCl) stress on soybean nodulation by rhizobial strains. We specifically examined: (1) the effects of NaCl on nodule maturity and positioning by inoculating three rhizobial strains (Bradyrhizobium diazoefficiens USDA110T, Bradyrhizobium elkanii USDA31, and Sinorhizobium fredii USDA191) onto soybean variety CNS, (2) the effects of the NaCl treatment on isoflavones (daidzein and genistein) secretion by CNS, (3) the effects of the NaCl treatment on gene expression induced by daidzein and genistein in rhizobia, and (4) the effects of the NaCl treatment on rhizobial growth. The results obtained were as follows: (1) the NaCl treatment delayed nodule development and reduced nodulation on the primary root following the USDA110T inoculation, minimal sensitivity regarding nodule formation in the USDA 31 inoculation, and significantly increased the mature nodule number and nodules on the primary root following the USDA 191 inoculation. (2) The NaCl treatment significantly reduced the secretion of daidzein from soybean roots, but did not significantly affect that of genistein. (3) NaCl treatment induced a significant decrease in genistein-induced nodC expression in USDA110T, but not in USDA31, and also caused a significant reduction in daidzein-induced nodC expression, but not genistein-induced expression, in USDA191. (4) NaCl treatment reduced survivability under acidic conditions, but increased survivability under saline-alkaline conditions for USDA191 than bradyrhizobia. These results indicate that saline conditions give S. fredii a competitive advantage over Bradyrhizobium during soybean infection.
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Compatibility of adzuki bean (Vigna angularis) and Bradyrhizobium USDA strains, and geographical distribution and community structure on indigenous adzuki bean-nodulating bradyrhizobia in Japan Reviewed
Sokichi Shiro, Ryu Makihara, Masahiro Yamaguchi, Masayuki Kadowaki, Yuichi Saeki
Plant Protection Science 59 ( 3 ) 217 - 232 2023.9
Authorship：Last author Language：English Publishing type：Research paper (scientific journal)
DOI： 10.17221/64/2022-PPS
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Land utilization types impacted the species diversity and population distribution of nodulating and non-nodulating rhizobia in the Philippines Reviewed International coauthorship
Maria Luisa T. Mason, Baby Lyn T. De Guzman, Ariel G. Mactal, Akihiro Yamamoto , Yuichi Saeki
Rhizosphere 27 2023.7
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)
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Development of an effective method of human DNA extraction from soil as forensic evidence Reviewed International coauthorship
Hiroko Hirashima, Rinnosuke Hisazumi, Maria Luisa T. Mason, Akihiro Yamamoto, Yuichi Saeki
Forensic Science International 335 2022.3
Authorship：Last author, Corresponding author Language：English Publishing type：Research paper (scientific journal)
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栽培温度がダイズの生育および感染ダイズ根粒菌の群集構造に及ぼす影響 Reviewed
城 惣吉，間塚真矢，門脇正行，佐伯雄一
日本土壌肥料学雑誌 92 ( 3 ) 255 - 262 2021.6
Authorship：Last author Language：Japanese Publishing type：Research paper (scientific journal)
地球温暖化に伴う気候変動の作物生産への影響に関する基礎的知見の蓄積の一環として，異なる栽培温度環境 下におけるダイズの生育と有用ダイズ根粒菌の接種効果および感染ダイズ根粒菌群集構造への影響について調査 を行った.供試ダイズ品種として，‘オリヒメ’，‘ボンミノリ’，‘フクユタカ’ を用い，供試菌株として Bradyrhizo- bium diazoefficiens USDA110T を用いた.栽培は温度傾斜型チャンバー内で行い，低温区，中温区，高温区を設 けた.異なる温度環境下での栽培試験の結果，無接種区と比較して，USDA110 の接種により主茎長，主茎節数， 茎葉乾物重，莢数，莢乾物重，地上部乾物重，根粒数が有意に増加したが，栽培温度の上昇により根粒数を除く項 目が有意に減少した.各調査項目間の相関係数は根粒数と茎葉乾物重との関係など多くの組み合わせにおいて有意 な正の相関関係を示した.USDA110 の根粒占有率は，全ての温度区で接種した USDA110 が優占したが，低温区 と中温区，低温区と高温区を比較すると栽培温度の上昇により減少する傾向を示した.以上の結果から，高温環境 下では根粒数が減少することでダイズの生育が抑制され，その後の生育や収量に影響すると考えられた.しかし， 根粒数と USDA110 の根粒占有率を確保することができれば栽培温度の上昇によるダイズの生産性低下を抑制する ことができると考えられる.
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Effect of salt stress on soybean growth and nodulation under inoculation with soybean rhizobia Reviewed
Yoshikazu Nitawaki, Hayato Kitabayashi, Maria Luisa Tabing Mason, Akihiro Yamamoto, Yuichi Saeki
Soil Science and Plant Nutrition 67 ( 2 ) 103 - 113 2021.5
Authorship：Last author, Corresponding author Language：English Publishing type：Research paper (scientific journal)
Salinity is one of the major problems when it comes to soil fertility and agricultural productivity. In this regard, this study aimed to elucidate the influence of four salinity levels (0, 20, 50, 100 mM NaCl) on the soybean growth and the nodulation by rhizobia inoculated on the soybean variety CNS. Additionally, the compatibility of soybean varieties with the seven representative strains of rhizobia was also investigated. Through the compatibility test, it was revealed that the Sinorhizobium (Ensifer) fredii USDA 191 and the three bradyrhizobia (Bradyrhizobium japonicum USDA 6T, B. elkanii USDA 31, and B. diazoefficiens USDA 110T) showed compatible nodulation with the Rfg1 genotype soybean, whereas, the S. fredii USDA 192, 193, and 205T showed incompatible nodulation. The inoculation tests with bradyrhizobia (USDA 31, 110T) and sinorhizobia (USDA 191, 192, 193) indicated a better growth of soybean for bradyrhizobia than the sinorhizobia until 50 mM NaCl. Note that even though the symbiotic efficiency at each strain was not different among 0, 20, and 50 mM NaCl treatments, the symbiotic efficiency of bradyrhizobia was higher than the sinorhizobia at each NaCl treatment. The result of the mix inoculation test indicated that the nodule occupancy rate of strain USDA 191 increased significantly along with the NaCl concentration but strain USDA 110T showed an opposite response. These results indicate a positive relationship between the soybean growth and the nitrogen fixation ability of the inoculant under salt-stress conditions. Furthermore, the results obtained from the mix inoculation test partly describe the dominance of sinorhizobia under saline conditions.
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Maria Luisa T. Mason, Baby Lyn T. De Guzman, Akihiro Yamamoto, Yuichi Saeki
Symbiosis 83 ( 1 ) 55 - 63 2021.1
Authorship：Last author, Corresponding author Language：English Publishing type：Research paper (scientific journal)
This report aimed to evaluate the ability of the dominant indigenous strains of bradyrhizobia obtained from different locations in the Philippines in terms of their symbiosis, N-fixation and nodulation with various soybean cultivars harboring different Rj genotypes. This was done to select the most efficient and effective strain that can be used as an inoculant under the Philippines’ local condition. Two soybean cultivars from the Philippines and three cultivars from Japan and Brazil were used and inoculated with 12 indigenous strains and the reference strain Bradyrhizobium diazoefficiens USDA110. Culture pots were grown inside a growth chamber for four weeks with 33 °C for 16 h as daytime and 28 °C for 8 h as night time. All the strains formed nodules on all soybean cultivars, except for B. elkanii BO-4, which only formed nodules on Philippines’ cultivars. Among the indigenous strains, B. elkanii IS-2 is significantly the most efficient and effective N-fixer than the other strains for the Philippines’ cultivars. In contrast, B. diazoefficiens SK-5 was found to be the most efficient and effective N-fixer for cultivars from Japan and Brazil. Additionally, a positive correlation was observed between the symbiotic efficiency and the nitrogenase activity indicating an efficient N-fixation by the indigenous strains. Thus, we were able to identify the most promising indigenous bradyrhizobia that could be used as an inoculant to increase the soybean yield in the Philippines and provided insights on soybean inoculation.
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Latitudinal Characteristic Nodule Composition of Soybean-Nodulating Bradyrhizobia: Temperature-Dependent Proliferation in Soil or Infection? Reviewed
Md Hafizur Rahman Hafiz, Ahsanul Salehin, Fumihiko Adachi, Masayuki Omichi, Yuichi Saeki, Akihiro Yamamoto, Shohei Hayashi, Kazuhito Itoh
Horticulturae 2021.1
Language：English Publishing type：Research paper (scientific journal)
Abstract: A species-specific latitudinal distribution of soybean rhizobia has been reported; Bradyrhi- zobium japonicum and B. elkanii dominate in nodules in northern and southern areas, respectively. The aim of this study was to elucidate whether temperature-dependent proliferation in soil or infec- tion is more reliable for determining the latitudinal characteristic distribution of soybean-nodulating rhizobia under local climate conditions. Three study locations, Fukagawa (temperate continental climate), Matsue and Miyazaki (humid sub-tropical climate), were selected in Japan. Each soil sample was transported to the other study locations, and soybean cv. Orihime (non-Rj) was pot-cultivated using three soils at three study locations for two successive years. Species composition of Bradyrhi- zobium in the nodules was analyzed based on the partial 16S rRNA and 16S–23S rRNA ITS gene sequences. Two Bradyrhizobium japonicum (Bj11 and BjS10J) clusters and one B. elkanii (BeL7) cluster were phylogenetically sub-grouped into two (Bj11-1-2) and four clusters (BjS10J-1-4) based on the ITS sequence. In the Fukagawa soil, Bj11-1 dominated (80–87%) in all study locations. In the Matsue soil, the composition was similar in the Matsue and Miyazaki locations, in which BeL7 dominated (70–73%), while in the Fukagawa location, BeL7 decreased to 53% and Bj11-1 and BjS10J-3 increased. In the Miyazaki soil, BeL7 dominated at 77%, and BeL7 decreased to 13% and 33% in the Fukagawa and Matsue locations, respectively, while BjS10J-2 and BjS10J-4 increased. It was supposed that the B. japonicum strain preferably proliferated in the Fukagawa location, leading to its nodule domi- nancy, while in the Miyazaki location, temperature-dependent infection would lead to the nodule dominancy of B. elkanii, and both factors would be involved in the Matsue location.
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品評会における煎茶の評価とアミノ酸、カテキン類、テオガリン、没食子酸およびカフェインとの関係 Reviewed
児崎章憲，山本昭洋，佐伯雄一
茶業研究報告 129 11 - 18 2020.8
Language：Japanese Publishing type：Research paper (scientific journal)
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品評会における煎茶の評価と茶葉中無機元素含有量との関係 Reviewed
児崎章憲，山本昭洋，佐伯雄一
茶業研究報告 129 19 - 26 2020.8
Language：Japanese Publishing type：Research paper (scientific journal)
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Okamura Y, Morimoto N, Ikeda D, Mizusawa N, Watabe S, Miyanishi H, Saeki Y, Takeyama H, Aoki T, Kinoshita M, Kono T, Sakai M, Hikima J
Frontiers in Immunology 11 425-1 - 425-17 2020.3
Language：English Publishing type：Research paper (scientific journal)
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Metagenomic study of endophytic bacterial community of sweet potato (Ipomoea batatas) cultivated in different soil and climatic conditions Reviewed
Ramesh Raj Puri, Fumihiko Adachi, Masayuki Omichi, Yuichi Saeki, Akihiro Yamamoto, Shohei Hayashi, Md Arshad Ali, Kazuhito Itoh
World Journal of Microbiology and Biotechnology 35 ( 176 ) 1 - 8 2019.10
Language：English Publishing type：Research paper (scientific journal)
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Culture-dependent analysis of endophytic bacterial community of sweet potato (Ipomoea batatas) in different soils and climates Reviewed
Ramesh Raj Puri, Fumihiko Adachi, Masayuki Omichi, Yuichi Saeki, Akihiro Yamamoto, Shohei Hayashi, Kazuhito Itoh
Journal of Advances in Microbiology 13 ( 2 ) 1 - 12 2018.11
Language：English Publishing type：Research paper (scientific journal)
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Influence of flooding and soil properties on the genetic diversity and distribution of indigenous soybean-nodulating bradyrhizobia in the Philippines Reviewed
Maria Luisa Tabing Mason, Baby Lyn Cortez Tabing, Akihiro Yamamoto, Yuichi Saeki
Heliyon 4 2018.11
Authorship：Last author, Corresponding author Language：English Publishing type：Research paper (scientific journal)
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Genetic diversity of indigenous soybean-nodulating Bradyrhizobium elkanii from southern Japan and Nueva Ecija, Philippines Reviewed
Mason M., Matsuura S., Domingo A., Yamamoto A., Shiro S., Sameshima-Saito R., Saeki Y.
Plant and Soil 417 349 - 362 2017.8
Authorship：Last author, Corresponding author Language：English Publishing type：Research paper (scientific journal)
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Effect of Flooding and the nosZ Gene in Bradyrhizobia on Bradyrhizobial Community Structure in the Soil Reviewed
Yuichi Saeki, Misato Nakamura, Maria Luisa T. Mason, Tsubasa Yano, Sokichi Shiro, Reiko Sameshima-Saito, Manabu Itakura, Kiwamu Minamisawa, Akihiro Yamamoto
Microbes and Environments 32 ( 2 ) 154 - 163 2017.6
Authorship：Lead author, Corresponding author Language：English Publishing type：Research paper (scientific journal)
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2010年に宮崎で発生した口蹄疫の防疫措置終了後に農場内に留置された家畜排泄物ウイルス残存性評価 Reviewed
國保健浩, 真瀬昌司, 亀山健一郎, 池口厚男, 田中康男, 古田和生, 山田俊治, 大橋誠一, 深井克彦, 盛岡一樹, 小野里洋行, 堀井洋一郎, 西脇亜也, 飛佐学, 佐伯雄一, 井戸田幸子, 石井康之, 山本直之, 上村涼子, 末吉益雄, 川嶌健司
産業動物臨床医学誌 7 1 - 8 2016.6
Language：Japanese Publishing type：Research paper (scientific journal)
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The relationship between salt gland density and sodium accumulation/secretion in a wide selection from three Zoysia species Reviewed
Akihiro Yamamoto, Masatsugu Hashiguchi, Ryo Akune, Takahito Masumoto, Melody Muguerza, Yuichi Saeki, Ryo Akashi
Australian Journal of Botany 64 ( 4 ) 277 - 284 2016.6
Language：English Publishing type：Research paper (scientific journal)
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Temperature-Dependent Expression of NodC and Community Structure of Soybean-Nodulating Bradyrhizobia Reviewed
Sokichi Shiro, Chika Kuranaga, Akihiro Yamamoto, Reiko SameShima-Saito, Yuichi Saeki
Microbes and Environments 31 ( 1 ) 27 - 32 2016.1
Authorship：Last author, Corresponding author Language：English Publishing type：Research paper (scientific journal)
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Aung Zaw Htwe, Yuchi Saeki, Kyi Moe, Takeo Yamakawa
American Journal of Plant Sciences 6 2799 - 2810 2015.11
Language：English Publishing type：Research paper (scientific journal)
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Sulfation of Acetaminophen by the Human Cytosolic Sulfotransferases: A Systematic Analysis Reviewed
Akihiro Yamamoto, Ming-Yih Liu, Katsuhisa Kurogi, Yoichi Sakakibara, Yuichi Saeki, Masahito Suiko, Ming-Cheh Liu
Journal of Biochemistry 158 497 - 504 2015.11
Language：English Publishing type：Research paper (scientific journal)
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Functional genetic analysis of Arabidopsis thaliana SYNC1 in Lotus corniculatus super-growing roots using the FOX gene-funting system Reviewed
Tsubasa Yano, Hidenori Tanaka, Taiki Kurino, Akihiro Yamamoto, Hisato Kunitake, Yuichi Saeki, Ryo Akashi
Plant Root 9 6 - 14 2015.3
Language：English Publishing type：Research paper (scientific journal)
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The phenomenon of root elongation and high respiration activity in the rolB-gene-enhanced FSL#35 variant of Lotus corniculatus FOX-SR line Reviewed
Tsubasa Yano, Akihiro Yamamoto, Hisato Kunitake, Yuichi Saeki, Ryo Akashi
Plant Root 8 82 - 91 2014.12
Language：English Publishing type：Research paper (scientific journal)
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Correlation of soil types with N2O reductase genotype (nosZ) of indigenous soybean bradyrhiozbia: nosZ minus populations dominated in Andosol Reviewed
Yoko Shiina, Manabu Itakura, Choi Hyunseok, Yuichi Saeki, Masahito Hayatsu, Kiwamu Minamisawa
Microbes and Environments 29 ( 4 ) 420 - 426 2014.12
Language：English Publishing type：Research paper (scientific journal)
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A Thaumatin-Like Protein, Rj4, Controls Nodule Symbiotic Specificity in Soybean
Masaki Hayashi, Sokichi Shiro, Hiroyuki Kanamori, Satomi Mori-Hosokawa, Harumi Sasaki-Yamagata, Takashi Sayama, Miki Nishioka, Masakazu Takahashi, Masao Ishimoto, Yuichi Katayose, Akito Kaga, Kyuya Harada, Hiroshi Kouchi, Yuichi Saeki, Yosuke Umehara
Plant Cell Physiology 55 ( 9 ) 1679 - 1689 2014.9
Language：English Publishing type：Research paper (scientific journal)
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Effect of carbon sources on nitrogenase activity of diazotroph endophytic bacteria Reviewed
Akiko Kawakami, Masato Tanida, Tsubasa Yano, Sokichi Shiro, Yuichi Saeki, Akihiro Yamamoto
Bulletin of the Faculty of Agriculture, University of Miyazaki 60 - 13-20 2014.3
Language：Japanese Publishing type：Research paper (bulletin of university, research institution)
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Mathematical Ecology Analysis of Geographical Distribution of Soybean-Nodulating Bradyrhizobia in Japan Reviewed
Yuichi Saeki, Sokichi Shiro, Toshiyuki Tajima, Akihiro Yamamoto, Reiko Sameshima-Saito, Takashi Sato, Takeo Yamakawa
Microbes and Environments 28 ( 4 ) 470 - 478 2013.12
Authorship：Lead author, Corresponding author Language：English Publishing type：Research paper (scientific journal)
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Genetic diversity and geographical distribution of indigenous soybean-nodulating bradyrhizobia in the United States Reviewed
Sokichi Shiro, Syota Matsuura, Rina Saiki, Gilbert C. Sigua, Akihiro Yamamoto, Yosuke Umehara, Masaki Hayashi, Yuichi Saeki
Applied and Environmental Microbiology 79 ( 12 ) 3610 - 3618 2013.6
Authorship：Last author, Corresponding author Language：English Publishing type：Research paper (scientific journal)
DOI： 10.1128/AEM.00236-13
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豚糞尿スラリーを原料としたメタン発酵消化液の微生物群集構造 Reviewed
佐伯雄一、園田亮一、城 惣吉、矢野 翼、赤木 功、山本昭洋、杉本安寛
宮崎大学農学部研究報告 59 19 - 28 2013.3
Language：Japanese Publishing type：Research paper (bulletin of university, research institution)
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Effect of Rj Genotype and Cultivation Temperature on the Community Structure of Soybean-Nodulating Bradyrhizobia
Sokichi Shiro, Akihiro Yamamoto, Yosuke Umehara, Masaki Hayashi, Naoto Yoshida, Aya Nishiwaki, Takeo Yamakawa, Yuichi Saeki
Applied and Environmental Microbiology 78 ( 4 ) 1243 - 1250 2012.2
Language：English Publishing type：Research paper (scientific journal)
DOI： 10.1128/AEM.06239-11
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Phylogenetic diversity of indigenous cowpea bradyrhizobia from soils in Japan based on sequence analysis of the 16S-23S rRNA internal transcribed spacer (ITS) region Reviewed
Papa S. Sarr, Yamakawa T, Saeki Y. Guisse A
Systematic and Applied Microbiology 34 ( 4 ) 285 - 292 2011.6
Language：English Publishing type：Research paper (scientific journal)
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Changes in Population Occupancy of Bradyrhizobia under Different Temperature Regimes Reviewed
Yuichi Saeki, Satoru Ozumi, Akihiro Yamamoto, Yosuke Umehara, Masaki Hayashi, Gilbert C. Sigua
Microbes and Environments 25 ( 4 ) 309 - 312 2010.12
Authorship：Lead author, Corresponding author Language：English Publishing type：Research paper (scientific journal)
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Catalytic biomineralization of fluorescent calcite by the thermophilic bacterium Geobacillus thermoglucosidasius Reviewed
Yoshida N, Higashimura E, Saeki Y
Applied and Environmental Microbiology 76 ( 21 ) 7322 - 7327 2010.11
Language：English Publishing type：Research paper (scientific journal)
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Development of multiple-occupancy nodules in correlation of the density-dependent infection by soybean nodulating rhizobia Reviewed
Minh Thi Nguyen, Akiyoshi K, Nakatsukasa M, Saeki Y, Yokoyama K.
Soil Microorganisms 64 ( 2 ) 101 - 106 2010.10
Language：English Publishing type：Research paper (scientific journal)
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Multiple occupancy of nodules by nodulating rhizobia on field-grown soybeans with attendance of Sinorhizobium spp. Reviewed
Minh Thi Nguyen, Kazue Akiyoshi, Masamichi Nakatsukasa, Yuichi Saeki, Kazuhira Yokoyama
Soil Science and Plant Nutrition 56 382 - 389 2010.8
Language：English Publishing type：Research paper (scientific journal)
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豚糞尿を原料としたメタン発酵消化液の液肥としての安全性に関する微生物的評価 Reviewed
園田亮一、赤木功、浅野陽樹、山本昭洋、佐伯雄一
宮崎大学農学部研究報告 56 93 - 101 2010.2
Language：Japanese Publishing type：Research paper (bulletin of university, research institution)
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ダイズ根粒菌の外来遺伝子獲得に関する菌株間差異の解析 Reviewed
城惣吉、山本昭洋、吉田ナオト、佐伯雄一
宮崎大学農学部研究報告 56 79 - 87 2010.2
Language：Japanese Publishing type：Research paper (bulletin of university, research institution)
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ニガウリの緑色長円筒形系統と白色紡錘形系統間の交雑F2世代における果長、果径、果実色およびL-アスコルビン酸含量 Reviewed
赤木功、井野寿俊、佐伯雄一
宮崎大学農学部研究報告 56 89 - 92 2010.2
Language：Japanese Publishing type：Research paper (bulletin of university, research institution)
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Intragenomic variation of internal transcribed spacer regions between 16S-23S rRNA genes among the three copies of Sinorhizobium fredii strains Reviewed
Yuichi Saeki, Hiroko Oguro, Isao Akagi, Takeo Yamakawa, Akihiro Yamamoto
Soil Science and Plant Nutrition 55 627 - 633 2009.10
Authorship：Lead author, Corresponding author Language：English Publishing type：Research paper (scientific journal)
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Phylogenetic diversity and symbiotic effectiveness of root-nodulating bacteria associated with cowpea in the south-west area of Japan Reviewed
Sarr PP, Yamakawa T, Fujimoto S, Saeki Y, Thao HTB, Myint MK
Microbes and Environments 24 ( 2 ) 105 - 112 2009.6
Language：English Publishing type：Research paper (scientific journal)
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Effect of late sowing on the isoflavone content of soybean seed in Southern Kyushu Reviewed
Akagi I, Nishihara M, Ueda S, Yokoyama A, Saeki Y
Japanese Journal of Crop Science 78 ( 2 ) 250 - 254 2009.4
Language：Japanese Publishing type：Research paper (scientific journal)
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Estimation of nodulation tendency among Rj-genotype soybeans using the bradyrhizobial community isolated from an Andosol Reviewed
Minami M, Yamakawa T, Yamamoto A, Akao S, Saeki Y.
Soil Science and Plant Nutrition 55 65 - 72 2009.2
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)
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Mineral contents in seeds of soybean cultivars bred for warm region in Japan Reviewed
Isao Akagi, Motoki Nishihara, Shigehide Ueda, Akitoshi Yokoyama, Yuichi Saeki
Bulletin of the Faculty of Agriculture, University of Miyazaki 55 91 - 98 2009.2
Language：Japanese Publishing type：Research paper (bulletin of university, research institution)
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The estimation of the amount of nitrogen fixation in the sugarcane inoculated diazotrophic endophyto in field Reviewed
Kazumi Nakamura, Masato Tanida, Seiichiro Sonoda, Kana Udonishi, Yuichi Saeki, Shoichiro Akao, Akihiro Yamamoto
Bulletin of the Faculty of Agriculture, University of Miyazaki 55 99 - 108 2009.2
Language：Japanese Publishing type：Research paper (bulletin of university, research institution)
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Genomic comparison of Bradyrhizobium japonicum strains with different symbiotic nitrogen-fixing capabilities and other Bradyrhizobiaceae members Reviewed
Itakura M, Saeki K, Omori H, Yokoyama T, Kaneko T, Tabata S, Ohwada T, Tajima S, Uchiumi T, Honnma K, Fujita K, Iwata H, Saeki Y, Hara Y, Ikeda S, Eda S, Mitsui H, Minamisawa K.
The ISME Journal 3 326 - 339 2009.1
Language：English Publishing type：Research paper (scientific journal)
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Estimation of bacterial community diversity of soybean-nodulating bradyrhizobia isolated from Rj-genotype soybeans Reviewed
Saeki Y, Minami M, Yamamoto A, Akao S
Soil Science and Plant Nutrition 54 718 - 724 2008.10
Authorship：Lead author, Corresponding author Language：English Publishing type：Research paper (scientific journal)
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Colonization and growth promotion characteristics of Enterobacter sp. and Herbaspirillum sp. on Brassica oleracea Reviewed
Zakria M, Ohsako A, Saeki Y, Yamamoto A, Akao S
Soil Science and Plant Nutrition 54 507 - 516 2008.8
Language：English Publishing type：Research paper (scientific journal)
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Infection, multiplication and evaluation of the nitrogen-fixing ability of Herpaspirillum sp. strain B501gfp1 in sugarcane stems inoculated by the vacuum infiltration method Reviewed
Zakria M, Udonishi K, Saeki Y, Yamamoto, A, Akao S
Microbes and Environments 23 128 - 133 2008.5
Language：English Publishing type：Research paper (scientific journal)
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Influence of inoculation technique on the endophytic colonization of rice by Pantoea sp. isolated from sweet potato and Enterobacter sp. from sugarcane Reviewed
Zakria M, Udonishi K, Ogawa T, Yamamoto A, Saeki Y, Akao S
Soil Science and Plant Nutrition 54 224 - 236 2008.4
Language：English Publishing type：Research paper (scientific journal)
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Diversity and Distribution of Indigenous Soybean-Nodulating Rhizobia in the Okinawa Islands, Japan Reviewed
Suzuki K, Oguro H, Yamakawa T, Yamamoto A, Akao S, Saeki Y
Soil Science and Plant Nutrition 54 237 - 246 2008.4
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)
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Differentiation of soybean-nodulating Bradyrhziobium USDA strains based on restriction fragment length polymorphisms of 23S-5S rRNA genes Reviewed
Saeki Y, Murata T, Yamakawa T, Akao S.
Soil Science and Plant Nutrition 53 562 - 567 2007.10
Authorship：Lead author, Corresponding author Language：English Publishing type：Research paper (scientific journal)
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Colonization and nitrogen-fixing ability of Herbaspirillum sp. strain B501 gfp1 and assessment of its growth-promoting ability in cultivated rice Reviewed
Zakria M, Njoloma J, Saeki Y, Akao S
Microbes and Environment 22 ( 3 ) 197 - 206 2007.9
Language：English Publishing type：Research paper (scientific journal)
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Polyphenol contents, radical-scavenging activity and isoflavone contents of soybean (Glycine max (L.) Merr.) seeds as affected by insect injury Reviewed
Isao Akagi, Motoki Nishihara, Shigehide Ueda, Akitoshi Yokoyama, Yoki Asano, Yuichi Saeki
78 ( 4 ) 399 - 402 2007.8
Language：Japanese Publishing type：Research paper (scientific journal)
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ハウス栽培キュウリの生理傷害に及ぼす亜鉛と銅の影響 Reviewed
川崎佳栄, 長友慎太郎, 横山明敏, 佐伯雄一, 赤尾勝一郎
日本土壌肥料学雑誌 78 ( 2 ) 143 - 148 2007.7
Language：Japanese Publishing type：Research paper (scientific journal)
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Isoflavone content of soybean cultivars for warm districts grown in Miyazaki prefecture Reviewed
Isao Akagi, Motoki Nishihara, Shigehide Ueda, Akitoshi Yokoyama, Yoki Asano, Yuichi Saeki
76 ( 3 ) 454 - 458 2007.7
Language：Japanese Publishing type：Research paper (scientific journal)
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Antimicrobial activity of the zoo compost produced from hog feces against plant pathogenic microorganisms Reviewed
Ryo Ohshima, Yuichi Saeki, Kenichi Tsuchiya, Shoichiro Akao
Bulletin of the Faculty of Agriculture, University of Miyazaki 53 ( 1·2 ) 85 - 91 2007.3
Language：Japanese Publishing type：Research paper (bulletin of university, research institution)
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Diversity and geographical distribution of indigenous soybean-nodulating bradyrhizobia in Japan Reviewed
Saeki Y, Aimi N, Tsukamoto S, Yamakawa T, Nagatomo Y, Akao S.
Soil Science and Plant Nutrition 52 418 - 426 2006.8
Authorship：Lead author, Corresponding author Language：English Publishing type：Research paper (scientific journal)
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The microorganism activity in the sediment by supplying saturated dissolved oxygen water Reviewed
Sumio Masuda, Hiroki Goto, Masahito Yamauchi, Tomoo Sekito, Yuichi Saeki
35 197 - 203 2006.8
Language：Japanese Publishing type：Research paper (bulletin of university, research institution)
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Incorporation of a DNA sequence encoding green fluorescent protein (GFP) into endophytic diazotroph from sugarcane and sweet potato and the colonizing ability of these bacteria in Brassica oleracea Reviewed
Tanaka K, Shimizu T, Zakria M, Njoloma JP, Saeki Y, Sakai M, Yamakawa T, Minamisawa K, Akao S.
Microbes and Environments 21 ( 2 ) 122 - 128 2006.7
Language：English Publishing type：Research paper (scientific journal)
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Colonization ability of Herbaspirillum spp. B501gfp1 in sugarcane, a non-host plant in the presence of indigenous diazotrophic endophytes Reviewed
Njoloma JP, Oota M, Taroura K, Saeki Y, Akao S.
African Journal of Biotechnology 5 ( 10 ) 836 - 841 2006.5
Language：English Publishing type：Research paper (scientific journal)
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イネにおける不定根の発生とその伸長に及ぼす亜鉛とオーキシンの影響 Reviewed
井上昭彦, 鵜戸西夏奈, 佐伯雄一, 長友由隆, 赤尾勝一郎
宮崎大学農学部研究報告 52 ( 1-2 ) 57 - 64 2006.3
Language：Japanese Publishing type：Research paper (bulletin of university, research institution)
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Morphology and classification of nematodes in Andosols Reviewed
Chigusa Yamashita, Eiji Kawano, Yoshitaka Nagatomo, Yucihi Saeki
Bulletin of the Faculty of Agriculture, University of Miyazaki 52 ( 1-2 ) 47 - 55 2006.3
Language：Japanese Publishing type：Research paper (bulletin of university, research institution)
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サトウキビに接種した窒素固定エンドファイトHerbaspirillumの動態追跡のための緑色蛍光タンパク質の利用 Reviewed
清水友, 豊里学, 久綱泰代, 佐伯雄一, 南澤究, 中西康博, Njoloma JP, 明石良, 赤尾勝一郎
土と微生物 60 ( 1 ) 3 - 9 2006.2
Language：Japanese Publishing type：Research paper (scientific journal)
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Detection of gfp expression from gfp-labelled bacteria spot inoculated onto sugarcane tissues Reviewed
Njoloma JP, Oota M, Saeki Y, Akao S.
African Journal of Biotechnology 4 ( 12 ) 1372 - 1377 2005.12
Language：English Publishing type：Research paper (scientific journal)
-
Phylogenetic analysis of soybean-nodulating Rhizobia isolated from alkaline soils in Vietnam Reviewed
Saeki Y, Kaneko A, Hara T, Suzuki K, Yamakawa T, Nguyen MT, Nagatomo Y, Akao S.
Soil Science and Plant Nutrition 51 ( 7 ) 1043 - 1052 2005.12
Authorship：Lead author, Corresponding author Language：English Publishing type：Research paper (scientific journal)
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15N同位体希釈法によるサトウキビの固定窒素量の推定 Reviewed
西口友広, 清水友, Njoloma JP, 大田守也, 佐伯雄一, 赤尾勝一郎
宮崎大学農学部研究報告 51 ( 1-2 ) 53 - 62 2005.3
Language：Japanese Publishing type：Research paper (bulletin of university, research institution)
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カボチャにおけるオーキシンで誘導される発根とカルス化に及ぼす亜鉛の影響 Reviewed
横山明敏, 佐伯雄一, 小口慶子, 長友由隆, 赤尾勝一郎, 高木浩
根の研究 13 ( 4 ) 143 - 147 2004.12
Language：Japanese Publishing type：Research paper (scientific journal)
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亜鉛及び銅過剰がキュウリの子づる・孫づる形成に及ぼす影響 Reviewed
横山明敏, 佐伯雄一, 柴田聡子, 長友由隆, 赤尾勝一郎
日本土壌肥料学雑誌 50 ( 4 ) 475 - 478 2004.8
Language：Japanese Publishing type：Research paper (scientific journal)
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Grouping of Bradyrhizobium USDA strains by sequence analysis of 16S rDNA and 16S-23S rDNA internal transcribed spacer region Reviewed
Saeki Y, Aimi N, Hashimoto M, Tsukamoto S, Kaneko A, Yoshida N, Nagatomo Y, Akao S.
Soil Science and Plant Nutrition 50 ( 4 ) 517 - 525 2004.8
Authorship：Lead author, Corresponding author Language：English Publishing type：Research paper (scientific journal)
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Chestnut bur-shaped aggregates of chrysotile particles enable inoculation of Escherichia coli cells with plasmid DNA Reviewed
Yoshida N., Saeki Y.
Applied Microbiology and Biothechnology 65 566 - 575 2004.7
Language：English Publishing type：Research paper (scientific journal)
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イネにおけるオーキシンの発根促進作用に及ぼす亜鉛の影響 Reviewed
横山明敏, 佐伯雄一, 小口慶子, 長友由隆, 高木浩, 赤尾勝一郎
日本土壌肥料学雑誌 75 ( 2 ) 211 - 216 2004.4
Language：Japanese Publishing type：Research paper (scientific journal)
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Chrysotile fibers penetrate Escherichia coli cell membrane and cause cell bursting by sliding friction force on agar plates Reviewed
Yoshida N, Saeki Y
Journal of Bioscience and Bioengineering 97 ( 3 ) 162 - 168 2004.3
Language：English Publishing type：Research paper (scientific journal)
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芋製焼酎粕施用農耕地における硝酸態窒素濃度の経時的変化-第II報.圃場試験- Reviewed
佐伯雄一、中村扶沙恵、三重野愛、下入佐克志、赤尾勝一郎、杉本安寬、長友由隆
宮崎大学農学部研究報告 50 ( 1-2 ) 31 - 39 2004.3
Language：Japanese Publishing type：Research paper (bulletin of university, research institution)
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芋製焼酎粕施用農耕地における硝酸態窒素濃度の経時的変化-第I報.現地調査- Reviewed
佐伯雄一、下入佐克志、三重野愛、赤尾勝一郎、杉本安寬、長友由隆
宮崎大学農学部研究報告 50 ( 1-2 ) 41 - 48 2004.3
Language：Japanese Publishing type：Research paper (bulletin of university, research institution)
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西洋ミヤコグサ由来“Super-growing Root” (Lotus corniculatus L.) の発根・伸長に及ぼす亜鉛添加の効果 Reviewed
小口慶子, 井上昭彦, 明石良, 佐伯雄一, 長友由隆, 赤尾勝一郎, 高木浩
日本土壌肥料学雑誌 75 ( 1 ) 9 - 14 2004.2
Language：Japanese Publishing type：Research paper (scientific journal)
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Detection of plant parasitic nematodes, Meloidogyne incognita and Pratylenchus coffeae by multiplex PCR using specific primers Reviewed
Saeki Y, Kawano E, Yamashita C, Akao S, Nagatomo Y.
Soil Science and Plant Nutrition 49 ( 2 ) 291 - 295 2003.4
Authorship：Lead author, Corresponding author Language：English Publishing type：Research paper (scientific journal)
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Study on seeding rice in nutrient solution
Le, T.B.D. and Saeki, Y.
Plant Nutrition for Sustainable Agricultural Development 47 - 51 2001.12
Language：English Publishing type：Research paper (international conference proceedings)
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Comparison of nitrogen content among soybean varieties
Saeki, Y. and Nguyen, M.T.
Plant Nutrition for Sustainable Agricultural Development 23 - 30 2001.12
Language：English Publishing type：Research paper (international conference proceedings)
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Diversity of indigenous Bradyrhizobium strains isolated from three different Rj-soybean cultivars in terms of randomly amplified polymorphic DNA and intrinsic antibiotic resistance Reviewed
Saeki Y, Akagi I, Takaki H, Nagatomo Y.
Soil Science and Plant Nutrition 46 ( 4 ) 917 - 926 2000.8
Authorship：Lead author, Corresponding author Language：English Publishing type：Research paper (scientific journal)
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Distinctive expression of a zinc-binding protein in rice callus grown in medium with high zinc concentration Reviewed
Saeki Y, Yasukouchi A, Nagatomo Y, Takaki H.
Soil Science and Plant Nutrition 46 ( 1 ) 209 - 206 2000.2
Authorship：Lead author, Corresponding author Language：English Publishing type：Research paper (scientific journal)
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Estimation of diversity of indigenous Bradyrhizobium japonicum strains in different types of soil by intrinsic antibiotic resistance Reviewed
Saeki Y, Sakasegawa H, Inoue Y, Akagi I, Nagatomo Y.
Bulletin of the Faculty of Agriculture, Miyazaki University 46 ( 1-2 ) 85 - 91 1999.3
Language：English Publishing type：Research paper (bulletin of university, research institution)
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Nodule formation and distribution of Rj2Rj3-genotype soybean infected with Bradyrhizobium japonicum Reviewed
Saeki Y, Yamakawa T, Ikeda M, Ishizuka J.
Journal of the Faculty of Agriculture. Kyushu University 43 ( 3-4 ) 317 - 326 1999.2
Authorship：Lead author, Corresponding author Language：English Publishing type：Research paper (bulletin of university, research institution)
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Molecular cloning, expression, and characterization of a novel mouse liver SULT1B1 sulfotransferase Reviewed
Yuichi Saeki, Yoichi Sakakibara, Yoichi Araki, Ken Yanagisawa, Masahito Suiko, Hiroshi Nakajima, Ming-Cheh Liu
Journal of Biochemistry 124 55 - 64 1998.1
Language：English Publishing type：Research paper (scientific journal)
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Molecular cloning and characterization of the mouse liver dopa/tyrosine sulfotransferase Reviewed
Saeki Y, Arafat Y, Sakakibara Y, Suio M, NAkajima AH, Liu M-C
FASEB Journal 11 ( 9 ) A883 1997
Language：English Publishing type：Research paper (scientific journal)
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Effects of root exudates of Rj2Rj3- and Rj4-genotype soybeans on growth and chemotaxis of Bradyrhizobium japonicum Reviewed
Saeki Y, Yamakawa T, Ikeda M, Ishizuka J.
Soil Science and Plant Nutrition 42 ( 2 ) 413 - 417 1996.4
Authorship：Lead author, Corresponding author Language：English Publishing type：Research paper (scientific journal)
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Accumulation of indole carboxylic acid in dwarf rice Tanginbozu
Hossain B, Sugita K, Saeki Y, Rahman S, Nagatomo Y, Tamai M, Takaki H
Proc. the International Colloquim on Impact of Plant Biotechnology on Agriculture. 223 - 238 1994.12
Language：English Publishing type：Research paper (international conference proceedings)